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Journal of the Medical Sciences (Berkala Ilmu Kedokteran)
Published by Universitas Gadjah Mada
ISSN : 01261312     EISSN : 23563931     DOI : http://dx.doi.org/10.19106/JMedSci005303202101
Core Subject : Science,
Immunology & microbiology Neuroscience
Journal of the Medical Sciences (JMedSci) or Berkala Ilmu Kedokteran (BIK) is an international, open-access, and double-blind peer-reviewed journal, published by Faculty of Medicine, Public Health and Nursing, Universitas Gadjah Mada Yogyakarta Indonesia. JMedSci aiming to communicate high-quality articles in the areas of biomedical science from basic to clinical sciences.The journal welcomes papers from original articles, case reports, reviews, and book reviews. All papers published in JMedSci are freely available as downloadable pdf files. The journal began its publication on March 1973 and published quarterly (January, April, July, and October). JMedSci is abstracted and indexed in DOAJ, Crossref, Google Scholar, Sinta, Indonesia One Search. JMedSci is accredited by Directorate of General Higher Education, the Ministry of Research, Technology, and Higher Education, Indonesia
Arjuna Subject : Kedokteran - Kedokteran (Lain-Lain)
Articles 2,170 Documents
 101   102   103   104   105 
Serological examination using lipoarabinomannan antigen for the diagnosis of pulmonary tuberculosis Ning Rintiswati Ning Rintiswati
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 29, No 03 (1997)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

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Abstract

Tuberculosis is still an important public health problem, especially in the developing countries. Efforts have been made to overcome the problem. However, tuberculosis control program has not yet been satisfactory. This is due to various factors which might affect the development of the disease, including poverty, lack of understanding about the disease, relatively long period of time needed for treatment as well as increasing number of resistant strain against antimycobacterial drugs. One of the most important ways in controlling tuberculosis is case finding. An acurate and reliable diagnostic kit is also needed to establish the diagnosis. In this study we evaluated sensitivity and specificity of lipoarabinomannan as antigen for serological examination (Mycodot) in patients suspected of having tuberculosis. Using culture of sputum as gold standard, we observed that the sensitivity and specificity of Mycodot were 50% and 70.59%, respectively. This sensitivity was much lower than that of previous study. Interestingly, we found that 47,55% of suspected cases were negative for all three different examinations (acid fast, culture and Mycodot). With respect to this finding it is suggested that better and proper microbiological examination is required to diagnose patient with suspected tuberculosisKey words: Tuberculosis - Lipoarabinomannan - Mycodot - Acid fast - Mycobacterial Culture
Serum iron level shortly after iron supplementation shortly after and 2 hours after meal in women with iron deficiency anemia ErnaKristin, Muhammad Hakimi Sri Kadarsih Soejono,,Lukman Hakim
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 41, No 03 (2009)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

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Abstract

Background: Incidence of anemia in women in developing countries is still high, that is, around 43%. This incidence rate is far more hig'her that that in industrial countries, which is ranged between 10-12 %. The prevalence of iron deficiency anemia is still high, particularly in developing countries. The cause of the high prevalence of iron deficiency anemia is not known, since it involves various factors. Two of the probable etiologic factors is variability in dosage administration, and the effect of co-administered food. Studies on the pharmacokinetic 'of iron after single dose iron tablet administration in women with anemia and pharmacokinetic of iron coadministered with food in healthy women have been done, but study on repeated dose has never been conducted. Objective: To understand serum iron level after ingestion of repeated dose of iron shortly after and 2 hours after meal for 12 weeks in women with iron deficiency anemia. Method: The research design was a fase II clinical trial. Subjects were 24 women with iron deficiency anemia, classified into two groups, who were treated as follows: the first group was consisted of 12 women with iron deficiency anemia, treated with twice-a-day ferrous sulphate tablet @ 300 mg orally, given shortly after meal for 12 weeks; the second group was consisted of 12 women with iron deficiency anemia, treated with twice-a-day ferrous sulphate tablet @ 300 mg orally, given 2 hours after meal for 12 weeks. Blood samples were taken in week 2,4, 6, 8, 10, and 12 after treatment. Serum (ferric) iron level was measured with Vitros Fe Slides method. Result: Minimum, maximum, and average steady-state iron levels (Css min, Css max, Css average) of treatment 1 were 108,78:t 13.79 ug/dL, 121 .44:t 15.79 ug/dL, and 115.11 :t 13.13 ug/dL (mean:t SEM), respectively; while minimum, maximum, and average steady-state iron levels (Css min, Css max, Css average) of treatment 2 were 115.15 :t 46.27 ug/dL, 141.36:t 61.36 ug/dL, and 124.92:t 53.43 ug/dL (mean:t SEM), respectively. No statistical significant difference were found within treatment in minimum steady-state level between week 2, 4, 6, 8, 10, 12 after treatment. There was also no significant difference in minimum steady-state level between treatment group in week 2, 4, 6, 8, 10, and 12. , Conclusion: There were no differences inserum iron level after ingestion of repeated dose of iron shortly after and 2 hours after meal for 12 weeks in women with iron deficiency anemia. Key Words: iron supplementation-serum iron-iron deficiency anemia-steady-state iron level
Thymoma Laporan Kasus Dengan Tinjauan Pustaka Roekmini Soedibjo Roekmini Soedibjo
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 17, No 02 (1985)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

