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Nani Kurnia
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Bionature
Published by Universitas Negeri Makassar
ISSN : 14114720     EISSN : 26545160     DOI : 10.35580
Core Subject : Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology
Bionature is a journal that contains writings based on the results of research, conceptual ideas, studies, and the implementation of the theory in the field of biology.
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ecology, Evolution, Behavior and Systematics
Articles 90 Documents
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Daya Terima dan Kandungan Gizi Kue Bolu Cukke Subtitusi Dedak Padi Andi Bida Purnamasari; Putri Athira Azis
Bionature Vol. 23 No. 2 (2022): Oktober 2022
Publisher : Jurusan Biologi Universitas Negeri Makassar

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Abstract

This study aims to determine the acceptability and nutritional content of sponge cake with rice bran substitution. This research was carried out at the Biology Laboratory of STKIP Pembangunan Indonesia in 2019. This research began with rice bran sifting followed by making sponge cake, panelist assessment, analysis of nutritional content, data processing and analysis and finally the preparation of research reports. Protein content analysis using the Kjeldahl method. Analysis of fat content using the gravimetric method. Analysis of carbohydrate content using the titrimetric method. Receiving power data were analyzed using frequency distribution. The level of difference in acceptability was analyzed using the Kruskal-Wallis test. The results of this study indicate that the acceptability based on the taste aspect in the three groups is relatively the same (p = 0.275) but based on the Mean Ranks, the 25% sample group is preferred by the panelists. There are color differences in the four sample groups (p=0.000), the 25% sample group is preferred by the panelists. There were differences in aroma in the three sample groups (p=0.013), the 25% sample group was preferred by the panelists. The acceptability based on the taste aspect in the three sample groups was relatively the same (p=0.733) but based on the Mean Ranks, the 25% sample group was preferred by the panelists. The highest fat content is found in 25% rice bran substitution, the highest proteincontent is in 25% rice bran substitution, while the highest carbohydrate content is in 50% rice bran substitution.
Studi Tuberkulosis Kontak Serumah pada Masyarakat Pesisir di Wilayah Kerja Puskesmas Siko Kota Ternate Erpi Nurdin; Irma B. Hi. Lewa; Mukhtasyam Zuchrullah
Bionature Vol. 23 No. 2 (2022): Oktober 2022
Publisher : Jurusan Biologi Universitas Negeri Makassar

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Abstract

Pulmonary tuberculosis is still the main cause of death in the world, accounting for 2 million people die every year. WHO states that around 1.9 billion people, one third of the world's population, have been infected with tuberculosis germs, every second one person is infected with tuberculosis in the world. Pulmonary tuberculosis is transmitted through droplet spread with the source of infection being patients who are coughing with household contacts as a high risk group for transmission. To find out the results of the examination and see the percentage of positive or negative acid-fast bacilli (AFB) in a sample of clients who have a history of household contact with patients with pulmonary tuberculosis. This study uses a with correlation approach research method with an observational approach. The sampling technique is purposive sampling, namely sampling based on the considerations of the researcher. The examination method used was the Zielh Nelseen method of 30 sputum samples examined. From 30 respondents, 4 respondents were found positive for microscopic acid-fast bacilli (AFB). 2 positive family members, have the characteristics of dense housing (not eligible), humidity and ventilation do not meet the requirements, but the type of floor still meets the requirements. Based on the research that has been carried out, it is concluded that 4 (13%) respondents were identified as positive for scanty with the presence of tuberculosis in 3 places of tuberculosis in household contacts, with factors that influence the occurrence of tuberculosis in household contacts. There is no significant relationship between the results of AFB examination and gender (p value = 0.407> 0.05). There is no significant relationship between the results of AFB examination and age (p value = 0.272> 0.05).
Identification of Bacteria on Housefly Exoskeletons (Musca domestica) collected in Universitas Negeri Makassar Audriansyah, Lulu Ulfah; Daud, Firdaus; Syamsiah
Bionature Vol. 26 No. 2 (2025): Oktober-Desember 2025
Publisher : Jurusan Biologi Universitas Negeri Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35580/bionature.v26i2.6560

