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The Journal of Experimental Life Sciences (JELS)
Published by Universitas Brawijaya
ISSN : 20872852     EISSN : 23381655     DOI : 10.21776/ub.jels
Core Subject : Health, Science,
Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology Neuroscience
The Journal of Experimental Life Science (JELS) is a scientific journal published by Postgraduate School, University of Brawijaya as distribution media of Indonesian researcher’s results in life science to the wider community. JELS is published in every four months. JELS published scientific papers in review, short report, and articles in Life Sciences especially biology, biotechnology, nanobiology, molecular biology, botany, microbiology, genetics, neuroscience, pharmacology, toxicology, and Applied Life Science including fermentation technology, food science, immunotherapy, proteomics and other fields related to life matter. JELS is a scientific journal that published compatible qualified articles to the academic standard, scientific and all articles reviewed by the expert in their field. The Journal of Experimental Life Science (JELS) have a vision to become qualified reference media to publish the best and original research results and become the foundation of science development through invention and innovation on cellular, molecular, nanobiology, and simulation work related to life matter rapidly to the community. The Journal of Experimental Life Science (JELS) has objectives to published qualified articles on research’s results of Indonesian researchers in life science scope. JELS encompasses articles which discuss basic principles on natural phenomenon with cellular, molecular, and nanobiology approach.
Arjuna Subject : Biokimia, Genetika dan Biologi Molekuler - Biokimia, Genetika dan Biologi Molekuler (Lain-Lain)
Articles 299 Documents
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Kajian Penggunaan Ciprofloxacin terhadap Histologi Insang dan Hati Ikan Botia (Botia macracanthus, Bleeker) yang Diinfeksi Bakteri Aeromonas hydrophila Sukarni Sukarni; Maftuch Maftuch; Happy Nursyam
The Journal of Experimental Life Science Vol. 2 No. 1 (2012)
Publisher : Postgraduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1118.574 KB) | DOI: 10.21776/ub.jels.2012.002.01.02

Abstract

Aeromonas hydrophila mampu menyebabkan terjadinya infeksi dan kematian ikan botia dalam waktu yang relatif singkat pada konsentrasi yang tinggi, terbukti dengan konsentrasi 108 selml-1 menyebabkan 50% ikan uji mati. Hasil pengamatan histopatologi pada ikan terinfeksi menunjukkan ginjal yang mengalami degenerasi hyaline, munculnyavakuola yang disebabkan lisis pada glomerulus dan kemudian hancur, nekrosis pada glomerulus, terdapat banyak koloni bakteri A.hydrophila didalam ginjal, serta terjadi infiltrasi limfosit. Sel hati tidak terlihat jelas karena tertutup infiltrasi limfosit dan koloni bakteri A.hydrophila yang sangat banyak, nekrosis yang menyebabkan vakuola, terdapat infeksi sekunder bakteri yang belum teridentifikasi, serta terjadi cloudy swelling. Pada bagian insang, nekrosis lamela primer menyebabkan munculnya vakuola, kongesti pada lamela primer dan edema pada lamela sekunder, infiltrasi limfosit, poliferasi sel/fusi lamela serta hyperplasia pada lamela primer. Sementara itu pada ikan botia yang terinfeksi A.hydrophila tetapi kemudian diobati, ginjal mengalami perbaikan glomerulus dan kapsula bowman sehingga terlihat jelas, tubuli berbentuk seperti donat dengan corak titik dan garis, tidak ada lagi nekrosis dan vakuola, tidak ditemukan lagi koloni bakteri, terjadi perbaikan hyaline yang mengalami degenerasi, jaringan hematopoietic (pembentuk sel-seldarah merah) terlihat jelas dengan inti yang bulat. Pada bagian hati, tidak ditemukan lagi koloni bakteri A.hydrophila dan bakteri sekunder lainnya sehingga hepatosit terlihat jelas dengan bentuk polyhedral dengan inti 1-2, jumlah eritrosit terlihat normal, karena tidak ada lagi infiltrasi limfosit. Akan tetapi masih terdapat penyumbatan pada vena centralis yang dipenuhi oleh eritrosit. Pada insang tidak ada lagi nekrosis dan vakuola pada lamela primer sehingga jaringan terlihat solid, namun ditemukan infeksi sekunder parasit Monogenea sp. Tidak ada lagi infiltrasi limfosit, lapisan epithelium terlihat 1-2 lapis, tidak ada lagi edema dan fusi lamela sehingga lamela tampak jelas dengan ukuran panjang yang bervariasi. Kata Kunci : histopatologi, Aeromonas hydrophila, Ikan Botia
The Quality of Ornamental Japanese Carp (Cyprinus carpio var. Koi) After Electroporation as a Gene Material Transfer Method Saidah Luthfiyah; Agoes Soeprijanto; Yuni Kilawati
The Journal of Experimental Life Science Vol. 6 No. 2 (2016)
Publisher : Postgraduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1018.176 KB) | DOI: 10.21776/ub.jels.2016.006.02.07

