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Journal of Aquaculture Management and Technology
Published by Universitas Diponegoro
ISSN : -     EISSN : -     DOI : -
Core Subject : Science, Education,
Computer Science & IT Decision Sciences, Operations Research & Management Other
Journal of Aquaculture Management and Technology diterbitkan oleh Program studi Budidaya Perairan, Fakultas Perikanan dan Ilmu Kelautan, Undip. JAMTech menerima artikel-artikel yang berhubungan dengan akuakultur, nutrisi pakan ikan, parasit dan penyakit ikan, produksi budidaya, dll.
Arjuna Subject : -
Articles 305 Documents
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PEMBERIAN EKSTRAK KASAR DAUN TEMBAKAU (Nicotiana tabacum) UNTUK MENGENDALIKAN INFESTASI ARGULUS sp. PADA IKAN KOMET (Carassius auratus auratus) Gultom, Dian Sary; Desrina, - -; Sarjito, - -
Journal of Aquaculture Management and Technology Volume 7, Nomor 1, Tahun 2018
Publisher : Journal of Aquaculture Management and Technology

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Abstract

Penyakit Argulosis adalah salah satu penyakit yang menyerang ikan komet yang disebabkan oleh parasit Argulus sp. Salah satu upaya pengendalian serangan Argulus sp. yaitu menggunakan insektisida alami. Ekstrak kasar daun tembakau (N. tabacum) merupakan salah satu bahan alami yang dapat digunakan sebagai alternatif pengobatan Argulus sp. Penelitian ini bertujuan untuk mengetahui pengaruh perendaman ekstrak daun tembakau terhadap kelulushidupan ikan komet dan mortalitas Argulus sp. Metode penelitian yang digunakan adalah eksperimental dengan 4 perlakuan dan 3 kali ulangan. Dosis yang digunakan pada penelitian ini yaitu perlakuan A (0 ml/L), perlakuan B (3 ml/L), perlakuan C (6 ml/L) dan perlakuan D (9 ml/L). Ikan uji yang digunakan adalah ikan komet sebanyak 60 ekor dengan panjang tubuh rata-rata 6,42±0,45 cm dan bobot tubuh rata-rata 7,61±0,72 gram, kemudian Argulus sp.sebanyak 120 ekor. Uji in vivo dilakukan dengan cara menginfeksikan Argulus sp. dengan kepadatan 30 ekor Argulus sp. ke dalam tiap-tiap perlakuan selama 12 hari, setelah muncul gejala klinis kemudian dilakukan perendaman menggunakan ekstrak kasar daun tembakau selama 60 menit. Pengamatan dilakukan selama 7 hari dengan parameter pengamatan meliputi gejala klinis ikan, kelulushidupan ikan, mortalitas Argulus sp. dan kualitas air. Hasil penelitian menunjukan bahwa pemberian ekstrak kasar daun tembakau tidak berpengaruh nyata (P>0,05) terhadap kelulushidupan ikan komet yang diinfeksi Argulus sp. Nilai kelulushidupan tertinggi terdapat pada perlakuan B sebesar 80,00% diikuti oleh perlakuan C sebesar 73,33%, perlakuan D sebesar 60,00% dan pada perlakuan A sebesar 46,67%. Penggunaan ekstrak daun tembakau terbukti berpengaruh terhadap mortalitas Argulus sp. yaitu 25 ekor (Perlakuan D), 19 ekor (perlakuan C), 12 ekor (perlakuan B), dan 5 ekor (perlakuan A). Hasil penelitian diperoleh bahwa pemberian ekstrak daun tembakau pada perlakuan D (9 ml/L) merupakan dosis yang dapat menyebabkan mortalitas yang tinggi pada Argulus sp. dengan tingkat mortalitas 83,33%.Argulosis is a common disease infectcomet fish caused by Argulus sp.parasites. One of the efforts to control Argulus sp. attack that was used natural insecticide. Tobacco leaf extract (N. tabacum) was one of the natural ingredients that can be used as an alternative treatment of Argulus sp. This study aims to determine of immersed the effect of tobacco leaf extract on survival rate and the effect of immersed of tobacco leaf extract (N. tabacum) on mortality of Argulus sp. The experiment consisted of 4 treatments each with 3 replications. Doses used in this study were treatment A (0 ml/L), treatment B (3 ml/L), treatment C (6 ml/L) and treatment D (9 ml/L). 60 comet were used in this study with an average body length of 6,42±0,45 cm and an average body weight of 7,61±0,72 grams, and then infested by 120 Argulus sp.The experimental comet were infected with Argulus sp. at density 30 Argulus sp.were conducted for 12 days, after injection showed clinical signs comet were immersed in tobacco leaf extract about 60 menit. The observations were conducted for 7 days with observation parameters including clinical symptoms, survival rate, mortality Argulus sp. and water quality. The result showed that immersed tobacco leaf extract had no significant (P>0.05) of survival rate. of comet fish infected by Argulus sp.The highest survival rate in treatment B (80,00%) treatment C (73,33%) treatment D (60,00%) and treatment A46,67%. The result showed that immersed tobacco leaf extracts had significant effect of mortality Argulus sp. is 25 Argulus sp. (Treatment D), 19 Argulus sp. (treatment C), 12 Argulus sp. (treatment B), and 5 Argulus sp. (treatment A). The results showed that immersion in treatment D (9 ml/l) was a dose that could cause high mortality in Argulus with 83,33%.   Argulosis is a common disease infectcomet fish caused by Argulus sp.parasites. One of the efforts to control Argulus sp. attack that was used natural insecticide. Tobacco leaf extract (N. tabacum) was one of the natural ingredients that can be used as an alternative treatment of Argulus sp. This study aims to determine of immersed the effect of tobacco leaf extract on survival rate and the effect of immersed of tobacco leaf extract (N. tabacum) on mortality of Argulus sp. The experiment consisted of 4 treatments each with 3 replications. Doses used in this study were treatment A (0 ml/L), treatment B (3 ml/L), treatment C (6 ml/L) and treatment D (9 ml/L). 60 comet were used in this study with an average body length of 6,42±0,45 cm and an average body weight of 7,61±0,72 grams, and then infested by 120 Argulus sp.The experimental comet were infected with Argulus sp. at density 30 Argulus sp.were conducted for 12 days, after injection showed clinical signs comet were immersed in tobacco leaf extract about 60 menit. The observations were conducted for 7 days with observation parameters including clinical symptoms, survival rate, mortality Argulus sp. and water quality. The result showed that immersed tobacco leaf extract had no significant (P>0.05) of survival rate. of comet fish infected by Argulus sp.The highest survival rate in treatment B (80,00%) treatment C (73,33%) treatment D (60,00%) and treatment A46,67%. The result showed that immersed tobacco leaf extracts had significant effect of mortality Argulus sp. is 25 Argulus sp. (Treatment D), 19 Argulus sp. (treatment C), 12 Argulus sp. (treatment B), and 5 Argulus sp. (treatment A). The results showed that immersion in treatment D (9 ml/l) was a dose that could cause high mortality in Argulus with 83,33%.
STUDI KESESUAIAN LAHAN BUDIDAYA IKAN KERAPU DALAM KARAMBA JARING APUNG DENGAN APLIKASI SISTEM INFORMASI GEOGRAFIS DI TELUK RAYA PULAU SINGKEP, KEPULAUAN RIAU Hasnawiya, Hasna
Journal of Aquaculture Management and Technology Vol 1, No 1 (2012): Journal Of Aquaculture Management and Technology
Publisher : Journal of Aquaculture Management and Technology

