El-Hayah : Jurnal Biologi
El-Hayah : Jurnal Biologi, publishes peer-reviewed research and review articles of special importance and broad interest in any area of biology (such as botany, zoology, ecology, microbiology, physiology, nanobiology, marine biology, hydrobiology, neurobiology, genetics, developmental biology, biochemistry, molecular biology, biophysics, and biomedical sciences), as well as covers the special topic on the relationship between science and religion.
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IDENTIFIKASI SENYAWA ANTIDIABETES SECARA in Silico PADA Carica pubescens Lenne & K. Koch
Ainun Nikmati Laily;
Ahmad Nuruddin Khoiri
El-Hayah Vol 5, No 4 (2016): EL-HAYAH (VOL 5, NO 4,Maret 2016)
Publisher : Program Study of Biology, Science and Technology Faculty, UIN Maulana Malik Ibrahim Malang
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DOI: 10.18860/elha.v5i4.3469
Carica pubescens Lenne K. Koch is one of the typical highland crops in Indonesia. This plant can be found in the region of Cangar and Bromo in East Java, as well as the Dieng Plateau, Central Java Wonosobo-Banjarnegara. Morphological, chemical content, and analysis of protein banding pattern of the Carica pubescens has been done, but more about the use of active compounds for pharmaceutical raw materials, in Diabetes Mellitus type 2 have not been studied. One therapeutic approach to lowering blood sugar levels posprandial is to slow down or inhibit the absorption of glucose through inhibition of the carbohydrates hidrolitic enzyme such as α-amylase and α-glucosidase. This study aims to identify antidiabetic compounds in silico. Antidiabetic compounds were analyzed by methods of docking. The results showed that papain and cysteine protease in C. pubescens have better binding affinity to α-amylase and α-glucosidase compared to iridoid glycosides. Papain and cysteine proteases isolated from C. pubescens are potential to be developed as an antidiabetic.
Diversity of Echinoderms in Balekambang Beach, Malang District, East Java
Buwono, Nanik Retno;
Fitri, Nur Lailatul
El-Hayah : Jurnal Biologi Vol 6, No 2 (2017): EL-HAYAH (VOL 6, NO 2,March 2017)
Publisher : Department of Biology Science and Technology Faculty UIN Maulana Malik Ibrahim Malang
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DOI: 10.18860/elha.v6i2.4532
Echinoderms is a component of biodiversity in the coral reefs. Echinoderms play an important role in ecosystem of coral reefs especially in food web, generally as predators and detritivors. This report aims to understand composition and density relative of fauna echinoderms on the Balekambang beach. The Transect line method were used and data retrieval was conducted at three stations. The result showed that echinoderms found in Balekambang beach consists of 7 species that are included in three classes, they were Phyllachantus imperialis, Tripneustes gratilla, Diadema setosum, Clypeaster reticulatus (Class Echinoidea), Archaster typicus (Class Asteroidea), Ophiotrix fragilis and Macrophiothrix belii (Class Ophiuroidea). The highest abundance of echinoderms on the station where located in the mangrove area which was dominated by Archaster typicus. The diversity of echinoderms on Balekambang beach were moderate category. The observations of chemical and physics parameters of the aquatic environment and the substrate obtained quite optimal for life of Echinoderms
Isolation and Identification of Cellulose Degrading Bacteria from Banana Peel Compost
P., Yendania Grevitara;
F., Badriyatur Rahma;
P., Hellen Septirangga;
Y., Irma Dahlia;
Suarsini, Endang
El-Hayah : Jurnal Biologi Vol 7, No 1 (2018): EL-HAYAH (VOL 7, NO 1 September 2018)
Publisher : Department of Biology Science and Technology Faculty UIN Maulana Malik Ibrahim Malang
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DOI: 10.18860/elha.v7i1.7241
Cellulolytic bacteria are bacteria that have the ability to hydrolyze cellulose complexes into smaller oligosaccharides and eventually become glucose. Glucose is used as a carbon and energy source for bacterial growth. This study was conducted to isolate the cellulose degrading bacteria from banana peel compost that produce cellulose enzymes based on the clear zone that visible around the colony. The cellulolytic activity was determined by the ability of bacteria to hydrolyze the Carboxymethyl Cellulose (CMC) substrate. Determination of cellulolytic activity is known based on cellulolytic index calculation, the diameter total minus the diameter of the colony and divided by the diameter of the colony. The result of five bacterial isolates was found but only one bacterium had the potential to be a cellulose degradation. Based on the Microbact Gram-Negative Identification System, the bacterium is Burkholderia cepacia. These bacteria have an important role in nature as decomposers of various complex compounds, such as cellulose, hemicellulose, lignin, and pectin.
