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Jurnal Ilmu Ternak Veteriner

JITV
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Published by Indonesian Center for Animal Research and Development

ISSN : 08537380 EISSN : 2252696X DOI : -

Core Subject : Health,

Veterinary

Aims JITV (Jurnal Ilmu ternak dan Veteriner) or Indonesian Journal of Animal and Veterinary Sciences (IJAVS) aims to publish original research results and reviews on farm tropical animals such as cattle, buffaloes, sheep, goats, pigs, horses, poultry, as well as non domesticated Indonesian endemic animals, such as deers, anoa, babirusa, etc. Scope Indonesian Journal of Animal and Veterinary Sciences . The journal will consider primary research papers from any source if they make an original contribution to the experimental or theoretical understanding and application of theories and methodologies of some aspects of animal science and veterinary

Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Hewan dan Zoologi

Articles 1,756 Documents

134 135 136 137 138

Microencapsulation of bovine spermatozoa: Cryopreservation of microencapsulation sperm using glycerol Diana Andrianita Kusumaningrum; Bambang Purwantara; Tuti L. Yusuf; Polmer Situmorang
Jurnal Ilmu Ternak dan Veteriner Vol 20, No 4 (2015): DECEMBER 2015
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Cryopreservation of spermatozoa has been used to preserve spermatozoa in very low temperatures. Glycerol is intracelullar cryoprotectant usually used in Tris citrate containing 20% egg yolk (TCEY), while TCEY-1.5% alginate was used as encapsulation media. The effect of alginate in microencapsulation process and glycerol concentration on viability spermatozoa (motility (%M), live sperm (%L) and intact apical ridge (%IAR) were studied in two steps. In the first step, the effect of alginate and microencapsulation process was studied. Three treatments of this step were: 1) TCEY add with semen (as control), 2) TCEY-Alginate add with semen, 3) TCEY-Alginate add with semen and microencapsulated, followed by conventional sperm cryopreservation using TCEY 7% glicerol. The second steps were done to evaluate the effect of two glycerol concentrations (5 and 7%) and two duration of equilibration time (3 and 4 hours). Result of these experiments showed, that microencapsulation was significantly alter the percentage of post-thawing motility (%M) and intact apical ridge (%IAR). The motility of microencapsulated sperm vs control was 44.7 and 50.83% respectively, whereas IAR 79.33% and 83.50% on microencapsulated sperm and control. There was evidence that alginate act as extracelullar cryoprotectant by protecting sperm during freezing. The mean of decreasing percentage of %M and %IAR in TCEY-Alginate were lower (15.97 and 6.44%) than control (23.80 and 7.37%). The effect of glycerol concentration and equilibration time on the viability of sperm was not significant different. There was no significant interaction of glicerol and equilibration time on the viability of spermatozoa. In conclution, microencapsulation processes altered the viability of sperm, alginate had an important roles as extracelullar cryoprotectant. Moreover the cryopreservation of microencapsulated sperm might be done using 5 or 7% glycerol in 3-4 hours duration of equilibration time. Key Words: Sperm, Alginate, Microencapsulation, Glycerol, Equilibration Time

Analysis of genetic relationship among Indonesian native chicken breeds based on 335 D-loop sequences Sri Sulandari; M. Syamsul Arifin Zein; Tike Sartika
Jurnal Ilmu Ternak dan Veteriner Vol 13, No 4 (2008): DECEMBER 2008
Publisher : Indonesian Center for Animal Research and Development (ICARD)

