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El-Hayah : Jurnal Biologi
Published by Universitas Islam Negeri Maulana Malik Ibrahim Malang
ISSN : 20860064     EISSN : 26570726     DOI : https://doi.org/10.18860/elha
Core Subject : Health, Science, Agriculture, Social,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Immunology & microbiology Medicine & Pharmacology
El-Hayah : Jurnal Biologi, publishes peer-reviewed research and review articles of special importance and broad interest in any area of biology (such as botany, zoology, ecology, microbiology, physiology, nanobiology, marine biology, hydrobiology, neurobiology, genetics, developmental biology, biochemistry, molecular biology, biophysics, and biomedical sciences), as well as covers the special topic on the relationship between science and religion.
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Pertanian dan Biologi (Lain-Lain)
Articles 178 Documents
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Conservation Of Juwet (Syzygium Cumini) Plant Using In Vitro Culture Techniques Ummah, Maslahatul; Mashluhah, Kholidatul; Resmisari, Ruri Siti
El-Hayah : Jurnal Biologi Vol 7, No 3 (2019): EL-HAYAH (VOL 7, NO 3 SEPTEMBER 2019)
Publisher : Department of Biology Science and Technology Faculty UIN Maulana Malik Ibrahim Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18860/elha.v7i3.10056

Abstract

Juwet (Syzygium cumini)  is a plant that has benefits as a medicine for type II diabetes mellitus, lungs, coughing, laxative urine. However, juwet is a scarce plant, it needs cultivation as a form of cuonservation. One of them is by utilizing biotechnology, which is of plant tissue culture. This study aims to determine the effect of the combination of 2,4-D and BAP growth regulators on the growth of juwet embryonic callus and the effect of the combination NAA and BAP on the growth of axillary bud juwet as a conservation effort. This research is experimental. Using a Completely Randomized Design (RAL) with combination 2,4-D (0; 0.5; 1; 1.5; 2; 2.5; 5) mg/L, and BAP (0; 0.25; 0.5; 0.75; 1) mg/L and combination NAA (0; 0.25; 0.5; 0.75; 1) mg/L and BAP (0; 0.5; 1; 1.5; 2) mg/L. Analysis by Two Way ANAVA test α = 5%. If there is a significant difference, the Duncan Multiple Range Test (DMRT) test with a significant level of 5%. Addition of 2.5 mg/L and 3 mg/L 2.4-D without BAP can induce intermediate callus, brownish yellow and there is a large cell nucleus in each cell. While the results of axillary bud growth is treatment in BAP 1 mg/L without NAA is the most effective interaction on the emergence of buds is 26.6 days after planting with the total of buds as much as 6.66, and the highest buds 5.37 cm and the highest total of leaves, namely 8.33 strands. 
PENGARUH LOGAM BERAT Cd, Pb TERHADAP PERUBAHAN WARNA BATANG DAN DAUN SAYURAN Widowati, Hening
El-Hayah : Jurnal Biologi Vol 1, No 4 (2011): EL-HAYAH (VOL 1, NO 4, Maret 2011)
Publisher : Department of Biology Science and Technology Faculty UIN Maulana Malik Ibrahim Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18860/elha.v1i4.1786

