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INDONESIA
Jurnal Bioteknologi & Biosains Indonesia (JBBI)
Published by Badan Pengkajian dan Penerapan Teknologi
ISSN : 24422606     EISSN : 2548611X     DOI : -
Core Subject : Health, Science, Agriculture, Social,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Health Professions
JBBI is published twice annually and provide scientific publication medium for researchers, engineers, practitioners, academicians, and observers in the field related to biotechnology and bioscience. This journal accepts original research papers, review articles, case studies, and short communications. The articles published are peer-reviewed by no less than two referees, and cover various biotechnology subjects related to the field of agriculture, industry, health, environment, bioinformatics, as well as life sciences in general.
Arjuna Subject : Umum - Umum
Articles 146 Documents
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ANALYSIS OF BIOMETHANE PRODUCTION POTENTIAL AND MITIGATION OF BIOMETHANE EMISSIONS FROM PIG FARMS IN NORTH SULAWESI PROVINCE Efrando Manullang; Sutaryo; Endang Kusdiyantini
Jurnal Bioteknologi dan Biosains Indonesia Vol. 10 No. 2 (2023)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

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Abstract

The potential for biomethane production from livestock manure as renewable energy has good prospects, considering that the number of pigs in North Sulawesi Province increases yearly. Therefore, it is necessary to analyze the potential for biomethane production, replacing the use of LPG, and mitigate biomethane emissions from pig farms. The results showed that from 2015-2021, the most significant biomethane production was in 2021, amounting to 7,887 tons of CH4/year in the Minahasa Regency area, 2,496 tons of CH4/year (31.65%) in Tomohon City, 805 tons of CH4/year (10.20%) in South Minahasa Regency, 779 tons of CH4/year (9.88%), and in other areas 3,807 (48.27%). Biomethane can replace 8.59 million kg of Liquefied Petroleum Gas (LPG). The results of mitigating biomethane emissions in 2021 reached 34.22 Gg CO2eq, and using biomethane to replace LPG reached 11.59 Gg CO2eq. Total greenhouse gas emissions can be reduced to 45.81 Gg CO2eq. Biomethane from pig manure has promising potential as a new renewable energy and requires further investigation.
MOLECULAR IDENTIFICATION OF PHOSPHATE SOLUBILIZING BACTERIA FROM PGPR PRODUCT DERIVED FROM TEMANGGUNG REGENCY Muhammad Falih Akbar; Susiana Purwantisari; Sri Pujiyanto; Rejeki Siti Ferniah
Jurnal Bioteknologi dan Biosains Indonesia Vol. 10 No. 2 (2023)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

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Abstract

T Phosphate solubilizing bacteria are soil bacteria that can dissolve phosphates from insoluble phosphate bonds so that they can be absorbed by plants. Phosphates in the soil are naturally present in organic and inorganic forms. Both forms are insoluble or slightly soluble forms of phosphate, so their availability for soil biota is very limited. This study aimed to identify the phosphate solubilizing bacteria from locally PGPR product from Temanggung, Indonesia, namely BPF 1. The method in this study was carried out by molecular identification using the 16S rRNA encoding gene which includes bacterial DNA isolation, DNA amplification using PCR, bacterial DNA sequencing and the creation of phylogenetic trees. The results of the study were obtained isolate bacteria solubilizing phosphate BPF 1 from PGPR products of local farmers of Temanggung Regency identified as the genus Bacillus, having the closest kinship with B. subtilis strain SBMP4 (NR_118383.1) with a similarity of 98.42%.
IN-SILICO ANALYSIS OF SYMBIONT BACTERIA DIVERSITY IN THE MIDGUT OF Aedes aegypti USING 16S rDNA MOLECULAR MARKERS DATABASE Syubbanul Wathon; Aufar Finasrullah; Rike Oktarianti; Kartika Senjarini
Jurnal Bioteknologi dan Biosains Indonesia Vol. 10 No. 2 (2023)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

