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INDONESIA
Jurnal Bioteknologi & Biosains Indonesia (JBBI)
Published by Badan Pengkajian dan Penerapan Teknologi
ISSN : 24422606     EISSN : 2548611X     DOI : -
Core Subject : Health, Science, Agriculture, Social,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Health Professions
JBBI is published twice annually and provide scientific publication medium for researchers, engineers, practitioners, academicians, and observers in the field related to biotechnology and bioscience. This journal accepts original research papers, review articles, case studies, and short communications. The articles published are peer-reviewed by no less than two referees, and cover various biotechnology subjects related to the field of agriculture, industry, health, environment, bioinformatics, as well as life sciences in general.
Arjuna Subject : Umum - Umum
Articles 177 Documents
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IN SILICO MOLECULAR TARGETING AND ADMET PROFILING OF BETA-SITOSTEROL AS A MULTI-TARGET THERAPEUTIC CANDIDATE Fendy Prasetyawan; Yuneka Saristiana; Lisa Savitri
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 13 No. 1 (2026)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

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Abstract

Beta-sitosterol is a naturally occurring phytosterol with potential biological activities, including anticancer effects. This study aimed to evaluate the pharmacokinetic characteristics and molecular targets of beta-sitosterol using in silico approaches involving ADMET analysis and target prediction. The ADMET results indicated that beta-sitosterol exhibited favorable membrane permeability and high absorption potential; however, it demonstrated poor aqueous solubility and high plasma protein binding, which may affect its bioavailability. Additionally, metabolic prediction suggested potential involvement of cytochrome P450 enzymes in beta-sitosterol biotransformation. Target prediction analysis revealed that beta-sitosterol may interact with multiple proteins involved in lipid metabolism and hormonal regulation. The highest predicted affinity was observed toward NPC1L1, a protein involved in cholesterol absorption, as well as nuclear receptors including LXR-α, ROR-γ, and androgen receptor, which regulate lipid metabolism and hormone signaling. Furthermore, beta-sitosterol was predicted to interact with HMG-CoA reductase and several cytochrome P450 isoforms involved in cholesterol biosynthesis and steroidogenesis. The findings suggest that beta-sitosterol possesses a multi-target profile associated with lipid metabolism and hormonal regulation pathways.
IN SILICO PREDICTION OF BIOACTIVE TARGET CLASSES AND ADMET PROFILE OF CAPSAICIN WITH RELEVANCE TO DAYAK CHILI (Capsicum frutescens L.) Yuneka Saristiana; Fendy Prasetyawan; Lisa Savitri
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 13 No. 1 (2026)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

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Abstract

Capsaicin is a well-known bioactive compound present in various Capsicum species, including Dayak chili (Capsicum frutescens L.). This study aimed to predict the bioactive target classes and ADMET profile of capsaicin using an in silico approach. The chemical structure of capsaicin was retrieved from the PubChem database and analyzed using SwissTargetPrediction, SwissADME, and pkCSM platforms. The prediction results indicated that capsaicin interacts with multiple protein classes, including oxidoreductases, cytochrome P450 enzymes, voltage-gated ion channels, G-protein-coupled receptors, hydrolases, phosphatases, kinases, and membrane receptors. High-probability predicted targets included PTGS1, CYP1A2, TRPV1, CNR1, and FAAH, suggesting potential anti-inflammatory, analgesic, neuromodulatory, and metabolic regulatory effects. ADMET analysis demonstrated favorable absorption and distribution characteristics, with predicted hepatotoxicity and hERG II inhibition requiring further investigation. These findings represent computational predictions and should be interpreted cautiously, as in silico results require further experimental validation. Capsaicin demonstrates multi-target potential that may support future pharmacological development and experimental studies.
SEX-DEPENDENT HEAT TOLERANCE PROFILES OF BALI CATTLE EXPOSED TO TROPICAL HEAT STRESS Kirana Dara Dinanti Adiputra; Sukandi; Karenina Dwi Yulianti; Nurliani Erni; Dani Nur Arifin
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 13 No. 1 (2026)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