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Abstract

Ketepatan pemilihan lokasi adalah salah satu faktor yang menentukan keberhasilan usaha budidaya ikan kerapu. Melalui perkembangan teknologi secara umum dewasa ini, Sistem Informasi Geografis (SIG) merupakan salah satu pilihan dalam penentuan lokasi untuk pengembangan budidaya laut, khususnya ikan kerapu. Tujuan penelitiaan adalah menentukan kesesuaian lokasi perairan yang berpotensi untuk melakukan kegiatan budidaya ikan kerapu dalam karamba jaring apung di Teluk Raya, Pulau Singkep Kepulauan Riau berdasarkan model spasial variabel data lapangan. Penelitian ini diawali dengan survey lapangan pada bulan Maret 2012 di Teluk Raya, Pulau Singkep Kepulauan Riau, kemudian pengumpulan data dan pengambilan sampel pada lokasi tersebut bulan April 2012 selanjutnya analisis data di Balai Penelitian dan Observasi Laut, Jembrana Bali dan di Fakultas Perikanan dan Ilmu Kelautan, Universitas Diponegoro Semarang. Metode yang dipakai dalam penelitian ini adalah metode survey. Penentuan titik lokasi sampling dengan metode purposive sampling yaitu dengan mengacu pada fisiografi lokasi. Metode penelitian ini meliputi dua tahapan yaitu pengumpulan data yang terdiri dari 7 parameter kualitas air (DO, pH, suhu, salinitas, arus, kecerhana dan kedalaman) dan analisa data. Hasil pengukuran kecepatan arus berkisar antara 0.05 – 0,31 m/s, suhu 30oC - 32oC, salinitas 30,1 - 33,0 ppt, DO 4,8 mg/l-5,8 mg/l, kecerahan 1,72 m – 4,38 m dan pH 8.1-8.2. Hasil model spasial kelas kesesuaian lahan menunjukkan bahwa perairan Teluk Raya dengan luas ±84,30 km2 cukup potensial untuk dilakukan usaha budidaya ikan kerapu dengan sistem keramba jaring apung yang terbagi kedalam 3 kelas kesesuaian yaitu : sangat sesuai (3,9 - 5), sesuai (2,7 - 3,8) dan tidak sesuai (1 - 2,6).    
PENGARUH PEMBERIAN REKOMBINAN HORMON PERTUMBUHAN (rGH) MELALUI METODE PERENDAMAN DENGAN LAMA WAKTU YANG BERBEDA TERHADAP PERTUMBUHAN DAN KELULUSHIDUPAN IKAN LELE VARIETAS SANGKURIANG Triwinarso, Wisnu Hadi; Basuki, Fajar; Yuniarti, Tristiana
Journal of Aquaculture Management and Technology Volume 3, Nomor 4, Tahun 2014
Publisher : Journal of Aquaculture Management and Technology

