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BIOTROPIA - The Southeast Asian Journal of Tropical Biology
ISSN : 02156334     EISSN : 1907770X     DOI : http://dx.doi.org/10.11598/btb
BIOTROPIA, The Southeast Asian Journal of Tropical Biology, is a scientific publication of the Southeast Asian Ministers of Education Organization (SEAMEO) – Southeast Asian Regional Center for Tropical Biology (BIOTROP). It publishes articles in the broad areas of tropical ecosystems and environmental impacts, biodiversity conservation and sustainable development and biotechnology.
Articles 520 Documents
Production of Maltooligosaccharides From Hutan Jati Variety Cultivar Tacca (Tacca leontopetaloides) Starch Yopi, Yopi; Rahmani, Nanik; Putri, Feby Heryani; Martin, Andri Fadillah
BIOTROPIA Vol. 26 No. 2 (2019): BIOTROPIA Vol. 26 No. 2 August 2019
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (224.311 KB) | DOI: 10.11598/btb.2019.26.2.890

Abstract

This research aimed to extract and characterize the physicochemical properties of starch from Tacca tuber, to determine the optimum conditions for enzymatic hydrolysis to produce maltooligosaccharides, and to analyze the character of these maltooligosaccharides. The analysis was conducted by calculating the amount of reducing sugar, total sugar, and the degree of polymerization, and by using the TLC (Thin Layer Chromatography) and HPLC (High-Performance Liquid Chromatography) analyses. The Hutan Jati variety cultivar of Tacca was selected from three Tacca variety cultivars (Hutan Jati, Pulau Katang, and Gunung Batur) to produce maltooligosaccharides by enzymatic hydrolysis of crude Bacillus sp. α-amylase. The optimum conditions for the enzymatic hydrolysis of Hutan Jati variety cultivar Tacca starch for the production of maltooligosaccharides were obtained at a substrate concentration of 3% (w/v) and a ratio of enzyme and substrate at 6 hours incubation time. From 250 mL of fresh hydrolysate, 34.49 grams of powdered maltooligosaccharide were produced. The TLC and HPLC results showed a similar yield of both the liquid and powdered maltooligosaccharides with maltose, maltotriose, and maltotetraose as the main products. Considering its physicochemical characteristics and the product of its maltooligosaccharides, the starch from the tuber of Hutan Jati variety cultivar Tacca possessed strong potential for the future production of maltooligosaccharides, particularly maltotriose and maltotetraose, in food industries.
THE EFFECTIVENESS OF SOME ECTOMYCORRHIZAL FUNGI IN ALGINATE BEADS IN PROMOTING THE GROWTH OF SEVERAL DIPTEROCARP SEEDLINGS SUPRIYANTO, SUPRIYANTO
BIOTROPIA No. 12 (1999)
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (670.122 KB) | DOI: 10.11598/btb.1999.0.12.148

