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Isna Rasdianah Aziz
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Department of Biology, Faculty of Science and Technology, Universitas Islam Negeri Alauddin Makassar. Jl. H.M. Yasin Limpo No. 36 Samata, Gowa, South Sulawesi, Indonesia. 92113
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INDONESIA
Biogenesis: Jurnal Ilmiah Biologi
Published by Universitas Islam Negeri Alauddin Makassar
ISSN : 23021616     EISSN : 25802909     DOI : https://doi.org/10.24252/bio
Core Subject : Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology
Biogenesis: Jurnal Ilmiah Biologi is a peer-reviewed and open-access journal that publishes original scientific work with the advancement of tropical bioscience in Asia. The integration between Islam and tropical bioscience explicitly represents the Biogenesis: Jurnal Ilmiah Biologi as an academic publication for Islamic scientists, which could contribute to the advancement of tropical biology studies in the Islamic world. The journal invites research articles, short communication, critical and comprehensive scientific reviews concerning tropical biosciences in Asia, specifically in the following subjects: Islamic-integrated tropical bioscience Tropical biodiversity and conservation Biosystematics of tropical species Biochemistry and physiology of tropical species Ecological study and behavior of tropical species Tropical genetics, biotechnology, and bioinformatics
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Pertanian dan Biologi (Lain-Lain)
Articles 332 Documents
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Analysis of Amino Acid Sequence of SARS-CoV, SARS-CoV-2, and MERS-CoV Spike Glycoproteins: Preliminary Study for Obtaining Universal Peptide Vaccine Candidates Yani Suryani; Opik Taupiqurrohman
Biogenesis: Jurnal Ilmiah Biologi Vol 8 No 2 (2020)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v8i2.15696

Abstract

In the manufacture of universal peptide vaccines, it is necessary to analyze the amino acids of the various candidates. Therefore, this study aims to examine the amino acids of the spike glycoproteins of SARS-CoV, SARS-CoV-2, and MERS CoV. The method used is the alignment of the amino acid spike glycoprotein between SARS-CoV with SARS-CoV-2, MERS CoV with SARS-CoV-2, and SARS-CoV with MERS-CoV using web-based software water emboss. The analysis result showed that SARS and SARS CoV-2 were very similar with 87% similarity and 76.4% identity values. In contrast, SARS CoV-2 with MERS and SARS with MERS were very different, having similarity and identity values less than 70%. Therefore, it is reasonable to conclude that spike glycoprotein's peptide is only useful from attacks by the SARS-CoV and SARS-CoV-2 viruses.
Viability, productivity, and anatomical response of groundnuts (Arachis hypogaea L.) to biofertilizer-sludge biogas applications Nur Hidayah Pangestuti; Dwi Umi Siswanti
Biogenesis: Jurnal Ilmiah Biologi Vol 9 No 1 (2021)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v9i1.18037

Abstract

Groundnuts (Arachis hypogaea L.) is one of the essential food commodities in Indonesia. The use of biofertilizer has been applied to various types of crops. Meanwhile, the effect of using biofertilizer-sludge biogas on groundnuts is yet unknown. This study aims to analyze the seed viability and vigour, yield productivity, the anatomical response of groundnuts, and optimum concentration that could increase the values of each parameter. Treatments given include applying biofertilizer-sludge with 15 levels of treatment concentration compared to groundnuts without biofertilizer-sludge application as a control. The land was divided into 16 beds for each treatment consisting of control, biofertilizer from 10, 15, 30 L/ha, sludge from 12, 24, to 36 ml, and variations dosage of biofertilizer and sludge combined. The parameters observed for viability and vigour include the percentage of seed germination (GP), seed vigor index (SVI) for yield, the value of harvest index (HI), dry weight of the harvest, and root-shoot ratio (R/S). Anatomical responses were observed with stem diameter, stem’s metaxylem diameter, root diameter, root’s metaxylem diameter, and seed diameter. The biofertilizer-sludge results significantly affected HI, R/S values, stem diameter, root’s metaxylem diameter, and seed diameter. This research concluded that the application of biofertilizer-sludge did not significantly affect the seed viability and vigour and the dry weight of the harvest. The application of biofertilizer-sludge in various doses of concentration resulted in a decrease in the stem metaxylem diameter and root diameter compared to the control. A total of 10 L/ha biofertilizer + 24 ml sludge was an optimum concentration to increasing HI and R/S values. For the increasing stem, root metaxylem, and seed diameter, biofertilizer 30 L/ha + sludge 12 ml, sludge 24 ml, and biofertilizer 15 L/ha + sludge 12 ml were the optimum concentrations, respectively.
Strategy for Designing the Synthetic Gene Encoding Human papillomavirus Major Capsid L1 Protein for Heterologous Expression in Escherichia coli System Kartika Sari Dewi; Wien Kusharyoto
Biogenesis: Jurnal Ilmiah Biologi Vol 8 No 2 (2020)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v8i2.15805