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Abstract

Penelitian ini bertujuan untuk mengkaji pengaruh pertumbuhan benih ikan lele sangkuriang yang diberi rekombinan hormon pertumbuhan dosis 2mg/L dengan metode perendaman dengan lama waktu yang berbeda dan mengkaji waktu yang optimal benih ikan lele sangkuriang yang diberi rekombinan hormon pertumbuhan terhadap pertumbuhan dan kelangsungan hidup benih ikan lele sangkuriang. Penelitian ini dilaksanakan di Satker PBIAT Ngrajek, Magelang, pada bulan Agustus-November 2013. Penelitian menggunakan rancangan acak lengkap (RAL) dengan 4 perlakuan dan 3 ulangan. Perlakuan perendaman dengan larutan rekombinan hormon pertumbuhan 2mg/L perlakuan A selama 0 menit, perlakuan B 30 menit, perlakuan C 60 menit, dan perlakuan D 90 menit. rGH yang digunakan berasal dari ikan kerapu kertang (ElrGH). Hasil pengamatan pertumbuhan bobot spesifik perlakuan A sebesar 5,642±0,025 %/hari, perlakuan B sebesar 6,510±0,055 %/hari, perlakuan C sebesar 6,358±0,108 %/hari, perlakuan D sebesar 6,240±0,179 %/hari. Pengukuran panjang mutlak didapat hasil perlakuan A mendapatkan hasil 4,18±0,07 cm, pelakuan B 5,35 ± 0,03 cm, perlakuan C 5,30 ± 0,09 cm, dan perlakuan D 5,25 ± 0,23 cm. Nilai konversi pakan pada perlakuan A 0,751±0,008, perlakuan B 0,457±0,022, perlakuan C 0,514±0,010, perlakuan D 0,543±0,008. SR yang didapat selama pemeliharaan pada perlakuan A 82,67±1,53%, Perlakuan B 79,00±3,00% , perlakuan C 75,33±2,52%, perlakuan D 73,00±1,00%. Pemberian rekombinan hormon pertumbuhan melalui metode perendaman pada ikan lele sangkuriang dengan lama waktu 30 menit dapat meningkatkan pertumbuhan bobot spesifik, pertumbuhan panjang mutlak, dan kelulushidupan, serta menurunkan rasio konversi pakan. Pemberian rekombinan hormon pertumbuhan dapat meningkatkan SGR sebesar 15,90%, panjang mutlak 28%, kelulushidupan 13,25%, dan menurunkan FCR 64,33% The purpose of this study was to assess the effect of seed growth sangkuriang catfish fed with recombinant growth hormone dose 2mg / L through immersion method with different time and assess the optimal time sangkuriang seed catfish fed recombinant growth hormone on growth and seed survival rate catfish sangkuriang. This study was conducted in Unit Freshwater Fish Hatchery Center (SATKER PBIAT) Ngrajek, Magelang, August-November 2013. Research using a completely randomized design (CRD) with 4 treatments and 3 replications. Immersion treatment with recombinant growth hormone solution of 2 mg / L treatment A for 0 min, 30 min treatment B, treatment of C 60 mins, and treatment D 90 minutes. The rGH used from giant grouper fish (rElGH). The observation of SGR treatment of A 5,642±0,025 %/day, treatment of B 6,510±0,055 %/day, treatment of C 6,358±0,108 %/day, treatment of D 6,240±0,179 %/day. The absolute length measurements that showed growth optimum results at A 4,18±0,07 cm, treatment of B 5,35 ± 0,03 cm, treatment of C 5,30 ± 0,09 cm, and treatment of D 5,25 ± 0,23 cm. Feed conversion value in treatment A 0,751±0,008, treatment B 0,457±0,022, treatment C 0,514±0,010, treatment D 0,543±0,008. SR obtained during the maintenance treatment A 82,67±1,53%, treatment B 79,00±3,00% , treatment C 75,33±2,52%, treatment D 73,00±1,00%. Administration of recombinant growth hormone through immersion method at catfish sangkuriang long 30 minutes can increase the growth of a specific weight, length of absolute growth, and survival, as well as lower feed conversion ratio. Administration of recombinant growth hormone can increase by 15.90% SGR, the absolute length of 28%, the survival of 13.25%, and 64.33% lower FCR
PENAMBAHAN TEPUNG BUNGA MARIGOLD ( Tagetes Erecta) PADA PAKAN BUATAN UNTUK MENINGKATKAN KECERAHAN WARNA IKAN RAINBOW (Melanotaenia pearcox) Merlin, Nadia Punky Uberthi; Samidjan, Istiyanto; Pinandoyo, - -
Journal of Aquaculture Management and Technology Volume 6, Nomor 3, Tahun 2017
Publisher : Journal of Aquaculture Management and Technology

