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Jurnal Ilmu Farmasi dan Farmasi Klinik (Journal of Pharmaceutical Science and Clinical Pharmacy)

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Published by Universitas Wahid Hasyim Semarang

ISSN : 16937899 EISSN : 27163814 DOI : 10.31942

Core Subject : Health,

Medicine & Pharmacology

Selamat datang di situs e-Publikasi Ilmiah Fakultas Farmasi Unwahas (Universitas Wahid Hasyim) Semarang. Situs ini berisi kumpulan publikasi ilmiah yang diterbitkan oleh Fakultas Farmasi Unwahas. Publikasi berasal dari jurnal-jurnal serta hasil prosiding seminar yang dilaksanakan oleh Fakultas Farmasi Unwahas.

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Articles 349 Documents

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EVALUASI KUALITAS PENGGUNAAN ANTIBIOTIKA PADA PASIEN PASCA BEDAH DENGAN METODE GYSSENS DI RSUD BDH SURABAYA PERIODE 2016 Hajar Sugihantoro; Abdul Hakim; Nur Miya Zakiya
Jurnal Ilmu Farmasi dan Farmasi Klinik Vol 17, No 01 (2020): Jurnal Ilmu Farmasi & farmasi Klinik Vol 17 No. 01, Juni 2020
Publisher : Universitas Wahid Hasyim Semarang

ABSTRACT The rational use of antibiotics is prescribing antibiotics, dosage, duration, safe and affordable interval of medicine use by the patient. The purpose of this research is to know the quality of antibiotics use to the post-surgery patients in BDH Surabaya for 2016. This is a cross-sectional design, collecting the data from the medical records of post-surgery patients who received antibiotics in BDH Surabaya during 2016. The sample was taken by proportional stratified sampling. The quality of antibiotics use was analyzed by using Gyssens. The result showed that from reviews those 97 medical records, the total of the rational use of antibiotics in BDH Public Hospital Surabaya were 59.7% (category 0) and 40.3% included to the category II-IV. The use of antibiotics with incorrect dosages (category IIA) 4.8%, inappropriate administration intervals (category IIB) 7.7%, too long use (category IIIA) 16.3%, too short use (category IIIB) 1.9%, there was more effective antibiotics (category IVA) 2.9%, and there was other cheaper antibiotics (category IVC) 6.7%. Based on the quality of the use of antibiotics in post-surgical patients in BDH Hospitals at 2016 period, that appropriate Gyssens category rational antibiotic use was 59.7% and the results are not rational as much as 40.3%. Keywords: antibiotic, rational, quality, Gyssens

VALIDASI METODE ANALISIS KLORFENIRAMIN MALEAT DAN GUAIFENESIN MENGGUNAKAN KROMATOGRAFI CAIR KINERJA TINGGI SERTA APLIKASINYA DALAM SEDIAAN SIRUP Aqnes Budiarti; Ira Afifah
Jurnal Ilmu Farmasi dan Farmasi Klinik Jurnal Ilmu Farmasi & Farmasi Klinik Vol 14 No 1 juni 2017
Publisher : Universitas Wahid Hasyim Semarang

