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Jurnal Ilmu Ternak Veteriner

JITV
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Published by Indonesian Center for Animal Research and Development

ISSN : 08537380 EISSN : 2252696X DOI : -

Core Subject : Health,

Veterinary

Aims JITV (Jurnal Ilmu ternak dan Veteriner) or Indonesian Journal of Animal and Veterinary Sciences (IJAVS) aims to publish original research results and reviews on farm tropical animals such as cattle, buffaloes, sheep, goats, pigs, horses, poultry, as well as non domesticated Indonesian endemic animals, such as deers, anoa, babirusa, etc. Scope Indonesian Journal of Animal and Veterinary Sciences . The journal will consider primary research papers from any source if they make an original contribution to the experimental or theoretical understanding and application of theories and methodologies of some aspects of animal science and veterinary

Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Hewan dan Zoologi

Articles 1,756 Documents

89 90 91 92 93

Detection of Mycobacterium avium subspecies paratuberculosis of dairy cows in Bogor Widagdo Sri Nugroho; M. Sudarwanto; D.W Lukman; E. Setiyaningsih; E. Usleber
Jurnal Ilmu Ternak dan Veteriner Vol 14, No 4 (2009): DECEMBER 2009
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Johne’s disease (JD) or partuberculosis is a chronic granulomatous enteritis in ruminants caused by infection of Mycobacterium avium paratuberculosis subspecies (MAP). The disease has been detected serologically in Indonesia. It’s potential to spread to other herds and could create great economic losses. The objectives of current study were to detect MAP in milk and faeces of dairy cows as well as to evaluate the association between farm management factors and presence of the bacteria in dairy cows in Bogor. The sample size was calculated using the formula to detect disease with the prevalence assumed to be 5% using 95% significant level. Milk and faeces samples were taken from 62 dairy cows which were suspected as suffering from MAP infection. Detection of MAP was done by isolation in Herrold’ egg yolk medium with mycobactin J (HEYMj), acid-fast bacilli Ziehl-Neelsen staining, PCR IS900 and F57. Biochemical test to confirm M. tuberculosis presence was also conducted. Fifteen isolates of Mycobacterium sp. were found from the faeces samples but not from the corresponding milk samples. However, conventional PCR conducted on the isolate as well as the milk samples, gave negative results. Biochemical test proved that all Mycobacterium sp. isolates were not M. tuberculosis. This study indicated the prevalence of MAP in Bogor was less than 5%. These findings should be continued by observational study to achieve the comprehensive information at the cattle and herd level. Bovine Tuberculosis monitoring should be done also to protect dairy herd and food safety for the community. Key words: Johne’s disease, Mycobacterium avium subspecies paratuberculosis, Milk, Faeces

Prototype of A/Duck/Sukoharjo/Bbvw-1428-9/2012 subtipe H5N1 clade 2.3.2 as vaccine on local duck Risa Indriani; NLPI Dharmayanti
Jurnal Ilmu Ternak dan Veteriner Vol 19, No 2 (2014): JUNE 2014
Publisher : Indonesian Center for Animal Research and Development (ICARD)

A/Duck/Sukoharjo/Bbvw-1428-9/2012 virus subtipe H5N1 clade 2.3.2 as seed vaccine on local duck. AI H5N1 clade 2.3.2 vaccine containing 256 HAU per dose was formulated using adjuvant ISA 71VG Montanide ™. Six groups of one day old local duck were used in this study. Three groups (10 ducks per group) were vaccinated and 3 groups (9 duck per group) were served control. Vaccination was conducted when the duck were three weeks old of age using single dose. Three weeks after vaccination when the duck were challenged either with HPAI H5N1 clade 2.3.2, or HPAI H5N1 clade 2.1.3 virus at dose 106 EID50/ 0.1 ml by drops intranasaly. Result showed that vaccination produced 100% protection compared to unvaccinated ducks againt HPAI subtipe H5N1 clade 2.3.2, and 100% protection againt HPAI H5N1 clade 2.1.3 (A/ck/wj/Subang-29/2007 and A/ck/wj/Smi-Part/2006), while unvaccinated ducks showed virus shedding on day 3 post infection. Key Words: Duck, Influenza, Clade 2.3.2, Vaccine, Clade 2.1.3

