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Jurnal Ilmu Ternak Veteriner

JITV
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Published by Indonesian Center for Animal Research and Development

ISSN : 08537380 EISSN : 2252696X DOI : -

Core Subject : Health,

Veterinary

Aims JITV (Jurnal Ilmu ternak dan Veteriner) or Indonesian Journal of Animal and Veterinary Sciences (IJAVS) aims to publish original research results and reviews on farm tropical animals such as cattle, buffaloes, sheep, goats, pigs, horses, poultry, as well as non domesticated Indonesian endemic animals, such as deers, anoa, babirusa, etc. Scope Indonesian Journal of Animal and Veterinary Sciences . The journal will consider primary research papers from any source if they make an original contribution to the experimental or theoretical understanding and application of theories and methodologies of some aspects of animal science and veterinary

Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Hewan dan Zoologi

Articles 1,756 Documents

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Role of seminal plasma in maintaining quality of ram epididymal spermatozoa preserved at low temperature (3â5oC) ., Herdis; Aku, Achmad Selamet; Rizal, Muhammad; Surachman, Maman
Indonesian Journal of Animal and Veterinary Sciences Vol 11, No 4 (2006)
Publisher : Indonesian Animal Sciences Society

Cauda epididymal spermatozoa could be used as an alternative source of gamete in the application of various reproductive technologies, because the spermatozoa is motile and has ability of fertilizing the oocyte. The purpose of this research was to examine the effect of addition of seminal plasma on the quality of ram cauda epididymal spermatozoa preserved at 3â5oC. Collected-spermatozoa was divided into five parts then centrifuged at 3,000 rpm for 30 min and the supernatant was removed. One-fifth was diluted with Tris extender (Tris) and the remaining was added 0.5 ml ram seminal plasma then diluted with Tris extender (PS-Tris), 15% AndroMed + 85% distilled water (PS-AM15), 20% AndroMed + 80% distilled water (PS-AM20), and 25% AndroMed + 75% distilled water (PS-AM25), respectively. Quality of collected-spermatozoa including spermatozoa concentration (SC), percentages of motile spermatozoa (MS), live spermatozoa (LS), abnormal spermatozoa (AS), cytoplasmic droplet (CD), and intact plasma membrane (IPM) were evaluated. Percentages of MS, LS, and IPM of diluted-spermatozoa was evaluated every day during preserved at 3â5oC for three days. Results of this study showed that mean SC, MS, LS, AS, CD, and IPM of fresh spermatozoa were 11,660 million/ml, 65, 81, 7.6, 10.2, and 82.2%, respectively. Addition of seminal plasma in cauda epididymal spermatozoa prior to dilution could maintain the quality of spermatozoa during preserved at 3â5oC for three days. At day-4 of storage, percentages of MS, LS, and IPM for PS-Tris (43, 58, and 59.2%), PS-AM20 (40.5, 53.75, and 53.75%), and PS-AM25 (40, 54.8, and 55.2%) were significantly (P<0.05) higher than Tris (21, 34.8, and 33.6%) and PS-AM15 (20, 40, and 42.2%). In conclusion, addition of seminal plasma in ram cauda epididymal spermatozoa prior to dilution with Tris or 20 and 25% AndroMed extenders could maintain the quality of spermatozoa during preserved at 3â5oC for three days, and could be used for artificial insemination or in vitro embryo production programs. Key Words: Cauda Epididymal Spermatozoa, Seminal Plasma, Tris, AndroMed, Ram

The effect of incubation time on capacitation and acrosome reaction of in vitro ovine spermatozoa Wattimena, J
Indonesian Journal of Animal and Veterinary Sciences Vol 11, No 4 (2006)
Publisher : Indonesian Animal Sciences Society

The aim of this research was to know the effect of incubation time on capacitation and acrosome reaction of in vitro ovine spermatozoa. Twelve ejaculates from two Garut sheep were collected using artificial vagina and then evaluated macro and microscopically. After semen washing (centrifugation method), semen was diluted in Brackett and Oliphant (BO) medium and then incubated during 6 hours in incubator CO2, temperature 38.5ÂºC. Evaluation of capacitation and acrosome reaction was conducted on 0 hour, 0.5 hour, 1 hour, 2 hours, 4 hours and 6 hours incubation time. The result showed that incubation time had significant (P<0.05) effect on no capacitation sperm (pattern F), capacitation (pattern B) nad acrosome reaction (pattern AR). Incubation time had significantly (P<0.01) effect on subjective motility, life sperm and membrane integrity. Key Words: Ovine spermatozoa, incubation, capacitation, reaction, in vitro

