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Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

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Published by Universitas Gadjah Mada

ISSN : 01261312 EISSN : 23563931 DOI : http://dx.doi.org/10.19106/JMedSci005303202101

Core Subject : Science,

Immunology & microbiology Neuroscience

Journal of the Medical Sciences (JMedSci) or Berkala Ilmu Kedokteran (BIK) is an international, open-access, and double-blind peer-reviewed journal, published by Faculty of Medicine, Public Health and Nursing, Universitas Gadjah Mada Yogyakarta Indonesia. JMedSci aiming to communicate high-quality articles in the areas of biomedical science from basic to clinical sciences.The journal welcomes papers from original articles, case reports, reviews, and book reviews. All papers published in JMedSci are freely available as downloadable pdf files. The journal began its publication on March 1973 and published quarterly (January, April, July, and October). JMedSci is abstracted and indexed in DOAJ, Crossref, Google Scholar, Sinta, Indonesia One Search. JMedSci is accredited by Directorate of General Higher Education, the Ministry of Research, Technology, and Higher Education, Indonesia

Arjuna Subject : Kedokteran - Kedokteran (Lain-Lain)

Articles 2,170 Documents

169 170 171 172 173

Impulsivity and novel object recognition test of rat model for vascular cognitive impairment after antipsychotics treatment Ronny T Wirasto; Irwan Supriyanto; Arif Prassetyo; Nunu N Madjid; Susini R Sari; Baiq R Rhadianaa; Heru Yulianto; Nur Arfian; Nyoman Kertia
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 48, No 3 (2016)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

ABSTRACTVascular cognitive impairment (VCI) is a common condition in which no standard treatment has been approved. VCI is often accompanied by behavioral problems which require psychiatric interventions. The common therapeutic agent used for the acute management is antipsychotic injections. Current findings showed that atypical antipsychotic possess better safety profile for treating behavioral problems related to VCI compared to typical antipsychotic. In this study, we induced VCI in Sprague Dawley rats between 6-8 weeks old using bilateral carotid communist artery occlusion technique. The subjects were divided into 4 treatment groups: sham, olanzapine, haloperidol, and risperidone groups. Subjects received intramuscular injections of subsequent drugs for 3 days post VCI induction. Impulsive behavior and object recognition were examined using cliff jumping test and novel object recognition test. The analyses results showed that impulsive behavior was lower in the olanzapine and haloperidol groups compared to sham group, although it was not statistically significant (p = 0.651). The results also showed that there were no significant differences in the time spent exploring old and novel objects in all groups (p = 0.945;0.637 respectively). In conclusion, antipsychotic injection might not be effective to control impulsive behavior post VCI induction.

Oncoplastic breast surgery in Asia Ava Kwong
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 48, No 4 (2016): SUPPLEMENT
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

Oncoplastic breast conservation surgery has given a new light to the options of performing breast conserving surgery by combining oncological principles of breast cancer surgery with plastic surgery techniques. The ultimate oncoplastic achievement would be the conversion of what normally would be considered as an oncologic and/or cosmetic failure when using standard techniques of breast conserving surgery into both oncological and cosmetic success and to avoid mastectomy where possible.Most Techniques are fairly well established. However surgical techniques may also need to be adjusted based on the cohorts that the treatment is offered to. For example, for larger breasts, the technique is more forgiving. However for smaller breast, there is still a limitation of the cosmetic outcome when a high percentage of breast volume has been excised no matter how good the technique or the surgeon is.Various aspects of oncoplastic breast surgery technique will be discussed and its applicability to Asian Cohorts.

Effects of Dioscorea esculenta and Eubacterium rectale on insulin receptor substrate 1 (Irs1) Expression in skeletal muscle and homeostatic model assessment-insulin resistance (HOMA-IR) in diabetic rats . Sunarti; Tri Setyawati; Neni Oktiyani; Rio Jati Kusuma
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 47, No 3 (2015)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