INTISARI Kombinasi klorfeniramin maleat (CTM) dan guaifenesin (GG) digunakan untuk meringankan gejala batuk dan pilek. Kadar CTM dan GG dalam sirup sangat kecil sehingga penetapan kadarnya memerlukan metode yang valid. Tujuan penelitian ini adalah memvalidasi metode penetapan kadar CTM dan GG dan mengaplikasikannya dalam beberapa sediaan sirup di pasaran. Penetapankadar CTM dan GG menggunakan KCKTJascoLc-Net II/ADC dengandetektor UV-Visible. Fase diam berupa C18Li Chospher dan fase gerak adalah campuran asetonitril:metanol:air (15:10:75 v/v), waktu alir 1 mL/menit pada panjang gelombang 270 nm. Validasi metode meliputi akurasi, presisi, selektivitas, linieritas dan sensitivitas. Metode analisis diaplikasikan terhadap 3 merk sirup dari pabrik yang berbeda. Hasil penelitian menunjukkan bahwa validasi metode penetapan kadar CTM dan GG memenuhi persyaratan yaitu: uji presisi menghasilkan %RSD untuk CTM 0,18% dan GG 0,17%;ujiakurasi menghasilkan perolehan kembali yang memenuhi persyaratan baikuntuk CTM maupun GG pada sirup ketigamerksirup. Selektivitasnya baik; linieritas memenuhi syarat; LOD CTM dan GG masing masing sebesar 2,40 µg/mL dan 3,07 µg/mL; LOQ CTM dan GG masing-masing sebesar 8,00 µg/mL dan 10,24 µg/mL. Kadar rata-rata CTM dan GG pada ketiga sirup memenuhi persyaratan kadar yang ditetapkan oleh Farmakope Indonesia Edisi V. Kata kunci : validasi, klorfeniramin maleat, guaifenesin, KCKT ABSTRACT The combination of CTM and GG is often used as an active substances to relieve symptoms of cough and cold. The concentration of CTM and GG in syrup are very low, so that it need validated determination method to ensure therapeutic dose. The purposes of this study were to validate determination method of CTM and GG and to apply the method to some syrups. Determination of CTM and GG in syrup using HPLC JascoLc-Net II/ADC equipped with a UV-Visible detector at a wavelength of 270 nm. The stationary phase used was C18Li Chosphera nd the mobile phase usedwas a mixture of acetonitrile:methanol:water (15:10:75 v/v) with a flow rate of 1 mL/minutes.Validation test was conducted on precision test, accuracy, selectivity, linearity and sensitivity. The method of analysis was applied to syrups fromthree different factories. The result of this research showed that validation tests of CTM and GG met the requirements, were: % RSD of precision test for CTM was 0.18% and for GG was 0.17%; accuracy test resulted good recovery for CTM and for GG in syrups. Good selectivity; linearity with a correlation coefficient 0.999; LOD for CTM and GG were 2.40 and 3.07 µg/mL; LOQ for CTM and GG were 8.00µg/mL and 10.24 µg/mL. The level of CTM and GG in syrups were compliance with the requirements by the Indonesian Pharmacopeia Edition V. Keywords: validation, chlorfeniramin maleat, guaifenesin, HPLC

UJI SITOTOKSIK DENGAN METODE BRINE SHRIMP LETHALITY TEST DAN PROFIL METABOLIT SEKUNDER DARI EKSTRAK BIJI BUAH BERENUK (Crescentia cujete Linn) Afdhil Arel
Jurnal Ilmu Farmasi dan Farmasi Klinik Jurnal Ilmu Farmasi & Farmasi Klinik Vol 15 No 2 Desember 2018
Publisher : Universitas Wahid Hasyim Semarang

ABSTRACTThe purpose of this research is to find out the profile of secondary metabolite contained in the extract of seeds (Crescentia cujete Linn.) And cytotoxic test against shrimp larvae Artemia salina Leach by BSLT method. Phytochemical tests showed that the extract be expected contained the terpenoid, alkaloid, phenolic, secondary metabolite compounds. The results of qualitative analysis with positive TLC contains phenolics. The result of identification with Spectrophotometer UV-visfor determination of α max from berenuk seeds extract consisted of 3 peaks with α max 326 nm, 258 nm, 222 nm be expected terpenoid compound, alkaloid, and phenolic. The results of toxicity test using shrimp larvae (Artemia salina leach) were divided into 4 groups: 1 control group and 3 groups of concentration series. The concentration of test solution used was 1,000, 100 and 10 μg / ml. The shrimp larvae mortality was observed after 24 hours of treatment. Based on the analysis with probit method, LC50 value of seed extract berenuk to shrimp larvae Artemia salina Leach is equal to 82,30 μg/ml. This shows that the extract of berenuk berries is toxic to shrimp larvae Artemia salina Leach, presence to terpenoid content, alkaloids and phenolics in the extract of seeds berenuk.Keywords : Secondary metabolite; Cytotoxic; BSLT.

STANDARISASI NON SPESIFIK EKSTRAK ETANOL DAUN KECAPI (Sandoricum koetjape Merr.) DAN EKSTRAK ETANOL DAUN KELUWIH (Artocarpus communis) Maria Ulfah; Dhia Salsabilla; Evi Sukawati
Jurnal Ilmu Farmasi dan Farmasi Klinik Vol 16, No 02 (2019): Jurnal Ilmu Farmasi & farmasi Klinik Vol 16 No 02 Desember 2019
Publisher : Universitas Wahid Hasyim Semarang