Abstract

DNA is widely used to construct heterologously expressed genes. The adaptation of the codons to the host organism is necessary in order to ensure sufficient production of proteins. The GC content, codon identity and the mRNA from the translation site are also important in the design of the gene construct. This study performed a strategy for the design of synthetic gene encoding HPV52 L1 protein and several analyses at the genetic level to optimize its protein expression in the Escherichia coli BL21(DE3) host. The determination of the codon optimization was performed by collecting 75 HPV52 L1 protein sequences in the NCBI database. Furthermore, all the sequences were analyzed using multiple global alignments by Clustal Omega web server. Once the model was determined, codon optimization was performed using OPTIMIZER and the web server of the IDT codon optimization tool based on the E. coli B. The generated open reading frame (ORF) sequence was analyzed using Restriction mapper web server to choose the restriction site for facilitating the cloning stage, which is adjusted for pJExpress414 expression vector. To maximize the protein expression level, the mRNA secondary structure analysis around the ribosome binding site (rbs) was performed. A slight modification at the 5’-terminal end waa carried out in order to get more accessible rbs and increasing mRNA folding free energy. Finally, the construction of the synthetic gene was confirmed to ensure that no mutation occurs in the protein and to calculate its Codon Adaptation Index (CAI) and GC content. The above strategy, which leads to a good ORF sequence with the value of the free mRNA folding energy around rbs, is -5.5 kcal / mol, CAI = 0.787 and GC content 49.5%. This result is much better than its original gene. This result is much better compared to its native gene. Theoretically it is possible that this synthetic gene construct generates a high level protein expression in E. coli BL21 (DE3) under the regulation of the T7 promoter.
Assessment of the DNA Barcodes Characteristic of Phalaenopsis deliciosa based on matK, rbcL, and ITS Nada Nisrina Maulidya; Siti Rohimah; Zakiyah Ramadany; Tri Ratnasari; Mukhamad Su'udi
Biogenesis: Jurnal Ilmiah Biologi Vol 8 No 2 (2020)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v8i2.13278

Abstract

Indonesia has high biodiversity for plant species, including orchids with medicinal potential such as Phalaenopsis deliciosa. Generally, morphological characters, especially in flowers are used for orchids identification. However, when the plants are not in the flowering period, the identification becomes difficult. Therefore an alternative method, such as molecular identification (DNA barcoding) needs to be applied for the best solution. This research aims to identify and compare three markers (matK, rbcL, ITS) for their function as potential barcodes for Phalaenopsis deliciosa. This study was conducted by DNA amplification using three different markers set. The data were analyzed using Bioedit, BLAST, and ClustalX. The result found that the identity level of matK, rbcL, and ITS to other orchids species was 99-98%, 98%, and 94-96%, respectively. Furthermore, matK and ITS showed high specificity for Phalaenopsis deliciosa, and are therefore recommended as the best molecular identification marker of genus Phalaenopsis.
Identification of endophytic fungi in nutgrass (Cyperus rotundus L.) as solubilizing phosphate and indole-3-acetic acid producers Nur Kusmiyati; Septian Tri Wicaksono; Durrotul Maknuna
Biogenesis: Jurnal Ilmiah Biologi Vol 9 No 1 (2021)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v9i1.21216

Abstract

Low phosphate content in the soil causes insufficient plant needs. Meanwhile, endophytic fungi in nutgrass have great potential as a phosphate solvent and produce indole-3-acetic acid (IAA). Therefore, this study aims to determine the solubilizing phosphate and IAA production by the endophytic fungi of nutgrass and identify the isolates based on rDNA-ITS sequences. Endophytic fungi isolates were cultured in 10 ml of Pikovskaya broth media with Ca5(PO4)3 as the inorganic phosphate source. The PCR results were analyzed using 1.5% agarose gel electrophoresis followed by sequence analysis. The isolation and purification results showed five isolates coded URT1, URT2, URT3, URT4, and URT5, while the solubilizing phosphate levels ranged from 54.03-87.83 ppm, with the highest levels found in the URT4 isolate. Furthermore, the IAA levels ranged from 5.58-45.50 ppm, with the highest levels produced by the URT1 isolate. The molecular analysis with rDNA-ITS sequences showed that URT4 had 97.42% similarity to Aspergillus terreus species, while UTR1 had 100% similarity to Fusarium oxysporum species. Based on the results, the endophytic fungi of nutgrass from A. terreus and F. oxysporum species have great potential as biofertilizers due to the high solubilizing phosphate and IAA levels.
Antibacterial activity test on ethanol extract fraction of Kirinyuh (Chromolaena odorata L.) leaves for multi-drug resistant organisms bacteria Endang Sulistyarini Gultom; Tri Hartanti; Hasnaul Maritsa; Eko Prasetya
Biogenesis: Jurnal Ilmiah Biologi Vol 9 No 1 (2021)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v9i1.17067

