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Journal of the Medical Sciences (Berkala Ilmu Kedokteran)
Core Subject : Science,
Journal of the Medical Sciences (JMedSci) or Berkala Ilmu Kedokteran (BIK) is an international, open-access, and double-blind peer-reviewed journal, published by Faculty of Medicine, Public Health and Nursing, Universitas Gadjah Mada Yogyakarta Indonesia. JMedSci aiming to communicate high-quality articles in the areas of biomedical science from basic to clinical sciences.The journal welcomes papers from original articles, case reports, reviews, and book reviews. All papers published in JMedSci are freely available as downloadable pdf files. The journal began its publication on March 1973 and published quarterly (January, April, July, and October). JMedSci is abstracted and indexed in DOAJ, Crossref, Google Scholar, Sinta, Indonesia One Search. JMedSci is accredited by Directorate of General Higher Education, the Ministry of Research, Technology, and Higher Education, Indonesia
Articles 2,170 Documents
Pendidikan Kedokteran dan Pelayanan Kesehatan T. Jacob T. Jacob
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 8, No 04 (1976)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

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Abstract

Pendidikan kedokteran• bertujuan • inencipta ahli-ahli kesehatan yang dapat rriernecahkan rnasaalah &darn •berbagai 'situasi. Yang; terutarna bertugas dalam hal ini adalah fakultas kedokteran," yang merupakan bagian 'dari universitas ; dengan dernikian. Keperluan akan tenaga medis yang beraneka ragam akan bertambah, misal- I nya teknisi perawatan ,intensif, jururawat epidemiologi, jururawat tugas luar, insinyur kedokteran, ahli hygiena rumah sakit dsb. Kerja sama dalam regu akan menjadi lebih penting.8. Kedudukan apotheker juga ,akan berubah; farmasi akan lebihdekat ke kedokteran. . .9. Rumah-rumah sakit yang ada sekarang. akan menjadi "kolot" dengan perubahan-perubahan tersehut di atm.' ' • •`10. Exploii informasi akan terjadi terus, sehingga peranan pusat-pusat informasi akan sangat penting. Perkembangan ilrnu pengetahuan alarn. dan filsafat pasti akan mempengaruhi pulaunia kedokteran.Semua hal yang tersehut tadi man tak matt harus dihadapi oleh lembagaIembaga• pendidikan kesehatan. Taritangan itu menjadi lebih besar, oleh karena tenaga-tenaga clan' fasilitas-fasilitas pendidikan kedokteran sangat terbatas di negeri kita. - • - • •• • •Tiga hal penting inerupakan fokus dalam pembinaan fakultas kedokteran, yaitu kedokteran korninunitas, moderniiasi klinik d.an pendewasaan ilnau-iImu kedokteran dasar, untuk menghadapi 'tantangan-tantangan tadi. Membangun salah satu tiang saja dariketiga tiang itu; pasti akan menimbulkan kepincangan.Fakultas Kedokteran UniVersitas Gadjah Mada telah berusaha niem- , Â·bangun jawaban terhadap tantangan tadi, tetapi karni cukup sadar bahwajalan masih sangat jauh. Distribusi alumni kami di seluruh tanah air agak .•menggembirakan, clemikian pula vertikalnya. Program kedokterankommunitas sudah beberapa Winn ditaksanakan dan terus mencoba mencari bentuknya yang tepat. Pengan fasilitas laberatoria dan rumah sakit yang terbatas, jumlah .dosen yang belum memadai dan biaya per mahasiswa per tahun yang rendah kami ter-us berusaha meningkatkan effisiensi dan. produktivitas. Pada akhirnya berhasilnya suatu .fakultas• kedokteran harus diukur pada perbandingan modal yang dituangkan Ice dalam proses pendidikan dan manfaat yang .diberikan oleh produknya-bagi kesehatan massa rakyat di seluruh tanah air meialtii . masa. - •tugas ini terletak dalain •lingkurtgan Departemen Pendidikan dan Kebudayaan. Sebagai bagiari suatu perguruan tinggi, maka Tridarma berlaku pula bagi fakultas kedokteran : Periama, mengajar para mahasiswa untuk pada suatu waktti dapat berdiri sendiri memecahkan masaalahmasaalah kesehatart.• Oleh- karena, perulidikari Mernpersiapkan mahasiswa untuk masa depan, maka 'perididikari haruS berorientasi ke depan, harns diSeSuaikart dengan keadaan yang diharapkan akan terdapat di masa depan. Oleh karena masa , depan yang jauh sukar dirarnalkan dengan. , tepat seluruhuya, maka mahasiswa dipersiapkan untuk dapat 'memecahkan soal-soal baru, menghadapi situasi baru.:„Karena pendiciikan difakultas kedokteran /amanya 6-7 tahun dan seseorang diharapkan meneapai, puncak' produktivitasnya 10-15. ,tahun sesudah .tammat, maka mahasiswa -barns dipersiapkan .:untuk :16722 tahunyang, akaa datang ( Jacob,, 1. .Kedua,' fakultas-kedokteran bertugas Melaltukan penelitian, Mengebbang kan ilmu-ilmu ,kedokteran jadi mencoba memecahkan Masalah masa depan yang, lebih jauh daripada mendidik. Seperti. dalam mengajar, masa depan ini juga relatif. Dalam mengajar makin tinggi tingkatsi mahasiswa, makin dekat dengan,keadaan, sekarang dia dididik. Pada tahapan koassisterisi dia dilatih merawat pasien•pasien ,,sekarang. -Dalam penelitian dalam klinik persoalan-persoalan yang lebih dekat dengan sekarang terutarna mendapat perhatian. HMO-HMO dasir'kedaterari penelitian lebih mengarah ke masa depan yang jauh,. sehingga; bagi mereka yang melihat dekat, relevansinya tidak kelihatan. Bagi mahasiswa-mahasiswa tingkat, rendah kadang juga tidak kelihatan relevansi pelajaran yang diperolehnya dengan persoalan kesehatan atau praktek kedokteran sekarang. Demikian puba jika, penelitian makin dekat ke pengembangan dalam spektrum penelitian, Makin dekat pulakita dengan keadaan sekarang ; sebaliknya ke penelitian murni, makin jauh kita ke masa depan., Ketika dalam pelayanan umum (public service) fakUltas kedokteran terntaa berftinksi' di tingkat klinik; meikipuri pada tingkat pre dan paraklinik pelayanan umum juga dilakukan.
Identification and test of active protein resemble Ribosomr-inactivating proteins (RIPs) on Kaemferia rotunda Linn Wiryatun Lestariana Wiryatun Lestariana
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 35, No 4 (2003)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

