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Jurnal Ilmu Ternak Veteriner

JITV
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Published by Indonesian Center for Animal Research and Development

ISSN : 08537380 EISSN : 2252696X DOI : -

Core Subject : Health,

Veterinary

Aims JITV (Jurnal Ilmu ternak dan Veteriner) or Indonesian Journal of Animal and Veterinary Sciences (IJAVS) aims to publish original research results and reviews on farm tropical animals such as cattle, buffaloes, sheep, goats, pigs, horses, poultry, as well as non domesticated Indonesian endemic animals, such as deers, anoa, babirusa, etc. Scope Indonesian Journal of Animal and Veterinary Sciences . The journal will consider primary research papers from any source if they make an original contribution to the experimental or theoretical understanding and application of theories and methodologies of some aspects of animal science and veterinary

Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Hewan dan Zoologi

Articles 1,756 Documents

133 134 135 136 137

Preservation of Bacillus pumilus PU4-2 xylanases by immobilization technique into pollard and cation addition T Haryati; P.A Marbun; T Purwadaria
Jurnal Ilmu Ternak dan Veteriner Vol 15, No 1 (2010): MARCH 2010
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Utilization of by-product from agriculture as alternative source of feedstuff has been widely practiced. However their usage is limited due to high fiber content and low nutrient digestibility. The use of specific hydrolizing enzymes, xylanases are gaining importance because of their wide application in various industrial sectors especially in bioconversion of hemicellulosic material. This experiment was done to evaluate the effect of cation addition and immobilization of enzyme into pollard on stability of B. pumilus xylanase. The enzyme extract was purified by precipitation with 75% ammonium sulphate. Four kinds of cation (Ca2+, Fe3+, Mg2+, Zn2+) were added to the purified enzyme, at concentration of 1m M and stored at 4 and 27˚C. For immobilization process, the optimum enzyme concentration that will be added to pollard has been evaluated by analysis of xylanase activity and their recovery. The specific activity of enzyme after precipitation increased 1.8 times, from 420.3 to 765.2 U/mg protein. All cations act as activator which relative activity become 130.6; 139.0; 103.8 and 163.5% respectively. Concentration of 0.5mM Ca2+ and Fe3+ were most able to keep xylanases activity stable at 4˚C. The optimum composition of enzymes and pollard was 1.5 ml for 5 gram of pollard with recovery of xylanases activity of 82.2%. In immobilized enzyme, the activity of enzyme without cation addition is higher than that with addition of Ca2+ and Fe3+. Activity of enzyme stored at 4˚C is more stable than that at 27˚C. Immobilized enzyme is more stable for storage, which lasted for 7 weeks at 27˚C and 12 weeks at 4˚C compared to liquid enzyme which lasted for only 7 days at 27˚C and 13 days at 4˚C. Key words: Xylanase, Bacillus pumilus PU4-2, Preservation, Imobilization, Cation

Determination of production capacity of circulated primordial germ cells (circulated-PGCs) of KUB chicken using lysis buffer ammonium chloride potassium (ACK) Soni Sopiyana; Iman Supriatna; M. Agus Setiadi; Mohamad Fahrudin
Jurnal Ilmu Ternak dan Veteriner Vol 21, No 1 (2016): MARCH 2016
Publisher : Indonesian Center for Animal Research and Development (ICARD)

In poultry embryos, primordial germ cells (PGCs) are progenitor cells for gametes, which have unique migration pathway. Primordial germ cells arise from epiblast in germinal crescent and circulate through the bloodstream for a short period of time, then leave blood vessel to migrate toward gonads. The aim of this study was to determine the potential production capacity of circulated-PGCs of KUB chicken at different developmental stages of embryo using a rapid and simple method. Seventy five KUB chicken fertile eggs were divided into five groups and incubated at 38.5 0C with a humidity of 60%. Hatching was set to the embryonic development stage of 14-18. The blood was collected through dorsal aorta using micropipette under microscope. The collected blood was placed in a 1.5 ml eppendorf tube which was previously filled with 100 µl phosphate buffered saline without Ca2+ and Mg2+ (PBS-) mixed with fetal bovine serum (FBS) with a ratio of 90%:10%. The PGCs were purified using lysis buffer ammonium chloride potassium method. The results showed that average production of circulated-PGCs per embryo of KUB chicken were significantly affected by stage of embryonic development (P <0.05). The average production of circulated-PGCs at stage 14, 15, 16, 17, and 18 were 37.9; 53.5; 49.8; 38.3; and 33.5 respectively. The number of circulated-PGCs was not different among stages 14, 17 nor 18. The highest number of circulated-PGCs of KUB chicken was obtained at stage 15, so that the isolation and collection of PGCs through the blood circulation was recommended in stage 15.Key Words: KUB Chicken, PGCs, Embryonic Development Stage, Ammonium Chloride Potassium

