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Jurnal Ilmu Ternak Veteriner

JITV
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Published by Indonesian Center for Animal Research and Development

ISSN : 08537380 EISSN : 2252696X DOI : -

Core Subject : Health,

Veterinary

Aims JITV (Jurnal Ilmu ternak dan Veteriner) or Indonesian Journal of Animal and Veterinary Sciences (IJAVS) aims to publish original research results and reviews on farm tropical animals such as cattle, buffaloes, sheep, goats, pigs, horses, poultry, as well as non domesticated Indonesian endemic animals, such as deers, anoa, babirusa, etc. Scope Indonesian Journal of Animal and Veterinary Sciences . The journal will consider primary research papers from any source if they make an original contribution to the experimental or theoretical understanding and application of theories and methodologies of some aspects of animal science and veterinary

Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Hewan dan Zoologi

Articles 1,756 Documents

88 89 90 91 92

Reliable preservation methods of mitochondrial DNA analysis for larvae and adult flies of Chrysomya bezziana (Diptera: Calliphoridae). April H Wardhana; S Muharsini; Suhardono .
Jurnal Ilmu Ternak dan Veteriner Vol 8, No 4 (2003): DECEMBER 2003
Publisher : Indonesian Center for Animal Research and Development (ICARD)

The Old World Screwworm fly, Chrysomya bezziana is known as a myiasis agent in Indonesia. The aim of the study is to establish a reliable preservation methods for molecular analysis of insect. Cytochrom b (CB) and Cytochrome Oxidase sub unit I (COI) of mitochondrial DNA were used as markers. Drying method was used to preserve adult flies, while the larvae were kept in 10% formalin and 80% ethanol with or without soaked using hot water prior preservation. Two samples of each preservation were tested. Four primers were used to amplify samples using Polymerase Chain Reaction (PCR). The PCR product were then purified and sequenced. Sequence data analysis of all samples suggested no differences between all preservation methods except using 10% formalin. Preservation samples using 10% formalin is not suitable for collecting mitochondrial DNA of insect. The best methods was using 80% ethanol with killed using hot water prior preservation. This method was able to extract a stabile mitochondrial DNA. Key words: Chrysomya bezziana, preservation, formalin, ethanol, mitochondrial DNA

Effects of zinc and calcium minerals intake on cadmium accumulation in the liver of broiler chicken Sri Rachmawati; Darmono .; Zainal Arifin
Jurnal Ilmu Ternak dan Veteriner Vol 4, No 3 (1999): SEPTEMBER 1999
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Cadmium is a heavy metal which can accumulate in animal tissues, may have an effect the growth of chicken, causes a residual problems and is danger for human consumption. Study on evaluating the effect of mineral zinc (Zn) and calcium (Ca) intake on cadmium (Cd ) accumulation in the liver of broilers and their growth was carried out. One hundred and eighty of day old chicks (DOC) were divided into 6 groups, group I was used as control, group II, III, IV, V and VI were given feed containing of 5 ppm Cd, 5 ppm Cd and 10 ppm Zn, 5 ppm Cd and 15 ppm Zn, 5 ppm Cd and 10 ppm Ca, 5 ppm Cd and 15 ppm Ca respectively in their feed. Cd, Zn and Ca were added as CdCl2, ZnCl2 and CaCl2. During the experiment, at week -0, -1,-2,-3 and -4, five chickens from each group were weighed and then they were slaughtered and livers were collected. The liver samples were analyzed for Cd content by Atomic Absorption Spectrophotometer. The result indicated that Cd exposed to chickens caused the accumulation of that Cd in their liver. The addition of 15 ppm Zn in feed reduced the accumulation of Cd in liver significantly (P<0.05). However the addition of Ca in chicken feed gave greater effect in reducing the Cd accumulation in liver compared to that of Zn supplementation. 10 ppm Ca and 15 ppm Ca in chicken feed caused the accumulation of Cd in liver chicken significantly decreased (P<0.05). Ca supplementation also caused of increasing body weight of chickens. The body weight of five weeks old chickens given 15 ppm Ca in their feed as in average of 1,820 grams is higher than that of chickens in control group, which was 1,761 grams. Whereas the body weight of chickens in group IV, which was given with 15 ppm Zn in their diet was in average of 1,745 grams. It was concluded that Zn and Ca can reduce the accumulation of Cd in broiler chicken liver. Key words : Zinc, calcium, accumulation, cadmium