The relative superiority of milk production of Garut sheep and its crossbred Inounu, I; Sukmawati, S; Noor, R.R
Indonesian Journal of Animal and Veterinary Sciences Vol 11, No 4 (2006)
Publisher : Indonesian Animal Sciences Society

Garut sheep (GG) is one of some native sheep that had been well known to the people of West Java. This sheep have some advantages including their ability to produce multiple birth, reach sexual maturity faster and resistant to internal parasite. However, this sheep have also some disadvantages including low milk production, high mortality and low weaning weight. Crossbreeding is one way to improve animal genetic quality. The objective of this study is to evaluate whether the Garut, St.Croix cross (HG), and Moulton Charollais cross (MG) are superior in milk production when compared to Garut sheep. This study was conducted at small ruminant experimental station of Research Institute for Animal production from June to August 2002.Â The superiority of the crossed sheep was determined by subtracting the average milk production of the crossed sheep (HG or MG) and Garut sheep and then divided the values by the average milk production of Garut sheep, except for the threeway crosses (MHG and HMG) is calculated from the difference in milk production between the means of threeway crossbred with the means of two parents (MG and HG). The data had been corrected by parity and type of birth.Â The General Linear model of SAS was used to calculate the least square means. Average milk production from GG, MG, HG, MHG and HMG ewes in this study were respectivelly 53.41, 59.48, 55.89, 44.87 and 54.66 kg. The relative superiority for milk production of MG and HG were 11% and 5% over Garut sheep respectively and for the threeway crossbred MHG/HMGÂ was -14% over their parents (MG and HG). Key Words: Milk Production, St.Croix, Moulton Charollais, Garut, Crossing

Using follicle stimulating hormone (FSH) for superovulation in buffalo Situmorang, Polmer
Indonesian Journal of Animal and Veterinary Sciences Vol 11, No 4 (2006)
Publisher : Indonesian Animal Sciences Society

Studies of superovulation have been carried out in buffaloes and the progress has been achieved but the number of embryo collected was still small. Two studies were conducted to evaluate the use Follicle Stimulating Hormone (FSH) for superovulation in buffalo. First study, fifteen buffaloes were randomly divided into 3 groups of five buffaloes of each group and superovulated using three type of FSH (FSH-P; FSC-China and Folltropin). Hormones were given twice a day for 4 days in decreasing doses methods. First injection initiated on day 10 of estrus cycle, followed by prostaglandin on day 12 than artificially inseminated (AI) two days later. Recovery of embryo was conducted un-surgically method by flushing uterus horn on day 6 after AI. Second study, 10 buffaloes were randomly divided into 2 groups of each five buffaloes First group was superovulated with FSH followed methodology in the first study (control group) and second group were given a prime FSH on day 1 of estrus cycle before superovulation (treatment group). After 3 months the superovulation was repeated in the reverse condition where the control become treatment and the treatment become a control group. The type of FSH did not significantly affect the superovulatory response. The mean total corpus luteum (TCL), total number of embryo (TNE) and total number of viable embryo (TVE) were 6.8, 3.3 and 2.2,Â 6.2, 3.0 andÂ 2.1 andÂ 7.2, 3.6 andÂ 2.4 for FSH-P, FSH China and Folltropin respectively. A single injection of FSH significantly increased (P<0.05) the number of embryo collected. The mean TCL, TNE and TVE were 6.9, 2.8 and 2.1 and 8.2, 3.3 and 2.5 for control and treatments respectively. The peak progesterone level was significantly higher (P<0.05) in treatment group (6.8 ng/ml) than those in control (5.6 ng/ml). Repeated the superovulation after 3 months did not significantly affect the number of embryo collected. In conclusion, superovulation in buffaloes can be performed using a types of FSH and prime injection of FSH on day 1 of estrus cycle increase the number of embryo recovered. The superovulation can be conducted after 3 months. Key Words: FSH, Superovulation, Embryo

The effect on infection of irradiated Fasciola gigantica (liver flukes) on goats (Capra hircus Linn) blood values Tuasikal, Boky Jeanne; ., Suhardono
Indonesian Journal of Animal and Veterinary Sciences Vol 11, No 4 (2006)
Publisher : Indonesian Animal Sciences Society