Isolation and identification of acetogenic bacteria obtained from deer rumen and their potential for methanogenesis inhibitor Amlius Thalib
Jurnal Ilmu Ternak dan Veteriner Vol 13, No 3 (2008): SEPTEMBER 2008
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Methanogenesis can be inhibited by various chemicals through different mechanism reaktion. The use of acetogenic bacteria as H2 sink is assumed to be a promising approach. Isolation and identification of acetogenic bacteria obtained from deer rumen had been conducted. Two types of media used for isolation were hydrogen-carbondioxide utilizing acetogens and carbonmonoxide utilizing acetogens. Identification of species of acetogens isolates was based on descriptions of morphology, Gram type, motility, bioreaction results, and oksygen requirement. The compositions of methane and volatile fatty acids (VFA) were determined on minimal media or added with sheep rumen liquid innoculated with pure isolates. The identification results showed that the isolate cultured on media of hydrogen-carbondioxide utilizing acetogens was Acetoanaerobium noterae and the ones cultured on media of carbonmonoxide utilizing acetogens was Acetobacterium woodii. Inoculumn of A. noterae and A. woodii could decreased the composition of methane resulted from substrate fermented by fresh rumen liquid of sheep (CRDF), that is culture of A. noterae added FPM and defaunator decreased methane production by 28.8% (P<0.01), while culture of A. woodii added microbe growth factors (FPM) and defaunator decreased methane production by 20.6% (P<0.05). Composition of VFA resulted from fibrous substrate fermented by inoculumn of CRDF with and without combined with cultures of A. noterae dan A. woodii were not significantly different except for culture of A. noterae combined with FPM and defaunator additives was higher than CRDF (P<0.05) (ie. 122.9 vs. 97.9 mM for total VFA and 73.43 vs. 68.37% for composition of acetic acid). It is assumed that isolates of A. noterae and A. woodii could be functioned as methanogenesis inhibitor according to the reaction of 2CO2 + 4H2 ==> CH3COOH + 2H2O by which reduction of CO2 with H2 producing CH4 can be inhibited or decreased. Their function as methanogenesis inhibitor would be more significant when they are combined with microbial growth factors and defaunator. Kata Kunci: Asetogenik-bacteria, Acetoanaerobium noterae, Acetobacterium woodii, deer, methane

Effect of glutathione and bovine seminal plasma in lactose extender on viability of swamp buffalo frozen semen Riasari Gail Sianturi; B Purwantara; I Supriatna; Amrozi .; P Situmorang
Jurnal Ilmu Ternak dan Veteriner Vol 17, No 3 (2012): SEPTEMBER 2012
Publisher : Indonesian Center for Animal Research and Development (ICARD)

