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bionature
ISSN : 14114720     EISSN : 26545160     DOI : 10.35580
BIONATURE adalah jurnal yang berisi tulisan yang diangkat dari hasil penelitian, gagasan konseptual, kajian dan aplikasi teori di bidang biologi. Diterbitkan dua kali setahun pada bulan april dan oktober oleh Jurusan Biologi Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Negeri Makasar
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Articles 296 Documents
Optimalisasi Pengawetan Kurma Tomat Menjadi Serbuk Ekstrak Minuman Guna Meningkatkan Daya Simpan Pangan Nabati Ahmad Mansur; Rudi Prasetyo Ardi; Nina Mistriani
bionature Vol 23, No 2 (2022): Oktober
Publisher : Fakultas MIPA UNM

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35580/bionature.v23i2.37359

Abstract

Abstract. This research was conducted with a quantitative approach and aims to determine which tomato dates will be used as a basic ingredient in the manufacture of extract drinks. The use of this tomato analyzes the tolerance level of tomatoes to exposure to sugar derivatives (glucose and lactose) on shelf life as processed vegetable foods. Analyzing the antioxidant levels of tomato date extract based on storage time. Analyzing the effect of storage time on the organoleptic quality of tomato date extract. Analyzing the tolerance level of tomato date extract to exposure to sugar derivatives (glucose and lactose) and their impact on organoleptic quality. The results showed that the comparison of tomato date extract powder with various treatments had an effect on the parameters tested, including increasing antioxidants and reducing microbial levels and was also favored by research respondents when organoleptic testing was carried out. It can be concluded that the storage time of tomato date extract powder will increase antioxidant levels and will make the total microbial activity relatively decreased.Keywords: Dates Preservation, Beverage Extract, Shelf Life, Vegetable Food.
STUDI TUBERKULOSIS KONTAK SERUMAH PADA MASYARAKAT PESISIR DI WILAYAH KERJA PUSKESMAS SIKO KOTA TERNATE Erpi Nurdin
bionature Vol 23, No 2 (2022): Oktober
Publisher : Fakultas MIPA UNM

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35580/bionature.v23i2.37772

Abstract

Tuberkulosis paru masih menjadi penyebab kematian utama di dunia, terhitung 2 juta orang meninggal setiap tahun. WHO menyatakan bahwa sekitar 1,9 miliar orang, sepertiga penduduk dunia telah terinfeksi kuman tuberkulosis, setiap detik satu orang terinfeksi tuberkulosis di dunia. Tuberkulosis paru ditularkan melalui droplet spread dengan sumber penularan adalah pasien yang batuk dengan kontak serumah sebagai kelompok risiko tinggi penularan. Tujuan untuk mengetahui hasil pemeriksaan dan melihat persentase BTA positif atau negatif pada sampel klien yang memiliki riwayat kontak serumah dengan penderita tuberkulosis paru. Penelitian ini menggunakan metode penelitian observasional dengan pendekatan korelasi. Teknik pengambilan sampel adalah purposive sampling, yaitu pengambilan sampel berdasarkan pertimbangan peneliti. Metode pemeriksaan yang digunakan adalah metode Zielh Nelseen sebanyak 30 sampel dahak yang diperiksa. Dari 30 responden, 4 responden ditemukan positif basil tahan asam mikroskopis (BTA). 2 anggota keluarga positif, memiliki ciri-ciri perumahan padat (tidak memenuhi syarat), kelembaban dan ventilasi tidak memenuhi syarat, namun jenis lantai masih memenuhi syarat. Berdasarkan penelitian yang telah dilakukan diperoleh kesimpulan bahwa 4 (13%) responden teridentifikasi positif sedikit dengan adanya tuberkulosis di 3 tempat tuberkulosis kontak serumah, dengan faktor-faktor yang mempengaruhi terjadinya tuberkulosis di rumah tangga kontak. Tidak ada hubungan yang signifikan antara hasil pemeriksaan BTA dengan jenis kelamin (p value = 0,407 > 0,05). Tidak ada hubungan yang signifikan antara hasil pemeriksaan BTA dengan umur (p value = 0,272 > 0,05).Kata Kunci: Paru-paru, Tuberkulosis, Zielh Nelseen
Phytoremediation Ability of Ornamental Plants Celosia argantea L. and Mirabilis jalappa L. in Plumbum (Pb) Contaminated Soil Juhriah Juhriah; Dian Islamiah; Muhammad Ruslan Umar
bionature Vol 24, No 1 (2023): April
Publisher : Fakultas MIPA UNM