Fusarium Wilt Disease Control Using Biological Agents Trichoderma and Mycorrhizaeon Pepper
Baswarsiati, Diding Rachmawatiand
El-Hayah : Jurnal Biologi Vol 7, No 4 (2020): EL-HAYAH (VOL 7, NO 4 MARET 2020)
Publisher : Department of Biology Science and Technology Faculty UIN Maulana Malik Ibrahim Malang
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DOI: 10.18860/elha.v7i4.10661
Fusarium oxysporum is the main fungus disease that can wither plants, especially pepper. The fungus spread through diseased soil or already withered plants and then infect plants from its roots. Doing control by using antagonistic fungi such as Trichoderma sp. and Arbuscularmycorrhizae have been widely performed. Trichoderma sp. is a fungus rich with an antifungal activity that produce metabolites, both volatile and non-volatile. These metabolites produced by Trichoderma can diffuse through the dialysis membrane which capable to slow several pathogens growth. Mycorrhizae creates mutual symbiosis between certain types of fungi with roots, also known as biological agents, capable to control F. oxysporum on pepper and to help antibiotics formation. The study was conducted at Laboratorium Hama dan Penyakit BPTP East Java, starting from January to May 2016. This study used completely randomized design (CRD) with treatment consisted of 16 combined doses of Mycorrhizae and Trichoderma, each repeated 4 times that produce 64 test units. Mycorrhizae dose used is 0.0; 1.0; 2.0 and 4.0 grams per polybag, while the Trichoderma dose used is 0.0; 15.0; 30.0; and 45.0 grams per polybag. Data were statistically analyzed by variance analysis and followed by a BNT test of 0.05. The results showed Mycorrhizae 4 g /polybag and Trichoderma 45 g /polybag application could increase the incubation period of F. Oxysporum fungus, shorten xylem discoloration and then reduce wilted plants percentage. Mycorrhizae application can boost Trichoderma fungus in order to reduce wilt disease found in pepper plants
THE GENETIC POTENCY OF PLANTAIN AGUNG SEMERU VARIETY FROM LUMAJANG REGENCY EAST JAVA INDONESIA
Prahardini, P.E.R.
El-Hayah : Jurnal Biologi Vol 1, No 1 (2009): EL-HAYAH (VOL 1, NO 1,September 2009)
Publisher : Department of Biology Science and Technology Faculty UIN Maulana Malik Ibrahim Malang
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DOI: 10.18860/elha.v1i1.1684
Lumajang regency is one of the banana production centre in East Java having high- diversity of banana germ plasm. There are 33 cultivars of banana germ plasm in the regency,  consist of eaten ripe and plantain. One of uniqe plantain used as the symbol of Lumajang regency is plantain Agung Semeru variety, the local superior variety of this regency. This variety can grow well at 450 – 650 m above sea level. The uniqueness of banana Agung Semeru variety can be seen by the number of sucker per cluster (only  1 – 2 suckers per cluster), the size of the finger (33 – 36 cm long and 19 cm around) and the number of hand per bunch (only 1 – 2 hand per bunch). Other characteristics of the variety are the thickness of fruit skin, the long period of fruit storage (3 – 4 weeks after harvesting) and the sweetness of fruit flesh. Even though the skin changes from yellow to black, the flesh still can be consumed, because it doesn’t become soft. This variety also resistant to the Sigatoka disease compared to other plantain cultivars.
