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Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

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Published by Universitas Gadjah Mada

ISSN : 01261312 EISSN : 23563931 DOI : http://dx.doi.org/10.19106/JMedSci005303202101

Core Subject : Science,

Immunology & microbiology Neuroscience

Journal of the Medical Sciences (JMedSci) or Berkala Ilmu Kedokteran (BIK) is an international, open-access, and double-blind peer-reviewed journal, published by Faculty of Medicine, Public Health and Nursing, Universitas Gadjah Mada Yogyakarta Indonesia. JMedSci aiming to communicate high-quality articles in the areas of biomedical science from basic to clinical sciences.The journal welcomes papers from original articles, case reports, reviews, and book reviews. All papers published in JMedSci are freely available as downloadable pdf files. The journal began its publication on March 1973 and published quarterly (January, April, July, and October). JMedSci is abstracted and indexed in DOAJ, Crossref, Google Scholar, Sinta, Indonesia One Search. JMedSci is accredited by Directorate of General Higher Education, the Ministry of Research, Technology, and Higher Education, Indonesia

Arjuna Subject : Kedokteran - Kedokteran (Lain-Lain)

Articles 2,170 Documents

163 164 165 166 167

The Expression of Homo Sapiens microRNA-21 (Hsa-miR-21-5p) and mRNA Reversion Inducing Cysteine Rich Protein with Kazal Motifs (RECK) in Plasma of Epithelial Ovarian Cancer Aprilia Indra Kartika; SN Chasanah; AS Fitriawan; DS Tanjung; MS Ftria; FK Pakun; R Oktriani; A Trirahmanto; H Prajatmo; T Aryandono; SM Haryana
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 48, No 4 (2016): SUPPLEMENT
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

ABSTRACT Epithelial Ovarian Cancer (EOC) is malignant cancer that caused death for most women in Indonesia. The emergence of EOC showed no specific symptoms in its early stages; that makes the screening mostly occur when patients are in advanced stage. Treatment of EOC at an advanced stage will be more challenging with poor prognosis. Therefore, minimally invasive biomarkers are needed to diagnose at the early stage. microRNA is one of the potential biomarkers which not only expressed inside the cell but also secreted outside the cell with exosome protection. This protection makes microRNA stable. Moreover, several studies have shown the ability to detect microRNA in the blood sample. microRNA-21 (miR-21) is oncomiR which targeted tumor suppressor mRNA RECK based on in silico analysis.The first aim is to determine the expression of miR-21 in plasma samples of EOC patients compared with healthy controls. The second aim is to investigate the expression correlation between miR-21 and RECK mRNA.Blood samples were collected from 30 patients and 30 healthy controls. Plasma was then obtained from centrifugated blood samples. The total RNA was isolated and reverse transcribed to produce cDNAs. cDNAs were then quantified using qPCR using specific primer for miR-21 and RECK mRNA. The expression analysis was done relative expression method by Livak. The expression of miR-21 was calculated using the miR-16 expression as the reference gene. Also, Beta-actin was used as reference gene for RECK mRNA calculation. The correlation between the expression of miR-21 and mRNA RECK was analyzed using the Spearman rho correlation analysis.In this study showed the expression of miR-21 in patients with EOC increased 4.7579-fold compared with healthy controls (p <0.05). On the other hand, the miR-21 target, RECK mRNA, decreased 4,2 times with fold change 0.237665 on plasma EOC patients compared with healthy controls (p <0.05). The statistical calculation of the expression of miR-21 and mRNA RECK was inversely proportional to mRNA RECK with a strong correlationThis study has been able to prove that the expression of miR-21 is up-regulated in EOC patients and confirmed by the down-regulation of RECK expression. The next research challenge is to make anti-miR-21 to suppress the expression of miR-21 in EOC, which can be analyzed the effect of miR-21 in the development of EOC.

