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Jurnal Ilmu Ternak Veteriner

JITV
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Published by Indonesian Center for Animal Research and Development

ISSN : 08537380 EISSN : 2252696X DOI : -

Core Subject : Health,

Veterinary

Aims JITV (Jurnal Ilmu ternak dan Veteriner) or Indonesian Journal of Animal and Veterinary Sciences (IJAVS) aims to publish original research results and reviews on farm tropical animals such as cattle, buffaloes, sheep, goats, pigs, horses, poultry, as well as non domesticated Indonesian endemic animals, such as deers, anoa, babirusa, etc. Scope Indonesian Journal of Animal and Veterinary Sciences . The journal will consider primary research papers from any source if they make an original contribution to the experimental or theoretical understanding and application of theories and methodologies of some aspects of animal science and veterinary

Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Hewan dan Zoologi

Articles 1,756 Documents

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Effect of Ascaridia galli infection on histopathologic description, size of small intestines villi surface and body weight change in starters Zalizar, L; F, Satrija; R, Tiuria; Astuti, D.A
Indonesian Journal of Animal and Veterinary Sciences Vol 11, No 3 (2006)
Publisher : Indonesian Animal Sciences Society

Nematode Ascaridia galli is an important parasitic disease in poultry and is responsible for considerable economic losses in retarded growth and lowered egg production. The effects of A. galli infection based on histopathologic description, size of small intestines villi surface and body weight change in starters was investigated. One hundred and thirty five day old chicks (DOC) were divided into three groups for three levels of infection dose rate (0,800 and 8000 infective eggs) with 3 replications of 45 DOC each. Infections were carried out every week respectively from week 2th until week 5th. Results showed that the infection of A. galli caused degeneration and necroses in villi ephitelial cells and crypts of small intestine and infiltration of leucocytes. In the heavy infection group some epithelial cells were replaced by fibrocytes. A. Â galli infection decreased daily body weight gain of starter lower (5.5% in light and 13.4% in heavy dosage infection) compared to that of the non infected group. After six weeks of heavy infection the size of small intestine villi surface was decreasing to 20.0%, while the daily body weight gain was decreasing to 12.3% compared to that of the non infection group. Key words: Ascaridia galli, Starter, Productivity

Diagnosis of Fasciola gigantica infection in cattle using capture-ELISA assay for detecting antigen in faeces Estuningsih, Sarwitri Endah
Indonesian Journal of Animal and Veterinary Sciences Vol 11, No 3 (2006)
Publisher : Indonesian Animal Sciences Society

Capture-ELISA assay is a diagnosis for antigen detection in the serum or faeces using polyclonal or monoclonal antibodies. The purpose of this study was to determine the sensitivity and specificity of capture-ELISA assay using polyclonal antibody for diagnosing Fasciola gigantica infection in cattle by detecting antigen in the faeces. In this study, faecal samples and livers were collected from 141 cattle slaughtered in the abattoir in Jakarta. From each animal, liver was processed for liver flukes count and the corresponding faecal sample was analysed for coproantigen. The result of capture-ELISA assay for antigen detection showed that from 85 cattle infected with Fasciola gigantica, 83 had OD > 0.52 (range from 0.52-1.39) and 2 cattle had OD < 0.52 (range from 0.17-0.51). The sensitivity and specificity of the assay were 97.6% and 92.8% respectively. The assay also able to detect 50 ng/ml of antigen in faecal supernatant. It suggests that this assay will have the advantage over the other methods on its ability to detect the active infection. Collection of faeces, rather than serum, will allow a more cost-effective and adaptable method. Key Words: Fasciola gigantica, Diagnosis, Capture-ELISA, Antigen

Sequence variability of the Î² Tubulin isotype-1 genes in benzimidazole resistant strains of Haemonchus contortus, a nematode parasite of sheep Haryuningtyas, Dyah; Artama, wayan T; Asmara, Widya
Indonesian Journal of Animal and Veterinary Sciences Vol 11, No 3 (2006)
Publisher : Indonesian Animal Sciences Society

