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Contact Name
Trio Ageng Prayitno
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Jl. Citandui No.46, Purwantoro, Kec. Blimbing, Kota Malang, Jawa Timur 65126, Email: edubiotik@uibu.ac.id
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INDONESIA
Edubiotik : Jurnal Pendidikan, Biologi dan Terapan
ISSN : 2528679X     EISSN : 25979833     DOI : https://doi.org/10.33503/ebio.v9i02
Edubiotik: Jurnal Pendidikan, Biologi dan Terapan aims to bridge the gap between research and practice, providing information, ideas, and opinions in biology research, teaching, and learning. Through coverage of policy and curriculum developments, the latest research results on biology teaching, learning, and assessment are brought to the surface. Edubiotik: Jurnal Pendidikan, Biologi dan Terapan accepts and publishes articles in the form of research in the fields of biology education, pure biology, and applied biology. All articles are published in English and undergo a peer-review process. The scope of Edubiotik: Jurnal Pendidikan, Biologi dan Terapan is focused on research in biology education, pure biology, and applied biology both in the topics discussed and the perspective of the discipline: 1. Biology teaching and learning materials at all levels of education; 2. Pure biology research developed or studied on sources, materials, or biology learning media (using the research and development/R&D paradigm); 3. Biology education curriculum at all levels of education; 4. Quasi-experiments, classroom action research (PTK), and Lesson Study in biology learning. Other qualitative research in biology education; 5. Evaluation/assessment of biology learning; 6. Professional issues/trends of teachers in biology education; 7. Environmental education; 8. All topics in applied biology.
Articles 182 Documents
The influence of variative learning models on the critical thinking skills of multiethnic state junior high school students in Ternate City Ermin; Arifin , As’ad Syamsul
Edubiotik : Jurnal Pendidikan, Biologi dan Terapan Vol. 10 No. 02 (2025): Edubiotik : Jurnal Pendidikan, Biologi dan Terapan
Publisher : Biology Education Department, Universitas Insan Budi Utomo, Malang, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33503/ebio.v10i02.1605

Abstract

Critical thinking is an essential skill that enables students to analyze, evaluate, and solve problems rationally and reflectively. However, learning practices show that students’ critical thinking skills remain relatively low, indicating the need for more effective instructional strategies. This study aimed to examine the effects of the STAD model, the NHT model, and a combination of STAD and NHT on the critical thinking skills of multiethnic junior high school students in Ternate City. This study employed a quasi-experimental method using a pretest–posttest nonequivalent control group design. The population consisted of all Grade VII multiethnic students in Ternate City junior high schools, with a sample of 76 students comprising 20 Ternate, 27 Makian, and 29 Tidore students. Research instruments included observation sheets and critical thinking skill tests. Data were collected through pretests and posttests and analyzed using descriptive statistics and inferential statistics with analysis of covariance (ANCOVA) at a 0.5% significance level.The results showed that learning models significantly affected students’ critical thinking skills. The STAD model combined with NHT resulted in higher critical thinking skills than STAD, NHT, and conventional learning. Ethnicity also had a significant effect, with Ternate students demonstrating higher critical thinking skills than Makian and Tidore students. Furthermore, the interaction between learning strategies and ethnicity significantly influenced students’ critical thinking skills. Overall, the combination of STAD and NHT was found to be effective in improving the critical thinking skills of students from all ethnic groups.
The ethnobotany study of Babad Dalan ritual ceremony (BDRC) of Javanese in Gunungkidul, Yogyakarta to biocultural conservation Silalahi, Marina; Wahyuningtyas, Riska Septia; Kalima , Titi; Situmorang , Agnes Feronika
Edubiotik : Jurnal Pendidikan, Biologi dan Terapan Vol. 10 No. 02 (2025): Edubiotik : Jurnal Pendidikan, Biologi dan Terapan
Publisher : Biology Education Department, Universitas Insan Budi Utomo, Malang, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33503/ebio.v10i02.1620

