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The Journal of Experimental Life Sciences (JELS)

jels
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Published by Universitas Brawijaya

ISSN : 20872852 EISSN : 23381655 DOI : 10.21776/ub.jels

Core Subject : Health, Science,

Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology Neuroscience

The Journal of Experimental Life Science (JELS) is a scientific journal published by Postgraduate School, University of Brawijaya as distribution media of Indonesian researcher’s results in life science to the wider community. JELS is published in every four months. JELS published scientific papers in review, short report, and articles in Life Sciences especially biology, biotechnology, nanobiology, molecular biology, botany, microbiology, genetics, neuroscience, pharmacology, toxicology, and Applied Life Science including fermentation technology, food science, immunotherapy, proteomics and other fields related to life matter. JELS is a scientific journal that published compatible qualified articles to the academic standard, scientific and all articles reviewed by the expert in their field. The Journal of Experimental Life Science (JELS) have a vision to become qualified reference media to publish the best and original research results and become the foundation of science development through invention and innovation on cellular, molecular, nanobiology, and simulation work related to life matter rapidly to the community. The Journal of Experimental Life Science (JELS) has objectives to published qualified articles on research’s results of Indonesian researchers in life science scope. JELS encompasses articles which discuss basic principles on natural phenomenon with cellular, molecular, and nanobiology approach.

Arjuna Subject : Biokimia, Genetika dan Biologi Molekuler - Biokimia, Genetika dan Biologi Molekuler (Lain-Lain)

Articles 299 Documents

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Effectiveness of Indigenous Lead (Pb) Reducing Bacteria Consortia of Waste Water Treatment in Agar Flour Industry Wasiatus Sa'diyah; Endang Suarsini; Ibrohim Ibrohim
The Journal of Experimental Life Science Vol. 6 No. 1 (2016)
Publisher : Postgraduate School, Universitas Brawijaya

Lead (Pb) is known as toxic element in environment. It is produced from processing of Agar Flour industry activities. Enhancement of Pb in the waste water of agar flour is very dangerous for human health. Effect of lead damage some organ e.g. kidney, liver, and hair. Environment standard of lead concentration on waste water based on Governor of East Java Regulation No. 72 of 2013 is 1 mg.L-1, otherwise, initial lead levels of waste water are 3.114 mg.L-1. The aim of the research is reducing the level of lead to be harmless for the environment. One method to decrease a high concentration of lead in waste water is biosorption. Consortia of Bacillus alvei and Bacillus pumilus as indigenous bacteria are used to decrease Pb level in the wastewater. The experiment was carried out with varies of wastewater concentration and bacteria 3 %, 4%, 5%, 6%, and 7%. Consortium of B. alvei dan B. pumilus at 7 % concentration have the highest potency 93.58 % to reduce lead reach 0.2 mg.L-1.Keywords: Bacteria, Consortia, Concentration, Pb, Waste of Agar Flour

Numerical Simulation of Leslie-Gower Predator-Prey Model with Stage-Structure on Predator Rima Anissa Pratiwi; Agus Suryanto; Trisilowati Trisilowati
The Journal of Experimental Life Science Vol. 8 No. 3 (2018)
Publisher : Postgraduate School, Universitas Brawijaya

In this paper, we introduce Leslie-Gower predator-prey model with a stage-structure population on the predator. This model consists of two populations, that are prey and predator populations. Here, we divide predator into two stages. Thus, we have three classes of population in this model that are prey, juvenile predator, and mature predator. The focus of this paper is to know the interaction between the population that is affected by stage-structure in predator population in the model and to study numerically the effects of stage-structure in predator population on the interaction of prey and predator. It is found that the transition rate from juvenile to mature predator is a very important parameter which may determine the long-term behavior of both prey and predator.Keywords: Leslie-Gower model, predator-prey model, stage-structure.

Effects of Low Temperature on Somatic Embryos Growth, Maturation and Planlet Regeneration of Citrus Mandarin var Batu 55 (Citrus reticulata Blanco.) Mufidatur Rosyidah; Wahyu Widoretno; Serafinah Indriyani
The Journal of Experimental Life Science Vol. 5 No. 1 (2015)
Publisher : Postgraduate School, Universitas Brawijaya