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Abstract

Background: Statistically data of US Mortality showed that percentage cause of the death of the cancer is in second place after the heart diseases. Indonesia, Java especially Daerah Istimewa Yogyakarta, the data of The Dr. Sardjito hospital showed that the patients of cancer was increasing in the last year. Indonesian, especially Javanese, uses white turmeric to prevent and to treat cancer. There are some varieties of turmeric available commercially such as Curcuma (Cl mangga Val & Jijp, C. zeodaria and Kaemferia (K) rotunda Linn. The studies showed that C. mangga contain proteins compound that resembles Ribosome-inactivating proteins (RIPs) which have activity to cleave supercoiled DNA. In vitro study indicated that the addition of crude extract of C. mangga on cancer cell-lines (B-LCL, EBV cells and Raji cell-lines) and normal lymphocytes, the percentage of cytotoxic effect on those cancer cell-lines were higher significantly than normal cells.Objective: The aim of the study was to know the presence of RIPs activity in K. rotunda Linn by the ability of the RIPs in cleaving the supercoiled DNA.Methods: The rhizomes of K. rotunda Linn that was in part dried at 40°C. Both wet and dried rhizomes are pounded and then extracted. The resulting crude extract was precipitated to obtain its protein fraction. The crude extract and protein with various concentrations were incubated with the supercoiled DNA and agarose gel electrophoresis was used to test its activity in cleaving the supercoiled DNA. The activity test was done by observing 3 criterions, viz. the thinning of the supercoiled DNA, the circular band thickening and the appearance of the linear band which were subsequently compared to the plasmid DNA without treatment.Result: The results showed that both crude extracts and proteins of both wet and dried samples were able to cleave supercoiled DNA into circular and linear form. The increasing concentrations of the crude extract and the protein resulted in increasing the activity which was indicated by the thickening of circular band and the appearance of the linear band .Conclusion: Kaemferia rotunda contains proteins compound that resemble Ribosome-inactivating proteins (RIPs).which have ability to cleave supercoiled DNA to be circular and linear DNA.Key words: ribosome-inactivating proteins (RIPs) - supercoiled DNA - circular DNA - linear DNA - Kaemferia rotunda Linn
Current issues in Colorectal Cancer Marijata Marijata
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 31, No 04 (1999)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