Abstract

The effectiveness of six species of ectomycorrhizal fungi (Scleroderma columnare, S.dictyosporum, Laccaria toccata, Rhizopogon luteolus, Amanita umbronata and Descomyces sp.) in alginate beads in promoting the growth of four species of dipterocarp seedlings (Shorea pinanga, S.leprosula, S.ovalis and Hopea odorata) were studied. Different sodium alginate concentrations of 5 g, 10 g, 15 g and 20 g/L were tested to find out the best bead's elasticity and spore germination. Seedling height, Relative Field Mycorrhizal Dependency, percentage of mycorrhizal colonization, nutrient absorption, and the formation of Hartig's net and mantle structure of dipterocarp seedlings were observed 4 months after inoculation. The best elasticity of alginate beads was found in the concentration of sodium alginate of 15 g/L. The best growth increment was found in Hopea odorata inoculated with Amanita umbronata (126.60 %) followed by Shorea pinanga inoculated with Descomyces sp. (27.10%), Shorea ovalis inoculated with Amanita umbronata (26.90 %) and Shorea leprosula inoculated with Descomyces sp. (24.20 %) over the control. The highest Relative Field Mycorrhizal Dependency was found in Hopea odorata followed by Shorea ovalis, S. pinanga and S. leprosula. The highest mycorrhizal colonization was obtained in Shorea pinanga inoculated with Descomyces sp. (75%), while inoculation with Amanita umbronata on S.leprosula, S.ovalis and Hopea odorata increased mycorrhizal colonization i.e. 64.5 %, 52.5%, and 46.2 %, respectively. Hartig's net and mantle structures were well formed in Shorea leprosula as well as S.ovalis seedlings with all mycorrhizal fungi tested, while in S.pinanga seedlings these structures were only well formed with Descomyces sp. There is no clear difference in P levels in the leaves following inoculation as compared to the controls. Key words: Mycorrhizas/Alginate  beads/Scleroderma columnare/Scleroderma dictyosporumlLaccaria laccatalRhizopogon luteoluslAmanita umbronatalDescomyces  sp./Growth/Dipterocarpace-ae/Seedlings.
RAPID DETECTION OF THE AFRICANIZED HONEY BEE: A TOOL FOR INDONESIAN ANIMAL QUARANTINE RAFFIUDIN, RIKA; BINTAR, ANIFA; WIDJAJA, M. CHANDRA; FARAJALLAH, AHMAD; PURWANTARA, BAMBANG
BIOTROPIA Vol. 16 No. 1 (2009): BIOTROPIA Vol. 16 No. 1 June 2009
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Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (194.086 KB) | DOI: 10.11598/btb.2009.16.1.65

Abstract

Molecular detection methods were used to determine if Africanized Honey Bees (AHBs) are present in populations of imported Apis mellifera in Indonesia. The cytochrome b (cyt b gene) was amplified from mitochondrial DNA and digested with the BglII restriction enzyme (cytb/BglII). Two types of animal DNA extraction kits were used and found suitable for rapid preparation of DNA from A. mellifera by the Animal Quarantine facility. Results showed that all 94 colony samples from beekeepers in Java produced a 485 bp PCR product from the amplification of this gene. Two DNA fragments of 194 and 291 bp from all samples were produced after digestion with BglII. This cytb/BglII result together with the DNA sequence of cyt b showed that all collected samples of A. mellifera were the non-AHB type. Hence, this study did not detect AHB in Indonesia.Key words :  Apis mellifera, molecular detection methods, DNA, cytochrome b, mitochondrial genetics
PRODUCTION AND UTILIZATION OF CELLULASE FROM TRICHODERMA VIRIDE SURYAHADI SURYAHADI; R. HIDAYAT; S. WULANDARI; K.G. WIRYAWAN
BIOTROPIA - The Southeast Asian Journal of Tropical Biology No. 25 (2005)
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (96.263 KB) | DOI: 10.11598/btb.2005.0.25.208

Abstract

  An appropriate preservation technology for forage such as silage needs to be developed in order to overcome the shortage of tropical forage during the dry season. A good quality of silage is obtained by decreasing the fibre contents (Neutral Detergent Fibre, Acid Detergent Fibre and lignin). The research was conducted in two stages: 1). Production and activity test of crude enzymes from Trichoderma viride and 2). Comparative test using crude enzymes from  Trichoderma viride strain QM 9414 (CETV) and commercial cellulase (Cellulase “Onozuka R-10”, heidelberg) on rice straw silage. The treatments were arranged as follows : P0=Untreated rice straw silage, P1=Treated with commercial cellulase at 10 IU kg-1, P2= Treated with CETV at 3.8 IU kg-1, P3=Treated with CETV at 7.6 IU kg-1  and P4= Treated with CETV at 11.4 IU kg-1 of fresh rice straw. All treatments were enriched with 5% molasses. The result of the research indicated that: 1). The activity of crude enzymes from Trichoderma viride strain QM 9414 (CETV) was 1.52 IUml-1. 2). The addition of both enzymes did not decrease dry matter of rice straw silage; however, organic matter significantly decreased (p
Effect of Culture Parameters on Protease and Cellulase Production by Two Bacterial Strains, Corynebacterium alkanolyticum ATH3 and Bacillus licheniformis CBH7 Isolated From Fish Gut Banerjee, Goutam; Nandi, Ankita; Ray, Arun Kumar
BIOTROPIA Vol. 24 No. 3 (2017): BIOTROPIA Vol. 24 No. 3 December 2017
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (904.899 KB) | DOI: 10.11598/btb.2017.24.3.684

