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The Journal of Experimental Life Sciences (JELS)

jels
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Published by Universitas Brawijaya

ISSN : 20872852 EISSN : 23381655 DOI : 10.21776/ub.jels

Core Subject : Health, Science,

Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology Neuroscience

The Journal of Experimental Life Science (JELS) is a scientific journal published by Postgraduate School, University of Brawijaya as distribution media of Indonesian researcher’s results in life science to the wider community. JELS is published in every four months. JELS published scientific papers in review, short report, and articles in Life Sciences especially biology, biotechnology, nanobiology, molecular biology, botany, microbiology, genetics, neuroscience, pharmacology, toxicology, and Applied Life Science including fermentation technology, food science, immunotherapy, proteomics and other fields related to life matter. JELS is a scientific journal that published compatible qualified articles to the academic standard, scientific and all articles reviewed by the expert in their field. The Journal of Experimental Life Science (JELS) have a vision to become qualified reference media to publish the best and original research results and become the foundation of science development through invention and innovation on cellular, molecular, nanobiology, and simulation work related to life matter rapidly to the community. The Journal of Experimental Life Science (JELS) has objectives to published qualified articles on research’s results of Indonesian researchers in life science scope. JELS encompasses articles which discuss basic principles on natural phenomenon with cellular, molecular, and nanobiology approach.

Arjuna Subject : Biokimia, Genetika dan Biologi Molekuler - Biokimia, Genetika dan Biologi Molekuler (Lain-Lain)

Articles 299 Documents

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Efek Proteksi Kombinasi Minyak Wijen (Sesame Oil) dengan α-Tocopherol terhadap Steatosis melalui Penghambatan Stres Oksidatif pada Tikus Hiperkolesterolemia Nur Khoma Fatmawati; Mulyohadi Ali; Edi Widjajanto
The Journal of Experimental Life Science Vol. 2 No. 2 (2012)
Publisher : Postgraduate School, Universitas Brawijaya

Minyak wijen (MW) yang banyak mengandung polyunsaturated fatty acid (PUFA) berfungsi menurunkan kadar lipid serum melalui induksi β oksidasi di mitokondria. Proses ini menghasilkan produk sampingan berupa radikal bebas. Vitamin E (α-tocopherol) diketahui menghambat aktifitas radikal bebas. Penelitian ini bertujuan untuk membuktikan efek sinergisme MW dan α-tocopherol dalam menghambat steatosis yang diakibatkan keadaan hiperkolesterol. Hasil penelitian menunjukan bahwa diperoleh perbedaan bermakna (p<0,05) terutama dosis MW 1,2 ml pada semua parameter antara kelompok hiperkolesterol, MW dan MW+α-tocopherol. Kadar kolesterol kelompok hiperkolesterol (140,925±10,5) mg/dl; kelompok MW (93,845±4,37) mg/dl dan kelompok MW+α-tocopherol (92,90±8,5) mg/dl. Kadar trigliserida kelompok hiperkolesterol (154,78±11,09) mg/dl; kelompok MW (184,64±3,87) mg/dl dan kelompok MW+α-tochopherol (66,89 ± 6,03) mg/dl. Pada kelompok MW kadar MDA (0,257±0,013) nmol/mg protein, kelompok hiperkolesterol (0,847±0,036) nmol/mg protein dan kelompok kombinasi MW dengan α-tocopherol (0,092±0,006) nmol/mg protein. Kadar SOD pada kelompok kombinasi MW dengan α-tocopherol (253,82±16,63) U/mg protein, kelompok MW (208,7±11,27) U/mg protein, kelompok hiperkolesterol (139,7±2,82) U/mg protein. Dari gambaran histologis steatosis lebih banyak didapatkan pada kelompok hiperkolesterol yang disertai dengan keradangan, sedangkan pada kelompok kombinasi minyak wijen dengan α-tochopherol memiliki gambaran histologis terendah mengalami steatosis. Kata kunci: hiperkolesterol, minyak wijen, steatosis

Effect of Calcium Polysulfide Applications on Severity of Cocoa Pod Rot (Phytophthora palmivora Butl.) and Fungal Diversity Wahyu Widiyasmoro; Mintarto Martosudiro; Liliek Sulistyowati
The Journal of Experimental Life Science Vol. 7 No. 2 (2017)
Publisher : Postgraduate School, Universitas Brawijaya