The susceptibility differences of buffalo and Ongole calves against trickle infection with Fasciola gigantica Ening Wiedosari; S Widjajanti; S Partoutomo
Jurnal Ilmu Ternak dan Veteriner Vol 3, No 1 (1998)
Publisher : Indonesian Center for Animal Research and Development (ICARD)

A pen trial was carried out in order to determine the susceptibility differences of a trickle infection with Fasciola gigantica in buffalo and Ongole calves. Treated animals were infected orally with 15 metacercariae of F. gigantica twice weekly for 32 weeks and were slaughtered at 36 weeks. The results showed that buffalo calves had significantly lower fluke burdens than Ongole calves (P<0 .01) . All of the infected Ongole calves had fasciola eggs in their faeces 18 weeks after the commencement of infection. In contrast, eggs were detected only in 3 out of 7 infected buffalo calves at week 20, in 4 at week 28 and in 6 at week 30. Faeces of the seventh buffalo remained free of eggs until week 36. Rates of growth were reduced by 25%and 10,3% in infected Ongole and buffalo calves respectively . Circulating blood eosinophilia ofboth hosts, ahallmark of helminth infections, increased following infection, but values in buffaloes was greater than Ongole calves mainly in week 4 and 8 after infection (P<0.01). These results might be concluded that the susceptibility of buffalo calves to trickle infection with F. gigantica was lower compared to Ongole calves. Keywords : Fasciola gigantica; susceptibility, buffalo calves, Ongole calves

Effect of temperature and time of pasteurization on the milk quality during storage Abubakar .; Triyantini .; R Sunarlim; H Setiyanto; Nurjannah .
Jurnal Ilmu Ternak dan Veteriner Vol 6, No 1 (2001): MARCH 2001
Publisher : Indonesian Center for Animal Research and Development (ICARD)

A study on the effect of temperature and time of pasteurization on the milk quality during storage was carried out using fresh milk. The aim of the experiment was to asses the storage time of pasteurized milk for consumption without nutrient losses. A completely randomized factorial design, 2 x 8 was used, with pasteurization temperature (T), consisted of 2 levels, the low temperature long time (LTLT), i.e. fresh milk was warmed at 65oC for 30 minutes (T1) and the high temperature short time (HTST), i.e. fresh milk was warmed at 71oC for 15 seconds (T2); and storage time (S), consisted of 8 levels, i.e. 0, 3, 6, 9, 12, 15, 18, and 21 hours respectively, as the factors, with 3 replicates. Parameters measured were alcohol test, water, fat, and protein concentrations, and microbial population of pasteurized milk during storage. Data were analyzed using analysis of variance and simple linear regression. The result showed that water and fat concentrations and microbial population was not significantly different (P>0.05) in pasteurization temperature treatment, but was significantly different (P<0.05) due to storage time treatment. Meanwhile, the protein concentration was significantly different (P<0.05) either in pasteurization temperature or storage time. It was concluded that pasteurized milk was still suitable for consumption at 15-21 hours storage, while protein concentration tended to be better when was pasteurized at 65oC. Key words: Pasteurized milk, storage quality

