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Biota
Published by Universitas Islam Negeri Raden Fatah Palembang
ISSN : 2528262X     EISSN : 24607746     DOI : https://doi.org/10.19109/Biota
Core Subject : Health, Science, Agriculture, Social,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Materials Science & Nanotechnology Medicine & Pharmacology
Jurnal biota is an open access, peer-reviewed, multidisciplinary journal dedicated to the publication of novel research in all aspects of biology, with particular attention paid to the exploration and biodiversity Wetland tropical—and especially Indonesian—biodiversity. Original research articles written in English and featuring well-designed studies with clearly analyzed and logically interpreted results are accepted, with a strong preference given to research that has the potential to make significant contributions to both the field of biology and society in general
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Pertanian dan Biologi (Lain-Lain)
Articles 160 Documents
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UJI KANDUNGAN BORAKS PADA PEMPEK LENJER YANG DIJUAL DI KELURAHAN PAHLAWAN Irham Falahudin; Elfira Rosa Pane; Niar Kurniati
Biota Vol 2 No 2 (2016): Vol 2 No 2 Agustus 2016
Publisher : Faculty of Science and Technology Universitas Islam Negeri Raden Fatah Palembang

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Abstract

Pempek is processing product of fish meat and tapioca flour. Pempek has a low shelf life because the process of making it that is boiled first, it makes pempek easy to be slimy. It is one of the reason why the Pempek seller uses dangerous preservative such as borax. The purpose of this research is to know whether Pempek Lenjer that is sold in Pahlawan Palembang Village contains borax or not,  and contribution in the form of student worksheet (LKS) and poster. This research uses organoleptic test, then color test by using tumerik paper to know the existence of  borax in Pempek Lenjer at sold in Pahlawan, Kilometer 3,5, Palembang City, South Sumatera, Indonesia. Date research on 17-24st June 2015.  In this research, organoleptic test indicates that there is no Pempek Lenjer that has a characteristic containing borax, on the colour test all sampels lenjer pempek doesn’t contain borax, because it does not change color on paper tumerik that has dripped samples, performed three, and it is done validation in the student worksheet (LKS) with 5 validators from 3 schools and the result of validation of the student worksheet (LKS) from 5 validators is valid
PENGARUH KUANTITAS GARAM TERHADAP KUALITAS BEKASAM zainal burlian; Syarifah Syarifah; Imamul Huda
Biota Vol 2 No 2 (2016): Vol 2 No 2 Agustus 2016
Publisher : Faculty of Science and Technology Universitas Islam Negeri Raden Fatah Palembang

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Abstract

Fish is one of the animal protein sources consumed by people because it is relatively easy to obtain and affordable. The abundant of the fish needs some ways to preserve it, one of them is by making bekasam. Bekasam is fish fermented product which is procesed with the addition of rice and salt. One of the function of the salt is to select microorganisms that produce proteolytic enzymes.The purpose ofthis study was to know influance of different salt concentrations to ward the quality of fish bekasam.The research was conducted in August 2015 in Chemistry Laboratory of Biology Education Study Program, Teaching Training and Tarbiyah Faculty UIN Raden Fatah Palembang. The research method was an experimental laboratoryusing a CRD with 4 treatments and 3 replication. The treatments were salt concentrationK0 (10% b/v), K1 (20% b/v), K2 (30% b/v) and K3 (40% b/v). The test parameters observed were theacidity and hedonic test (color, smell and taste). The parametric data for the acidity was analyzed by ANOVA test, while nonparametric data(hedonic) used the organoleptic test. The results showed that based on ANOVA, the different salt concentration gave a very significant different influance (P=0,01) toward the acidity value, that Fcount> Ftable is 26,16 > 7,59. Based on research result, the acidity of each K0, K1, K2and K3 was 1,13 N, 1,00 N, 1,02 Nand  0,42N.Treatment of 10% salt concentration resulted the highest  acidity 1,13 N.While from hedonic test showed that bekasam with 20%salt concentration was the most preferred
PENGARUH KADAR THIAMINE (VITAMIN B1) TERHADAP PERTUMBUHAN JAMUR TIRAM PUTIH (Pleurotus ostreatus) Munir Munir; Fitratul aini; Siti Jariah
Biota Vol 2 No 2 (2016): Vol 2 No 2 Agustus 2016
Publisher : Faculty of Science and Technology Universitas Islam Negeri Raden Fatah Palembang

