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Jurnal Ilmu Ternak Veteriner

JITV
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Published by Indonesian Center for Animal Research and Development

ISSN : 08537380 EISSN : 2252696X DOI : -

Core Subject : Health,

Veterinary

Aims JITV (Jurnal Ilmu ternak dan Veteriner) or Indonesian Journal of Animal and Veterinary Sciences (IJAVS) aims to publish original research results and reviews on farm tropical animals such as cattle, buffaloes, sheep, goats, pigs, horses, poultry, as well as non domesticated Indonesian endemic animals, such as deers, anoa, babirusa, etc. Scope Indonesian Journal of Animal and Veterinary Sciences . The journal will consider primary research papers from any source if they make an original contribution to the experimental or theoretical understanding and application of theories and methodologies of some aspects of animal science and veterinary

Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Hewan dan Zoologi

Articles 1,756 Documents

103 104 105 106 107

Detection of deoxynivalenol and T-2 toxin in feeds by gas chromatography with Electron Captured Detector R. Widiastuti; Indraningsih .
Jurnal Ilmu Ternak dan Veteriner Vol 14, No 3 (2009): SEPTEMBER 2009
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Deoxynivalenol (DON) and T-2 toxin are trichothecene mycotoxins produced by Fusarium spp. may pose to threat animal health. This research was aimed to validate an analysis method for DON and T-2 toxin detection in feed using a gas chromatography with electron captured detector ECD (GC-ECD) and to study the contamination level of DON and T-2 toxin in feed. Samples were extracted with organic solvents and derivitized with DMAP and HFBA prior to the detection with GC. Both toxins can be detected simultaneously with satisfied validation with GC. Analysis for DON showed that 17 out of 24 corn samples were positive (0.450 to 1.126 mg/kg), none was positive for 20 rice husk samples and 1 out of 14 cattle feed samples was positive (0.043 mg/kg), whereas analysis for T-2 toxin showed that 2 out of 24 corn samples were positive (0.022 and 0.063 mg/kg), 14 out of 20 rice husk samples were positive (0.027 to 0.720 mg/kg) and 6 out of 14 cattle feed samples were positive (0.039 to 0.084 mg/kg). The study showed that DON and T-2 toxin can be detected using GC-ECD. Whereas, the analysis results from field samples showed that the feeds were still safe to be used for animal feed, however a routine surveillance is needed to minimize further potential hazards. Key words: Deoxynivalenol, T-2 toxin, Gas Chromatography, Feeds

A rapid, solid phase extraction (SPE) technique for the extraction and gas chromatographic determination lindane pesticide residue in tissue and milk Yuningsih .; Sri Yuliastuti
Jurnal Ilmu Ternak dan Veteriner Vol 10, No 1 (2005): MARCH 2005
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Organochlorine pesticide contamination in feed can cause residue in animal product (tissue and milk), so its become a problem in food safety. Solid phase extraction (SPE) has been carried out for determination organochlorine pesticide residues in food animal production. The technique was rapid, not costly and produce limited amount of hazardous-waste. Samples were homogenized with acetonitrile trough cartridge C18, eluted in fluorocyl column with 2% ether-petroleum or acetonitrile fortissue and milk samples respectively. The recoveries of tissue sample by addition lindane standard solution: 0.50 and 1.00 μg are 85.10 and 103.10% respectively, while that of milk with the addition of 0.50, 1.00 and 1.50 μg are 83.80, 88.69 and 91.24% respectively. Three replicates were carried out for every sample. According of validation criteria of FAO/IAEA the recovery for analysis of pesticide residues was 70-110%. Therefore, the method is applicable. Key Words: Pesticide, Tissue, Milk, SPE, Gas Chromatography

Latex agglutination test for field diagnosis of haemorrhagic septicaemia Lily Natalia
Jurnal Ilmu Ternak dan Veteriner Vol 6, No 3 (2001): SEPTEMBER 2001
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Pasteurella multocida is bacterial pathogens that cause haemorrhagic septicaemia (HS) in cattle and buffaloes. Various tests have been used to differentiate types of P. multocida, as well as to diagnose this specific disease. A latex agglutination test has been developed for the detection of P. multocida B:2 which is the causal agent of HS. This test is a rapid and simple test suitable for local laboratorium to diagnose HS cases in the field. A heat stable antigen consisting of mainly lipopolysaccharide (LPS) extract of formalin killed P. multocida 0019 was used to produce specific antibody against P. multocida B:2. The antiboy was then used to sensitise latex particles. Latex agglutination test have been used to screen some P. multocida field isolates and this test have been proven to be specific, simple and easy to be used in detecting P. multocida B:2. The specificity is due to antibodies recognising LPS or LPS protein complexes. Sensitised latex was stable at 4° C for at least12 months. This test should be used as an aid to diagnosis and employed principally to confirm and support clinical and post mortem findings of HS. Keywords: Pasteurella multocida B:2, haemorrhagic septicaemia (HS), diagnosis, latex agglutination test

