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Jurnal Bioteknologi & Biosains Indonesia (JBBI)
Published by Badan Pengkajian dan Penerapan Teknologi
ISSN : 24422606     EISSN : 2548611X     DOI : -
Core Subject : Health, Science, Agriculture, Social,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Health Professions
JBBI is published twice annually and provide scientific publication medium for researchers, engineers, practitioners, academicians, and observers in the field related to biotechnology and bioscience. This journal accepts original research papers, review articles, case studies, and short communications. The articles published are peer-reviewed by no less than two referees, and cover various biotechnology subjects related to the field of agriculture, industry, health, environment, bioinformatics, as well as life sciences in general.
Arjuna Subject : Umum - Umum
Articles 147 Documents
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OPTIMIZING AMOUNT AND IDENTIFICATION OF YEAST IN SALAK YEAST WATER (Salacca edulis Reinw cv Pondoh) Khumaira, Annisa; Annaziha, Salma; Baihaqi, Muhammad Azizan Azmani; Anindita, Nosa Septiana; Bimantara, Arif; Probowati, Wiwit
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 11 No. 1 (2024)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2024.3809

Abstract

Salak fruit is a tropical fruit spread across Southeast Asia and can potentially be a source of natural yeast by processing fruit yeast water. Salak fruit is known to be rich in carbohydrates, making it a potential natural habitat for microbial communities, especially yeast. This research aims to optimize the growth of yeast in the salak yeast water system by varying the sugar concentration treatment, the composition of the amount of fruit flesh, and the fermentation time, as well as identifying the yeast in the salak yeast water. The method used is to count the number of yeast colonies using the Total Plate Count method. The most optimal treatment results are then tested for pH, total sugar, alcohol, isolation and yeast identification. The research results showed that the composition of salak meat was 30%, adding 1% sugar, and fermentation for 6 days resulted in the most optimal growth of the microbial community with an amount of 3.1×10⁶ cfu/ml. The pH test showed a result of 3.01, the alcohol content, namely ethanol, was 0.066855%, no methanol was detected, the total sugar test result in the yeast water was 2.08%, and it was identified in the yeast water that there were Hanseniaspora opuntiae and Candida sorboxylosa. The results show that salak yeast water (Salacca edulis Reinw cv Pondoh) can be used as a yeast water product to ferment food.
THE SORUS MORPHOLOGY OF FERNS OF THE FAMILY Dryopteridaceae AND THE FAMILY Polypodiaceae THE TATANGGE UNIVERSITY FOREST, SSOTHEAST SULAWESI Munir, Asmawati; Damhuri; Samai, Suarna; Lisdayanti, Hikma; Lizawarni
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 11 No. 1 (2024)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2024.4845

Abstract

This study aims to determine the morphology of the sorus of ferns (Pteridophyta) Dryopteridaceae and Polypodiaceae families in the Tatangge Education Forest area of Rawa Aopa Watumohai National Park, Southeast Sulawesi. This research involved exploration in the Tatangge University Forest area of Rawa Aopa Watumohai National Park, Southeast Sulawesi to collect samples, then identifying and observing sorus characteristics in the Laboratory of the Biology Education Department, Faculty of Teacher Training and Education, Halu Oleo University, Kendari. The research data were processed and analysed descriptively, regarding the characteristics of the sorus of ferns (Pteridophyta) Dryopteridaceae family including location, shape, colour, and annulus. The results of the study found 5 (five) species of ferns (Pteridophyta) Dryopteridaceae family, and 5 (five) species of Polypodiaceae family that have different sorus characteristics. The Dryopteridaceae family consists of the species Dryopteris celsa, Dryopteris filix-mas, Dryopteris marginalis, Nephrolepis biserrata (Sw) Schoot, and Polistichopsis hasseltii. The family Polypodiaceae consists of the species Drynaria sparsisora (Desv.) T. Moore Pyrrosia longifollia (Burm. f), Pyrrosia lanceolata L Farw, Pyrrosia piloselloides M.G. Price, and Polypodium glycyrriza Licorice Fern.
TRANSDERMAL PATCH PILIS FROM TURMERIC RHIZOME AND BLACK SEEDS FOR TREATMENT BASED ON ETHNOPHARMACY Susanto, Nindita Clourisa Amaris; Sawitri, Arinda Handiyah; Muslimah, Intan
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 11 No. 2 (2024)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2024.4870