Abstract

The housefly (Musca domestica) is a vector that can spread bacteria through its body organs. This study aims to identify the bacteria on the exoskeleton of houseflies. The samples were collected from boarding houses, Biology department and postgraduate program of Universitas Negeri Makassar using insect nets. Morphological observations were conducted on selective media (EMBA, MSA, and TCBSA), followed by Gram staining and biochemical tests. The results revealed six types of bacteria: Enterobacter sp., Escherichia coli, Staphylococcus aureus, Staphylococcus epidermidis, Vibrio cho­­lerae, and Vibrio parahaemolyticus. Escherichia coli was found on houseflies collected from boarding houses. Staphylococcus aureus, Staphylococcus epidermidis, and Escherichia coli were identified on houseflies from the postgraduate campus. Meanwhile, Enterobacter sp., Staphylococcus aureus, Vibrio cholerae, and Vibrio parahaemolyticus were found on houseflies from the Biology department campus. These findings indicate that houseflies from the Biology department campus harbored a greater quantity and diversity of bacteria compared to samples from other locations.
Ethnobotanical Study Ethnobotanical Study and Phytochemical Review of Medicinal Plants Used by the Pandalungan Community in Taman Village, Bondowoso, Indonesia: An Insight into Traditional Knowledge and Bioactive Potential for Sustainable Utilization Nia, Imaniah Bazlina Wardani; Adzkafillah Nuruzzaman; Risma Nurlim; Nanda Eska Anugrah Nasution; Wahyu Anggar Wanto
Bionature Vol. 26 No. 2 (2025): Oktober-Desember 2025
Publisher : Jurusan Biologi Universitas Negeri Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35580/bionature.v26i2.9416

Abstract

Indonesia, as a megadiverse country, holds extensive biodiversity and rich local knowledge, particularly in the use of medicinal plants. This study aimed to document the use of traditional medicinal plants by the Pandalungan community in Taman Village, Grujugan District, Bondowoso Regency, and to assess their phytochemical potential. A qualitative approach was employed through semi-structured interviews using purposive and snowball sampling. Data was collected covering plant species, parts used, and sources of acquisition. A literature-based phytochemical review was also conducted to evaluate the scientific potential of the identified species. The Use Value (UV) was calculated to determine the cultural importance of each species. A total of 25 medicinal plant species were documented, with 13 cultivated, 5 wild, and 7 sourced from traditional markets. The highest UV scores were recorded for Zingiber officinale, Piper betle, Moringa oleifera, and Syzygium polyanthum. Literature analysis revealed that these plants contain key phytochemicals such as flavonoids, alkaloids, saponins, tannins, terpenoids, phenols, and essential oils. The findings indicate that local ethnobotanical knowledge holds significant potential for developing natural remedies based on local biodiversity, reinforcing the need for further research and conservation efforts.
Sintesis Nanopartikel Ekstrak Kulit Batang Tanaman Kayu Jawa (Lannea Coromandelica) Tersalut Kitosan Hartati; Fitratul Insaniah Rusli; Andi Mu'nisa; Halifah Pagarra
Bionature Vol. 23 No. 2 (2022): Oktober 2022
Publisher : Jurusan Biologi Universitas Negeri Makassar

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Abstract

This study aims to synthesize nanoparticles of Javanese bark extract (Lannea coromandelica) coated with chitosan and characterization of the synthesis results. The extraction of Javanese bark (Lannea coromandelica) was carried out by the maceration method using a 70% ethanol solvent. The bark extract of the Javanese wood plant (Lannea coromandelica) was synthesized to form chitosan-coated nanoparticles using the ionic glassing method and the synthesis results were characterized using X-Ray Difraction (XRD). The results showed that the synthesis of nanoparticles of the bark extract of the Javanese wood plant (Lannea coromandelica) coated in chitosan has a nanoparticle size of 24.9 nm.
Karakterisasi Isolat-isolat Beauveria Bassiana Galur Lokal Sulawesi Selatan Rachmawaty
Bionature Vol. 9 No. 2 (2008): 2008
Publisher : Jurusan Biologi Universitas Negeri Makassar