The development of fowl cholera vaccine: II. Pathogenicity and vaccine protection of Pasteurella multocida local isolates in experimental ducks Supar .; Yudi Setiadi; Djaenuri .; Nina Kurniasih; B Poerwadhikarta; Sjafei .
Jurnal Ilmu Ternak dan Veteriner Vol 6, No 2 (2001): JUNE 2001
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Pasteurellosis or fowl cholera in ducks occurs sporadically along the year in many high duck population areas of Java and other parts of Indonesia. Some isolates of Pasteurella multocida from ducks and chicken are kept at the BALITVET culture collection. The aims of this research were to evaluate the pathogenicity of local isolates and imported strains of P. multocida and to study the pasteurellosis local isolate vaccine and protection assay in ducks. Two imported strains of P. multocida (BCC 1359, BCC 1362) and 6 local isolates (BCC 299, BCC 2331, DY1, DY2, 12TG, 15TG) were used in this study. In the pathogenicity assays the imported strains and local isolates were activated in mice and in duct and then in brain hearth infusion broth containing 5% normal sheep serum. Each of broth culture was diluted, each dilution (102 and 104) of strains or isolates was injected intraperitoneally into a group of normal ducks. Antigen for vaccine, each was produced in sheep blood (5%) agar. Cells were harvested and killed with 0.1% formalin. Monovalent, bivalent, and polyvalent vaccines were prepared, at concentration equal to the Macfarland standard tube No 10, and each was adjuvanted with alhydrogel at final concentration of 1.5%. Each vaccine type was injected into a group of 10 week old ducks (8 animals per group), with 0.2 ml/injection. Four weeks later each animal in group were boostered with the same vaccine, dose, route as the previous injection. Before vaccination each animal was bleed through wing vena, then every two weeks, serum was collected and stored at -20oC. Two weeks after boostered, three days after the last blood sample collection, half animal of each group were challenged intraperitonelly with the BCC 2331 and half with DY2 live broth culture. The pathogenicity assays showed the only BCC 2331 and DY2 killed the experimental ducks, the other did not. The animals vaccinated with either BCC 2331, DY2 or bivalent (BCC 2331+DY2) vaccines were protected with either life bacterial challenged of either BCC 2331 or DY2 local isolates. It is likely, P. multocida BCC 2331 and DY2 isolates can be used for pasteurellosis candidate vaccine used in Indonesia, but it still needs more studies in the immunological of protective antigens. Key words: Pasteurella multocida, fowl cholera, ducks, vaccine, protection

Enrofloxacin and ciprofloxacin residues in broiler chicken post administration of enrofloxacin Raphaella Widiastuti
Jurnal Ilmu Ternak dan Veteriner Vol 13, No 2 (2008): JUNE 2008
Publisher : Indonesian Center for Animal Research and Development (ICARD)

The presence of drug residue in animal product may threat human health such as increasing resistance to Camplyobacter infection treatment due to the use of enrofloxacin. The objectives of this research was to study the concentration of enrofloxacin (EFX) and ciprofloxacin (CFX) residues distributed in breast, thigh and liver and to determine the elimination rate of such residues in chicken which recieved enrofloxacin orally at 50 mg/kg bw daily for 9 days consecutively started at 23rd day to 31st day of age. Samples of breast muscle, thigh and liver were collected from chicken at ½ to 288 h after the drug administration. The concentration of EFX and CFX in samples were analyzed by high pressure liquid chromatography (HPLC). The results showed that the highest residues concentration of EFX and CFX at ½ hr post administration of EFX was: in liver (117.47 ng/g), breast (95.16 ng/gr) and thigh (71.40 ng/g), whereas the order of elimination rate was: thigh (120 hr), liver (168 hr) and breast (240 hr). The results also showed that concentration of CFX was higher than that of EFX. Key Words: Enrofloxacin, Ciprofloxacin, Residues, Broiler Chicken