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Abstract

Indonesia is one of the centers of biodiversity in particular areas of food, which one of them is from a species of white oyster mushroom (Pleurotus ostreatus). To improve the effectiveness and efficiency of production time associated with fulfilling the request of the oyster mushroom (Pleurotus ostreatus) is high in the market, the utilization of thiamine (vitamin B1) need to be investigated. This study aims to determine the levels of thiamine (vitamin B1), which is best used in the growth of oyster mushroom (Pleurotus ostreatus). This study used quantitative experiments and research design complete randomized design with four treatments and six times as many repetitions as well as research along with the observations made on 21 June to 11 July 2015 (in 20 days) in the House of White Oyster Mushroom Production Nurseries, Ogan Komering Ulu Timur (OKUT). As for the levels of thiamine (vitamin B1) used in this study use the unit ppm (parts per million), namely: B0 (control), B1 (0.1 ppm), B2 (0.2 ppm), B3 (0.3 ppm). Parameters measured were the growth of oyster mushroom (Pleurotus ostreatus) which include emerging buds time (day), the time of harvest (days), weight (grams), wide hood (cm), and stem length (cm). Conclusions from the study and based on data analysis (ANOVA) and F test showed that administration of thiamine (vitamin B1) does not provide an effective influence on the growth of oyster mushroom (Pleurotus ostreatus). 
PENGARUH EKSTRAK BATANG BROTOWALI (Tinospora crispa) TERHADAP KEMATIAN LARVA NYAMUK Aedes aegypti Agustiani Dumeva; Syarifah Syarifah; Syahidah Fitriah
Biota Vol 2 No 2 (2016): Vol 2 No 2 Agustus 2016
Publisher : Faculty of Science and Technology Universitas Islam Negeri Raden Fatah Palembang

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Abstract

Aedes aegypti mosquito, which acts as a vector in the spread of dengue disease. One of the effort to reduce thespread of dengue disease is using larvicides. Brotowali thought to have an effect as larvicides against Aedesaegypti larvae because they have the alkaloid compounds and tinokrisposid that can cause death in larvae. Thisstudy aims to determine the effect extract brotowali (Tinospora crispa) against Aedes aegypti larvae of death soas to provide information to the public that can be used as larvicidal brotowali. This research is an experimentwith a completely randomized design (CRD) with 4 treatments and 6 replicates. The samples used were thirdinstar larvae of Aedes aegypti with the treatment A0 (abate 500 ppm), A1 (500 ppm), A2 (750 ppm), and A3(1000 ppm). Observations dead larvae were carried out for 24 hours and 48 hours. The results showed thatwithin 24 hours at a concentration of 500 ppm on average larvae death reached 32.5%, the concentration of 750ppm 55.8%, a concentration of 1000 ppm to 70%, whereas within 48 hours at a concentration of 500 ppm onaverage larvae death reached 75%, 90.8% concentration of 750 ppm, a concentration of 1000 ppm to 100%.Based on analysis of variance (Ansira) showed that the extract brotowali (Tinospora crispa) gives a significantinfluence (p > 0.01) in the death of larvae of Aedes aegyti. Effective concentration to kill larvae of Aedes aegytinamely the concentration of 750 ppm.
SKRINING BAKTERI SELULOLITIK ASAL TANAH KEBUN PISANG meli Astriani
Biota Vol 3 No 1 (2017): Vol 3 No 1 Januari 2017
Publisher : Faculty of Science and Technology Universitas Islam Negeri Raden Fatah Palembang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19109/Biota.v3i1.871

Abstract

Cellulose is the most abundant biomass in nature. Utilization of organic wastes can be improved by changing the cellulose from wastes into products which have the economic value with the help of cellulolytic bacteria. Cellulolytic bacteria are bacteria that have the ability to produce cellulase. Cellulase can degrade cellulose as the substrate. The aim of this study were to obtain cellulolytic bacteria, and to provide an information about the test of cellulolytic bacterial activity. Stages of this study are as follows:  isolation and purification, morphological and physiological characterization of cellulolytic bacteria, tests enzyme activity. Results showed that there were two types of cellulolitic bacteria which had been obtained, namely isolate A1 and A2 that were able to grow on CMC media and each isolate has the ability to form a clear zone of 1.3 and 1.25 mm, respectively. Isolate A1 belonged to genus Pseudomonas with enzyme activity of 1.248 unit/ml.
PEMANFAATAN KULIT BUAH JERUK NIPIS (Citrus aurantifolia) SEBAGAI LARVASIDA Aedes aegypti INSTAR III evy Ratnasari Ekawati
Biota Vol 3 No 1 (2017): Vol 3 No 1 Januari 2017
Publisher : Faculty of Science and Technology Universitas Islam Negeri Raden Fatah Palembang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19109/Biota.v3i1.926