Comparison of giving ephedrine 30 μg/kgbw iv and lidocaine 2% 40 mg iv to educe pain induction propofol 2 mg/kgbw iv in general anesthesia . Mujahidin; Bambang Suryono; Yusmein Uyun
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 45, No 01 (2013)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

The administration of propofol often causes burning sensation in the area of injection. There have been efforts to reduce discomfort, but the result remains unsatisfactory. The main purpose of this study is to find objective evidence of whether the administration of ephedrine 30μg/kgBB IV is more effective than 40mg 2% lidocaine in order to reduce propofol 2mg/kgBB injection pain.This study was conducted in the double blind Randomized Controlled Trial (RCT) to 128 patients, with the range of age 18-60 years, physical status ASA I and II, BMI 17.5 – 24.5 kg/m2 which had been done elective surgery in the Intergrated Surgery Center of Dr. Sardjito Hospital Yogyakarta and other education network (Banyumas Hospital, Panembahan Senopati Bantul Hospital, Orthopedi Solo Hospital, Saras Husada Purworejo Hospital) and carried out in 2-4 months, general anaesthesia has been done with endotracheal intubation technic. Subjects have been divided into 2 groups, group A (lidokain 40 mg) and group B (efedrin 30μg/ kgBB). All of the patients have been given at the vena sefalikabasalika with 18G needle and RL. After one of the drugs has been given, the patient will be inducted with propofol 60 seconds later. The measurement of discomfort caused by propofol injection has been marked with discomfort scoring system (score 0-3), the blood pressure measurement has been taken before and after propofol administration. The results showed no statistically significant difference in the two study groups (2% lidocaine 40 mg IV vs ephedrine 30μg/kgbb IV) on pain response measured when patients received propofol 2mg/kgbb IV, p > 0.05 (P = 0.068) In group A there were 52 patients (81.3%), pain-free, while for group B there were 59 patients (92.2%). The pain response obtained was varies, in group A obtained a scale of 1-3, while in group B obtained a scale of 1-2. It can be concluded that administration of IV ephedrine 30μg/kgBB is not a significantly different effect compared with 2% lidocaine 40 mg IV to prevent response to pain during injection of propofol 2 mg / kg IV, P> 0.05 (P = 0.068), but clinically group B have the ability to eliminate pain in 59 patients (92.2%) compared with group A in 52 patients (81.3%), while the possibility of causing pain in group A were 12 patients (7.8%) and group B were 5 patients (18.7%).

Effect of Syzigium cumini (pulp) extract on malondyaldehyde and blood pressure in chronic restraint stress rat Mega Febia Suryajayanti; Anggi Laksmita Dewi; Bira Arumndari Nurrahma; Ayu Dwi Silvia Putri; Zunamilla Khairia; Arta Farmawati
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 49, No 3 (2017)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

ABSTRACTIntroduction. Chronic stress disturbs the equilibrium of oxidant-antioxidant redox in the human body which accelerates cellular aging. This condition promotes earlier onset of age-related diseases. Human body needs exogenous antioxidant to fight stress. Syzigium cumini has high antioxidant activity because it’s rich in anthocyanin. It has potential to become natural antioxidant source thus it needs to be developed.Aim. This study aims to investigate the effect of Syzigium cumini (Pulp) extract on the stress biomarkers which are Malondialdehyde and blood pressure in rat induced by restraint stress.Method. Chronic stress phase is conducted with male Wistar rats (n=20) which were divided into 4 groups (normal control, negative control, and two treatment groups with the ethanol extract of Syzygium cumini (Pulp) at 20 mg/200 gram and 40 mg/200 gram body weight). Restraint stress was carried out by placing the animal in narrow tube for 30 minutes daily (7 days). Blood pressure was evaluated before and after the treatment while Malondialdehyde was evaluated after the treatment. The One-way ANOVA was used to analyze the parameters.Results. Based on chronic stress phase, treatment groups show significant result in preventing the increase in blood pressure (P < 0,001). The increased levels of Malondialdehyde were prevented significantly in all treatment groups (P < 0,001)Conclusion. In chronic stress phase, Syzygium cumini (Pulp) extract can become an antihypertensive agent. Syzygium cumini (Pulp) extract shows an effect in decreasing lipid peroxidation products. This result shows that the Syzygium cumini (Pulp) extract has potent antioxidant function.Keywords. Syzigium cumini, restraint stress, malondialdehyde, MDA, blood pressure