Intake and digestibility of untreated and urea treated rice straw base diet fed to sheep D Yulistiani; J.R Gallagher; R.J. Van Barneveld
Jurnal Ilmu Ternak dan Veteriner Vol 8, No 1 (2003): MARCH 2003
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Rice straw as one of agricultural by-products has low quality due to low content of essensial nutrients like protein, energy, minerals and vitamin as well as poor palatability and digestibility. Therefore, the quality of rice straw needs to be improved in order to increase its utilization by gastrointestinal tract of ruminants. The purpose of this study is to compare untreated and urea treated rice straw as basal diets for sheep. Twelve mature Merino wethers (average body weight 53.62 + 3.44 kg) were separated into 4 groups based on their live weight with each groups assigned three diets, that are: diet 1 untreated rice straw with high forage legume content, diet 2 urea ensiled rice straw and diet 3 rice straw sprayed with urea solution at feeding time. Diets were allocated based on a randomized complete block design. Urea ensiled rice straw was prepared by spraying chopped straw with urea solution to yield straw containing 4% urea and 40% moisture, then kept in air tight polythylene bags for 6 weeks. The untreated, ensiled and urea supplemented rice straw were mixed with other feed ingredients to provide isoenergetic and isonitrogenous diets. Diets were formulated to meet maintenance requirement according to NRC. Sheep were adapted to experimental diets for 15 days, and after adaptation period, a metabolism trial was conducted. Results reveal that dry matter intake permetabolic body weight (DMI/W0.75), DE (digestible energi) intake and apparent digestibility of NDF (neutral detergent fibre) were not significantly different between diet 1 and diet 2. Apparent digestibility of DM (dry matter), OM (organic matter), and ADF (acid detergent fibre), as well as N retention were not significantly different between three diets. Positive result in N retention was only observed in diet 2, while others were negative. It may be concluded from this study that untreated rice straw basal diet supplemented with forage legume offer an alternative method other than urea ensiled for improving the nutritional value of rice straw as a ruminant feed on small farmer. Key words: Rice straw, urea treated, digestibility, and sheep

Performance of EPMp broiler duck fed various levels of lysine during starter period Purba M; Haryati T; Sinurat AP
Jurnal Ilmu Ternak dan Veteriner Vol 20, No 1 (2015): MARCH 2015
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14334/jitv.v20i1.1119

The aim of this study was to determine optimal requirement of lysine of broiler EPMp ducks during starter period. The study was designed in a completely randomized design (CRD) with four dietary treatments, four replications, and each replication consisted of 10 ducks. The treatments were: T1 (ration, with 0.70% digestible lysine); T2 (ration, with 0.85% digestible lysine); T3 (ration, with 1.00% digestible lysine); T4 (ration, with 1.15% digestible lysine). Variables measured were: feed intake, body weight gain and feed conversion ratio (FCR). Results showed that average body weight gain of EPMp broiler ducks was significantly affected (P<0.05) by the level of lysine in the diet, but feed intake and FCR were not significantly (P>0.05) affected. Mean body weight gain of EPMp broiler duck with T4 ration (1.15%) of lysine was significantly higher compared to T3 ration (1.00% of lysine), but between T4 to T1 and T2 treatmeants were not significantly different (P>0.05). T3 treatment compared to T1 and T2 treatments were not significantly different (P>0.05). There is a pattern of decreasing feed consumption and FCR by increasing content of lysine in the diet, protein and lysine consumption during the starter period. It is concluded that administration of digested lysine at 0.70 and 0.85%, protein and metabolized energy respectively by 18% and 2800 kcal/kg EM in feed were considered sufficient to generate performance (feed consumption, body weight gain and FCR) of EPMp broiler ducks in starter period.

Anthelmintic effect of Zingiber purpureum infuse and extract on adult worms of Haemonchus contortus in vitro Beriajaya .; T.B. Murtiadi; Murti Herawaty
Jurnal Ilmu Ternak dan Veteriner Vol 3, No 4 (1998)
Publisher : Indonesian Center for Animal Research and Development (ICARD)

The aim of this study is to determine anthelmintic effect of “bangle” (Zingiber purpureum) on adult worms of Haemonchus contortus in vitro. Bangle were washed and cut into small pieces, and then put into oven with temperature of not more than 50oC for 4 days. Furthermore, it was grained with a mesh of 0.75 mm into a form of powder. Infuse was made by mixing a powder of “bangle” with 100 ml distill water on beaker glass and then heat on water bath for 30 minutes at temperature of 90oC. It was made at a concentration (w/v) of 0%, 2.5%, 5%, 10%, 20% and 40%. Extract was made by percolation with methanol until filtrate produced, and this filtrate was condensed with rotapavor at 48oC with rotated at 200 rpm. Extract were made into a concentration (w/v) of 0%, 0.5%, 1%, 2%, 4% and 8%. Ten adult worms of H. contortus in Petri-dish were dipped with infuse or extract at each concentration with 5 replication. Observations were made every 30 minutes for a period of 6 hours to determine whether the adult worms were still alive or death. Faeces from donor of sheep artificially infected with H. contortus were cultured in vermiculite with infuse or extract used to keep humidity. Each treatment was repeated 5 times. Observations were made at the end of experiment to calculate on the total number infective larvae. The results showed that infuse or extract of “bangle” have anthelmintic effect on H. contortus. The form of extract showed a better effect compared with infuse at a concentration of 0.5% and 2.5% respectively. The increase of concentration will result in a better effect. Key words : Haemonchus contortus, Zingiber purpureum, “bangle”