Study of erysipelas vaccine: Immunogenicity of three culture fractions of E. rhusiopathiae serotype 2 local isolate on swine Siti Chotiah
Jurnal Ilmu Ternak dan Veteriner Vol 13, No 1 (2008): MARCH 2008
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Erysipelothrix rhusiopathiae as the causal agent of erysipelas was isolated from swine in Indonesia. The aims of this study were to develop a local isolate vaccine for erysipelas and determine its immunogenicity in swine. Three types of fractionated culture of local isolate E. rhusiopathiae serotype 2 consisting of whole culture (WCu), whole cell (WCe) and culture filtrate (CF) were made into an oil adjuvant at a final bacteria concentration about 1010 colony forming unit (CFU). Four groups of swine (12 swines/group) were vaccinated intra muscularly with WCu, WCe and CF containing 2 ml per dose respectively, and K as a control group without vaccination. The vaccine was injected twice with three weeks interval. All groups were challenged with local isolates of E. rhusiopathiae; serotype 1 (heterologous) and serotype 2 (homologous) at five weeks after second vaccination except CF group was challenged at twelve weeks after the second vaccination by 107.5 CFU. The specific antibody responses were monitored every two weeks by ELISA technique, and protective responses were determined by clinical signs of erythema urticaria or mortality during the observation for 14 days. The results showed that the antibody responses of WCu, WCe, and CF groups against extract antigen were not different. The protective responses of groups WCu, WCe, CF, and K against challenged local isolate of homologous serotype and heterologous were 83.3, 83.3, 91.7 and 0% respectively, with average value of optical density 0.492, 0.454, 0.529 and 0.3450 respectively, at the time of challenge. Cross protection occurred on all culture fractions tested. In conclusion, the three culture fractions of local isolate of E. rhusiopathiae serotype 2 containing protective antigen and could be used for vaccine candidate in Indonesia. Key Words: Erysipelothrix rhusiopathiae, Local Isolate, Immunogenicity, Swine, Vaccine

Performance of broiler chicken fed physically and chemically treated jatropha (Jatropha curcas) seed meal Tiurma Pasaribu; E. Wina; B. Tangendjaja; S. Iskandar
Jurnal Ilmu Ternak dan Veteriner Vol 14, No 1 (2009): MARCH 2009
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Jatropha seed meal which is a by-product of biofuel is rich in protein. Its utilization as feed ingredient is limited by the presence of several anti nutritive and toxic compounds. An experiment was conducted at the Indonesian Research Institute for Animal Production to evaluate the effect of jatropha seed meal on broiler performance. Jatropha seed meals were treated physically, chemically or their combination to reduce or eliminate the anti nutritive and toxic compounds. Then, the inclusion of untreated or treated jatropha seed meals in the diet at the level of 4% was evaluated on broiler. The experiment was done in Completely Randomized Design with 5 treatments of feed, i.e 1) control feed, 2) feed containing untreated jatropha seed meal (OO), 3) feed containing physically treated jatropha seed meal (OTO), 4) feed containing chemically treated jatropha seed meal (EHM) and 5) feed containing physically and chemically treated jatropha seed meal (EHMO). Every treatment had 7 replications with 5 chickens for each replication. The treatment diets were given to 7 days old chicken for 14 days. The observed parameter were feed intake, daily gain, feed conversion ratio, and mortality. The broiler performance recieved EHMO feed was better that those of OTO or EHM treatment (751.1; 731.2; 498.8 g of body weight for EHMO, EHM and OTO treatments, respectively), however, it was lower than the control treatment (856.3 g). Feed Conversion Ratios of EHM and EHMO treatments were not significantly different from control treatment (1.868; 1.813 vs 1.707), however, they lower than OO (2.532) and OTO (2.249) treatments. Chicken mortality of EHMO treatment was much lower than OO that of treatment (0 vs 34.29%, respectively). In conclusion, the processing technology of jatropha seed meal using combined physical and chemical treatments (EHMO) was the best choice as it gave better chicken performance without mortality compared to other techniques in this experiment. Key words: Jatropha Seed Meal, Detoxification, Broiler Chicken, Performance