The Mitochondrial DNA (mtDNA) D-loop segment was PCR amplified and subsequently sequenced for a total of 335 individuals from Indonesian native chicken. The individuals were drawn from sixteen populations of native chicken and three individuals of green jungle fowls (Gallus varius). Indonesian native chicken populations were: Pelung Sembawa, PL (n = 18), Pelung Cianjur, PLC (n = 29) and Arab Silver, ARS (n=30), Cemani, CM (n = 32), Gaok, GA (n = 7), Kedu Hitam, KDH (n = 11), Wareng, T & TW (n = 10), Cemani, CMP (n = 2), Kedu, KD (n=26), Kedu Putih, KDP (n = 15), Sentul Jatiwangi, STJ (n = 27), Ayam Kate, KT (n = 29), Ayam Sentul, STC (n = 15), Arab Golden, ARG (n = 26), Ayam Merawang, MR (n = 28), Kedu Putih Jatiwangi, KDPJ (n=6) and Kapas, KPS (n = 21). Green jungle fowls were: two individuals from Flores island (FL5 and FL57) and one individual (BD42) from Sumbawa island. The sequences of the first 530 nucleotides were used for analysis. Eighty two haplotypes were identified from 78 polymorphic sites for the 335 individuals. Seventy nine haplotypes were identified in native chicken from 57 polymorphic sites while three were of jungle fowls. Phylogenetic analysis indicates that Indonesian native chicken can be grouped into five clades (Clade I, II, IIIc, IIId and IV) of the previously identified seven clades (Clade I, II, IIIa, IIIb, IIIc, IIId and IV) in Asian domestic chicken. Haplotypes CM10 and CM32 fall to a different category while STC12 is also on its own. Interestingly STC12 clusters together with Gallus gallus gallus (GenBank accession No. SULANDARI et al. Analysis of genetic relationship among Indonesian native chicken breeds based on 335 D-loop sequences 296 AB007720). When CM10 (same as CM14), CM32 and STC12 were removed, 77 haplotypes of domestic chicken were identified from 53 polymorphic sites. All the green jungle fowls are clustered to one clade of their own. The clades of domestic chicken are: Clade I which has three haplotypes, Clade II has 52 haplotypes, Clade IIIc has one haplotype (represented by ARS30), Clade IIId has nine haplotypes while Clade IV has eleven haplotypes. The phylogenetic relationship between chicken populations has no link to the geographic locations. Analysis of molecular variance showed that the genetic variation within populations was 67.42% while 32.58% accounted for the genetic differentiation between populations. Key Words: Native Chiken, Green Jungle Fowls, D-Loop DNA Mitochondria, HV-1, Clade, Haplotype, Phylogenetic, Genetic Variation

Pre-weaning growth performance of Etawah Crossbred goats fed milk replacer Supriyati .
Jurnal Ilmu Ternak dan Veteriner Vol 17, No 2 (2012): JUNE 2012
Publisher : Indonesian Center for Animal Research and Development (ICARD)

In this experiment the effect of feeding milk replacer and dairy milk on the growth performance of pre-weaning of Etawah Crossbred goats was studied. Thirty eight heads of pre-weaning of Etawah Crossbred kids were divided into 2 groups, Group A receiving dairy milk and Group B receiving formulated milk replacer. All kids were fed with colostrum at the first 3 days. The milk replacer was formulated from skim milk, cassava flour, soy flour, corn flour, vitamin, mineral, salt and amino acids (lysine and methionine). The milk replacer was diluted ten times with warm water, then probiotic and sugar were added. The pre-weaning kids fed 300-600 ml milk twice a day, in the morning and in the afternoon. The liveweight of pre-weaning kids were measured every 2 weeks. The experiment was carried out for 12 weeks. The parameter measured were nutrient intakes, ADG and mortality rate. Data was analysed by T test. The proximate analysis results of milk replacer were DM 93.50%, CP 22.20%, Fat 4.62%, GE 3869 kcal/kg, CF 1.31%, ash 4.22%, Ca 0.60% and P 0.46%. The DM and CP intakes were 111.98 and 28.93 g/d; 97.31 and 23.10 g/d, with ME 659 and 379 kcal/kg for group A and B, respectively. The ADG of pre-weaning kids for Group A and B were significantly different (P < 0.05) which were 96.03 ± 11.83 and 83.62 ± 16,34 g/d; mortality rates were 0% and 10% for group A and B, respectively. It is concluded that the rate of the pre-weaning kids of Crossbred Etawah goat fed formulated milk replacer grew slower than kids receiving dairy milk. Key Words: Milk Replacer, Pre-Weaning, Growth, Crossbred Etawah Goats