Resistance to benzimidazole at a population of gastrointestinal nematode, especially Haemonchus contortus is a widespread problem. Therefore, anthelmintic will not effective anymore. Benzimidazole resistance in nematode has been shown to be heritable and defined as an increase in frequency of individual worms able to drug tolerate. In Indonesia resistance to anthelmintic albendazole has been reported on sheep in district of Bogor, Kuningan, Kendal and province of Yogyakarta. Molecular mechanism of resistance to benzimidazole on nematode and fungi related to single gene which code Ã tubulin. The aim of this research is to determine a genetics variability of isotype-1 Ã tubulin genes from Indonesian strain of H. contortus which resistant to benzimidazole. Sequencing were carried out at a 520 bp fragment of the isotipe-1 Ã tubulin gene of six H. contortus resistant strain from four sheep from SPTD Trijaya farm, district of Kuningan, West Java; UPTD Pelayanan Kesehatan Hewan farm, district of Bantul, Yogyakarta respectively. One H. contortus susceptible strains as a control come from farmer from Cicurug, district of Sukabumi, West Java. Results indicated that there are genetics variability ofÂ fragment isotype-1 Ã tubulin genes from H. contortus from UPTD Pelayanan Kesehatan Hewan farm, Bantul, Yogyakarta (H1, H2, and C2). There are no genetics variability in the fragment gene of H. contortus resistant strain from SPTD Trijaya farm, Kuningan, West Java (Kn1, Kn2 and K1). Genetic variability occur between 2 isolate from different location. Key Words: Haemonchus contortus, Benzimidazole, Isotype 1 Î²-tubulin Gene, Sequencing

Development of enzyme-linked immunosorbent assay for detecting Ornithobacterium rhinotracheale (ORT) infection in chicken Priadi, Adin; Natalia, Lily
Indonesian Journal of Animal and Veterinary Sciences Vol 11, No 3 (2006)
Publisher : Indonesian Animal Sciences Society

Ornithobacterium rhinotracheale (ORT) has been recognized in chicken in Indonesia and incriminated as a possible additional causative agent in respiratory disease complex. An enzyme-linked immunosorbent assay (ELISA) has been developed for the seroepidemiological study of ORT infection in chickens. Ten weeks old chickens are injected with 0.5 ml of killed O.Â rhinotracheale emulsified in Freunds complete adjuvant at a concentration of 109 CFU/ml. Hyperimmune sera and non-reactive control sera were used to standardized the ELISA for ORT infection. Optimum condition for the ORT ELISA was antigen dilution 1/800, serum dilution 1/100 and 1/4000 conjugate dilution. Optical density cut-off point was determined by using 31 serum samples from 2 broiler farms. Cut-off for negative serum was 0.27 (mean + 3 standard deviation). With these optima, 187 chicken sera from broiler, layer and broiler breeder farms were collected and screened. Seroconvertions were detected from broiler and layer farms in Magelang district, Central Java (Bojong I, Paremono, Bojong II, Keblukan) and a broiler breeder farm in West Java. The seraconvertion were 0, 10, 94, 88 and 100 percents respectively. These figures show that the prevalence of O. rhinotracheale infection in chicken in layer and breeder farms were very high. Key Words: Ornithobacterium rhinotracheale (ORT), ELISA, Chicken

Supplementation of vitamin C as anti heat-stress agen of broilers Kusnadi, Engkus
Indonesian Journal of Animal and Veterinary Sciences Vol 11, No 4 (2006)
Publisher : Indonesian Animal Sciences Society

High environmental temperatures may cause heat stress in poultry. This may increase water consumption, decrease feed consumption and in turn, decrease production level.Â This experiment was conducted to study the supplementation of vitamin C as anti heat-stress agent of broilers. In this research, were used 72 broilers 21 days old. The treatment are two kinds. The first, two poultry house temperatures (32 and 240C as warm temperature and cool temperature respectively) and second, four levels suplementation of vitamin C (0, 250, 500 and 750 ppm of ration as C0, C250, C500 and C750 respectively). Variables measured are feed consumption, body weigh gain, content of abdominal lipid and feed convertion. The data collected were analized with a split plot design in completelly random design and continued with the contrast-orthogonal test when significantly different The result indicated that vitamin C significantly (P<0.05) increased feed consumption and body weigh gain. The warm temperature significantly (P<0.05) decreased feed consumption and body weigh gain. Feed convertion of C250, C500 and C750 in warm temperature were significantly (P<0.05) lower than C0. The supplementation of 250 ppm vitamin C (C250) was most efective as anti heat-stress agent in broilers kept at warm temperature. Key Words: Vitamin C, Broiler, Heat-Stress

Utilization of Katuk (Sauropus androgynus L Merr) meal and extract as ration substitution to produce low chollesterol Japanese Quail product Subekti, Sri; Piliang, Wiranda G; Manalu, Wasmen; Murdiati, Tri Budhi
Indonesian Journal of Animal and Veterinary Sciences Vol 11, No 4 (2006)
Publisher : Indonesian Animal Sciences Society