Indonesian government gives a high priority for the development and revitalization of cocoa production. In 2012/2013, production of cocoa in Indonesia reached 410,000 ton from 1,774,500 ha (about 231 kg ha-1), lower than Ghana which is 835,000 ton from 1,600,300 ha (about 521 kg ha-1). One of the constraints of cocoa production in Indonesia is pod rot disease caused by Phytophthora palmivora, which can reduce about 90% of production during wet season. Calcium polysulfide was suggested as one of the potential, cheap and save substance to control P. palmivora. The purpose of this study was to evaluate the calcium polysulfide potential to control the disease and its effect on the phyllosphere-fungal diversity. A positive correlation was found between concentration of calcium polysulfide and inhibition of fungal growth in the media. Otherwise, a negative correlation was found between concentration of calcium polysulfide and fungal biomass. Disease severity of cocoa treated by calcium polysulfide 80 mL L-1 every weekÂ was 11.67% significantly lower than control, i.e. 68.33%. Furthermore, it was also found that the index value of fungal diversity on the phylosphere decreased by increasing concentration and interval ofÂ calcium polysulfide application.Keywords: Calcium polysulfide, Cocoa, Phytophthora palmivora.

Heavy Metal (Pb) and Its Bioaccumulation in Red Algae (Gracilaria sp.) At Kupang Village, Jabon Sub-District, Sidoarjo District Yatris Rambu Tega; Endang Yuli Herawati; Yuni Kilawati
The Journal of Experimental Life Science Vol. 9 No. 2 (2019)
Publisher : Postgraduate School, Universitas Brawijaya

Seaweeds have an inherent capacity to absorb heavy metals from marine water. This intrinsic ability allows these organisms to accumulate much amount of heavy metals over time. This study conducted at Kupang Village, Jabon Sub-District, Sidoarjo District, where almost 40% of the area consists of Gracilaria sp. ponds cultured. The purpose of this study was to analyze the Pb heavy metals concentration in water culture and its concentration in holdfast and thallus of Gracilaria sp. In this research, determination of samples in each pond was taken on day 0 (before planting), 20 days (Initial Production) and on day 40th (Post/Harvest). The aquatic parameters include salinity, temperature, acidity (pH), and dissolved oxygen (DO). Quantitative determination of heavy metals on sample using Atomic Absorption Spectrophotometry (AAS). Determinations of heavy metal accumulation in organism using Bioconcentratin factor (BCF) and Translocation factor was used to calculate the Pb heavy metal translocation process from the base to the tip of Gracilaria sp. The results of the study showed the highest Pb concentration found in the second sampling age 20 days, in pond 1 with 7.61 ± 0.18 mg.kg-1, and pond 2 was 5.35 ± 0.09 mg.kg-1. This concentration has not exceeded the threshold value that might have an effect if more than 8.6 mg.kg-1. The highest Pb level at the holdfast of Gracilaria sp. found at age 0 days before planting, which is 3.38 ± 0.23 mg.kg-1 and decreases to post-harvest (age of 40 days) which is 0.84 ± 0.00 mg.kg-1. The Transaction Factor (TF) of Pb heavy metal value from holdfast to thallus is 1,015 thus Gracilaria sp. absorbs heavy metals in high concentrations at the beginning of planting and is able to release it again before harvest time. Keywords: Bioaccumulation, Gracillaria sp., Heavy Metal, Histological, Red Algae.