This experiment was carried out to study the effect of infection Fasciola gigantica irradiated by using 60Co to goatâs blood values. Twenty local male goats, ten to twelve old months, 15-20 kg of body weight were used for this study. The animals were divided into 5 groups consisting of four replications. Three groups received irradiated metacercariae of Fasciola gigantica using 45, 55 and 65 Gy respectively. One group was the positive control (goats were infected by non-irradiated metacercariae) and another one was the negative control (goats were not infected by metacercariae). Each goat received 350 metacercariae. Body weight, Eosinofil, PCV and Hb were measured every weeks for 20 weeks. The result indicated that pathogenicity of irradiated Fasciola gigantica was reduced. In all parameters, amongÂ negative control group and three irradiation-groups showed non significant (P>0.05). Key Words: Fasciola gigantica, Irradiated, Eosinofil, Packed Cell Volume, Haemoglobin

Local isolate of Saccharomyces cerevisiae as biocompetitive agent of Aspergillus flavus Kusumaningtyas, Eni
Indonesian Journal of Animal and Veterinary Sciences Vol 11, No 4 (2006)
Publisher : Indonesian Animal Sciences Society

Aspergillus flavus is a toxigenic fungus that contaminates feed and influences the animal health. Saccharomyces cerevisiae can be used as a biocompetitive agent to control the contamination. The ability of local isolate of S. cerevisiae as a biocompetitive agent for A. flavus was evaluated. A. flavus (30ml) was swept on Sabouraud dextrose agar (SDA), while S. cerevisiae was swept on its left and right. Plates were incubated at 28oC for nine days. Lytic activity of S. cerevisiae was detected by pouring its suspension on the centre of the cross streaks of A. flavus. Plates were incubated at 28oC for five days. Growth inhibition of A. flavus by S. cerevisiae was determined by mixing the two fungi on Potato dextrose broth and incubated at 28oC for 24 hours. Total colony of A. flavus were then observed at incubation time ofÂ 2, 4, 6 and 24 hours by pour plates method on the SDA plates and incubated on 28oC for two days. Growth of hyphae of A. flavus sweep were inhibited with the swept of S. cerevisiae. The width of A. flavus colony treated with S. cerevisiae is narrower (3,02 cm) than that of control ( 4,60 cm). The growth of A. flavus was also inhibited on the centre of cross streak where the S. cerevisiae poured. S. cerevisiae gradually reduced the colony number of A. flavus in the mixed culture of broth fungi ie. 14 x 103 CFU/ml while colony number of control is 80 x 103 CFU/ml. Results showed that S. cerevisiae could be used as biocompetitive agent of A. flavus. Key Words: Aspergillus flavus, Saccharomyces cerevisiae, Biocompetitive agent

Improving nutrient values of solid heavy phase for corn substitute in poultry diet Sinurat, A.P; Purwadaria, T; Bintang, I.A.K; Pasaribu, T
Indonesian Journal of Animal and Veterinary Sciences Vol 12, No 2 (2007)
Publisher : Indonesian Animal Sciences Society

Solid heavy phase (SHP), a by product material of palm oil factory obtained by ceramic filtration from liquid waste could be produced approximately 2 million tons/year. The by product has a potential for substituting corn in poultry feed. A series of experiment was carried out to improve nutrient value of the SHP in order to obtain a feedstuff that can substitute corn in poultry feed. The SHP was processed by either fermentation or enzymatic process. The product was then dried and analysed for its nutrient values. Fermentation process was carried out by altering the dry matter of the substrate (40 or 50%), while enzymatic process was carried out by altering the dose and kind of enzymes used. The process that produced best nutrient values was considered for producing materials for a feeding trial. In this trial, the products were used in diet formulation to substitute 25 or 50% of the corn included in the control diet. The results showed that the fermentation processed could be conducted with dry matter of substrate at either 40 or 50%. The fermentation process significantly improved the nutrient values of the SHP as shown by decreasing the crude fibre and increasing the crude protein, amino acids and the ME value. The results also showed that the Balitnak enzyme (BS4) was optimum when added at 10 ml/kg dry matter SHP, while the commercial enzyme (EK) was optimum at level of 2 g /kg dry matter SHP. Results of feeding trial showed that 25% of corn in layer diet could be substituted with dried SHP or SHP + enzymes. This substitution tended to improve performances (egg production, egg weight and FCR) of the laying hens. Substitution of 25 or 50% corn with the fermented SHP tends to reduce the performance of the layinghens. Similar trend also occurred when 50% of the corn was substituted with the enzymaticly processed SHP. Key Words: Solid Heavy Phase, Palm Oil Waste, Fermentation, Enzymes, Laying Hens