The macroscopic and microscopic patology changes on broiler infected with local reovirus isolate Sutiastuti Wahyuwardani; Hernomoadi Huminto; Lies Parede
Jurnal Ilmu Ternak dan Veteriner Vol 10, No 1 (2005): MARCH 2005
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Local reovirus isolate is the virus which could be isolated from runting and stunting syndrome. The ability of local reovirus isolate to induce runting and stunting syndrome in broilers chicken was investigated. The day old chicks (doc) were infected with local reovirus isolate assessed clinically and pathologically at 1, 2 and 3 weeks post inoculation. A total of 40 DOC were divided into two groups. The first group (20 doc) was orally infected with ± 2 x 103 local reovirus isolate particle and the other used as negative control group. The results showed that the isolate caused wet droppings, stunting, enteritis, pancreatitis, malabsorbtion, bursal atrophy and spleenic hypertrophy, which similar to runting and stunting syndrome (RSS) disease in chicken. The body weight was reduced to 14.7% on the inoculated group at 4 weeks post inoculation. Key Words: Broiler, Local Reovirus Isolate, Runting and Stunting, Malabsorption

Addition of Ca-soap of Sardinella longiceps oil into the ration: 2. Its effect on physical and chemical characteristics of lamb A Sudarman; M Muttakin; H Nuraini
Jurnal Ilmu Ternak dan Veteriner Vol 13, No 2 (2008): JUNE 2008
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Lamb contains high saturated fatty acids and cholesterol. Consuming such meat tends to increase atherosclerosis incident. Fat content and composition of meat might be manipulated by giving lemuru fish oil which is rich in HDL and unsaturated fatty acids into sheep diet. Giving lemuru fish oil in the protected form, such as complex calcium-soap is believed to be more beneficial. Twelve sheep were distributed into four groups to receive four experimental diets in studying the influence of calcium soap on meat quality. The sheep were allocated based on Randomized Block Design and at the end of rearing period they were slaughtered. The experimental diets were: R0 = control diet, R1 = R0 + 1.5% calcium soap, R2 = R0 + 3% calcium soap and R3 = R0 + 4.5% calcium soap. Data were analyzed using analysis of variance and any significant differences were further tested using contrast orthogonal. The results showed that the treatments significantly (P<0.01) reduced fat content, both of low density lipoprotein cholesterol (LDL), and high density lipoprotein cholesterol (HDL) of sheep meat. There were no differences in tendernes, cooking loss, water holding capacity (WHC) and pH of meat. It is concluded that the best level of Ca-soap fish oil addition to yield healthy meat is 3%. Key Words: Calcium-Soap, Lamb Meat, Fat, HDL-Cholesterol, LDL-Cholesterol

Biolarvacidal activity of methanol extract of Kipahit leaves (Tithonia diversifolia) against larvae of Chrysomya bezziana fly Wardhana AH; Diana N
Jurnal Ilmu Ternak dan Veteriner Vol 19, No 1 (2014): MARCH 2014
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Myiasis or “belatungan” is the infestation of live human and vertebrate animal tissue with dipterous larvae, Chrysomya bezziana. In general, synthetic insecticides were applied to control the disease. However, it causes negative impact on animal product, so that it is required to find an alternative treatment using herbal medicine. The aim of this study was to access activity of methanol extract of Kipahit leaves (Tithonia diversifolia) against various stages of C. bezziana larvae (L1, L2 and L3). Five treatments were applied with five replications, i.e. control/water (P0), 0,5%, 1%, 2% of the extract for PI, PII and PIII, respectively. Another treatment was 0,05% Asuntol for positive control (PIV). Each treatment was added with1% DMSO and twenty larvae were examined for each replication. Bioassay of L1 and L2 was addressed to investigate effect of intestinal toxicity by mixing the extract with Meat-Blood Mixture (MBM) and Larval Rearing Media (LRM) for L1 and L2, respectively. Bioassay of L3 was to investigate effect of contact toxicity through soaking the larvae into the extract solution for 10 seconds followed by incubating in vermiculite at 36oC. All living larvae after being treated by various concentration of the extract were reared to pupae and allowed to emerge as imago. Number of living larvae and pupae, pupae weight and number of imago were observed. All data were analysed using ANOVA followed by Dunnett test at 95% confidential limit. For L2, larval mortality were counted and probit analysed using POLO-PC software, therefore the lethal concentration (LC50 and LC95) and lethal time (LT50 and LT95) were defined. Results demonstrated that 1% of the extract was the most effective concentration which was able to kill the larvae and decrease the pupae weight. It also caused to fail pupation and imago emergence. The further study might be pursued to investigate in vivo assay of the extract in infested livestock. Key Words: Tithonia diversifolia, Myiasis, Chrysomya bezziana, Biolarvasidal

