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Isna Rasdianah Aziz
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Department of Biology, Faculty of Science and Technology, Universitas Islam Negeri Alauddin Makassar. Jl. H.M. Yasin Limpo No. 36 Samata, Gowa, South Sulawesi, Indonesia. 92113
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Biogenesis: Jurnal Ilmiah Biologi
Published by Universitas Islam Negeri Alauddin Makassar
ISSN : 23021616     EISSN : 25802909     DOI : https://doi.org/10.24252/bio
Core Subject : Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology
Biogenesis: Jurnal Ilmiah Biologi is a peer-reviewed and open-access journal that publishes original scientific work with the advancement of tropical bioscience in Asia. The integration between Islam and tropical bioscience explicitly represents the Biogenesis: Jurnal Ilmiah Biologi as an academic publication for Islamic scientists, which could contribute to the advancement of tropical biology studies in the Islamic world. The journal invites research articles, short communication, critical and comprehensive scientific reviews concerning tropical biosciences in Asia, specifically in the following subjects: Islamic-integrated tropical bioscience Tropical biodiversity and conservation Biosystematics of tropical species Biochemistry and physiology of tropical species Ecological study and behavior of tropical species Tropical genetics, biotechnology, and bioinformatics
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Pertanian dan Biologi (Lain-Lain)
Articles 332 Documents
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Molecular Characterization of Anguilla from Cibereum and Sapuregel Rivers Segara Anakan Watersheds Cilacap, Central Java Agus Nuryanto; Dian Baghawati; Kusbiyanto Kusbiyanto; Moh Husein Sastranegara; Farida Nur Rachmawati
Biogenesis: Jurnal Ilmiah Biologi Vol 8 No 2 (2020)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v8i2.15532

Abstract

The taxonomic status of Anguilla species' in river watershed that empties into Segara Anakan Cilacap is uncertain, thereby making it difficult for further studies to be carried out to determine its genetic in that area. Therefore, this study evaluates Anguilla's taxonomic status and population genetic in Cibeureum and Sapuregel River watersheds. Data were obtained from molecular characterization study using cytochrome c oxidase 1, with fourteen Anguilla specimens collected from two sequenced watersheds. Taxonomic status was determined based on homology and divergence values and monophyly of the samples to the reference species. Meanwhile, genetic divergences among samples to the reference species were calculated based on the Juke-Cantor substitution model in DnaSP6. A homology test was performed using a basic local alignment search tool, with monophyly inferred from the cladogram, which was developed using neighbor-joining and maximum likelihood algorithms in MEGAX with 1000 pseudoreplicates and out-group comparison. Furthermore, population genetic was analyzed through polymorphism, haplotype, nucleotide diversity within the population, divergence, and genetic differences. All calculations conducted in Arlequin 3.5 had Anguilla samples comprising of high (99.23% to 99.84%) to low genetic divergences (0.224% to 1.127%). The result shows that cladogram with all Anguilla samples formed a monophyletic clade with A. bicolor, separated from their taxa. Furthermore, Anguilla samples from both watersheds have low genetic polymorphisms with medium to high haplotype and nucleotide diversity. The population's comparison proved that both populations have low genetic divergence, and no genetic difference based on variance analysis (p=0761). Therefore, Anguilla resources in river watersheds that empty into Segara Anakan are a single genetic conservation unit.
Ex-situ conservation of the native orchid Coelogyne rochussenii de Vriese from the Bukit Rimbang and Baling Wildlife Reserve Areas Pebra Heriansyah; Gusti Marlina
Biogenesis: Jurnal Ilmiah Biologi Vol 9 No 1 (2021)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v9i1.21274

Abstract

The native orchid Coelogyne rochussenii de Vriese is critically endangered, hence, conservative measures are needed to prevent extinction. Meanwhile, in-situ conservation is constrained by time, resources, and costs. Therefore, this study aims to identify the most effective method for ex-situ conservation, especially with basal medium and activated charcoal, to determine the fastest germination. The completely randomized factorial design of four different basal media treatments including Knudson C, Vacin and Went, Murashige and Skoog, and Hyponex + vitamin medium were used. These treatments were combined with the application of activated charcoal at four different levels, with concentrations of 0, 1, 2, and 3 g/L-1, meanwhile, the parameters observed include germination day and percentage, as well as contamination percentage. The results showed that the conservation of Native orchid C. rochussenii de Vriese using various growing media and activated charcoal had a significant effect on the growth of the embryo culture. Based on the results, the Hyponex + vitamin medium with 3 g/L-1 accelerated germination days to 29.33, increased germination percentage to 92.06%, and reduced contamination to 0%.
ISSR based analysis of genetic variability of plantlets culture of pineapple (Ananas comosus L.) from Sipahutar, North Sumatera, Indonesia Fauziyah Harahap; Annisa Afiva; Miftahul Jannah; Eko Prasetya
Biogenesis: Jurnal Ilmiah Biologi Vol 9 No 1 (2021)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v9i1.17068