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Abstract

Beauveria mildew is known as useful natural control agent, due to it’s infection potential agents for controlling insect. The range of its host covers broad range of insects order including Lepidoptera, Coleoptera, Hemiptera and Hymenoptera. In the past, the behavior of B.bassiana was identified based on morphological character, which is have weakness because of environmental effect so the fenotype performance does not manifest the genotype value. Another identification method can be used to complete morphological approach is identification based on physiological behavior (toxin production) and biochemical (protein and isoenzim) where the protein analysis and isoenzim, is order to minimize the influence of environment factor for identification. The research was conducted in Laboratorium Biology UNM, there is 11 isolates B.bassiana used in this research represent location and host. Isolates were analyzed by physiological and isoenzim using electrophoresis. The result of physiological character showed that out of 11 isolates characterized, there are isolates produced beauvericin, interestingly the rest isolates produced unknown on identified metabolites. Based on total band protein pattern and isoenzim showed that there are variation, isolates from the same area or district have more similarity characters than isolates from different area.
Identification of Phytoplankton in Perintis Lake Adam, Fahria Ningsi; Chairunnisah J. Lamangantjo; Regina Valentina Aydalina; Syam S. Kumaji; Zuliyanto Zakaria; Mustamin Ibrahim
Bionature Vol. 26 No. 2 (2025): Oktober-Desember 2025
Publisher : Jurusan Biologi Universitas Negeri Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35580/9car5a55

Abstract

The purpose of this study was to determine the types of phytoplankton in Perintis Lake, Suwawa District, Bone Bolango Regency, Gorontalo Province. This research is descriptive qualitative using survey method with phytoplankton as research subject. The data collection technique was carried out by purposive sampling which was carried out 3 times with the aim of collecting data effectively. The research procedure includes observation, measurement of water physico-chemical parameters, phytoplankton water sampling, and identification in the laboratory. Data analysis was carried out descriptively by identifying the types of phytoplankton using a binocular microscope and matched to the identification book. The results of the research obtained found phytoplankton 8 divisions with 24 species (Synura sp., Pandorina morum, Ankistrodesmus densus, Tetraedron triangulare, Selenastrum bibraianum, Scenedesmus sp., Tetrabaena socialis, Dictysphaerium ehrenbergianum, Raphidiopsis raciborskii, Snowella lacustris, Merismopedia sp...), Aphanocapsa sp., Pinnularia sp., Cyclotella polymorpha, Synedra sp., Pseudostaurastrum lobulatum, Isthmochloron lobulatum, Goniochloris sp., Penium polymorphum, Staurastum gracile, Aulacoseira granulata, Cochlodinium polykrikoides, Ostreopsis sp., and Prorocentrum minimum).  
Selection and Characterization of Antagonistic Bacteria Isolated from the Rhizosphere of Potato Plants (Solanum tuberosum L.) Against the Fungus Causing Fusarium Wilt Disease In Vitro Hilda Karim; Putri Ramadhani; Nani Kurnia; Ali, Alimuddin
Bionature Vol. 27 No. 1 (2026): (In Press) April - Juni 2026
Publisher : Jurusan Biologi Universitas Negeri Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35580/jkxp9591