ABSTRACT The chemicals contained in an extract must be maintained its quality. The aim of this study is to prove both that the ethanol extract of kecapi (Sandoricum koetjape, Merr.) and keluwih (Artocarpus communis) leaves meet the non-specific parameters on the standardization of the extract. The ethanol extract of kecapi and keluwih leaves were extracted using the ultrasonic method. The extract was tested with non-specific parameters including water content, total ash content and metal contamination level. The test results are compared with standard parameter references. Ethanol extract of kecapi leaf have water content of 27.75% ± 1.708, total ash content of 4.856% ± 0.040 and levels of As metal contamination < 0.030 mg/Kg, Hg < 0.002 mg/Kg, Pb < 0.400 mg/Kg, while ethanol extract of keluwih leaf have water content of 23.206 ± 0.091%, total ash content of 9,678 ± 0.306% and levels of heavy metal contamination of As < 0.03 mg/Kg, Hg < 0.006 mg/Kg, Pb < 0.02 mg/Kg. The both extract meet the standard parameters of water content, total ash content and levels heavy metal contamination. Key words: kecapi leaves (Sandoricum koetjape, Merr.), keluwih leaves (Artocarpus communis), non-specific standardization

PERBEDAAN KADAR FLAVONOID TOTAL EKSTRAK DAUN SALAM (Syzygium polyanthum) DENGAN METODE REFLUKS DARI BEBERAPA JENIS PELARUT DAN AKTIVITAS ANTIBAKTERI Yuliana Dewi Hastuti; Dewi Andini Kunti Mulangsri
Jurnal Ilmu Farmasi dan Farmasi Klinik Vol 18, No 2 (2021): Jurnal Ilmu Farmasi & Farmasi Klinik
Publisher : Universitas Wahid Hasyim Semarang

Bay leaves that it contains flavonoid compounds can inhibit the growth of Escherichia coli bacteria causes diarrhea. Hot extraction method can extract more compounds in the plants, particularly are heat stable. This study aimed to determine the different of total flavonoid content and antibacterial activity against Escherichia coli from bay leaves extracts that extracted with some solvent by reflux. The bay leaves extract was extracted with 70% ethanol, 96% ethanol, and methanol solvent. The spectrophotometric method was used to determine of total flavonoid contents at λmax 431,5 nm and operating time 30 minutes with AlCl₃ reagent. The disc diffusion method was used antibacterial activity test with chloramphenicol as a positive control and dimethyl sulfoxide as negative control. Analysis data of total flavonoid content with One Way Anova statistics, meanwhile the zone of inhibition from antibacterial activity test analysed by Two Way Anova continued with Tukey. The total flavonoid content of bay leaves extract (ethanol 70%, ethanol 96%, and methanol) were 6,089; 5,028; 4,052 mgQE/gram extract. There was significant difference of total flavonoid content between variation solvent and the most highest at 70% ethanol. Different extraction solvent and concentration serial of bay leaves extract showed significant difference and the most highest zone of inhibition at extract with 96% ethanol solvent.

EKSTRAK DAUN KELOR (Moringa oleifera Lam.) SEBAGAI PELURUH KALSIUM BATU GINJAL SECARA IN VITRO Yance Anas; Ali Imron; Sekar Indah Ningtyas
Jurnal Ilmu Farmasi dan Farmasi Klinik Jurnal Ilmu Farmasi & Farmasi Klinik Vol 13 No 2 Desember 2016
Publisher : Universitas Wahid Hasyim Semarang