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35580/bionature.v24i1.43375

Abstract

Plumbum or lead is a type of heavy metal that is dangerous if it has exceeded the threshold of 0.07 µg/g based on the National Standardization Agency SNI 2004. This type of heavy metal is found in nature such as in soil, water, and air. The purpose of the study was to determine the phytoremediation ability of two types of ornamental plants, namely Celosia argentea L. and Mirabilis jalappa L., against Plumbum (Pb) metal in soil. The growth parameters observed consisted of plant height, plant stem diameter, number of leaves, leaf length, and leaf width. The ICP-MS (Inductively coupled plasma-mass spectrometry) was used to analyze the plumbum (Pb) content in soil and plants. The results ofinitial soil analysis prior to the plant planting process (phytoremediation) were 103.17 µg/g which had exceeded the predetermined threshold. The result of the analysis after phytoremediation of Celosia argentea L. showed plumbum (Pb) content of 49.685 µg/g in soil and 1,9548 µg/g in plants. Similarly, the analysis of Ph content after phytoremediation of Mirabilis jalappa L. was 47.802 µg/g in soil and 5.3077 µg/g in plants. Based on biomass calculation of Celosia argentea L. and Mirabilis jalappa L., the obtained Plumbum (Pb) content was 18.84% and 27.85%, respectively. The percentage of Pb removal of Celosia argentea L. was 51.842%, while Mirabilis jalappa L. was 53.667%. The efficiency of Pb absorption of Celosia argentea L. and Mirabilis jalappa L. was 1.895% and 5.145%, respectively. In conclusion, Ornamental plants Celosia argentea L., and Mirabilis jalappa L., are able to act as phytoremediation agents for Plumbum (Pb) metal that pollutes the soil as evidenced by the decrease in Pb levels in the soil after the phytoremediation process.Keywords: Plumbum (Pb), Phytoremediation, Celosia argentea L., Mirabilis jalappa L.
The Effect of Citronella Oil and Sesame Oil Emulsion Coating on The Quality and Storability of Mangoes (Mangifera Indica L.) Taufik Rahman; Tri Suwandi; Rena Adelia Suryani; Anisa Maharani; Chairani Azahra; Gunawan Gunawan; Lu'lu' Lathifatuzzakiyyah; N Sarah Sri Wahyuni; Nurul Annisa
bionature Vol 24, No 1 (2023): April
Publisher : Fakultas MIPA UNM

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35580/bionature.v24i1.43075

Abstract

One of the most promising agricultural commodities is the cultivation of mangoes. One way that can be used to maintain the quality and freshness of the fruit in a simple way is to do an emulsion coating. The purpose of this research was to determine the effect of citronella oil and sesame oil emulsion coating on the quality and storability of mango (Mangifera indica L.). The method used in this study was a Completely Randomized Design (CRD) with two factorial treatments and controls. The first factor was citronella oil under a concentration of 0.5% and 1%. The second factor is sesame oil under a concentration of 0.5% and 1%. The two oils were combined in the emulsion into three treatment combinations, namely S0W2, S2W0, and S1W1. The parameters measured in the observations were hardness, damage, and glucose levels in the fruit. The results showed that the level of hardness of mangoes decreased in the four treatments. Then it was found that there was an effect of delaying the maturity of the fruit with the S1W1 treatment because it was not damaged like the other treatments. While glucose levels in the fruit have increased due to the ripening process. Coating treatment with a ratio of citronella oil concentration of 0.5% to 0.5% sesame oil is the most effective treatment combination in maintaining quality and shelf life because this treatment can delay damage to mangoes. 
Characterization and Identification of Chemical Fungtional Groups in Oyster Mushroom (Pleurotus Ostreatus) Broth with edded Vegetables and Spices Siti Maulyda Ayu MZ; Halifah Pagarra; Irma Suryani Idris; Oslan Jumadi; Hartati Hartati
bionature Vol 24, No 1 (2023): April
Publisher : Fakultas MIPA UNM

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35580/bionature.v24i1.44964

Abstract

Adding the nutritional content of broth powder without destroying the distinctive taste of the broth can be done using pure mushroom extract and additional vegetables and spices. This study aims to determine the organoleptic characteristics and identify the chemical functional groups of glutamic acid in the best samples of oyster mushroom broth powder added with vegetables and spices. The results of the organoleptic test were tabulated in a table and then analyzed using a descriptive test. The organoleptic characteristics of the best color parameters were in treatment B1 which had a pale white color . The best treatment of aroma, texture, and taste parameters were found in the B2 treatment with a characteristic aroma of typical broth, fine powder texture, and has a distinctive taste of broth. The identification of the functional groups of secondary metabolites using the Fourier Transform Infrared (FTIR) instrumentation. Identification results with FTIR showed absorption at wave number (cm-1): 3419.74, 2934.62, 1639.56, 1409.70, 1055.55. There is N-H, O-H, C-H, C=O carbonyl group and carboxylic C-O, which identify the presence of amide compound, phenol acid, alkane, aldehydes, amid I and carboxylic acid, which are known as glutamic acid in this sample. Keywords: Organoleptic, Functional Groups Identification, Oyster Mushroom Broth Powder Why only B1 that you mentioned in color parameters?
Antioxidant Activities and Bioactive Compound in The Extract of Lobophytum sp. Andi Mu'nisa Syamsu
bionature Vol 24, No 1 (2023): April
Publisher : Fakultas MIPA UNM