Abstract

Sipahutar pineapple is very popular in North Sumatra, because of the distinctive sweet taste and normal water content. Furthermore, it is large in size and has a yellow fruit skin color with greenish tips. However, the problem with Sipahutar pineapple production is the limited amount of good quality seeds. The utilization of in vitro culture techniques on pineapples allows the emergence of somaclonal variations, especially in plantlets that have undergone the subculture stage. This somaclonal variation is one of the problems in commercial seedling production, therefore, it is necessary to conduct an initial examination using the inter simple sequence repeat (ISSR) molecular marker. This study aimed to determine the genetic and primary stability of ISSR, which can be used in pineapple plants native to Sipahutar. The methods used include sample preparation, DNA isolation, primer optimization, ISSR primer amplification by PCR method, and electrophoresis. Furthermore, a total of 15 samples were amplified with six ISSR primers, and the data were analyzed by cluster method using the NTSYS-PC software. The final result was visualized in a dendrogram and analysis of diversity was conducted using GenAlex. The results showed that the level of genetic variability of the Sipahutar pineapple, which has undergone in vitro culture using six ISSR molecular markers was 76-97%. Meanwhile, the genetic variability level of the native to Sipahutar pineapple can be influenced by the long culture period and the use of N6-benzyladenine. The primers can be used to observe the genetic variability, except for ISSR 25 with a PIC value of 0.000.
Molecular characters of melon (Cucumis melo L. ‘Kinaya’) using inter simple sequence repeat Budi Setiadi Daryono; Puti Hana Ramadhani; Esty Nidianti
Biogenesis: Jurnal Ilmiah Biologi Vol 9 No 2 (2021)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v9i2.15997

Abstract

Cultivar ‘Kinaya’ is a melon produced from breeding between ‘Sonya’ and ‘Kinanti’. This study on phenotypic and molecular characters is carried out to support ‘Kinaya’ as a superior cultivar. Our study aimed to analyze the genetic variation of the melon ‘Kinaya Bulat Kuning’ and ‘Kinaya Kuning Lonjong’ and its parental ‘Sonya’ and ‘Kinanti’. The research was conducted in the Greenhouse of Mutihan, D.I.Yogyakarta, and the Laboratory of Genetics and Breeding, Faculty of Biology, Universitas Gadjah Mada. Quantitative characters were determined by morphometric comparisons, fruit weights, and the number of seeds. Qualitative characters included fruit color, fruit skin color, aroma, texture, and taste. The molecular characterization method was inter simple sequence repeat (ISSR) and included DNA isolation, spectrophotometry, amplification of DNA target using PCR, and visualization of DNA target. Molecular characters were analyzed using spectrophotometry and visualization of DNA bands by electrophoresis using the MVSP 3.1 program. PCR used four random primers such as UBC 807, 809, 810, and 812, which obtained 11 polymorphic and 12 monomorphic DNA bands with a polymorphism rate of 47.8%. It is known that the cultivar ‘Kinaya’ has a similarity of 72% with its inductees ‘Sonya’ and ‘Kinanti’.
Growth and physiological response of rice ‘Inpari 35’ under salinity stress and application of silicate fertilizer Aulia Noor Ramadhani; Diah Rachmawati
Biogenesis: Jurnal Ilmiah Biologi Vol 9 No 2 (2021)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v9i2.20754