Abstract

Pilis, a traditional product containing various herbal ingredients, is currently less desir-able due to its impractical and unfashionable method of application. Pilis is used to treat dizziness, pain, eye fatigue, encephalitis in children, fever, and to aid recovery for mothers after childbirth. However, pilis also carries risks related to uncontrolled dosage and contamination, particularly from chemical or heavy metal pollutants, which may cause toxic reactions. To mitigate these risks, pilis should be used in recommended doses and produced under clean and safe conditions. This research aimed to provide the innovation of patches as an alternative treatment to increase attractiveness and control the active substances released and delivered for a long time. Transdermal patches are made from a combination of turmeric and black seed containing flavonoid active metabolite compounds, namely curcumin, carotenoids, quercetin, kaempferol, and apigenin. The combination has a synergistic effect that can increase total flavonoid levels and the therapeutic effect of pilis. The method used in this research was litera-ture review and data analysis. The results showed the effect of transdermal patch pilis from the combined extracts added to the formula in vitro and in vivo using animal tests. In conclusion, transdermal patch pilis has prospects as a topical treatment based on the combination of an active delivery system with a patch and metered dose system.
THE EFFECT OF LONG SOAKING OF SEEDS IN KNO3 SOLUTION AND VARIATIONS IN PLANTING MEDIA ON THE GERMINATION OF CIPLUKAN (Physalis angulata L.) SEEDS IN VITRO hartati, Puji; Hastuti, Endah Dwi; Setiari, Nintya; Nurchayati, Yulita
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 11 No. 1 (2024)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2024.4968

Abstract

The Physalis angulata L. (ciplukan), originating from the Solanaceae family, contains secondary metabolites that can be used as medicinal materials. The provision of Physalis angulata seeds is hindered by seed dormancy. Dormancy can be broken by employing a 0.2% w/v KNO3 solution with a specific soaking period. This study aims to optimise the duration of the soaking period for Physalis angulata seed germination and the in vitro growing medium. The seeds were soaked in a 0.2% w/v KNO3 solution for 6 hours, 12 hours, and 24 hours. The soaked seeds were planted in sterile tissue media moistened with Murashige and Skoog (MS) medium solution, a 0.2% w/v KNO3 solution, and distilled water. Generally, the dormancy of Physalis angulata seeds can be broken by soaking them in a 0.2% w/v KNO3 solution. The results of this study indicated that Physalis angulata seeds soaked in a 0.2% w/v KNO3 solution for 6 hours and 12 hours, then planted in sterile tissue media moistened with the MS medium solution, exhibited an optimal germination response.
SARS-CoV-2 VARIANTS GENOME ANALYSIS OF INDONESIAN ISOLATES AND THEIR RESPONSES TO AVAILABLE VACCINES Audah, Kholis Abdurachim; Shaikho, Nasser Mohamed Ghassan Mohamed Adnan; Yurico, Dyana; Jeremy, Michael; Anisa, Amalda Siti
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 11 No. 1 (2024)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2024.5547

