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Cropsaver : Journal of Plant Protection

cropsaver
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Published by Universitas Padjadjaran

ISSN : - EISSN : 26215756 DOI : -

Core Subject : Science,

Biochemistry, Genetics & Molecular Biology

The objective of the CROPSAVER Journal is to present articles containing interesting and strategic issues. This journal discusses the discussion of articles with discussion rooms in the fields of plant pests and diseases, technology for pest control, taxonomy, insect physiology, biological insects, toxicology of pesticides, settlement pests that help research, research and research.

Arjuna Subject : -

Articles 107 Documents

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Effectiveness of Storage Time Formulation of Bacillus Thuringiensis Against Aedes aegypti Larvae (Linnaeus, 1757) Melanie Melanie; Mia Miranti Rustama; Inriyani Sintia Sihotang; Hikmat Kasmara
CROPSAVER Vol 1, No 1 (2018)
Publisher : Departemen Hama dan Penyakit Tumbuhan Fakultas Pertanian Universitas Padjadjaran

Aedes aegypti is known as the main vector of dengue fever in Indonesia which causes a high number of deaths and become an outbreak every year. This disease is generally controlled by breaking the life cycle of mosquitoes with restricted breeds and using synthetic insecticides. So far biological agents from natural enemies and natural pathogens have been developed to anticipate the negative effects of synthetic insecticides. Bacillus thuringiensis is well known as entomopathogenic bacteria that can control various insect pests of cultivated plants and vector insects, such as Ae.aegypti. B. thuringiensis can form endospores that produced toxin to Ae.aegypti, while the advatages are safe against non-target organisms such as natural predators and parasites, safe for mammals and humans and do not pollute the environment. Effective duration of B. thuringiensis in infecting Ae. aegypti is an important thing to be studied, moreover it is important to know the effectiveness of the expired formulation on the market and the residual effect. This ensures that the use of B. thuringiensis remains safe and does not create new problems such as Ae. aegypti resistance. The experimental research was used bioassay method, toxicity test was performed B. thuringiensis expired and non expire as comparison at concentration 0 ppm (control), 0,3 ppm; 0.1 ppm; 0.07 ppm; 0.05 ppm; 0.03 ppm; 0.01 ppm. The parameters observed were mortality for 48 hours of toxicity test that results were indicated by LC 50, LC90 and recidual values. The results obtained were LC50 and LC formulations B.thuringiensis expired 0.305 ppm and 1.513 ppm much higher than the non expire LC50 and LC formulations of 0.0363 ppm and 0.8971 ppm. The recidual effect of expired B. thuringiensis on larvae Ae. Aegypti was only effective until day 12, whereas B. thuringiensis that non expire formulation was effectively until day 14.Keywords : Ae. aegypti, B. thuringiensis, mortality, recidual values, expired formulation

Effect of Temperature, Storage Time, The Residual Test of Neem Oil Formulation (Azadirachta indica A. JUSS) and Bitung Formulation (Baringtonia asiatica) to Its Toxicity Against Large Cabbage Heart Caterpillar (Crocidolomia pavonana F) Neneng Sri Widayani; Afifah Nashirotul Haq; Lindung Tri Puspasari; Yusup Hidayat; Danar Dono
CROPSAVER Vol 1, No 1 (2018)
Publisher : Departemen Hama dan Penyakit Tumbuhan Fakultas Pertanian Universitas Padjadjaran

Effectiveness of pesticide affected by many factors such as physical environment and duration of storage. This study aims to determine the effect of temperature and storage time of formulation of neem 50 EC and residual effect of formulation of neem 50 EC and formulation of bitung 30 SC against C. pavonana larvae. The formulations tested on the experiment of effect storage time and temperature test using the newly prepared, neem formulation has been stored for 19 months at room temperature 24.6-31.7oC, and stored in low temperature (4oC) as well as controls. The toxicity of the neem oil formulation was tested at concentrations of 1%, 2%, and 3%. The experiment of residual effect was performed by spraying of the formulation on broccoli leaf, then the leaf samples were taken on days 0,1,2,3,5,7,9,11, and 13 days after application of insecticide. The results of the research indicated that storage at low temperatures (temperature 4oC) for 19 months can maintain the toxicity of the neem formulation to C. pavonana larvae. while storage at room temperature for 19 months resulted in a decreased toxicity. The residual activity of the neem formulations at concentration of 0.8% and 2.4% were not caused mortality of C. pavonana larvae at the residual age of 13.34 days and 14.66 days. The activity of Bitung formulation at concentration of 1.1% and 2.8% were not caused mortality of C. pavonana larvae at the residual age of 11.33 days and 16.26 days.