Abstract

The resistance of pathogenic bacteria to antibiotics is increasing due to antibiotics with incorrect doses, wrong diagnostics, and the wrong target. Bacteria that have been resistant to several antibiotics are called multi-drug resistant organisms (MDRO) bacteria. Bacterial resistance to some antibiotics requires alternative herbal treatments, one of which is the Chromolaena odorata L. Research must therefore be conducted on the antibacterial activity of the ethanol extract fraction of C. odorata L. leaves for MDRO bacteria, such as Staphylococcus lugdunensis methicillin-resistant Staphylococcus aureus (MRSA), Pseudomonas aeruginosa extended-spectrum beta-lactamase (ESBL), and Klebsiella pneumoniae ESBL. This study aims to determine the antibacterial activity of the ethanol extract of Kirinyuh leaves (C. odorata L.) and the antibacterial activity of the fractionation against MDRO bacteria. Test results of kirinyuh leaf ethanol extract for S. lugdunensis MRSA, P. aeruginosa ESBL, and K. pneumoniae ESBL bacteria each resulted in an inhibition zone with an average diameter of 11.6 mm (strong), 11.5 mm (strong), and 11.13 mm (strong), respectively. Testing the antibacterial activity of the ethanol fraction against MDRO bacteria can show antibacterial activity against all tested bacteria, namely Fraction 5. The results of the antibacterial activity of fraction 5 against K. pneumoniae ESBL, P. aeruginosa ESBL, and S. lugdunensis MRSA bacteria with the formation of inhibition zones formed of 10.2 mm (strong), 8.8 mm (moderate), and 7.9 mm (moderate), respectively. The results of thin-layer chromatography showed that the secondary metabolites contained in the fifth fraction were terpenoids, steroids, and flavonoids.
The Effective Role of Soil Indigenous Fungi on 2.4-D Herbicide Degradation Abdulridha Taha Sarhan
Biogenesis: Jurnal Ilmiah Biologi Vol 8 No 2 (2020)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v8i2.16802

Abstract

The normal field soil environment safeguarded, via indigenous microbes in a native manner, with the aim of turning herbicide waste into productive bio-resources, through fungi activities. This study aims to determine the effective role of soil indigenous fungi on 2.4-D herbicide degradation. The research was conducted over a period of six weeks, on Iraqi cereal field. A total of eight fungi species, belonging to six genera, (Aspergillus candidus L. ATCC 1002, A. niger T. ATCC 16888, Curvularia lunata W. B1933, Penicillium sp. L. 1809, Rhizopus stolonifer L. B9770, Stachybotrys atra C. 1837, Trichoderma harzianum R. IOC 3844, and T. lignorum T. Hartz 1872), were isolated from the soil. During the exposure periods, fungal populations were differently affected, upon treatments with herbicide. The applied herbicide treatments showed different effects on growth and development of the isolated fungi. The results showed that, five of the eight fungi species (C. lunata B1933, Penicillium sp. 1809, R. stolonifer B9770, T. harzianum IOC 3844, and T. lignorum Hartz 1872) were greatly enhanced by the treatment process. However, two fungi (S. atra 1837, and A. candidus ATCC 1002) were affected negatively by the herbicide, while one (A. niger ATCC 16888) remained unaffected. Once extracted from the soil of wheat fields in Iraq, the fungus S. atra 1837, was first isolated. The highest inhibitory effect was caused by 2.4-D herbicide, on the toxigenic fungus S. atra, causing its disappearance from the field at the last week of application. The laboratory experiments showed similar herbicide effects on the isolated fungi at low and moderate levels, while those at the high level (800 µg/ml) were toxic. These results showed that the herbicide 2.4-D treatments have substantial effects on microbial population in the field. When applied at recommended field rate, the herbicide causes transient impacts on fungal population growth and biodiversity, with the majority of the organism becoming responsible for 2.4-D mineralization in the soil. Therefore, the use of 2.4-D herbicide does not only control weed population, but it also affects microbial activities, especially indigenous fungi in the soil.
In Vitro Callus Development on Immature Leaf Explants of Liberica Coffee (Coffea liberica L. cv. Liberika Tungkal Komposit) by the Application of 2.4-D and BAP Lizawati Lizawati; Zulkarnain Zulkarnain; Neliyati Neliyati
Biogenesis: Jurnal Ilmiah Biologi Vol 8 No 2 (2020)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v8i2.11769