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Abstract

Background: Three issues about colorectal cancer are now blooming among surgeons and oncologists. The first issue is trend the younger the age of the patients suffering from colorectal cancer. The second is the younger the age of the patients the higher malignant of the cancer. The third issue is the delay of the management of colorectal cancer.Methods: A retrospective study on the colorectal cancer patients who were managed in Patmasuri Surgery Hospital, Yogyakarta, during a 10-year period (1989 - 1998).Results: The first and second issues were not proved, but the third issue was proved. The cause of delay came from the patient and the physician in establishing the diagnosis and in considering the operative procedure.Key words : colorectal cancer - younger age - higher malignant
ytotoxic effect of n-hexane insoluble fraction obtained from kloroform extract of kembang bulan leaves [Tithonia diversifolia (Hemsley) A. Gray] on HeLa cells and mechanism detection by Hoechst 33342 Mae S.H Wahyuningsih, Rul Afiyah Syarif, Zany sari Vindawati, Rita Rakhmawati
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 39, No 03 (2007)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

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Abstract

Background: Tithonia diversifolia (Hemsley) A.Gray is a plant traditionally used to cure various diseases. The previous study indicated that n-hexane insoluble fraction of chloroform extract of T. diversifolia's leaves showed cytotoxic effect on Hela cells. Separation of n-hexane insoluble fraction into several fractions was conducted in order to simplify the components. Therefore, compounds responsible for cytotoxiceffect and their mechanism of action can be determined. .Objectives: To determine fraction having the most cytotoxic effect and its mechanism of action obtained from n-hexane insoluble fractions of T.diversifolia leaves on Hela cells.Materials and methods: Fractionation of n-hexane insoluble fraction from chloroform extract of T. diversifolia leaves was done by using vacuum liquid chromatography. By MTT assay, the cytotoxic effect of fractions on Hela cells in vitro was determined. and doxorubicin was used as positive control. Each group was tested for 3 times In = 3) and the growth inhibition percentage of Hela cells was counted. The data was analyzed by probit regression, and mechanism of action of the active fraction is conducted by staining with hoechst 33342.Results: Fractionation gave five fractions IF1-5). The ICso levels obtained of those fractions were : F1 having ICso = 32.168 :!: 12.513; F2 = 11.416 :!: 0.535; F3 III = 6.223 :!: 1.303; F4 = 94.308 :!: 7.843; and F5 = 41.740 :!: 6.381Ig/ml; while the ICso value of doxorubicin was 0.241 :!: 0.170 Iglml. Conclusions: Fraction 3 of the n-hexane insoluble displayed the highest cytotoxic effect on Hela cells with ICso= 6.223:!:: 1.303 Iglml. Fraction 3 at the concentration of 3.93 uglml caused chromatin condensation and fragmentation of DNA on Hela cells.Key words: T. diversifolia, cytotoxic effect. Hela cells, insoluble n-hexane fractions
Liver abnormalities in typhoid fever in children Triwibowo Soedjas Triwibowo Soedjas
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 15, No 03 (1983)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

