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Ahmadi Riyanto
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medpub@litbang.deptan.go.id
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INDONESIA
Jurnal Ilmu Ternak Veteriner
ISSN : 08537380     EISSN : 2252696X     DOI : -
Core Subject : Health,
Aims JITV (Jurnal Ilmu ternak dan Veteriner) or Indonesian Journal of Animal and Veterinary Sciences (IJAVS) aims to publish original research results and reviews on farm tropical animals such as cattle, buffaloes, sheep, goats, pigs, horses, poultry, as well as non domesticated Indonesian endemic animals, such as deers, anoa, babirusa, etc. Scope Indonesian Journal of Animal and Veterinary Sciences . The journal will consider primary research papers from any source if they make an original contribution to the experimental or theoretical understanding and application of theories and methodologies of some aspects of animal science and veterinary
Articles 1,756 Documents
Utilization of complete rumen modifier on sheep fed high fibrous forages. Thalib, Amlius; Widiawati, Yeni; Haryanto, Budi
Indonesian Journal of Animal and Veterinary Sciences Vol 15, No 2 (2010)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (81.744 KB) | DOI: 10.14334/jitv.v15i2.647

Abstract

A research to improve livestock productivity and lower enteric methane production on ruminant was conducted by manipulation approach on rumen system using a complete rumen modifier (CRM). An in vivo experiment was carried out using twenty four sheep ( mean weight 18 kg) which were distributed into 3 treatment groups of feed additive: I. control ( without treatment: K); II: K + CRM-LG; III: K + CRM-EL. Diet given consisted of fermented rice straw (ad libitum) + concentrate containing 16 % protein (400 g/head/day), and drinking water was given ad libitum. The experiment was conducted for 14 weeks based on completely randomized design. By the end of the experiment, animals were placed in the metabolism cages for 2 weeks (ie. 1 week for adaptation and 1 week for data collection). Rumen liquid of each treated animal was taken for the measurement of rumen characteristics. Parameter measuremed were: total gas production; gas composition of CO2 and CH4; in vitro DMD; NH3 and VFA contents; pH; bacterial and protozoal counts; consumption/ DMI; in vivo DMD; ADG and FCR. The results showed that productivity of sheep was improved by CRM treatments followed by lowered enteric methane production. The ADG values of CRM treatments (71.4 to 73.5 g) were significantly higher (P < 0.05) than that of control (50 g). The improvement of average daily gain was followed by a better feed conversion (P < 0.05) (ie. 10.6 vs. 12.8). The CRM treatments lowered the percentage of CH4 by 24% compared to Control (P < 0.05). The total and composition of VFA of CRM-treated rumen liquor were significantly different (P<0.05) compared to that of rumen liquor of Control (ie. the total VFA: 85.3 vs 73.5 mM and the percentage of acetic acid: 67.8 vs 60.3%). It is concluded that CRM treatment resulted in positive effects on growth of ruminant fed high fibrous forages such as rice straw and could lower enteric methane production. Key Words: Rumen Modifier, Productivity, Enteric Methane, Sheep, Rice Straw
In vitro slow-release urea contained in rice straw-based diets to increase efficiency of rumen microbial protein synthesis Kardaya, Dede; Wiryawan, K.G; Parakkasi, A; Winugroho, H.M
Indonesian Journal of Animal and Veterinary Sciences Vol 15, No 2 (2010)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (204.683 KB) | DOI: 10.14334/jitv.v15i2.648

Abstract

Effect of slow-release urea on efficiency of rumen microbial protein synthesis (EMPS) was examined using an in vitro technique. The objective of this experiment was to reveal the in vitro slow-release urea characteristics of zinc-urea, zeolites-urea, and zeolites-zinc-urea in relation to EMPS observed in different incubation time. The experimental design employed was randomized block design with 4 x 3 factorial plus a control treatment, and conducted in two replications. Factors were various urea sources (urea, zinc-urea, zeolites-urea, and zeolites-zinc-urea) and molasses concentrations (0%, 6%, and 12%) in rice straw-based diets. The control treatment was the rice straw-based diets containing neither urea nor molasses. Diets consisting of 45% rice straw and 55% concentrates (DM basis) were formulated to have similar N and TDN levels. Responses of parameters measured were subjected to MANOVA using the GLM procedure of SPSS 16.00 and differences among mean values, if applicable, were examined using HSD-test. Orthogonal comparisons were used to determine the effects of the control treatment vs. various urea sources. Results indicated that treatment of UZ combined with 6% of molasses showed the highest microbial biomass production (2.71 mg/l) at 24 hours fermentation period with its peak production estimation (3.2 mg/l) reached at 33.5 hours of fermentation period. Moreover, UZ treatment resulted in the highest microbial protein synthesis (1,381.45 ± 77.1 mg/l) at 24 hours fermentation period with its peak microbial protein synthesis estimation (1,756.04 mg/l) reached at 33.7 hours of fermentation period. The highest EMPS (25.98 ± 1.21 mg/100 mg OMD) was achieved when ration contained 6% of molasses. Key words: Slow-Release Urea, Microbial Protein Synthesis, Microbial Biomass, In vitro
Profile of blood glucose and ultrastucture of beta cells pancreatic islet in alloxan compound induced rats Suarsana, I Nyoman; Priosoeryanto, B.P; Bintang, M; Wresdiyati, T
Indonesian Journal of Animal and Veterinary Sciences Vol 15, No 2 (2010)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (556.762 KB) | DOI: 10.14334/jitv.v15i2.649