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Abstract

Ikan  rainbow (Melanotaenia pearcox)merupakan salah satu ikan hias endemik asal Papua. Warna, bentuk tubuh, serta gerak geriknya menjadi daya tarik masyarakat umum untuk memeliharanya, Ikan  rainbow (Melanotaenia pearcox) memiliki  nilai  ekonomis  tinggi  sebagai  ikan  hias  karena  penampilan  warna  dan  ukuran yang  menarik. Warna pada tubuh ikan hias dapat dihasilkan dari pakan yang mengandung zat warna. Analisis warna yang digunakan adalah Adobe Photoshop CC. Penelitian ini bertujuan unruk mengetahui pengaruh penambahan tepung bunga marigold terhadap performa warna tubuh dengan dosis yang sesuai. Dosis yang digunakan adalah perlakuan A tanpa penambahan tepung bunga marigold, perlakuan B dengan dosis 1,5%, perlakuan C 2%,  dan perlakuan D 2,5% Analisa data menggunakan ANNOVA dan apabila terdapat berpengaruh sangat nyata (P<0,01) maka dilanjutkan dengan uji Duncan. Hasil penelitian menunjukkan bahwa penambahan tepung bunga marigold dalam pakan buatan pada perlakua A yaitu sebesar 24,92±4,87, perlakuan B yaitu sebesar 42,58±2,46, , perlakuan C yaitu sebesar 58,05±1,34, dan perlakuan D yaitu sebesar 36,80±3,88. Kualitas air selama penelitian masih berada dalam kisaran yang layak untuk kehidupan ikan rainbow yaitu suhu 25 - 28ºC; pH 7,2 – 7,9; DO 4,7 - 4,9mg/L; amoniak 0,61 - 0,94mg/L. Kesimpulan dari penelitian ini adalah adanya pengaruh sangat nyata terhadap penambahan tepung bunga marigold dalam pakan buatan terhadap peningkatan performa warna tubuh ikan rainbow dengan dosis terbaik 2% . Rainbow fish (Melanotaenia pearcox) is one of the original ornamental fish endemic to New Guinea. Color, body shape, as well as the motion of the appeal of the people to keep it, the Rainbow Fish (Melanotaeniidae pearcox)has a high economical value as ornamental fish due to the appearance of color and size. The analysis of colors used is Adobe Photosop CC. The colors of the fish can be  produced from the feed that carotenoid. The purpose of the research to determine the influence of the addition of  marigold flower  flour to fish body  color performance   with the appropriate dose. The doses used on treatment A without the addition of marigold flower flour, treatment B 1.5%,treatment C 2%  and treatment D 2.5%. Data analysis used ANNOVA and when highly significant effect (P<0,01)  then continued with by Duncan test. The results showed that the addition marigold flower flour in artificial feed on treatmen A 24,92 ± 4, 87, treatment B 42,58 ± 2, 46,  treatment C 58,05 ± 1.34  and  treatment D  36,80 ± 3, 88. The water quality during the research was still in a decent range of rainbow fish. That temperature was  25-28 ° C; pH 7.2 – 7.9; DO 4.7-4, 9mg/L; ammonia 0.61-0, 94mg/L. The conclusion of this research was the significant influence of the addition of marigold flower flour in artificial feed to increased performance of body color rainbow fish with the best dose of 2% .
Identifikasi Dan Uji Postulat Koch Agensia Penyebab Penyakit Bakteri Pada Ikan Lele (Clarias gariepinus) yang Berasal dari Demak Wijayanti, Aulia Resty; -, Sarjito; Prayitno, Slamet Budi
Journal of Aquaculture Management and Technology Vol 2, No 2 (2013) : Journal of Aquaculture Management and Technology
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Abstract

The aim of the research was to find out the clinical sign of the catfish sample that has been infected by the bacteria and to find out the causative agent bacteria diseases of Clarias gariepinus from Demak. The fish sample collection used purposive random sampling method. The observation of clinical sign was implemented toward ten catfish samples. The isolation of bacteria from moribund fish surface and kidney of catfish was conducted by streaking method on NA and GSP media. Based on the characteristic of morphology (colour, form of colony), it was obtained five isolate from eleven isolates of bacteria for the next test. The test of postulat koch toward five isolates of ten test fish and characterized them to causative agent bacterial through morphological and biochemical characterization. The result of the research showed that the clinical sign were detected irregular swimming, balance disorders, fish stand still at the bottom of the aquarium, the fish limp, injury/red blisters at the injection site, a pale body color, porous on tails, fins and antennas. The test results of postulates koch obtained that the five isolates (D7, D10, D12, D14, D16) were capable of causing pain on test fish. Based on the postulate test also showed only two isolates (D7 and D12) were caused mortality of 30% of the test fish. The results of the morphological and biochemical characterization found that the five agents causing disease in fish from Demak were abbreviated namely of Pseudomonas plecoglossicida, Flavobacterium sp., Edwardsiella ictaluri, Aeromonas caviae and Aeromonas caviae, respectively.
STUDI KASUS INFESTASI Cichlidogyrus PADA IKAN NILA (Oreochromis niloticus) DARI SATKER BPBIAT DAN LUAR SATKER BPBIAT JANTI, KLATEN, JAWA TENGAH Kamil, Mia Rahmah; Prayitno, Slamet Budi; Desrina, - -
Journal of Aquaculture Management and Technology Volume 6, Nomor 4, Tahun 2017
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Abstract