Pesticide residues in brain tissues of dairy cattle in Lembang Indraningsih Indraningsih; Yulvian Sani
Jurnal Ilmu Ternak dan Veteriner Vol 11, No 1 (2006): MARCH 2006
Publisher : Indonesian Center for Animal Research and Development (ICARD)

The use of pesticides to control plant diseases may cause residual formation in crops, its byproduct and environmental. Furthermore, the use of agriculture byproduct as animal feed may cause poisoning or residual formation in animal products. The purpose of this study is to investigate of pesticide residues in brain tissues of dairy cattle in relation to animal feed as a contamination source. Samples consisted of animal feeds (19 samples of fodder and 6 samples of feed), 31 samples of sera and 25 samples of brain tissues of dairy cattle collected from Lembang, West Java. Feeds and fodders were collected from dairy farms located in Lembang. Sera were directly collected from 31 heads of Frisien Holstein (FH) cattle from the same location, while brain tissues of FH cattle were collected from a local animal slaughtering house. Pesticide residues were analysed using gas chromatography (GC). Both residues of organochlorines and organophosphates were detected from brain tissues with average residue concentration OP was 22.7 ppb and OC was 5.1 ppb and a total residue was 27.8 ppb. The pesticide residues in brain tissues are new information that should be taken into consideration since the Indonesian consumed this tissues as an oval. Although pesticides residue concentration was low, pathological changes were noted microscopically from the brain tissues including extracellular vacuolisation, focal necrosis, haemorrhages, dilatation of basement membrane without cellular infiltration. Both pesticide residues were also detected in sera, where OP (9.0 ppb) was higher than OC (4.9 ppb). These pesticides were also detected in animal feeds consisting fodders and feeds. Residues of OP (12.0 ppb) were higher than OC (1.8 ppb) in feeds, but residues of OP (16.8 ppb) were lower than OC (18.7 ppb) in fodders. Although, pesticide residues in sera and brain tissues were below the maximum residue limits (MRL) of fat, the presence of pesticides in brain tissues should be taken into consideration as their effects in brain lesions. There was a correlation between contaminant found in animal feeds and pesticide residues in sera and brain tissues of dairy cattle.Key Words: Pesticides, Residues, Brain, Dairy Cattle

Performance, carcass precentages and heterosis values, Alabio and Cihateup line and crossbreeding on eight week old P.R. Matitaputty; R.R. Noor; P.S. Hardjosworo; C.H. Wijaya
Jurnal Ilmu Ternak dan Veteriner Vol 16, No 2 (2011): JUNE 2011
Publisher : Indonesian Center for Animal Research and Development (ICARD)

One effort for improving the productivity of Indonesian local ducks in particular as a meat types is by crossing among the local ducks. Crossbreeding is a fast breeding program for improving some commercial traits. Crossing between two lines or different breed are often used in production systems to utilized the advantage of heterosis (hybrid vigor). Crossbreeding is expected to produce new synthetic strains of duck that perform better than their parents. This study aims to utilize the heterosis and determine best cross, and to study the performance changes due to crossbreeding. The statistical design used in this study was a randomized complete design (RCD) consisting of 4 treatments with 6 replications, each replication consisting of five ducks. The treatments used were the crossing of the pure Cihateup ♂ x Cihateup ♀ (CC), line Alabio ♂ x Alabio ♀ (AA), Alabio ♂ x Cihateup ♀ (AC) cross as well as Cihateup ♂ x Alabio ♀ (CA) cross. This study using male ducklings first hybrid (F1). The results show that the CA is better than AC in the final live weight (7.05%), weight gain (7.32%), carcass weight (9.24%) and carcass yield (2.55%). Fleshy carcass cuts such as breast meat of AA (6.13%) is better when compared to the others, but the highest percentage of meat percentage of the thigh is the AC (10.13%). Duck crossesing CA possess superior properties more and economic value compared with the duck AC. Key Words: Alabio Duck, Cihateup Duck, Crossing, Heterosis, Performance