Abstract

Cd, Pb heavy metal absorption in aquatic vegetables: Genjer (Limnocharis flava), Kangkung air (Ipomoea aquatica Forsk) dan Selada air (Nasturtium officinale R. Br), studied to see the relationship with green figure scale. The research objective was to identify the accumulated Cd, Pb in the consumed organ vegetable, and their impacts to the levels of green figure scale. The research was undertaken by planting three kinds of the aquatic vegetables to the contaminated pure Cd,pure Pb media, mixture of Cd and Pb with factorial randomized block design. Data were analyzed by factorial One-Way Anova and further test of Duncan and HSD to see the difference of Cd, Pb and green figure scale, regression and correlation to know the contribution of Cd, Pb in influencing the green figure scale. Variety of vegetables, media, organ, and the interaction influences the accumulation of Cd, Pb and level of green figure scale. There is a relationship between Cd, Pb metal accumulation to the level of  green figure scale of the aquatic vegetables, with negative correlation that Cd, Pb decreased the vegetable green figure scale.Keywords: the influence of Cd, Pb; aquatic vegetables; green figure scale
KAJIAN KOMPOSISI SERANGGA POLINATOR TANAMAN APEL (Malus sylvestris Mill) DI DESA PONCOKUSUMO KABUPATEN MALANG Apituley, Frank Leonardo; Leksono, Amin Setyo; Yanuwiadi, Bagyo
El-Hayah : Jurnal Biologi Vol 2, No 2 (2012): EL-Hayah (Vol 2, No 2, Maret 2012)
Publisher : Department of Biology Science and Technology Faculty UIN Maulana Malik Ibrahim Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18860/elha.v2i2.2213

Abstract

Pohon apel merupakan salah satu jenis pohon yang tidak dapat melakukan penyerbukan sendiri. Bunga apel tergantung pada serangga penyerbuk atau serangga polinator. Penelitian ini bertujuan untuk mengetahui komposisi serangga kanopi yang berpotensi sebagai polinator bunga apel dan menganalisis pola kunjungannya. Pengamatan serangga dilakukan secara visual. Pengukuran faktor lingkungan (suhu, kelembaban, dan cahaya). Analisis data struktur komunitas serangga pada saat musim berbunga dan berbuah didapatkan dari nilai penting dan diversitas (Indeks Shannon Wienner).  Pola kunjungan dan komposisi serangga polinator dilakukan dengan membandingkan saat musim berbunga dan musim berbuah. Parameter yang dibandingkan diversitas, kelimpahan dan komposisi. Diversitas dan  dan  kelimpahan dibandingkan dengan uji  anova, sedangkan komposisi dibandingkan dengan IBC (Indeks Bray Curtis). Pola kunjungan harian serangga polinator bunga apel dianalisis dengan membandingkan rata-rata kunjungan pada periode I, II, III, IV, dan V. Serangga polinator yang dikoleksi pada perkebunan apel musim bunga lebih tinggi yakni (363 individu) dari pada musim buah (151 individu). Nilai indeks keanekaragaman serangga polinator pada saat musim berbunga lebih tinggi (2,08) dibandingkan musim buah (1,27). Kelimpahan serangga polinator antara musim bunga dan buah signifikan P 0,001. Kesamaan serangga polinator antara musim bunga dan buah denga indeks Bray Curtis sebesar 0,76 untuk musim bunga (AC, yaitu jam 07.00-08.15 dengan 12.00-13.15) dan 0,74 untuk musim buah (AB, yaitu jam 07.00-08.15 dengan 09.00-10.15). Analisis faktor lingkungan suhu, kelembaban, dan intensitas cahaya terhadap kelimpahan serangga polinator didapatkan korelasi yang positif dengan nilai R-square yakni 43,2%.  
PRODUKSI BAKTERIOSIN OLEH Lactobacillus plantarum DJ3 DAN APLIKASINYA SEBAGAI PENGAWET DAGING Hariani, Liliek
El-Hayah : Jurnal Biologi Vol 4, No 1 (2013): EL-HAYAH (VOL 4, NO 1, September 2013)
Publisher : Department of Biology Science and Technology Faculty UIN Maulana Malik Ibrahim Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18860/elha.v4i1.2617