Toxicity of Barringtonia asiatica Seed Extract Against Chilo sachariphagus Rohendi Rohendi; Danar Dono; Andang Purnama
CROPSAVER Vol 2, No 1 (2019)
Publisher : Departemen Hama dan Penyakit Tumbuhan Fakultas Pertanian Universitas Padjadjaran

Barringtonia asiatica is one of the plants that the seeds extracts has insecticidal properties. However, the bioactivity of this seed extract has not been tested against Chilo sacchariphagus larvae which is the main pests in sugar cane. The treatment was carried out at several levels of concentration of methanolic seed extract of B. asiatica. The 15 cm of fresh sugarcane shoot dipping into the extract solution in some second. After the solution of extract on the sugarcane shoot dried, the first instar of larvae infested to the sugarcane shoot and then put into 20 cm test tube with 3 cm of diameter. The duration of feeding on treated sugarcane shoot was two days and on the following day the larvae were fed on sugar cane shoots until the survived larvae reached the 5th instar. The results of the experiment indicated that B. asiatica seed extract was toxic to C. sacchariphagus with an LC50 value of 0.398% at 16 days after treatment. Besides toxic, B. asiatica seed extract also has atifeedant activity, and inhibits the growth of immature of C. sacchariphagus in all tested concentrations.Keyword: Toxicity, antifeedant, lethal concentration, Barringtonia asiatica, Chilo sachariphagus

Susceptibility of Brown Planthopper (Nilaparvata lugens Stal.) from Cipunagara and Banyuwangi Population to Neem Oil Formulation Neneng Sri Widayani; Danar Dono; Yusup Hidayat
CROPSAVER Vol 1, No 2 (2018)
Publisher : Departemen Hama dan Penyakit Tumbuhan Fakultas Pertanian Universitas Padjadjaran

Brown Planthopper is one of the pests that cause problems in rice production. This study was conducted to know the tendency of using a kind of insecticide and to examined the susceptibility of brown planthopper from field population (Cipunagara, West Java) compared to standard population (Banyuwangi, East Java) to formulation insecticide of neem oil 50 EC. Observations wereconducted on Cipunagara farmers to find out the kind of insecticide used and the number of insecticide applications in one season. the susceptibility test to neem oil 50 EC formulation was conducted by root dipping method. Brown planthopper was feed on treated plant for two days and then the next day the Brown Planthopper was fed on rice plant without treatment until the eighth day. Test insects used are brown planthopper instar 4th. Observations of insect mortality test for ten days with an interval of every 24-hour observation. Susceptibility values for neem oil insecticide formulations were determined based on the resistance ratio (RR) by comparing the LC50 value of the field population and standard population. Result of the observations showed that the farmers in the Padamulya village, Cipunagara district generally using synthetic insecticides to control of this pest. The active ingredient of most mentioned was imidacloprid with a lot of different applications. The result of examination of insecticide formulation of neem oil 50 EC using the root dipping method showed that a slow death trend and the highest mortality at eight days after application. The LC50 value of neem oil 50 EC insecticide against brown planthopper in the field population (Cipunagara) was 0.36% and RR 0.98. The result indicated that brown planthopper origin from Cipunagara was still susceptible to neem oil 50 EC insecticide formulation.Keyword: Neem oil formulation, Susceptilbility, Resistance ratio, Brown Planthopper

The Potential and Application Frequency of Yeast from Tomato and Cogon Grass Leaves to Suppress Powdery Mildew Disease in Tomato Noor Istifadah; Nurul Ihsani; Sri Hartati
CROPSAVER Vol 1, No 2 (2018)
Publisher : Departemen Hama dan Penyakit Tumbuhan Fakultas Pertanian Universitas Padjadjaran