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Abstract

Fifty two patients with typhoid fever were studied. Hematomegaly occurred in 7I % of the patients. Hepatic enlargement was noted in the first week as well as during the course of the disease but the maximum incidence was found in the second week. Almoimal liver function tests were found in both groups, with and without hepatomegaly. Jaundice was present in 2 cases.Biopsies showed focal necrosis, cellular infiltration. Kupffer cell proliferation and hydropic degeneration. Typhoid nodules were demonstrated in 2 out of the 7 cases.The prognosis of typhoid fever with liver abnormalities is good and requires no further treatment.Key Words: hepatomegaly - abnormal liver function test - jaundice - liver biopsy - nonspecific reactive hepatitis
Nonspecific Urethritis(NSU) or Non-Gonococcal Urethritis(NGU),The Biggest Problem In Sexually Transmitted Diseases(STD) A Literature Study Soedarmadi Soedarmadi
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 7, No 04 (1975)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

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Abstract

Not adequately realized by the practicing physician is that at least half of the cases of urethritis seen in practice are not due to gonococcus and will not respond to penicillin, but will respond to tetracyclin.The etiology of NSU is not clear yet, but the role of infective agent (or agents) is clear. The diagnosis is made by exclusion.Despite the lack of agreement concerning the aetiology, the treatment is relavely simple, and tetracycline is the drug of choice.
Secondary sexual characteristics development pattern in elementary school male students in the municipality of Yogyakarta Daniel Susatyo Wirawan Daniel Susatyo Wirawan
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 34, No 03 (2002)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

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Background: Twenty two point two percent of Indonesian population are adolescents, and 50.9% of them are male. The development characteristics during puberty are growth spurt, sexual maturation, and secondary sexual development, and these characteristics are affected by nutritional status, health care and social economic status.Objectives: The aim of this study was to investigate the correlation between nutritional status and social factors with the development of secondary sexual characteristics, sexual maturation, and the average age of spermache in YogyakartaDesign: Cross sectional studyMaterial and Methods: One thousand two hundred and sixty three male students enrolled using a proportional population multi stage random sampling were included in this study from all elementary school students in the Municipality of Yogyakarta. Nutritional status was assessed by z-score for weight for age, height for age, and body mass index (BMI). The age of spermache, secondary sexual characteristics, and social factors was obtained by standard questionnaires.Result: Based on z-score value, most of the subjects were wellnourished (84.3%), and normal height (85.3%). Based on BMI most of the students were wasted (62.8%). The average age of spermache in wellnourished, and normal height students were younger than those undernourished, and this difference was statistically significant (p < .001). There was no statistically difference in the development of secondary sexual characteristics between wellnourished and undernourished students, either based on z-score or BMI. Most of the subjects who were exposed to pornography, either pictures (OR 2.20 95% CI 1.31-3.69) or videos (OR 2.09 95%Cl 1.29-3.38) had more spermache than those without experienced. Most of the students (71.74%) were in level 1 of sexual maturity rating (SMR1) based on Tanner scale. There was a relationship between nutritional status based on BMI and sexual maturity rating (p= .007). Conclusions: Nutritional status influenced spermache, and earlier age of spermache, also sexual maturity. Pornography exposure affected spermache.Key words: male students - secondary sexual characteristics - spermache - nutritional status - pornography
Filariasis caused by nonperiodic form of Brugia malayi among school children in the settlement of Dayak indigenous people and transmigrants in East Kalimantan FA Sudjadi FA Sudjadi
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 31, No 01 (1999)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

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Background: New filarial worm subspecies, i.e. nonperiodic form of Brugia malayi Lichtenstein, was recently reported from East Kalimantan in addition to the previously known subperiodic and periodic form of the species, which was endemic in some rural areas in Indonesia.Objectives: (1) To search new endemic areas of filariasis in the indigenous people and newcomers. (2) To know current status of the disease among schoolchildren in the study areas.Subjects: Schoolchildren in the settlement of Dayak indigenous people (Krayan and Kayungo villages) and transmigrants (Petung UPT: Girimukti and Petung villages), belonging to Long Ikis and Penajam subdistricts, the district of Pasir.Methods: Based on the microfilarial character of aperiodic, blood examinations by simple or concentration method on schoolchidren were carried out at day time within school hours in 1987/1988.Results: (1) Endemic areas of the B. malayi subspecies were discovered, not only in the villages where the schools were situated, but their vicinities as well, i.e. the school children's home villages. The other schoolchildren's home villages found as endemic areas were Jemparing, Nipaulo, Olung, Sepingan around the Dayak settlements and Lawe-lawe, Tanjung Jumlai, Salakloang, Tunan around the UPT of Petung. (2) Higher microfilaremia rates were found in schools in Krayan (26,0% or 52/200 children) and Kayungo (14,8% or 16/108 children). In the transmigrant settlement, although the concentration methods of examinations were adopted, lower microfilaremia rates were recorded such in schools in the villages of Girimukti (6,9% or 34/490 children) and Petung (3,5% or 33/950 children).Conclusions: Day-time blood survey at school was proved to be a simple method to discover endemic areas of the nonperiodic form of B. malayi in East Kalimantan.Key words: B.ma/ayi - nonperiodic form - daytime examinations - school children
Activity of the combined pyrimethamine and sulphadoxine as antifilarial in Brugia pahangi - Aedes togoi model infection Sugeng Juwono Mardihusodo Sugeng Juwono Mardihusodo
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 22, No 01 (1990)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