Abstract

Lactic acid bacteria (LAB) are able to inhibit other bacteria by producing protein, named as bacteriocin. Bacteriocin which  produced by LAB is useful to inhibit pathogenic bacteria that harmful to human health or even makes food spoil. Bacteriocin is effective as antibacterial agent against pathogenic bacteria. Crude Extract of bacteriocin that produced by Lactobacillus plantarum DJ3 is able to inhibits the growth of E. Coli (4 mm) and S. aureus (5.33 mm). Application of bacteriocin in beef show that it able to inhibita the growth of bacteria. The amount of bacteria in beef that stored in 8 hours with bacteriocin addition are 1,3 X 108 CFU/g, and 3.7 X  108 CFU/g without bacteriocin addition. While the amount of bacteria in beef that stored in 12 hours with bacteriocin addition are 2.0 x 109 CFU/g and 1.5 x 1011 without bacteriocin addition
MEKANISME TERJADINYA INFLAMASI DAN STRES OKSIDATIF PADA OBESITAS rahmawati, ana
El-Hayah : Jurnal Biologi Vol 5, No 1 (2014): EL-HAYAH (VOL 5, NO 1, September 2014)
Publisher : Department of Biology Science and Technology Faculty UIN Maulana Malik Ibrahim Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18860/elha.v5i1.3034

Abstract

Obesity is a predisposing factor for metabolic syndrome that increases the risk of development of chronic diseases such as type 2 Diabetes Mellitus and atherosclerosis. Obesity is associated with a chronic inflammatory condition with progressive infiltration of immune cells in the adipose tissue of obesity. Adipose tissue is not only a triglyceride storage organ, but studies have shown the role of white adipose tissue as a producer of adipokines. Besides adipokines, also found several inflammatory mediators, such as interleukin-6 (IL-6). Adipokines have the function of regulating food intake, thus providing a direct effect on weight control. It is played by leptin, which is working on the limbic system by stimulating the uptake of dopamine, creating a feeling of fullness. But these adipokines induces production of reactive oxygen species (ROS), resulting in a process known as oxidative stress (OS). There are several mechanisms in producing the oxidative stress . The first is the oxidation of mitochondrial and peroxisomal fatty acid oxidation, which can generate ROS in the oxidation reaction, whereas other mechanisms is over-consumption of oxygen, which generates free radicals in the mitochondrial respiratory chain were found together with oxidative phosphorylation in the mitochondria. High fat diet also capable of generating ROS because it can alter the oxygen metabolism
Antimicrobial Activity and Identification The Compounds of Methanol Extract from The Pleurotus Ostreatus Fruiting Body Prastiyanto, Muhammad Evy; darmwati, Sri; iswara, Arya; Setyaningtyas, Agnis; Trisnawati, Laily; Syafira, Anna
El-Hayah : Jurnal Biologi Vol 6, No 1 (2016): EL-HAYAH (VOL 6, NO 1,September 2016)
Publisher : Department of Biology Science and Technology Faculty UIN Maulana Malik Ibrahim Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18860/elha.v6i1.4082

Abstract

Pleurotus ostreatus is an edible mushroom that also has potential as medicinal values. In this study, fruiting body of P. ostreatus was tested for its ability to inhibit the growth of fungi and bacteria. The fruiting body powder of P. ostreatus was extracted using methanol by maceration method. Analysis of this compound was done by using anisaldehid sulfuric acid, Dragendorff reagent, and FeCl3. Using the agar well diffusion technique, the extracts were tested for their antimicrobial activity against Staphylococcus aureus (Gram positive), Enterobacter aerogenes (Gram negative) and Candida albican (yeast). The spot results on TLC using crude extract of P. ostreatus is terpenoids. Zone of inhibition for the various extracts varied between 10.9 - 23.2 mm. Ten miligrams extract exhibit maximum antimicrobial activity against most of the tested pathogens 
Phytoplankton Community at Intensive Cultivation System of Whiteleg Shrimp, Litopenaeus vannamei in Probolinggo, East Java Arifin, Nasrullah Bai; Fakhri, Muhammad; Yuniarti, Ating; Hariati, Anik Martinah
El-Hayah : Jurnal Biologi Vol 6, No 3 (2017): EL-HAYAH (VOL 6, NO 3,September 2017)
Publisher : Department of Biology Science and Technology Faculty UIN Maulana Malik Ibrahim Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18860/elha.v6i3.4800