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Abstract

Dengue Hemorrhagic Fever (DHF) is caused by the dengue virus, which is transmitted through Aedes aegypti mosquitoes when they feed on human blood.  To effectively control the DHF vector, it is crucial to accurately characterize the symbiont bacteria associated with Ae. aegypti through an in-silico approach to identify potential targets. This study utilized in- silico analysis based on the 16S rDNA molecular marker to explore the diversity of symbiont bacteria obtained from bioinformatics databases. The analysis and visualization of bacterial diversity were conducted using the Pathosystem Resource Integration Center (PATRIC). The analysis results revealed that bacterial diversity in the midgut of Ae. aegypti, categorized as culturable and non-culturable bacteria, exhibited similar abundance patterns at the family level, albeit with varying detection rates. The most dominant taxa included the phylum Proteobacteria, class Gammaproteobacteria, order Enterobacterales, and family Enterobacteriaceae. Within the culturable bacteria category, the dominant taxa were the genus Salmonella and species Salmonella enterica, whereas the non-culturable bacteria category indicated the prevalence of the genus Escherichia and species Escherichia coli.
EVALUATION AND INHIBITORY MECHANISM ANALYSIS OF NATURAL COMPOUNDS AGAINTS DIHYDROOROTATE DEHYDROGENASE AS ANTI-CANCER AGENTS Aji Wibowo; Danang Waluyo; Bimo Ario Tejo; Tinta Komariyah; Ariza Yandwiputra Besari; Erwahyuni Endang Prabandari
Jurnal Bioteknologi dan Biosains Indonesia Vol. 10 No. 2 (2023)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

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Abstract

Cancer remains one of the deadliest diseases worldwide, and currently cancer treatment is facing several problems related to adverse effects and drug resistance. To address these problems, new prospective anticancer medications are required. Natural compounds, which have been extensively used in the drug research, including for the treatment of cancer, are emerging as viable candidates. This study aimed to evaluate 33 in-house natural compounds against dihydroorotate dehydrogenase (DHODH) enzyme, a viable target to develop anticancer agent, and to analyze the hit inhibitory mechanism against protein target. In the activity assay, atovaquone was the sole substance to have activity against DHODH, with an inhibition rate of 47.44% at 10 µM. However, discrepancies were shown in the molecular docking result, where atovaquone were identified as hits. Molecular dynamic analysis revealed that atovaquone initially bound to the active site before being forced to the outside due to cleavage of hydrogen bond between the ligand and responsible residue. This study clearly demonstrated the importance of molecular dynamic analysis to study inhibitory mechanism of compound against target protein that may be useful for further development.
INHIBITORY ACTIVITY OF INDONESIAN MICROBIAL EXTRACTS AGAINST PROLIFERATION OF DLD-1 COLORECTAL CANCER CELL LINE Ariza Yandwiputra Besari; Danang Waluyo; Tinta Komariyah; Erwahyuni Endang Prabandari
Jurnal Bioteknologi dan Biosains Indonesia Vol. 10 No. 1 (2023)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

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Abstract

Colorectal cancer (CRC) is the second deadliest cancer in the world. Several anti-cancer agents are currently used for the clinical treatment of CRC. However, toxicity and drug resistance pose significant challenges in CRC chemotherapy. On the other hand, microbe-derived natural products have been explored as a source for the development of anti-cancer therapeutic agents. This study aimed to examine the potential of the microbial library in BioMCC (Biotech Center-BPPT Microbial Culture Collection) as a source for anti-cancer drug discovery. Among the 720 fungal extracts tested, 60 extracts (8.3%) showed inhibitory activity against the proliferation of the colorectal carcinoma DLD-1 cell line, while not affecting Vero cells (African green monkey kidney normal cell line). One of these active extracts was derived from the fungus Sporothrix sp. BioMCC-f.T.7716. Although the inhibitory mechanism of this extract against the proliferation of the DLD-1 cell line could not be determined, this study clearly demonstrated the potential use of Indonesian microbial extracts as a source for the discovery of anti-cancer agents.
ANALYSIS OF KIDNEY HISTOPATHOLOGY IN SEPSIS MODEL OF MICE (MUS MUSCULUS) WITH PREVENTIVE TREATMENT OF LEAF EXTRACT FROM 'DAUN KENTUT' PLANT (PAEDERIA FOETIDA L.) AGAINST ESCHERICHIA COLI INFECTION Lisa Savitri; Elfred Rinaldo Kasimo; Rochmad Krissanjaya; Datin An Nisa Sukmawati; Syntia Tanu Juwita; Akbar Nur Kholis; Yosep Mosse1
Jurnal Bioteknologi dan Biosains Indonesia Vol. 10 No. 2 (2023)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