Protection of inactive intranasal ántrax vaccine to Bacillus anthracis infection Adin Priadi; Lily Natalia; Rahmat S. Adji
Jurnal Ilmu Ternak dan Veteriner Vol 15, No 2 (2010): JUNE 2010
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Ánthrax is an endemic zoonotic disease distributed in many parts of Indonesia. Although vaccination program has been implemented in many areas, cases are still frequently reported. Farmers are reluctant to vaccinate their livestock since spore vaccine used in the field often cause side effects and death of the animals. To overcome this problem, an inactive vaccine composes of Bacillus anthracis toxins, cell wall and capsule subunits was developed. B. anthracis Sterne strain (34F2) was selected to produce toxins and cell walls. Local Bacillus anthracis isolated from Citaringgul was used to produce capsule as the Polymerase Chain Reaction (PCR) revealed that this isolate poses cap gene encoding for capsule. Two vaccines compose of 15 μg toxoid, 30 μg of capsule, 15 μg of cell wall and 30 μg toxoid, 60 μg of capsule, 15 μg of cell walls were designated as vaccine I and vaccine II respectively. For each experiment, 10 mice were nasally immunized by placing 5 μl of vaccine into each nare 3 times at 2-week intervals. A group of 10 mice were unvaccinated and used as control. Blood was collected fortnightly to monitor antibody responses. All mice were challenged with 2 x 105 B. anthracis Sterne spores injected subcutaneously two weeks after the last vaccination. Two weeks after vaccination of antibodies to B. anthracis toxin, capsule and cell wall were detected in dot-blot assay. Mice that were immunised intranasally with chitosan adjuvanted vaccine developed high IgG responses in sera as detected by ELISA, and the response was dose dependent. Vaccine II gave better response than vaccine I. Vaccine I and II protected mice from challenge at a rate of 60 and 80% respectively. This results showed that intranasal B. anthracis vaccine composes of toxin, capsule and cell wall with chitosan as an adjuvant gave a good protection against B. anthracis Sterne spores challenge in mice. Key Words: Inactive Intranasal Ántrax Vaccine, Protection, Bacillus anthracis, Mice

Extension program on the control of bovine fasciolosis in West Java, Indonesia Eny Martindah; A Kusumaningsih; S Widjajanti; S Partoutomo; B Frank; Suhardono .
Jurnal Ilmu Ternak dan Veteriner Vol 3, No 3 (1998)
Publisher : Indonesian Center for Animal Research and Development (ICARD)

An extension program to control fasciolosis in cattle and buffalo was undertaken in collaboration with officers of the District of Livestock Services (DLS) and farmer organizations in the Surade district of West Java. Control strategies were based on results of extensive epidemiological studies on fasciolosis in this area over the past 4 years. Recommendations included: (1) preventing animals grazing harvested rice fields adjacent to a village or cattle pen; (2) feeding stock only the top two-thirds of freshly cut rice stalks; (3) mixing cattle or buffalo faeces with manure of ducks or chicken naturally infected with Echinostoma revolutum, before using them as fertilizer in rice fields; and (4) a single treatment with triclabendazole in July, about 6 weeks after harvest of the last seasonal rice crop. Farmers were surveyed in January 1996 to determine their level of knowledge about fasciolosis. The extension program commenced in February, soon after planting the second seasonal rice crop in four villages. At first, leaflets were distributed to farmers, and posters were displayed in each village to provide basic information. Following discussions with village leaders, groups of farmers met in each village to discuss the advantages they saw in each strategy, ways they could implement them, and to identify socio-economic constraints that needed to be overcome. Taped interviews were prepared for a local radio station and the farmer groups. In August, final survey was conducted to determine the change in knowledge and attitudes that had occurred as a result of the extension program. Bennett’s hierarchy was used at each stage to evaluate the effects of inputs and activities. Farmers adopted the techniques of cutting and feeding rice-stems 2/3 above water-level, and isolating cattle from rice-fields during harvest time, as these appeared to be beneficial in social and economic terms; but they rejected the two other practices where they perceived that socio-economic costs exceededbenefits. Key words : Fasciolosis control, extension program, cattle, buffalo