Abstract

Phytoplankton is a source of natural feed for shrimp cultivation in the pond. Phytoplankton productivity increases by the increasing nutrient content in the pond. Feed and metabolic waste is the sources of nutrient for phytoplankton growth. This study aimed to evaluate productivity and identify phytoplankton at intensive whiteleg shrimp, Litopenaeus vannamei cultivation system. This study was conducted at three intensive whiteleg shrimp located in Probolinggo, East Java. Samples were collected on the early and the late of culture period (day 17 and 87 after stocking). Four environmental parameters including total of ammonia nitrogen (TAN), nitrate, orthophosphate, total of suspended solid (TSS), and chlorophyll-a were measured. Identification and density of phytoplankton were also performed of each pond. The result showed that productivity of three ponds was 22,893.83 kg/ha to 23,600.61 kg/ha with an average size 12.74 g to 14.35 g. During culture period, the concentration of TAN, TSS and chlorophyll-a tended to increase. Meanwhile, the average of both nitrate and orthophosphate tended to decline. Several phytoplankton identified in this study were in the genus of Oocystis, Chlorella, Nannochloropsis, Chaetoceros, Stephanodiscus, Nitzschia, Coscinodiscus, Cyclotella and Ulothrix. Phytoplankton of the group Chlorophyta is predominance for pond 1 and 2, while pond 3 was dominated by phytoplankton in the group of Diatom/Baccillariophyta. This study indicated that the present of phytoplankton in the pond provide natural feed and good environmental condition for shrimp cultivation.
ANTIBACTERIAL ACTIVITY ETHYL ACETATE EXTRACTS OF EARTHWORMS (Lumbricus rubellus, Eisenia foetida, Nereis sp) TOWARD Staphylococcus aureus, Enterococcus faecalis, Salmonella thyposa INVITRO Kartikaningsih, Hartati; Maharani, Sarastria; Sartika, Fitarina
El-Hayah : Jurnal Biologi Vol 7, No 2 (2019): EL-HAYAH (VOL 7, NO 2 Maret 2019)
Publisher : Department of Biology Science and Technology Faculty UIN Maulana Malik Ibrahim Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18860/elha.v7i2.8248

Abstract

Earthworms had a mechanism of antibacterial. The research aimed to observe Lumbricus rubellus, Eisenia foetida, Nereis sp. antibacterial activity against Salmonella thyposa, Staphylococcus aureus, Enterococcus faecalis in vitro compared to ampicillin antibiotics. All the worms extracted using ethyl acetate extraction and tested their MIC. The compound of amino acids of the worms was analyzed by HPLC and nanodrop.  Lumbricus rubellus was the best anti-bacteria activity followed by Eisenia foetida and Nereis sp., but these activities less than ampicillin antibiotic. Observations with SEM showed these worms extract caused cell leakage in all of these bacteria. Protein content with Nanodrop testing revealed the highest protein content was Lumbricus rubellus (21.75 ppm) followed by Eisenia foetida (21.32 ppm) and Nereis sp. (20.98 ppm), as well as for amino acids levels, there were Lumbricus rubellus (24.66%), Eisenia foetida (22.78%), Nereis sp. (18.37%). From the 15 amino acids detected, all of the worms had the same sequence of fourth the highest amino acids (Glutamate, Aspartate, Leucine, Arginine) and fourth the lowest amino acid levels (Methionine, Hystidin, Tyrosin, Glisan). It had not been tested amino acid sequences of antibacterial compounds of these worms (Lumbricin 1: Phe-Ser-Lys-Tyr-Glu-Arg in Lumbricus rubellus worms, Fetidin 1: Ala-Met-Val-Ser-Ser and Fetidin 2: Ala-Met- Val-Gly-Thr in the Eisenia foetida worm, Hemerythrin: His-Glu-Asp in Nereis sp).
Identification of Phytochemicals Compound On the Ethanol Extract ‎of Banana Peel Musa Balbisiana Colla “Kepok Putih” and Musa X ‎Corniculata Lour. “Tanduk”‎ Wardati, Fauchil; Fitriahani, Feby; Laily, Ainun ‎ Nikmati
El-Hayah : Jurnal Biologi Vol 8, No 1 (2020): EL-HAYAH (VOL 8, NO 1 SEPTEMBER 2020)
Publisher : Department of Biology Science and Technology Faculty UIN Maulana Malik Ibrahim Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18860/elha.v8i1.11318