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Abstract

Heat stress is one of the main challenges in livestock farming systems in tropical areas, directly affecting the physiological response and productivity of livestock. Bali cattle, as one of Indonesia's local germplasms, are known to have good adaptability to extreme environmental conditions. This study aims to evaluate the heat tolerance of male and female Bali cattle using the Iberian Heat Tolerance Coefficient (IHTC), Benezra’s Coefficient of Adaptability (BCA), and Thermal Stress Resistance Index (TSRI). Data analysis used the independent samples t-test to compare between male and female sexes under heat stress conditions (THI = 84.8–87.2). The results showed significant differences (P < 0.05) in IHTC and BCA values between male and female Bali cattle, while TSRI did not show significant differences (P > 0.05). Male Bali cattle had lower IHTC values and higher BCA values compared to female cattle. This finding indicates that female Bali cattle have relatively better heat tolerance compared to male Bali cattle.
ANALYSIS OF LACTIC ACID BACTERIA WEISSELLA PARAMESENTEROIDES LM-21 AS PROBIOTIC CANDIDATE Luthfia Muharram; Aflah Afina Nurdin; Rahma Risnaya; Sharen Ayuningtyas; Syifa Luthfia; Nurhaifa Azzahra
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 13 No. 1 (2026)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

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Abstract

Probiotics are live microorganisms that provide many health benefits. Weissella genus have been much research over this decade because probiotic potential. Weissella Paramesenteroides LM-21 isolated from Kimchi kiciwis leaf have health potential as glutathione antioxidant production. This research aims to analyze the ability of W. paramesenteroides LM-21 as a probiotic candidate.  The methodes are cell viability test at low pH (pH setting of 2.0; 3.0; 4.0; 7.0), antibacterial activity against E.coli and S.aureus, analysis production of exopolysaccharides (EPS) and qualitative analysis of short chain fatty acid (SCFA) by HPLC. The results are W.paramesentreroides LM-21 could survive at low pH (viability at pH 2 is 6.2 log CFU/mL); showed inhibition zone against E. coli was 0.8 mm and S. aureus was 0.5 mm; ability producing EPS with ropy phenotype and crude extract of 30 mg/mL; and SCFA (acetic acid based on a retention time of 10.688 minutes and propionic acid of 26.787 minutes). Based on the results of this research, can be concluded that W. paramesenteroides LM-21 has excellent potential as a probiotic candidate
THE EFFECT OF NASAL CANNULA USE ON BLOATING INCIDENTS AT KARSA HUSADA HOSPITAL, BATU CITY Dion Kunto Adi Patria; Riki Ristanto; Yuni Asri
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 13 No. 1 (2026)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

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Abstract

Oxygen administration via nasal cannula at a dose of 3-4 liters/minute is standard therapy for hypoxemic patients, but it has the potential to cause bloating due to air insufflation into the gastrointestinal tract. This study analyzed the relationship between nasal cannula use at a dose of 3-4 liters/minute for >8 hours and the incidence of bloating. The study used an observational analytical study with a cross-sectional design in 30 patients who received oxygen therapy via nasal cannula at a dose of 3-4 liters/minute for >8 hours at Karsa Husada Batu Hospital. Data were analyzed using a Chi-Square Test comparing the proportion of bloating before and after the intervention. Before the intervention, no patients reported bloating. After administration of 3-4 liters/minute for >8 hours, 26 respondents (86.7%) experienced bloating. The Chi-Square test showed a significant difference (χ²=24.04; df=1; p<0.05). Conclusion: Use of a nasal cannula at a dose of 3-4 liters/minute for >8 hours significantly increases the risk of bloating.
PROFILE OF CYTOTOXICITY IN HepG2 CELLS AND THE POTENTIAL FOR MODULATION OF PANCREATIC CELLS BY PINEAPPLE CROWN FRACTION (Ananas comosus (L.)Merr) IN DIABETIC RATS Winartiana Winartiana; Okky Intan Mawarni; Iftinah Harini; Erlina Putri Lestari
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 13 No. 1 (2026)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