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35580/bionature.v24i1.44786

Abstract

This study is a descriptive exploratory research that aims to know the activity of soft coral Lobophytum sp. extract as an antioxidant. Sample extraction was done by maceration using methanol. Viscous extract obtained was then partitioned using n-hexane until gained methanol and n-hexane fraction. The chemical compositions of the methanol extract of Lobophytum sp were investigated using Gas chromatography-Mass spectrometry. The yield result of soft coral Lobophytum sp. extract is 2,59%. The weight of extract partition result were methanol fraction 8,76 g and n-hexane fraction 1,02 g. Antioxidant activity test using the 2,2-Diphenyl-1-picrylhydrazyl (DPPH) assay. Ascorbic acid was also used as positive antioxidant control. The percentage of inhibition and IC50 were measured. Antioxidant activity test which gains the best antioxidant activity is methanol fraction then followed by n-hexane fraction, both showed DPPH free radical 50% can be inhibited the activity with IC50 rate in a sequence 16,93 ppm and 30,93 ppm. GC-MS analysis, 50 kinds of chemical composition are identified in the Lobophytum sp. The results showed that the soft coral extracts of Lobophytum sp. has antioxidant activity.Keywords: soft coral (Lobophytum sp), GC-MS, fractions metahol and hexane
The Effect of The Addition of Mealworm Frass (Tenebrio Molitor) and Molasses on the Increasing of the Proximate Value of White Oyster Mushroom (Pleurotus Ostreatus) Mentari Jelita Putri; Andi Faridah Arsal; Halifah Pagarra; Rachmawaty Rachmawaty; Andi Asmawati Asiz; Alimuddin Ali; Abd Muis; Muhammad Junda; Yasser Abd Djawad; Oslan Jumadi
bionature Vol 24, No 1 (2023): April
Publisher : Fakultas MIPA UNM

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35580/bionature.v24i1.44639

Abstract

This study aims to determine the effect of the addition of mealworm frass and molasses on the proximate value of white oyster mushrooms. This type of research was an experiment using a completely randomized design (CRD) consisting of four treatments with three replications each. Test parameters include water content, ash content, crude protein, crude fat and crude fiber. Proximate data on water content, ash content, crude protein, crude fat and crude fiber were analyzed using analysis of variance (ANOVA) and Tukey's follow-up test with a 95% confidence level. The results showed that treatment with frass and frass molasses had an effect on water content, ash content, crude protein, crude fat, and a decrease in fiber content. Therefore, it can be concluded that the addition of mealworm frass and molasses affects the proximate value of the resulting oyster mushrooms.Keywords: White Oyster Mushroom, Flour Caterpillar Frass, Molasses, Proximate.
Vegetative Growth Response of Corn (Zea mays) Against Actinomycetes, Azospirillum, and Azotobacter from the Rhizosphere Neem (Azadirachta indica) Yusminah Hala; A.Irma Suryani; Octavia Cisiliana Devi
bionature Vol 24, No 1 (2023): April
Publisher : Fakultas MIPA UNM

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35580/bionature.v24i1.44115

Abstract

This research is an experimental study that aims to determine the effect of the application of N2-fixing bacteria Actinomycetes, Azospirillum, and Azotobacter on the vegetative growth parameters of corn plants and on the nitrogen content of corn plants. Parameters for measuring the growth of corn plants include: formation rate of radicle length, plant height, number of leaves, leaf length, fresh weight of plants, fresh weight of plant roots, dry weight of plants, dry weight of plant roots, total N content in plants, as well as physical and chemical qualities of the ground. Experiments on the application of N2 -fixing bacteria on corn plants on a laboratory scale were carried out to determine the in vitro effect of microbes on corn seeds. The pot test was carried out to determine the effect of N2-fixing bacteria on the vegetative growth of corn plants in vivo. Data were analyzed using ANOVA with Duncan's test. The results showed that corn plants inoculated with Actinomycetes, Azospirillum and Azotobacter showed a significant different growth from corn plants without nitrogen fixing bacteria, namely formation rate of radicle length, plant height, number of leaves, leaf length, fresh weight, dry weight, total N content (%) of crops, and soil physical and chemical quality. It can be concluded that the inoculation of nitrogen-fixing bacteria in corn plants has a significant effect on plant vegetative growth parameters and plant nitrogen content.Keywords: Nitrogen fixing bacteria, Actinomycetes, Azospirillum, Azotobacter, Plant vegetative growth.
Identification and Description of Aquatic Oligochaeta in Sanggau City Canal West Kalimantan Galuh Ramadhanti
bionature Vol 24, No 1 (2023): April
Publisher : Fakultas MIPA UNM