Powdery mildew (Oidium sp.) is important disease in the greenhouse tomato production. One of environmentally-friendly control method of the disease is biological control. Yeast from phyloplane of weeds such as cogon grass are potential as biocontrol agents. This study evaluated the potential of yeast isolated from the leaves of cogon grass and wild tomato to suppress powdery mildew of tomato. The samples were obtained from several areas in Sumedang and Bandung, West Java. The yeast isolates were selected on their abilities in controlling powdery mildew in tomato seedlings. The selected isolates were used for further experiment examining the effects of frequency of yeast application on the disease control. This experiment was arranged in Randomized Complete Block Design with 12 treatments and three replications. The treatments were three yeast isolates, applied once before pathogen inoculation, applied regularly every week or two weeks, the check and fungicide treatment. The results showed that among 13 yeast isolates obtained there were three isolates that suppressedthe development of powdery mildew disease by 61.0%- 68.6%. In further experiment, relatively highest disease reduction (58,6%) was showed in the application of yeast isolate from wild tomato leaves (TB1 isolate) every week.Keywords : biological control, phyloplane, Oidium sp., wild tomato

Laboratory Evaluation of Neem formulation bioactivity against Crocidolomia pavonana F. larvae Raden Arif Malik Ramadhan; Neneng Sri Widayani; Lindung Tri Puspasari; Yusup Hidayat; Danar Dono
CROPSAVER Vol 1, No 1 (2018)
Publisher : Departemen Hama dan Penyakit Tumbuhan Fakultas Pertanian Universitas Padjadjaran

This study aims to evaluate the bioactivity of the formulation of Neem 50 EC against Crocidolomia pavonana larvae in the laboratory. The study using six treatments and three replications. The treatment consisted of the formulation of neem 50 EC at concentrations of 0.3%, 0.5%, 0.8%, 1.3%, 2.3%, and control. The treatment was done by leaf feeding method and tested on C. pavonana instar II. The results of the research showed that the formulation of neem 50 EC at concentration of 2.3% caused mortality of C. pavonana larvae with mortality rate of 95% and has LC50 value aqual to 0.83%. Neem formulation can inhibit the development of C. pavonana larvae from instar I to instar IV, decrease food consumption, and decrease the dry weight of C. pavonana larvae instar IV.Keywords: Azadirachta indica, Bioactivity, Crocidolomia pavonana, neem formulation

Effect of the Initial Temperature of Extraction of Neem Cake (Azadirachta indica A. JUSS) on its Toxicity on Crocidolomia pavonana (F.) Larvae Fhera Hardiani; Danar Dono; Ceppy Nasahi
CROPSAVER Vol 2, No 1 (2019)
Publisher : Departemen Hama dan Penyakit Tumbuhan Fakultas Pertanian Universitas Padjadjaran

Crocidolomia pavonana is an important pest that attacks the Brassicaceae. Neem cake is waste from neem seed pressing that can be used as a botanicals insecticide to control insect pests. The effect of the initial temperature of extraction can have an impact on the toxicity of the extract. The aim of this study was to determine the best initial temperature for the extraction of neem cake on mortality, larval development time, feed consumption, larval weight and survival of larvae into pupae and adult. The experimental method used in this research was Randomized Completely Design (RCD) with 4 treatments and 4 replication, i.e. control, extraction of neem cake at initial temperature of 30°C, initial temperature 50°C, and initial temperature 70°C which allows two concentrations of 1% and 3%. The results of the research with the best toxicity were shown in the initial extraction temperature of 50°C at concentrations of 1% and 3% with the mortality reaching 82.5% and 90%. The initial temperature extraction of 50°C also showed lengthened of the larval development time, decreased feeding activity, larval weight and survival of larvae to develop into pupae and imago.

Assessment of Sarocladium oryzae Growth Inhibition, the Causal Agent of Rice Sheath Rot Disease, Using Methanol Extract of Binahong Leaves Endah Yulia; Silviya Rahma Dhiya Syafira; Fitri Widiantini; Wawan Kurniawan
CROPSAVER Vol 2, No 1 (2019)
Publisher : Departemen Hama dan Penyakit Tumbuhan Fakultas Pertanian Universitas Padjadjaran