Comparison fermentation kinetics (in vitro) of grass and shrub legume leaves: The pattern of VFA concentration, estimated CH4 and microbial biomass production Widiawati, Y; Thalib, A
Indonesian Journal of Animal and Veterinary Sciences Vol 12, No 2 (2007)
Publisher : Indonesian Animal Sciences Society

In the process of fermentation, rumen microbes normally convert major fractions of carbohydrates and proteins in a feed to useful end-products (i.e. VFA, microbial protein and B-vitamins) and some waste products (i.e. CH4 and CO2). The pattern of these end-products depend largely on the fraction contained in the feed eaten by the animal. Two types of feeds, namely grass and shrub legume, leucaena have different fraction proportions. Grass contains more fibre but less protein compared to shrub legumes. Thus in the rumen they might be fermented to produce different pattern of end products. The experiment was conducted in order to examine the pattern of VFA, CH4 and microbial protein products of the two types of feeds when fermented in the rumen. In vitro method was used to determine the pattern of these end products. Results showed that the grass produced more total VFA/mg organic matter degraded (0.0229 mM/mg vs 0.0075 mM/mg) and CH4 gasÂ (0.20 mole/mg Â vs 0.09 mole/mg) but less propionate in partial and less microbial protein (2646 g vs 2656 g ) compared to the legume. Approximately 32% less CH4 (per mg OM degraded) would be produced from leucaena compared to that produced from grass, which mean that there will be less energy loss as CH4 thus more energy for animal production. Key Words: Grass, Shrub Legume, Volatile Fatty Acids, CH4, Microbial Protein

The effects of microwave radiation on rumen degradation characteristics of barley straw cut at two different stages of maturity Natsir, Asmuddin
Indonesian Journal of Animal and Veterinary Sciences Vol 12, No 2 (2007)
Publisher : Indonesian Animal Sciences Society

A common approach for improving the nutritive value of low quality roughages and crop by-products is by pre-treatment or processing either physical, chemical, or biological treatments. Microwave radiation is one type of physical treatment that could be used to treat low quality roughages. Research was carried out to investigateÂ the effects of microwave radiation on the rumen degradation characteristics of barley straw obtained from two different stages of maturity. The experiment was run factorially based on completely randomized block design. The first factor was stage of maturity, straw cut during the soft elongation time (C1) and during the harvest time (C2). The second factor was levels of microwave radiation times (MWRT) (T0 = control, without MWR; T1 = MWR for 1 minute, T2 = MWR for 2 minutes). The results indicated nutritive values of barley straw obtained from C1 sampling time were significantly better than that obtained from the C2 sampling time in terms of a higher rumen degradation rate and a much greater total potential rumen degradability. In contrast, MWR did not have significant effects on the rate of degradation and total potential degradability of straw in the rumen. Key Words: Microwave Radiation, Barley Straw, Rumen Degradability

Viability of bovine demi embryo after splitting of fresh and frozen thawed embryo derived from in vitro embryo production Imron, M; Boediono, A; Supriatna, I
Indonesian Journal of Animal and Veterinary Sciences Vol 12, No 2 (2007)
Publisher : Indonesian Animal Sciences Society

In vivo embryo production was limited by number of donor, wide variability respond due to superovulation program and also immunoactifity of superovulation hormone (FSH). Splitting technology could be an alternative to increase the number of transferrable embryos into recipien cows. Splitting is done with cutting embryo becoming two equal pieces (called demi embrio) base on ICM orientation.Â The objective of this research was to determine the viability of demi embryo obtained from embryo splitting of fresh and frozen thawed embryo. The results showed that demi embryos which performed blastocoel reexpansion 3 hours after embryo splitting using fresh and frozen thawed embryos were 76.9 and 76.2% respectively. Base on existention of inner cell mass (ICM), the number of demi embryos developed with ICM from fresh and frozen thawed embryos were not significantly different (90.6 and 85.7% respectively. The cell number of demi embryo from fresh embryos splitting was not different compared with those from frozen thawed embryos (36.1 and 35.9 respectively). These finding indicated that embryo splitting can be applied to frozen thawed embryos with certain condition as well as fresh embryos. Key Words: In Vitro Embryo, Splitting, Demi Embryo, Cell Number

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Home Page

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Contact Name Ahmadi Riyanto

Contact Email medpub@litbang.deptan.go.id

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Location Kota bogor, Jawa barat INDONESIA

Abstract

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Contact Name
Ahmadi Riyanto

Contact Email
medpub@litbang.deptan.go.id

Phone
-

Journal Mail Official
ahmadi_puslitbangnak@yahoo.com

Editorial Address
-

Location
Kota bogor,

Jawa barat

INDONESIA