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Jurnal Ilmu Ternak Veteriner

JITV
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Published by Indonesian Center for Animal Research and Development

ISSN : 08537380 EISSN : 2252696X DOI : -

Core Subject : Health,

Veterinary

Aims JITV (Jurnal Ilmu ternak dan Veteriner) or Indonesian Journal of Animal and Veterinary Sciences (IJAVS) aims to publish original research results and reviews on farm tropical animals such as cattle, buffaloes, sheep, goats, pigs, horses, poultry, as well as non domesticated Indonesian endemic animals, such as deers, anoa, babirusa, etc. Scope Indonesian Journal of Animal and Veterinary Sciences . The journal will consider primary research papers from any source if they make an original contribution to the experimental or theoretical understanding and application of theories and methodologies of some aspects of animal science and veterinary

Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Hewan dan Zoologi

Articles 1,756 Documents

101 102 103 104 105

Spontaneous cholangiohepatitis in broiler chickens: immunohistochemical study of Ito cells E Handharyani; K Ochiai; W Winarsih; E Harlina; Salfina Nurdin Estuninngsih
Jurnal Ilmu Ternak dan Veteriner Vol 6, No 4 (2001): DECEMBER 2001
Publisher : Indonesian Center for Animal Research and Development (ICARD)

The function of Ito cells is expanding from a fat-storing site to a center of extracellular matrix metabolism and mediator production in the liver. Immunohistochemical reactivities of Ito cells were examined in eight livers of broiler chickens affected with spontaneous cholangiohepatitis and six chicken livers with malformation of extrahepatic biliary tracts. The livers in both groups revealed severe diffuse fibrosis. Ito cells expressing HHF35 muscle actin and desmin actively proliferated in the fibrotic foci of the all livers. The immunoreactivities of Ito cells to antibodies were enhanced compared with those in normal livers. There were no immunohistochemical differences between the Ito cells of two groups. From these findings, it was suggested that Ito cells actively proliferate and show enhanced immunoreactivities in the livers affected with cholangiohepatitis andmalformation of extrahepatic biliary tracts. Key words : Cholangiohepatitis, immunohistochemistry, ito cell, broiler chicken

A study on the pathogenesis of Trypanosoma evansi in buffaloes, Holstein Friesian and Ongole cattle S. Partoutomo; M. Soleh; F. Politedy; A. Day; A.J. Wilson; D.B. Copeman
Jurnal Ilmu Ternak dan Veteriner Vol 1, No 1 (1995)
Publisher : Indonesian Center for Animal Research and Development (ICARD)

A study on the pathogenesis of Trypanosoma evawi was carried out in 5 buffalo calves and 5 buffalo adults, 6 Holstein-Friesian calves and 6 Holstein-Friesian adults, and 6 Ongole calves and 6 Ongole adults, each ofwhich was divided into 3 infected and 2 uninfected buffalo calves and adults, and 3 infected and 3 uninfected calves and adults of Holstein Friesians and Ongoles. None of infected animals showed acute clinical signs along the course of the observation period, however roughness of the hair and skin, emaciation, weakness and loss of weight gains were the common clinical signs. Clinical signs of calves were more severe than adults, and those of buffaloes were more severe than cattle . Gross pathological changes were not specific . The mortality rate was 2/3 in buffalo calves, 1/3 in Holstein-Friesian calves and 1/3 in Ongole calves . None of infected adults died of infection. Buffaloes had longer and higher parasitemia than Holstein-Friesians or Ongoles. Erythrocyte counts of infected animals decreased to lower levels than controls, however they fluctuated in the normal values . Haemoglobin and PCV values of infected animals were significantly lower than those of non-infected controls, and those of calves were more severe than adults, and those of buffaloes were more severe than cattle . Infections resulted in loss of weight gains which was the greatest in buffaloes then followed by Holstein-Frisians and finally Ongoles.