The characterization of NMR signal for blood pressure monitoring system and its testing Bambang Murdaka Eka Jati; . Kusminarto; Agung Bambang Setio Utomo; Bambang Irawan
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 47, No 01 (2015)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

ABSTRACT A blood monitoring system based on NMR method has been designed on constructed. This set-up of equipment used magnetic permanent, radio frequency (RF), receiver coil (RC), function generator (FG), amplifier which included the filter, as well as the oscilloscope digital storage. The background of this research was based on the sensitivity of NMR signal. The signal must be separated from signals background. This method was done by adjusting the frequency on FG, which was connected to radio frequency (RF) coil, on empty sample. Subsequently, NMR signal was received by RC, and that signal could be shown on oscilloscope at resonance condition. The true frequency on NMR signal was Larmor frequency, and the other was background. The two variables of this experiment were the position of RF coil and the location temperature (20 up to 30oC). In conclusion, the resonance frequency of NMR signal (as Larmor frequency) was 4.7 MHz (at static magnetic field of 1,600 gauss) and it could be separated from background signals (3.4 and 6.2 MHz), and that signal was almost constant to room temperature. The equipment was used for sample testing. It gave systole/diastole data of 110/70 mmHg (on sphygmomanometer) that was similar to 17/9 mV (on NMR signal). ABSTRAK Telah dikembangkan alat pemantauan tekanan darah berdasar prinsip NMR.

Ekspresi EBNA-1 pada diffuse large B cell lymphoma serta Small lymphocytic lymphoma tipe nodal dan ekstranodal . Indrawati; . Irianiwati; . Harijadi
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 38, No 01 (2006)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

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EBNA-1 expression in nodal and extranodal type of diffuse large B cell lymphoma and small lymphocytic lymphoma Background : Diffuse large B cell lymphoma is the most common type of non-Hodgkin lymphoma. Epstein-Barr Virus infection has been suggested to be involved in the pathogenesis of non-Hodgkin lymphoma. Objective : To know the expression of EBNA-1 in diffuse large B cell lymphoma and small lymphocytic lymphoma and its association with nodal and extranodal location of B cell non-Hodgkin lymphoma Methods : Thirty five cases of B cell non-Hodgkin lymphoma from embedding parafin tissue were constituted the basis of the study. Immunohistochemical examination using CD20 and EBNA-1 monoclonal antibody was done. The difference of EBNA-1 expression between diffuse large B cell lymphoma and small lymphocytic lymphoma and the difference of EBNA-1 expression between nodal and extranodal locations of B cell non-Hodgkin lymphoma were analized by chi square test Result and Conclusion : Sixteen cases of B cell non-Hodgkin lymphoma showed positivity of EBNA-1 expression. There was significant difference between the number of cases with positivity of EBNA-1 expression in nodal and extranodal location of B cell non-Hodgkin lymphoma. There was not significant difference between the number of cases with positivity of EBNA-1 expression in diffuse large B cell lymphoma and small lymphocytic lymphoma. The result supported that latency EBV related lymphoma frequently occurs in extranodal location Key words : EBNA-1, diffuse large B cell lymphoma, small lymphocytic lymphoma, nodal, extranodal

ROLE OF DNA METHYLATION AS A DIAGNOSTIC BIOMARKER OF SPORADIC BREAST CANCER Wirsma Arif Harahap
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 48, No 4 (2016): SUPPLEMENT
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

