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Jurnal Ilmu Ternak Veteriner

JITV
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Published by Indonesian Center for Animal Research and Development

ISSN : 08537380 EISSN : 2252696X DOI : -

Core Subject : Health,

Veterinary

Aims JITV (Jurnal Ilmu ternak dan Veteriner) or Indonesian Journal of Animal and Veterinary Sciences (IJAVS) aims to publish original research results and reviews on farm tropical animals such as cattle, buffaloes, sheep, goats, pigs, horses, poultry, as well as non domesticated Indonesian endemic animals, such as deers, anoa, babirusa, etc. Scope Indonesian Journal of Animal and Veterinary Sciences . The journal will consider primary research papers from any source if they make an original contribution to the experimental or theoretical understanding and application of theories and methodologies of some aspects of animal science and veterinary

Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Hewan dan Zoologi

Articles 1,756 Documents

102 103 104 105 106

Detection of antibody responses by using haemagglutination inhibiton test and the protection titer of avian influenza virus H5N1 subtype Risa Indriani; N.L.P.I Dharmayanti; A Wiyono; Darminto .; L Parede
Jurnal Ilmu Ternak dan Veteriner Vol 9, No 3 (2004): SEPTEMBER 2004
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Study on the detection of antibody responses using haemagglutination inhibition (HI) test and the protection titer to Avian influenza (AI) virus H5N1 subtype local isolate has been conducted at the Research Institute for Veterinary Science (RIVS). A total number of 50 village chicken (10 chicken served as un-injected controls) and 30 quail were injected intramuscularly with inactivated virus of AI H5N1 subtype local isolate. Serum samples were collected 3 weeks after injection and were tested using haemagglutination inhibition tests. The correlation between antibody titer and its protection to AI virus H5N1 local isolate were measured by challenging the birds with AI virus H5N1 local isolate The HI test was then used to determine field serum samples. A total number of 48 village chicken from three (3) Districts (Bekasi, Tangerang and Bogor) and 96 quails from two (2) farms in District of Sukabumi which were all vaccinated with commercial AI adjuvant vaccine were sampled. The study revealed that village chicken and quails showed antibody responses after 3 weeks vaccination and that titer of ≥ 3 log 2 was able to protect chicken and quails when they were challenged with local isolate virus. Based on this result, village chicken field samples from Districts of Tangerang, Bekasi and Bogor showed antibody titer which will protect 50, 100 and 85% of the flocks respectively. While quail field samples from Farm I and Farm II in District of Sukabumi showed antibody titer which will protect 60-100% and 0-80% of the flocks respectively. It is concluded that the study has successfully measured antibody titer to AI virus H5N1 subtype which protect village chicken and quails from local isolate virus challenge so that the results will be used to analyze field serum samples after vaccination program to eradicate AI from Indonesia. Key words: Antibody responses, haemagglutination inhibition test, protection titer, AI virus H5N1subtype

Effect of rice straw silage treated with rumen microbes of buffalo on digestibility and ecosystem of cattle rumen Thalib A; J Bestari; Y Widiawati; H Hamid; D Suherman
Jurnal Ilmu Ternak dan Veteriner Vol 5, No 1 (2000): MARCH 2000
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Treatment of rice straw silage with addition of buffalo rumen microbes was conducted to improve the ruminal digestion of rice straw in ongole cattle. Three fistulated cattles were each introduced to dietary treatment: I. Untreated rice straw (JPTP), II. Rice straw ensilaged with buffalo rumen microbes (SJPMR-Kr), and ID. Elephant grass (RG). All diets were formulated isonitrogeneous (14% crude protein) and fed to animals over a period of 4 weeks. After 4 weeks of feeding trial, rwnen fluid of the animals were evaluated to digest its own basal diet (as substrate). The results show that cumulative gas production resulting from the substrate fermented (96 hours) by rumen fluid from cattle fed diet II is 205% of the diet I and 151 % of the diet ID. Measurements of DMD of the substrates after the gas production procedure show the similar trend (ie. DM digestibilities for JPTP= 33%; SJPMR-Kr= 54% dan RG= 45%). Means of in sacco DMD (72 hours incubation) confirm the results of gas production (ie. in sacco DM Digestibilities for JPTP= 35%; SJPMR-Kr= 44% and RG= 39%). All results described between treatments are highly significant different (P<O.OI). Measurements of rumen ecosystems did not show differences between treated animals (P>0.05), except for total VFA (ie. JPTP= 0.52 mg Inri; SJPMR-Kr= 3,37 mg Inri and RG= 3.15 mg Inri). Key words: Rice straw, silage, microbes, cattle and buffalo