The effect on infection of irradiated Fasciola gigantica (liver flukes) on goats (Capra hircus Linn) blood values Boky Jeanne Tuasikal; Suhardono .
Jurnal Ilmu Ternak dan Veteriner Vol 11, No 4 (2006): DECEMBER 2006
Publisher : Indonesian Center for Animal Research and Development (ICARD)

This experiment was carried out to study the effect of infection Fasciola gigantica irradiated by using 60Co to goat’s blood values. Twenty local male goats, ten to twelve old months, 15-20 kg of body weight were used for this study. The animals were divided into 5 groups consisting of four replications. Three groups received irradiated metacercariae of Fasciola gigantica using 45, 55 and 65 Gy respectively. One group was the positive control (goats were infected by non-irradiated metacercariae) and another one was the negative control (goats were not infected by metacercariae). Each goat received 350 metacercariae. Body weight, Eosinofil, PCV and Hb were measured every weeks for 20 weeks. The result indicated that pathogenicity of irradiated Fasciola gigantica was reduced. In all parameters, among negative control group and three irradiation-groups showed non significant (P>0.05). Key Words: Fasciola gigantica, Irradiated, Eosinofil, Packed Cell Volume, Haemoglobin

Dermatopathology of Caprine Scabies and Protective Immunity in Sensitised Goats Against Sarcoptes scabiei Reinfestation Simson Tarigan
Jurnal Ilmu Ternak dan Veteriner Vol 7, No 4 (2002): DECEMBER 2002
Publisher : Indonesian Center for Animal Research and Development (ICARD)

The purpose of this study was to compare macroscopic dermatopathology in naïve and sensitised goats, and to assess protective immunity possessed by sensitised goats against Sarcoptes scabiei challenge. Eighteen goats were allocated evenly into 3 groups; group 1 sensitised with the mite twice, group 2 once and group 3 was not sensitised (naïve). Sensitisation was done by infesting goats with the mites on the auricle and infestation was allowed to progress for 7 weeks, then the goats were treated with Ivermectin to obtain complete recovery. After sensitisation, all sensitised and naïve goats were infested with the mites on the auricles. Infestation in the sensitised goat caused severe immediate hypersensitivity that resulted in severe peracute pustular dermatitis. After one week, however, the lesion waned slowly. At 7 weeks post infestation, the remnant of lesion could only be perceived by palpation on the primary site of infestation as a mild papular dermatitis. Infestation on the naïve goats, in contrast, produced slowly progressing lesions which at 7-week post infestation, it ended up with severe crusted scabies affecting almost the whole skin. Antigens responsible for the immediate hypersensitivity which are supposedly contained in the mite secretions or excretions are immunologically protective but unlikely to have the capacity to induce a complete protection against mite challenge in immunised animals. This notion is based on the fact obtained from this study that goats sensitised twice did not possess a higher immune protection against mite challenge than goats sensitised once. Key words: Sarcoptes scabiei var. caprae, sensitisation, protective immunity, immediate hypersensitive

Ability of fiber bacteria isolated from buffalo rumen in digesting various sources of forage Iwan Prihantoro; T Toharmat; D Evvyernie; Suryani .; L Abdullah
Jurnal Ilmu Ternak dan Veteriner Vol 17, No 3 (2012): SEPTEMBER 2012
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Local buffalo rumen fluid is a source of fiber digesting bacteria. Such bacteria presumably are well adapted to feed stuffs derived from agricultural byproducts with low quality. The purposes of this study were: (1) to isolate the fiber-digesting bacteria from buffalo rumen fluid, (2) to determine the fiber digesting characteristics and adaptability of such bacteria, and (3) to characterize the bacterial diversity. Rumen fluids for the experiment were collected from buffaloes slaughtered at the Faculty of Animal Science, Bogor Agricultural University abattoir. Fiber-digesting bacteria were isolated using McDougall buffer supplemented with fibrous feed. A factorial experiment was conducted to study characteristics and adaptability of the bacteria using two factors: the type of bacteria and type of feed source of the fiber. Diversity among the bacteria was analyzed using the NTSys2.10 program.Results showed that nine bacterial isolates had a high adaptability to fiber feed based on CMCase. The highest CMCase activity bacteria for Pennisetum purpureum were A9 (11.36±1.70 unit/ml/h), A3 (11.22±0.60 unit/ml/h) and A42 (10.62±1.96 unit/ml/h). CMCase activity of fiber digesting bacteria from buffalo rumen fluid was not correlated with the number of bacteria in the culture. Based on genetic similarity, nine isolates were grouped into five types having similarity≥46%. Key Words: Buffalo Rumen Fluid, Fiber-Digesting Bacteria, Low Quality Forage

