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INDONESIA
Indonesian Journal of Biotechnology
Published by Universitas Gadjah Mada
ISSN : 08538654     EISSN : 20892241     DOI : -
Core Subject : Science,
Biochemistry, Genetics & Molecular Biology Immunology & microbiology Materials Science & Nanotechnology
The Indonesian Journal of Biotechnology (IJBiotech) is an open access, peer-reviewed, multidisciplinary journal dedicated to the publication of novel research in all aspects of biotechnology, with particular attention paid to the exploration and development of natural products derived from tropical—and especially Indonesian—biodiversity. IJBiotech is published biannually and accepts original research articles featuring well-designed studies with clearly analyzed and logically interpreted results. A strong preference is given to research that has the potential to make significant contributions to both the field of biotechnology and society in general.
Arjuna Subject : -
Articles 530 Documents
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Enzyme and hormone activities related to phosphorus uptake limitation in oil palm (Elaeis guineensis Jacq.) Maryanto, Sigit Dwi; Rohman, Randi Abdur; Roberdi, Roberdi; Nuringtyas, Tri Rini; Purnomo, Purnomo; Rachmawati, Diah; Putra, Eka Tarwaca Susila; Utomo, Condro; Liwang, Tony; Daryono, Budi Setiadi
Indonesian Journal of Biotechnology Vol 30, No 4 (2025)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijbiotech.109941

Abstract

Phosphorus (P) is an essential element for oil palm growth and development. Acid phosphatase (Apase) and Pti‐interacting serine/threonine kinase are two enzymes which enzymes confirmed to be related to P‐uptake in oil palm, therefore their activities in oil palm treated with P‐limitation need to be quantified. Acid phosphatase is believed to be induced by P‐deprivation. Conversely, the Pto‐interaction (Pti) serine/threonine kinase activity is associated with abiotic stress. The aim of this study was to quantify of activities of two selected enzymes and phytohormone content in oil palm‐clones in the P‐limitation condition. Two oil palms genotypes were treated with three P dosages i.e. 0% (v/v), 4.67% (v/v), and 14.02% (v/v) represented as starvation, deficiency, and optimum condition, respectively. The activity of these two enzymes was quantified in mitochondria and cytoplasm using spectrophotometry and modified dot‐blot methods, while abscisic acid, indole acetic acid and gibberellic acid content was quantified using ultra performance liquid chromatography (UPLC). The result showed that the Apase activity in P‐optimum was higher than starvation and deficiency in leaf and root tissues in both genotypes, whereas Pti serine/threonine kinase activity was higher in prolific than non‐prolific genotypes in P‐deficient dosage. Furthermore, abscisic acid content was higher in prolific than non‐prolific genotypes in starvation and deficient, whereas other hormone contents were similar. Association study showed that prolific was separated with non‐prolific ones at different doses of P. Finally, the prolific genotype is more adaptable with P deficiency.
Evaluation of synthetic‐gene recombinant LipL32 antigen for IgM ELISA detection of Leptospira infection Widiastuti, Dyah; Paramita, Dewi Kartikawati; Murwanti, Retno; Kusrini, Ina; Wijayanti, Nastiti; Haryanto, Aris
Indonesian Journal of Biotechnology Vol 30, No 4 (2025)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijbiotech.111102

Abstract

Leptospirosis presents with nonspecific clinical features and requires time‐consuming laboratory tests for gold standard diagnosis. This study aims to design and characterize the recombinant LipL32 from synthetic gene and assess its performance as an antigen for detecting leptospirosis. The antigen was developed by cloning the LipL32 gene conserved portion of Leptospira interrogans serovar Icterohaemorrhagiae strain Langkawi. The immunoinformatic was used to characterize the developed rLipL32. Western blot results using anti‐histidine revealed a band of rLipL32 protein at ~40 kDa. Subsequently, it was used to examine the IgM antibody on human sera by using ELISA. The IgM‐LipL32 ELISA was evaluated using 67‐positive and 25‐negative sera and compared with a commercial ELISA. With a cut‐off value of 0.8, it showed 85.7% sensitivity, 83.3% specificity, a 48% positive prediction value (PPV), and 97% negative prediction value (NPV), indicating modest performance compared to existing commercial kits. The rLipL32 is a potential antigen for detecting IgM using ELISA; however, for use in low incidence areas, a confirmation test is crucial.
Orchid‐associated endophytic Bacillus mediates Fusarium suppression and promotes in vitro regeneration of banana plantlets via culture supernatant Sasongko, Aries Bagus; Purwestri, Yekti Asih; Daryono, Budi Setiadi; Subandiyah, Siti
Indonesian Journal of Biotechnology Vol 30, No 4 (2025)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijbiotech.111727