This study was aimed to determine the effect of incubation at 4°C temperature during multiplication stage of somatic embryos on growth, maturation and plantlets regeneration of citrus Mandarin var Batu 55. Globular somatic embryos were cultured on MT (Murashige & Tucker) medium and incubated at 4°C temperature for 0, 2, 4, 6, 8 weeks. Maturation of somatic embryos was induced by transfering globular somatic embryos on maturation medium (MT + 50 mgl-1 malt extract + 73 mM sorbitol + 73 mM galactose). Cotyledonary embryo was regenerated on MT medium + 50 mgl-1 malt extract + 30 gL-1 sucrose + 2 ppm GA3. The research showed that maturation of somatic embyos at 4°C temperature inhibited growth and maturation of somatic embyos. Fresh weight of somatic embryo incubated at 4°C for 2 weeks lower than fresh weight of embryo cultured without incubation at 4°C, and continuous decline in longer incubation period. Maturation percentage of embryos without incubation at 4°C temperature was 15%, but embryos incubated at 4°C temperature were lower than 9%. There was no effect of incubation at 4°C temperature during somatic embryos multiplication stage on plantlet regeneration percentage. Keywords: Citrus reticulata, maturation, plantlet regeneration, somatic embryo

Dynamic Analysis of Epidemic Model for Cholera Disease Spreading with Quarantine Tyas Husadaningsih; Wuryansari Muharini K; Aruman Imron
The Journal of Experimental Life Science Vol. 8 No. 1 (2018)
Publisher : Postgraduate School, Universitas Brawijaya

In this paper, the epidemic model of cholera disease spreading by quarantine is discussed. It is assumed that the spread of cholera not only through direct contact between susceptible human populations with bacteria but also through direct contact between susceptible human populations with infected human populations and reduced bacterial populations not only die naturally but can also be done by means of extermination bacteria. Determination of equilibrium points, existence and local stability of equilibrium points are investigated. Numerical simulations are performed to illustrate the results of the analysis.Keywords: Cholera Disease, Epidemic Model, Runge-Kutta Method 4th order, Stability, Quarantine.

The Antigenotoxic Activity of Brown Seaweed (Sargassum sp.) Extract Against Total Erythrocyte and Micronuclei of Tilapia Oreochromis niloticus Exposed by Methomyl-Base Pesticide Kilawati, Yuni; Islamy, R Adharyan
The Journal of Experimental Life Science Vol. 9 No. 3 (2019)
Publisher : Graduate School, Universitas Brawijaya

Pesticides are widely applied in the agriculture sector to protect crops and pest control. The poisonous substance of pesticides will affect all of the organisms, either target and non-target organisms. Fish can play the role of an indicator of genotoxic presence in aquatic environments. Polysaccharide extracts from sargassum have promising anti-genotoxic potential. This study aimed to analyze the anti-genotoxic activity of brown seaweed (Sargassum polycystum) methanol extract against erythrocyte and micronuclei of tilapia exposed by methomyl-base pesticide. Brown seaweed (Sargassum sp.) purchased from farmers in Sumenep Regency, Madura, East Java, then macerated using methanol 1: 3 (w / v) for 3x24 hours at room temperature. The phytochemical screening was including flavonoids, alkaloids, triterpenes, saponins, and tannins. Tilapia fish (TL ± 9-12cm) purchased from the Technical Application Unit of Freshwater Fish (UPT Perikanan Air Tawar), Sumberpasir, Malang, East Java. The result of this study showed that exposure of methomyl-based pesticides in the concentration of 4.015 ppm indicates the formation of micronuclei of 318.33 ‰. The increased concentration of extract treatment is directly proportional to the decrease of micronuclei. It means that sargassum extract can reduce the genotoxic effect on exposed tilapia by methomyl-based pesticides. The best concentration of Sargassum sp. extract that can reduce genotoxic was D (200 ppm). Keywords: Antigenotoxic, Extract, Methomyl, Pesticides, Sargassum sp., Tilapia.

Effectivity of Polyscias obtusa Simplicia as Immunomodulator on CaecaTonsil of Broiler Post Infection of Salmonella typhimurium Mutya Farsely; Muhammad Sasmito Djati; Muhaimin Rifa'i
The Journal of Experimental Life Science Vol. 3 No. 1 (2013)
Publisher : Postgraduate School, Universitas Brawijaya

