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Proceedings of Annual International Conference Syiah Kuala University - Life Sciences & Engineering Chapter
Published by Universitas Syiah Kuala
ISSN : -     EISSN : -     DOI : -
Core Subject : Education, Engineering,
Engineering Other
Arjuna Subject : -
Articles 402 Documents
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Isolation and characterization of phage specific to E. coli O157:H7 Siti F.J. Zaman; Yahya Mat Arip
Proceedings of The Annual International Conference, Syiah Kuala University - Life Sciences & Engineering Chapter Vol 2, No 1 (2012): Life Sciences
Publisher : Syiah Kuala University

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Abstract

. Phage therapy is not new because it has been practiced since the early 1900s to cure diseases such as shigellosis, typhus and dysentery that caused by bacteria. However, when antibiotics were discovered in 1941, this antibacterial therapy was quickly abandoned by most western scientists. Antibiotic treatment is usually prescribed to treat pathogenic bacteria E. coliO157:H7. However, since 1950s, the widespread resistance to antibiotics has raised public concern on development of alternatives to antibiotics. As a result of this resistance, there becomes a need for an alternative treatment to work synergistically with antibiotic to deal with bacterial infections. This has led to a new approach by the use of bacteriophage which is phage therapy. Once again, this treatment has received attention as an alternative method against bacterial infection. Phage therapy is the treatment on pathogenic bacterial infections by using bacteriophage which capable of invading bacterial cells and disrupting bacterial metabolism before lysing it. Phages are extremely host-specific and the natural enemies of bacteria. It soon becomes clear that there are huge numbers of bacteriophages existed and waiting to be isolated. Phage specific to E. coliO157:H7 was successfully isolated from sewage treatment sample. The appearance of plaques on lawn of E. coliO157:H7 plate indicates phage capabilities to infect and lyses the host cells. The isolated phage was further characterized based on its morphology, genomic profile, physicochemical attributes, and host specificity to assess their potentials to be developed as phage therapy against E. coliO157:H7. 
Production and Physiological Characters of Soybean Varieties Under Drought Stress with Application of Nitrogen Sources Yaya Hasanah; Tengku Chairun Nisa; . Hapsoh; Hamidah Hanum
Proceedings of The Annual International Conference, Syiah Kuala University - Life Sciences & Engineering Chapter Vol 5, No 2 (2015): Life Sciences
Publisher : Syiah Kuala University

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Abstract

Soybean  is  a  legume  sensitive  to  drought  conditions  resulting  in  decreased  yield  and  seed quality.  Drought stress on plants also inhibits nitrogen uptake.  The objective of the research was to  determine production and physiological characters  of soybean varieties under drought stress through nutrient N management. This research used a randomized block design with  3 factors  and  3  replications.  The  first  factor  was  soybean  varieties  (Anjasmoro,  Wilis  and Sinabung). The second factor was drought stress conditions consisted of 50%, 60% , 70% and 80%   of field capacity (FC). The third factor was the application of N consisted of (1). Without application of N fertilizer (control);  (2). N fertilizer dose of recommendation (50 kg Urea/ha); (3). Inoculation of Bradyrhizobium sp. ; (4). Organic N sources (straw compost 10 tons/ha)  ; (5).  Organic  N  sources  (farmyard  manure  10  tons/ha).  The  results  suggest  that  Anjasmoro variety  improved dry weight of seed per plant compared with Willis and Sinabung.   Increased drought stress ( 80-50 % of FC) resulted in a decrease in dry weight of seed per plant. Sources  of  N  in  the  form  of  urea  or  Bradyrhizobium  sp.  increased  the  dry  weight  of  seeds  per  plant compared  with  treatment  N  sources  straw  and  manure  compost.  The  interaction  between Bradyrhizobium sp.  or Urea and Anjasmoro variety improved  dry  weight of seeds per plant
Effect of cavitation in cylindrical and two-dimensional nozzles on liquid jet formation Muhammad Ilham Maulana; . Jalaluddin
Proceedings of The Annual International Conference, Syiah Kuala University - Life Sciences & Engineering Chapter Vol 1, No 2 (2011): Engineering
Publisher : Syiah Kuala University

