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Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati

biota
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Published by Universitas Atma Jaya Yogyakarta

ISSN : 25273221 EISSN : 2527323X DOI : doi.org/10.24002/biota

Core Subject : Science, Agriculture,

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati merupakan jurnal ilmiah yang memuat hasil-hasil penelitian, kajian-kajian pustaka dan berita-berita terbaru tentang ilmu dan teknologi kehayatian (biologi, bioteknologi dan bidang ilmu yang terkait). Biota terbit pertama kali bulan Juli 1995 dengan ISSN 0853-8670. Biota terbit tiga nomor dalam satu tahun (Februari, Juni, dan Oktober).

Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Pertanian dan Biologi (Lain-Lain)

Articles 1,193 Documents

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Keragaman Jenis Opisthobranchia di Taman Laut 17 Pulau Riung, Nusa Tenggara Timur Ngole, Maria M.; Oliva, Pantang K; Zahida, Felicia; Sidharta, Boy R.
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 10, No 1 (2005): February 2005
Publisher : Universitas Atma Jaya Yogyakarta

Penelitian inventarisasi jenis Opisthobranchia telah dilakukan untuk menunjang ekowisata di daerah Taman Laut 17 Pulau Riung. Taman laut ini terletak di kecamatan Riung, Kabupaten Ngada, Flores, Nusa Tenggara Timur. Kawasan ini di sebelah barat dibatasi oleh kabupaten Manggarai dan disebelah timur oleh desa Nggolonio, di utara dibatasi dengan laut Flores dan di selatan oleh Desa Wangka, Alo Mamek, dan Ten Terong. Areanya meliputi lima buah desa yaitu desa Lengkosambi, Tadho, Benteng Tengah, Nangamese dan Sambinasi. Secara geografis terletak antara 8o20â€™30â€ â€“ 8o28â€™30â€ LS hingga 90o55â€™30â€ â€“ 90o09â€™00â€ BT. Luas daratan sekitar 5 ha dan terumbu karang seluas 7.500 ha (Anonim a dan b. 1993). Opisthobranchia amat disukai oleh penyelam dan penggemar fotografi bawah laut dari mancanegara, namun masih amat sedikit penelitiannya di Indonesia

Pola Pergerakan Perilaku Agonistik Lobster Air Tawar Cherax quadricarinatus (Von Martens, 1868) Tarjono, Tarjono; Patria, Mufti P.
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 15, No 3 (2010): October 2010
Publisher : Universitas Atma Jaya Yogyakarta

This research on movement patterns of agonistic behavior in crayfish Cherax quadricarinatus was conducted at laboratory and used an aquarium size 25 cm x 10 cm x 15 cm. The patterns of agonistic behavior were recorded by using a CCD and digital camera. The recorded data were analyzed using The Adobe Premier computer program and using Jaccard similarity to solve the reciprocal movement patterns. Clustering based on the UPGMA (Unweighted Pair Group Method Average) indicated that the dissimilarity value was about 0,98 and the moving pattern of the agonistic behaviors were grouped into five groups, namely: 1) threatening; 2) arranging the strategy; 3) testing the enemies capability; 4) fighting; 5) avoiding and defending.

Profil Imunohistokimia Antioksidan Copper, Zinc-Superoxide Dismutase (Cu, Zn, -SOD) pada Ginjal Tikus Perinatal dan Neonatal Wresdiyati, Tutik; Adnyane, I Ketut Mudite; Prabandari, Silvia Arin; Sofiawati, Sofiawati
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 9, No 3 (2004): October 2004
Publisher : Universitas Atma Jaya Yogyakarta

The aim of the research was to observe the profile of intracellular antioxidant, especially copper, zinc-superoxide dismutase (Cu, Zn-SOD) in the kidney of perinatal and postnatal rats immunohistochemically. A total of twuelve Wistar rats were used for this research and they were divided into four groups that are 18 days (Fe18) and 20 days old fetus (Fe20), one day (N1) and three days neonatus (N3). There are three rats in each groups. Kidney from each group were taken and processed for routin paraffin embedding methods. The tissues sections were observed with Hematoxylin-eosin (HE) and immunohistochemical technique for Cu, Zn-SOD. Cu,Zn-SOD in the kidney were observed qualitatively in medulla, glomerulus dan cytoplasm of tubuli renalis proximalis and tubuli renalis distails, as well as quantitatively in the nucleus of tubuli renalis cells. The results showed that Cu, Zn-SOD increased in the kidney of perinatal rats, 20 days old fetus (Fe20), then it decreased in the tissues of the one days neonatus (N1), then were increased again in the kidney of three days neonatus (N3) group rats.