Abstract

The application of Fusarium‐antagonistic endophytic bacteria with plant growth‐promoting traits offers an effective method to enhance the success of banana plantlet tissue culture while combating Fusarium wilt disease caused by Fusarium oxysporum f.sp. cubense Tropical Race 4 (FocTR4) (VCG 01213). This study evaluates the endophytic bacterium AP3311, isolated from healthy banana roots in direct association with orchid roots. AP3311 exhibited strong antagonism toward FocTR4, hyphal colonization ability, and multiple growth‐promoting activities, including phosphate solubilization, nitrogen fixation and auxin production. 16S rRNA gene sequencing identified that AP3311 belongs to the genus Bacillus, while metabarcoding analysis revealed that Bacillus species dominate the root microbiomes of both bananas and orchids. The bacterial supernatants stimulated root development and leaf growth in vitro. Metabolomic profiling indicated that antimicrobial compounds, together with plant growth regulators, promoted both root and shoot growth. Overall, the research demonstrates that Bacillus sp. AP3311 and its supernatants are valuable components in banana tissue culture, providing the dual benefits of plant growth promotion and effective disease control.
Decolorization of naphthol batik effluents using immobilized enzyme of Aspergillus sp. GPN and zeolite‐activated carbon in a wastewater treatment plant (WWTP) Dewi, Ratna Stia; Ramadani, Putri; Aziz, Saefuddin
Indonesian Journal of Biotechnology Vol 31, No 1 (2026)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijbiotech.82406

Abstract

Industrial batik wastewater containing naphthol dyes is persistent, dark in color, and contains complex organic compounds that have the potential to pollute aquatic environments. Conventional physicochemical treatment methods are often ineffective in degrading resistant aromatic compounds and involve high operational costs, thus necessitating alternative approaches that are more environmentally friendly and sustainable. This study evaluates the effectiveness of extracellular enzymes from Aspergillus sp. immobilized in a chitosan matrix, combined with a zeolite‐activated carbon adsorbent medium, in the decolorization of naphthol dye wastewater in a treatment system using a wastewater treatment plant. The enzymes were obtained from liquid cultures and subsequently immobilized using an encapsulation method in chitosan beads. The treatment process was conducted over 72 hours of incubation, with observations of the percentage of dye decolorization. The results show that the combination of immobilized enzymes and zeolite‐activated carbon provides a significant increase in decolorization efficiency compared to single treatments. The highest decolorization was obtained in black‐blue naphthol waste, with an efficiency of 86.94% after 72 hours of incubation. These findings indicate that a combination system of immobilized biocatalysts and adsorbents has the potential to become a more stable and effective alternative technology for batik waste treatment. The approach opens up opportunities for the development of more applicable and sustainable enzyme‐based waste treatment systems on an industrial scale.
The role of astaxanthin‐Cu2+ in stabilizing glycated human serum albumin for type 2 diabetes mellitus management: a computational approach Abiyyu, Naufal; Fitrianita, Alfia; Artika, I Made; Siregar, Josephine Elizabeth; Wibowo, Syahputra; Wardhani, Bantari Wisynu Kusuma; Budi, Canggih Setya; Nuringtyas, Tri Rini
Indonesian Journal of Biotechnology Vol 31, No 1 (2026)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijbiotech.109830

Abstract

Type 2 diabetes mellitus (T2DM) leads to the non‐enzymatic glycation of proteins, resulting in the formation of advanced glycation end products (AGEs), which contribute to diabetic complications. Human serum albumin (HSA), a major plasma protein, undergoes structural alterations upon glycation (gHSA), reducing its stability and biological functions. Astaxanthin (ASX), a potent antioxidant, is limited by its instability and moderate binding affinity. In this study, we explore the use of copper (Cu2+) to form a stable ASX‐Cu2+ complex, enhancing the antioxidant properties of ASX and improving its interaction with HSA and gHSA. Utilizing computational approaches such as molecular docking, molecular dynamics (MD) simulations, and free energy landscape (FEL) mapping, we analyze the stability and conformational changes of HSA and gHSA upon binding with ASX and ASX‐Cu2+. The residue interaction network (RIN) analysis reveals that ASX‐Cu2+ complexes create a more robust and interconnected network of non‐covalent interactions, particularly enhancing hydrogen bonding, π‐stacking, and ionic interactions. The ASX‐Cu2+ complex at a 1:2 molar ratio significantly improved the binding affinity and structural stability of both native and glycated HSA, reducing protein fluctuations and promoting a more compact conformation. These findings suggest that ASX‐Cu2+ complexes offer therapeutic potential for stabilizing albumin under glycation‐induced stress, with implications for managing oxidative stress and diabetes‐related complications.
Chitosan nanoparticle‐mediated delivery of anti‐miR‐203a‐3p for 4T1 triple‐negative breast cancer Temartenan, Jecklyn Shindy; Fiqri, Hairil; Satriyo, Pamungkas Bagus; Haryana, Sofia Mubarika
Indonesian Journal of Biotechnology Vol 31, No 1 (2026)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijbiotech.110535