Cichlidogyrus merupakan salah satu parasit dari golongan monogenea yang meninfestasi ikan nila. Cichlidogyrus menyerang pada insang yang menempel pada filamen insang dan memakan sel-sel epitel insang, mukus dan darah pada insang. Hal ini dapat menimbulkan kematian bagi ikan-ikan nila yang masih muda atau dalam keadaan lemah akibat stres dan infeksi akut. Penelitian ini bertujuan untuk mengetahui gejala klinis ikan yang terinfestasi Cichlidogyrus, morfologi dari Cichlidogyrus, jumlah, prevalensi, intensitas Cichlidogyrus serta uji statistika perbandingan antara jumlah Cichlidogyrus, prevalensi dan intensitas antara sampel Satker BPBIAT Janti dan Masyarakat Desa Janti. Metode yang digunakan yakni monitoring menggunakan data primer dan data sekunder. Materi yang digunakan yaitu ikan nila sebanyak 252 ekor di ambil secara acak dari kolam Satker BPBIAT dan kolam Masyarakat Desa Janti. Pengamatan ektoparasit dilakukan dengan pembuatan preparat segar dan selanjutnya diamati dibawah mikroskop. Hasil dari penelitian ini yakni gejala klinis yang tampak pada ikan nila yakni berupa produksi lendir berlebih pada insang dan warna insang merah pucat. Cichlidogyrus mempunyai tubuh pipih memanjang, memiliki sepasang mata, faring yang berbentuk bulat, cuping kepala atau cephalic lobe, organ reproduksi, haptor yang terdiri dari 2 pasang jangkar dan 7 pasang kait. Jumlah Cichlidogyrus pada kolam Satker BPBIAT Janti 1169 individu dan 641 individu pada Kolam Masyarakat Desa Janti. Prevalensi Cichlidogyrus pada Satker BPBIAT Janti 65,87% dan kolam Masyarakat Desa Janti 69,04%. Intensitas rata-rata Cichlidogyrus sebesar 12 ind/ekor pada kolam Satker BPBIAT Janti  dan 6 ind/ekor pada kolam Masyarakat Desa Janti. Uji t independen perbandingan jumlah Cichlidogyrus, prevalensi dan intensitas tidak berbeda nyata antara sampel Satker BPBIAT Janti dan Masyarakat Desa Janti. Dapat disimpulkan bahwa dua lokasi tidak berbeda signifikan terhadap jumlah Cichlidogyrus, prevalensi dan intensitas. Cichlidogyrus is one of the monogenean parasites that infestated tilapia. Cichlidogyrus anchored the gill filaments and fed on the epithelial cells, mucus and blood of the gills. This could lead to death for young tilapia or weaken due to stress and acute infection. The aim of this research were to observe the clinical symptoms of Cichlidogyrus infected fish, Cichlidogyrus morphology, infestation, prevalence, and intensity between Satker BPBIAT Janti and Janti Village Community were compared. Monitoring of the both pond sites and interviewed were carried out to abtain primary and secondary data. The material used were 252 tilapia fish taken random by from Satker BPBIAT ponds and Janti Village Community Ponds. Ectoparasite observations were performed by fresh smears  and subsequently observed under a microscope. The results showed that  clinical symptoms appeared in the tilapia were excess of mucus production in the gills and pale gill color. Cichlidogyrus has elongated flat body, a pair of eyes, a round pharynx, a head lobe or a cephalic lobe, a reproductive organ, a haptor consisting of 2 pairs of anchors and 7 pairs of hooks. Number of Cichlidogyrus in Satker BPBIAT Janti were 1169 individuals while in Janti Village Community Ponds were 641 individuals. Prevalence of Cichlidogyrus on Satker BPBIAT Janti was 65,87% and Janti Village Community Pool was 69,04%. Intensity of Cichlidogyrus amounted to 12 ind / tail and 6 ind / tail on Satker BPBIAT Janti and Janti Village Community Ponds respectively. The independent t-test of Cichlidogyrus comparison, in prevalence and intensity have no significant differences. It can be concluded that those two location has no significant difference on Cichlidogyrus infestation, prevalence and intensity.
Gambaran Parameter Hematologis Pada Ikan Nila (Oreochromis niloticus) Yang Diberi Vaksin DNA Streptococcus iniae Dengan Dosis Yang Berbeda Utami, Devitha Tri; Prayitno, Slamet Budi; Hastuti, Sri; Santika, Ayi
Journal of Aquaculture Management and Technology Vol 2, No 4 (2013) : Journal of Aquaculture Management and Technology
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Abstract

