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Aktivitas Antibakteri Ekstrak Etanol Porifera (Spongia Officinalis ) terhadap Staphylococcus Aureus ATCC 25923 Indria Hafizah; Fine Farhani Muliati; Sulastrianah Sulastrianah
MEDULA JURNAL ILMIAH FAKULLTAS KEDOKTERAN UNIVERSITAS HALU OLEO Vol 4, No 1 (2016)
Publisher : Halu Oleo University

Staphylococcus aureus is the most dangerous of all of the many comman staphylococcal bacteria. It stains gram positive and is non moving small round shaped or non motile cocci..Porifera (Spongia officinalis) are lowly organised less evelved animals and can be used as the medication resources. The aim of this study was to inveftigate antibacterial extract etanol of Porifera (Spongia officinalis)against the growth of Staphylococcus aureus ATCC 25923.Present study was an experimental in vitro test. Antibacterial activity assays were carried out by the disc diffusion method. Antibacterial activity was determined by the clear zone formed around the papaer disc and minimum inhibitor concentration which could inhibit the growth of Staphylococcus aureus ATCC 25923.The results are supported by the presence of secondary metabolite by phytochemical test such as alkaloid, terpenoid, steroid, saponin and tanin.Extract ethanolof Spongia officinalis. Extract etanol Spongia officinalishas antibacterial activity againts Staphylococcus aureus ATCC 25923. Minimum inhibitory concentration from the ekstrak etanol Spongia officinalis was 10 ppm.Conclution ; Extract etanol Spongia officinalishas antibacterial activity againts Staphylococcus aureus ATCC 25923Keywords: Spongia officinalis, Staphylococcus aureus, Antibacterial activities, MIC (Minimal Inhibitory Consentration)

Analisis Faktor Risiko Obesitas dan Hipertensi dengan Kejadian Diabetes Melitus Tipe 2 di Rumah Sakit Umum Daerah Kota Kendari Asmarani Asmarani; Andi Cahaya Tahir; Anisa Adryani
MEDULA JURNAL ILMIAH FAKULLTAS KEDOKTERAN UNIVERSITAS HALU OLEO Vol 4, No 2 (2017)
Publisher : Halu Oleo University

ABSTRACTBackground:Type-2 Diabetes Mellitus is one type of diabetes that is the highest rates of incidence. Type-2 DM is a metabolic disorder with a characteristics of chronic hyperglycemia that occurs due to insulin resistance or lack of insulin secretion. Based on basic medical research showed that prevalence of type-2 DM in Indonesia keep on Increasing. Increasing cases of diabetes are theoretically cannot be separated from the risk factors that affect it. Purposes:This research aims to determine whether obesity and hypertension is a risk factor in the occurrence of type-2 DM. Methods:This research is observational analytic research with case-control matching design. The population were all patients who visited the RSUD Kota Kendari in September-December 2016. The sampling method is purposive sampling with the cases are those patient who was diagnosed with type-2 DM and the control are not. The samples are 136 people with the comparison cases and controls is 1:1 so that the number of cases-samples are 68 people and controls-samples are 68 people who had been matched such as age and gender. The instrument of this research are the weight-scales and microtoice to assess the nutritional status with calculation of BMI and also stethoscope and sphygmomanometer to measure the blood pressure. Data were analyzed by determining the odds ratio. Data was considered significant if UL and LL does not include the value of 1. Result:The results of this research showed that obesity and hypertension are a risk factor for the occurrence of type-2 DM with the results of statistical tests of obesity obtained OR : 7,164., CI 95% : 3,365-15,250 and hypertension obtained OR : 4,166., CI 95% : 2,026-8,567. Conclusion: The conclusions of this research is that obesity and hypertension are a risk factor in the incidence of type-2 DM in RSUD Kota Kendari in 2016.Keywords: Type-2 Diabetes Mellitus, Obesity, Hypertension

Hubungan Kadar Timbal dalam Darah terhadap Kejadian Hipertensi pada Operator SPBU di Kota Kendari Noviarsih Muslimah; Hartati Hartati; Fedelia Raya
MEDULA JURNAL ILMIAH FAKULLTAS KEDOKTERAN UNIVERSITAS HALU OLEO Vol 4, No 2 (2017)
Publisher : Halu Oleo University