Comparison of serological test for toxoplasmosis using Immunostick Assay, ELISA and Latex Agglutination Test Didik T. Subekti; E. Kusumaningtyas
Jurnal Ilmu Ternak dan Veteriner Vol 16, No 3 (2011): SEPTEMBER 2011
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Many serological methods were developed in order to improve detection and identification of an infectious diseases. ELISA (enzyme linked immunosorbent assay), is a well-known as the most serological technique and widely used for infectious disease diagnoses, especialy toxoplasmosis in human and animal. Unfortunately, the technique requires specific tools and expertise that are not always available in the field. Inn addition, ELISA needs longer process than other techniques for both small and large number of samples. Another serological assay, namely Latex Agglutination Test (LAT) was known as populer and mostly applied in field for toxoplasmosis diagnosis in Indonesia, both for human and animal serum. However, the accuracy of this technique was slightly lower compared to ELISA, especially for weakly seropositive. Recently, an immunostick assay technique was developed as alternative serological method which has high accuracy as ELISA. The immunostick assay was rapid and simple like latex agglutination test. The immunostick assay was evaluated to detect Toxoplasma gondii infection in goat serum samples and compared to ELISA and LAT. The immunostick assay had a true agreement 95.88% - 100% againts ELISA and their strength of agreement was very good aggreement with ELISA (k = 0.796; AC1 = 0.948). However, LAT had true agreement only 75.74%, either with ELISA or immunostick assay. The strength of agreement of LAT was moderately aggreement with ELISA or immunostick assay (k = 0.447; AC1 = 0.582). Therefore, the immunostick assay could become the first choice for rapid serological assay on toxoplasmosis diagnosis, both in the laboratory and in the field. Key Words: Latex Agglutination, ELISA, Immunostick Assay, FELISA, Toxoplasmosis

Effectivity of various concentration of lactose in Tris extender on liquid semen viability of Saanen bucks Surya Natal Tambing; I-K Sutama; R.I Arifiantini
Jurnal Ilmu Ternak dan Veteriner Vol 8, No 2 (2003): JUNE 2003
Publisher : Indonesian Center for Animal Research and Development (ICARD)

The objective of this study is to determine the best concentration of lactose in Tris extender in maintaining liquid semen viability of Saanen bucks stored at 5°C. Four heads of Saanen bucks of 2-4 years old were used as semen source. Semen was collected once a week using an artificial vagina. Variance analysis was conducted based on completely randomized design with three treatments, i.e 30, 60, and 90 mM lactose (L30, L68 and L90). Duncan test was used to observe the different between treatment. Results indicated that the mean percentage of motility, live sperm, intact plasma membrane, and intact acrosomal cap of liquid semen in treatment L30 (70.83; 78.20; 79.56 and 81.20% respectively) were significantly higher (P<0.05) than treatment L60 (67.08; 73.73; 75.01 and 76.87% respectively) and treatment L90 (61.50; 71.88; 69.08 and 71.33% respectively). After storage for 48 hours at 5°C apparently the mean percentage of motility, live sperm, intact plasma membrane and intact acrosomal cap in treatment L30 were significantly higher (P<0.05) than those of in treatment L60 and L90. Decreasing percentage of motility, live sperm, intact plasma membrane, and intact acrosomal cap during storage at 50C from 0 until 48 hour in treatment L30 (42.83; 19.75; 40.84 and 39.00% respectively) were significantly lower (P<0.05) than those of in treatment L60 (55.41; 23.06; 50.14; and 50.90% respectively) and treatment L90 (54.83; 29.93; 50.14 and 50.97% respectively). It was concluded that supplementation 30 mM lactose in Tris extender could maintain liquid semen viability in Saanen bucks. Key words: Saanen bucks, lactose, liquid semen

Curcumin effect on Bleomycin-induced pulmonary fibrosis in Mus musculus Annisa Rahmi; A. Setiyono; V. Juniantito
Jurnal Ilmu Ternak dan Veteriner Vol 20, No 2 (2015): JUNE 2015
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Curcumin, a curcuminoid compound of turmeric has been demonstrated to have anti-oxidant and anti-inflammatory properties. Bleomycin (BLM) is an anti-cancer drug induced pulmonary fibrosis in human and animals. This study was aimed to investigate biological effects of curcumin on bleomycin-induced pulmonary fibrosis in mice (Mus musculus) through pathomorphological assessment. In this study, 16 mice ddy strain were divided into four groups, namely (i) control, mice were subcutaneously (SC) injected with 100 µl sterilized aquadest in dorsal skin, (ii) BLM group, injected SC with 100 µl of 1 mg/ml BLM in dorsal skin, (iii) Curcumin (CMN) group, mice were intraperitoneally (IP) injected with 100 mg/kg body weight (BW) curcumin dissolved in 0,5% carboxy methyl cellulose (CMC) and injected with 100 µl sterilized aquadest SC, (iv) BLM+CMN group, injected SC with 100 µl of BLM 1 mg/ml and injected IP with 100 mg/kg BW CMN in 0,5% CMC. All treatments were performed daily for four weeks period. The lung samples were collected and fixed in buffered neutral formalin (BNF) 10%. Histopathological evaluation was performed with hematoxylin-eosin (HE) and Masson’s trichrome (MT) stains. The results showed that BLM treatment significantly increased fibrosis area and alveolar wall area fraction as compared to control. OIn the other hand, CMN treatment significantly reduced fibrosis area and alveolar wall area fraction in mice treated with BLM. In conclusion, our study showed that CMN treatment may inhibit lung fibrogenesis in BLM-induced pulmonary fibrosis.