Tilapia (O. niloticus) is one of freshwater species that has rapid growth and easily been cultivated. One of the constraints in cultivating Tilapia is Streptococciasis disease caused by Streptococcus iniae. The purpose of this research was to determine the haematological response of Tilapia that was given by DNA vaccine of S. iniae and challenged with S. iniae, and to determine the most effective dose of DNA vaccine based on haematological performances in the prevention of bacteria S. iniae. The fish samples used were Tilapia with length of 7,79 cm ± 0.48 as many as 150 animals or 10 animals per treatment. Five (5) treatments and three (3) replications were administered in this research, there were A (0 ŋg/µL), B (10ŋg/µL), C (20ŋg/µL), D (30ŋg/µL), dan E (40ŋg/µL) of DNA vaccines the injected intramuscularly. Fish were maintained for 30 days, then challenged with S. iniae for 14 days. Measurement of haematological performances include haematocrit, total leukocyte, differential leukocytes, and phagocytosis index carried out every 7 days. The observation of clinical signs of fish after challenge by S. iniae obtained that fish in A and B treatment showed clinical signs more quickly than fish in C, D, and E treatment. The results showed that DNA vaccines S. iniae in E treatment significantly different on haematocrit levels (25,67%), total leukocytes (9,46x104 cells/mm3), lymphocytes (80,33%), monocytes (5,67%), neutrophils (14%), phagocytosis index (22,33%), and survival rate (70%). It can be conclude that dosage of DNA vaccine 40ng/μl is the most effective dosage which is immunogenic and protective in raising Tilapia immunity against bacterial S. iniae based on haematological aspects.
PERFORMA PRODUKSI IKAN LELE DUMBO (Clarias gariepinus) YANG DIPELIHARA DENGAN SISTEM BIOFILTER AKUAPONIK DAN KONVENSIONAL Wicaksana, Satria Nawa; Hastuti, Sri; Arini, Endang
Journal of Aquaculture Management and Technology Volume 4, Nomor 4, Tahun 2015
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Abstract

Kenaikan produksi budidaya di Indonesia diupayakan dengan memanfaatkan lahan secara optimal. Budidaya sistem tertutup akan memproduksi limbah dari hasil metabolisme yang secara berkelanjutan mencapai level yang beracun bagi ikan. Salah satu upaya untuk menanggulangi masalah tersebut yaitu pengelolaan lingkungan budidaya dengan sistem biofilter akuaponik. Tujuan dari penelitian ini adalah untuk mengetahui performa produksi seperti pertumbuhan, kelulushidupan ikan lele dumbo (Clarias gariepinus), pertumbuhan kangkung dan mengetahui efektivitas sistem biofilter akuaponik terhadap penurunan amonia. Penelitian dilakukan pada bulan Desember 2014–Maret 2015 di Laboratorium Budidaya Perairan, Universitas Diponegoro, Semarang. Variabel yang dikaji meliputi laju pertumbuhan spesifik (SGR) (%bobot/hari), biomassa mutlak (g), kelulushidupan (SR) (%), tingkat konsumsi pakan (g), rasio konversi pakan (FCR), efisiensi pemanfaatan pakan (%), efektivitas sistem biofilter akuaponik, rata-rata bobot kangkung (g) dan kualitas air. Penelitian menggunakan rancangan acak lengkap (RAL) dengan 2 perlakuan dan 3 ulangan, yaitu perlakuan A (sistem biofilter akuaponik) dan perlakuan B (sistem konvensional). Hasil penelitian menunjukkan bahwa sistem biofilter akuaponik dan sistem tanpa biofilter akuaponik (konvensional) berbeda sangat nyata (sign<0,01) terhadap SGR, biomassa mutlak (g), FCR dan efisiensi pemanfaatan pakan dan berbeda nyata (sign<0,05) terhadap SR dan tingkat konsumsi pakan. Sistem biofilter akuaponik memberikan hasil lebih baik dibandingkan dengan sistem konvensional. Hasil dari sistem biofilter akuaponik yaitu SGR 4,21±0,06 %bobot/hari, biomassa mutlak 29353,0±2417,58 g, SR 92,71±1,88 %, tingkat konsumsi pakan 36166,67±1527,52 g, FCR 1,15±0,02, efisiensi pemanfaatan pakan 81,07±3,30%. Kadar amonia dalam sistem ini masih dalam kisaran yang layak untuk pembesaran ikan lele dumbo hingga minggu ke 8. Berdasarkan hasil penelitian ini maka dapat disimpulkan bahwa sistem biofilter akuaponik dapat meningkatkan performa produksi ikan lele. The increase in aquaculture production in Indonesia pursued by utilizing land optimally. Closed system culture will produce waste from the metabolism which sustainably reach the level that are toxic to fish. One effort to overcome the problem that the environmental management of aquaculture with biofilter aquaponics system. This study was aimed to know the production performance such as growth, survival of african catfish (Clarias gariepinus), spinach water growth and to know the effectiveness of the biofilter aquaponics system to decrease ammonia. The study was conducted on December 2014-March 2015 in the Laboratory of Aquaculture, Diponegoro University, Semarang.Variables that were examined include the specific growth rate (SGR) (% weight / day), an absolute biomass (g), survival rate (SR) (%), the level of feed intake (g), feed conversion ratio (FCR), efficiency feed utilization (%), the effectiveness of the biofilter aquaponics system, the average weight of spinach water (g) and water quality. Research using a completely randomized design (CRD) with 2 treatments and 3 replications, namely treatment A (biofilter aquaponics system) and treatment B (conventional system).The results showed that the biofilter aquaponics system and the system without a biofilter aquaponics (conventional) highly significant different (sign <0.01) on SGR, an absolute biomass (g), FCR and feed utilization efficiency and significantly different (sign <0.05) on SR and the feed consumption rate. Biofilter aquaponics system gives better results than conventional systems. Results of aquaponics biofilter system that was SGR 4.21 ± 0.06% weight / day, an absolute biomass 29353.0 ± 2417.58 g, SR 92.71 ± 1.88%, the level of feed intake 1527.52 ± 36166.67 g, FCR 1.15 ± 0.02, 81.07 ± 3.30%  efficiency of feed utilization. Levels of ammonia in the system was still in a reasonable range for fish rearing african catfish until week 8. Based on these results it can be concluded that the biofilter aquaponics system can improve production performance of catfish.
INFEKSI WHITE SPOT SYNDROM VIRUS (WSSV) PADA UDANG WINDU (Penaeus monodon Fabr.) YANG DIPELIHARA PADA SALINITAS MEDIA YANG BERBEDA Rahma, Hardyta Noviar; Prayitno, Slamet Budi; Haditomo, Alfabetian Harjuno Condro
Journal of Aquaculture Management and Technology Volume 3, Nomor 3, Tahun 2014
Publisher : Journal of Aquaculture Management and Technology