Abstract

Diabetes is marked by elevated levels of blood glucose, and progressive changes of the structure of pancreatic islet histopathology. The objective of this research was to analyse the glucose level and histophatological feature in pancreatic islet in alloxan compound induced rats. A total of ten male Spraque Dawley rats of 2 months old were used in this study. The rats were divided into two groups: (1) negative control group (K-), and (2) positif induced alloxan group (diabetic group =DM). The rats were induced by a single dose intraperitonial injection of alloxan compound 120 mg/kg of body weight. The treatment was conducted for 28 days. Blood glucose levels of rats were analysed at 0, 4, 7, 14, 21, and 28 days following treatment. At the end of the experiment, rats were sacrificed by cervical dislocation. Pancreas was collected for analysis of histopathological study by Immunohistochemical technique, and ultrastructural study using transmission electron microscope (TEM). The result showed that Langerhans islet of diabetic rat (rat of DM group) showed a marked reduction of size, number of Langerhans islet of diabetic rat decrease, and characterized by hyperglycemic condition. By using TEM, beta cells of DM group showed the rupture of mitochondrial membrane, the lost of cisternal structure of inner membrane of mitocondria, reduction of insulin secretory granules, linkage between cells acinar with free Langerhans islet, and the caryopicnotic of nucleus. Key words: Alloxan, Beta Cells, Rat, Blood Glucose, Immunohistochemistry
Relative superiority of Boer x Kacang goats at pre-weaning Mahmilia, Fera Mahmilia; Doloksaribu, Meruwald
Indonesian Journal of Animal and Veterinary Sciences Vol 15, No 2 (2010)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (193.063 KB) | DOI: 10.14334/jitv.v15i2.650

Abstract

productivity expected from these crosses is the effect of heterosis or hybrid vigor. Boer goat is selected to improve the productivity of local Kacang goats, because this goat has a high genetic potential and has a large body formation. The objective of this research was to evaluate relative superiority of crossbreed between Boer and local goat (Kacang) during pre weaning period. The research was conducted at Goat Research Station, Sei Putih-North Sumatra, from Januari 2007 to December 2009. During pre weaning period the kids were reared with their mothers in group pen. Research results showed that birth weight and weaning weight of crossbreds (Boerka 1 and Boerka 2) were heavier than that of Kacang goat. The relative superiority of the crossbreds were 21,89 - 43,20% for birth weight, 32,88 – 54,68% for weaning weight and 37,11 – 53,32% for pre weaning daily gain. In addition the degree of mortality at pre weaning period of crossbreeds was lower than that of Kacang goats. Key words: Kacang Goat, Boer, Boerka, Relative Superiority
Satin and their crossbred rabbit production Brahmantiyo, Bram; Raharjo, Y.C; Martojo, H; Mansjoer, S.S
Indonesian Journal of Animal and Veterinary Sciences Vol 15, No 2 (2010)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (74.916 KB) | DOI: 10.14334/jitv.v15i2.651

Abstract

Rabbit productivity in intensive management to be evaluated considering development in the rabbit farming were rapidly increased. Rex, Satin and their crossbred (Reza) rabbits have been developed in Research Institute for Animal Production and productivity information of each breed can be used as basis for policy or model of cultivation and breeding in the community. Research conducted by evaluating the productivity of growth, carcass and the carcass proportion of the three strains of rabbit. Rex and satin growth were no different, and Reza was higher at 12-14 weeks of age. Carcass production comes from cutting the rabbit at the age of six months, this trait is strongly influenced by the weight of rabbits, and Reza gives the lowest performance compared to Rex and Satin. Rex, Satin and Reza were medium type rabbits with dual-purpose product (fur and meat). These rabbits showed the childs growth is good enough, to adapt the environment temperature and high food and have adequate carcass production. Key Words: Rabbit, Rex, Satin, Reza, Growth, Carcass
Analyses of fixed effects for genetic evaluation of dairy cattle using test day records in Indonesia Anang, Asep; Indrijani, Heni; Tasripin, Didin
Indonesian Journal of Animal and Veterinary Sciences Vol 15, No 2 (2010)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (441.933 KB) | DOI: 10.14334/jitv.v15i2.652