Pre-and postcalving supplementation of multinutrient blocks on lactation and reproductive performances of grazing Bali cows H.L.L Belli
Jurnal Ilmu Ternak dan Veteriner Vol 11, No 1 (2006): MARCH 2006
Publisher : Indonesian Center for Animal Research and Development (ICARD)

The influence of multinutrient blocks during pre and postcalving on lactation and reproductive performances of Bali cows were evaluated. Seventeen multiparous pregnant cows with body condition score (BCS) 1 to 2, approximately 90 d before the expected date of calving, were divided randomly into groups A (n=9) and B (n=8), and were grazed on the native pasture as a basal diet, while those of Group B received 1.25 kg multinutrient blocks, whose constituent was as follows (%): molasses (28), urea (5), coconut cake (15), fishmeal (5), rice bran (25), lime (8.5), salt (7.5), grit (5) and ultramineral (1). Cows were weighed and assessed for BCS (on a five-point scale) every two weeks, commencing at 12 weeks prior to calving, within 24 h after calving up to 16 weeks after calving. Milk production and composition were assessed by the weigh-suckle-weigh technique at four times i.e. 2, 4, 8 and 12 weeks after calving. Suckling behaviour i.e. frequency of suckling, duration of nursing and total min nursed were observed 6 times in the course of lactation at weekly intervals commencing at 1 week after calving. Calf birth weight was measured within 24 h after calving and continued at weekly intervals until 12 weeks of age. Uterine involution was determined by rectal palpation at 7 d postcalving. The interval from calving to first estrus was monitored by estrus observation twice a day. Conception at first service was assessed by pregnancy diagnosis 45 to 60 d after insemination. Cows fed multinutrient blocks supplement had higher liveweight, BCS throughout the experiment. The cows produced significantly more milk and had higher growth rates of the calves than the unsupplemented cows. The mean values of the characteristics of suckling were influenced by supplementation. The rate of uterine involution and conception to first service were similar in the two treatment groups, but interval from calving to the exhibition of the first estrus was shorter in supplemented cows. Key Words: Supplementation, Multinutrient Block, Bali Cows

The content of melamine in milk based products, and milk powders analyzed by liquid chromatography mass spectrometry Rachmawati S; Widiyanti PM
Jurnal Ilmu Ternak dan Veteriner Vol 18, No 1 (2013): MARCH 2013
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Melamine is a white crystal of organic compound has a molecular weight of 126.12, difficult to solve in water. Cases of illegally adding melamine in milk powder is subjected to increase the nitrogen content of milk, because melamine contains high nitrogen (66%), so when milk is tested, seems contains high protein. This paper presented data the content of melamine in products based milk, and milk powders which entered and marketed in Indonesia. Melamine analysis is done by LC- MS 2010 EV, Shimadzu. Confirmation and validation tests indicate that melamine scanning found at m/z = 127, suitable system of analysis with relative standard deviation (RSD) given of 1,18% (< 2,0%). Accuracy test gave the average of 89.1% recovery, detection limit of 5 ppb and limit of quantition 7 ppb. About 91.3% samples (n = 46) collected from animal quarantine Tanjung Priok contained melamine in the range of 6.7 ppb to 61.5 ppb which is 1/49 to 1/16 times less than standard limit. Whereas about 40%, 14 out of 35 samples collected from Bandung and Jakarta supermarket was not detected of melamine, and 60% samples positive contain melamine in the range of 5,1 to 26,5 ppb (1/200 to 1/38 standard limit). However, all the samples analyzed contain melamine below the standard limit of 1 ppm determined by WHO/FAO. Key Words: Melamine, Milk Powder, Milk Based Products, LCMS

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Location Kota bogor, Jawa barat INDONESIA

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Ahmadi Riyanto

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medpub@litbang.deptan.go.id

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Journal Mail Official
ahmadi_puslitbangnak@yahoo.com

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Location
Kota bogor,

Jawa barat

INDONESIA