Rescuing genetic material of unexpectedly die animal Syahruddin Said; Takdir Saili
Jurnal Ilmu Ternak dan Veteriner Vol 12, No 2 (2007): JUNE 2007
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Rat cauda epididymides were kept in 1.5-ml centrifuge tubes containing 1 ml milli-Q water or physiological saline (0.9% NaCl) and stored and freezed at -196ºC without cryoprotectant for up to 7 days. After thawing of the cauda epididymis, all spermatozoa were non-motile immediately after collection. All oocytes injected with sperm heads (nuclei) of spermatozoa collected from frozen-thawed cauda epididymis in saline were activated (100%) and gradually decreased (P<0.05) in cauda epididymis frozen in milli-Q water at -196°C (86%), and in control (69%). In activated oocytes, a large proportion of sperm heads had transformed into male pronuclear formation (66-78%). When 1-cell embryos were cultured for 120 h, 7% developed to blastocyst stages. These results indicate that genetic materials of species (at least in the rat) that had unexpectedly die can be saved by a simple method. Key words: ICSI, Sperm Heads, Piezo-Injection, Frozen, Rat

Synthesis of an oxytetracyline-tolidin-BSA immunogen and antibodies production of anti-oxytetracyline developed for oxytetracyline residue detection with enzyme-linked immunosorbent assays technique Widiastuti R; Murdiati TB; Riandari D
Jurnal Ilmu Ternak dan Veteriner Vol 18, No 2 (2013): JUNE 2013
Publisher : Indonesian Center for Animal Research and Development (ICARD)

An oxytetracycline-tolidin-bovine serum albumin (OTC-tolidin-BSA)-conjugate was synthezed as immunogen for producing specific antibodies in immunized rabbits that would be used as reagent for development of OTC residue detection with enzym-linked immunoassays technique. The immunogen was prepared through diazotization tolidin and subsequently reacted with OTC. The red purple immunogen of OTC-tolidin-BSA absorbed at wave lengths of 277 nm and 488 nm under UV screening absorbances and confirmation with the high performance liquid chromatography (HPLC) showed the absence of peak at retention time of 3.46 minutes. Characaterized result with SDS-PAGE showed the molecular weight of the OTC-tolidin-BSA at 69.79 kDA. Subsequently, the immunogen was immunized into New Zealand rabbits in order to produce the polyclonal antibodies. The antibodies were purified using a protein A sepharose column. The OD optimum responses of 0.92 to 1.20 were obtained from the second fractionation at dilution of 1/1000 by titrating the antibodies and OTC-tolidin-BSA coating antigen at concentration of 10 µg/mL on several bleeding times. Key Words: Oxytetracycline, OTC-Tolidin-BSA, Immunogen, Immunization, Antibodies

Genetic diversity and phylogenetic relationship of Indonesian Local goats using microsatellite DNA markers M Syamsul Arifin Zein; S Sulandari; Muladno .; Subandriyo .; Riwantoro .
Jurnal Ilmu Ternak dan Veteriner Vol 17, No 1 (2012): MARCH 2012
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Genetic diversity is important information in the process of conservation and sustainable utilization of animal genetic resources. Thirteen microsatellite markers were used to estimate the degree of genetic diversity on five Indonesian local goats. Results showed the highest average allele diversity present in the locus MAF70 (5.6 ± 2.9), and the lowest was in the locus MAF035 (1.6 ± 0.6), the average number of alleles per locus was 6 ± 2.3. The lowest average alleles diversity present was in the Gembrong goat (2.2 ± 1.1) and the highest was in the Jawarandu goat (4.9 ± 2.2). There is a unique alleles at loci MCM527 and present in all Indonesian local goat with the highest allele frequency on Peranakan Etawa (37.2%) and lowest in Gembrong goat (7.9%). H0 ranged from 0.372 ± 0.173 (Gembrong) to 0.540 ± 0.204 (Peranakan Etawa), and HE ranging from 0.249 ± 0.196 (Gembrong) to 0.540 ± 0.212 (Peranakan Etawa).The genetic differentiation for inbreeding among population (FIS), within population (FIT), and average genetic differention (FST) were 0,0208 (2,08%), 0,1532 (15,32%), and 0,1352 (13,52%), respectively. Locus ILSTS029, BMS1494, MAF035 and INRA0132 had a low PIC value (PIC < 0.25), locus SRCRSP3, OARFCB20, ILSTS005, SPS113, MCM527, and ETH10 provide moderate (PIC = 0.25 to 0.5), and locus CSRD247, MAF70, and ILSTS11 had high value PIC > 0.5. Phylogenetic relationship was consistent with the history of its development based on Kacang goat except was for Gembrong Goat. This research information can be used for conservation strategies and breeding programs on each population of Indonesian local goat. Key Words: Local Goat, Indonesia, Microsatellites, Genetic Diversity, PIC