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Abstract

Sepsis is a critical health concern characterized by organ dysfunction due to uncontrolled host reactions to infections. The prevalence of microorganisms causing sepsis varies, with Gram-positive bacteria and fungal sepsis gaining significance. Sepsis often leads to acute kidney injury (AKI), and its association with sepsis is known as sepsis-associated AKI (SA-AKI). The "Daun Kentut" plant, known for its diverse bioactive compounds, has shown potential in combating inflammation and oxidative stress. In this study, histopathological changes in mouse kidneys induced by Escherichia coli were investigated. Hemorrhage was the primary change observed. Dehydration caused by E. coli infection potentially led to kidney injury through hormonal responses. The plant extract's anti-inflammatory potential, attributed to compounds like saponins and flavonoids, was also explored. The study emphasizes the need for understanding sepsis-related kidney damage and suggests possible preventive strategies using natural compounds.
BACTERIAL CONTAMINATION TEST IN POWDER-FORMULATED Helicoverpa armige-ra NUCLEAR POLYHEDROSIS VIRUS (HaNPV1) SUBCULTURE Mia Miranti; Hikmat Kasmara; Nurullia Fitriani; Melanie Melanie; Inas Qurrata A'yun; Yolani Syaputri; Febri Doni; Madihah Madihah; Sri Rejeki Rahayuningsih; Nabilah Sekar Azizah; Wawan Hermawan
Jurnal Bioteknologi dan Biosains Indonesia Vol. 10 No. 2 (2023)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

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Abstract

The Helicoverpa armigera Nuclear Polyhedrosis Virus (HaNPV1) is a subculture derived from the original HaNPV, and it has been cultivated in Spodoptera litura larvae as an alternative host. HaNPV1 was subsequently formulated using gypsum and talcum as carrier media. Following this formulation, a bacterial contamination test was conducted to assess the quality of the viral formulation.  The experiment was arranged in the randomized factorial block design (RFBD) with 2 replications. The viral formulations was stored for 16 weeks and the samples were taken every two weeks for contamination analysis. The data was then analyzed with the analysis of variance (ANOVA) and a post-hoc using Duncan’s Multiple Range test. The variable observed was the number of the bacterial colonies cultivated on the specific media i.e., Nutrient Agar (NA), Salmonella Shigella Agar (SSA) and Eosin Methilen Blue Agar (EMB). The results showed that the bacterial contaminants was detected from 0 to 12 weeks of storage time. However, the highest contamination was found in viral formulation after 8 weeks of storage time and the highest bacterial contaminations were recorded from all viral formulation tested in NA. The results indicated that the bacterial contamination were found around 1.45 × 109 cfu/gram and 1.97 × 109 cfu/gram in gypsum and talcum formulations, respectively. On SSA and EMB media, the bacteria contaminants from all formulation found in 8 weeks of storage time, but Salmonella, Shigella, or Escherichia coli (aspathogenic bacteria) were not found. After 12 weeks storage time, there was no indication of  contamination found in all media. Furthermore, Bacillus species was found as a most dominant contaminant in all samples. In conclusion, although the viral formulations using gypsum and talc were not contaminated by pathogenic bacteria such Salmonella, Shigella, or E. coli. Nevetherless, the viral formulation was still easily contaminated by other non-pathogenic bacterial species. Thus, a more standardized and stricted strategy needs to be developed for a better viral formulation product.
IDENTIFICATION OF INDONESIAN ETHNOMEDICINAL PLANTS AS POTENTIAL DRUG CANDIDATES FOR ACUTE RESPIRATORY INFECTION USING COMPUTER-AIDED DRUG DESIGN AND SIMRS MODEL Jackson, James; Tanaya, Davina Nadine; Setiawan, Stefanus
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 11 No. 1 (2024)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2024.2949