Abstract

SARS-CoV-2 is a virus that initially appeared in Wuhan, China, at the end of 2019. In Indonesia, multiple variants of SARS-CoV-2 have been identified, as well as various local variants that are not yet considered to be ‘variants of concern’. Therefore, this investigation is intended to understand the prevalence and epidemiology of the virus, along with detecting the mutations that occur in genes associated with whole-genome-sequences (WGS) isolated in Indonesia. Analyses were performed to investigate SARS-CoV-2 prevalence in Indonesia using data obtained from GISAID.org. Whole-genome sequencing was performed on random samples taken from GISAID.org utilizing the BLAST tool from NCBI. The variants identified in Indonesia are alpha, beta and delta variants, as well as local variants B.1.470 and B.1.466.2. In total there are 9,260 isolated genomes collected in GISAID were located in Indonesia. Using BLAST, the variants were compared with the Wild-Type from Wuhan NC.045512.2. Multiple mutations were observed in the samples. The results from whole-genome sequencing of variants isolated in Indonesia have found that multiple mutations have occurred in genes of the SARS-CoV-2 virus and it caused alterations in the characteristics of the virus and may affect vaccine efficacy.
OPTIMIZATION OF METHANE PRODUCTION FROM MIXED SUBSTRATES OF COW FAECES AND CARICA SEEDS USING RESPOND SURFACE METHODOLOGY Sutaryo, Sutaryo; Susanto, Setio; Purnomoadi, Agung; Abeng, Doni; Purwasih, Rita
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 11 No. 1 (2024)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2024.5554

Abstract

Anaerobic digestion (AD) of dairy cow feces (DCF) has low methane production per ton of waste. A strategy to overcome this drawback is to co-digest DCF and carica seed (CS). Currently, CS is still a waste from the candied carica in the syrup industry and is often just thrown away into the environment. This research aims to evaluate the optimal level of combination for methane production from DCF with co-substrates of germinated (CGM) and non-germinated (CNG) CS meal using Respond Surface Methodology (RSM). This research uses a completely randomized design with a factorial pattern consisting of the first factor being CGM and CNG, and the second factor being the combined level of DCF and CS (CGM or CNG. The result showed that utilization of CS can increase significantly (p<0.05) methane production of the final substrate compared to the control (digester treating DCF only). Utilization of CGM as co-substrate with DCF can also increase methane production (p<0.05) compared to CNG. All parameters in the liquid phase were in the normal range for AD. Based on the research results, the optimum point with a desirability value close to 1 was achieved at a ratio of CGM10 and DCF90. CS was proven can be used as a co-substrate with DCF to increase methane production of the final substrate and germination can be used as a method to increase the methane yield of CS. 
DIVERSITY AND DENSITY OF MEGABENTHOS IN CORAL REEF ECOSYSTEMS IN KERA ISLAND WATERS, KUPANG DISTRICT, INDONESIA Kangkan, Alexander L.; Sine, Kiik G.; Boik, Rini I.
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 11 No. 1 (2024)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2024.5597

Abstract

Kera Island belongs to the small islands of the East Nusa Tenggara with the potential conservation of coral reefs and various associated megabenthos organisms. This research aims to a) determine the diversity and density of megabenthos in coral reef ecosystems in Kera Island Waters, b) investigate the grouping between variables from environmental characteristics of megabenthos in coral reef ecosystems in Kera Island Waters, and c) observe the relationship between the diversity and density of megabenthos with the percentage of coral cover in Kera Island Waters. The research method used was a survey with direct measurements in the field. Megabenthos data was taken using the Benthos Belt Transect technique, while the coral cover was taken using the Underwater Photo Transect technique and analyzed using CPCe 4.1 software. The research data were then analyzed for density and percentage of megabenthos, diversity index value, (H’) Shannon, coral cover, organic matter, carbonate content, principal component analysis, and correlation. The study's results regarding the diversity index value of megabenthos in Kera Island Waters ranged from 0.796 to 1.631. The similarity of environmental parameters makes two main components. The first includes current velocity, organic content, dissolved oxygen, and depth; the second are carbonate content, TDS, temperature, and salinity characterize the second main component. The average organic matter was 8.32%, while the carbonate content was 50.09%. The coral reefs and megabenthos density had a robust relationship, with a correlation coefficient of 0.93.
TEMPERATURE EFFECTS ON THE STABILITY OF MYCOBACTERIUM TUBERCULO-SIS SHIKIMATE KINASE (MtSK): STRATEGIES FOR SECURE TRANSPORT Wibowo, Aji; Komariyah, Tinta; Prabandari, Erwahyuni Endang; Ariyani, Titin; Siska, Eka
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 11 No. 1 (2024)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2024.5751

