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The Journal of Experimental Life Sciences (JELS)

jels
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Published by Universitas Brawijaya

ISSN : 20872852 EISSN : 23381655 DOI : 10.21776/ub.jels

Core Subject : Health, Science,

Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology Neuroscience

The Journal of Experimental Life Science (JELS) is a scientific journal published by Postgraduate School, University of Brawijaya as distribution media of Indonesian researcher’s results in life science to the wider community. JELS is published in every four months. JELS published scientific papers in review, short report, and articles in Life Sciences especially biology, biotechnology, nanobiology, molecular biology, botany, microbiology, genetics, neuroscience, pharmacology, toxicology, and Applied Life Science including fermentation technology, food science, immunotherapy, proteomics and other fields related to life matter. JELS is a scientific journal that published compatible qualified articles to the academic standard, scientific and all articles reviewed by the expert in their field. The Journal of Experimental Life Science (JELS) have a vision to become qualified reference media to publish the best and original research results and become the foundation of science development through invention and innovation on cellular, molecular, nanobiology, and simulation work related to life matter rapidly to the community. The Journal of Experimental Life Science (JELS) has objectives to published qualified articles on research’s results of Indonesian researchers in life science scope. JELS encompasses articles which discuss basic principles on natural phenomenon with cellular, molecular, and nanobiology approach.

Arjuna Subject : Biokimia, Genetika dan Biologi Molekuler - Biokimia, Genetika dan Biologi Molekuler (Lain-Lain)

Articles 299 Documents

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Aspek Biologi Sel T Regulator CD4+ CD25+ pada Transplantasi Sumsum Tulang Muhaimin Rifa'i
The Journal of Experimental Life Science Vol. 4 No. 1 (2014)
Publisher : Postgraduate School, Universitas Brawijaya

Graft-versus-host disease (GVHD) dan rejeksi (penolakan) pada transplantasi alogenik merupakan kasus terpenting pada masalah transplantasi jaringan. Di lain pihak ketersediaan donor dengan kriteria MHC-matching sangat terbatas. Fakta bahwa kebanyakan organisme hidup sehat tanpa terjangkit penyakit autoimun menunjukkan adanya mekanisme regulasi yang mampu mencegah teraktivasinya sel-sel autoreaktif. Organisme mempunyai mekanisme yang sangat sempurna untuk menghindari respon mematikan terhadap self-antigen. Mekanisme terpenting pada toleransi imunologi ini adalah yang diperankan oleh sel T regulator. Mekanisme toleransi yang dilakukan oleh sel T regulator mencegah teraktivasinya sel-sel autoreaktif ini nampaknya juga terjadi pada transplantasi, baik transplantasi sumsum tulang maupun organ. Pada review ini kami akan membahas aplikasi sel T regulator CD4+CD25+ pada transplantasi alogenik terutama pada transplantasi sumsum tulang (bone marrow transplantation, BMT). Kata kunci: sel T regulator, transplantasi sumsum tulang, toleransi imunologi

Exploration and Antagonistic Test of Endophytic Fungi from Soybean (Glycine max L. Merr) With Different Resistance to Sclerotium rolfsii Siti Aminatuz Zuhria; Syamsuddin Djauhari; Anton Muhibuddin
The Journal of Experimental Life Science Vol. 6 No. 2 (2016)
Publisher : Postgraduate School, Universitas Brawijaya

The research aimed to determine the diversity of endophytic fungi in soybean crop with different resistance against Sclerotium rolfsii and find out their potential antagonist in controlling S.rolfsii by in vitro and in vivo. Materials used in this study were soybean with a variety of Wilis (susceptible variety) and Sinabung (resistant variety). This research was conducted at the Microbiology Laboratory of Central Laboratory of Life Science (LSIH), Brawijya University and in the trial plantation of Malang Research Institute for Food Crops, Lawang, Malang subdistrict in September 2015 until May 2016. Type of experimental design used was Complete Randomized Design with 16 treatments and three times repetitions at in vitro experiment with 16 treatments and four times repetitions at in vivo experiment. Observation on in vitro test is covering to colony morphology of fungal pathogens on PDA medium. On the test of in vivo, it was observed a disease occurrence and effectiveness rate of endophytic fungi. There are 15 species of endophytic fungi produced from isolation, namely Trichoderma sp., Aspergillus sp.2, Aspergillus sp.3, Acremonium sp.1, Acremonium sp.2, Acremonium sp.3, Acremonium sp.4, Fusarium sp.1, Fusarium sp.2, Cephalosporium sp, Microsporum sp., Penicillium sp., and unidentified fungi called W2 and W4. The highest inhibitory of endophytic fungi against S. rolfsii by Aspergillus sp.2 is 89.18% (in vitro) and 61.21% (in vivo), while Trichoderma sp. 91.88% (in vitro) and 63.29% (in vivo). Diversity index value of Wilis variety is higher than Sinabung, i.e. 1.878 and 1.606 respectively. While dominance index value of Sinabung variety is 0.2035 and Wilis is 0.1528. Keywords: Endophytic fungi, diversity, S. rolfsii.