Perilaku Harian Buaya Muara (Crocodylus porosus, Schneider 1801) di Pusat Penyelamatan Satwa Jogja I., Purwo Setio; Muharromi, A. Fanani; Prihantono, Subekti; Qurniawan, Tony Febri; Nugraha, A. Prima; Eprilurahman, Rury
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 15, No 2 (2010): June 2010
Publisher : Universitas Atma Jaya Yogyakarta

The aim of this research was to find out the daily behaviour of Crocodylus porosus in Jogja Animal Rescue Center (PPSJ). The daily behaviour included feeding habit, social behaviour, basking and movement. This research was using Focal Animal Sampling Method and observed for four months. As individual targets were male and female dominant of C. porosus, Monti and Bunda. The Runs test showed that the behavior of C. porosus had a pattern in daily activities. Based on Mann-Whitney U test, both male and female of C. porosus did not have any difference in feeding habit (U=16; n1= 6; n2=6; p>0,05), social behaviour (U=29; n1= 8; n2=8; p>0,05), basking (U=12; n1= 5; n2=5; p>0,05) and movement (U=16; n1= 6; n2=6; p>0,05).

Kondisi Optimum untuk Produksi Kitinase dari Streptomyces Rkt5 dan Karakterisasi pH dan Suhu Enzim Yurnaliza, Yurnaliza; Margino, Sebastian; Sembiring, Langkah
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 13, No 3 (2008): October 2008
Publisher : Universitas Atma Jaya Yogyakarta

Chitinase is chitin degrading enzyme which is produced by Streptomyces Rkt 5 is isolated microorganism from peanut rhizosfer. This enzyme and its microorganism can be used in many agricultural, medicine and industrial purposes. The aim of the research was to find out the optimum condition for production of chitinase and to characterize of pH and temperature to chitinase activity. Optimalizing production the research had 4 treatments. The optimum conditions were achieved at mineral liquid medium containing with chitin 0,2% (w/v) as inducer, 10% (v/v) inoculum, pH 7 and 48 hours incubation. The crude enzyme was partially purified by salting out with 70% ammonium sulfate resulted in 3.31 time more purity enzyme than the crude one. This enzyme had maximum activity at 50oC and pH 5.5.

Studi Kepiting Mangrove di Delta Mahakam, Kalimantan Timur Pratiwi, Rianta
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 12, No 2 (2007): June 2007
Publisher : Universitas Atma Jaya Yogyakarta

The research was carried out in July 2004 at Makaham Delta, East Kalimantan. Thisresearch observed the diversity and the distribution pattern of mangrove crabs atmuara Ilu, Kaely, Bayor, and Beji. There are 33 species consisting of 7 families and 6908individu of crabs found in this research. Most of the mangroves crabs are clumpdistributed. The research method by using frame transects 1 m2 x 1 m2 with 5 up to 10meters distance (based on the thickness of mangrove in those areas). The distributionpattern of crabs analyze by using Index Morisita according to Brower and Zar.

Purifikasi dan Karakterisasi Krom Reduktase Bacillus sp LKA9 Ali, Alimuddin; Soetarto, Endang Sutariningsih; Widada, J. Sri
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 11, No 2 (2006): June 2006
Publisher : Universitas Atma Jaya Yogyakarta

Chromate reductase is one of the potential enzymes for hexavalent chrom detoxification. Most of the enzyme is produced by bacteria, especially Bacillus. The aim of this research was to study chromate reductase activity isolated from Bacillus sp LKA9. Bacillus sp LKA9 was isolated from leather tannery liquid waste and used as a model in the experiment. Bacillus sp LKA9 was isolated through enrichment culture using Salt Base Solution containing 3 mM K2CrO4. Chromate reductase was isolated from bacteria by growing on a liquid medium containing chrom hexavalen (Cr VI) through several steps. The first step of the isolation process was to use the precipitated process using ammonium sulphate (30-80%). The next step, crude enzymes from the first step was partially purified through DEAE-Cellulose of Ion Exchange Chromatography Column. Diphenylcarbazide methods was used to examine the activity of enzyme fractions. The result of the experiment revealed that all protein could be precipitated by ammonium sulphate, and the cytoplasmic fraction at saturation of 50-70% showed high enzyme activity. Purified enzymes showed an increase activity 69,385 times to that of crude enzymes. The enzyme optimal had temperature and pH were 350C and 5; respectively. KM of enzyme was 0,0075 mM, and Vmax was 2500 ïmol/minute/mg protein. Enzyme activity was not inhibited by Cu2+, but an ion Ag2+ and Hg2+ inhibited the enzyme activity un-competitive. The activity of enzyme was demonstrated on SDS-PAGE by appearing typically band with molecular weight 29,26 kDa, it was assumed as chromate reductase.