Abstract

Season, rainfall, day of rain, temperature, humidity, year and farm are fixed effects, which have been reported to influence milk yield. Those factors are often linked together to contribute to the variation of milk production. This research is addressed to study the fixed effect factors, including lactation curve, which should be considered for genetic evaluation of milk yield based on test day records of dairy cattle. The data were taken from four different farms, which were PT. Taurus Dairy Farm, BPPT Cikole, Bandang Dairy Farm, and BBPTU Baturraden. In total of 16806 test day records were evaluated, consisting of 9,302 at first and 7,504 at second lactation, respectively. The results indicated that fixed effects were very specific and the influences had different patterns for each farm. Consequently, in a genetic evaluation, these factors such as lactation, temperature, year, day of rain, and humidity need to be evaluated first.  Ali-Schaeffer curve represented the most appropriate curve to use in the genetic evaluation of dairy cattle in Indonesia. Key words: Fixed Effects, Milk Production Curve, Test Day, Dairy Cattle
Assessment of the purity and characteristics of rat splenic T cells isolated by one-step discontinuous gradient of percoll Depamede, Sulaiman Ngongu Depamede
Indonesian Journal of Animal and Veterinary Sciences Vol 15, No 2 (2010)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (243.1 KB) | DOI: 10.14334/jitv.v15i2.653

Abstract

T cells isolated from spleen lymphocytes of rat (Rattus rattus) have been commonly used in an immunological research. However the T cells are still contaminated with other splenic cells.  Therefore a simple and reliable technique to isolate the T cells in one step process is needed. The aim of the present study was to assess whether T cells isolated from spleen lymphocytes of rat as a model by means of discontinuous density gradient of Percoll could give pure T cells with their activities as needed in an immunological research. Isolation was carried out in 5 density gradients of Percoll i.e. 1.052, 1.063, 1.075, 1.085, and 1.122 g/ml. The purity of the cell fractions was determined by immunocytochemistry and fluorescence-activated cell sorting (FACS) methods. The activity of each lymphocyte fraction was determined through cell proliferation assays by culturing the cells in the presence of phytohemaglutinin (PHA) and Concanavalin A (Con A) as T cells inducers. The FACS results show that fraction I and II were found to contain mostly B cells and macrophages. The fractions were obtained on the layers of gradient 1.052/1.063 and 1.063/1.075. Fraction III (gradient 1.075/1.085) contain T cells (43.2%) which consisted of CD4 (34.4%) and CD8 (18.6%), B cells (36.5%), and macrophages (10.0%). Fraction IV was strongly expressed T cell receptors (76.2%) which consisted of CD4 (63.6%) and CD 8 (20.0%), while B cells were found 20.0% and macrophages just about 0.6%. The activity of T cells as expressed by their respond to PHA and Con A shown that fraction III gave the most positive response, while fraction IV which contained the most ‘pure’ T cells gave less activity. From the present study it can be concluded that the discontinuous density gradient of Percoll method can be used to isolate and purify T cells in one step process. Furthermore, the present study indicated that T cells to proliferate in response to mitogen require other immune cells such as B cells and macrophages. Key words: Percoll, Lymphocyte, T Cell, Rat, Mitogen
Genetic Polymorphism of the Lactoferrin Gene in Dairy and Beef Cattles at National Artificial Insemination and Embryo Transfer Stations Anggraeni, Anneke; Mumpunie, G.E; Misrianti, R; Sumatri, C
Indonesian Journal of Animal and Veterinary Sciences Vol 17, No 4 (2012)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (387.428 KB) | DOI: 10.14334/jitv.v17i4.691