Phytoremediation of Lead-Contaminated Soil by Using Vetiver Grass (Vetiveria zizanioides L.) Rawidh Said Abdallah; Arief Rachmansyah; Bagyo Yanuwiadi
The Journal of Experimental Life Science Vol. 9 No. 1 (2019)
Publisher : Postgraduate School, Universitas Brawijaya

Lead become a frequent problem in the environment, especially in the soil. However, there are plenty of plant species that can be used to overcome that problem. This study was aimed to analyze the effectiveness of Vetiver grass to reduce hyper-accumulate heavy metal such as lead (Pb) in the soil. Moreover, this study focused on the determination of heavy metals in the soil before plantation and after plantation of Vetiver. The method used was by planting the Vetiver grass in soil that was already treated by lead nitrate, lead chloride, and lead sulfate. Samples were collected from these treated soil every two weeks and samples of the vetiver grass were taken after one week for ten weeks. The result shows that Vetiver grasses are able to remove hyper-accumulate lead from the treated soil.Key words: Heavy metals, hyper accumulator, Vetiver grass.

The Potential of EMSA Eritin to Modulate T Cells (CD4+ and CD8+) in Balb/C Mice Model of Diabetes Mellitus Ade MC Rohie; Muhaimin Rifa'i
The Journal of Experimental Life Science Vol. 5 No. 1 (2015)
Publisher : Postgraduate School, Universitas Brawijaya

EMSA Eritin is a medicinal polyherbal contains with soy bean, coconut water and red rice extract. In several studies of this individual components have proven the potential to modulate lymphocyte cells that indirectly lowering blood glucose levels in diabetes meliitus. This study was aimed to analyzing the effect of polyherbal EMSA Eritin on the expression of CD4+, CD8+ and SDF-1 cells population in mice model of diabetes mellitus. Neonatal d-5 mice was intraperitoneally injected with Streptozotocin (STZ) 100 mg.kg-1 BW. Four weeks post injection the mice was treated with EMSA Eritin in three doses 0.3125 mg.g-1 BW, 3.125 mg.g-1 BW and 31.25 mg.g-1 BW for 14 days. Cells Profile of T lymphocyte (CD4+ and CD8+) and chemokine SDF-1 were analyzed by Flow cytometry. Data were analyzed with One-way ANOVA (p<0.05) and Duncan test using SPSS 16.0 for Windows. The result showed that EMSA Eritin significantly increased T lymphocyte (CD4+ and CD8+) in optimum dose 3.125 mg.g-1 BW and suppress SDF-1 production in high dose (31.25 mg.g-1 BW) as well as the blood glucose level measurement. This study suggest that polyherbal EMSA Eritin has a potential to decreased blood glucose level in mice model of diabetes mellitus.Keywords: CD4+, CD8+, diabetes mellitus, EMSA Eritin, SDF-1

The Effect of E. scaber Extract to TNF-α and TGF-β on Mice under Carcinogen Treatment Rizki Amalia; Sri Widyarti; Moch. Sasmito Djati
The Journal of Experimental Life Science Vol. 8 No. 2 (2018)
Publisher : Postgraduate School, Universitas Brawijaya