Peran Puerarin terhadap Aktivitas Intra dan Ekstraseluler pada Kultur Human Umbilical Vein Endothelial Cells (HUVECs) Pasca Induksi Leptin Mochamad Sasmito Djati; Satuman Satuman; Retty Ratnawati; Sri Widyarti; Erly Nur Aisyah; Noer Hasanah; Eko Puji Astuti; Ririn Rochmawati
The Journal of Experimental Life Science Vol. 1 No. 1 (2011)
Publisher : Postgraduate School, Universitas Brawijaya

Beberapa penelitian terkini menyebutkan bahwa leptin merupakan salah satu penyebab disfungsi endotel yang merupakan salah satu penyebab aterogenesis. Antioksidan puerarin diduga memiliki kemampuan untuk mencegah mekanisme aterogenesis yang distimulasi oleh beberapa sitokin. Berdasarkan hal tersebut, maka tujuan penelitian ini adalah membuktikan dan mengetahui potensi puerarin untuk menghambat ekspresi dan aktivitas intra dan ekstraseluler VCAM-1, PPAR-ɣ, SOD dan H2O2, apoptosis dan nekrosis pada kultur Human Umbilical Vein Endothelial Cells (HUVECs) yang diinduksi 25 ng ml-1 leptin. Penelitian ini mempergunakan sel kultur primer HUVECs yang dibagi menjadi empat kelompok perlakuan, yaitu kelompok 0 ng ml-1 dan 0 µM puerarin, kelompok sel yang diinduksi 25 ng ml-1 leptin selama 12 jam, kelompok induksi puerarin 5, 25, 200 dan 525 µM puerarin selama enam jam tanpa leptin, kelompok induksi leptin dan puerarin dengan konsentrasi 5, 25, 200 dan 525 µM selama enam jam. Aktivitas VCAM-1 dan PPAR-ɣ diketahui dengan analisis imunositokimia, metode ELISA digunakan untuk analisis aktivitas SOD dan H2O2. Apoptosis dan nekrosis sel dianalisis setelah HUVECs diberi penanda BrdU selama 20 jam. Data dianalisis dengan analisis satu jalur (ANOVA) dan dilanjutkan dengan uji Tukey. Hasil penelitian menunjukkan bahwa induksi 25 ng ml-1 dapat meningkatkan ekspresi VCAM-1 (2,68 ± 0,15)% dibandingkan dengan perlakuan 0 ng ml-1 (0,54 ± 0,15)%. Perlakuan induksi puerarin 5, 25, 200, 525 μM memberikan dampak negatif terhadap ekspresi VCAM-1 meskipun pengaruh ini tidak signifikan. Puerarin dapat menekan apoptosis dan nekrosis sel, 525 μM puerarin secara efektif dapat menekan ekspresi PPAR-ɣ. Puerarin tidak memberikan dampak yang signifikan terhadap aktivitas ekstraseluler berdasarkan hasil analisis aktivitas SOD dan H2O2. Kata kunci: apoptosis, H2O2, HUVECs, nekrosis, leptin, puerarin, VCAM-1, PPAR-ɣ, SOD

Oocyte In Vitro Maturation with Crude Sperm Extract Protein of Bull's Spermatozoa Bilqis Bilqis; Sri Rahayu; Gatot Ciptadi
The Journal of Experimental Life Science Vol. 6 No. 1 (2016)
Publisher : Postgraduate School, Universitas Brawijaya

Oocyte In vitro maturation (IVM) is one of the important parts for in vitro fertilization (IVF). The success of oocyte maturation is influenced by the composit ion and the quality of IVM medium. Culture medium which used to IVM not only influences the oocyte process to reach metaphase II and proceed the fertilization, but also influences to developmental of an embryo. Crude sperm extract has high-level protein kinase and contains some sperm proteins. Crude sperm extracts expected as natural maturation medium that can increase the success of In Vitro Maturation (IVM). The characterization of crude sperm extracts profile with sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Oocyte maturation is observed in the 26th hour from the first culture. The result of crude sperm extract characterization obtained from the protein with the molecular weight is 176.8, 63.2, 58.4, 55.3, 52.4, 49.7, 44.6, 38.02, 36.03, 34.15 and 26.8 kDa. Oocyte maturation with concentration of crude sperm extract 1.5 µg.mL-1 with 71.6% matures oocyte and oocyte maturation with concentration of crude sperm extract 2.5 µg.mL-1 with 75% matures oocyte. Keywords: Crude Sperm Extract, In Vitro Maturation, Oocyte

Expression of Hsp70 and β-actin Genes as The Immune Response against Viral Nervous Necrosis that Infected Asian Seabass (Lates calcalifer) Yusuf Arif Wahyudi; Uun Yanuhar; Maftuch Maftuch
The Journal of Experimental Life Science Vol. 8 No. 3 (2018)
Publisher : Postgraduate School, Universitas Brawijaya