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Low expression of insulin receptor substrate 1 (Irs1) is associated with insulin resistance and type 2 diabetes mellitus (type 2 DM). This study was performed to evaluate the effects of Dioscorea esculenta and Eubacterium rectale on the Irs1 expression in the skeletal muscle and the homeostatic model assessment-insulin resistance (HOMA-IR) of diabetic rats. Twenty-five male Wistar rats were divided into five groups i.e. non diabetic rats Group 1; diabetic rats as Group 2; diabetic rats + D. esculenta as Group 3; diabetic rats + E.rectale as Group 4 and diabetic rats + both E. rectale and D. esculenta as Group 5. Rats were made diabetic with induction of intraperitoneally injection of nicotinamide and streptozotocin. After four weeks of the interventions, the blood and skeletal muscles were taken. The Irs1 expression was analyzed with immunohistochemical staining, plasma glucose levels was analyzed using a spectrophotometer, and insulin was analyzed using ELISA methods. All intervention groups reduced plasma glucose levels and HOMA-IRs (p<0.001) and increased Irs1 expression. The greatest reduction of plasma glucose levels and increase of Irs1 expression in the skeletal muscle were found in Group 4, however, the lowest of HOMA-IR was seen in Group 5. These results suggested that D.esculenta, E.rectale, and the combination reduced plasma glucose levels and HOMA-IR by increasing Irs1 expression in skeletal muscle.

Structural evaluation and animal implantation of porous eggshell wastederived hydroxyapatite graft as bone substitution Yudha Mathan Sakti; Rahadyan Magetsari
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 45, No 04 (2013)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

The development of hydroxyapatite graft with high economically value is needed for orthopedic practice in developing countries. Eggsell waste is well known as natural substance for calcium resource. It has been used as raw material in producing hydroxyapatite. This study was conducted to synthesize porous hydroxyapatite from eggshell waste and evaluate its activity as bone substitution. The porous hydroxyapatite graft was manufactured from eggshell and sugar as a raw material using hydrothermal process. The porous eggshell waste-derived hydroxyapatite (EW-HAP) graft was characterized using X ray difractometer (XRD) and analytical scanning electron microscope (SEM) and compared with commercial hydroxyapatite (HAP) JCPDS 09-432 graft (Bangros®) as standard. The porous EW-HAP graft obtained was then implanted on critically sized femoral defects surgically created in the right thigh of male Wistar rats (Rattus norvegicus) with Bangros® as control. Radiological examination using XRD and histological examination using hematoxyline-and-eosin staining of the bone femour were performed at 28 days after implantation. The results showed that the XRD pattern for EW-HAP was likely similar with the HAP standard. However, the SEM examination showed that the pasticle size of EW-HAP graft (2.5-3 μm) was higher than those HAP standard graft (1.5-2 μm). Radiographs according to the International of Limb Salvage (ISOLS) radiological evaluation system between EW-HAP graft (6.1 ± 1.45) and HAP control graft (6.9 ± 2.10) was not significantly different (p>0.05). Moreover, histological examination according to Lane and Shandu scoring system between the both graft (4.0 ± 0.94 versus 4.4 ± 0.92) was also not significantly different (p>0.05). It can be concluded that the structure EW-HAP graft is similar with HAP graft standard. The both grafts have also equal outcome as bone substitution.

Variation of the breast cancer susceptibility marker, rs4245739, is associated with differential miRNA binding and MDM4 expression Sumadi Lukman Anwar; Angel Carlos-Roman; Wahyu Wulaningsih; Johnathan Watkins
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 48, No 4 (2016): SUPPLEMENT
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

A polymorphism, rs4245739, has been associated with susceptibility of several cancers including ER-negative breast cancer. Rs4245739 is located at the 3’UTR of MDM4 gene, an oncogene that negatively regulates p53. The polymorphism has been associated with binding changes of miR-191. We studied, the influence of SNP rs4245739 to the binding of microRNAs, expression of microRNAs and MDM4. Using FindTar software, we detected potential microRNAs affected by the SNP-flanking sequence. We then used RNA sequencing data from ER-negative breast cancer to compare expression of miR-184, miR-191, miR-193a, miR-378, and MDM4 in different genotypes. Comparison of ER-negative patients with and without expression of miR-191 as well as profile microRNAs (miR-184, miR-191, miR-193a and miR-378 altogether) can differentiate expression of MDM4between different alleles. In addition, the number of lymphatic nodes affected in the individuals was also found to be significantly reduced in the risk group obtained by the miRNA profile method. We show our methods especially miRNA profile approach, are able to obtain new molecular and clinical features related to the rs4245739 SNP, a variant located in the 3’UTR of MDM4 gene and known to appear in different types of cancer. Keywords: ER negative breast cancer, rs4245739, microRNA, MDM4, p53