Abstract

In 2023, Indonesia’s air quality deteriorated, with its Air Quality Index (AQI) tripling clean air standards, causing health sector losses, including a surge in Acute Respiratory Infection (ARI) cases. One of the ARI treatments is the consumption of cefuroxime, yet it can cause side effects. Indonesia’s floral biodiversity in ethnomedicinal plants can be utilized as a more natural drug candidate for ARI drugs. To determine this, an in silico approach is performed through molecular docking, and Pre-ADMET prediction. Based on the compound selection’s results, lanosterol is the most promising compound, with a binding energy value of -8.11 kcal/mol and an efficiency of 78.81%, while cefuroxime as a reference ligand has a binding energy value of -5.92 kcal/mol with an efficiency of 67.87%. After undergoing compound selection, a time series analysis through the Susceptible Infected Medicine Recovered Susceptible (SIMRS) model is conducted. In this analysis, it is found that cefuroxime and lanosterol require the same amount of time, which is 33 days to restore Indonesia to its pre-ARI outbreak condition, indicating that lanosterol can be used as an alternative drug candidate.
STUDY OF ANTI-BACTERIAL AND ANTI-INFLAMMATORY PROPERTIES OF INDONESIA’S FLORA BIODIVERSITY AS DRUG CANDIDATES FOR DIABETIC ULCER THROUGH IN SILICO AND IN VITRO APPROACH Tiffanny, Cecilia; Regina, Aurelia; Chen, Davin Handreas; Rosyati, Margaretha Maria
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 11 No. 2 (2024)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2024.2950

Abstract

This study is driven by the substantial increase in the number of diabetes mellitus patients, which is projected to triple by 2030 and the immense potential of Indonesia's extensive biodiversity. Diabetic ulcers represent one of the most common complications stemming from uncontrolled diabetes mellitus. Therefore, this research aims to determine the therapeutic potential of Indonesian biodiversity for diabetic ulcers, focusing on the anti-bacterial and anti-inflammatory properties of secondary metabolite derivatives, employing both in silico and in vitro approaches. Initially, the research employed an in silico approach, conducting molecular docking and molecular dynamics simulations on reference ligands obtained from the 6KVS receptor for Staphylococcus aureus bacteria and the 5KIR for the COX-2 protein. Molecular docking was performed for 18 secondary metabolite derivative test compounds using Autodock Vina, followed by molecular dynamics simulations. The results of the molecular dynamics simulations showed that flavanonols exhibited the highest stability among the test compounds, with an average root mean squared deviation of 0.139493 Å for anti-bacterial tests and 0.182499 Å for anti-inflammatory tests after 50 ns simulation. Based on the results of molecular dynamics simulations, in vitro anti-bacterial and anti-inflammatory testing was conducted using flavanonol compounds found in Ananas comosus plants. In the anti-bacterial test, the pineapple extract, at a 5% concentration, exhibited the largest inhibitory zone diameter, 3.53 mm. In the anti-inflammatory tests, the pineapple extract at an optimal concentration of 600 ppm showed the highest absorbance value for protein denaturation, measuring 0.11133 au.
DETERMINATION OF OPTIMAL ANNEALING TEMPERATURE Vibrio alginolyticus PRIMERS USING POLYMERASE CHAIN REACTION METHOD Putri, Gladys Indira; Nurjayadi, Muktiningsih; Declan, Jefferson Lynford; Krisdawati, Ismaya; Juliansyah, Dandy Akbar; Fahriza, Tiara; Azzahra, Maharanianska; Maulana, Irvan; Kartika, Irma Ratna; Kurniadewi, Fera; Sukmawati, Dalia; Saamia4, Vira; Saputro, Dwi Anna Oktaviani; Wiranatha, I Made; Abomoelak, Bassam; El-Enshasy, Hesham Ali
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 10 No. 2 (2023)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2023.2976

Abstract

Food poisoning is a global issue of grave concern. If food is not properly cooked, it can be a medium for the spread of pathogenic bacteria. Vibrio alginolyticus is one of the pathogenic bacteria that can cause food poisoning. real-time Polymerase Chain Reaction (rt-PCR) can detect pathogenic bacteria in food, so it is necessary to determine the optimal annealing temperature. This research aims to obtain the optimal annealing temperature of the Va_Chr1_FR primer using Gradient PCR. The DNA concentration used was 174.5 with an A260/A280 purity of 1.94. The temperature range tested, 53°C-62°C, corresponds to the melting temperature of the Va_Chr1_FR primers. The primers designed were F5'-TTCTTCTGTTGTAGGTTCCG-F3' and R5'-CCAGCCCTCACATCTAATAC-R3'. Based on these results, a temperature of 60°C is deemed as the most optimal annealing temperature because it produces one of the brightest bands on electrophoresis with an amplicon length of 146 bp. The findings of this study will be beneficial to the development of Va_Chr1_FR Vibrio alginolyticus primers testing on food samples using the real-time PCR method. 

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