The viral nervous necrosis (VNN) is betanodavirus of the family Nodaviridae with acute infection and associated with high levels mortality up to 100% of numerous larval-stage marine and freshwater fish species. The danger signals of viral infection that are capable of activating APCs (Antigen Presenting Cells), furthermore produced molecules protein receptors such as interferon and heat shock proteins. Stimulating interferon type I (IFN I) induce several antiviral molecules, further binding the actin cytoskeleton to reach the site of infection. This study demonstrated a correlation between increased levels of Hsp70 (heat shock proteins) and actin filamentous (β-actin) within invasion wild-type isolate of ssRNA VNN from Asian Seabass (L. calcalifer) juvenile. Furthermore, expression of Hsp70 and actin as an indicator or biomarker of stressed states in fish. The reverse transcriptase polymerase chain reaction (RT-PCR) method used to finding expression Hsp70 and β-actin. Whereas nested RT-PCR used for VNN genes. The up-regulation of Hsp70 was observed on the brain tissue higher than eye tissue of sample positive infected VNN. Whereas different expression of β-actin, β-actin receptor expression tends to be stable in the organs of the brain and eye, both invaded VNN or normal, while on the eye, the ratio slightly increased based on the intensity of the band. The research shows that altered expression of heat shock protein 70 and beta-actin gene receptors in the target organs that response to invasion VNN at Asian seabass (L. calcalifer) juvenile. Alteration expression of heat shock protein 70 and beta-actin can be used as an indicator or biomarker of stress cells, especially from VNN invasion at fish. Keywords: Asian seabass, β-actin, Hsp70, Immune response, Viral nervous necrosis.

Identification of Coral Reefs in Mamburit Waters, Sumenep Regency Sawiya Sawiya; Mohammad Mahmudi; Guntur Guntur
The Journal of Experimental Life Science Vol. 4 No. 1 (2014)
Publisher : Postgraduate School, Universitas Brawijaya

This research was conducted in September to October 2013 in Mamburit Waters, Sumenep Regency. This study was aimed to assess the percentage of coral reefs and acknowkedge the type of the coral reefs. Coral reefs was observed with the Line Intercept (LIT) method laid parallel to the coastline in the depth of 3 m and 10 m in windward and leeward area. Total of 59.88% coral reefs lived in leeward area in 3 m depth includes in good category and the percentage of dead coral reefs and other fauna for 40.12%. In the 10 m depth, 69.19% live coral reefs include as medium category with percentage of dead coral reefs and other fauna for 30.81%. Winward location of 3 m depth has 68.38% live coral reefs in good category and percentage of dead coral reefs and other fauna for 31.62%. Whereas for 10 m depth, 40.86% of live coral reefs include as medium category with percentage of dead coral reefs and other fauna for 59.14 %. Keywords: coral reefs, leeward, windward, Mamburit

In Vitro Morphogenesis Responses of Various Explant in Physalis angulata L. Retno Mastuti; Aminatun Munawarti
The Journal of Experimental Life Science Vol. 7 No. 2 (2017)
Publisher : Postgraduate School, Universitas Brawijaya

The objective of this research was to identify morphogenesis responses of in vivo and in vitro-derived explant of Physalis angulata L. Explants were cultured on MS medium containing 3% sucrose solidified with 1.3% agar supplemented with combination of 6-benzylaminopurine (BAP), indole acetic acids (IAA), naphthalene acetic acid (NAA) and 2,4-dichlorophenoxyacetic acid (2,4-D). All explant types have morphogenesis ability. Capability for shoot regeneration was observed in high number of shoot tip (96.1%) and nodes explants (75.2%). Callus formation was dominantly produced by in vitro leaf explants. In vivo leaf had low responses for shoot/root regeneration and callus formation. These results provided an alternative choice of the explant suitable for either plant regeneration or callus formation.Keywords: callus, cotyledonary nodes, explant types, shoot regeneration.

