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Contact Name
Trio Ageng Prayitno
Contact Email
trioagengprayitno@uibu.ac.id
Phone
+6285746890990
Journal Mail Official
edubiotik@uibu.ac.id
Editorial Address
Jl. Citandui No.46, Purwantoro, Kec. Blimbing, Kota Malang, Jawa Timur 65126, Email: edubiotik@uibu.ac.id
Location
Kota malang,
Jawa timur
INDONESIA
Edubiotik : Jurnal Pendidikan, Biologi dan Terapan
ISSN : 2528679X     EISSN : 25979833     DOI : https://doi.org/10.33503/ebio.v9i02
Edubiotik: Jurnal Pendidikan, Biologi dan Terapan aims to bridge the gap between research and practice, providing information, ideas, and opinions in biology research, teaching, and learning. Through coverage of policy and curriculum developments, the latest research results on biology teaching, learning, and assessment are brought to the surface. Edubiotik: Jurnal Pendidikan, Biologi dan Terapan accepts and publishes articles in the form of research in the fields of biology education, pure biology, and applied biology. All articles are published in English and undergo a peer-review process. The scope of Edubiotik: Jurnal Pendidikan, Biologi dan Terapan is focused on research in biology education, pure biology, and applied biology both in the topics discussed and the perspective of the discipline: 1. Biology teaching and learning materials at all levels of education; 2. Pure biology research developed or studied on sources, materials, or biology learning media (using the research and development/R&D paradigm); 3. Biology education curriculum at all levels of education; 4. Quasi-experiments, classroom action research (PTK), and Lesson Study in biology learning. Other qualitative research in biology education; 5. Evaluation/assessment of biology learning; 6. Professional issues/trends of teachers in biology education; 7. Environmental education; 8. All topics in applied biology.
Articles 182 Documents
Characterization of soil pathogenic fungi in the rhizosphere of siwalan palm (Borassus flabellifer L.) Nurtjahyani, Supiana Dian; Sulistyaningrum, Heny; Sulistiono; Amin, Mochamad; Tegar Kurnia, Dwi; Dhiaulhaq, M. Rafi; Mustofa, Ali
Edubiotik : Jurnal Pendidikan, Biologi dan Terapan Vol. 10 No. 02 (2025): Edubiotik : Jurnal Pendidikan, Biologi dan Terapan
Publisher : Biology Education Department, Universitas Insan Budi Utomo, Malang, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33503/ebio.v10i02.2156

Abstract

Soil-borne fungal pathogens represent a persistent threat to plant health due to their ability to colonize the rhizosphere and infect root systems, yet information on their diversity in non-industrial palm species remains limited. Siwalan (Borassus flabellifer L.) is a drought-tolerant palm of ecological and socio-economic importance, but its rhizospheric fungal communities have not been systematically characterized. This study aimed to (1) isolate soil-borne fungi from the rhizosphere of siwalan and (2) identify potential pathogenic fungi based on macroscopic and microscopic morphological characteristics. Rhizosphere soil samples were collected at depths of 5–20 cm and processed using the soil dilution plate method. Fungal isolates were cultured on potato dextrose agar (PDA), purified through subculturing, and identified using colony morphology and microscopic structures observed through slide culture and lactophenol cotton blue staining. Data were analyzed descriptively by comparing fungal traits with standard taxonomic determination keys. Five soil-borne fungal taxa were successfully isolated and identified, namely Aspergillus flavus, Trichoderma longibrachiatum, Aspergillus oryzae, Aspergillus caelatus, and Aspergillus niger. The isolates exhibited distinct differences in colony pigmentation, growth rate, hyphal structure, vesicle morphology, and conidial characteristics. Among them, A. niger showed the fastest radial growth and highest sporulation intensity, whereas T. longibrachiatum and A. oryzae exhibited relatively slower growth. These morphological variations indicate functional diversity and differing adaptive strategies among fungi inhabiting the siwalan rhizosphere. This study provides the first systematic morphological baseline of soil-borne fungi associated with the rhizosphere of B. flabellifer. The findings contribute novel information on fungal diversity in an underexplored palm species and highlight the importance of early fungal characterization as a foundation for future molecular identification, pathogenicity testing, and the development of sustainable disease management strategies for siwalan cultivation systems.
Phage cocktails as a new antibiotic for combating bacteria consortium in foodborne disease Marzuqi, Salsabila Jahroh; Putri, Adelia Meita; Purwanto, Martinus Effand Pratama; Murtadho, Ahmad Hibban; Grahita, Amanda Claudia Ayuning; Narulita, Erlia
Edubiotik : Jurnal Pendidikan, Biologi dan Terapan Vol. 10 No. 02 (2025): Edubiotik : Jurnal Pendidikan, Biologi dan Terapan
Publisher : Biology Education Department, Universitas Insan Budi Utomo, Malang, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33503/ebio.v10i02.2394

Abstract

Foodborne diseases pose a critical global health threat, exacerbated by the alarming rise of multidrug-resistant (MDR) bacterial strains like Staphylococcus aureus, Escherichia coli, and Salmonella typhi. Conventional antibiotics are increasingly ineffective, necessitating the development of alternative therapeutic and biocontrol strategies. This study aims to evaluate the efficacy and stability of a bacteriophage cocktail as a potential non-antibiotic alternative for controlling a consortium of foodborne pathogenic bacteria. The research employed an experimental design using revived cultures of the three bacterial species and three phage isolates (ϕSZUT-01, ϕSZIP-01, ϕSZIP-02) as research samples. Data collected included bacterial growth kinetics, observation of lysis zones, and phage stability at various pH levels and storage temperatures. Data analysis was conducted descriptively, followed by one-way ANOVA at a 95% confidence level to determine differences among treatments. Three two-phage cocktails were formulated and tested, with all treatments consistently demonstrating strong lytic activity, evidenced by large and clear plaques. Lysis kinetics, monitored through OD600 measurements, confirmed rapid bacterial elimination beginning approximately 180 minutes post-infection. Notably, the bacterial consortium exhibited resistance to the positive control (Chloramphenicol), reinforcing the need for alternative interventions. Additionally, the phage cocktail demonstrated excellent stability, maintaining infectious titers across a broad pH range and during storage at both refrigerated (4°C) and room temperatures (21°C). This high stability supports its potential applicability in food safety systems and resilience in gastrointestinal environments. Overall, the bacteriophage cocktail demonstrated effective antibacterial activity against a consortium of foodborne pathogenic bacteria, as evidenced by the formation of clear lysis zones and exhibited high stability, supporting its potential application as a non-antibiotic alternative for controlling foodborne diseases.