Effect of ß-xilanase and ß-glucanace supplementations on the performances of broiler chickens I.A.K Bintang; A.P Sinurat; P.P Ketaren
Jurnal Ilmu Ternak dan Veteriner Vol 11, No 2 (2006): JUNE 2006
Publisher : Indonesian Center for Animal Research and Development (ICARD)

A study was conducted to evaluate the effect of enzyme supplementation on the performances of broiler chickens. One hundred and twenty five day old chicks (DOC) were allocated into five dietary treatment with 5 replicates. Every replicate had 5 DOC. The treatments were: control, control + 0.02% ß-xylanase, control + ß glucanase at three levels (0.05; 0.10 and 0.20%). The treatments were carried out in a randomized block design. Parameter measured were: feed intake, live weight gain and feed conversion ratio (FCR) at 3 and 5 weeks old, while carcass, internal organ, abdominal fat, thickness of intestine, and mortality were observed only at 5 weeks old. The results showed that enzyme supplementation did not significantly (P>0.05) affect feed intake and live weight gain of chicks at 3 weeks old, but the FCR of enzyme supplementation was significantly (P<0.05) better as compared with the control. FCR of birds fed with ß-glucanase tended to be better than the ß-xylanase supplementation (P>0.05). The enzyme addition did not significantly (P>0.05) affect all parameters recorded at 5 weeks old chickens. It is concluded that the best treatment for chicks at 3 weeks old was 0.05% ß-glucanace supplementation. This treatment improved 7.55% FCR as compared to the control. Key Word: Enzyme, Performances, Broiler

Using CR1aa versus KSOM as the culture medium for in vitro embryo production of cattle Endang Triwulanningsih; M.R Toelihere; J.J Rutledge; T.L Yusuf; B Purwantara; K Diwyanto
Jurnal Ilmu Ternak dan Veteriner Vol 7, No 1 (2002): MARCH 2002
Publisher : Indonesian Center for Animal Research and Development (ICARD)

This research has been conducted at the laboratory of in vitro fertilization in the Department of Animal Science University of Wisconsin, USA. These embryos can be used for improving genetic value of Indonesian cattle. Oocytes were matured in TCM- 199 medium (in 5% CO2 incubator and at 390C) enriched with follicle stimulating hormone (FSH) 10 μl/ml, oestradiol 17 β 1μl/ml and 10% Fetal Calf Serum (FCS). The oocytes were fertilized in vitro with motile sperm and incubation between sperm and oocytes in fertilization medium Tyroide Albumin Lactate Pyruvate (TALP) for 20 hours. All zygotes were cultured in CR1aa (n=1549) medium versus modification of protein-free pottasium simplex optimized medium (KSOM) (n=675) up to blastocyst stage and were fed FCS 5 μl/50 μl medium on day 6, as treatment A and B respectively. Data were analyzed by completely randomized design with SAS program. Percentages of cleavage, morula, blastocyst, expanded blastocyst, unfertilized and degenerated ova in this study were 91.4% vs 75.6 %; 75.6% vs 58.9%; 61.5% vs 38.5%; 31.2% vs 5.1%, 8.6% vs 24.4%, 15.7% vs 8% which were significantly different (P<0.01) for treatment CR1aa and KSOM respectively. Based on this study, CR1aa medium is better culture medium than KSOM for efficient in vitro production (IVP) of bovine embryos. Key words: Oocytes, in vitro fertilization, embryo, blastocyst, culture medium

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Abstract

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Home Page

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Contact Name Ahmadi Riyanto

Contact Email medpub@litbang.deptan.go.id

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Location Kota bogor, Jawa barat INDONESIA

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Contact Name
Ahmadi Riyanto

Contact Email
medpub@litbang.deptan.go.id

Phone
-

Journal Mail Official
ahmadi_puslitbangnak@yahoo.com

Editorial Address
-

Location
Kota bogor,

Jawa barat

INDONESIA