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Brigitta Laksmi Paramita
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Fakultas Teknobiologi, Universitas Atma Jaya Yogyakarta, Jalan Babarsari No. 44, Sleman, Yogyakarta 55281, Indonesia
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Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati
Published by Universitas Atma Jaya Yogyakarta
ISSN : 25273221     EISSN : 2527323X     DOI : doi.org/10.24002/biota
Core Subject : Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology
Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati merupakan jurnal ilmiah yang memuat hasil-hasil penelitian, kajian-kajian pustaka dan berita-berita terbaru tentang ilmu dan teknologi kehayatian (biologi, bioteknologi dan bidang ilmu yang terkait). Biota terbit pertama kali bulan Juli 1995 dengan ISSN 0853-8670. Biota terbit tiga nomor dalam satu tahun (Februari, Juni, dan Oktober).
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Pertanian dan Biologi (Lain-Lain)
Articles 1,193 Documents
 91   92   93   94   95 
Peningkatan Produksi Asam Glutamat Corynebacterium glutamicum dengan Penambahan Penisilin pada Fase Logaritmik Exsyupransia Mursyanti; Sri Lestari
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 10, No 2 (2005): June 2005
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v10i2.2843

Abstract

One way to increase production and excretion of glutamic acid was to increase cell's permeability. Penicillin has a potency to change the cell permeability by inhibitng cell wall synthesis. However, penicillin treatment was effective only for actively dividing cells. Therefore, such a research was done to study on the time of penicillin treatment to the medium, so that it can be found optimal cell biomass to produce maximum glutamic acid. The cell utilized in the research was Corynebacterium glutamicum IFO 12168 that was in batch cultured. Concentration of penicillin added was 5 unit/ml and treated at incubation periods 12, 14, 16, 18, and 20 hours, respectively, after inoculation. The steps of the research were as follows purification test, growth pattern, and glutamic acid production. Parameters measured at the end of the fermentation were cell biomass, reduced sugar concentration, medium’s pH, and glutamic acid concentration. Data was analysed utilizing Anova and the significant difference between treatments were tested using Duncan’s Multiple Range Test (DMRT). The growh pattern shown that logarithmic phase was reached at 2 to 22 hrs of incubation periods, therefore the treatment of penicillin was given at 12, 14, 16, 18, and 20 hrs of incubation periods. Cell biomass produced was corelate with the concentration of reduced sugar in the medium. Measured pH of the medium at the end of the fermentation was on the pH range for the growth of C. glutanicum. The research concluded that Penicillin treatment was able to increase significantly the glutamic acid production compatred to control treatment. Time accuracy of penicillin treatment to produce maximum glutamic acid (154319,60 µg/ml) was on 18 hrs of incubation period.
Aktivitas Antibakteri Ekstrak Metanol Buah Mbosi (Dysoxylum gaudichandianum (A.Juss) Miq.) dan Penapisan Senyawa Kimianya Praptiwi Praptiwi; Mindarti Harapini
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 10, No 2 (2005): June 2005
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v10i2.2844

Abstract

Mbosi (Dysoxylum gaudichandianum (A.Juss) Miq.) belongs to Meliaceae family, Its potential bioactivity had not been known although the other plants from the same family had been known as natural insectiside or medicinal plants. Phytochemical screening showed that mbosi fruit contained fat, fatty acid, sterol, triterpenoide, tannin, peroxide sugar, alkaloid, steroid glycoside and flavonoid. Identification of mbosi fruit chemical compounds by GC-MS showed that it contained 16 compounds. Compounds with the concentration more than 5 % were butyl cellosolve (11.1%), erythritol (8.48%), glycerol (10.32%), hexadecanioc acid palmitinic ester (16.2%), oleic acid (65.3%) and ethyl oleat (6.2%).  In-vitro antibacterial test on innoculated Mueller Hinton Agar to seven bacteria isolates (Salmonella typhimurium, S. typhii, Eschechiria coli ATCC 25923, Pseudomonas sp., Staphylococcus aureus ATCC 25922, S. epidermidis, and Bacilus subtilis)  showed that 20% methanol extract  inhibit only the growth of  Staphylococcus aureus.
Induksi Pembungaan Rambutan dengan Aplikasi Paklobutrazol Theresia Prawitasari; Dorly Dorly; Sri Wahyuni
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 10, No 2 (2005): June 2005
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v10i2.2845