Analisis Gen 12SrRNA Dari DNA Mitochondria Kelelawar Pemakan Buah Chironax Melanocephalus (Chiroptera: Pteropodidae) Di Taman Nasional Gunung Halimun Zein, M. Syamsul Arifin; Maharadatunkamsi, Maharadatunkamsi
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 8, No 1 (2003): February 2003
Publisher : Universitas Atma Jaya Yogyakarta

A study on genetic variation of the high mountain fruit bat (Chironax melanocephalus) was conducted in Gunung Halimun National Park. DNA total from liver tissues were extracted and fragment of the 12SrRNA gene region of mitochondrial DNA were amplified by Polymerase Chain Reaction (PCR). Nucleotide sequence of the PCR products were determined by automated sequencer. Seven haplotype were found among 20 individuals from 6 localities, namely: South Halimun Mountain, Kendeng Mountain, Botol Mountain, Pasir Cangkuang Mountain, Kencana Mountain, and Buligir Putih Mountain. Haplotype diversity and nucleotide diversity were 0.76 and 0.0222. DNA distances values ranged from 0.0028 to 0.0202.

Variasi Biokimia Genetik Populasi Ikan Betutu (Oxyeleotris marmorata, BLKr.) di Waduk Penjalin Brebes Susanto, Agus Hery; Suryaningsih, Suhestri
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 11, No 3 (2006): October 2006
Publisher : Universitas Atma Jaya Yogyakarta

Penjalin water reservoir in Brebes Regency, Central Java, is one of the habitats of the sand goby. A study on its genetic diversity using approaches of isozyme analysis was needed to support domestication of the fish in this area. This study was aimed at the biochemical-genetic variation of sand goby population in Penjalin water reservoir based on esterase (EST), peroxidase (PER), malate dehydrogenase (MDH), aspartate amino-transferase (AAT), and acid phosphatase (ACP) polymorphisms. Visualization of the isozymes was carried out employing horizontal electrophoretic technique with potato starch gel and buffer system of L-histidin monohydrate. Of the five isozymes, ACP was not well-visualized in all samples tested while the remaining four showed no polymorphisms. It could be concluded that there is no biochemical-genetic variation of sand goby population in Penjalin water reservoir based on isozymes of EST, PER, MDH, and AAT.

Dominansi dan Seleksi Jamur Aspergillus Perusak Gaplek Oramahi, H. A.; Sumardiyono, Christanti; Pusposendjojo, Nursamsi; Haryadi, Haryadi
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 15, No 1 (2010): February 2010
Publisher : Universitas Atma Jaya Yogyakarta

Dried cassava is a suitable host for the growth and development of the genus Aspergillus. Fungi reported to grow on dried cassava were A. clavatus, A. flavus, A. foetidus, A. fumigatus, A. niger, A. ochraceus, A. oryzae, A. tamarii, A. zonatus, dan Aspergillus sp. From those species, A. flavus is the most important species because of its toxigenic characteristic on agricultural product. This study was conducted to study dominant species of Aspergillus causing the most severe deterioration on stored dried cassava and causing the highest deterioration in dried cassava. The isolates were then cultured for determination of dominant species. The in vitro experiment was to obtain the species of Aspergillus that is resulted in highest change of dried cassava spoilage. Based on relative index frequency (Rif) and presence index of the fungus (Pif), A. flavus was the most dominant species and causing the highest deterioration on the dried cassava.

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Brigitta Laksmi Paramita

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Editorial Address
Fakultas Teknobiologi, Universitas Atma Jaya Yogyakarta, Jalan Babarsari No. 44, Sleman, Yogyakarta 55281, Indonesia

Location
Kota yogyakarta,

Daerah istimewa yogyakarta

INDONESIA

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Home Page

OAI Link

Editorial Team

Contact

Reviewer

Google Scholar

Contact Name Brigitta Laksmi Paramita

Contact Email brigitta.laksmi@uajy.ac.id

Phone +6282329549978

Journal Mail Official journal.biota@gmail.com

Editorial Address Fakultas Teknobiologi, Universitas Atma Jaya Yogyakarta, Jalan Babarsari No. 44, Sleman, Yogyakarta 55281, Indonesia

Location Kota yogyakarta, Daerah istimewa yogyakarta INDONESIA

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Contact Name
Brigitta Laksmi Paramita

Contact Email
brigitta.laksmi@uajy.ac.id

Phone
+6282329549978

Journal Mail Official
journal.biota@gmail.com

Editorial Address
Fakultas Teknobiologi, Universitas Atma Jaya Yogyakarta, Jalan Babarsari No. 44, Sleman, Yogyakarta 55281, Indonesia

Location
Kota yogyakarta,

Daerah istimewa yogyakarta

INDONESIA