Cytotoxic Activity of /V-alkyl and /V-benzyl 1,10-phenathroline derivatives in Human Cancer Cell Lines Esti Nurwening Sholikhah Mustofa Isnatin Miladiyah
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 39, No 01 (2007)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

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Background: In our study on the antiplasmodial activity of 1,1 O-phenanthroline derivatives, we found some compounds possessing a potential cytotoxic in normal cell line.Objective: In this study we tested these derivatives in human cancer lines in order to know their in vitro anticancer activity.Materials and methods: Six derivatives of 1,1 O-phenanthroline, 4 derivatives of N-alkyl and 2 derivatives of N-benzyl 1,1 O-phenanthroline were tested on two human cells cancer, myeloma (NS-1) and HeLa cells line. Cytotoxic activity was evaluated by trypan blue exclusion assay and their activity was expressed by the concentration inhibiting 50% of the cell growth (lCso)' The ICso of each compound was determined by probit analysis.Results: The results showed that the ICso values of 1,1 O-phenanthroline derivatives ranged from 4.68 to 15.63 pM on myeloma cell and from 2.82 to 16.89 pM on HeLa cell. The 4-Bromo-3(2-bromoetyll-2metyl-1 , 1 O-phenantroline (3) and (1 )-N-(4-benzyloxy-3-methoxy-benzyll-1 ,1 O-phenanthrolinium chloride (6) with ICso values ranged from 4.68 to 4.72 pM on myeloma cell showed the same (p>0.05) cytotoxicity with doxorubicin, with ICso values ranged from 2.82 to 3.08 pM on HeLa cell showed the higher (p

Non-ST elevation of acute myocardial infarction caused by probable acute stent thrombosis after drug eluting stent implantation: a case report Budi Yuli Setianto Bagus Andi Pramono
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 44, No 01 (2012)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

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Nowadays, one of the modalities in performing reperfusion in coronary artery disease ispercutaneous coronary intervention. Before drug eluting stent era, the problem that occurs themost among interventional cardiologists is restenosis. After drug eluting stent era, restenosiscan be reduced significantly. Unfortunately, it further develops another problem, that is calledstent thrombosis. Mechanism of stent thrombosis is related to vascular response, thrombosis,coagulation, and clinical factors. Based on our experience, non-ST elevation acute myocardialinfarction is caused by probable acute stent thrombosis after drug eluting stent implantation.Therefore, the prevention and therapeutic approaches should be put first in our priority becauseit can provide a serious complication.

The effectiveness of rivanol tampon" compared with burowi tampon in acute diffuse otitis externa (ADOE)patients I Wayan Marthana Kedel, Edhie Samodra Bambang Udji Djoko Rianto
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 41, No 03 (2009)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

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Background: Acute diffuse otitis externa (ADOE) is one of the common diseases in ENT Department, Dr. Sardjito Hospital, with the frequency of 9-1 2%. The most common microorganism found is Pseudomonas aeruginosa. Infection usually occurrs after taking a bath, washing hair, and scratching the external ear canal. Treatment of acute diffuse otitis externa which consists of topical preparations, Le. antibiotics and antiinflammation tampon or ear drop, is relatively expensive. A rationale, high recovery rate, cheap, and easily found preparation is needed. Burowi solution is one of the recommended treatment, but it is less popular. Objective: The goal of this study was to know the effectiveness of Rivanol" tampon compared with Burowi tampon in acute diffuse otitis externa. Methods: A randomized single-blind controlled trial (RCT)was performed at the outpatient clinicof ENTDepartment of DrSardjito General Hospital, Yogyakarta. Sixty-five subjects who were'diagnosed as ADOEwho met inclusion criteria were randomly allocated into two groups, that is, Rivanol"group and Burowi group. The ear canal was cleaned, and then Rivanol"tampon or Burowi tampon was applied. Evaluation was performed on day 3 and 5. Primary outcome was the recovery rate, and secondary outcomes were adverse reaction and marginal costeffectiveness. Results: Of 65 subjects, 33 were alloc'8ted into Rivanol"group and 32 were. allocated into Burowi group. The recovery rate in Rivanol"group was 75.4%, while in Burowigroups was 49.3%. The difference in recovery rate between the two groups was statistically significant (p: 0.018; RR4.350; 95% CI1.225-15.442). Adverse reaction in Rivanol"group was dampness and soggy feeling in the ear canal (1.5%), while in the Burowigroup the adverse events were pain and burnt sensation inthe ear canal (3.1%), but they could be tolerated and disappeared on day 5. Analysis on marginal cost-effectiveness showed that the cost to cure one acute diffuse otitis externa patient with Rivanol"tamponwas Rp 1,000. Conclusion: Rivanol"tampon was more clinicallyeffective and more cost-effective than Burowitampon for acute diffuse otitis externa treatment. Key words: acute diffuse otitis externa, Rivanol(!),Burowi,.clinical and cost effectiveness