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Abstract

The ethyl acetate fraction of pineapple crown, Ananas comosus (L.) Merr is known to contain flavonoid secondary metabolites with potential antidiabetic activity. Flavonoids have been widely reported to have potent antidiabetic effects through antioxidant activity and pancreatic cell-protective mechanisms. This study aimed to evaluate the in vitro toxicity profile of the ethyl acetate fraction using HepG2 cells and its in vivo antidiabetic efficacy in an alloxan-induced diabetic rat model. Cytotoxicity was assessed using the MTT assay, with Doxorubicin HCl used as a positive control. The in vivo study involved 35 male Wistar rats divided into seven groups: a negative control, a positive control (glibenclamide 0.45 mg/kgBW), and five treatment groups receiving the ethyl acetate fraction at doses ranging from 10 to 25 mg/kgBW. The evaluated parameters included changes in body weight, blood glucose levels, and pancreatic α- and β-cell counts. The cytotoxicity assay showed that the ethyl acetate fraction exhibited an IC50 value of 287.14 µg/ml, indicating relatively low toxicity compared with Doxorubicin HCl (IC50 =43.25 µg/ml). In vivo administration of the ethyl acetate fraction, particularly at a dose of 22 mg/kgBW, produced the most optimal effects, as indicated by body weight recovery toward normal values, a significant reduction in blood glucose levels, a decrease in pancreatic α-cell count (40±1.7), and an increase in β-cell number (48 ± 1.7) (p < 0.05). The ethyl acetate fraction of Ananas comosus (L.) Merr demonstrates antidiabetic potential by improving islet cell profiles while exhibiting moderate cytotoxicity in vitro, suggesting its potential for further development as an antidiabetic therapeutic agent.
INTERACTION OF SCALABILITY PROCESS WITH ENVIRONMENTAL LIMITATIONS ON MICROALGAE CULTIVATION: LIGHT COLOR AND SALINITY EFFECTS Nina Juliana Roberta Turnip; Rayke Dhiya F.; Yuelia Salsabilla; Ulil Afni; Lestari Lestari; Deviany Deviany; Andri Sanjaya
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 13 No. 1 (2026)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

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Abstract

Scaling up the cultivation of Chlorella vulgaris often results in differences in growth performance compared to laboratory-scale cultivation due to increased sensitivity to environmental factors. This study examined the interaction between light color, salinity, and the scale-up process at cultivation volumes of 1, 5, and 10 L using synthetic NaCl media and artificial seawater (ASW). Growth was monitored via optical density measurements. The results indicated that inoculum is a key factor factor influencing the success of scale-up. Cultures transferred during the logarithmic growth phase adapted more quickly and maintained exponential growth, while inoculum from the decline phase showed slower recovery and lower cell density. Red light minimized light stress and promoted higher cell density in both media. Lower salinity promoted faster growth and earlier peak attainment. Changes in dry biomass pigments indicate carotenoid accumulation under high salinity and light stress. The highest biomass yield was achieved when harvested during the logarithmic phase, with ASW medium providing higher productivity. Overall, optimizing inoculum age, light, and salinity is crucial for improving production efficiency during the C. vulgaris scale-up process.
IN SILICO ANALYSIS OF FIG (Ficus carica L.) BIOACTIVE COMPOUNDS AS MULTITARGET THERAPEUTIC CANDIDATE AGAINST HIV-1 Musa'adah Musa'adah; Ayuni Adawiyah; Raysha Tryfhatya Nurhaidha; Yani Suryani; Risda Arba Ulfa
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 13 No. 1 (2026)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