The aim of the study was to investigate the effect on viability of frozen swamp buffalo semen of glutathione and bovine seminal plasma in lactose extender. Semen from two swamp buffalo bulls was collected twice weekly using an artificial vagina. Pooled, good-quality fresh semen was divided into three parts and centrifuged at 3,000 rpm for 15 minutes in preparation for three treatments-substitution of buffalo seminal plasma with zero, 50 or 100% bovine seminal plasma (BS0, BS50 and BS100, respectively). Each semen aliquot was then divided in two parts, on which was diluted with lactose extender containing 1 mM glutathione (GSH) and the other diluted with lactose extender without GSH (0 mM GSH). Extended semen from all six treatments was cooled to 5oC and then frozen in 0.25 ml straws. Mean motility percentages 0 and 30 minutes post thaw (PTM 0′ and 30′) with GSH were 38.33 and 34.29%, significantly higher (P < 0.05) than treatments without GSH (31.67 and 25.95%). PTM 0′ and 30′ were also higher (P < 0.05) with no substitution of bovine seminal plasma (BS0) than when buffalo seminal plasma was replaced with bovine seminal plasma at either 50 or 100%. Averages were 40.00 vs 34.46 and 30.54% (BS0 vs BS50 and BS100) at thawing and 36.96 vs 28.36 and 25.36% 30 minutes post-thaw. Mean percentages of live sperm (LD), intact plasma membrane (MPU) and intact acrosomal membrane (TAU) at thawing were not significantly different with or without addition of GSH. However,at 30′ post thawing, TAU and MPU were significantly higher in GSH treatments than inthose without GSH: 61.50 vs. 58.19% (MPU) and 59.81 vs. 57.38% (TAU). Mean percentages of LD, TAU and MPU 30′post thawing were higher with no substitution ofbuffalo seminal plasma (BS0) (P < 0.05) than to BS50 and BS100 treatments. In conclusion, the addition of glutathione (GSH) improved the quality of frozen swamp buffalo semen, but the partial substitution of buffalo seminal plasma with bovine seminal plasmaprovided no beneficial effects. Key Words: Swamp buffalo, Semen, Antioxidant, Glutathione, Seminal plasma

Estimation of genetic parameters for body weight of Alabio and Mojosari ducks at starter period L. Hardi Prasetyo; Triana Susanti
Jurnal Ilmu Ternak dan Veteriner Vol 12, No 3 (2007): SEPTEMBER 2007
Publisher : Indonesian Center for Animal Research and Development (ICARD)

A selection program is one of many important tools in livestock breeding in improving the quality of breeding stock. The choice of an effective selection method requires some information on the value of genetic parameters for some economically important traits, such as heritabilities and genetic correlation coefiicients. This experiment used 25 drakes and 100 ducks of each Alabio and Mojosari ducks, mated at random 1 drake to 4 ducks. A number of ducklings were obtained from each mating in each population, and their body weights were observed from hatching to 8 weeks old. Results showed that the heritability estimation for body weight to 8 weeks old were generally low either in Alabio or in Mojosari, ranging between 0.061 to 0.227. The highest heritability estimation was obtained for 6-week body weight 0.151 for Alabio and 0.227 for Mojosari ducks. The estimates of genetic correlation among body weights varied widely but generally high. It is concluded that 6-week body weight can be considered as a selection criterion depending on the selection objective in the local Indonesian ducks. Key Words: Heritability, Genetic Correlation, Ducks

Performance of Garut breed rams fed diets containing various cation-anion difference with or without fish oil supplementation Rahmat Hidayat; T. Toharmat; A. Boediono; I.G. Permana
Jurnal Ilmu Ternak dan Veteriner Vol 16, No 3 (2011): SEPTEMBER 2011
Publisher : Indonesian Center for Animal Research and Development (ICARD)

This study was carried out to evaluate the effect of dietary cation-anion difference (DCAD) and fish oil supplementations on dry matter intake (DMI), dry matter digestibility (DMD) and organic matter digestibility (OMD), weight gain, as well as the acidity of urine in Garut breed rams. The experiment was done based on randomized group design with 6 treatments and 3 groups. The dietary treatments were as follows: R0= basal ration (DCAD +14) without fish oil, R1 = basal ration (DCAD +14) with 3% fish oil, R2 = base ration (DCAD +40) without fish oil, R3 = base ration (DCAD +40) with 3% fish oil, R4 = acid ration (DCAD -40) without fish oil, and R5 = acid ration (DCAD -40) with 3% fish oil. All rations contained 150 ppm of zinc and were offered to 18 of Garut breed rams. The results indicated that DCAD +40 and -40 decreased DMI significantly. The ration with DCAD +40 had the lowest DMI. Fish oil supplementation decreased DMI. No differences were observed for DMD and OMD. DCAD +40 and -40 decreased body weight of rams. However, the body weight of rams was very low. Variation of urine pH followed the DCAD pattern. It was concluded that DCAD +40 and -40, as well as fish oil supplementations decreased DMI, body weight and urine pH followed the DCAD pattern. Keys Words: Cation, Anion, Body Weight, Digestibility, Garut Breed Rams