Abstract

Anti‐miR molecules can suppress specific microRNA (miRNA) functions within critical signaling pathways. Chitosan acts as a delivery system for miRNAs; therefore, encapsulating miR‐203a‐3p is essential for targeted delivery and biological activity. This study investigates the impact of chitosan‐encapsulated anti‐miR‐203a‐3p nanoparticles (CS‐NPs) on the viability, proliferation, and migration of triple‐negative breast cancer (TNBC) 4T1 cells. The nanoparticles were synthe‐ sized using the ionic gelation method in a 5:1 ratio of chitosan to anti‐miR‐203a‐3p, incorporating sodium tripolyphosphate (STPP) as a crosslinker. Characterization was conducted using gel electrophoresis and particle size analysis. Cytotoxicity and cell viability were assessed through the MTT assay, while colony formation and wound healing assays evaluated cell proliferation and migration. The nanoparticles demonstrated an encapsulation efficiency of 89.47% and showed significant inhibitory effects on 4T1 cell proliferation and migration. The MTT results indicate an IC50 value of 2.454 µM, while colony formation analysis revealed that both ½ and IC50 doses significantly reduced colony numbers compared to the control. Similarly, wound healing assays showed notable inhibition of cell migration at ¼, ½, and IC50 concentrations. These findings suggest that anti‐miR‐203a‐3p‐loaded CS‐NPs may offer a promising therapeutic approach to managing aggressive breast cancer subtypes, particularly TNBC.
Integrated metabolite profiling and in silico screening reveal the cholesterol‐modulating potential of tauco, an Indonesian fermented soybean paste Nabillah, Salfa Athallah Agtari; Wati, Roidah Cahya; Nurmilah, Siti; Fitrianto, Nur; Ningsih, Dian Riana; Frediansyah, Andri
Indonesian Journal of Biotechnology Vol 31, No 1 (2026)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijbiotech.110562

Abstract

Tauco, a traditional Indonesian fermented soybean paste, is a promising yet under‐characterized source of dietary bioactives. To systematically evaluate its functional potential, we first identified ultrasonic‐assisted extraction (UAE) as the most efficient method, yielding the highest total phenolic content (28.70 mg GAE/g) and the strongest in vitro antioxidant activity (ABTS IC50 = 0.238 mg/mL). Untargeted LC–HRMS‐based metabolite profiling of this optimal extract revealed a diverse phytochemical profile rich in isoflavone aglycones, sterols, and specialized lipids. To link this chemical inventory to a specific health‐related mechanism, we performed molecular docking against HMG‐CoA reductase (HMGR), the rate‐limiting enzyme in cholesterol biosynthesis. This in silico screening prioritized the triterpenoid uvaol and the phytosterol stigmasterol as top‐binding candidates, with predicted binding affinities stronger than the reference ligand (−8.2 and −7.6 kcal/mol respectively). Overall, our integrated approach shows that UAE efficiently recovers a complex mixture of antioxidants from tauco and identifies specific metabolites with high predicted affinity for a key cardiometabolic target, providing a mechanistic hypothesis for its functional benefits and prioritizing lead compounds for future validation.
Detection of homologous plastic PET‐degrading enzyme‐encoding DNA from enriched plastic‐contaminated soil samples Wulandari, Sri Rezeki; Sabbthini, Gabriela Christy; Trinugroho, Joko Pebrianto; Nurhayati, Niknik; Ulfah, Maria; Helianti, Is
Indonesian Journal of Biotechnology Vol 31, No 1 (2026)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijbiotech.112331