Abstract

Rice is an important staple food in Indonesia. Crop areas can be expanded to boost productivity by utilizing marginal lands, which are saline. This study aims to study the growth and physiological response of rice ‘Inpari 35’ to the application of silicate fertilizer under salinity stress conditions. This study used a completely randomized design (CRD) with two factors. The first factor is the difference in NaCl salt concentration consisting of N0: 0 mM; N1: 37.5 mM; N2: 50 mM, while the second factor is the difference in the concentration of silicate fertilizer (CaSiO3) consisting of S0: 0 mM; S1: 1 mM and S2: 2 mM. Each treatment combination was repeated three times. Observed data were analyzed by analysis of variance (ANOVA). A significant difference between treatments is continued with Duncan's multiple distance test at a 95% confidence level. The results showed that NaCl treatment significantly (p<0.05) inhibited the growth of rice ‘Inpari 35’, which was indicated by a decrease in the plant height and number of leaves. The NaCl treatment caused a reduction in the levels of chlorophyll, carotenoids, proline, membrane stability index (MSI), and relative water content (RWC). The interaction between NaCl treatment and CaSiO3 showed significant differences in physiological parameters by increasing the levels of chlorophyll, carotenoid, proline, membrane stability index, and relative water content.
Effect of biofertilizer and salinity stress on productivity and vitamin C levels of Amaranthus tricolor L. Nadhifa Athaya Khairunnisa; Dwi Umi Siswanti
Biogenesis: Jurnal Ilmiah Biologi Vol 9 No 2 (2021)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v9i2.21629

Abstract

Abiotic stress is one of the elements that affect plant crop output due to its productivity and environmental factors. Salinity as abiotic stressors can impair plant growth, becoming a concern in the agriculture field in recent years. Biofertilizers are reported to be capable of overcoming salinity stress. Hence, it contains microbial elements in it, play a role for the nitrogen cycle in soil, which can result in increased plant growth. Therefore, this study aimed to analyze the effect of biofertilizer and NaCl as a salinity stress factor on the growth of Amaranthus tricolor L. The biofertilizer doses utilized in this study were 10 L/ha, 20 L/ha and 30 L /ha, each in combination with basic manure fertilizer. For the salinity factor, NaCl concentrations of 2500 ppm, 5000 ppm, 7500 ppm, and 10.000 ppm were employed. Environmental characteristics, plant height, number of leaves, root length, plant dry mass, chlorophyll and carotenoid content, as well as vitamin C, were all measured. The parameters were determined quantitatively. The chlorophyll and carotenoid contents were determined using a UV-vis spectrophotometer, while vitamin C levels were determined using iodometric titration. At a 95% level of confidence, the results were examined using the one-way ANOVA approach. The results indicate that a 30 L/ha dose of biofertilizer has an effect on the chlorophyll content and root length of plants, whereas a 10 L/ha dose has an effect on the carotenoid content. The highest amaranth growth was observed when 0 L/ha biofertilizer was combined with a 7500 ppm NaCl treatment, whereas the largest number of leaves was shown when 10 L/ha biofertilizer was combined with a 2500 ppm NaCl treatment. It could be concluded that while biofertilizer has no effect on plant growth parameters, it does increase plant productivity by raising chlorophyll and carotenoid levels.
Effect of biofertilizer on growth and metaxylem diameter of Amaranthus tricolor L. in salinity stress condition Okky Shavira Riesty; Dwi Umi Siswanti
Biogenesis: Jurnal Ilmiah Biologi Vol 9 No 2 (2021)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v9i2.22232

Abstract

Throughout history, agricultural sector in Indonesia has faced a shortage of land. As a result, we must take use of land that is still available, even if the conditions are unsuitable for plant growth, one of which is salinity-stressed land. Amaranth is a frequently cultivated plant in Indonesia (Amaranthus tricolor L.). This vegetable plant is commonly consumed as food due to its nutritional content and numerous health benefits. To cultivate amaranth on salinity-stressed land, additional nutrients are required to ensure that the plants continue to thrive. One of them is the provision of biofertilizers, a type of organic fertilizer that contains beneficial bacteria for plant growth. This study aimed to determinate the effect of biofertilizer application on the growth and diameter of the stem metaxylem of amaranth plants growing in a salinity-stressed environment. As a salinity stress treatment, NaCl was applied at doses of 0, 2500, 5000, 7500, and 10000 ppm. The diameter of the metaxylem was determined by making fresh preparations across amaranth stems. The biofertilizer application does not affect the height and number of leaves of the plant. However, as the dose of biofertilizer was increased, the value of the metaxylem diameter of the stem increased.
Stem volume equation for constructing local volume table of Pinus merkusii Jungh et de Vriese in Tana Toraja community forest Melewanto Patabang; Hardjanto Hardjanto
Biogenesis: Jurnal Ilmiah Biologi Vol 9 No 2 (2021)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v9i2.22281