Abstract

Indonesia is the largest banana producer in Asia and each year continues to increase its production. The number of banana production in Indonesia reached 4,177,155 tons in 2003 and increased continuously to 6,373,533 tons in 2009. The abundance of banana peel is not comparable with the utilization of a banana peel in the society which has so far not been processed much apart from being animal feed. Two types of bananas that are often processed but the peels have not been optimally utilized yet are “kepok putih” and “tanduk”. Banana peel of “kepok kuning” extract contains alkaloids, flavonoids, quinones, saponins and tannins. However, the phytochemical compounds of banana peel “kepok putih” and “tanduk” have not been widely studied. Thus, this research aims to identify the phytochemical compounds from banana peel of “kepok putih” and “tanduk”. The extraction method which used in this experiment was the maceration method using 96 % ethanol solvent. Based on the test results, which may be obtained, it is known that the ethanol extract of banana peel “kepok putih” contains flavonoids, tannins,  alkaloids, while “tanduk” contains flavonoids, saponins, tannins, and alkaloids
OPTIMASI KONSENTRASI INOKULUM, RASIO C:N:P DAN pH PADA PROSES BIOREMEDIASI LIMBAH PENGILANGAN MINYAK BUMI MENGGUNAKAN KULTUR CAMPURAN Zam, Syukria Ikhsan
El-Hayah : Jurnal Biologi Vol 1, No 2 (2010): EL-HAYAH (VOL 1, NO 2, Maret 2010)
Publisher : Department of Biology Science and Technology Faculty UIN Maulana Malik Ibrahim Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18860/elha.v1i2.1691

Abstract

The purposes of this research were to obtain the best inoculum concentration, C:N:P ratio, and pH, and also to identify the ability of mixed culture of hydrocarbonoclastic bacteria in  oil waste degradation. The isolats were used are Acinetobacter baumanni, Alcaligenes eutrophus, Bacillus sp1., Methylococcus capsulatus, Bacillus sp2., Morococcus sp., Pseudomonas diminuta, Xanthomonas albilineans, Bacillus cereus and Flavobacterium branchiophiia. Variation of inoculum concentrations were 10%, 15%, and 20% (v/v), C:N:P ratios were 100:10:1, 100:10:0,5, 100:5:1, and 100:5:0,5, and pH were 6,5, 7,0, 7,5. Observed parameters in optimization were Total Plate Count (TPC) the culture every 24 hours, Total Petroleum Hydrocarbon (TPH) and Chemical Oxygen Demand (COD) examined at the end of the bioremediation period. Best optimization result then analyzed with GC/MS. Optimization result indicated the best inoculum concentration was 10% with TPH degradation 61,79% and COD slope 61,75%. It is assumed that the low value of TPH degradation and COD slope at 15% and 20% inoculum concentration were caused by competition inside the bacterial population at that high inoculum concentration. The competition result in low growth and degradation. C:N:P ratio was 100:5:1 with TPH degradation 66,55% and COD slope 85,18%. It is assumed that the C:N:P ratio is equal, so it can enhance the bioremediation procces. The best pH was 7,5 with TPH degradation 73,24% and COD slope 86,28%. The process at the optimum conditions using inoculum as a mixed culture enhanced the bioremediation process with the result as follows, TPH degradation 93,06%, COD 90,73% for treatment. The chromatogram indicated that total hydrocarbon compound from nC9 – nC32 have been degraded by 43,413% – 63,117%. A good result of bioremediation was obtained from mixed culture inoculum at 10% concentration, C:N:P ratio of 100:5:1, and pH 7,5.Keywords:    Bioremediation, hydrocarbonoclastic bacteria, inoculum concentration, C:N:P ratio, pH

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