Using follicle stimulating hormone (FSH) for superovulation in buffalo Polmer Situmorang
Jurnal Ilmu Ternak dan Veteriner Vol 11, No 4 (2006): DECEMBER 2006
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Studies of superovulation have been carried out in buffaloes and the progress has been achieved but the number of embryo collected was still small. Two studies were conducted to evaluate the use Follicle Stimulating Hormone (FSH) for superovulation in buffalo. First study, fifteen buffaloes were randomly divided into 3 groups of five buffaloes of each group and superovulated using three type of FSH (FSH-P; FSC-China and Folltropin). Hormones were given twice a day for 4 days in decreasing doses methods. First injection initiated on day 10 of estrus cycle, followed by prostaglandin on day 12 than artificially inseminated (AI) two days later. Recovery of embryo was conducted un-surgically method by flushing uterus horn on day 6 after AI. Second study, 10 buffaloes were randomly divided into 2 groups of each five buffaloes First group was superovulated with FSH followed methodology in the first study (control group) and second group were given a prime FSH on day 1 of estrus cycle before superovulation (treatment group). After 3 months the superovulation was repeated in the reverse condition where the control become treatment and the treatment become a control group. The type of FSH did not significantly affect the superovulatory response. The mean total corpus luteum (TCL), total number of embryo (TNE) and total number of viable embryo (TVE) were 6.8, 3.3 and 2.2, 6.2, 3.0 and 2.1 and 7.2, 3.6 and 2.4 for FSH-P, FSH China and Folltropin respectively. A single injection of FSH significantly increased (P<0.05) the number of embryo collected. The mean TCL, TNE and TVE were 6.9, 2.8 and 2.1 and 8.2, 3.3 and 2.5 for control and treatments respectively. The peak progesterone level was significantly higher (P<0.05) in treatment group (6.8 ng/ml) than those in control (5.6 ng/ml). Repeated the superovulation after 3 months did not significantly affect the number of embryo collected. In conclusion, superovulation in buffaloes can be performed using a types of FSH and prime injection of FSH on day 1 of estrus cycle increase the number of embryo recovered. The superovulation can be conducted after 3 months. Key Words: FSH, Superovulation, Embryo

Morphometric performances of Katingan cattle in Central Kalimantan Bambang Ngadji Utomo; R.R Noor; C Sumantri; I Supriatna; E.D Gunardi
Jurnal Ilmu Ternak dan Veteriner Vol 15, No 3 (2010): SEPTEMBER 2010
Publisher : Indonesian Center for Animal Research and Development (ICARD)

The information of Katingan cattle as an indigenous cattle in Central Kalimantan is very limited, especially phenotypic characterization. A number of 72 heads, consist of 57 heads female and 15 heads male of Katingan cattle in adults category were used in this study. The samples were obtained from three sub districts in district of Katingan, those were Tewah Sanggalang Garing (Pendahara Village), Pulau Malan (Buntut Bali Village) and Katingan Tengah (Tumbang Lahang Village). The location were used as the variable of groups (subpopulation). This study was conducted to describe morphometric performance, which was analysed by discriminant and canonical to estimate the phylogenic relationship and to determine the discriminant variable among the group (subpopulation). The SAS package program version 6.0 and MEGA 2 program was used to analyze data. The observed parameters were body length, wither height, chest width, chest depth, chest circumference, hip heigth, hip width, head height, head length and head width. The chest depth and hip height of female cattle from group of Buntut Bali were lower (P < 0.05) than that of Pendahara and Tumbang Lahang groups. The parameter of: chest width, hip width and body length of Buntut Bali groups were higher (P < 0.05) than that of Pendahara and Tumbang Lahang groups. The average body weight of male Katingan cattle from Buntut Bali was higher (P < 0.05) than that of Pendahara and Tumbang Lahang groups but there were not different (P > 0.05) on bodyweight of female Katingan cattle among location. The highest similarity value was Katingan cattle from group of Tumbang Lahang (92,86%) followed by group of Pendahara (40.91%) and group of Buntut Bali (38.89%), repectivelly. Katingan cattle from group of Pendahara and Tumbang Lahang had closest genetic distance (1.406), while the longest genetic distance (2.046) was between group of Buntut Bali and group of Tumbang Lahang. Genetic distance between group of Buntut Bali and Pendahara was 1.929. Katingan cattle from Pendahara and Tumbang Lahang within one cluster. Body length in canonical structure 2 can be used as discriminant variable. Key Words: Katingan Cattle, Morphometric, Genetic Distance, Discriminant Analysis, Canonical

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Ahmadi Riyanto

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medpub@litbang.deptan.go.id

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Journal Mail Official
ahmadi_puslitbangnak@yahoo.com

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Kota bogor,

Jawa barat

INDONESIA