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35580/bionature.v24i1.43532

Abstract

The canals in the center of Sanggau City, West Kalimantan serve as a medium for wastewater which enters the Kapuas River. The density of community activities in this city has caused indications of its canals being polluted by organic and inorganic materials. These contaminants enter the canal continuously, hence its accumulation can change the texture of the substrate/sediment which is the habitat for aquatic Oligochaete. The purpose of this study was to identify and describe the types of aquatic Oligochaete in the canals of Sanggau City. The research was conducted from August to September 2020 using the purposive sampling method at five stations, namely Tanjung Sekayam Village, Ilir Kota Village, Beringin Village, and Bunut Village, with three replications at each sampling station. The results showed that aquatic Oligochaete at the canals of Sanggau City consisted of 1 order, 1 family, 2 genera, and 3 species with a total of 1367 individuals. The identified species are Branchiura sowerbyi, Limnodrilus claparedeianus, and Limnodrilus hoffmeisteri.Keywords: Species Identification, Aquatic Oligochaeta, Sanggau City Canal
Some Properties of Oyster Mushroom Grown on Substrates Containing Paper Waste Joko Sulistyo
bionature Vol 24, No 2 (2023): Oktober
Publisher : Fakultas MIPA UNM

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35580/bionature.v24i2.45548

Abstract

ABSTRACTThis study was conducted to determine the activity of starter culture of oyster mushroom and influence of substrates containing paper waste materials used to grow the mushroom. The purpose of the study was to analyze nutritional value and antioxidant activity of the mushroom grown on the substrate contained paper waste. The substrate was prepared using paper waste with 5 different formulations were 0% (control), 20%, 30%, 40%and 50% paper waste. Proximate analysis, heavy metal content and antioxidant activity were carried out to test the quality of the mushroom. Overall, the mushroom grown on the substrates containing paper waste showed high water content, however, the mushroom grown on the substrate contained 50% paper waste showed the highest content of ash, protein, and crude fiber, while higher fat content showed by the mushroom grown on the substrate contained 50% paper waste compared to the mushroom grown on other substrate with different composition of paper waste. The mushroom grown on the substrate contained 50% paper waste indicated considerably high content of heavy metals such as lead, (3.5%), copper (20.8%) and cadmium (4.4%), respectively however, it showed the higher antioxidant activity (87.19%) as well compared to the mushrooms grown on the substrate containing a lower percentage of paper waste, thus giving a conclusion that there was probably positive correlation in between the heavy metals contained in paper waste with antioxidant activity. Keywords: paper waste, oyster mushroom, proximate analysis, antioxidant, heavy metals. ABSTRAKPenelitian ini dilakukan untuk menentukan aktivitas kultur starter jamur tiram dan menguji substrat yang mengandung limbah kertas yang digunakan untuk menumbuhkan jamur. Tujuan penelitian adalah untuk menganalisis nilai gizi dan aktivitas antioksidan dari jamur yang ditumbuhkan pada substrat mengandung limbah kertas. Substrat disiapkan menggunakan limbah kertas dengan 5 formula berbeda yaitu 0% (control), 20%, 30%, 40% dan 50%. Analisis proksimat, kandungan logam berat dan aktivitas antioksidan dilakukan untuk menguji kualitas dari jamur. Secara keseluruhan, jamur tiram yang ditumbuhkan pada substrat limbah kertas menunjukkan kadar air yang tinggi, namun jamur yang ditumbuhkan pada substrat mengandung 50% limbah kertas menunjukkan kadar abu, protein, dan serat kasar yang tertinggi, sementara kadar lemak pada jamur yang ditumbuhkan pada substrat mengandung 50% limbah kertas menunjukkan lebih tinggi dibandingkan dengan jamur yang ditumbuhkan pada substrat dengan komposisi limbah kertas lainnya. Jamur yang ditumbuhkan pada substrat mengandung 50% limbah kertas menunjukkan kandungan logam berat, masing-masing seperti timbal (3.5%), tembaga (20.8%) dan kadmium (4.4%) yang cukup tinggi, namun juga menunjukkan aktivitas antioksidan yang lebih tinggi (87.19%) dibandingkan dengan jamur yang ditumbuhkan pada substrat mengandung persentase limbah kertas yang lebih rendah, sehingga memberikan kesimpulan bahwa terdapat korelasi yang positif antara logam berat yang terkandung dalam limbah kertas dengan aktivitas antioksidan.Kata kunci: limbah kertas, jamur tiram, nilai gizi, analisis proksimat. Antioksidan, logam berat.