Bacteriologica acteriological examinations of ducklings, duck's eggs, feed, and rice bran collected from Alibio market, South Kalimantan Istiana .; Suryana .
Jurnal Ilmu Ternak dan Veteriner Vol 2, No 3 (1997)
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Bakteriological examinations of samples consisted of ducklings, duck's eggs feed and rice bran collected from Alabio market, South Kalimantan were conducted in the laboratory during the year 1992 and 1993 . Result of the examinations showed that 22 isolates of Salmonella Hadar, were isolated from 180 internal organ of ducklings (12 .2%), while 1 isolate of Salmonella Typhimurium (0 .5%), and 1 isolate of Salmonella Virchow (0 .5%) was isolated respectively from 180 duck's eggs. Besides, other bacteria were also isolated namely Escherichia coli, Pseudomonas sp., Klebsiella sp ., Citrobacter sp . Enterobacter sp, Proteus sp., and Serratia sp . No pathogenic bacteria were isolated from feed as well as from rice bran . Keywords : Alabio ducklings, duck's eggs, feed, rice bran, Salmonella spp ., South Kalimantan

Exogenous progesterone treatment during pregnancy for increasing milk production and growth of kids of Etawa grade goat I-Ketut Sutama; I-G.M Budiarsana; Supriyati .; Hastono .
Jurnal Ilmu Ternak dan Veteriner Vol 17, No 2 (2012): JUNE 2012
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Naturally, progesterone in ruminant is mainly produced by corpus luteum and it is reponsible for maintaining pregnancy, and affecting udder development and milk production. Exogenous progesterone treatment is expected to give similar positive effect on milk production as the endogenous progesterone does. Fourty mature Etawa grade (PE) does were synchronized for oestrus using Controlled Internal Drug Release (CIDR) followed by natural mating. Does then were treated with CIDR intravaginally, as a source of exogenous progesterone, at day 15-75 after mating (Group A), day 75-135 after mating (Group B), and without exogenous progesterone treatment (Control). Blood samples were taken before and after exogenous progesterone treatment for determination of progesterone concentrations. Soon after birth, kids were separated from their respective dams and they were milk fed through milk bottle. Feed consumption and milk production were measured daily, while bodyweight was measured forthnightly. Results showed that response of does following exogenous progesterone treatment was not expressed by an increase in progesterone concentration in the blood plasm. However, treatment group showed kid with birthweight of 10-13% higher compared to Control Group. In relation to milk yield, birthweight and pre-weaning growth of kids, the positive responses of exogenous progesterone treatment were not significant, though there was a substantial decrease (71.2%) in pre-weaning kid’s mortality. Key Words: Oestrous Synchronization, Progesterone, Milk, Etawa Goat

Nematodiasis in sheep and goats kept under traditional farming practice in Batujajar Beriajaya .
Jurnal Ilmu Ternak dan Veteriner Vol 10, No 3 (2005): SEPTEMBER 2005
Publisher : Indonesian Center for Animal Research and Development (ICARD)

This study was conducted to determine the relation of age, sex, season and reproduction on gastrointestinal nematode parasitism of the two most commonly kept breeds of sheep and goat in Bogor district. A total of 119 Indonesian Thin Tail (ITT) sheep and 130 Peranakan Etawah (PE) goats with different age and sex were monitored for 16 months. Age of sheep and goat was divided into 3 groups respectively, i.e. before weaning (<4 months) 35 and 32, after weaning (4-8 months) 53 and 63; and adults (>8 months) 31 and 35. Each 4 weeks, individual faeces were collected and individual animals were weighed. Information on the mortality, morbidity, pregnancy, slaughtered and sold was recorded. The results showed that Haemonchus contortus and Trichostrongylus spp. were dominant species of nematodes found in the faecal cultured. In the period of monitoring, egg counts of nematodes in sheep were higher (P<0.05) than in goats, however both animals have similar pattern of egg counts. In the first three months, the egg counts remained steady relatively in 3 groups of age, but soon after that the egg counts increased and reached its peak (in March) of 6186 eggs in sheep and 3434 in goats, there after they decreased along with the onset of dry season. A part from this, the egg counts increased (P<0.05) three months before lambing and remained steady until 2 months after partus in sheep, but not in goats. During the monitoring period, weight gain in wet season was lower (P<0.05) as compared to dry season. There was no effect of sex on faecal egg count in either sheep or goats although male sheep had higher egg counts than female sheep had in November and January. Evidence of diarrhoeic faeces was higher in wet season than in dry season. Key Words: Haemonchus contortus, Trichostronglysus spp., Sheep, Goats