Sheath rot disease caused by Sarocladium oryzae is an important disease on rice. The pathogen that infects uppermost leaf sheath enclosing to the young panicles can cause yield losses up to 85%. Though the disease is commonly controlled using synthetic fungicides, the use of natural products that safer to the environment have been enormously explored. The research aimed to test growth inhibition of S. oryzae colony using methanol leaf extract of binahong (Anredera cordifolia). The research was carried out from June until December 2017 at the Laboratory of Biotechnology Plant Protection, Department of Plant Pest and Diseases, Faculty of Agriculture, Universitas Padjadjaran. The method used was Randomized Complete Design consisted of six treatments and four replications. The treatments were five concentrations of binahong leaf extract of 0.125%, 0.25%, 0.5%, 1%, 2% and one control treatment. The result showed that binahong leaf extract did not inhibit the growth of S. oryzae. The highest inhibition percentage of 4.55% at 0.25% concentration was considered to be a low inhibition category. Although the colony growth of S. oryzae was not significantly inhibited, the extract caused hyphal abnormalities. This actually shows the antifungal potency of the binahong leaf extract that needed further examination with more adequate methods.Keywords : Anredera cordifolia, botanical pesticide, rice sheath rot

Toxicity of Neem Cake Extract (Azadirachta indica) to Aphis glycine on Soybean Plant Ratmaneli Ratmaneli; Danar Dono; Vira Kusuma Dewi
CROPSAVER Vol 2, No 1 (2019)
Publisher : Departemen Hama dan Penyakit Tumbuhan Fakultas Pertanian Universitas Padjadjaran

Aphis glycine is one of the soybean pests that can develop rapidly and cause yield loss. Neem is a plant known as botanical incesticide. This study tested the toxicity of neem cake against A. glycine and its effect on increasing tannin content in the soybean plant. The effect of neem cake extract to the mortality of A. glycine was tested by spray method and root dipping method. The application was carried out on four-week-old soybean plants with 10 individual of adult A. glycine as test insects. Observations were carried out every day for 8 DAT (day after treatmen) by counting the population of A. glycine. The testing of tannin levels and azadirachtin content is done by applying neem cake extract using root dipping method. The treatment is divided into two groups, which are infested plants and non-invested plants by A. glycine. The leaf of soybean (fourth day after application) was taken as samples for tannin and azadirachtin analysis. The results showed that the extract of neem cake at the spray method caused the death of A. glycine up to 97% at a concentration 2.5%. The root dipping method could make mortality of nymphs (new born) 100% at concentrations of 0.1% and 0.125% at 8 DAT , but cannot make mortality to adult of A. glycine. The tannin content in soybean plants (with A. glycine and without A. glycine invested) was increased after 0.125% neem cake application. Azdirachtin uptake was not detected at concentrations of 0.125% and 0.250% at 5 DAT. Neem cake extract can suppress A. glycine population in soybean plants and increase of tannin content in plants.

Synergistic Activity of Mixtures of Piper aduncum Fruit Extract and Three Microorganism-Derived Insecticides against the Diamond Back Moth, Plutella xylostella Wanda Russianzi; Djoko Prijono
CROPSAVER Vol 2, No 1 (2019)
Publisher : Departemen Hama dan Penyakit Tumbuhan Fakultas Pertanian Universitas Padjadjaran

Plutella xylostella is an important pest of Brassicaceae vegetable crops. Frequent use of insecticides containing single active ingredients can cause resistance in P. xylostella. This work was done to evaluate the synergism between Piper aduncum fruit extract and three microorganism-derived insecticides, i.e. abamectin, chlorfenapyr, and spinetoram, against P. xylostella from Pacet District, Cianjur Regency. Results of leaf-residue feeding bioassays with 48-h feeding treatment showed that LC95 of abamectin, chlorfenapyr, and spinetoram at 96 h after treatment (HAT) was about 5.8, 7.0, and 1.9-fold higher than their respective field rates. Thus, based on leaf-feeding assays P. xylostella larvae from Pacet-Cianjur were not susceptible to abamectin and chlorfenapyr, but were still fairly susceptible to spinetoram. LC95 of P. aduncum extract at 96 HAT was 0.68% (w/v). Based on combination index at the LC95 level - 96 HAT, mixtures of P. aduncum extract with all three test insecticides were synergistic. Thus, P. aduncum extract is potential to be used as an alternative ingredient to increase the effectiveness of the three test insecticides against P. xylostella.Keywords: Botanical insecticides, cabbage pest, insecticide mixtures, microorganism-derived insecticides, synergism.

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