Vaccination of goats with fresh extract from Sarcoptes scabiei confers partial protective immunity Simson Tarigan
Jurnal Ilmu Ternak dan Veteriner Vol 11, No 2 (2006): JUNE 2006
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Protective immunity has been known to develop in animals infested with Sarcoptes scabiei. However, our previous attempt to induce protective immunity in goats by vaccination with fractions of soluble or insoluble mite proteins had been unsuccessful. Degradation or denaturation of protective antigens occurred during vaccine preparation was suggested as one possible cause of the failure. In this study, mite proteins that used to immunise animals were prepared rapidly in order to prevent protein degradation or denaturation. About 150 mg of freshly isolated mites were rapidly homogenised, centrifuged then separated into supernatant and pellet fractions. Twenty-eight goats were allocated equally into 4 groups. Group-1 goats were vaccinated with the whole mite homogenate supernatant, group 2 with the supernatant, group 3 with the pellet, and group 4 with PBS (unvaccinated control). Vaccination was conducted three times, with three-week intervals between vaccinations, using Quil A as adjuvant, and each vaccination using fresh mite homogenates. One week after the last vaccination, all animals were challenged with approximately 2000 live mites. The severity of lesions, scored from 0 (no lesions) to 5 (>75% infested auricle affected), were determined one day, two days, then every week after challenge. Mite challenge caused the development of skin lesions in all animals. No significant differences between vaccinated and unvaccinated animals were observed in regards to the severity of lesions. However, the mite densities in vaccinated animals were significantly lower (P=0.015) than those in unvaccinated control. This study indicates that the protective antigens of S. scabiei are liable to degradation or denaturation and exist in a very low concentration or have vary low antigenicity. This implies isolation of the protective antigens by the conventional approach, fracionation of the whole mite proteins and testing each fractions in vaccination trials, is seemingly inappropriate for S. scabiei. Key Words: Sarcoptes scabiei, Vaccination, Fresh Homogenate, Partial Immunity

Utilization of complete rumen modifier on sheep fed high fibrous forages. Amlius Thalib; Yeni Widiawati; Budi Haryanto
Jurnal Ilmu Ternak dan Veteriner Vol 15, No 2 (2010): JUNE 2010
Publisher : Indonesian Center for Animal Research and Development (ICARD)

A research to improve livestock productivity and lower enteric methane production on ruminant was conducted by manipulation approach on rumen system using a complete rumen modifier (CRM). An in vivo experiment was carried out using twenty four sheep ( mean weight 18 kg) which were distributed into 3 treatment groups of feed additive: I. control ( without treatment: K); II: K + CRM-LG; III: K + CRM-EL. Diet given consisted of fermented rice straw (ad libitum) + concentrate containing 16 % protein (400 g/head/day), and drinking water was given ad libitum. The experiment was conducted for 14 weeks based on completely randomized design. By the end of the experiment, animals were placed in the metabolism cages for 2 weeks (ie. 1 week for adaptation and 1 week for data collection). Rumen liquid of each treated animal was taken for the measurement of rumen characteristics. Parameter measuremed were: total gas production; gas composition of CO2 and CH4; in vitro DMD; NH3 and VFA contents; pH; bacterial and protozoal counts; consumption/ DMI; in vivo DMD; ADG and FCR. The results showed that productivity of sheep was improved by CRM treatments followed by lowered enteric methane production. The ADG values of CRM treatments (71.4 to 73.5 g) were significantly higher (P < 0.05) than that of control (50 g). The improvement of average daily gain was followed by a better feed conversion (P < 0.05) (ie. 10.6 vs. 12.8). The CRM treatments lowered the percentage of CH4 by 24% compared to Control (P < 0.05). The total and composition of VFA of CRM-treated rumen liquor were significantly different (P<0.05) compared to that of rumen liquor of Control (ie. the total VFA: 85.3 vs 73.5 mM and the percentage of acetic acid: 67.8 vs 60.3%). It is concluded that CRM treatment resulted in positive effects on growth of ruminant fed high fibrous forages such as rice straw and could lower enteric methane production. Key Words: Rumen Modifier, Productivity, Enteric Methane, Sheep, Rice Straw