Abstract

Spermatozoa are male gametes that have an advantage in acting as gene transfer media (SMGT), because in transferring genetic material, sperm uses relatively natural vectors. This study was conducted to know sperm quality of Koi after short electroporation with different voltages using Gene Pulser. The study was conducted using a complete randomized design (RAL) with three (3) replications and five (5) treatments, namely: 10 V.cm-1, 20 V.cm-1, 30 V.cm-1, 40 V.cm-1, and 1 control. Electroporation was done with square wave, 1 ms duration of electroporation, 4 times of electroporation shocks with 0.1 s duration. The results showed that electroporation with different voltage levels significantly affected the motility and viability of sperm. Based on orthogonal polynomial analysis, it was known that the treatment affected motility and viability of sperm with a linear equation y = -0.9x + 5, 4 R² = 0.920 and r = 0.96 for motility and y = -11.26x + 91.61 R² = 0.823 and r = 0.91 for viability, meaning that the higher the level of electroporation the lower the viability and sperm motility produced. From the results of this study, it can be suggested that the electroporation of Koi sperm as a gene transfer medium should be performed at a voltage of 10 V.cm-1. Keywords: electroporation, Koi, sperm quality, transgenesis.
Dietary Rice Bran Plays A Significant Role in the Hepatoprotective Effect in Hypercholesterolemic Rats Yulianti Antula; Dolly Irnawati Neno; Arie Srihardyastutie; Chanif Mahdi
The Journal of Experimental Life Science Vol. 9 No. 2 (2019)
Publisher : Postgraduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1016.513 KB) | DOI: 10.21776/ub.jels.2019.009.02.08

Abstract

Cholesterol is obtained through biosynthesis and diet. When a level of cholesterol is above the normal level, this condition caused hypercholesterolemia. Long-term administration of synthetic chemical drugs can cause liver damage. Therefore, alternative natural medication is needed. One of the alternatives that can be used is the rice bran (RB), which contains antioxidant and crude fiber. This study is aimed at finding out the potential utilization of RB on total cholesterol level, liver enzyme as aspartate aminotransferase (AST) and alanine aminotransferase (ALT), malondialdehyde (MDA), and the changes in liver tissue. This study uses five groups of rats: the negative control group, positive control group, and three therapy groups with the dosage of 270 mg kg-1, 540 mg kg-1, and 810 mg kg-1 of body weight. This study shows that therapy using RB can significantly decrease the cholesterol level, AST, ALT, and MDA (p<0.01). The total cholesterol level is 21%, AST and ALT activities can be reduced to 54% and 64%, the level of MDA reduced to 79% and can repair the liver tissue. This study shows that RB can be effectively used as hepatoprotective in rats with hypercholesterolemia. Keywords: AST and ALT, Hypercholesterolemia, Rice Bran, Total Cholesterol Level.
Bone Marrow Cells Lymphocyte Activity of Pregnant Mice with Therapy of E. scaber and S. androgynus Post Infection Salmonella typhimurium Muhammad Basyaruddin; Mochammad Sasmito Djati; Sri Rahayu
The Journal of Experimental Life Science Vol. 5 No. 1 (2015)
Publisher : Postgraduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1112.49 KB) | DOI: 10.21776/ub.jels.2015.005.01.09