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Abstract

Three cases of thymoma were reported. Each was located in the anterior part of the media - stinum. The symptoms were dyspnea, cough, and increased blood pressure which possibly is caused by the pressure on the mediastinal organs by the tumor. In all cases either the macroscopic-or the microscopic examinations were benign. Histologically the tumor showed areas of epithelium cells and lymphocytes separated by connective tissue's septum which were seen also in the gross picture. No invasive tumor was found in the surrounding tissues or organs. There was no myasthenia gravis found in all the three cases. Key Words: mediastinal pressure's symptom - thymoma - tumor - thymus - lymphoma
Impek sosial dan resosialisasi penderita kejang R. Soejono Prawirohardjo R. Soejono Prawirohardjo
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 10, No 02 (1978)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

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Abstract

Keyword : penderita kejang
Retkulocyte production index in various anemia Setyawati Setyawati
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 37, No 01 (2005)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

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Abstract

Background: Reticulocyte production index (IPR) is used to determine the classification of functional anemia. More specific laboratory parameters are used in managing patient. There were some inappropriate of IPR correlation with the diagnosis of anemia in clinical practice.Objectives: The study was designed to know normal hematocrit in Sardjito General Hospital, to determine IPR in various anemia and to compare with standard IPR and final diagnosis in anemia. Methods: Observational study was done in patients with anemia who whole blood examination in uderwent Clinical Pathology Laboratory on January 2001 until May 2002. Reticulocyte and hematocrit examination were performed before treatment. Patients were followed until. The diagnosis of anemia was established based on the clinical manifestations and laboratory results. Normal mean calculated hematocrit was based on age and sex. IPR was calculated based on standard hematocrit (45%) and mean normal hema-tocrit.Results: The normal mean hematocrit in male, female, children and infant were 43%, 39%, 40% and 48%, respectively. There was significant difference between the 2 formulas. The concordance of IPR determination in hipoproliferative anemia and maturation defects were 100%, in hemolytic/hemorrhagic anemia and maturation defect was 23,5%. The IPR of hypoproliferative anemia and maturation defect anemia differed significantly with haemolytic/ hemorrhagic anemia. IPR determination using factual hematpcrit show a better correlation with classification of functional than using standard hematocrit of 45%.Conclusion: IPR determination based on normal calculated hematocrit mean gave more benefit in increasing the correlation with classification of functional anemia. IPR value showed inappropriate result in hemolytic/hemorhagic anemia probably due to comorbidities.Key words: IPR - functional clasification anemial - anemia - normal calculated hematocrit
The effect of bee propolis against Plasmodium berghel Infection in Swiss mice Mahardika AW Mahardika AW
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 35, No 2 (2003)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

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Abstract

Background: Malaria is one of the most important parasitic disease and still as one of the world's public health problems among another tropical diseases.. One of the difficulty on controlling malaria is the resistance of malaria parasite to antimalaria drugs, therefore it is needed further researches to find alternative drugs of antimalaria. Propolis, a beehive product widely used in this folk medicine, has attracted the attention of researchers to elucidate its biological properties. Its anti-microbial properties are not confined to bacteria. Both in vitro and in vivo tests have shown propolis to be excellent in inhibiting the growth of many protozoa such as Trichomonas vaginalis and Giardia lamb/ia.Objective: This study was undertaken to know the propolis effect on Plasmodium berghei infection in Swiss miceMethods: Swiss mice were divided into 8 groups of 20 mice. Four groups were given 3.9 mg propolis orally every 2 days for two weeks before infection while the other four groups were not. Infection with P. berghei 1x107 was given intraperitoneally. Each four groups were treated after infection as follows : propolis , combination of propolis and chloroquine, chloroquine and none as negative control. Treatment with chloroquine injection was given within 3 days started at day-1 after infection with dose of 1.56, 1.56, and 0.8 mg. Treatment with propolis was given within 3 days also at dose of 3.9 mg/day. The effect of propolis was evaluated according to the percentage parasitemia counted every day started at day-1 after infection and the pathologic effect on the liver, spleen kidney and brain.Result: Propolis could not overcome the infection but inhibit the growth of Plasmodium in the erytrocytes and prolong life span of the mice. Pathologic affect propolis on the liver is mild inflammation while on the spleen, kidney and brain seems to inhibit organ damage.Conclusion: Those result showed the effect of propolis as an Immunomodulator.Key words: malaria - propolis - parasitemia - pathologic effect - immunomodulator.
Management of myocardial infarction and the post-infarction patient Ahmad H. Asdie Ahmad H. Asdie
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 24, No 02 (1992)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