Premenstrual syndrome (PMS) and premenstrual dysphoric disorder (PMDD) in Indonesian women Ova Emilia Ova Emilia Ova Emilia
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 40, No 03 (2008)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

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Background: Data from epidemiologic surveys showed that the burden of illness caused by premenstrual syndrome could not be underestimated. It has been estimated that the frequency of premenstrual symptoms was quite high (80-90%1, and in some women, the symptoms were so severe that they might interfere with daily activities. Most epidemiological studies of PMS were conducted in Western women, though a few small sample-size studies of Chinese and Japanese women indicated that they were affected by PMS. Objective: To find out the characteristic features of premenstrual symptoms in Indonesian women.Methods: Four hundred and sixty women aged between 20 and 49 yrs. with regular menstrual cycles, who were seen at a Women Health Clinic, were assessed regarding their premenstrual symptoms using the Premenstrual Symptoms Screening Tool (PSSTI. They were able to provide informed consent. The study was conducted in July 2007 until January 2008.Results: As many as 95% of these women were found to have at least premenstrual symptoms. The rates of prevalence of moderate to severe PMS and PMDD in this study were 3.9% and 1.1 %, respectively, which were lower than those in Western or Chinese and Japanese women. Only 5 women with moderate to severe PMS and PMDD were treated and 10.5% women seek herbal medicine to reduce premenstrual symptoms.Conclusion: Severe to moderate premenstrual symptoms and PMDD were suffered by 5% women and mostly affected 20-29 year old women. Medical treatment was rarely sought, but herbal medicine was often used to relieve premenstrual symptoms.Key words: PMS - PMDD - Indonesian women

Relation between vitamin C and vitamin E levels with malondialdehyde in elderly Prasetyastuti Sunarti, Dyah Arum Retnaningstyas Irianti Puji Lestari
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 39, No 03 (2007)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

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Background: Aging is a natural process during life. One of the aging theories is related to free radical production. Increase of free radical production can trigger lipid peroxidation that result in peroxide lipid such as malondialdehyde (MDA). The lipid peroxidation can be inhibited by body defence system such 8S vitamin C and vitamin E.ObJective: to understand the relation between plasma vitamin C, vitamin E and MDA levels in elderly Method: This was an observational research with cross sectional design. The subjects were thirty two elderly who live in Pakem district, Sleman. Determination of vitamin C and peroxide lipid level used spectrophotometer, whereas vitamin E level was determined by spectrofluorometer.Reaulta : The concentration of vitamin C , vitamin E and MDA of the subjects were 0.50 + 0.26 mglml, 26.38 ..:!:. 7.95 pg/ml and 0.23 ..:!:. 0.13 nmol/ml respectively. The correlation of both vitamin C vs MDA and vitamin E vs MDA were not significant, r = -0.012 and -0,108, respectively.Conclualon: There were no correlation between vitamin C, vitamin E and MDA levels.Keyword: elderly ; free radical;vitamin C; vitamin E; malondialdehyde.

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Nurhadiyahya

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nurhadiyahya@ugm.ac.id

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+6289672800034

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Kab. sleman,

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INDONESIA