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The mortality rates of Aedes togoi mosquito either uninfected or infected with Brugia pahangi maintained in insectary for 12 days with various concentrations of the combined pyrimethamine (P) and sulphadoxine (S) in 10% sugar-water solution: 5 mg% P + 100 mg%, 10 mg% P + 200 mg% S, and 20 mg% P + 400 mg% S significantly increased compared to that of the control groups. The present evidence showed a definite insecticidal action of the combined drugs.Infection and infective rates of Ae. togoi mosquitoes, average number of B. pahangi larvae and infective larvae per infected mosquitoes, average size and movement of the larvae in the groups of mosquitoes treated with various concentrations of the combined drugs for 12 days decreased significantly compared to that of the control groups of mosquitoes. The present evidence demonstrated a definite action of the combined pyrimethamine and sulphadoxine as an antifilarial in B. pahangi Ae. togoi model infection.Key Words: pyrimethamin-sulphadoxine - Brugia pahangi - Aedes togoi - antifilarial - insecticide
Secretion of a Novel Gene Product through Micro-encapsulated Recombinant Cells a Preliminary Report M. Mansyur Romi M. Mansyur Romi
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 26, No 03 (1994)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

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Galur sel hasil rekayasa genetik yang berada dalam kondisi terisolasi secara imunologis diharapkan dapat diimplantasikan kepada beragam penerima. Dengan demikian tersedia strategi altematif bagi terapi gena, yang umumnya bergantung kepada modifikasi genetik terhadap sel dari penderita sendiri. Penelitian ini mengamati ekspresi hormon pertumbuhan manusia (HPM) dari hasil rekayasa genetik sel mencit yang ditempatkan di dalam membran imunoprotektif secara in vitro.Sel mencit Ltk" ditransfeksi dengan plasmid pNMG yang mengicode gena HPM dan gena resistensi terhadap G418. Sel-sel yang mensekresi HPM dalam jumlah banyak dipilih untuk dibungkus dengan mikrokapsul berdiameter 300nm yang terbuat dari bahan alginate — polylysine — alginate. Prosedur pengkapsulan tidak mengganggu kelangsungan hidup sel, terbuicti bahwa lebih dari 95% sel masih tetap hidup seusai pengkapsulan. Sepanjang percobaan selama 3 minggu angka sel yang hidup dapat dipertahankan sekitar 90%, jumlah sel di dalam mikrokapsul meningkat lebih dari sepuluh kali lipat, dan HPM di dalam media juga meningkat konsentrasinya. Membran mikrokapsul cukup permeabel, dari perhitungan pada awal percobaan lebih dari 80% HPM yang disekresi dapat berdifusi ke dalam media. Dapat disampaikan bahwa produk gena baru dari galur sel baku hasil rekayasa genetik dapat dihantarkan melewati mikrokapsul dari bahan alginate-polysine-alginate secara in vitro paling tidak selama tiga minggu. Selain itu, prosedur yang dikerjakan cukup memadai bagi kelangsungan hidup sel, lingkungan dalam mikrokapsul dapat mendukung pertumbuhan sel, dan membran kapsul bersifat permeabel bagi produk gena baru.Key words: genetically modified cells-plasmid pNMG-human growth hormone-immuno protection-microcapsule

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