Abstract

DHF (Dengue) disease is transmitted through mosquito vectorAedes aegypti.One way to do control the Dengue vector is to use insecticide made from natural materials and is environmentally friendly with lemon peel extract (Citrusaurantifolia). This study aims to determine the effectiveness of extracts of lemon peel (Citrus aurantifolia) in killing the third instar larvae Aedesaegypti with various concentrations and time variations. This were an experimental laboratory researchs using various concentrations of extract limes peel (Citrusaurantifolia) 1%, 2%, 3%, 4%, 5%, 6%, and 7% in which there 25 larvae Aedes aegypti and using a variation of 30, 60, 90, 120, 180, 240, 300, and 1440 minutes after contact with the extracts limes peel (Citrusaurantifolia). Effective concentration to kill larvae of Aedes aegypti by 50% (IC50) was the concentration of 3.419%.
ANALISA KADAR PROTEIN CRUDE ENZIM SELULASE DARI KAPANG Rhizopuz Sp PADA SUBSTRAT AMPAS TEBU HASIL ISOLASI DARI KEBUN CENGKEH, KARE, MADIUN Pujiati pujiati
Biota Vol 3 No 1 (2017): Vol 3 No 1 Januari 2017
Publisher : Faculty of Science and Technology Universitas Islam Negeri Raden Fatah Palembang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19109/Biota.v3i1.930

Abstract

Kapang Rhizopus sp merupakan salah satu mikroorganisme yang memiliki kemampuan tinggi untuk menghasilkan enzim selulase.Enzim selulase merupakan enzim yang dapat menghidrolisis selulosa. Hidrolisis meliputi proses pemecahan polisakarida di dalam biomassa lignoselulosa, yaitu: selulosa dan hemiselulosa menjadi monomer gula penyususnnya. Penelitian ini bertujuan untuk mengetahui produksi dan aktivitas enzim selulase terhadap aktivitas crude enzim selulase dari kapang Rhizopus sp dengan subsrtat ampas tebu (bagase). Metode penelitian menggunakan kuantitatif eksperimen dengan pola rancangan acak lengkap (RAL) dua faktorial. Perlakuan penelitian meliputi perbedan inokulum (K) yaitu 5% (K1), 15% (K2), 25% (K3) dan lama fermentasi (T) yaitu 3hari (T1), 6hari (T2), 9hari (T3), dan 12hari (T4). Data yang diambil dari perlakuan tersebut adalah kadar protein dengan metode brownstead lowry. Analisis data menggunakan variansi anava dua jalur dengan taraf signifikansi 5% setelah itu dilanjutkan dengan uji Beda Nyata Terkecil (BNT ). Hasil penelitian menunjukkan bahwa: Fhit > Ftab sehingga ada pengaruh antara konsentrasi inokulum dan lama fermentasi terhadap aktivitas crude enzim selulase dari kapang Rhizopus sp, Perlakuan perbedaan konsentrasi dan lama fermentasi mendapatkan kadar protein tertinggi 0,715 dengan konsentrasi 25%  dan lama fementasi 25%
SKRINING AKTIVITAS ANTIBAKTERI DAUN KELOR (Moringa oleifera), DAUN BIDARA LAUT (Strychnos ligustrina Blume), DAN AMOXICILIN TERHADAP BAKTERI PATOGEN Staphylococcus aureus Edi Suriaman
Biota Vol 3 No 1 (2017): Vol 3 No 1 Januari 2017
Publisher : Faculty of Science and Technology Universitas Islam Negeri Raden Fatah Palembang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19109/Biota.v3i1.952