Carcinogen compounds are the compounds that cause DNA damage and trigger cancer. TNF-α and TGF-β are cytokines produced by immune cells and serve to maintain body homeostasis. Elephantopus scaber is a plant that prevents the cancer progression, and improve the body's immune system, so this study was conducted to determine the effect of E. scaber extract on TNF-α and TGF-β after the administration of carcinogen compounds. The study was conducted by administering the carcinogen compound DMBA with a dosage of 0.56 mg kg-1 BW and estradiol with a dose of 0.0504 mg kg-1 BW which was given alternately for 8 weeks to the Mus musculus test animals. The study was conducted in 3 groups: K- (the normal group of mice), K + (a carcinogen-induced group of mice), and P (a group of carcinogen-induced mice and E. scaber extract). The treatment was done in 1 week, 2 weeks, and 3 weeks. Immune cells were isolated from splenocytes and performed immunostaining for flow cytometry analysis. The computed relative amount is TNF-α produced by macrophages CD11b and TGF-β produced by Treg CD4CD25. The relative number of cells was analyzed by two-way ANOVA and advanced Tukey test with 95% confidence level (α = 0.05). The results showed no significant differences in the number of cytokines TNF-α and TGF-β in both the carcinogen-induced mice group and the mice group was given the extract of Elephantopus scaber for 1 week, 2 weeks and 3 weeks. Keywords: Carcinogen, Elephantopus scaber, TNF-α, TGF-β

In Vitro Immunomodulatory Activity of Aqueous Extract of Moringa oleifera Lam. Leaf to the CD4 +, CD8+ and B220+ Cells in Mus musculus Indriya Rachmawati; Muhaimin Rifa'i
The Journal of Experimental Life Science Vol. 4 No. 1 (2014)
Publisher : Postgraduate School, Universitas Brawijaya

Immune system is a system of biological structures and processes within an organism that protects against disease. It can be promoted by substance referred as immunomodulator. Usually people use synthetic drug or synthetic immunomodulator to get quick response against the disease. This habit lead to arising side effects such as nausea, bone marrow degradation, thrombocytopenic purpura and agranulocytosis. As alternative, natural immunomodulator derived from active compound in plant. The objectives of this study are to determine the effect of aqueous extract of Moringa oleifera Lam. leaf to the population of CD4+ and CD8+ T cells, and also B220+ cell on Mus musculus through in vitro study and to analyze the difference of immune response in treatment and non-treatment group (control). In this experiment we used spleen from Balb/C mice. Cells were grown in RPMI medium with three doses (10 µg/ml, 1 µg/ml, 0,1 µg/ml) of M.oleifera extract. The cells were grown for four days culture in the CO2 incubator at 37°C with 5 % CO2. The cells number and expression were analyzed by flowcytometry. Data was analyzed using one-way ANOVA with α=0,05 by SPSS 16.0 for windows with complete randomized design. The result shows that the extract has immunostimulant activity and the low dose (0,1 µg/ml) can increase the cell number of CD4+ and CD8+, while high dose (10 µg/ml) significantly increase B220+ cells compared to the control. This result strenghten that M.oleifera has immunomodulator activity to immunity system and worth to be developed into medicinal drug. Keywords: immunomodulator, in vitro, Moringa oleifera, T cell

Influence of Different Pulse Length towards Motility and Viability of Ornamental Japanese Carp (Cyprinus carpio Var. Koi) Sperm through Electroporation Method Diana Aisyah; Agoes Soeprijanto; Yuni Kilawati
The Journal of Experimental Life Science Vol. 7 No. 1 (2017)
Publisher : Postgraduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2016.007.01.06

Successful studies about implementation of electroporation method which sperm becomes gene transfer medium for fish transgenesis have reported. Motility and viability are two major factors in successful of electroporation. The objective of the study was to determine optimal pulse length of electroporation towards the motility and viability of Koi fish sperm. The study was conducted at the Central Laboratory of Biological Sciences and Reproduction of Brawijaya University. The data analysis was conducted using completely randomized design. The electroporation method was carried out using voltage of 30 volt with pulse number of 4 times. The pulse length of the electroporation (based on treatment) was 0.5, 1 and 1.5 ms. The result showed the highest percentage of motility was 3.67% in treatment A (pulse length 0.5 ms) with coefficient correlation R2 = 0.9643 and the best percentage of viability was 79.67% in treatment A (pulse length 0.5 ms) with coefficient correlation R2 = 1. Correlation between the pulse length treatment (electroporation) toward motility and viability of Koi fish sperm, in which longer duration of pulse length treatment (electroporation) would decrease the motility and viability percentage of the fish sperm.Keywords: Electroporation, Motility, Pulse length, Sperm, Viability.