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Abstract

Cavitation in various nozzles of different geometries and dimensions, i.e., two-dimensional (2D) and cylindrical nozzles, and liquid jets discharged from the nozzles are visualized using a digital camera, and the index for estimating cavitation in a nozzle is discussed. Simultaneous high-speed visualization of cavitation in the cylindrical nozzle and a liquid jet are also carried out to investigate the relation between cavitation and ligament formation. As a result, the following conclusions are obtained: (1) Flow pattern transition in cavitating flows induces the transition in liquid jet patterns. As the liquid flow rate increases, flow patterns of cavitating flows and liquid jets transit from (no cavitation and wavy jet), (developing cavitation and wavy jet), (supercavitation and spray), to (hydraulic flip and flipping jet); (2) When the trace of a cavitation cloud comes out of the nozzle, a ligament is formed at the liquid jet interface. (3) The collapse of cavitation clouds near the exit and induces ligament formation, which, in turn, causes liquid jet atomization; (4) The causal relationship between cavitation cloud and ligament formation holds not only in the two-dimensional nozzle but also in the cylindrical nozzle
Students’ metacognitive skill in mathematics learning with cabri geometry II plus Rahmah Johar; . Khalisna; Taufik Fuadi Abidin
Proceedings of The Annual International Conference, Syiah Kuala University - Life Sciences & Engineering Chapter Vol 3, No 1 (2013): Life Sciences
Publisher : Syiah Kuala University

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Abstract

Metacognitive skill is the awareness of one's thinking about the thinkingprocess itself, whereas consciousness is the awareness of one's thinking about what to do. The subjects for this research were five students from class VII-1 SMP 1 Banda Aceh. Research data collection used the sheet metacognitive questions. Data were analyzed descriptively to interpret students' metacognitive skills in learning how to use Cabri Geometry II Plus software. The results showed that the metacognitive abilities of students increased from the first meeting to the fourth meeting
Influence of co-solvent on reactive-extraction of Jatropha curcas L. seed for biodiesel production M. Dani Supardan; . Satriana; Ryan Moulana
Proceedings of The Annual International Conference, Syiah Kuala University - Life Sciences & Engineering Chapter Vol 2, No 2 (2012): Engineering
Publisher : Syiah Kuala University

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Abstract

In this study, experimental studies have been carried out to improve the yield of biodiesel by addition of co-solvent to enhance the miscibility of the phases and speed up the reaction rate. The co-solvent used are tetrahydrofuran and hexane. The experimental result shows that the transesterification rate was improved when compared to the system without cosolvents. The biodiesel produced in the experiment was analyzed by gas chromatography-mass spectrometry (GC-MS), which showed that methyl oleate was the highest compound in biodiesel.
Sensitivity of Acehnese varieties of rice (Oryza sativa L.) to high temperature stress during flowering stage Sabaruddin Zakaria; . Bakhtiar; . Efendi
Proceedings of The Annual International Conference, Syiah Kuala University - Life Sciences & Engineering Chapter Vol 2, No 1 (2012): Life Sciences
Publisher : Syiah Kuala University

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Abstract

. Rice is very sensitive to high temperature stress at the flowering stage. The rice plants were exposed to high temperature condition under green house. Some varieties show tolerance to heat stress, and the others are sensitive. Nine local Acehness varieties: Si Puteh, Itam Tangke Lango, Ramos Tihion Tamping, Sireundeun Semantok, Bo Santeut Seumantok, Pade Cut Krusek, Sipirok, Sigeudop and Cantek Lembayung were observed for high temperature stress during flowering stage. The objective of this research are: to identify the effect of heat stress on reproductive stage of local Acehnese, Indonesian rice; and to find the varieties that sensitive and hopefully have the gene of “temperature genic male sterility” (TGMS) that can be used as restorer varieties for production of hybrid rice. Heat stress significantly decreased pollen viability, plant architecture, and yield components. The pollen viability was observed by stainability and germination rate of the pollen. Based on pollen germination ability showed that one variety of rice, Sigeudop, showed tolerance to high temperature stress. Whereas the sensitive variety was Bo Santeut Seumantok, the local Indonesian rice that is potential to have TGMS gene. Pollen stainability showed that all varieties of pollen involved stainability from plants cultivation at high temperature, and all the varieties had the high rate of pollen stainability. We also found that pollen germination rate showed three varieties of rice cultivated in high temperature involved high resistance of the pollens. 
Glycerolysis for Lowering Free FattyAcid of Waste Cooking Oil M. Dani Supardan; . Adisalamun; Yanti Meldasari; Yulia Annisa; . Mahlinda
Proceedings of The Annual International Conference, Syiah Kuala University - Life Sciences & Engineering Chapter Vol 5, No 1 (2015): Engineering
Publisher : Syiah Kuala University