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Abstract

Udang windu (Penaeus monodon Fabr.) merupakan komoditas asli Indonesia yang telah dibudidayakan cukup lama. Produksi udang windu pada 10 tahun terakhir diambil alih oleh udang vaname. Hal ini karena produksi dan produksifitas udang windu terus menurun 30,5% selama 5 tahun dari 180.000 ton (1995) menjadi 125.000 ton (2000). Penyebab utama penurunan produktifitas adalah wabah penyakit White Spot Syndrome Virus (WSSV). Penyakit WSSV dapat menyebabkan kematian masal dalam waktu singkat 6-11 hari pasca gejala klinis. Rancangan penelitian yang digunakan adalah Rancangan Acak Lengkap (RAL) dengan 5 perlakuan berbagai salinitas dengan 3 ulangan. Perlakuan salinitas terdiri dari A (10 ppt), B (15 ppt), C (20 ppt), D (25 ppt), dan E (30 ppt). Parameter penelitian yang diamati adalah gejala klinis, mortalitas, dan keberadaan virus WSS. Hewan percobaan yang digunakan adalah gelondongan udang dengan ukuran 2,5 ±0,14 g sebanyak 180 ekor. Infeksi WSSV dilakukan melalui perendaman 20 mg/ml WSSV selama 3 jam. Pengamatan dilakukan selama 21 hari. Hasil penelitian menunjukkan bahwa mortalitas tertinggi dan tercepat adalah perlakuan A dengan mortalitas 100% dalam waktu 6 hari dan tidak menunjukkan gejala klinis spesifik WSSV. Demikian pula perlakuan B dan C menglami mortalitas 100% dan juga tidak ditemukan gejala klinis spesifik WSSV. Gejala klinis spesifik WSSV ditemukan pada perlakuan D dihari ke-9 dengan mortalitas 43,33%. Uji PCR menunjukkan positif WSSV pada perlakuan A, B, C, D dan negatif WSSV pada perlakuan E. Gejala klinis udang windu yang terinfeksi WSSV adalah udang mendekati aerasi, lemah, tidak responsif, penurunan respon pakan, hepatopankreas pucat, tubuh kemerahan, berenang miring hingga berputar, dan bintik putih. Berdasarkan hasil penelitian tersebut dapat disimpulkan bahwa pada salinitas 30 ppt infeksi WSSV pada udang windu lebih rendah dibandingkan salinitas 10 ppt, 15 ppt, 20 ppt, dan 25 ppt. Tiger shrimp (Penaeus monodon Fabr.) is an endogenous species that has been traditionaly cultured by Indonesian fisherman. Production of tiger shrimp in the last 10 years were replaced by white shrimp (Litopenaeus vannamei). This disease has caused several decline shrimp productivity around 30,5% from 180.000 tonnes in 1995 to only 125.000 tonnes in 2000. It was due to disease outbreaks cause by White Spot Syndrome Virus (WSSV). WSSV could cause high mortality within 6-11 days after clinical sign was shown. This experimental method used RAL with 5 salinity treatments and 3 replications. Salinity treatments were A (10 ppt), B (15 ppt), C (20 ppt), D (25 ppt), and E (30 ppt). This research was aimed to find out the effect of various salinities concentration to the clinical sign, mortality, and the presence of WSSV in the tested shrimp. The animal tested were 180 tiger shrimps with 2,5 ±0,14 g weight. WSSV infection was done by submerging the tiger shrimp in 20 μg/ml WSSV for 3 hours. The observation was done for 21 days. The result showed the fastest and highest mortality was found in treatment A with 100% mortality in 6 days and without spesific sign of WSSV. While in threatment B and C showed same with 100% mortality and without clinical sign of WSSV. The spesific clinical sign of WSSV was showed in treatment D in day 9 with 43,33% mortality. The PCR test showed positive WSSV in treatment A, B, C, D and negative WSSV in treatment E. The clinical signs of WSSV infected tiger shrimp were approaching aeration, weak, unresponsive, decreased feeding response, pale hepatopankreas, reddish body, whirling, and white spots. The conclusion was in 30 ppt salinity the infection of WSSV in tiger shrimp was lower than the other salinity treatments (10 ppt, 15 ppt, 20 ppt, and 25 ppt).
PENGARUH DOSIS FITASE DALAM PAKAN BUATAN TERHADAP PERTUMBUHAN DAN EFISIENSI PEMANFAATAN PAKAN BENIH NILA LARASATI (Oreochromis niloticus) Restianti, Anggi; Rachmawati, Diana; Samidjan, Istiyanto
Journal of Aquaculture Management and Technology Volume 5, Nomor 2, Tahun 2016
Publisher : Journal of Aquaculture Management and Technology