Optimization of Time and Temperature Gelatin Extraction from Pink Perch (Nemipterus bathybius) Head using Response Surface Methodology (RSM) Ulfatul Mardiyah; Simon Bambang Widjanarko; Kiki Fibrianto
The Journal of Experimental Life Science Vol. 9 No. 2 (2019)
Publisher : Postgraduate School, Universitas Brawijaya

Gelatin from fisheries byproducts was very potential to be used as an alternative gelatin substitute for bovine and porcine gelatin. In this study, optimization of pink perch head gelatin extraction was carried out based on temperature and extraction time. Factorial design and Response Surface Methodology (RSM) were used to optimize the gelatin extraction process of pink perch (Nemipterus bathybius) head. The purpose of this research was to determine the optimum condition of the pink perch head extraction process based on the temperature and time of extraction. The extraction temperature (50°C - 90°C), and the extraction time (3 - 7 hours) were the independent variables. The response variables of this study were the yield of gelatin (%), gel strength (g.bloom) and viscosity (cP). The optimum extraction conditions were obtained at the extraction temperature of 74.40°C for 5.42 hours with a yield of 5.31%, the gel strength of 311.01 g.bloom and the viscosity of 5 cP. Considering its similarity to the commercial gelatin, particularly on their chemical characteristics, it can be suggested that the head of pink perch is a potential alternative of gelatin source. Keywords: Extraction, Gelatin, Optimization, Pink Perch Head

The Effectiveness of hrGFP Gene Reporter Role in Carp Fish (Cyprinus carpio) Transgenesis Process Based on Convocal Microscopy Analysis Septi Anitasari; Agus Soeprijanto; Abd. Rahem Faqih
The Journal of Experimental Life Science Vol. 5 No. 2 (2015)
Publisher : Postgraduate School, Universitas Brawijaya

Sperm cells can be used as a medium to insert foreign DNA into the egg. Electroporation humanized renilla reformis Green Fluorescent Protein (hrGFP) gene as a reporter gene is very important to know the effectiveness of gene transfer. Research is aimed to understand the effectiveness rate of hrGFP gene reporter to detect the successful gene transfer process based on the result of observation with convocal microscope over transgenic sperm level, embryo and larva of Carp Fish. Result of trans-genesis of hrGFP as sperm-mediated gene reporter must be accurately detected. Based on the result of observation with convocal microscope, it is shown that hrGFP enters the core level, indicates by PI pigmentation and thereby, hrGFP gene reporter can provide information about hrGFP fluorescence starting from sperm, embryo and larva of Carp Fish.Keywords: Carp Fish, effectiveness, gene reporter, hrGFP, sperm fluorescence.

Growth Rate and Chemical Composition of Secondary Metabolite Extracellular Polysaccharide (EPS) in Microalga Porphyridium cruentum Nurul Mutmainnah; Yenny Risjani; Asus Maizar Suryanto Hertika
The Journal of Experimental Life Science Vol. 8 No. 2 (2018)
Publisher : Postgraduate School, Universitas Brawijaya

Porphyridium cruentum microalgae have great potential to be developed as a source of active ingredients in various fields of health. It excretes secondary metabolites in the form of extracellular polysaccharides (EPS), potentially as antibacterial, antihyperglycemic, and immunostimulant components. This study aims to obtain the best culture density of P. cruentum, the active component of P. cruentum (EPS), and the chemical composition of EPS. The P. cruentum was cultivated on 15%, 20% and 25% culture stock, with an addition of silicate, Fe and vitamins in sterile seawater medium with salinity 35 ‰, pH 8, temperature 25-27°C, with continuous aeration and 2500 lux continuous 24 hours, for 14 days of culture period. The results of this study indicate differences in growth rates and abundance of microalgae to each culture stock. The 15%, 20% and 25% culture stocks each showed stationary phase at days 10th, 12th and 14th, with EPS concentrations of 10 ppt, 12 ppt, and 15 ppt, respectively. Variation of P. cruentum culture showed different EPS results, with a density of 25% capable of producing the highest EPS extract of 15,000 mg.L-1. The EPS is known to contain glucose and carboxylic acid compounds that can be utilized in the health and industrial fields. Keywords: culture, extracellular polysaccharides, Porphyridium cruentum.

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Home Page

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Contact Name Jehan Ramdani Hariyati

Contact Email jehanramdani@ub.ac.id

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Editorial Address Jl. Veteran Malang 65145

Location Kota malang, Jawa timur INDONESIA

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Contact Name
Jehan Ramdani Hariyati

Contact Email
jehanramdani@ub.ac.id

Phone
+6282333752235

Journal Mail Official
jehanramdani@ub.ac.id

Editorial Address
Jl. Veteran Malang 65145

Location
Kota malang,

Jawa timur

INDONESIA