The Response of Botanical Seeds of Trisula and Biru Lancor Shallot Varieties to Coconut Water Treatment Sudaryono, Tri
The Journal of Experimental Life Science Vol. 9 No. 3 (2019)
Publisher : Graduate School, Universitas Brawijaya

The use of botanical seeds for seedling cultivation has problems related to the number of produced tubers, where in general botanical seeds on average only produce 1-2 tubers. Another problem with the cultivation of shallots with botanical seeds as a source of seedlings is the low growth of seeds (germination). To overcome the problems that exist in botanical seeds, growth regulators are used. This study aimed to determine the effect of coconut water treatment on the growth and yield of Trisula and Biru Lancor shallot varieties from botanical seeds, carried out from April to August 2018 in Pelem Village in Pare Sub-District, Kediri Regency. The utilized method is soaking the shallot botanical seeds for 4 hours before sowing; the seeds were then sown and planted for a month before being moved to the field. After growing in the field, observations were carried out at 1-week intervals on vegetative and generative growth. Sampling was random, and data was analyzed with t-test/LSD at a level of 5%. The results showed that the botanical seeds of Trisula and Biru Lancor shallot varieties showed different responses to the treatment with young coconut water. The use of coconut water for the Trisula variety led to 70% of plants yielding more than six cloves, while the Biru Lancor variety only had 46.67%. The use of coconut water for the Trisula variety yielded a fresh tuber weight of 71.2 g per plant at harvest; if converted per hectare, the Trisula variety can yield approximately 32 tons of fresh tubers. Meanwhile, the Biru Lancor variety yielded a tuber weight of 57.4 g per plant at harvest, and if converted per hectare, the Biru Lancor variety can yield approximately 26 tons of fresh tubers.Keywords: Botanical seed, Coconut Water, Shallots

Pemberian Beberapa Kombinasi ZPT Terhadap Regenerasi Tanaman Gloxinia (Siningia speciosa) dari Eksplan Batang dan Daun Secara In Vitro Imelda Jeanette Lawalata
The Journal of Experimental Life Science Vol. 1 No. 2 (2011)
Publisher : Postgraduate School, Universitas Brawijaya

Beberapa kombinasi Zat Pengatur Tumbuh (ZPT) seperti auksin, Fulvic acid dan sitokinin dicobakan untuk memacu regenerasi tanaman gloxinia (Siningia speciosa) dari eksplan batang dan daun secara in vitro. Auksin terdiri dari NAA (0, 2.5, 5.0 mgl-1), fulvic acid (0 dan 1.0 mgl-1) serta sitokinin (BA 5 mgl-1, Novelgro 5 mll-1, air kelapa 20%). Setiap perlakuan diulang 10 kali. Hasil penelitian menunjukkan bahwa eksplan yang mendapat perlakuan BA 5 mgl-1 (M1) dan perlakuan sitokinin tanpa auksin (M1 â€“ M6) memberikan jumlah tunas yang lebih banyak dibandingkan perlakuan auksin. Jumlah daun tertinggi diperoleh pada Media 3 (NAA 0 mgl-1 + Fulvic acid 0 mgl-1 + Air kelapa 20%) dan Media 6 (NAA 0 mgl-1 + Fulvic acid 1 mgl-1 + Air kelapa 20%). Hampir semua perlakuan NAA dan Fulvic acid yang dikombinasikan dengan air kelapa menunjukkan pertumbuhan jumlah daun yang lebih tinggi dibandingkan kombinasi perlakuan lainnya.Kata kunci: auksin, fulvic acid, Gloxinia, Siningia speciosa, sitokinin

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Jehan Ramdani Hariyati

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Home Page

OAI Link

Editorial Team

Contact

Reviewer

Google Scholar

Contact Name Jehan Ramdani Hariyati

Contact Email jehanramdani@ub.ac.id

Phone +6282333752235

Journal Mail Official jehanramdani@ub.ac.id

Editorial Address Jl. Veteran Malang 65145

Location Kota malang, Jawa timur INDONESIA

Abstract

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Abstract

Contact Name
Jehan Ramdani Hariyati

Contact Email
jehanramdani@ub.ac.id

Phone
+6282333752235

Journal Mail Official
jehanramdani@ub.ac.id

Editorial Address
Jl. Veteran Malang 65145

Location
Kota malang,

Jawa timur

INDONESIA