Katuk (Sauropus androgynus L Merr) is known to contain carotenoids, vitamin E, vitamin C, protein and phytosterol compound. This study was aimed to determine the ability of phytosterols in Sauropus androgynus (SA) leaf to obtain poultry product that has low cholesterol content. One hundred fifty female quails were raised from 2-27 weeks old, divided into three treatment diets, five replicates with 10 quails in each replicate. The treatment diets were: 1) Control group: diet without katuk leaf meal; 2) Diet with 9% SA ethanol 70% extract (SAE); 3) Diet containing 9% SA meal (SAM). Kolesterols were determined with CHOD-PAP-Method by Human, there is enzymatic Colorimetric Test for Cholesterol with Lipid Clearing Factor. The result showed that total cholesterol in the egg yolk, carcass and liver of SAE and SAM treated quails were lower (P<0.05) than that of the control-treated quails, except the cholesterol content in the serum. These findings indicated that the cholesterol concentration of female quails was decreased due to phytosterol content in katuk leaf. Key Words: Phytosterol, Katuk Leaf, Kolesterol, Quails

The change in cholesterol content of long chain fatty acid egg during processing and its influence to the Rattus norvegicus L. blood cholesterol content Hardini, Dini; Yuwanta, Tri; ., Zuprizal; ., Supadmo
Indonesian Journal of Animal and Veterinary Sciences Vol 11, No 4 (2006)
Publisher : Indonesian Animal Sciences Society

Egg containing long chain unsaturated fatty acids is a functional food, because it is highly nutritious and could prevent diseases, (omega 3 and 6) such as coronary heart attack. The research was aimed to measure the change of egg cholesterol contentÂ during proceesing: frying, oiless frying and boiling and their influence to the blood plasma cholesterol of normal and hypercholesterolemia rat. Seven treatments of egg yolk were frying at 170Â°C for 3 min (welldone = GM), and 1min (half medium fried = GSM) using deep fryer , oilless frying at 70Â°C for10 min (fried = TM), and 6 min (half fried = TSM) using Teflon pan, and boiling at 100Â°C for 10â (boiled = RM) dan 4 min (half boiled = RSM) using pan provided with thermoregulator and a fresh omega egg as a control. The Completely randomized design was apllied for 4 weeks research period. The data from different treatments were analyzed by Orthogonal Contrast. Fifty 2 months old male rats Rattus norvegicus L. separated in 2 groups; normal and hypercholesterolemia (blood cholesterol > 200 mg dl-1). The rats were placed in individual cage, fed 15 g h-1 day-1 and water drinking ad libitum. The ration was composed of 90% basal commercial feed BR II and 10% egg yolk was given to each animal at 20% of live weight. Factorial 2 x 7 of completely randomized design was applied. The data were analyzed by ANOVA and Duncanâs Multiple Range Test. Processsing method of egg affected to cholesterol content of egg, The lowest and the highest cholesterol contents were observed in TSM (0.30 g/100g) and GM (0.37 g/100g), respectively. Biological test using Rattus norvegicus L rat showed that either fresh and processed long chain fatty acid egg decreased plasma cholesterol.Â The highest and the lowest decreases of cholesterol content were found in the group consumed RSM (8.64%) and GM (1.77%) for normal rat; and control (46.3%) followed by RSM (44.53%) and GM (24.86%), respectively.Â To maintain normal cholesterol and decrease cholesterol content of cholesterolemian, the omega egg should be consumed in fresh or half boiled egg. Key Words: Cholesterol, Omega-3, Omega-6, Rattus norvegicus L.

Comparison of fermentation kinetics (in vitro) of grass and shrub legume leaves: The pattern of gas production, organic matter degradation, pH and NH3 production Widiawati, Y; Thalib, A
Indonesian Journal of Animal and Veterinary Sciences Vol 11, No 4 (2006)
Publisher : Indonesian Animal Sciences Society

Rumen microbes convert carbohydrate in a feed to useful product such as VFA, microbial protein and B-vitamin; and some waste products such as CH4 and CO2. The amount and pattern of each product depend on the type of feed Sonsumed by the animal. High fiber diet produce high gas and VFA, while high protein diet produce high NH3. Two types of feeds, grass and legume, have different cell structure and content, thus might have different pattern of rumen fermentation and product. In vitro method developed by Theodorou and Brooks was used to determine the pattern of rumen fermentation and product of two types of feeds, namely grass and legume. Result shows that legume has higher amount of OM degraded and produce higher amount of NH3 compared to grass. On the basis of OM degraded, the grass yielded higher volume of gas than that of legume. The two types of feeds did not change the pH of rumen fluid. Key Words: Grass, Shrub Legume, Gas And Ammonia Production, Organic Matter Digestibility

Nutritional quality of Stenotaphrum secundatum and Brachiaria humidicola for goats Ginting, Simon P; Tarigan, Andi
Indonesian Journal of Animal and Veterinary Sciences Vol 11, No 4 (2006)
Publisher : Indonesian Animal Sciences Society