The detection of infectious bronchitis viral antigen by means of immunohistochemical technique in broiler chicken infected with I-269 IB isolate or injected with H-120 live vaccine Rini Damayanti; Darminto .
Jurnal Ilmu Ternak dan Veteriner Vol 6, No 4 (2001): DECEMBER 2001
Publisher : Indonesian Center for Animal Research and Development (ICARD)

A study was carried out to detect the antigen of infectious bronchitis vius (IBV) in broiler chicken by means of immunohistochemical technique. A total of 150 - fourteen days old broiler chicken were divided into three groups i.e. 50 chicken were infected with an IB isolate of I-269, 50 chicken were injected with H-120 life vaccine, and 50 chicken served as un-treated control. Clinical signs and gross pathological changes were observed. Each of five chicken of each group were necropsied at 1, 2, 3, 4, 7, 10, 14, 21, 28, and 35 day(s) post infection/vaccination. The antigen could be detected at one day through 35 days post vaccination/infection. In the vaccinated group, histopathological lesions and the detected antigen were minimal. In contrast, the infected chicken showed varied histolopathological lesion in accordance with the numerous antigens. The antigen were observed in the lymphocytes/macrophages in the trachea, lungs and kidney, and in the epithelium of trachea, alveoli, broncheolus and tubular sitoplasm of the kidney of both vaccinated and infected groups. In the infected group, antigen was also detected in the lymphocytes and macrophages of the affected organs. Key words: Infectious bronchitis, broiler chicken, I-269 IB isolate, immunohistochemistry

Purification and production of monospecific antibody to the hemagglutinin from Subtype H5N1 influenza virus Simson Tarigan; R Indriani; D Hewajuli
Jurnal Ilmu Ternak dan Veteriner Vol 15, No 4 (2010): DECEMBER 2010
Publisher : Indonesian Center for Animal Research and Development (ICARD)

The purpose of this study was to purify the hemagglutinin from H5N1 virus and to generate monospecific antibody appropriate for production of sensitive and specific immunoassay for H5N1 avian influenza. For this purpose, a local isolate H5N1 virus (A/Ck/West Java/Hamd/2006) was propagated in chicken embryos. The viral pellet was dissolved in a Triton-X-100 solution, undissolved viral particles were pelleted by ultracentrifuge, and the supernatant containing viral surface glycoproteins (Hemagglutinin and neuraminidase) was collected. The neuraminidase in the supernatant was absorbed by passing the supernatant through an Oxamic-acid-superose column. After dialyzing extensively, the filtrate was further fractionated with an anion exchange chromatography (Q-sepharose) column. Proteins adsorbed by the column were eluted stepwisely with 0.10, 0.25, 0.25 and 0.75 M NaCl in 20 mM Tris, ph 8. Hemagglutinin (H5) was found to be eluted from the column with the 0.5 M NaCl elution buffer. The purified H5 was free from other viral proteins based on immunoassays using commercial antibodies to H5N1 nucleoprotein and neuraminidase. When used as ELISA’s coating antigen, the purified H5 proved to be sensitive and specific for hemagglutinin H5. Cross reactions with other type-A-influenza virus, H6, H7 dan H9, were negligibly low. For the production of monospecific antiserum, the purified H5 was separated with SDS-PAGE, the band containing the H5 monomer was cut out , homogenised and injected into rabbits. The antiserum was capable of detecting the presence of inactivated H5N1 virus in a very dilute suspension, with a detection limit of 0.04 heagglutination (HA) unit. The purified hemagglutinin and the serum raised against it should be useful for developing specific, sensitive and affordable immunoassay for H5N1 avian influenza. Key Words: H5N1, Hemagglutinin, Triton, Oxamic-acid Sepharose, Q sepharose, ELISA

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Ahmadi Riyanto

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medpub@litbang.deptan.go.id

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Journal Mail Official
ahmadi_puslitbangnak@yahoo.com

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Location
Kota bogor,

Jawa barat

INDONESIA