The initiation and progression of breast cancer have been recognized for many years to be secondary to the accumulation of genetic mutations which lead to aberrant cellular function. Genetic mutations, either inherited or sporadic, may result in the activation of oncogenes and the inactivation of tumor suppressor genes. The more recent discovery that reversible alterations in histone proteins and deoxyribonucleic acid (DNA) can also lead to tumorigenesis has introduced a novel term to the field of cancer research: epigenetics. Epigenetics refers to the study of heritable changes in gene regulation that do not involve a change in the DNA sequence. The most often studied in epigenetics of breast cancer is DNA methylation. That a promoter methylation result in transcription blockade supports the notion that cellular inhibition takes place. Compared to normal tissues, hypermethylation occurs from double to triple in cancerous ones. DNA methylation plays a crucial role in oncogenesis and is one of the hallmarks of cancer.Detection of aberrantly methylated CpG islands in promoter region of several genes in DNA sample derived from nipple aspirates, serum, or cancer tissue associated with down regulation of expression or loss of function of these genes has been associated with early stages of breast cancer, where hypermethylation of CpG island points to poorer prognosis in breast cancer. DNA methylation has been identified as signature for TNBC. Methylation of BRCA1 gene is frequently demonstrated in young, estrogen receptor-negative breast cancer patients. Methylation of specific genes is known to differ across race and socioeconomic status. BRCA1 methylation in premenopausal women with sporadic breast cancer in West Sumatra region has been higher than in Western women.DNA methylation may be used to enhance current breast cancer classification. There is such a distinction between methylation and gene expression profiles of breast cancer that not all methylation profiles fit within the same molecular subtype. Specific gene methylation profiles are identified for basal-like, luminal A and HER2-overexpressing breast cancers. A number of studies have analyzed the methylation status of BRCA1, a key player in TNBC. One study demonstrated that BRCA1 promoter was methylated in TNBC. It was discovered that the sensitivity of TNBC cell lines to PARP inhibitors was increased when BRCA1 was methylated. Concurrently, BRCA1 methylation quantity was higher in patients with complete response than in those who are non-responders of neoadjuvant chemotherapy.Epigenetics is now the cutting edge of cancer research. Advances in this field will have major implications in diagnosis, prevention, treatment of cancer, and formulation of new epigenetically targeted cancer drugs.

Relationship of Geriatric Nutritional Risk Index (GNRI) with length of hospitalization and mortality rate in elderly patients Retno Ambarukminingsih; I Dewa Putu Pramantara; Neneng Ratnasari
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 46, No 01 (2014)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

The proportion of elderly population is growing faster than any other age group. Malnutrition isa widespread problem in elderly and has been recognized as the most common cause of mortalityand morbidity. Mini Nutritional Assessment (MNA) is recommended to detect malnutrition riskamong geriatrics. However, it is just appropriate for geriatric who stay at home. For hospitalizationpatients, Nutritional Risk Index (NRI) that using albumin and weight is recommended. However,the weight data is often not possible obtained in geriatrics. Therefore, Geriatric Nutritional RiskIndex (GNRI) using ideal body weight is recommended. The aim of this study was to evaluaterelationship of GNRI with length of hospitalization and mortality rate in geriatric patients. Thiswas an observational study with a prospective cohort design conducted in Dr. Sardjito GeneralHospital, Yogyakarta from January to February 2012. Patients who met the inclusion and exclusioncriteria were recruited. Laboratoty and clinical examinations as well as GNRI scoring were thenperformed. Patients were grouped into two groups i.e. patients with GNRI score < 82 and e”82. The patients were then monitored during hospitalization until they were discharged due todie or recovered. The length of stay and patients died were then recorded. The results showedthat the length of stay of patients with GNRI score < 82 (14.32 ± 8.20 days) was significantlylonger than those with GNRI score 82 (9.31 ± 6.15 days) (p = 0.006). Moreover, the mortalityrate of patients with GNRI score < 82 (42.1%) was significantly higher than those with GNRIscore 82 (2.8%) (p = 0.000). Kaplan-Meier survival analysis showed survival rate of patientswith GNRI score <82 rapidly decreased when compared with those with GNRI score 82. Inconclusion, there is negative correlation between GNRI with length of hospitalization and mortalityrate in elderly.