ABSTRACTHypertension is a common health problem and often asymptomatic until advanced stages of development and often leads to death. One of the factors that play a role in the occurrence of hypertension is a heavy metal, that is lead (Pb) which is used as an additional chemical mixture of gasoline. Gas station’s operator is one of the jobs are high-risk exposure to lead for a long time. This study aims to determine the association of blood lead levels with incidence of hypertension among the gas station’s operators in Kendari. This study used cross sectional design with observational analytic approach. The study was conducted at nine stations in Kendari and Forensic and Molecular Biology Laboratory, Faculty of Mathematics and Science, Haluoleo University, Kendari on 16 - 20 December 2016. Sample consisted of 30 operators of gas station, using proportional stratified sampling technique. The instrument used in this study were questionnaires and testing blood specimens in the laboratory by ashing method. Analysis of the data used in this study was Fisher Exact Test. The result showed respondents with normal blood lead levels are 6 respondents (20.0%) and abnormal blood lead levels are 24 respondents (80.0%). Respondents who have hypertension are 26 respondents (86.7%) and respondents who did not have hypertension are 4 respondents (13.3%). Respondents with normal lead levels who had hypertension totaled 2 respondents (6.7%) and who do not have hypertension totaled 4 respondents (13.3%). Respondents with abnormal lead levels who had hypertension totaled 24 respondents (100.0%) and who do not have hypertension numbered 0 respondents (0.0%). Based on the result of data analysis using the Fisher Exact statistical tests on the correlation of blood lead levels in the incidence of hypertension among the gas station’s operators in Kendari, p value = 0.001 which means that H0 is rejected. There is correlation between blood lead level with incidence of hypertension among the gas station’s operators in Kendari .Keywords: Lead in the blood, hypertension, gas station’s operator

Evaluasi Rasionalitas Penggunaan Obat Ditinjau dari Indikator Peresepan Menurut World Health Organization (WHO) di Seluruh Puskesmas Kota Kendari Tahun 2016 Sunandar Ihsan; Sabarudin Sabarudin; Mesi Leorita; Andi Sitti Zaenab Syukriadi; Merlyn H. Ibrahim
MEDULA JURNAL ILMIAH FAKULLTAS KEDOKTERAN UNIVERSITAS HALU OLEO Vol 5, No 1 (2017)
Publisher : Halu Oleo University

ABSTRACTBackground: Puskesmas is a first level of health facility in the public health services and for that, rationality drug used is important to achieve a better quality of life supporting productivity and nationality endurance. Aim: This study aim to evaluated rationality of drug used thorough WHO prescribing indicator to all subdistrict public health (Puskesmas) in Kendari City. Method: This was a cross sectional observational study at 15 subdistrict public health service with restrospective data collected. Total sample was 1680 prescription for 2016 period. Data compare with WHO standard indicator. Result: Result of this study showed that average drug prescriber was 3.23, average percentace of medicine prescribed by generic name was 96.08%, average percentage encounters with antibiotic prescribed was 36.85%, average percentage encounters with injection prescribed was very low 0.16%, average percentage of medicine with National Formularium was 75.07%. Result showed that drug used at all Puskesmas was still irrational except injection used and there was significant difference (p<0.005) between Puskesmas except injection used. There were three Puskesmas (Puskesmas Labibia, Puskesmas Kemaraya, and Puskesmas Benu-benua) has achieved standard of percentage of drug prescribed. Antibiotic achieved standard used was Puskesmas Nambo, Puskesmas Benu-benua, Puskesmas Wua-wua, Puskesmas Labibia and Puskesmas Perumnas. There were no one Puskesmas has achieved generic names and national formularium prescribed.Conclusion: Puskesmas Benubenua was most rational drug use with prescribing indicator of WHO.Keywords: Rational Drug Use, Prescribing Indicator, Puskesmas, Kendari City

Uji Aktivitas Antioksidan Mikroalga Air Tawar Rio Kristian; Sapto Raharjo; Sulastrianah Sulastrianah
MEDULA JURNAL ILMIAH FAKULLTAS KEDOKTERAN UNIVERSITAS HALU OLEO Vol 3, No 1 (2015)
Publisher : Halu Oleo University