Abstract

Rambutan fruit have a great potential to be developed as an exported commodity. The constraint is the existence of biannual bearing characteristic of the plant, which is an alternation of fruit yield between on year and off year. This condition resulted in a problem in product availability continuity. The aim of this research was to induce rambutan plant flowering in the off year with the application of paclobutrazol. There were three doses of paclobutrazol treatment including 0 g/tree, 1.5 g/tree, and 3 g/tree. Bud dormancy as an implication of paclobutrazol application were broken by the application of KNO3 at 20 g/l concentration. The experiment was arranged in completely randomized factorial design 3X2 in triplicate. The result showed that paclobutrazol treatment was able to induce rambutan flowering in the off year. Paclobutrazol treatment significantly increased the number of generative buds. Based on the generative bud percentage, the higher doses of paclobutrazol application the stronger stimulus of flowering. Paclobutrazol treatment also reduced the inflorescences length and increased the chlorophyll content of the flowering support leaves. It seems that application at dose 3 g/tree caused bud dormancy but dose 1.5 g/tree did not. Bud dormancy as a result of paclobutrazol application could be broken by the KNO3 application. The optimum dose to induced rambutan flowering was 1.5 g/tree because this dose seems did not caused bud dormancy and gave a higher number of generative buds compared to 3 g/tree dose.
Peluang Penggunaan Spermatozoa Epididimis Yang Dikoleksi Setelah Kematian Sebagai Sumber Sel Gamet pada Anjing I Ketut Puja; IGN Trilaksana; Rudy Lontoh
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 10, No 2 (2005): June 2005
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v10i2.2846

Abstract

The purpose of this study was to determine the effect of postmortem time on percentage of life epididymal sperm from postmortem canine caudae epididymides. A total of 9 dog were used and divided into three group. T0 was control group, T1, 3 hours postmortem and T2, 6 hours postmortem. This way, samples were obtained at different times postmortem. Sperm were extracted from the caudae epididymis by means of cuts. The result showed that the percentage of life sperm were 67,16 ± 5.67(T0), 46.33± 5.60 (T1) and 24.00 ± 4.35 respectively. We could appreciate that percentage of life was affected by postmortem time. There was significant decrease life sperm recovered from epididymides postmortem (P<0.01). In conclusion, epididymal sperm from dog undergo decrease of percentage of life, but it could stay acceptable within many hours postmortem. We intepreted these data to indicate that it may still be possible to obtain viable spermatozoa many hours later.
Kajian Awal Pemanenan Siput Laut (Gastropoda) di Pantai Krakal, Yogyakarta III: Aktivitas Wisatawan Santi Ari Andi; Felicia Zahida; B. Boy Sidharta
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 10, No 2 (2005): June 2005
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v10i2.2847

Abstract

This study wanted to elucidate the role of tourists activities to the beach such as Krakal Beach, Yogyakarta. The method used in this research was direct interview to the visitors using accidental sampling with the help of prepared questionnaire. This study showed that 86% visitors were in their school age. Person in this age like such a challenge like what they met during beach visit. Those make the visitation frequency quite high by repeating their visit (53,5%). Shells and shells handicrafts has been proven to be the reason why visitors come back to visit the beach (79%). Apparently, visitors agree that the snails’ forms and colors are attactive (70,9%), beautiful and unique (75,6%), but only 14% said that it shouldn’t be disturb by human activities. Their attractiveness to the snails did not make them to collect intentionally, enough for them to see and let them go (66,2%). They did not agree that visitors collected them (51,2%), but they agree harvesters make a souvenirs and get an income from this activity (67,4%). In short, they were much environment friendly (57%) rather than economic friendly (43%).  
Pemanfaatan Oleoresin Jahe (Zingiber officinale) untuk Mengatasi Kelainan Antioksidan Intrasel Superoxide Dismutase (SOD) Hati Tikus Di Bawah Kondisi Stres Tutik Wresdiyati; Made Astawan; I Ketut Mudite Adnyane; Renny Candra Prasetyawati
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 10, No 2 (2005): June 2005
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v10i2.2848