Abstract

Piscinibacter sakaiensis, first isolated in Japan, is the only well‐characterized bacterium known to possess both PETase and MHETase, enabling complete polyethylene terephthalate (PET) degradation. To date, no additional habitats for the species have been reported. This study aims to identify homologous PETase and MHETase DNA from plastic‐contaminated landfill soils in Indonesia. Enrichment cultures were established from soil samples collected at Galuga (Bogor) and Cipeucang (South Tangerang). PCR amplification and sequencing revealed a full‐length MHETase homolog (G2MHETase, 1,812 bp) from Galuga, showing 99.4% and 99.3% nucleotide identity to MHETase from P. sakaiensis and Delftia sp. respectively. The deduced amino acid sequence shared 98.5% identity with both. In contrast, a partial PETase homolog (502 bp of 873 bp) was amplified from the Cipeucang sample, displaying 96 and 93% amino acid identity to PETase from P. sakaiensis and P. gummiphilus respectively. Nanophore NGS analysis of bacterial diversity indicated distinct microbial community profiles between the two sites. Rare taxa potentially associated with the detected genes included P. gummiphilus, Delftia sp., Delftia tsuruhatensis and Xenophilus aerolatus from Galuga, and Piscinibacter and Acidovorax from Cipeucang. These findings demonstrate the feasibility of detecting homologous PET degrading enzyme genes from plastic‐contaminated soils using PCR‐based approaches.
Decoding the role of tannic acid in wound healing: a dual‐action mechanism linking IL‐1β modulation and FGF‐driven tissue repair Swastini, Dewa Ayu; Nugroho, Agung Endro; Martien, Ronny; Fachiroh, Jajah; Khafi, Muhammad; Putra, Komang Dian Aditya
Indonesian Journal of Biotechnology Vol 31, No 1 (2026)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijbiotech.113925

Abstract

Tannic acid (TA) has been shown in a previous study to expedite cutaneous wound healing in rats; however, the precise mechanism by which it operates remains poorly understood. This research evaluates the effects of TA on wound healing using both in vitro and in silico methods. In vitro, its influence on the inflammatory cytokine interleukin‐1β (IL‐1β) and the growth factor fibroblast growth factor (FGF) throughout the healing process were assessed. In silico molecular docking was employed to predict direct ligand–protein interactions and to provide a mechanistic insight into whether these proteins represent primary molecular targets or downstream effects. Parameters evaluated included cell viability and proliferation, scratch assays, and the activity of pro‐inflammatory cytokines in the lipopolysaccharide (LPS)‐stimulated RAW 264.7 macrophage cell line, together with growth factors in the NIH 3T3 fibroblast cell line; all were evaluated using enzyme‐linked immunosorbent assay (ELISA). The results indicate that TA significantly facilitates wound closure by promoting NIH 3T3 fibroblast cell proliferation, enhancing FGF expression, and suppressing IL‐1ß synthesis in both in vitro and in silico approaches. These findings suggest that TA may hold considerable promise for wound‐healing management.
Recombinant expression and preliminary characterization of a synthetic L‐asparaginase from the marine bacterium Pseudoalteromonas tetraodonis GFC in Escherichia coli BL21 (DE3) Pertiwi, Wulan; Tohari, Taufik Ramdani; Suhada, Qori Atur Rodiah; Zahira, Tiara; Nugraha, Nadila Suci
Indonesian Journal of Biotechnology Vol 31, No 1 (2026)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijbiotech.114307

Abstract

The marine environment represents a promising source of diverse enzymes produced by marine microorganisms, including L‐asparaginase. This has been widely studied due to its ability to hydrolyze extracellular L‐asparagine, an amino acid required for the growth of certain cancer cells. In this study, a synthetic L‐asparaginase gene derived from the marine bacterium Pseudoalteromonas tetraodonis GFC was recombinantly expressed in Escherichia coli BL21 (DE3). The gene was cloned into the pD861‐SR expression vector and transformed into E. coli BL21 (DE3). Positive transformants were confirmed by restriction digestion using SapI and Sanger sequencing. Recombinant protein expression was induced with L‐rhamnose under the control of the rhaBAD promoter. The expressed L‐asparaginase was then purified using Ni‐Sepharose affinity chromatography followed by membrane dialysis, yielding a protein purity of 73.6%. SDS‐PAGE analysis revealed a prominent protein band at approximately 37 kDa, corresponding to the expected molecular weight of recombinant L‐asparaginase. Enzymatic activity was evaluated using the Nessler method, and the purified enzyme exhibited a specific activity of 5.863 U/mg. These results demonstrate the successful recombinant expression and preliminary functional validation of L‐ asparaginase from P. tetraodonis GFC in E. coli, providing a basis for further optimization and characterization in future studies.

Page 53 of 53 | Total Record : 530

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