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Abstract

Glycerolysis can be a useful alternative for lowering free fatty acid content present in the sustainable feedstock of waste cooking oil. In the present work, the effect of reaction parameters such as molar ratio of oil to glycerol (1:1, 1:2 and 1:3), catalyst NaOH concentration (0.875, 1.3 and 1.75 %-w of oil) and mass ratio of oil to co-solvent hexane (2:1, 4:1, 8:1) have been investigated. The reaction parameters have a remarkable effect on the free fatty acid (in term of acid value) of waste cooking oil. The final acid value decreased with an increased in molar ratio of oil to glycerol from 1:1 to 1:2. A significant reduction in acid value was not observed with a further increase in molar ratio of oil to glycerol. It has been observed that the optimum catalyst concentration of 1.3% can be reduced acid value from 12.2 to 1 mg KOH/g oil in 20 min. The addition of hexane as co-solvent affected the reaction rate due to immiscibility of the oil and glycerol phases. However, a suitable amount of co-solvent must be added to achieve an optimum of acid value reduction.
Water quality and water borne diseases in lowland ecosystem of Banyuasin, South Sumatra, Indonesia Dianita Ekawati; Tan Malaka; Robiyanto H. Susanto; M. T. Kamaluddin; Dwi Setyawan
Proceedings of The Annual International Conference, Syiah Kuala University - Life Sciences & Engineering Chapter Vol 1, No 1 (2011): Life Sciences
Publisher : Syiah Kuala University

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Abstract

Water quality and quantity is always an important issue in lowland ecosystem of Banyuasin. Low domestic water supply sanitation is considered as having an important contribution on the high frequency of waterborne diseases in the area. The study aims at recording water borne diseases and the water quality in the lowland area of Banyuasin District. This field research was conducted using a cross-sectional method. Total samples were 210 households in Telang which were observed during July through August 2011. Six water samples were measured for their physical and chemical properties, in addition to 1 water sample for bacteriological test. All respondents used river water for sanitary purposes and 41.4% of them also have dug wells. Those who used river water for sanitation, 68.5 % also consumed purified water for drinking purpose. All water samples are acidic (pH 5,68-6,19) and contain high Iron  (1-26,269 Mg/L). Water has yellowish color.  The major disease are diarrhea and skin diseases. In the lowland area with the estuarine ecosystem water quality and scarcity are serious problems. This in turn causes the high prevalent of waterborne diseases such as diarea. Low level of education and socio-economic status make it difficult for the local people to overcome these problems. Outside intervention especially government so as to improve the condition is needed
Morphology and physiology characteristic of some varities of rice under salinity stress Wan Arfiani Barus; Abdul Rauf; B. Sengli Damanik; . Rosmayati
Proceedings of The Annual International Conference, Syiah Kuala University - Life Sciences & Engineering Chapter Vol 3, No 1 (2013): Life Sciences
Publisher : Syiah Kuala University

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Abstract

This experiment was aimed to evaluate yield potential and adaptability of some varieties of rice based on some morphological and physiological characters. This study was conducted at Paluh Merbau village, Percut Sei Tuan Sub-District, Deli Serdang District, North Sumatera, Indonesia on March 2012 till July 2012. This research was arranged in a randomized block design non factorial with three replications. Eight varieties used are Ciherang, IR 64, Lambur, Batanghari, Banyuasin, IR 42, Inpara 10 and Margasari. Salinity stress in rice leads to changes in both morphological and physiological characters of plants, where the response of each genotype varies depending on the nature of tolerance or sensitivity to salt stress. Observation of growth and production showed that genotif IR 42 had the best growth and the highest production as compared with other varieties
Studies on antibiotic compounds of methanol extract of Curculigo latifolia dryand Lim Sheh Hong; Darah Ibrahim
Proceedings of The Annual International Conference, Syiah Kuala University - Life Sciences & Engineering Chapter Vol 2, No 1 (2012): Life Sciences
Publisher : Syiah Kuala University

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Abstract

A research was conducted to study the effect of different parts of the extracts from Curculigo latifolia Dryand plant.  They were from the roots, stems and leaves by using methanol as the extraction solvent.  The antimicrobial activity of methanol extract from Curculigo latifolia Dryand was then performed on various tested bacteria, yeasts and fungi.  The results revealed that the extracts possessed antimicrobial activities on all of tested bacteria and yeasts. However, all the tested fungi exhibited resistant against all the different parts of Curculigo latifolia Dryand extracts. The minimum inhibitory concentration (MIC) and minimum lethality concentration (MLC) of the Curculigo latifolia Dryand extracts against bacteria and yeast cells were determined and the mode of action of the roots extract on the cells was studied by means of microscopy

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