Abstract

Conventional vegetative propagation is the process in which new plants are grown from a variety of sources, seeds, cuttings and other parts of the plants. Therefore, the conventional vegetative propagation of liberica coffee by cutting or grafting of stems is hampered by the limited number of stem or branches, which can be used as propagating materials. In addition, the tissue culture technique is another method used to propagate liberica coffee. This study aims to investigate an efficient protocol for embryogenic callus development from leaf explants of Coffea liberica cv. Liberika Tungkal Komposit. The explants used are immature leaves of fully opened liberica coffee. The medium used was Murashige and Skoog (MS) composition supplemented with vitamins, 3% sucrose and solidified with 0.7% agar, and the medium pH was adjusted to 5.8 ± 0.1. The experiment was arranged in a factorial randomized block design, and the first factor was 2.4-D (2.0, 3.0, 4.0, and 5.0 ppm) and then the second factor was BAP (0.0, 0.5, and 1.0 ppm). The results showed that the application of 2.4-D and BAP significantly improved the distribution of callus proliferation on cultured explants. The use of 2.0 ppm 2.4-D + 1.0 ppm BAP resulted in the fastest callus proliferation (19 days after culture initiation). In general, the application of different levels of 2.4-D and BAP successfully induced friable with nodular morphology callus on young leaf explant of liberica coffee. It is believe that the callus has embryogenic capacity and will undergo embryogenesis when transferred to a suitable medium composition
Effect of Chlorella vulgaris extract on high sucrose diet-induced diabetes in Drosophila melanogaster Mohamad Agus Salim; Muhammad Subandi; Yeni Yuniarti
Biogenesis: Jurnal Ilmiah Biologi Vol 9 No 1 (2021)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v9i1.18108

Abstract

Microalgae Chlorella vulgaris is a microscopic green alga known to have potential as a source of food and medicine (functional food). However, the pharmacological capacity of these microalgae species against disease due to metabolic disorders is not understood. Therefore, this study aims to evaluate the effect of C. vulgaris extract on animal models of Drosophila melanogaster, which are fed with a high-sucrose diet (HSD). In this study, the wild-type Drosophila used for seven days of observation was divided into four groups. The first group was used as a control without treatment, the second was treated with HSD (30%), the third was treated with C. vulgaris extract (120 g/L), and the fourth group had double treatment (HSD and C. vulgaris extract). Meanwhile, the parameters observed included fecundity, hatchability, hemolymph glucose, and triglyceride levels. The results showed that the C. vulgaris extract was able to reduce the negative effects of administering HSD. In addition, the single and double treatment of C. vulgaris extract and HSD significantly decreased the levels of hemolymph glucose as well as triglycerides of Drosophila. At the same time, the fecundity parameters and hatchability significantly increased compared to Drosophila that received only HSD treatment. Based on these results, C. vulgaris extract the has potential to be used as an antihyperglycemic agent. However, further study is recommended to prove it.
Literary and Analytical Study of the Source Plants of Kshara (Alkali) for Ksharasutra Preparation in the Management of Fistula-in-ano G.V.P. Samaranayake; W.A.L.C. Walivita; K.M. Chandimal
Biogenesis: Jurnal Ilmiah Biologi Vol 8 No 2 (2020)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v8i2.17348

Abstract

The different surgical techniques and importance in disease treatment were explored by Sushruta, the father of surgery. Furthermore, Anushastra or parasurgical substances and methods from Ayurveda are distinctive in surgical fields as discovered by Sushruta. Kshara or alkali are useful for performing excision/removal, incision/cutting, scraping, alleviating three Doshas and several special procedures, and therefore superior to Shastra (sharp instruments) or Anu Shastra (accessory apparatuses). Also, Kshara is derived from diverse Ayurveda plants and applied in different ways to manage various diseases, including bhagandara (fistula-in-ano) and other numerous anorectal conditions. This study implemented a literary analysis of the plant sources employed to prepare Kshara sutra. There are 23 Kshara source plants provided by Sushruta. The specimens were identified by a comparison of the description to previously published Kshara sutra references. The data recorded encompassed botanical name, local label, location, pharmacodynamics, morphology characteristics, action, chemical constituents, and antimicrobial activity. There are three source plants provided by Sushruta to prepare Kshara sutra for fistula-in-ano management in this study including Curcuma longa Linn., Commiphora mukul, and Euphorbia antiquorum. It is possible to become a reference for Kshara sutra, a novel drug delivery system in Ayurvedic surgery for anorectal diseases and involves a thread smeared with kshara (alkali) applied to induce both mechanical and chemical cutting and healing.

Page 23 of 34 | Total Record : 332

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