EVALUASI OOSIT KAMBING HASIL IVM SEBAGAI SALAH SATU FAKTOR PENENTU KEBERHASILAN DALAM AKTIVASI PARTENOGENESIS
Holil, Kholifah
El-Hayah : Jurnal Biologi Vol 2, No 1 (2011): EL-HAYAH (VOL 2, NO 1, September 2011)
Publisher : Department of Biology Science and Technology Faculty UIN Maulana Malik Ibrahim Malang
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DOI: 10.18860/elha.v2i1.1791
Aktivasi partenogenesis merupakan salah satu tehnik aktivasi oosit untuk menghasilkan embrio tanpa kontribusi dari sperma. Salah satu faktor yang menentukan keberhasilan tehnik ini adalah pada ketersediaan oosit yang berkualitas. Oleh karena itu penelitian ini bertujuan untuk mengevaluasi oosit kambing hasil IVM yang dapat digunakan untuk kepentingan dalam aktivasi partenogenesis tersebut.Sampel yang digunakan dalam penelitian ini adalah oosit yang diaspirasi dari folikel ovarium kambing yang diambil dari RPH Sukun Malang. Oosit di IVM selama 24 jam dan selama 27 jam dalam medium TCM-199 yang ditambah dengan fetal bovine serum (FBS), follicle-stimulating hormone (FSH) dan lutheinizing hormone (LH) dan diinkubasi pada suhu 38,5oC, 5% CO2. Pada jam ke 24 dan jam ke 27 setelah IVM dilakukan pengamatan yang meliputi ekspansi sel-sel kumulus dan keberadaan polar body I (PB-I) pada jam ke 30. Berdasarkan hasil penelitian menunjukkan bahwa oosit kambing yang di IVM sampai jam ke 24 belum menunjukkan adanya ekspansi sel-sel kumulus kualitas 2 dan penampakan PB-I. Hasil tersebut berbeda dengan oosit yang di IVM sampai jam ke 27. Pada pengamatan jam ke 27 ini menunjukkan bahwa terdapat 77,87% oosit yang sel-sel kumulusnya berekspansi (kualitas 2) dan PB-I yang nampak sebesar 95,32%. Dengan demikian maka dapat disimpulkan bahwa oosit kambing hasil IVM baru dapat digunakan untuk keperluan lebih lanjut khususnya untuk keperluan aktivasi partenogenesis pada jam ke 27.Kata kunci: evaluasi, aktivasi partenogenesis, oosit kambing hasil IVM
DAYA ANTIFUNGAL DEKOK KAYU MANIS (Cinnamomum burmanni) TERHADAP Candida Albicans SECARA IN VITRO
Rachma, Lailia Nur
El-Hayah : Jurnal Biologi Vol 3, No 1 (2012): EL-Hayah (Vol 3, No 1, September 2012)
Publisher : Department of Biology Science and Technology Faculty UIN Maulana Malik Ibrahim Malang
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DOI: 10.18860/elha.v3i1.2218
Candida  albicans  is  the  most  oportunis fungi  that  cause  flour  albus.  Cinnamomum burmanni have been widely known as therapy for flour albus. This efect caused by its chemical compound such as cinnamaldehyde, eugenol, cinnamic acid, limonene, cathecin, coumarin, linalool, and tannin. This research aims was to know the comparison of antifungal potency of Cinnamomum burmanni on Candida albicans in vitro. The design was true experimental. The decoction concentrations of Cinnamomum burmanni that were used were 8%, 4%, 2%, 1%, 0.5%, 0.25%, and 0.125% with three time repetition. The data was analized with One-way ANOVA test with convidence interval 95% (p 0.05). One-way ANOVA test gave result Cinnamomum burmanni had antifungal potency against Candida albicans. Minimum Inhibitory Concentration (MIC) of Cinnamomum burmanni was 1%. Minimum Fungicidal Concentration (MFC) of Cinnamomum burmanni was 2%. The conclusion was Cinnamomum burmanni had antifungal potency against Candida albicans in vitro.  Â
VAKSINASI LANSIA UPAYA PREVENTIF MENINGKATKAN IMUNITAS AKIBAT PROSES PENUAAN
Susanti, Nurlaili
El-Hayah : Jurnal Biologi Vol 4, No 2 (2014): EL-HAYAH (Vol 4, No 2, Maret 2014)
Publisher : Department of Biology Science and Technology Faculty UIN Maulana Malik Ibrahim Malang
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DOI: 10.18860/elha.v4i2.2629