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Abstract

Penggunaan bahan nabati sebagai sumber protein nabati dalam pakan memiliki kelemahan utama, seperti adanya asam fitat yang menyebabkan mineral-mineral penting dan protein dalam pakan tidak dapat diserap dengan baik oleh tubuh, sehingga efisiensi pemanfaatan pakan tidak maksimal. Penanggulangannya dilakukan dengan cara penambahan enzim eksogenus seperti enzim fitase ke dalam pakan buatan. Enzim fitase akan menghidrolisis asam fitat menjadi inositol dan asam fosfat, sehingga efisiensi pemanfaatan pakan, kecernaan dan penyerapan nutrisi menjadi maksimal. Tujuan dari penelitian ini adalah mengetahui pengaruh perbedaan dosis enzim fitase pada pakan buatan terhadap pertumbuhan, efisiensi pemanfaatan pakan dan kelulushidupan benih nila larasati (O. niloticus). Ikan uji yang digunakan adalah benih nila larasati (O. niloticus) dengan bobot rata-rata 0,72±0,05 g/ekor dan padat tebar 25 ekor/m3. Penelitian ini menggunakan metode eksperimental dengan Rancangan Acak Lengkap 4 perlakuan dan 3 kali ulangan. Perlakuan dalam penelitian adalah penambahan enzim fitase A (0 mg/kg pakan), B (400 mg/kg pakan), C (800 mg/kg pakan) dan D (1.200 mg/kg pakan). Hasil penelitian menunjukkan bahwa perlakuan D (1.200 mg/kg pakan) memberikan nilai RGR, EPP dan PER tertinggi yaitu 12,16±0,14% (RGR), 68,38±2,24% (EPP) dan 2,25±0,07 (PER). Kesimpulan yang didapat dari penelitian ini adalah pengaruh dosis fitase dalam pakan buatan memberikan pengaruh sangat nyata (P<0,01) terhadap RGR, EPP dan FCR dan berpengaruh nyata (P<0,05) terhadap PER, namun tidak berpengaruh nyata (P>0,05) terhadap SR. Dosis optimal yang dapat meningkatkan pertumbuhan, efisiensi pemanfaatan pakan dan rasio efisiensi protein adalah 1.200 mg/kg pakan yang mampu menghasilkan RGR sebesar 12,2%/hari, EPP sebesar 68,4% dan PER sebesar 2,25%. Kualitas air pada media pemeliharaan berada pada kisaran yang sesuai untuk budidaya ikan nila larasati. The use of plant material as a source of protein in artificial feed currently has major weakness, such as the presence of phytic acid that causesthe essential minerals and protein in the diet can not be absorbed by the body, so the efficiency of feed utilization was not optimal.Countermeasures is done by the addition of exogenous enzymes such as phytase enzyme into artificial feed. Phytase enzyme will hydrolyze phytic acid into inositol and phosphoric acid, so that the absorption of nutrients, feed utilization efficiency and a maximum digestibility. The purpose of this study was to determine the effect of different doses of phytase enzyme on artificial feed and determine the optimal dose of enzyme phytase on artificial feed to growth, efficiency of feed utilization and survival rate of larasati tilapia (O. niloticus). The fish sample which are used werewith an average weight of 0.72 ± 0.05 g/fish and stocking density 25 fish/m3. This research used experimental methodof completely randomized design with 4 treatments and 3 repetitions. The treatment in this study is addition of phytase enzime A (0 mg/kg feed), B (400 mg/kg feed), C (800 mg/kg feed) and D (1,200 mg/kg feed). The results showed that treatment D (1,200 mg/kg feed) value highest of RGR, EPP dan PER is 12.16 ± 0.14% (RGR), 68 , 38 ± 2.24% (EPP) dan 2.25 ± 0.07 (PER). The conclusion of this study is the effect of phytase dose in artificial diet provides highly significant effect (P<0,01) to RGR and EPP and significantly (P <0.05) of the PER. The optimal dose that can promote the growth, efficiency of feed utilization and protein efficiency ratio was 1.200 mg / kg of feed are capable of producing RGR 12.2%/day, EPP amounted to 68.4% and a PER of 2.25%. Maintenance of water quality in the media is in the range that is suitable for the cultivation of tilapia larasati.

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