Abstract

Fusarium wilt, caused by Fusarium oxysporum (Fo), is a major disease affecting potato plants (Solanum tuberosum L.) and contributes significantly to yield losses. This study aimed to isolate and identify antagonistic bacteria from the potato rhizosphere capable of inhibiting the growth of F. oxysporum and to assess their antifungal potential through in vitro analysis. Soil samples were collected from the potato-growing region of Kanreapia, Gowa Regency, Indonesia. The antagonistic potential of the bacterial isolates was evaluated using the dual-culture assay. A total of eight bacterial isolates demonstrated significant inhibitory effects against Fo, representing six genera: Clostridium (75.0%), Bacillus (69.3% and 68.9%), Pseudomonas (67.9% and 62.1%), Staphylococcus (64.8%), Neisseria (64.7%), and Serratia (61.5%). Among these, the isolate identified as Clostridium exhibited the highest inhibition rate (75.0%), while Serratia showed the lowest (61.5%). These findings suggest the potential application of rhizobacteria as biological control agents for managing Fusarium wilt in potatoes.
The Effect of Variation in Light Spectra on the Growth of Chlorella Vulgaris and It is Ability to Decolorize RB5 Ummah, Faizatul; Saefi, Muhammad; Wahyudi, Didik
Bionature Vol. 26 No. 2 (2025): Oktober-Desember 2025
Publisher : Jurusan Biologi Universitas Negeri Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35580/6tkngw35

Abstract

Reactive Black 5 (RB5) is an azo dye widely used in the textile industry and improper processing of this dye can harmful to the aquatic environment, including limited light penetration and potential carcinogenic and mutagenic effects on aquatic organisms. Therefore, RB5 in waters needs to be handled seriously, one of which is through bioremediation efforts using Cholera vulgaris. Meanwhile, chlorella growth is highly dependent on various light spectrums. This study aimed to examine the effect of different light spectrums (red, blue, and white) on C. vulgaris cell density-based growth and its ability to decolorate RB5. A completely randomized design with three replications was applied using 30 mg/L RB5 solution, and was conducted for 12 days at laboratoty. Cell density was measured by a hemocytometer, and dye concentration was analyzed using a UV-Vis spectrophotometer. The results showed that blue light produced the highest algal growth, reaching 2.78 × 10⁷ cells/mL on the ninth day. Meanwhile, white light produced the greatest RB5 decolorization, reaching 80% reduction on the last day. These findings highlight the role of different light spectrums in optimizing algal cell density and dye removal efficiency.
Polysaccharide Extraction of Ulva reticulata in the Coastal Region of Nasipit, Agusan del Norte Generol, Yashica; Taperla, Princess; Badlis, Vivian; Maagdugo, Rexie
Bionature Vol. 26 No. 2 (2025): Oktober-Desember 2025
Publisher : Jurusan Biologi Universitas Negeri Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35580/yjj2hq65

Abstract

Ulvan is a sulfated polysaccharide derived from green seaweed cell walls, particularly from species within the genus Ulva. These macroalgae contain various bioactive compounds such as polysaccharides, proteins, lipids, vitamins, and dietary fiber, which contribute to their potential applications in pharmaceutical and biomedical fields. This study aimed to extract ulvan from Ulva reticulata collected from the coastal area of Nasipit, Agusan del Norte, and to characterize its functional groups using Fourier Transform Infrared (FTIR) spectroscopy. The extraction was carried out using an acid-based method, yielding approximately 3.5 g of crude polysaccharide. The structural characteristics of the polysaccharide extracts were analyzed using Fourier Transform Infrared (FTIR) spectroscopy. The FTIR spectra revealed key functional groups at specific absorption peaks: 3231-3369 cm⁻¹ (hydroxyl groups/alcohol), 1652 cm⁻¹ (amides), 1403 cm⁻¹ (aromatic rings), and 1120 cm⁻¹ (aliphatic amines). A comparative analysis was conducted between the FTIR profiles of U. reticulata obtained via acid extraction in this study and secondary data from literature involving hot water extraction of U. reticulata and acid extraction of Ulva lactuca. This comparison aims to evaluate how different extraction methods and species variations influence the presence and intensity of functional groups in the resulting ulvan.

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