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Abstract

Antiretroviral drugs (ARV) to treat HIV-1 infection which causes AIDS are predominantly monotargeted so it is considered less effective. Long-term use of this drug may also lead to side effects and the HIV-1 resistance to drug. Thereby highlighting the need for developing more effective multitarget drug candidates. Herbal-based medicine have potential to be developed into multitarget drugs due to its diverse bioactive compounds. In silico approaches are used for initial screening in new drug development. This study aimed to evaluate the potential of fig (Ficus carica L.)-derived bioactive compounds as multitarget antiretroviral therapy candidates against three key HIV-1 proteins: Gp120, HIV-1 reverse transcriptase, and HIV-1 protease, in silico. Fourteen bioactive compounds consisting of anthocyanin, flavonoid, and terpenoid derivatives were analyzed for their physicochemical properties, pharmacokinetic profiles, toxicity, and molecular docking interactions. The results revealed that five compounds of flavonoid group: apigenin, catechin, epicatechin, kaempferol, and luteolin, fulfilled the criteria as potential oral multitarget drug candidates with relatively low binding affinity (ΔG) values toward all three HIV-1 target proteins. Notably, luteolin exhibited the strongest binding affinity toward Gp120 (-7.2 kcal/mol), HIV-1 reverse transcriptase (-8.8 kcal/mol) and HIV-1 protease (-8.5 kcal/mol), while also complying Lipinski’s, ADMET parameters, and low-toxicity predictions. These findings suggest that luteolin considerable as a safe natural-based multitarget antiretroviral candidate derived from Ficus carica L.
ANTIFUNGAL ACTIVITY POTENTIAL AND VOLATILE COMPOUND PROFILE OF Bacillus sp. HSFI-6 Extract Maya Dian Rakhmawatie; Mazidatu Khoirinnisa’ Adiyanti; Nur Fadhila Sari; Fadhil Widya Dharma Putra; Kanti Ratnaningrum; Ika Dyah Kurniati; Devita Diatri
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 13 No. 1 (2026)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

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Abstract

Antifungal resistance has implications for the development of bioactive compounds. Bacillus sp. has been widely studied for the discovery of antifungal volatile compounds. This study aims to explore the antifungal activity of Bacillus sp. HSFI-6 isolated from sea cucumber (Holothuria scabra Fermented Intestine-HSFI) against Malassezia furfur, Candida albicans, and Aspergillus fumigatus. Bacillus sp. HSFI-6 was identified molecularly by 16S rRNA sequencing. Ethyl acetate extract of Bacillus sp. HSFI-6 was produced using media containing starch, yeast, peptone, and its metabolites were profiled using Gas Chromatography-Mass Spectroscopy (GC-MS). A disc diffusion antifungal assay was performed. Bacillus sp. HSFI-6 has 93.47% similarities with Bacillus albus strain MCCC 102146 and moderately inhibit C. albicans (7.00 ± 1.15 mm), A. fumigatus (9.25 ± 0.96 mm). The GC-MS metabolomics analysis showed two antifungal volatile compounds, namely cycloheptasiloxane, tetradecamethyl- and cyclononasiloxane, octadecamethyl-. These results conclude that Bacillus sp. HSFI-6 is suitable for the development of antifungal drugs.
EFFECTIVENESS OF HEALTH EDUCATION ON FOOTWEAR SELECTION IN REDUCING FOOT ULCER INCIDENCE AMONG PATIENTS WITH TYPE 2 DIABETES MELLITUS Rizki Hidayat; Naziyah Naziyah; Masdiana Masdiana
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 13 No. 1 (2026)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

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Abstract

The incidence of diabetes mellitus in Indonesia is 10.8%. DM is a disease caused by the body's inability to produce insulin, which can lead to complications, one of the most common being foot ulcers. If foot conditions are not properly managed, they will worsen, leading to amputation. Prevention of foot ulcer. nvolves providing education on choosing footwear to reduce the incidence of foot injuries. This study aimed to determine the effect of footwear selection education on the incidence of foot ulcers among patients with type 2 diabetes mellitus.This research method   a quasi-experimental design with a single intervention group. The sample used was 30 respondents, with the research instrument being the Inlow's 60-second diabetic foot screen observation sheet. This study was conducted by collecting pre-test data using the observation sheet on type 2 diabetes mellitus patients participating in  Prolanis (Program Pengelolaan Penyakit Kronis), followed by education on footwear selection, evaluation, and post-test data collection over a month, with data processing using the Wilcoxon test.The results showed a decrease in the incidence of foot ulcers followinf the footwear selection education intervention. Statitical analysis indicated a significant effect of footwear selection education on reducing foot ulcer incidence among patients with type 2 diabetes mellitus (p<0.05). Education on footwear selection influences footwear choices among type 2 diabetes mellitus patients at the Pasar Minggu Community Health Centre. Type 2 diabetes mellitus patients are expected to implement the footwear selection provided not only for those undergoing treatment at the community health centre and prolanis but also at other healthcare facilities.

Page 17 of 18 | Total Record : 177

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