Abstract

The Babad Dalan ritual ceremony (BDRC) is a ritual ceremony by the local Javanese ethnic community in Gunungkidul which has been carried out for hundreds of years using various types of plants. Biocultural studies of ritual ceremonies as part of biodiversity and cultural conservation are still limited. The purpose of this study is to explain the process of carrying out the ritual and explain the diversity of plants used in BDRC. This study was conducted using an ethnobotanical approach through surveys and semi-structured interviews. Respondents consisted of key informants (5 people) and general respondents (25 people) who were determined by purposive sampling. Data analysis was carried out qualitatively using descriptive statistics. Results: The local Javanese ethnic community in Gunungkidul carries out BDRC every year as a ceremony of gratitude to God for the success of an abundant harvest. A total of fifty-eight species belonging to fifty-one genera and twenty-seven families are used in BDRC which are used as ingredients for offerings, gunungan, and tumpeng. Plants are used as components of gunungan "mountains" (22 species), offerings (12 species), gudangan/traditional cuisine (8 species) and spices (10 species). Local communities cultivate various types of plants to ensure availability in the BDRC as part of plant biodiversity conservation. Conclusion: The use of plants in BDRC is local wisdom which directly or indirectly has implications for the preservation of bio-culture and biodiversity.
Construction and optimization of human (Homo sapiens) mitochondrial DNA primers in the D-loop hypervariable segment II region in forensic biology Nitisara , Antonia Anindyanari Paramartastri; Saamia, Vira; Purba, Laurentia Henrieta Permita Sari
Edubiotik : Jurnal Pendidikan, Biologi dan Terapan Vol. 10 No. 02 (2025): Edubiotik : Jurnal Pendidikan, Biologi dan Terapan
Publisher : Biology Education Department, Universitas Insan Budi Utomo, Malang, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33503/ebio.v10i02.1657

Abstract

DNA analysis is fundamental in forensic identification; however, nuclear DNA profiling often fails when samples are degraded. Mitochondrial DNA (mtDNA), which is maternally inherited and present in high copy numbers, provides a reliable alternative, particularly through analysis of the D-loop hypervariable segment II (HVS II). This study aimed to design and optimize human mtDNA HVS II–specific primers for forensic applications.An experimental approach combining in silico and in vitro methods was employed. Primer design and evaluation were conducted using the NCBI database, Primer3Plus, and NetPrimer Biosoft. Buccal swab samples from seven individuals were extracted using the PrepFiler™ Forensic DNA Extraction Kit, and DNA quality was assessed using a NanoVue spectrophotometer. Primer optimization was performed using gradient PCR, and sequencing was carried out using the Sanger method.Two primer pairs successfully amplified the HVS II region, generating fragments of 433–513 bp at an optimal annealing temperature of 56.5 °C. Sequence analysis revealed heteroplasmy and identified haplogroups N21 and L4b within the same maternal lineage. Both in silico and in vitro results confirmed that the designed primers specifically and reliably amplified human mtDNA.In conclusion, the optimized HVS II primers demonstrate strong potential for forensic casework involving degraded or limited biological samples and support improved resolution in population-level mtDNA analyses.
In silico analysis of bioactive compounds from Strobilanthes crispus as MurE inhibitors in Escherichia coli Aria Aditama , Yoga; Rakhmawati Afrida , Indah; Munandar, Kukuh; Riyanto
Edubiotik : Jurnal Pendidikan, Biologi dan Terapan Vol. 10 No. 02 (2025): Edubiotik : Jurnal Pendidikan, Biologi dan Terapan
Publisher : Biology Education Department, Universitas Insan Budi Utomo, Malang, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33503/ebio.v10i02.1721

Abstract

Urinary Tract Infection (UTI) is one of the most common infectious diseases, predominantly caused by Escherichia coli. Increasing antibiotic resistance has driven the search for new antibacterial agents from natural sources. This study aims to evaluate the potential of bioactive compounds from Strobilanthes crispus as inhibitors of the E. coli MurE enzyme using an in-silico approach. Molecular docking, toxicity prediction, and pharmacokinetic analysis were performed. The docking results demonstrated that apigenin 7-O-beta-D-glucuronide exhibited the highest binding affinity toward MurE with a binding energy of -9,5 kcal/mol, followed by acteoside (-9,4 kcal/mol) and isoacteoside (-9,2 kcal/mol), outperforming ciprofloxacin (-7,1 kcal/mol). Pharmacokinetic analysis indicated that all tested compounds showed good solubility and acceptable safety profiles, althought they exhibited los gastrointestinal absorption and poor oral bioavailability. These findings suggest that S. crispus bioactive compounds possess promising potential as MurE inhibitors and warrant further optimization and experimental validation as antibacterial candidates against E. coli.
Influence of vegetation structure on the diversity and distribution of nocturnal insects in oil palm plantations of Central Kalimantan Savitri, Shanty; Fajri, Samsul; Araina, Elga; Basuki, Bejo; Haryono, Agus; Fahrina, Ririn; Fatiqin, Awalul; Kioumarsi, Hamed
Edubiotik : Jurnal Pendidikan, Biologi dan Terapan Vol. 10 No. 02 (2025): Edubiotik : Jurnal Pendidikan, Biologi dan Terapan
Publisher : Biology Education Department, Universitas Insan Budi Utomo, Malang, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33503/ebio.v10i02.1770