Antibiotics usually used to protect the animals from pathogens, but antibiotics have residues which have negative side efect. Antibiotics can substituted with Polyscias obtusa simplicia because this plant contains a chemical compound called Flavonoid which roles in immune system as immunomodulator. This study aimed to determine the mobilization and development of CD4+, CD8+ and B220+ T-Cells lymphocytes from thymus of broiler that have been given additional feed Polyscias obtusa after infected by Salmonella typhimurium and determine the optimum dose of the addition of simplicia's Polyscias obtusa which can influence the mobilization of T-Cells lymphocyte in Thymus. The procedure are confirmation of the Salmonella typhimurium isolates, prepare the simplicia's Polyscias obtusa and Salmonella typhimurium bacterial culture, orally infections in 500 µl, prepare supplemented feeds by three doses (i.e. dose 1 (0.08%), dose 2 (0.16%) and dose 3 (0.26%)), thymus and T-Cells lymphocytes isolation and Flowcytometry analysis. The results showed that feeding with antibiotical feed + Salmonella infection and natural feed with additional Polyscias obtusa simplicia's dose 2 (0.16%) have increased the relative number of CD4+, CD8+ and B220+. T-Cells lymphocytes of thymus with different time feeding period have significant differences for relative number of CD8+. This result gave proof that Polyscias obtusa simplicia have the ability and influence to increase the body's immune system. Keywords : Broiler, Polyscias obtusa, Salmonella typhimurium, T-Cells lymphocyte, Thymus

Rosella Flower Decreases the CML Serum and Liver Inflammation of Rats Given Baked-Food Diet Silvy Amalia Falyani; Setyawati Soeharto; Edi Widjajanto; Ardhiyanti Puspita Ratna; Pia Bataif Batmomolin
The Journal of Experimental Life Science Vol. 6 No. 2 (2016)
Publisher : Postgraduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2016.006.02.03

Advanced Glycation Endproducts (AGEs) or so-called glycotoxin can be triggered by heated food in which the Maillard reaction occurs. One type of glycotoxins is CML. Accumulated N-Carboxymethyl-Lysine (CML) can cause inflammation of organs, e.g. liver. Rosella flowers contain anthocyanin compound that has anti-glycation and antioxidant effects. This study aimed to determine the effect of ethanol extract of Rosella on CML serum level, IL-6 level, and NF-ƙB activation in the liver of mice fed with baked food. This study used post-test design using 25 Wistar rats aged 3-4 months old that were divided into 5 groups, namely negative control group, positive control group, treatment groups given Rosella extract dose of 200mg.kgBW-1, 300mg.kgBW-1, and 400mg.kgBW-1. Baked feed was given for 12 weeks, and Rosella ethanol extract was administered in the 9th to 12th week. The examination on CML serum and IL-6 of the liver was using ELISA method. Immunofluorescent staining was used to determine NF-ƙB activation in the liver using a confocal microscope. CML serum is proven to increase significantly (p = 0.000). The effective dose of Rosella flower extract to prevent CML Serum increase is 200 mg.kgBW-1, whereas a dose of 400 mg.kgBW-1 can decrease IL-6 level and NF-ƙB activation. Ethanol extract of Rosella flower decrease the levels of N-carboxymethyl-lysine serum, IL-6, and NF-ƙB activation in the liver of rats given baked-food diet. Keywords: Advanced Glycation Endproducts (AGEs), Interleukin-6, N-Carboxymethyl-lysine, Rosella.

Role of Active Compounds of Bohadschia argus Inhibit Cancer Cell Survival Jantje Wiliem Souhaly; Sri Rahayu; Widodo Widodo
The Journal of Experimental Life Science Vol. 9 No. 1 (2019)
Publisher : Postgraduate School, Universitas Brawijaya

Sea cucumber is marine biota with a high economic value and also has potential for anti-cancer. The purpose of this study was to explore the mechanism of active compound of Bohadschia argus on regulating cancer cell survival. The B. argus samples were collected from the sea of Kamal Village, West Seram Maluku, then extracted by water. The constituents of water extract of B. argus were examined by LC-MS. The network among active compound and its protein target were determined by Cytoscape app. The result shows that B. argus has several active compounds, such as chondroitin sulfate, holothurin A, holothurin B, and scabraside that might play a role in cancer cell apoptosis, proliferation, and metastasis. Keywords: Active compound, Bohadschia argus, LC-MS.

Proteksi Kombinasi Minyak Wijen dan α-tocopherol Terhadap Glomerular Injury Melalui Penghambatan Stres Oksidatif Tikus Hiperkolesterolemia Dewi Sukmawati
The Journal of Experimental Life Science Vol. 3 No. 2 (2013)
Publisher : Postgraduate School, Universitas Brawijaya