Abstract

This study was conducted to evaluate the activity of ginger oleoresin (Zingiber officinale) on the intracelluler antioxidant-superoxide dismutase (SOD) in the liver of rats under stress condition. A total of twuenty five Wistar rats were used for this study. They were divided into five groups ; (1)K (control), (2) S (stress), (3)O + S (oleoresin followed by stress), (4) S + O (stress followed by oleoresin and (5) O + S +O (oleoresin followed by stress then oleoresin). The dose of oleoresin is 60mg/Kg/BW/day for seven days. Stress condition was done by five days fasting and smimming for five minutes/day, while drinking water was provided ad libitum to all groups. The results showed that ginger oleoresin significantly decreased malonaldehyde (MDA) and elevated SOD activity. The immunohistochemical evaluation also showed that ginger oleoresin increased the content of copper,zinc-superoxide dismutase (Cu,Zn-SOD) in the liver of rats under stress condition. These effects were showed in the tissues of rats treated by ginger oleoresin before or after stress or combination of both.
Studi Komparasi Keanekaragaman Benthos Di Waduk Sempor, Waduk Kedungombo dan Waduk Gajahmungkur, Jawa Tengah (Short Communication) Wibowo Nugroho Jati
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 10, No 2 (2005): June 2005
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v10i2.2849

Abstract

Selama ini pengelolaan sumberdaya alam hanya didasarkan pada pemenuhan aspek ekonomi serta hubungan yang bersifat fisik (physical interrelationship). Hal ini dapat diperhatikan adanya kecenderungan pengelolaan sumberdaya alam sebatas program konservasi lingkungan fisik seperti hutan, tanah, air dan bukan pengelolaan sumberdaya alam yang bertujuan untuk keberlanjutan sumberdaya alam (natural resources sustainability). Dengan demikian maka dapat dipastikan lambat laun atau cepat akan terjadi degradasi atau penurunan kualitas lingkungan yang pada akhirnya dapat menimbulkan bencana ekologis (Hadisusanto et al., 2003).
Dengan Rumput Laut, Membangun Negara (Kajian Buku) Boy Rahardjo Sidharta
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 10, No 2 (2005): June 2005
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v10i2.2850

Abstract

Rumput laut (seaweed) adalah biota laut yang mungkin paling dikenal manusia sejak lampau (setelah ikan, tentunya). Manfaat rumput laut bagi manusia juga sangat bervariasi, mulai dari sumber makanan hingga obat-obatan. Di dunia moderen sekarang ini, rumput laut juga semakin dikenal berkat hasil-hasil penelitian ilmiah yang dilakukan manusia terhadap biota ini.
Variasi Morfologi dan Jenis Inang dari Cyclodontostomum purvisi (Adam, 1933) (Nematoda: Strongyloidea) di Indonesia Endang Purwaningsih
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 8, No 3 (2003): October 2003
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v8i3.2851

Abstract

Cyclodontostomum purvisi  (Adam, 1933) is a nematode infects small mammals belongs to family Muridae  (Rattus spp and Maxomys spp)  in  Kalimantan, Sulawesi and Java. There are some morphological variation on those nematode such as the number of corona radiata elements and head orientation (the angle between cephalic collar and sagital body axis). The angle is stronger in the specimens from Kalimantan and decreasing in the specimens in Sulawesi and Java. R. exulans  from Kalimantan and R. tanezumi from Sulawesi are the new host record for  C. purvisi in Indonesia.
Bioaugmentasi Benzena Tanah Tercemar Hidrokarbon yang Dibiodegradasi secara in vitro dengan Menggunakan Bacillus Sp. Strain U41 dan U44 Agus Irianto; Oedjiono Oedjiono; Agus Riyanto; M. Syamsul Komar
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 8, No 3 (2003): October 2003
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v8i3.2852

Abstract

Soil pollution by substances such as benzene can cause serious problems such as aquifer contamination and reduction of the biodiversity of organisms. A number of microorganisms are capable to degrade such substances naturally. However, introduction of any other microorganisms and or nutrient (bioaugmentation) are necessary in order to improve the biodegradation rate. This study examined the effect of introducing promising local strains of Bacillus namely U41 and U44, and urea addition at concentration 0.25% w/v. The parameter measured was benzene, pH, microbial number, and CO2. The best result was revealed from bioaugmentation of mixture of U41 and U 44. However, that result was not significantly difference with the use of single either strain U41 or U44, respectively.

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