Cell or tissue damage due to oxidative stress can be slowed by giving antioxidants. Antioxidants are vital substances that may help protect the body against free radicals by neutralizing or mitigate negative impacts. The human body is naturally equipped antioxidant defenses. but these antioxidants can not completely prevent cell damage. Body still requires antioxidants from the outside. The purpose of this study was to determine the antioxidant potential of microalgae species in freshwater.This research is a descriptive study of the identification of species of freshwater microalgae samples taken with a microscope and testing of extracts from freshwater microalgae species of antioxidant activity using DPPH method. Microalgae extract as much 2,25ml then homogenized with DPPH as 0,75ml in containers with a volume of 3ml. The containers are then incubated for 30 minutes in a dark room. Furthermore, the container is inserted into the spectrophotometer to read absorbance at 519nm wavelength. Results absorbance at the input curves to obtain IC50 values.The results that have identified three species of freshwater microalgae namely Navicula sp., Oscillatoria sp., And Carteria sp. From all three species showed strong inhibition against DPPH with IC50 values sequentially 41.304 ppm, 23.401 ppm and 51.433 ppm. The conclusions of this study are species of freshwater microalgae are obtained, namely Navicula sp., Oscilatoria sp., And Carteria sp. has potential antioxidant activityKeywords: Microalgae, antioxidant, DPPH

Korelasi Antara Pola Makan, Cara Menggosok Gigi, Pengetahuan dan Sikap dengan Status Karies Gigi Pada Siswa Sekolah Menengah Pertama Riszki Riszki; Sulastrianah Sulastrianah
MEDULA JURNAL ILMIAH FAKULLTAS KEDOKTERAN UNIVERSITAS HALU OLEO Vol 2, No 2 (2015)
Publisher : Halu Oleo University

Dental Caries is one of the most common mouth disease in children. According to the data obtained from Public Health Centre in North Buton regency, since 2011 to 2013 there were 304, 141 and 167 cases of dental caries respectively in Kulisusu District, placing the dental caries at the eigth rank. The aim of this study is to determine the correlation between dietary habit, way of teeth brushing, knowledge and attitude in dental health with dental caries status at SMPN 4 Kulisusu in North Buton regency in 2014. The design of this study is analytic observational with cross sectional approach. The population was consist of 98 students and the samples were obtained by total sampling method. A questionnaire was distributed to measure dietary habit, way of teeth brushing, knowledge and attitude. The Dental caries status was obtained using DMF-T Index.The data analyzed by conducting Somer’s test at the significance level 0,05. The result of this study showed that 60,2% respondent have poor dietary habit, 68,4% respondent not brushing their teeth with good manner, 39,8% respondent do not have good knowledge about dental health and 51,0% respondent do not have good attitude in dental care.According to DMF-T index, 10,2% have very high index, 25,5% have high index, 37,8%, have moderate index, 18,4% have low index and 8,1% have very low index. The conclusions of the study showed that there were statistically significant relationships between dietary habit, way of teeth brushing and attitude with dental caries status (p = 0,001, 0,000, and 0,031 respectively). Knowledge has no statistically significant relationship with dental caries status (p-Value = 0,867).Keywords: dental caries status, dietary habit, way of teeth brushing, knowledge, attitude, Junior high School

Perbandingan Metode Proporsi dengan Metode Resazurin Microtiter Assay (Rema) untuk Deteksi Mycobacterium tuberculosis yang Resisten Terhadap Rifampisin Yenti Purnamasari; Sartini Sartini; Faisal Attamimi; Muh. Nasrum Massi
MEDULA JURNAL ILMIAH FAKULLTAS KEDOKTERAN UNIVERSITAS HALU OLEO Vol 2, No 2 (2015)
Publisher : Halu Oleo University