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Abstract

Acute myocardial infarction is the most common form of premature death in the industrial world. Its early recognition and active intervention are becoming important in the light of recent advances, particularly the use of streptokinase shortly after the onset of the infarct. Cardiopulmonary resuscitation (CPR) is important both inside and outside the hospital. General practitioners should be confident in performing both basic life support and advanced cardiac life support since the potential needs for resuscitation is becoming great.In semi-rural areas where the general practitioner is usually the first to be called for assistance, the use of advanced resuscitation and life support does save lives. The defibrillator is an essential piece of equipment for monitoring, diagnosis, management and treatment in these cases.Following myocardial infarction the family physician has a major role to play in rehabilitation and tertiary prevention by prescribing beta-blockers, starting within two weeks of the infarct.Key Words: myocardial infarction - premature death - CPR - streptokinase - defibrillator
Model terapi gen menggunakan sel fibroblas rekombinan yang dibungkus mikrokapsul. Suatu Kajian In Vivo M. Mansyur Romi M. Mansyur Romi
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 28, No 03 (1996)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

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Abstract

Current approaches to human gene therapy focus on insertion of a desired gene into autologous cells such as fibroblasts. hepatocytes, bone marrow stem cells or lymphocytes. An alternate strategy to gene therapy through genetic modification of the patient's own cells is to implant into different recipients the same engineered cell line under immunologically isolated conditions. This in vivo study shows that human growth hormone (hGH) as a novel reporter gene product from microencapsulated genetically modified mouse cells is detectable in the circulation of allogenic recipient mice. On day 3 of post implantation no hGH was detected, 3 ng/ml of hGH was detected on day 12 and increased during the next 9 days up to 10-fold. The high level of hGH was maintained during days 21 - 33 post implantation and went down afterward to the baseline on day 45. No hGH was detected in the control mouse implanted with the same amount of free recombinant cells.Key Words: Gene therapy - autologous cells - genetic modification - recombinant cells - lymphocytes
The effect of mahkota dewa (Phaleria macrocarpa (Scheff) Boerl) leaf etanolic extract on splenic NK 1.1 cells activity Muhammad Ghufron Marsetyawan Soesatyo Sofia Mubarika Haryana , Sismindari
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 40, No 03 (2008)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

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Abstract

Background : Mahkota dewa (Pheleria macrocarpa (Scheff, Boerl) is an herbal medicine which has been used since many years as traditional medicine in Indonesia against infections.Objective: The study was aimed to know the effect of mahkota dewa extract on cellular immune response, in particular NK1.1 cell activities.Methods: Forty C57BL/6 mice used in this study were divided into 8 groups. In this study, different doses of etanolic extract of mahkota dewa leaf were used i.e. 1.05 mg, 2.1 mg, and 4.2 mg/20g BW. Groups I, II, and III were treated with 1 mg, 2.1 mg, and 4.2 mg/20 g BW of the extract daily for 30 days. In group IV, V, and VI the extract was given simultaneously during 30 days, then the mice were infected with 104 cfu of Listeria monocytogenes (A TCC-191151 for stimulating their immune responses. Whereas group VII was untreated control group, and group VIII received only Listeria monocytogenes. All mice were then sacrificed 48 hours after the last treatment. Splenocyte NK1.1 cells were collected then cultured with YAC-1 (ATCC:TIB 1601 target cells for killing activity assay, expressing NKG2D, CD122 and IFNy assay. Results: The results showed that an oral administration of the extract significantly increased the killing activity of splenic NK 1.1 cell against the target, Y AC-1 cell. Moreover, the extract promoted the secretion of IFN-y from NK1.1 cells, and also induced expression of both surface molecule NKG2D and CD 122. The strongest effect stimulation was on the dosis of 2.1 mg/20 g BW.Conclusion: The extract had effect to augment splenic NK1.1 cell activities, as indicated by increasing their killing activity, expression of surface molecules and IFN-y production.Key words: pha/eria macrocarpa - splenic NK1.1 cell activity - YAC-1 cell - IFNy - NKG2D and CD122 surface molecules
Pengaruh Sistemik Obat Tetes Mata. Pengaruh Tetes Mata Maleat Timolol Terhadap Tekanan Darah dan Detak Nadi Tanpa Beban: Penelidan Pendahuluan Mu'tasimbillah Ghozi Mu'tasimbillah Ghozi
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 21, No 03 (1989)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

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Abstract

Timolol maleat, a new beta-adrenergic blocking agent, lowers intraocular pressure by decreasing actuous productiOri. It available in 0.25%;and 0.50% solution as an eye drop. The systemic effect of the timolol maleat 0.26% eye drops were observed among healthy medical students: The pulse rate and systolic blood pressure were insignificantly decreased but diastolic blood pressure was, insignificantly increased.Key Words: timolol maleat -- beta-adrenergic blocking agents -- blood pressure -- pulse rate -- intraocular pressure

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