Advancement in health sciences and technologies gives positive impact to improve life expectancy, but the other hand it can increases elderly population with all consequences. Old age is associated with high morbidity and mortality due to their susceptibility to suffer several diseases caused by lacking immunity, especially infectious diseases. Moreover, the occurrence of multiple morbidities, such as heart disease and vulnerability to stress, are the main confounding factors that may impact to immune functions of elderly. Aging affects both cellular and humoral responses of the innate and adaptive immune systems and causes decreasing the number of immune cells, their viability, functional capacity and their responses against pathogens that entering the body. Strategies for preventing these diseases would have a clear impact on improving healthy aging. Thus, vaccination strategies for elderly population are needed. Vaccines should be developed to take into consideration the peculiar age-induced variations of immune responsiveness
KARAKTERISASI KULTUR MAKROFAG HASIL ISOLASI Mouse Peritoneum Makrofag (MPM)
Susanti, Erna
El-Hayah : Jurnal Biologi Vol 5, No 3 (2015): EL-HAYAH (VOL 5, NO 3,September 2015)
Publisher : Department of Biology Science and Technology Faculty UIN Maulana Malik Ibrahim Malang
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DOI: 10.18860/elha.v5i3.3096
The aims of this study is to determine the MPM isolation techniques, results of the isolation and identification, so it can be used as a reference for the studies using macrophage cell cultures. The most appropriate source for culturing macrophages are the primary culture of MPM. Resident peritoneal macrophages (RPM) is a free-living phagocytic cells in the peritoneal cavity. Millions of resident macrophages can be taken from the mice. The peritoneal cavity is an easy place to harvest the resident macrophages. Identification of macrophages was conducted using flowcytometry with specific marker CD 11B. While the morphology of macrophages in culture was observed microscopically The results of the study showed the average number of macrophages 1.03 x 106 cells / ml were isolated from peritoneal cavity of mice. The result of identification with flowcytometry with marker CD 11 B shows positive results macrophages. While the observation of morphological characteristics of macrophages cultures show the same characteristics as the result of research conducted by Kim in 1997 and Wang et al, 2010 so that it can be concluded that macrophages can be isolated from the peritoneal cavity and can be used as a source of primary cultures of macrophages with good results and can be applied to studies related to the function and activation of macrophages in vitro. Keyword; Kultur Makrofag, Isolasi Mouse Peritoneum Makrofag (Mpm)
Effect of Node Position and Number of Stem Cutting on The Growth and Yield of ‘katuk’ (Sauropus androgynus (L.) Merr.)
Solikin, Solikin
El-Hayah : Jurnal Biologi Vol 6, No 4 (2018): EL-HAYAH (VOL 6, NO 4,March 2018)
Publisher : Department of Biology Science and Technology Faculty UIN Maulana Malik Ibrahim Malang
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DOI: 10.18860/elha.v6i4.6336
Katuk (Sauropus androgynus (L.) Merr.) has potential as a vagetable and medicinal plant. This study aimed to determine effect of node position and number of stem cuttings on the growth of katuk. The experiment  used Completely Randomized Design with two treatments arranged in split plots. The node position of stem cuttings as the main plot consists of top stem cutting (T, top – 8 nodes under shoot tip), middle stem cutting (M, 8 – 11 nodes under shoot tip) and bottom stem cutting (B, 16-20 nodes under shoot tip). The node number of stem cuttings as a subplot consists of 2 nodes (J1), 3 nodes (J2), 4 nodes (J3), and 5 nodes (J4). Each treatment combination was replicated three times.The results revealed that the top stem cutting resulted in the highest root, total plant dry weight and leaf area, as well stem cutting with 4 nodes produced the highest total plant dry weight and leaf area.