Tuberculosis (TB) is one of the high infectious diseases in Indonesia caused by Mycobacteriumtuberculosis. Drugs which used for tuberculosis therapy has been widely reported experiencing resistance, while inappropriately treatment will lead to not effective and efficient care. Culture in Lowenstein Jensen medium is a gold standard to perform the drug susceptibility but it takes a long time to get results, therefore, new methods were developed to replace it, one of them is colorimetric method. This research aimed to compare Rifampicin susceptibility test in colorimetric method use REMA and culture method use Lowenstein Jensen medium. This study was a cross sectional with experimental laboratory design used 42 sputums from patients with tuberculosis, done at October 2010 until March 2011. In this research, 5 samples rifampicin resistant have been detected. As a results, we founded Rifampicin susceptibility test of REMA have 80 % sensitivity, 100 % specificity, 100 % PPV, and 97 % NPV when compared with culture method.Keywords:Proportion, Resazurin, Mycobacterium tuberculosis, Resistance

Analisis Faktor Risiko Ketuban Pecah Dini di Rumah Sakit Umum Bahteramas Heny Hastuti; I Putu Sudayasa; Juminten Saimin
MEDULA JURNAL ILMIAH FAKULLTAS KEDOKTERAN UNIVERSITAS HALU OLEO Vol 3, No 2 (2016)
Publisher : Halu Oleo University

Premature rupture of membrane (PROM) is an important issue in the obstetric-related difficulty premature birth and the occurrence of infections which increases morbidity and perinatal mortality and cause an infection in the mother, therefore treatment PROM requires action that is detailed so that it can decrease the incidence of labor prematuritas and infection in the uterus. This research aims to analyze the risk factors associated with the incidence of PROM in RSU Bahteramas. This type of research is observational analytic study with nested case control design. This research was conducted in RSU Bahteramas and on research using secondary data, namely medical record of the patient. The sample in this research is the mother who experienced PROM and sample control on research this is a mother who experienced the birth normal delivery. Total sample of 178 sample consisting of 89sample cases and 89 the sample control. The data were analyzed using Odds Ratio test. The results showed that maternal age is a risk factor for the incidence of PROM with OR = 4.95 (2,52-9.72; 95%), maternal parity is a risk factors with OR = 9.94 (4.44-22, 24; 95%), education level is a risk factors with OR = 2.43 (1,32-4.49; 95%) and Gemelli was not a risk factor of PROM with the OR = 2.61 (7.77-0.88; 95%).Maternal age, Maternal Parity and education level is a risk factors in the incidence of PROM and gemelli is not a risk factor for incident PROM in RSU Bahteramas from January 2013 – December 2014.Keywords: PROM, Maternal age, maternal parity, education level and Gemelli.

Uji Daya Hambat Fraksi Rumput Laut Merah Kappaphycus sp. terhadap Pertumbuhan Bakteri Staphylococcus aureus Sufiah Asri Mulyawati; Yusmiati Yusmiati; Amiruddin Eso
MEDULA JURNAL ILMIAH FAKULLTAS KEDOKTERAN UNIVERSITAS HALU OLEO Vol 4, No 1 (2016)
Publisher : Halu Oleo University

Background:Staphylococcus aureus is gram positive bacteria can cause skin infections, pericarditis, osteomyelitis, septic arthritis, endocarditis and toxic shock syndrome. S. aureus common infection cause and found massively to be resistant to some antibiotics. Kappaphycus sp. is one type of red seaweed that has an bioactive compounds as antibacterial activity. Research Purposes:The purpose of this research determine the fraction of red seaweed Kappaphycus sp. to inhibition the growth of bacteria staphylococcus aureusResearch Methods:this research used experimental of design post test control only. Samples were obtained from the sub Tononggeu, Abeli. Inhibition test using disc diffusion method of various types of concentrations of fractions. Minimum inhibitory concentration (MIC) is determined by the lowest concentration that could inhibit the growth of bacteria. Positive control cefadroxil and negative control DMSO 10%. Research result:The result of this research shown that the fraction of n-hexane and ethyl acetate red seaweed Kappaphycus sp. has activity against bacterial growth Staphylococcus aureus seen with the clear zone. Diameter of clear zone obtained in n-hexane fraction is 27,3 mm (4000 ppm), 23,3 mm (2000 ppm), 22,3 mm (1000 ppm), 4,3 mm (500 ppm), 2 mm (250 ppm) and is 0 mm (125 ppm), while the fraction of ethyl acetate 24,6 mm (4000 ppm), 22 mm (2000 ppm), 19,6 mm (1000 ppm), 4,3 mm (500 ppm), 1,3 mm (250 ppm) and 0 mm (125 ppm). MIC of n-hexane fraction was 250 ppm with a diameter of 2 mm, and the ethyl acetate fraction was 250 ppm with a diameter of 1,3 mm. Conclusion:The conclusion of this research is the fraction of n-hexane and ethyl acetate fraction red seaweed Kappaphycus sp. has particulary inhibitory effect on bacterial growth staphylococcus aureusKeywords: Inhibition, Kappaphycus sp., antibacterial, Staphylococcus aureus.