Characterisation of protein antigen from Fasciola gigantica of different age S Endah Estuningsih; S Widjajanti
Jurnal Ilmu Ternak dan Veteriner Vol 4, No 1 (1999): MARCH 1999
Publisher : Indonesian Center for Animal Research and Development (ICARD)

The protein antigens extracted from adult fluke Fasciola gigantica, 3, 6 and 9 weeks old and newly excysted juvenile (NEJ) were identified using SDS-PAGE and immunoblotting techniques. Sera from fat-tailed sheep which artificially infected with the metacercariae of F. gigantica were used for immunoblotting. The results showed that the protein antigen profile of adult fluke, 6 and 9 weeks old flukes had similar. Bands with molecular weight between 24 kDa to 114 kDa. Protein bands with molecular weight <24 kDa and >198 kDa were also detected from the adult fluke. The use of immunoblotting technique, there were two antigenic protein molecules identified from adult fluke, NEJ and 3, 6, and 9 weeks old fluke with the molecular weight 46 kDa and 47 kDa. The protein band with molecular weight >198 kDa shown thicker on the NEJ than that of adult fluke, and 6 and 9 weeks old flukes. The role of protein with molecular weight of 46 and 47 kDa were the interested findings need to be evaluatedfor serological analysis. Key words : Protein antigen, Fasciola gigantica, SDS-PAGE, immunoblotting

Phage typing and sensitivity test to antibiotics of Salmonella enteritidis isolates from Indonesia Sri Poernomo; Adin Priadi; Lily Natalia
Jurnal Ilmu Ternak dan Veteriner Vol 11, No 2 (2006): JUNE 2006
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Salmonella enteritidis (SE) is frequently implicated in disease outbreaks such as human food poisoning. Phage typing have been proved to be a valuable and sensitive tool in the control of SE infections. The ability of phage to distinguish varieties among apparently identical serotypes led to the development and acceptance of phage typing as a significant epidemiological procedure. To determine the epidemiological pattern of SE, phage typing of 53 SE isolated from various sources in Indonesia during 1991–1999, has been conducted using 16 typing phages of phage typing scheme of SE obtained from the International Collaborating Center for Enteric Phage typing, Central Public Health Laboratory, Colindale, UK. The lyse blood isosensitest was then used to test the sensitivity of the Salmonella isolates to antibiotics. The phage typing results obtained that of 53 Salmonella isolates there were one S. infantis, one S. berta, and 46 SE phage type 4, 2 SE phage type 7 (from chicken and water), 1 SE phage type 6 (from chicken) and 2 SE phage type 1 (from chicken). SE phage type 4 isolates comprised of 2 isolates from human, 19 isolates from chicken (young and adult), 17 isolates from day old chicks, 4 isolates from fluff, 2 isolates from chicken meat, 1 isolate from poultry farm water, 1 isolate from dog organ. These findings indicated that contaminated chicken appeared to be the sources of human and dog for SE infection. The results of sensitivity test of the isolates to antibiotics showed that most of the Salmonella isolates from Indonesia were resistant to the antibiotics tested. Key Words: Salmonella Enteritidis, Phage typing, Sensitivity test, Indonesia, Chicken

Dietary energy and crude protein requirements of Ettawah Cross Kids: 1. Intake, digestibility, availability and utilization of nutrients I-W Mathius; I.B Gaga; I-K Sutama
Jurnal Ilmu Ternak dan Veteriner Vol 7, No 2 (2002): JUNE 2002
Publisher : Indonesian Center for Animal Research and Development (ICARD)

An experiment was designed in order to study the crude protein and energy requirement of Ettawah Cross growing goats. Twenty seven kids (average body weight of 11.80 + 1.4 kg) were used and randomly allotted to a 3 x 3 factorial arrangement of treatments, consisting of three different levels of crude protein and three levels of energy. Results from this experiment showed that animal performance was effected by dietary treatments. Increasing levels of energy ration significantly (P<0.05) decreased the dry matter intake, with overall mean values were 3.0; 2.8 and 2.5% of body weight for low, medium and high levels of energy respectively. Dietary treatments increased energy (EM) and crude protein intake, and overall values were 0.2046 + 0.016 Mkal/kg BW0.75 and 8.20 + 2.473 g/kg BW0.75 repectively. Positive response on animal performance was also effected by dietary treatment, with overall mean value of 86.40 + 29.59 g head-1day-1. The highest response on animal performance (ADG 123.3 g) was found on kids fed diet containing combination of low level of energy and high level of crude protein. Meanwhile, the lowest average daily gain (45 g) was resulted by kids fed ration containing combination of high level of energy and low level of crude protein. Key words: Protein-energy, etawah cross kids, growing fase