Comparative study on preferences and quality of poultry meat. Triyantini .; Abubakar .; I.A.K Bintang; T Antawidjaja
Jurnal Ilmu Ternak dan Veteriner Vol 2, No 3 (1997)
Publisher : Indonesian Center for Animal Research and Development (ICARD)

A study was conducted to examine physical and nutritional compositions as well as carcass preferences of broilers, native chickens, ducks and muscovy using 25 birds from each species . Parameters measured were : percentage of carcass, part of carcass and by-products, carcass quality, tenderness, water, protein, fat and ash contents of meat and skin . Preference test was conducted on thigh and breast meat, as well as whole carcass and cuts. The results of the study showed that the carcass quality of native chicken and ducks were better than the carcass of broilers and muscovy, and over 80% of them were considered as first class quality . The carcass percentage of broilers was higher than the native chickens, ducks and muscovy (P < 0 .01) . The tenderness of breast meat was not significantly different, (36 .16-40 .84 kg/second), however, the tenderness of broiler thigh meat was the highest (61 .77 kg/second) among other meat (P < 0 .01). Protein content of duck breast meat was the lowest, while the protein content of duck thigh meat and the muscovy were higher than that of broilers and native chickens (P < 0 .05) . Fat content of broilers thigh meat was the highest as compared to other poultry meats (P < 0 .01) . Organoleptic test showed that the taste of breast and thigh meat of native chicken, duck and muscovy were similar with that of broilers, however the appearance and color of carcass of broilers and muscovy were more accepted than that of native chickens and ducks (P < 0.05). The meat of native chickens, ducks and muscovy were considered as good protein sources as judged by the physical appearances, nutritional composition and the preferences test . Keywords : Poultry meat, quality, preference

Effect of mixing banana stem juice and subsequent heating on rumen degradability of soy bean meal D Yulistiani; W Puastuti; I.W Mathius
Jurnal Ilmu Ternak dan Veteriner Vol 15, No 1 (2010): MARCH 2010
Publisher : Indonesian Center for Animal Research and Development (ICARD)

The study was conducted to evaluate the rumen degradability of soy bean meal when it was mixed with banana stem juice and was subsequently heated. Banana stem juice was used as source of tanin to protect protein in soy bean meal from degradation in the rumen. The banana stem juice (in volume) was mixed with soy bean meal at three ratio, which were 1 : 1, 1:2 and 1 : 3 (w/v). These mixing was then heated at 60oC and 90oC. The heating was done in an oven. Sample from each treatment was then filled into polyester bag with porosity of 45 mm and the size of 5 x 9 cm. The bags were incubated in the rumen of fistulated sheep for 0, 2, 4, 8, 16, 24 and 48 hours. The experiment was carried out in factorial 3 x 2, with three levels of banana stem juice and 2 levels of heating and was arranged in completely randomized design. Untreated soy bean meal was also incubated used as control. Degradation data of dry matter and crude protein at each incubation time was fitted into exponential equation, P = a + b (1 - ect). Results shows that there was a significant interaction factor of stem juice ratio and heating on water soluble substrate (constant A) where heating at 90oC with ratio 1 : 2 (w/v) resulted in the highest a. The insoluble but fermentable substrate (B) was decreased by the increasing concentration of banana stem juice, while the rate of protein degradation of b fraction was lower compared to control. In conclusion, banana stem juice can reduce the rate of degradation of soy bean meal in the rumen. Key words: Degradation, Crude Protein, Soy Bean Meal, Banana Stem Juice