Abstract

Pregnancy can cause immune system changes. It is characterized by a decrease in the activity of immunocompetent cells. The use of antibiotics was intended to combat pathogenic microorganisms, but antibiotics have negative effects on pregnant women. The use of antibiotics can be replaced with extracts of Elephantopus scaber and Sauropus androgynus because both plants have chemical compounds that act as immunomodulators. This study was aimed to determine the activity of lymphocytes B220+, TER119+, and GR-1+ on bone marrow pregnant mice given the combination of extracts of Elephantopus scaber and Sauropus androgynus after infected with Salmonella typhimurium. This research uses seven treatment groups name: (K-) 0.05% NaCMC without bacterial infection; (K+) 0.05% NaCMC infected by bacteria; (P1) E. scaber 200 mg.kg-1BW infected by bacteria; (P2) E. scaber 150 mg.kg-1BW and S. androgynus 37.5 mg.kg-1BW infected by bacteria; (P3) E. scaber 100 mg.kg-1BW and S. androgynus 75 mg.kg-1BW infected by bacteria; (P4) E. scaber 50 mg.kg-1BW and S. androgynus 112.5 mg.kg-1BW infected by bacteria; and (P5) S. androgynus 150 mg.kg-1BW infected bacteria. The initial dose of E. scaber and S. androgynus was 50 mg.kg-1. Each treatment has three repetitions, surgery performed on day 12th and 18th. Lymphocyte cells isolated from bone marrow, the obtained results were analyzed by flowcytometry and statistical analysis using SPSS 16.0 one-way ANOVA, Tukey test and path. Based on the results from ANOVA tables, the formulations on mice that can restore their normal conditions with B220+ cells is E. scaber 150 mg.kg-1BW and S. androgynus 37.5 mg.kg-1BW, TER119+ cells is S. androgynus 150 mg.kg-1BW, while the GR-1+ cells affected by the surgery. Those three dose formulations can be used to obtain the optimum value which can increase the number of lymphocytes and not harmful to the developing fetus. Keywords: Bone marrow, Elephantopus scaber, Lymphocyte, Pregnant mice, Sauropus androgynus
Patellar Giant-Cell Tumor: a Case Report Istan Irmansyah Irsan; Satria Pandu Persada; Rakhmad Aditya
The Journal of Experimental Life Science Vol. 8 No. 2 (2018)
Publisher : Postgraduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (968.597 KB) | DOI: 10.21776/ub.jels.2018.008.02.08

Abstract

Giant cell tumor (GCT) found mostly in the long bones metaphysis or epiphysis. GCT usually occur between the ages of a third and fourth decade and locally aggressive. Giant-Cell Tumor seldom affects the patella. Multicentric forms rarely reported. A fourteen-year-old female with a lump at her left patella since one-year associated with slight pain that aggravated by activity for six months. Plain X-ray left knee AP, and lateral views reveal expansile lytic lesion in left patella with thinning of the anteroinferior cortex and sclerotic septa within. MRI of left knee shows approximately 3x2x2 cm heterogeneous lobulated expansile soft tissue mass in left patella extending up to the patellofemoral joint with fluid-fluid appearance. From fine needle aspiration cytology, resulting giant-cell tumor with the differential diagnosis of an aneurysmal bone cyst. The operation already performed. Curettage, bone graft, and a biopsy taken. An immunocytochemical smear was performed and confirmed as a patellar giant-cell tumor. Six months after excision of the tumor, the patient complained no arthralgia and have a full range of motion for the knee. Keywords: Giant-cell tumor, knee lump, knee pain, patella 
Biosistem Pertanian Apel Lokal Malang Dian Siswanto; Irfan Mustafa; Gustini Ekowati; Muhamad Imam; Endri Purnomo
The Journal of Experimental Life Science Vol. 1 No. 2 (2011)
Publisher : Postgraduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2011.001.02.06