Abstract

Microbial protease and cellulase are in high demand by different industries due to their minimal cost and availability. This study was aimed to maximize the production of protease and cellulase using two bacteria, Corynebacterium alkanolyticum ATH3 and Bacillus licheniformis CBH7, isolated from fish gut. This study demonstrated the effect of different culture parameters in protease and cellulase production using two different bacterial strains. Results of this study clearly indicated the importance of different parameters such as moisture content, pH, incubation temperature, incubation period, inoculum size, carbon sources and nitrogen sources in enzyme production. The most critical parameters affecting the enzymes production were pH, temperature, carbon and nitrogen sources. Further investigations are required to enhance the enzymes production using genetic engineering.
NOTES ON THE FAMILY AMPULLARIIDAE (GASTROPODA: PROSOBRANCHIA) IN THE PHILIPPINES: I. DIGESTIVE, CIRCULATORY, AND EXCRETORY SYSTEMS PAOULAYAN, ROBERTO C
BIOTROPIA No. 6 (1992)
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Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1884.512 KB) | DOI: 10.11598/btb.1992.0.6.104

Abstract

A total of 232 ampullariid snails collected from 23 sites covering 7 islands in the Philippines were compared conchologically and 200 alcohol-preserved specimens were dissected for anatomical characteristics. Conchological comparison of the shells of the collected snails with that of identified lots from the Senckenberg Natur-museum, Frankfurt, Germany, the British Museum for Natural History, London, England, the Koninklijk Belgisch Instituut voor Natuurwetenschappen, Brussels, Belgium, and the Rijksmuseum voor Natuurwetenschappen, Leiden, Netherlands, revealed the presence of 5 species in the collected samples. These are: P. conica, P. ampullacea, P. mainitensis, P. quadrasi, and P. vittala. The latter 3 species were previously reported as being indigenous to the Philippines. Aside from characteristics of the shell, the morphology of the stomach may be useful for species discrimination. P. quadrasi and P. vittata, however, do not seem to differ anatomically from P. conica.
Effect of Substrate Moisture Content and Invasive Grass Competition on Native Fig (Ficus fistulosa) Seedling Recruitment in Limestone Quarries Satyanti, Annisa
BIOTROPIA Vol. 22 No. 2 (2015): BIOTROPIA Vol. 22 No. 2 December 2015
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (386.107 KB) | DOI: 10.11598/btb.2015.22.2.367

Abstract

This study was conducted to assess the potential use of the native fig (Ficus fistulosa) for rehabilitating degraded habitats in limestone quarry areas. A greenhouse experiment was performed to evaluate the effects of different substrate moisture levels and competition between the native fig (Ficus fistulosa) and the invasive grass Pennisetum polystachyon on the growth and survival of the native fig. Ficus fistulosa was selected as the study species because it exhibited the highest Importance Value Index (IVI) among other fig species recorded in the Ciampea limestone hill. The results showed that substrate moisture levels did not significantly affect fig biomass, and invasive grass biomass was not reduced under drought stress. However, the interaction between substrate moisture levels and competition with the invasive grass significantly reduced overall fig biomass, although it did not affect leaf number or individual plant size. This study suggests that successful quarry rehabilitation efforts should include invasive species management alongside soil treatment interventions.
INCREASING RISK OF HEAVY METAL CONTAMINATION IN SILVOFISHERY PONDS Endah Dwi Hastuti
BIOTROPIA - The Southeast Asian Journal of Tropical Biology Vol. 28 No. 1 (2021): BIOTROPIA Vol. 28 No. 1 April 2021
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (522.6 KB) | DOI: 10.11598/btb.0.0.0.983