Abstract

Exploration of a variety of plants as a source of antibacterial has been done, but it is not all plants effective to inhibit the growth of bacteria. This study aims to determine the difference between the ability of the antibacterial activity of plant leaves Moringa oleifera and Strychnos ligustrina Blume against Staphylococcus aureus bacteria growth. This research is an experiment with a completely randomized factorial design (CRD) 3x4 with four replicates. The materials use is ethanol extract of the leaves of Moringa leaf extract, S. ligustrina, and amoxicillin. Testing the antibacterial activity performed on Mueller-Hinton agar media with the diffusion method. The analysis and interpretation of results were done by ANOVA 95% (α: 0.05). Susceptibility bacteria to antibiotics, and plant extracts assessed according to standard NCCLS (National Committee for Clinical Laboratory Standards). The antibacterial agent (ethanol extract of Moringa leaf, ethanol extract of S. ligustrina leaf, and amoxicillin) and concentrations influence significantly inhibit the growth of S. aureus bacteria. S. aureus bacteria are sensitive only at 100% concentration with inhibition zone diameter 21.5 ± 5:35 mm, when given the leaf extract of S. ligustrina, and at a concentration of 75% with a diameter of 14.3 ± 1.32 mm is intermediate. The bacteria behave-resistant to the compound of M. oleifera leaf extract and amoxicillin at all concentrations. Diameter of zones of inhibition amoxicillin equal 9.99 ± 2.02 mm with 100% concentration treatment.  While the treatment of M. oleifera leaf extract, at a concentration of 75% and 100%, resulting in the largest inhibition zone each is 3.9 ± 0.64 and 3.82±0.57 mm.
PENGEMBANGAN PETUNJUK PRAKTIKUM MIKROBIOLOGI PROGRAM STUDI PENDIDIKAN BIOLOGI Trio Ageng Prayitno
Biota Vol 3 No 1 (2017): Vol 3 No 1 Januari 2017
Publisher : Faculty of Science and Technology Universitas Islam Negeri Raden Fatah Palembang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19109/Biota.v3i1.1041

Abstract

The learning process of mikrobiology requires a microbiological lab instruction. This is needed in practical activities in the Laboratory to provide mastery minds on and hands on the students. This research aims to produce a valid microbiological lab instructions. This research is a research & development. That model of development used 4D Thiagarajan. 4D model include define stage, design stage, develop stage, and desseminate stage. Limitation of the research is only at develop stage. The instrument of research used questionnaire validation and responses. Data of the research was percentage of validity. The results showed that a microbiological lab instruction was valid. Advice, a microbiological lab instruction can be used in the learning process of microbiology.
ANALISIS ZAT PEWARNA RHODAMIN B PADA SAUS CABAI YANG BEREDAR DI KAMPUS UNIVERSITAS ISLAM NEGERI RADEN FATAH PALEMBANG Debby Chrislia
Biota Vol 3 No 1 (2017): Vol 3 No 1 Januari 2017
Publisher : Faculty of Science and Technology Universitas Islam Negeri Raden Fatah Palembang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19109/Biota.v3i1.1090

Abstract

Chili sauce is one of the most popular food product because it was used as food complement. Generally in processing manufacture chili sauce added coloring substance, so product colour interesting. Nowadays more and more food manufacturers use dye that have been banned use. Rhodamine B dye in the form of a crystalline powder green or reddish purple, odorless and dissolves easily in bright red solution berfluoresan as textile dyes. Rhodamin b is still no food products were found to countain rhodamine B dye such as crackers, sauces, ice and other pastries. Rhodamin B is a synthetic dye that was banned for use in foods and is certified as a hazardous material according to Minister of Healthy of Indonesian Republic No. 722/Menkes/Per/IX/1998. Rhodamin B is carcinogenic, in the long term use can cause liver damage, kidney inflammation, and cancer. The kind of research this used a quantitative approach with the experimental methods laboratory. The purpose of this study was to determine whether there rhodamin B in the test samples using thin layer chromatography (TLC). The result shows that 7 examined samples doesn’t contain rhodamin B and and shows f count smaller than f table (F count< F table) (3,61< 3,71). Synthetic dyes are contained in most of the analyzed samples are dyes that allow its use for food by the Regulation of the Minister of Health of Indonesia such as ponceau 4R and amarant and Contribution of research given by researchers of worksheets students and implementation plan learning

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