The study was aimed to evaluate several nutritional quality parameters (chemical composition, intake and digestibility) in goats of S. secundatum and B. humidicola known to be highly and moderately shade tolerant species. The species of Pennisetum purpureuphoides was used as a control diet. These forages were offered to 21 male Kacang goats with an average body weight of 15.2 kg. The animals were divided into three groups based on the body weight, and were randomly allocated to one of the three forages species in a Completely Randomized Design. During the adaptation period (14 days) and the intake monitoring period (5 days) after the adaptation period the forages were offered ad lib. During the feces and urine collection period (7 days) feed was offered at 90% of the maximumÂ intake to minimize the intake fluctuation. The dry matter content of S. secundatum (332 g kg-1DM) and B. humidicola (321 g kg-1DM) were relatively higher than P. purpureuphoides (211 g kg-1DM), while the organic matter content were relatively equal among the three forages species. The crude protein content of S. secundatum (83 g kg-1DM) and B. humidicola (85 g kg-1DM) was almost identical, but were lower than that of P. purpureuphoides (111 83 g kg-1 DM) The NDF and ADF contents were relatively similar among the forage species. The dry matter (DM) intake of goats fed S. secundatum (588 g d-1) or fed B. humidicola (577 g d-1) did not differ (P>0.05), but it was higher (P>0.05) than dry matterÂ intake of goats fed P. purpreuuphoides. The similar trend was shown when DM intake was expressed in the percentage of body weight (3.60%, 3.55% and 2.77% BW, respectively) or when expressed as gram per kg BW0.75 which were 35.5, 35.6 and 27.7 g/kg BW0.75 respectively. The digestion coefficient of DM and OM were not different (P>0.05) between S.secundatum and B. humidicola, but it was higher than that in P. purpureuphoides. The digestion coefficient of energy were not different among these forages. The N balances were positive (3,0â3,6 g d-1) in goats offered these forages. It is concluded that the nutritional qualities of S. secundatum and B. humidicola were comparable, and it is relatively higher that the P. purpureuphoides when offered to goats as the sole feed. Key Words: Nutritional Quality, Grass Species, Goats

Responses of milk quality to roasted soybeans, calcium soap and organic mineral supplementation in dairy cattle diets ., Adawiah; Sutardi, Toha; Toharmat, Toto; Manalu, Wasmen; ., Nahrowi
Indonesian Journal of Animal and Veterinary Sciences Vol 11, No 4 (2006)
Publisher : Indonesian Animal Sciences Society

Milk quality is affected by feed nutrient either macronutrient or micronutrient.Â Roasted soayabeans and calcium soap were to increase supply by pas protein and fat to dairy cattle. Thus, organic mineral was to increase bioavailability of feed mineral to animal.Â The objective of this study was to evaluate roasted soybean, mineral soap and organic mineral supplementation on milk quality of dairy cattle.Â Twenty lactating Frisian Holstein cows (initial weight 361.4 Â± 40.39 kg) were assigned into a randomized complete block design with 5 treatments and 4 blocks. The treatments were A: basal diet, B: A + roasted soybean, C: B + calcium soap of corn oil, D: C + calcium soap of corn oil, E: C + calcium soap of fish oil. The experimental diets were offered for 9 and 2 weeks preliminary. The results of the experiment showed that milk protein and lactose were not affected by diets.Â Milk dry matterÂ of cows fed A, B, and D diets were higher (P<0.05) than those of fed C and E diets.Â Milk fat of cows fed A,Â B and D diets were higher (P<0.05) than those ofÂ fed C and E diets. Milk density of cows fed B and E diets were higher (p<0.05) than those of fed A, C and D diets. Milk TPC of cows fed B diet were higher (0.05) than those of fed A, C, D, and E diets. It is concluded that milk quality especially milk protein and lactose concentration are not affected by roasted soyabeans, Ca-soap, and organic mineral. Calcium soap of fish oil and organic mineral decrease population of milk bacteria. Key Words: Calcium Soap, Organic Mineral, Roasted Soyabeans, Dairy Cattle, Quality Milk

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Ahmadi Riyanto

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Abstract

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Home Page

OAI Link

Editorial Team

Contact

Reviewer

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Contact Name Ahmadi Riyanto

Contact Email medpub@litbang.deptan.go.id

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Journal Mail Official ahmadi_puslitbangnak@yahoo.com

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Location Kota bogor, Jawa barat INDONESIA

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Contact Name
Ahmadi Riyanto

Contact Email
medpub@litbang.deptan.go.id

Phone
-

Journal Mail Official
ahmadi_puslitbangnak@yahoo.com

Editorial Address
-

Location
Kota bogor,

Jawa barat

INDONESIA