Abstract

Lactoferrin (LTF) adalah gen pengontrol komponen protein susu dan memiliki karakteristik sebagai antimikrobial.  LTF pada susu berfungsi untuk mencegah diare, sedangkan pada sapi laktasi untuk mencegah mastitis pada ambing. Mempertimbangkan peran penting dari gen LTF, maka perlu dilakukan peningkatan kadar LTF dalam susu melalui seleksi pada taraf DNA. Polymorfisme genetik dari gen LTF diidentifikasi pada sapi perah dan potong dengan metoda Polymerase Chain Reaction - Restricsion Fragment Length Polymorphism (PCR-RFLP), dengan enzim restriksi EcoRI. Genotyping dilakukan pada sapi perah Friesian Holstein (FH) total sejumlah 89 ekor, meliputi dari Balai Inseminasi Buatan Lembang (BIB Lembang) untuk 17 pejantan, Balai Besar IB Singosari (BBIB Singosari) untuk 32 pejantan, dan Balai embrio Transfer Cipelang (BET  Cipelang) pada 40 dara. Genotyping dilakukan pula pada sapi potong dara berasal dari empat bangsa, meliputi Limousin (14 ekor), Angus (5 ekor), Simmental (13 ekor) dan Brahman (5 ekor) dari BET Cipelang. Gen LTF|EcoRI pada sapi perah dan potong pengamatan menghasilkan dua tipe alel, yaitu alel A dan B. Kedua jenis sapi tersebut menghasilkan hanya dua genotipe, yaitu  genotipe AA dan AB, tanpa genotipe BB. Ini dapat menjadi hal yang baik karena genotipe AA dan AB dipertimbangkan berasosiasi dengan ketahan pada mastitis. Nilai-nilai dari heterozygositas observasi (Ho) dari gen ini lebih tinggi dibandingkan heterozigositas ekspektasi (He). Disimpulkan bahwa gen LTF|EcoRI memiliki variasi yang baik pada sapi perah dan sapi potong dari ketiga balai bibit nasional tersebut. Kata Kunci: Polymorfisme genetik, Friesian Holstein, gen Lactoferrin, PCR-RFLP
Identification of Single Nucleotide Polymorphism (SNP) in Mono Amine Oxidase A (MAO-A) Gene as a genetic marker for aggressiveness in sheep Handiwirawan, Eko; Noor, Ronny R; Sumantri, C; ., Subandriyo; Inounu, I
Indonesian Journal of Animal and Veterinary Sciences Vol 17, No 4 (2012)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14334/jitv.v17i4.692

Abstract

In the population, there are aggressive sheep in a small number which requires special management those specific animal house and routine management. The purpose of this study was to identify the variation of DNA marker SNP (single nucleotide polymorphism) as a genetic marker for the aggressive trait in several of sheep breed. The identification of point mutations in exon 8 of MAO-A gene associated with aggressive behavior in sheep may be further useful to become of DNA markers for the aggressive trait in sheep. Five of sheep breed were used, i.e.: Barbados Black belly Cross sheep (BC), Composite Garut (KG), Local Garut (LG), Composite Sumatra (KS) and St. Cross Croix (SC). Duration of ten behavior traits, blood serotonin concentrations and DNA sequence of exon 8 of MAO-A gene from the sheep aggressive and nonaggressive were observed. PROC GLM of SAS Ver. 9.0 program was used to analyze variable behavior and blood serotonin concentrations. DNA polymorphism in exon 8 of MAO-A gene was analyzed using the MEGA software Ver. 4.0. The results show that the percentage of the aggressive rams of each breed was less than 10 percent; except for the KS sheep is higher (23%).  Based on the duration of behavior, aggressive sheep group was not significantly different with non aggressive sheep group, except duration of care giving and drinking behavior. It is known that concentration of blood serotonin in aggressive and non aggressive rams was not significantly different. The aggressive trait in sheep has a mechanism or a different cause like that occurs in mice and humans. In this study, aggressive behavior in sheep was not associated with a mutation in exon 8 of MAO-A gene. Key Words: Sheep, Aggressive, Exon 8 Of MAO-A Gene, Single Nucleotide Polymorphism
Morphological characteristics of forage crops indigofera zellongeriana at different levels drought stress and interval pruning Herdiawan, Iwan; Abdullah, L; Sopandie, D; Karti, P.D.M.H; Hidayati, N
Indonesian Journal of Animal and Veterinary Sciences Vol 17, No 4 (2012)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (128.263 KB) | DOI: 10.14334/jitv.v17i4.693

Abstract

The objectives of this experiment was to evaluate the effect of drought stress and defoliation interval on shoot and root morphologic characteristic of Indigofera zollingeriana. The experiment was arranged in a completely randomized design with two factors and four replications. The first factor consisted of 3 level of drought stress i.e: 100% field capacity (FC) (control), 50% FC, and 25% FC. The second factor comprised of 3 defoliation interval were interval defoliations of 60, 90 and 120 days. The observed variables were shoot, root dry weight, root/shoot ratio and root length. Data were analyzed by ANOVA and the differences between treatments were tested by LSD. The results shows that there were interactions (P < 0,05) between drought stress and defoliations interval on shoot dry weight, while root dry weight, root/shoot ratio, and root length was not. Drought treatment significantly (P < 0,05) decreased shoot, root dry weight, but increase of root/shoot ratio and root length. Defoliation interval significantly affected (P < 0,05) on shoot dry weight, but not on root dry weight, root/shoot ratio, and root length. Key Words: Indigofera zollingeriana, Drought Stress, Defoliation Interval, Shoot And Root Morphology

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