Abstract

This research aims to observe the concentration of organic matter, Pb and Cd in a silvofishery pond, to study the toxicity level status, to analyze changes in their concentration within a year’s period, and to analyze the correlation between the concentration and changes. The research was conducted through field observation and laboratory analysis from May 2016 to July 2017, which included five observation activities. Statistical analysis was conducted by using ANOVA and correlation tests. The results show that the concentration of organic matter, Pb and Cd, was increasing in all five observations. Throughout the research, the ranges of organic matter, Pb and Cd, were recorded at 1.60–3.30 mg/kg, 3.130–8.230 mg/kg, and 1.089–2.820 mg/kg, respectively. In all observations, toxicity level showed that Cd concentration in the sediment had exceeded the standards recommended by US EPA (≤1.0 mg/kg) and ANZECC & ARMCANZ (≤1.5 mg/kg), while Pb was within the safe range (≤21 mg/kg and ≤50 mg/kg). The correlation analysis showed that the concentration and accumulation of Pb and Cd were highly related, which indicated the possibility of the same pollutant sources. Recommendations for a better management plan to avoid heavy metal accumulation in silvofishery ponds would include the arrangement of mangrove plants in inlet canals and periodic pruning to hinder heavy metal from returning to the environment through litter fall.
Identification of Secretory Structure, Histochemistry and Phytochemical Compounds of Medicinal Plant Hyptis sapitata Jacq. Rupa, Darius; Sulistyaningsih, Yohana Caecilia; Dorly, Dorly; Ratnadewi, Diah
BIOTROPIA Vol. 24 No. 2 (2017): BIOTROPIA Vol. 24 No. 2 August 2017
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Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11598/btb.2017.24.2.499

Abstract

Hyptis capitata Jacq. (common name: Knobweed or False Ironwort) belongs to Lamiaceae family and is among known traditional medicine. The Anak Dalam Tribe of Jambi Province uses the leaves of H. capitata to cure external and internal wounds. This study was aimed at identifying and analyzing secretory structure, histochemistry and phytochemical content of H. capitata leaves.  The results showed that H. capitata leaves have secretory structures in the form of peltate, capitate and uniseriate glandular trichomes on the upper and lower leaf surfaces, with idioblast cells scattered throughout the leaf mesophyll. Histochemical tests indicated that the peltate trichomes have four head cells, containing alkaloids and terpenoids. This study classified capitate trichomes into two types: 1. type I has one stalk cell and two head cells, all containing  alkaloids, terpenoids and lipophilic compounds; 2. type II has long stalk cells consisting of seven to ten cells with one head cell, all containing alkaloids and terpenoids. Uniseriate glandular trichomes of H. capitata leaves consist of four to eight cells containing alkaloids and terpenoids. The idioblast cells are round-shaped and contain lipophilic compounds. GC-MS analysis showed that H. capitata leaves  contain terpenoid compounds assumed to serve as anti-infective agents, including l-limonene, eugenol, farnesol isomers A, d-nerolidol, hexahydrofarnesol and neophytadiene.
Modelling Natural Mortality of Tropical Plantation Species Acacia mangium Willd Krisnawati, Haruni
BIOTROPIA Vol. 25 No. 3 (2018): BIOTROPIA Vol. 25 No. 3 December 2018
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (289.558 KB) | DOI: 10.11598/btb.2018.25.3.835

Abstract

Natural mortality of trees is extremely variable due to the uncertainty and complexity of the functioning of forest ecosystems. The objective of this study was to develop a stand-level mortality model for Acacia mangium species by relating mortality to stand variables that affect the natural mortality process. The model was developed using data from l97 permanent sample plots measured periodically at 1-yr time intervals from 2-4 years until 8-11 years after planting in South Sumatra, Indonesia. The model consists of two complementary equations. The first equation is a logistic function predicting the probability of mortality incidence depending on stand density, site index and stand age. The second equation estimates the reduction in the number of surviving stems observed in a stand where natural mortality occurs. Nine equations were fitted using data from permanent sample plots where trees died over the time period and the best model was selected. Estimates from this second model were then adjusted by a factor equal to the probability of mortality applying three different approaches: probabilistic two-step, deterministic threshold and stochastic. All methods revealed no significant difference between the observed and the predicted number of surviving stems per ha. The probabilistic two-step approach, however, produced more consistent and the most accurate estimates. This method should provide reliable prediction when it is to be used in forest productivity prediction and management system for the species.

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