Study of effectiveness of bacteria isolated from rumen microbes with acetogen medium as methanogenesis inhibitor Amlius Thalib; Y Widiawati; H Hamid
Jurnal Ilmu Ternak dan Veteriner Vol 9, No 4 (2004): DECEMBER 2004
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Ruminal methanogenesis has disadvantageous effects on ruminant animals and environment of atmosphere. Inhibition of methane produced through reduction of carbondioxide has been conducted by bacteriological approach. The approach involved the isolation of bacteria from rumens of sheep (IBD) and buffalo (IBK) using medium for CO-utilizing acetogens. The isolate of bacterium was multiplied with the usual culture medium and then used as inoculum to degrade a substrate of King grass under constant temperature (390C) for 48 hours. Fresh rumen fluid of sheep (CRDS) was used as comparing inoculum. Measurementswere carbondioxide and methane gasses, pH, volatile fatty acid (VFA) and NH3-N contents, bacterial count, and dry matter digestibility (in vitro DMD). The data measured were analyzed by using completely randomized design. The results showed that morphological cell of IBD was oval pleomorphic with Gram negative type, and cell of IBK was rod with Gram negative type. Percentage of CH4 produced by inoculum of IBD was lower than CRDS but was not significantly different (29.47 vs. 33.07%), while the percentage of methane produced by inoculum of IBK was very significantly lower than CRDS (24.29 vs. 33.07%) (P<0.01). Acetate/propionate ratio as a result of substrate fermentation by inoculum of IBD (3.55) and IBK (3.79) were very significantly higher than that of CRDS (2.43) (P<0.01). It is concluded from this experiment that isolates used were effective to inhibit the methanogenesis and the species contained in the isolates were indicated to be homoacetogenic bacteria. Key words: Bacterial isolate, acetogen medium, methanogenesis, inhibitor

cDNA library construction and isolation of genes for candidate vaccine antigens from Chrysomya bezziana (the Old World Screwworm fly) Tony Voucolo; Florentina Supriyanti; Sri Muharsini; Gene Wijffe
Jurnal Ilmu Ternak dan Veteriner Vol 5, No 3 (2000): SEPTEMBER 2000
Publisher : Indonesian Center for Animal Research and Development (ICARD)

The construction and use of cDNA libraries for the isolation of genes encoding candidate antigens for use in a recombinant vaccine against Chrysomya bezziana is described. RNA was isolated and mRNA purified from first and third instar larvae of Chrysomya bezziana and used in the synthesis of two cDNA libraries in the bacteriophage vector λ ZAP express®. These libraries were screened using Digoxigenin-labeled DNA probes obtained from two independent approaches. First, a homolog approach used probes designed from previously characterized peritrophic membrane genes identified from the related myiasis fly, Lucilia cuprina. Secondly, a de novo approach used amino-terminal and internal peptide sequence information derived from purified Chrysomya bezziana peritrophic membrane proteins to generate DNA probes. Three peritrophic membrane genes were identified and characterized. Chrysomya bezziana peritrophin-48 was identified using the homolog approach and, Chrysomya bezziana peritrophin-15 and Chrysomya bezziana peritrophin-42 were identified using the de novo approach. The identification of these genes as encoding candidate antigens against Chrysomya bezziana has allowed the production of recombinant proteins for use in vaccination trials. Key words: cDNA library, peritrophin, peritrophic membrane, Chrysomya bezziana, Lucilia cuprina, vaccine

Genetic diversity of Lombok chickens based on D-loop mitochondrial DNA sequences M. Syamsul Arifin Zein; Sri Sulandari
Jurnal Ilmu Ternak dan Veteriner Vol 13, No 4 (2008): DECEMBER 2008
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Mitochondrial DNA (mtDNA) displacement (D)-loop sequences were used to study the genetic diversity and relationship of Lombok chickens. A total of 45 individuals were sampled. The D-loop segment was PCR amplified and subsequently sequenced. The sequences of the 785 nucleotides were used for analysis. Twelve haplotypes were identified from 25 polymorphic sites with polymorphism between nucleotides 200 and 400 contributing to 80% of the variation. Fu’s Fs value was - 8.768 (all samples, P = 0), indicating high genetic diversity and population expansion, a conclusion supported by a neighbor– joining analysis of the haplotypes. Nucleotides diversity of the Lombok chicken were 0.00221 and haplotype diversity were 0.654 + 0.08. The dominant haplotype found among the Lombok chickens was haplotype B (62%) and genetic distances value ranged from 0.001 to 0.017. Key Words: Mtdna, D-Loop, Genetic Diversity, Haplotype, Lombok Chicken

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Home Page

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Contact Name Ahmadi Riyanto

Contact Email medpub@litbang.deptan.go.id

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Location Kota bogor, Jawa barat INDONESIA

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Contact Name
Ahmadi Riyanto

Contact Email
medpub@litbang.deptan.go.id

Phone
-

Journal Mail Official
ahmadi_puslitbangnak@yahoo.com

Editorial Address
-

Location
Kota bogor,

Jawa barat

INDONESIA