Etabolism in compensatory growth . III. The urea, glucose and C02 entry rates in animal undergoing compensatory growth Pram Mahyudin; E Teleni
Jurnal Ilmu Ternak dan Veteriner Vol 2, No 1 (1996)
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Glucose (GER), Urea (UER) and C02 (C02 ER) entry rates were studied at four points in the growth curve viz: before feed restriction (PI) after 8 weeks of feed restriction (P2), after 3 weeks (P3) and 15 weeks (P4) following resumption ofad libitum feeding. Sixteen Merino wethers were used and offerred pelleted lucerne (Medicago sativa) ad libitum for 3 weeks; then they were divided into 2 groups of eight. Group I continued to be fed ad libitum and Group 11 was fed pelleted lucerne at half maintenance level for 8 weeks and then fed ad libitum until the end of experiment. During feed restriction (P2), UER, urinary urea and urea transferred from the blood to the gut were 74% lower in group II than those in group I due to the reduction of N intake . At P2 GER and C02ER were also lower (53% and 56%, respectively) because of the reduction of available glucose precursor and metabolic rate. Similarly AV concentration difference of glucose, glucose taken up by the hind-limb muscle and the percentage of glucose taken up by muscle that was oxidised were reduced by 52%, 86% and 48%, respectively . When animals resumed ad libitum feeding, the components of urea entry rate (except plasma urea concentration), GER and C02ER were markedly increased indicating A switch to the anabolic mode, followed by increased glucose taken up and oxidised by the hind-limb muscle . The significance of glucose in muscle metabolism during compensatory growth was shown in the dramatic increase in the actual rate of glucose oxidation per unit muscle weight . It appears that the priority of usage of glucose taken up by muscle during compensatory growth is for oxidation to both C02 and lactate. Key words: Glucose entry rate, C02 entry rate, plasma urea

Profile of blood glucose and ultrastucture of beta cells pancreatic islet in alloxan compound induced rats I Nyoman Suarsana; B.P Priosoeryanto; M Bintang; T Wresdiyati
Jurnal Ilmu Ternak dan Veteriner Vol 15, No 2 (2010): JUNE 2010
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Diabetes is marked by elevated levels of blood glucose, and progressive changes of the structure of pancreatic islet histopathology. The objective of this research was to analyse the glucose level and histophatological feature in pancreatic islet in alloxan compound induced rats. A total of ten male Spraque Dawley rats of 2 months old were used in this study. The rats were divided into two groups: (1) negative control group (K-), and (2) positif induced alloxan group (diabetic group =DM). The rats were induced by a single dose intraperitonial injection of alloxan compound 120 mg/kg of body weight. The treatment was conducted for 28 days. Blood glucose levels of rats were analysed at 0, 4, 7, 14, 21, and 28 days following treatment. At the end of the experiment, rats were sacrificed by cervical dislocation. Pancreas was collected for analysis of histopathological study by Immunohistochemical technique, and ultrastructural study using transmission electron microscope (TEM). The result showed that Langerhans islet of diabetic rat (rat of DM group) showed a marked reduction of size, number of Langerhans islet of diabetic rat decrease, and characterized by hyperglycemic condition. By using TEM, beta cells of DM group showed the rupture of mitochondrial membrane, the lost of cisternal structure of inner membrane of mitocondria, reduction of insulin secretory granules, linkage between cells acinar with free Langerhans islet, and the caryopicnotic of nucleus. Key words: Alloxan, Beta Cells, Rat, Blood Glucose, Immunohistochemistry

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Home Page

OAI Link

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Contact Name Ahmadi Riyanto

Contact Email medpub@litbang.deptan.go.id

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Location Kota bogor, Jawa barat INDONESIA

Abstract

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Abstract

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Abstract

Contact Name
Ahmadi Riyanto

Contact Email
medpub@litbang.deptan.go.id

Phone
-

Journal Mail Official
ahmadi_puslitbangnak@yahoo.com

Editorial Address
-

Location
Kota bogor,

Jawa barat

INDONESIA