Abstract

Biosistem yang digambarkan pada hasil observasi di sini adalah suatu keadaan faktor biotik dan abiotik pada pertanian apel yang tidak terbatas pada suatu nilai yang tetap. Data  yang diperoleh dari tiga tahapan penelitian ini diharapkan dapat dipergunakan sebagai informasi yang mendukung upaya konsevasi pertanian apel. Tahapan penelitian tersebut meliputi evaluasi nutrisi makro tanah dan tanaman, formulasi pengomposan kotoran sapi diperkaya (menggunakan bakteri indigenus) dan eksplorasi tanaman penutup tanah pertanian apel. Kandungan beberapa nutrisi makro untuk tanah pertanian apel Bumiaji menunjukkan nilai rata-rata yang berada pada kisaran sedang dan tinggi. Hasil analisis makro nutrisi daun apel yang kemudian dibandingkan dengan standar menurut Utah Fertilizer Guide menunjukkan bahwa makro nutrisi N, P, Ca berada pada status normal, K berada pada status kurang dan Mg berlebih. Ketersediaan nutrisi di tanah dapat dipelihara dengan penambahan kompos. Kotoran sapi yang diperkaya dengan bakteri indigen mampu mendekomposisi kotoran sapi dalam waktu 2-3 minggu. Proses dekomposisi kotoran sapi berlangsung lebih cepat pada pemberian bakteri indigenus yang mempunyai kemampuan selulolitik dan sekaligus proteolitik serta amilolitik apabila dibandingkan kontrol yang tanpa pemberian inokulum bakteri. Pada pertanian apel di Poncokusumo, kondisi lahan dengan sistem penyiangan cs (cukup sering) dan sj (sangat jarang) tampak lebih stabil daripada ss (sering sekali) berdasarkan nilai ID (indek diversitas), DR (dominansi relatif) dan INP (indek nilai penting). Hal ini diperjelas oleh nilai biomassa tanaman. Biomassa yang lebih tinggi menyebabkan suhu tanah yang lebih rendah karena jumlah TPT yang banyak mempunyai konsekuensi mengandung banyak air pada jaringannya. Kata kunci: Apel, nutrisi makro, kompos diperkaya, tanaman penutup tanah
The Role of Local Hydromacrophytes in Leachate Phytoremediation Performed Using Constructed Wetland System Sophia Laily; Bagyo Yanuwiadi; Catur Retnaningdyah
The Journal of Experimental Life Science Vol. 7 No. 1 (2017)
Publisher : Postgraduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1252.487 KB) | DOI: 10.21776/ub.jels.2016.007.01.07

Abstract

The purpose of this research is to analyze the effectiveness of using local hydromacrophytes for performing leachate phytoremediation in constructed wetland (CW) system. It was an ex situ experiment carried out in a glass house by means of free water surface (FWS) CW reactors containing sand and gravel substrates in 3:5 ratio. The reactor was waterlogged by 9 L leachate. The phytoremediation tests were performed in two-factor treatment design involving hydromacrophyte species and hydraulic retention time. Species of local hydromacrophyte used in this research included Alternanthera sessilis, Commelina nudiflora, Paspalum conjugatum, Typha angustifolia and the polyculture of the four species. The improvements in leachate qualities were identified through decreases of physico-chemical parameters. Among the mean values of the percentages indicating the decreases of physico-chemical leachate parameters, the greatest ones were resulted from the treatments using T. angustifolia and A. sessilis on the 30th day. It has proven in the experiment that these two species of hydromacrophyte perform the most effective process of improving leachate quality.Keywords: constructed wetland, leachate, local hydromacrophytes, phytoremediation.
Phytochemicals and The Ability of Plantago major Linn. Extract to Inhibit The growth of Aeromonas hydrophila Annisa Farhana Dewi; Arief Prajitno; Ating Yuniarti
The Journal of Experimental Life Science Vol. 9 No. 2 (2019)
Publisher : Postgraduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1120.058 KB) | DOI: 10.21776/ub.jels.2019.009.02.02