Minyak wijen (MW) kaya akan polyunsaturated fatty acid (PUFA) terutama linoleat yang diketahui dapat menurunkan kadar lipid dalam darah. Namun, salah satu kelemahan PUFA sebagai hipolipidemik adalah cenderung mudah untuk teroksidasi karena struktur ikatan rangkapnya. Vitamin E (α-tocopherol) diketahui dapat melindungi struktur PUFA terhadap oksidasi serta dapat menghambat stres oksidatif. Peningkatan stres oksidatif pada hiperkolesterolemia diduga kuat berperan penting dalam patogenesa glomerular injury. Tujuan penelitian ini untuk membuktikan bahwa penambahan α-tocopherol pada minyak wijen mempunyai efek sinergis dalam mencegah glomerular injury melalui penurunan stres oksidatif. Penelitian ini menggunakan Rattus novergicus strain Wistar jantan (200 g) yang dibagi menjadi tujuh kelompok masing - masing 4 ekor, kelompok hiperkolesterol (HK), kelompok ke-2, ke-3 dan ke-4 adalah kelompok HK + MW (dosis masing-masing 0,3; 0,6, dan 1,2 ml), sedangkan kelompok ke-5, ke-6 dan ke-7 adalah kelompok HK + MW (dosis masing - masing 0.3, 0.6 dan 1.2 ml) + α-tochopherol 20 mg). Pengukuruan kadar kolesterol total, Low Density Lipoprotein (LDL), ureum dan kreatinin darah dilakukan pada minggu ke-10. Aktivitas stres oksidatif jaringan ginjal diukur dari kadar malondialdehide (MDA) menggunakan uji thiobarbituric acid (TBA) dan kadar superoxyde dismutase (SOD) jaringan dengan uji Nitroblue tetrazolium (NBT). Pemeriksaan histologis dilakukan untuk melihat deposit matriks dan jumlah sel mesangial pada glomerulus dengan pengecatan Periodic Acids Schiff (PAS). Hasil penelitian ini menunjukkan adanya perbedaan bermakna penurunan kadar MDA antara kelompok MW dengan MW + α-tocopherol pada dosis 0.3 (p = 0.000) dan 0.6ml (p = 0.001) yang menunjukkan interaksi sinergistik MW + α-tocopherol serta kecenderungan penurunan kadar SOD. Terdapat dugaan interaksi secara potensiasi dengan penambahan α-tocopherol pada minyak wijen terhadap penurunan kadar kolesterol total dan LDL. Gambar histologis glomerular injury menunjukkan bahwa kombinasi MW + α-tocopherol dapat menghambat peningkatan jumlah sel (p = 0.000) dan matriks mesangial (p = 0.614). Hasil ini menunjukkan bahwa penambahan α-tochopherol pada minyak wijen mempunyai efek yang lebih baik dibanding minyak wijen sendiri dalam menghambat glomerular injury melalui penghambatan stres oksidatif di jaringan ginjal secara sinergis. Kata kunci: α-tocopherol, glomerular injury, minyak wijen, stres oksidatif.

Effect of Banana Homogenate on Shoot Regeneration of Ciplukan (Physalis angulata L.) Varni Apensa; Retno Mastuti
The Journal of Experimental Life Science Vol. 8 No. 1 (2018)
Publisher : Postgraduate School, Universitas Brawijaya

In vitro culture techniques can be used as an alternative problem solving in an effort to provide Ciplukan plant as a raw material of herbal medicine in a sustainable manner. The purpose of this study was to observe the effect of banana homogenate (BH) added into culture medium on shoot regeneration of Ciplukan (Physalis angulata L.) and plantlet development until acclimatization stage. The experimental design used was a randomized factorial group. The first factor was the two varieties of banana fruit namely, Ambon and Raja. The second factor was concentration of BH with four levels, 0 (control), 2.5%, 5% and 7.5 % and each treatment combination was repeated five times. Explant used in this research was the third node of 2 weeks old in vitro shoot after subculture to MS0 medium. Data analysis used ANOVA test. Eight week after culture (WAC) medium containing BH induced higher number of shoot compare to medium without BH. The addition of 5% BH var. Ambon had significant effect on increasing shoot number. Well rooted plantlets produced in MS0 rooting medium also showed high survival rate (66.7%< - 100%) two weeks after transferred to greenhouse. Therefore, the addition of BH into in vitro medium can be an alternative method for micropropagation of Ciplukan.Keywords: Banana homogenate, Ciplukan, micropropagation, nodal explants.

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Home Page

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Contact Name Jehan Ramdani Hariyati

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Editorial Address Jl. Veteran Malang 65145

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Contact Name
Jehan Ramdani Hariyati

Contact Email
jehanramdani@ub.ac.id

Phone
+6282333752235

Journal Mail Official
jehanramdani@ub.ac.id

Editorial Address
Jl. Veteran Malang 65145

Location
Kota malang,

Jawa timur

INDONESIA