Abstract

Environmental changes caused by oil palm plantation activities can affect the diversity of nocturnal insects that play important roles in the ecosystem as pollinators, decomposers, predators, and food sources for other insects as well as bioindicators. This study aimed to determine the levels of diversity, evenness, and dominance of nocturnal insects at two stations with different oil palm planting ages in Seruyan Regency. This study used a descriptive quantitative approach with an exploratory survey method. Nocturnal insects were collected from replanting and non-replanting oil palm areas using light traps equipped with UV lamps. The insects were identified to the species level, and data were analyzed using the Shannon–Wiener diversity index (H′), Pielou’s evenness index (E), and Simpson’s dominance index (C) with the PAST software. The results showed that from a total of 454 individuals, 15 species of nocturnal insects were successfully identified, originating from 6 orders, namely, Coleoptera (Laccophilus gentilis, Protaetia acuminata, Pangaeus bilineatus, Adoretus versutus, Denticollis linearis, Eumorphus bulbosus, Sirthenea flavipes, Cicindela gallica), Hymenoptera (Vespa affinis, Tetraponera rufonigra), Hemiptera (Pangaeus bilineatus), Odonata (Agriocnemis pygmaea), Lepidoptera (Hypochrosis subrufa, Chytolita morbidalis), and Orthoptera (Gryllus assimilis). The most dominant species was Pangaeus bilineatus with 143 individuals, while the species with the lowest number of individuals was Cicindela gallica with 3 individuals. The Shannon‒Wiener diversity index (H') value of 1.558 (Station I) and 2.129 (Station II) indicates moderate diversity, the Pielou evenness index (E) value of 0.59 (Station I) and 0.65 (Station II) indicates a fairly even distribution, and the Simpson dominance index (C) value of 0.72 (Station I) and 0.85 (Station II) indicates no extreme species dominance. The study revealed moderate nocturnal insect diversity with balanced species distribution, indicating a stable oil palm ecosystem and supporting their role as bioindicators of ecological health.
Vegetation analysis in Gembes spring area, Ngrayun, Ponorogo regency Ma’rufah, Amirul; Kusuma Dewi , Nurul; Sulistyarsi, Ani
Edubiotik : Jurnal Pendidikan, Biologi dan Terapan Vol. 10 No. 02 (2025): Edubiotik : Jurnal Pendidikan, Biologi dan Terapan
Publisher : Biology Education Department, Universitas Insan Budi Utomo, Malang, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33503/ebio.v10i02.1796

Abstract

Vegetation plays a vital role in water conversion, and one location that provides clean water is a spring. A spring is a location where water emerges from the ground surface. This study aims to collect data on vegetation around Gembes Spring. This type of research is quantitative, using a census research method for tree vegetation and a quadrat plot for understory vegetation. The population used in this research consists of all plants that comprise the vegetation in the spring area. The required samples are plant species found in the observation plot and those identified through direct observation of the trees. Research results have shown a very high level of diversity. The understory vegetation species that dominate the spring area are herbaceous growth forms, while the tree vegetation is dominated by the species Syzygium densiflorum. In conclusion, Zingiber spectabile. The species has the highest INP, followed by Ageratina riparia, which has the second-highest INP, and Globba marantina, with the third-highest INP among all the plant species that make up the understory vegetation.
The diversity of macroinvertebrates as a bioindicator of water quality in the Amprong River, Kedungkandang District Dwi Oktavia, Rizky; Wijayanti, Titik; Arifin, As'ad Syamsul; Hindun, Nurmala
Edubiotik : Jurnal Pendidikan, Biologi dan Terapan Vol. 10 No. 02 (2025): Edubiotik : Jurnal Pendidikan, Biologi dan Terapan
Publisher : Biology Education Department, Universitas Insan Budi Utomo, Malang, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33503/ebio.v10i02.2013

Abstract

The Amprong River in Kedungkandang District, Malang City, is facing ecological pressure due to dense residential settlements and inadequate domestic waste management. This study was conducted to assess the diversity of macroinvertebrates and to investigate their role as indicators of water quality. A quantitative descriptive method was employed, with samples collected from three stations (upstream, midstream, and downstream) on both sides of the river. Temperature and pH were recorded as key environmental parameters, and the structure of the macroinvertebrate community was analyzed using the Shannon–Wiener diversity index (H'), the evenness index (E), and Simpson’s dominance index (C). A total of 633 individuals representing 13 species and 8 families were identified. The results revealed moderate diversity in most sampling points, while one site exhibited low diversity and high dominance by Melanoides plicarius, indicating heavy pollution. Variations in species composition and ecological indices between riverbanks were influenced by habitat differences, namely vegetation type, substrate, and waste exposure. Overall, the results suggest that the Amprong River is classified between moderately and heavily polluted, underscoring the importance of enhanced environmental management and adequate waste treatment facilities to support river conservation in urban settings.
Bird diversity in the Mount Dempo area, South Sumatra: An ecological resource for local-based biology learning Armanda, Fahmy; Wicaksono, Anggun; Oktiansyah, Rian
Edubiotik : Jurnal Pendidikan, Biologi dan Terapan Vol. 10 No. 02 (2025): Edubiotik : Jurnal Pendidikan, Biologi dan Terapan
Publisher : Biology Education Department, Universitas Insan Budi Utomo, Malang, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33503/ebio.v10i02.2031