Genetic diversity of native chicken based on analysis of D-Loop mtDNA marker Tike Sartika; D Duryadi; S.S Mansjoer; B Gunawan
Jurnal Ilmu Ternak dan Veteriner Vol 5, No 2 (2000): JUNE 2000
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Production was carried out using control region/D-loop mtDNA marker. The base population of native chicken was selected from subpopulation at Cianjur, Jatiwangi, Depok, Bogor I, and Bogor 2. Samples from each population was 10 heads and 2 samples Green Jungle Fowl (Gallus various) from East Java as out Group samples. Two primers binding conserved tRNA Phenylalanine gene and tRNA Glutamine gene were DNA Heavy stranded HI255 (5'-CATCTTGGCATCTTCAGTGCC-3') and DNA Light stranded Ll6750 (5'-AGGACTACGGCTTGAAAAGC-3') was used to amplify D-Ioop mtDNA chicken. PCR-RFLP methods with 6 restriction enzymes 4 cutter such as, Alul (AG↓CT), Hpall (C↓CGG), Mbol (↓GATC), Rsal (GT↓AC), NlaIII (CATG↓) and HaeIII (GG↓CC) were used to detect polymorphism within and between subpopulation. Result of experiment show that mtDNA which was amplified by PCR was 1320 bp, consist of 1227 bp control region/D-loop, 45 bp tRNA Glutamine gene and 48 bp tRNA Phenylalananine gene. PCR product which were digested from 6 endonucleases enzyme show that native chicken within and between population was monomorphic and if its compare with Green Jungle Fowl was polymorphic. Key words: Native chicken, genetic diversity, mtDNA

Residue analysis of sulfametazine in poultry product and its prevention Yulvian Sani; Raphaella Widiastuti
Jurnal Ilmu Ternak dan Veteriner Vol 13, No 3 (2008): SEPTEMBER 2008
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Sulfamethazine (SMZ) is a sulfonamide preparate widely used in feed to control and prevent diseases, and to promote growth. The use of sulfonamides may lead to residue formation, induce microbial resistance and suspected as a carcinogen. A serial study has been undertaken to investigate the withdrawal pattern of SMZ in meat and liver tissues in order to reduce residue of SMZ. A total of 80 day old chicken were divided into 4 groups consisting: (1) negative control without SMZ; (2) positive control dosing with SMZ for 35 days; (3) treatment-1 was dosed with SMZ for 28 days consecutively three times per week then ceased thereafter; and (4) treatment-2 was dosed with SMZ for 30 days consecutively three times per week then ceased thereafter. A field study revealed that some antimicrobials were detected in poultry meat samples, such as sulfamerazine ( = 2.52 ppb or nd – 12.62 ppb) and sulfamethazine ( = 0.02 ppb or nd – 0.09 ppb). An intragastric dosing of SMZ at 50 mg/kgBB did not affected body weight growth. Both positive control and treated groups showed haemorrhagic enteritis, nottling of capsular surface of liver and pale kidneys. Pathological changes were not found in negative control. Microscopically, pathological changes in liver, intestines and kidneys were found consistently in SMZ treatment. Withdrawal time of SMZ in broilers was between 5 to 10 days. Therefore to produce safe and healthy poultry products, sulfonamides treatment should be withdrawn 5 to 10 days prior to culling of birds and substitution of feed to unmedicated feed within this period is strongly recommended. Key Words: Residue, Sulfametazine, Poultry, Meat, Prevention

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Ahmadi Riyanto

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ahmadi_puslitbangnak@yahoo.com

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Location
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Home Page

OAI Link

Editorial Team

Contact

Reviewer

Google Scholar

Contact Name Ahmadi Riyanto

Contact Email medpub@litbang.deptan.go.id

Phone -

Journal Mail Official ahmadi_puslitbangnak@yahoo.com

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Location Kota bogor, Jawa barat INDONESIA

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Contact Name
Ahmadi Riyanto

Contact Email
medpub@litbang.deptan.go.id

Phone
-

Journal Mail Official
ahmadi_puslitbangnak@yahoo.com

Editorial Address
-

Location
Kota bogor,

Jawa barat

INDONESIA