Efek Larvasida Ekstrak Biji Buah Pepaya (Carica papaya L.) terhadap Larva Instar III Aedes aegypti L. Arimaswati Arimaswati; La Ode Muhammad Sawaluddin; Hittah Wahi Sudrajat
MEDULA JURNAL ILMIAH FAKULLTAS KEDOKTERAN UNIVERSITAS HALU OLEO Vol 4, No 2 (2017)
Publisher : Halu Oleo University

ABSTRACTThe larvacide Effects of Pawpaw’s Seed Extract (Carica papaya L) Againts Third Instar Larvae of Aedes aegypti L. (Biolarvacide Alternative Study). The aim of this research was to determine the Larvacide Effects of Pawpaw’s Seed Extract (C.papaya L) towards Third Instar Larvae of A. aegypti L. The research was conducted at the laboratory of Biology Education Department and laboratory of Chemistry Education Department, FKIP Halu Oleo University. Independent Variable (X) in the research is Pawpaw’s Seed Extract (C.papaya L) with concentration level are control or was not gived extract (X0), 0.125% (X1), 0.250% (X2), 0.375% (X3), 0.500% (X4), and 0.625% (X5) and Dependent variable (Y) is the mortality of Third Instar Larvae of Aedes aegypti L. The method in the research was used experimental method with Completely Randomized Design (CRD), 3 times repetitions, each group containing 20 larvae, so the total of sample were 360 larvae. The data analyze tecnic was used descriptive analyze to knowing the larvacide effect of Seed Extract (Carica papaya L) against the mortality of Third Instar Larvae of Aedes aegypti L., varians analyze and BJND test with a significant value 95% (α = 0.05) and probit analyze to calculate LC50value or lethal concentration 50%. The result of the descriptive analyzed shows in the control group there are not mortality of larvae. The highest concentration Pawpaw’s Seed Extract (Carica papaya L) was gived mortality of larvae is 0.625% (X5) or 100% (20 larvae) and low concentration is 0.125% (X1) or 45% (9 larvae). The result of sidik ragam analyze showing that the value of Fhitung> Ftabel, there was a significant influence of the Pawpaw’s Seed Extract (Carica papaya L) for the mortality of Third Instar Larvae of Aedes aegypti L.The result of probit analyzed is LC50 value was gived 50% mortality of larvae is 0.154%.Keywords :Pawpaw’s Seed Extract (Carica papaya L), Larvacide, Third Instar Larvae of Aedes aegypti L.

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Sulastrianah

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sulastrianahmuhtadi@gmail.com

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+6285242541601

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sulastrianahmuhtadi@gmail.com

Editorial Address
Kampus Hijau Bumi Tridharma Anduonohu Kendari Universitas Halu Oleo Fakultas Kedokteran

Location
Kota kendari,

Sulawesi tenggara

INDONESIA

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Home Page

OAI Link

Editorial Team

Contact

Reviewer

Google Scholar

Contact Name Sulastrianah

Contact Email sulastrianahmuhtadi@gmail.com

Phone +6285242541601

Journal Mail Official sulastrianahmuhtadi@gmail.com

Editorial Address Kampus Hijau Bumi Tridharma Anduonohu Kendari Universitas Halu Oleo Fakultas Kedokteran

Location Kota kendari, Sulawesi tenggara INDONESIA

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Contact Name
Sulastrianah

Contact Email
sulastrianahmuhtadi@gmail.com

Phone
+6285242541601

Journal Mail Official
sulastrianahmuhtadi@gmail.com

Editorial Address
Kampus Hijau Bumi Tridharma Anduonohu Kendari Universitas Halu Oleo Fakultas Kedokteran

Location
Kota kendari,

Sulawesi tenggara

INDONESIA