Abstract

Pinus merkusii Jungh et de Vriese is a plant with high market and economic values due to providing a variety of products, including wood. Good management is needed to preserve pine forest products. The first step toward effective forest management is developing a management plan based on estimated stock data. The volume table used has a significant impact on the accuracy of the stock potential estimate. This study aims to determine the best equation model for compiling a local volume table for P. merkusii Jungh et de Vriese in Tana Toraja Regency. The research was conducted at the community pine forest in Gandang Batu Sillanan and Mengkendek districts, Toraja Regency. The number of sample measured was 100 trees. An analytical method was used to calculate the amount of tree volume on allometric equations. The equation for the stem volume was made using the allometric equation. The research results showed that diameters' mean, standard deviation, and sampling error values were 28.34 cm, 7.39 cm, and 1.45, respectively, heights were 23.77 m, 7.37 m, and 1.44; volume was 1.44 m3, 0.96 m3, and 0.19. The regression equation modelling showed that the best equation model for estimating volume P. merkusii Jungh et de Vriese based on height and diameter was ????=????????2????.
Monitoring of insect pollinators of mango (Mangifera indica L.) inflorescence based on citizen science Ipin Aripin; Topik Hidayat; Nuryani Y Rustaman; Riandi Riandi
Biogenesis: Jurnal Ilmiah Biologi Vol 9 No 2 (2021)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v9i2.23509

Abstract

Mango cross-pollination can be encouraged through the presence of pollinating insects, which can be investigated and observed through citizen science activities. This study aims to monitor the presence of insect pollinators of mango (Mangifera indica L.) inflorescence through citizen science activities. The data generated in the study can be used as a reference to determine population trends and the biodiversity of mango insect pollinators. A citizen science approach in participatory research was used to collect and identify the data. A total of 68 volunteer participants from two universities in west Java were involved in this study. The participants had to meet the requirements to have contracted ecology courses. Smartphones and insect identification guidelines and databases at https://www.discoverlife.org/ and https://www.inaturalist.org/ were used as a tool in this research. The identified data were submitted via google form (www.bit.ly/csmangga) and the Inaturalist application for publication. It was discovered that mango inflorescence insect pollinators comprised five orders, 26 families, and 39 species. Diptera and Hymenoptera orders are insects that have the biggest role in mango pollination, and Chrysomya sp. is an insect species found in almost all mango cultivars.
Nano-chitosan coating on maintaining the quality of postharvest chili pepper (Capsicum frutescens L.) Noor Laila Safitri; Erma Prihastanti; Sri Widodo Agung Suedy; Agus Subagio
Biogenesis: Jurnal Ilmiah Biologi Vol 9 No 2 (2021)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v9i2.23524

Abstract

Chili pepper (Capsicum frutescens L.) is a horticulture product with a limited shelf life due to quality degradation following harvest. One method of preserving the quality of chili peppers can be evaluated by using nano-chitosan, which combine chitosan and sodium tripolyphosphate (STPP) in certain ratio. The purpose of this study was to determine the impact of nano-chitosan on maintaining the quality of C. frutescens L., the optimal ratio of chitosan to STPP for preserving the quality of C. frutescens L. after harvest, and the shelf life of C. frutescens L. treated with nano-chitosan after harvest. This study employed a completely randomized design (CRD) and included four treatments: P0 (control), P1 (0.2% nano-chitosan, 1:3 ratio), P2 (0.2% nano-chitosan, 1:4 ratio), and P3 (0.2% nano-chitosan, 1:5 ratio), which conducted for 16 days. Weight loss, water content, texture, color, and percentage of damage are the research variables in this study. The ANOVA test was used to examine the data, followed by the DMRT test. The results indicated that nano-chitosan could maintain the weight, water content, texture, and color of chili peppers. The optimal ratio of chitosan to STPP to retain the quality of postharvest C. Frutescens L. is 0.2% nano-chitosan (chitosan: STPP= 1:5), and nano-chitosan can maintain the quality of C. Frutescens L. for up to 16 days.

Page 24 of 34 | Total Record : 332

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