Abstract

Mycobacterium tuberculosis Shikimate Kinase (MtSK) has a crucial role in the shikimic pathway, which is essential for this bacteria but is absent in humans, making it a potential target for novel anti-tuberculosis drugs. This study used enzyme-coupled fluorescence to examine the stability of MtSK stored in 50% glycerol at -30 ℃, 4 ℃, and ±28 ℃ for six days. Results showed stable enzyme activity values (α=0.05) at all temperatures. This research underscores that MtSK’s stability depends on its molecular properties, including GC content, hydrophobic residues, Mg2+ binding, and intra-helical salt bridge. Despite some activity decline over time due to glycerol-induced aggregation, MtSK can be safely transported at ±28 ℃ for up to six days without special cooling compartment. Understanding MtSK stability ensures its active conformation remains consistent, reducing off-target effects on drug design and enhancing drug efficacy. This insight ultimately leads to high-quality and commercially viable tuberculosis treatment development. Future research should explore MtSK stability at higher temperatures and assess the optimal glycerol content for cryopreservation.
COMBINED TREATMENT OF LANDFILL LEACHATE USING COAGULATION-FLOCCULATION AND ANAEROBIC-AEROBIC BIOFILTER WITH BIOBALL Sugito, Sugito; Ratnawati, Rhenny; Laba, Vinsensius Fereri
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 11 No. 2 (2024)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2024.5756

Abstract

Piles of waste in landfills can produce leachate that smells bad and contains organic and inorganic materials as well as some pathogenic bacteria. A biofilter is a simple method that can be used to degrade pollutant parameters dissolved in leachate. This research aims to determine the efficiency value of Biological Oxygen Demand (BOD), Chemical Oxygen Demand (COD), and Total Suspended Solid (TSS) concentrations in the leachate of Blandongan Landfill, Pasuruan City. The leachate treatment process uses the coagulation method as pretreatment with aluminum sulfate coagulants (Al2(SO4)3) and Ferric Chloride (FeCl3) followed by biofilter using bioball media. The variables in this research were the differences in the concentration of the coagulant used and the bioball media with a height of 15 cm. The results show that in bioball me-dia the value of reduction efficiency is better in reducing COD levels by 77.73%, BOD by 79.40%, and TSS by 81.48%. This research combines leachate treatment technol-ogy, where the coagulation-flocculation method becomes the pre-treatment of the leachate before continuing with an anaerobic-aerobic biofilter.
LEYDIG CELL COUNT IS INCREASES IN OLD WISTAR RATS (RATTUS NORVEGICUS) BY ANTIOXIDANT ETHANOL EXTRACT OF MORINGA OLEIFERA (EEMO) Widiastini, Luh Putu; Karuniadi, I Gusti Agung Manik
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 11 No. 1 (2024)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2024.5807

Abstract

Oxidative stress is the result of an imbalance between antioxidant production and the production of reactive oxygen species (ROS). Leydig cells have a high content of PUFAs in their cell membrane, making it easy to bind ROS. Most natural antioxidants are found in plants, including Moringa. In this work, aged Wistar rats' Leydig cells will be used to test the antioxidant activity of an ethanol extract from the leaves of Moringa oleivera (Rattus norvegicus). The study involved the division of 36 elderly rats, who were between the ages of 18 and 19 months, into two groups. For thirty days, the control group received the same quantity of 0.5% CMC every day, whereas the treatment group received 50 mg/kgBW/0.5 mL of Moringa leaf ethanol extract daily. The rats were healthy and free of physical impairments. The Independent Samples T-Test is used to assess the data in order to find any variations between the treatment and control groups. The significant difference (p < 0.00) in Leydig cell counts between the extract-treated group and the control group suggests that the ethanol extract of Moringa leaves can have a major effect on the number of Leydig cells in Old Wistar rats (Rattus Norvegicus).

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