Mammographic Density and Estrogen Receptor α Gene Polymorphism in Javanese Women Lina Choridah; Teguh Aryandono; Arif Faisal; Ahmad Hamim Sadewa; Dewajani Purnomosari
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 49, No 2 (2017)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

Estrogen plays important roles in breast cancer as it binds its receptor in breast tissue. The most studied variants in estrogen receptor α encoded by ESR1 gene are the ESR1 PvuII and XbaI polymorphisms, which were associated with lower sensitivity to estrogen. We determined the proportion of ESR1 XbaI and PvuII polymorphisms in Javanese woman in Yogyakarta, Indonesia and analyzed the correlation between genetic variations with mammogram density. ESR1 XbaI and PvuII polymorphisms of 50 cases and 58 controls were identified using PCR-RFLP. Breast density was assessed based on digitizer mammograms. Quantitative analysis was performed using an interactive program based on cumulus of two thresholds. Mean of density and frequencies of SNPs were compared between cases and controls to identify the association between SNPs and cancer susceptibility. Mammographic density was significantly higher in cases (52%) than controls (0.41%) (p < 0.05). Women with one or two copies of the PvuII T allele and XbaI A allele had higher mammographic density compared with women with C and G alleles, respectively. The proportion between PP and TT genotype was not statistically significant (p > 0.05), while the proportion between AA and GG was significantly different (p < 0.05). Haplotype 2 (CG/PX) was associated with lower sensitivity to estrogen and reflects a decrease of mammographic density. These findings were consistent with other studies that showed that ESR1 polymorphisms may affect breast cancer risk through differences in breast density.

Antimicrobial activity of bioactive compounds isolated from Swietenia mahagoni (L) Jacq. against Staphylococcus aureus and Pseudomonas aeruginosa Handry Darussalam,; Titik Nuryastuti; . Mursiti; . Mustofa
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 46, No 04 (2014)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

Widespread bacterial resistance has led to more difficult to treat infectious diseases with availableantibiotics. Therefore, new antibiotics are needed face of the growing antibiotic resistance. Swieteniamahagoni (L.) Jacq. is one of potential medicinal plants as a source new antibiotics. Five compoundshave been isolated from an ethanolic extract of S. mahagoni (L.) Jacq., however its antimicrobialactivity has not been investigated, yet. This study was conducted to evaluate the antimicrobialactivity of these compounds. Minimal Inhibitory Concentration (MIC) and Minimal BactericidalConcentration (MBC) were determined against Staphylococcus aureus and Pseudomonas aeruginosastrains. Among five compounds tested, compound 3 (3,4,5,6,7-pentaethyl-1-methoxy-1H-indazole)and compound 4 (5-ethyl-6-methoxymethyl-2-methyl-1,2-dihydropyridine) were found to be activeagainst the bactrial strains tested with the MICs and MBCs values ranged from 50 to 100 μg/mL. Inconclusion, among five compounds isolated from S. mahagoni (L.) Jacq., compound 3 and 4showed moderate antimicrobial activity against S. aureus and P. aeruginosa strains.

The quality of live of patients’ allegic rhinitis after posterior nasal neurectomy at Dr Sardjito General Hospital Yogyakarta, Indonesia 2016 Wahyu Dwi Kusdaryanto; Windarti Isminarsih; Luh Putu Lusy Indrawati
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 50, No 1 (2018): SUPPLEMENT
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

Allergic rhinitis (AR) is disease by incidence and prevalence of high and is expected to rise to load against the economy and lowering the quality of life of patients.with AR involved many factors and an impact of highly significant on the quality of life and affect the economy. The quality of life patients done to assess the effects and result of surgery.Sino nasal outcome test-20 ( snot-20 ) was one of the instruments specific measurement the quality of life of patients AR. 10 patients who surgery action , obtained improving the quality of life based on snot- 20 observed 28 days after done the act of surgery. Rate the SNOT-20 before surgically obtained 37,3 with DS ± 7,87 and 28 days after done surgically obtained rata-rata snot-20 is 6 with DS ± 2,49. The SNOT-20 pre and post action there are different of significant (p = 0,024 ) .

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Journal Mail Official jmedscie@ugm.ac.id

Editorial Address https://jurnal.ugm.ac.id/bik/about/editorialTeam

Location Kab. sleman, Daerah istimewa yogyakarta INDONESIA

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Contact Name
Nurhadiyahya

Contact Email
nurhadiyahya@ugm.ac.id

Phone
+6289672800034

Journal Mail Official
jmedscie@ugm.ac.id

Editorial Address
https://jurnal.ugm.ac.id/bik/about/editorialTeam

Location
Kab. sleman,

Daerah istimewa yogyakarta

INDONESIA