Abstract

Mount Dempo, South Sumatra, hosted a diverse range of bird species, making it a valuable ecological resource for local-based biology learning. This study aimed to document bird diversity in the region and to explore its potential for ecological education. The research was conducted in 2020 during the COVID-19 pandemic era. Data collection was carried out in the Mount Dempo area, limited to an altitude of approximately 2,086 meters above sea level at Shelter 1. The research employed the observation (point count) method along ecotourism routes by recording trails traversed using GPS. Sampling was conducted by walking along the path accompanied by predetermined observation points. The results recorded 21 bird species with a total of 96 individuals. The diversity analysis using the Shannon-Wiener Index produced a value of H' = 2.68, indicating moderate to high diversity, while the Simpson’s Index (D = 0.092) suggested very low species dominance. Additionally, the Evenness Index (E = 0.88) reflected a relatively even distribution of individuals among species, highlighting ecological stability in the study area. The presence of various ecological niches and bird functional groups supported the development of environmental literacy and conservation awareness among students. Thus, integrating local bird diversity as a contextual learning resource was considered a strategic approach to enhance ecological understanding and foster pro-environmental behavior among learners.
Enhancing students’ critical thinking skills: Developing a biology practicum module using the PjBL model by integrating local Banana Goroho Rengkuan , Mariana; Howan , Dian Herlinda Octorina; Leasa, Marleny
Edubiotik : Jurnal Pendidikan, Biologi dan Terapan Vol. 10 No. 02 (2025): Edubiotik : Jurnal Pendidikan, Biologi dan Terapan
Publisher : Biology Education Department, Universitas Insan Budi Utomo, Malang, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33503/ebio.v10i02.2154

Abstract

The lack of integration of project-based learning (PjBL) in nutrition education, especially in the context of local foods that have great potential but have not been widely explored, especially the use of (Musa acuminafe) as a practicum material, which is an alternative carbohydrate source typical of the Minahasa region. This study aims to develop a practicum module that enhances thinking skills using the PjBL model by testing the nutritional content of local foods of the Minahasa community (local banana Goroho/Musa acuminafe). This study employed a research and development (R&D) approach using the ASIE model (Analyse, Design, Implement, and Evaluate). The research subjects were undergraduate Biology students at Manado State University, with a sample of 30 students enrolled in the first semester of the 2024/2025 academic year. The research instruments included module validation sheets, student response questionnaires, and critical thinking skills tests to assess the effectiveness of the developed practicum module with the N-Gain score. The validation results from material and media experts show that the developed module is very valid, with scores reaching 96.166% and 95.6%, respectively. Furthermore, testing of students' critical thinking skills showed very good results, with an average score reaching 85.51%. In addition, student responses to this practicum module were also very positive, with an average score of 91.074%, which indicates a very practical category. These findings confirm the module's effectiveness in enhancing students' critical thinking skills (N-Gain=0.71, high category) and its relevance in the context of locally-based nutrition education. This research significantly contributes to the development of innovative and community-relevant learning methods and can serve as a reference for further research in education and nutrition.
GrowLab: The effect of augmented reality-based learning on secondary school students’ critical thinking skills in plant structure instruction Lidiastuti, Arinda Eka; Priyanti, Novie Ary; Arsyad, M.; Shobirin, Achmad
Edubiotik : Jurnal Pendidikan, Biologi dan Terapan Vol. 10 No. 02 (2025): Edubiotik : Jurnal Pendidikan, Biologi dan Terapan
Publisher : Biology Education Department, Universitas Insan Budi Utomo, Malang, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33503/ebio.v10i02.2155

Abstract

Plant growth is fundamental to understanding biological systems, particularly at the secondary school level. Learning about plant structure is a crucial component of biology education; however, many students find this topic challenging because it is often perceived as abstract. Augmented Reality (AR) technology offers an opportunity to enhance learning experiences by enabling interactive three-dimensional visualizations of plant structures. This study investigates the effect of AR-based GrowLab learning on students’ critical thinking skills in the context of plant structure instruction. A quasi-experimental design was employed involving 142 Grade XII secondary school students in Malang. Critical thinking skills were assessed using essay-based test instruments. Data were analyzed using an ANCOVA test with SPSS version 29. The results indicated that AR-based GrowLab learning had a significant effect on students’ critical thinking skills in teaching plant structure (sig < 0.05). The study concludes that AR positively affected learning, particularly in understanding plant growth and structure. Furthermore, the findings suggest that augmented reality should be integrated with pedagogical approaches such as problem-based learning (PBL) to maximize its effectiveness in enhancing students’ critical thinking skills.