Abstract

Aeromonas hydrophila is a type of gram-negative bacteria that can harm humans and major animals that have poikilotherm properties such as fish and shrimp. These bacteria can cause haemorrhages in fish until death and causing fish farming production to failure. The use of medicinal plants has been trusted by people all over the world to overcome various disease problems, one of which is a disease caused by bacteria. Plantago major L. is known that able to inhibit the growth of both gram-positive and gram-negative bacteria. The purposes of this study were to identify the extracted compounds contained in Plantago major L., using FTIR and UV-VIS spectrophotometer and to evaluate antibacterial activity against A. hydrophila. The results of this study showed that crude extracts of Plantago major L. contained polar compounds such as phenols, flavonoids, saponins, and tannins. The presence of phenols and flavonoids was confirmed by the results of FTIR and UV-VIS. Furthermore, the crude extract of Plantago major L. significantly inhibited the growth of A. hydrophila (P <0.05).Keywords: Antibacterial, Co-culture, Extraction, FTIR, UV-VIS.
Antibacterial Activity of Some Herbs Water Extract against Escherichia coli Bondan Prakoso; Eko Widodo; Edhy Sudjarwo
The Journal of Experimental Life Science Vol. 5 No. 2 (2015)
Publisher : Postgraduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (911.084 KB) | DOI: 10.21776/ub.jels.2015.005.02.06

Abstract

The research aims to select the highest diameter inhibition zone of several types of herb water extract against Escherichia coli and find the best herb water extract towards minimum inhibition concentration. It was divided into two parts, in the first stage was to evaluate antibacterial activity of herbs water extract and the second stage was to evaluate concentration of extract to effectively inhibit growth of E. coli. In the first experiment there were 6 treatments; P0 (aquadest 100%), P1 (antibiotic tetrachlor), P2 (Zingiber officinale extract), P3 (Z. officinale var. Rubrum extract), P4 (Kaempferia galanga extract) and P5 (Z. zerumbet L. extract) tested to find the best herbs on the basis of diameter inhibition zone. In the second experiment 6 treatments were used namely from 0%, 50%, 60%, 70%, 80%, 90% and 100% of Z. officinale var. Rubrum extract tested for Minimum Inhibition Concentration (MIC) against E. coli. The results showed that among the herbs, Z. officinale var. Rubrum extract had the strongest antibacterial activity based on diameter of inhibition zone against E. coli. While as low as 50% of Z. officinale var. Rubrum extract showed an MIC although the highest activity indicated when 100% of Z. officinale var. Rubrum extract used.Keywords: Z. officinale var. Rubrum, diameter of inhibition zone, MIC and E. coli.
The Alteration Tissue Structure of Digestive diverticulum of Anodonta woodiana Induced by Lead Renanda Baghaz Dzulhamdhani Surya Putra; Sri Andayani; Hartati Kartika Ningsih; Diana Arfiati; Asus Maizar Suryanto Hertika
The Journal of Experimental Life Science Vol. 8 No. 2 (2018)
Publisher : Postgraduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1617.255 KB) | DOI: 10.21776/ub.jels.2018.008.02.03

Abstract

Accumulated the toxic compound such as lead in A. woodiana caused an alteration in tissue. The aim of the present study is observation through histology to determine the alteration of the structure of digestive diverticulum tissue. A. woodiana were maintained in water tub that treated lead II nitrate exposure (Pb(NO3)2) 0, 15, 25, 35 mgL-1 for 7 days to determine the alteration of the structure of damaged the digestive diverticulum and determining water quality such temperature, DO and pH. The highest damage occurs in digestive diverticulum treated 35 mgL-1  due to the many alteration tissue structure such as hyperplasia, edema, atrophy, necrosis with the percentage of total damage of about 35%. the lowest alteration tissue structure occurs in 15 mgL-1 treatment group of the digestive diverticulum. the temperature was observed at about 25.3 - 26.4°C, pH about 6-7, and DO about 6.1-6.7 mgL-1. Thus, the increment of lead dose treatment induces increment of tissue structure alteration in digestive diverticula tissue, therefore, might promote the death of Anodonta woodiana. Keywords: Anodonta woodiana, digestive diverticulum, histology

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