INTISARI Senyawa flavonoid dalam daun kelor diduga mampu meningkatkan kelarutan kalsium batu ginjal. Penelitian ini bertujuan untuk membuktikan kemampuan ekstrak metanol (EMDK) dan ekstrak etanol daun kelor (EEDK) dalam meningkatkan kelarutan kalsium batu ginjal secara in vitro. Daun kelor diekstrak dengan cara maserasi menggunakan cairan penyari metanol dan etanol 70%. Kandungan senyawa aktif golongan flavonoid diidentifikasi secara kualitatif dengan KLT. Serbuk batu ginjal direndam dalam seri konsentrasi EMDK dan EEDK (2%, 4%, 6%, 8%, dan 10%) dan diinkubasi selama 6 jam pada suhu 37 0C. Kadar kalsium terlarut dianalisis dengan SSA pada panjang gelombang 422,7 nm. Hasil penelitian membuktikan bahwa EMDK (2-10)% dan EEDK (4-10)% mampu meningkatkan kelarutan kalsium batu ginjal secara in vitro (p≤0,05). Kelarutan kalsium batu ginjal dalam EMDK dan EEDK mengikuti pola tergantung konsentrasi. Kadar kalsium batu ginjal terlarut dalam EMDK dan EEDK berturut-turut berkisar antara (86,27-185,87) ppm dan (95,31-177,29) ppm. Hasil analisis dengan KLT membuktikan bahwa senyawa aktif golongan flavonoid berhasil terdentifikasi dalam EMDK dan EEDK. Kata Kunci: Batu ginjal kalsium, daun kelor (Moringa oleifera Lam.), flavonoid, maserasi ABSTRACT Flavonoids in kelor leaves (Moringa oleifera Lam.) suspected had an important role on dissolving calcium kidney stones. The purpose of this research is to reveal the effect of kelor leave's methanol extract (KLME) and ethanol extract (KLEE) on the in vitro solubility of calcium kidney stones. The kelor leave's simplisia extracted with maceration method. Methanol and ethanol 70% were used as solvent. An active flavonoid compound in KLME and KLEE has identified with TLC method. Kidney stone's powder soaked in KLME and KLEE series concentration (2%, 4%, 6%, 8% and 10%) and incubated for six hours at 37 °C. The dissolved calcium levels from kidney stones in KLME and KLEE were analyzed with AAS at a wavelength of 422.7 nm. The result showed that the KLME (2-10) % and KLEE (4-10%) can enhance the dissolved calcium levels from kidney stones in vitro (p<0.05). The dissolved kidney stone's calcium in KLME and KLEE deppend on it’s concentration. The levels of dissolved calcium from kidney stones in KLME dan KLEE series concentration were (86.27-185.87) ppm and (95.31–177.29) ppm repectively. The result of TLC analysis showed that the flavonoid found in KLME dan KLEE. Keywords: Calcium kidney stones, flavonoid, maceration, moringa leaves (Moringa oleifera Lam.)

FORMULASI MASKER GEL PEEL OFF DARI EKSTRAK ETANOL UMBI BAWANG DAYAK (Eleutherinebulbosa (Mill.) Urb. Eleutherine americana Merr) Dian Kartikasari; Reni Anggraini
Jurnal Ilmu Farmasi dan Farmasi Klinik Jurnal Ilmu Farmasi & Farmasi Klinik Vol 15 No 1 Juni 2018
Publisher : Universitas Wahid Hasyim Semarang

INTISARI Umbi bawang dayak memiliki kadar antioksidan yang sangat tinggi. Penelitian ini bertujuan mendapatkan formula masker gel feel off ekstrak umbi bawang dayak yang menghasilkan pembentukan film yang baik berdasarkan hasil evaluasi fisik. Masker gel peel off dibuat dalam 3 formula dengan parameter perbedaan konsentrasi PVA. Formula 1 PVA 10 %, formula 2 12%, formula 3 PVA 13%. Proses pembuatan ekstrak dengan cara maserasi, kemudian dipekatkan dengan evaporator dan dibuat sediaan masker gel peel off dengan penambahan eksipien. Hasil uji organoleptis menunjukkan parameter aroma, warna dan tekstur tidak berbeda nyata antar formula. Hasil uji fisik menunjukkan formula 3 dengan PVA 13 % menghasilkan masker gel peel off yang paling baik dibandingkan dengan formula 1 dan 2, dengan nilai viskositas 3,833 cps, pH 6, homogeny, waktu mongering < 15 menit dan uji daya sebar 5, 815 cm. Kata kunci: bawang dayak, masker gel peel off, PVA. ABSTRACT Bawang Dayak bulbs have a very high antioxidant content. This study aims to get the mask gel feel off formula the bawang Dayak bulb extract that results in good film formation based on the results of physical evaluation. The mask gel Peel off is made in 3 formulas with different parameters of PVA concentration. Formula 1 PVA 10%, formula 2 12%, formula 3 PVA 13%. The process of preparing the extract by maceration, then concentrated with evaporator and made gel mask preparation with the addition of excipients. The results of organoleptic tests showed the parameters of aroma, color and texture were not significantly different between the formulas. Physical test results showed that formula 3 with PVA 13% yielded the best peel off gel mask compared with formula 1 and 2, with viscosity value 3,833 cps, pH 6, homogeny, mongering time <15 min and spreading test 5,515 cm. Keywords: Bawang Dayak, mask gel peel off, PVA.

METODE ISOLASI YANG MUDAH DALAM ISOLASI SENYAWA β-KAROTEN DARI LABU KUNING (Cucurbita moschata Duch ex Poiret) Rollando Rollando; Muhammad Hilmi Afthoni
Jurnal Ilmu Farmasi dan Farmasi Klinik Jurnal Ilmu Farmasi & farmasi Klinik Vol 16 No 1 Juni 2019
Publisher : Universitas Wahid Hasyim Semarang

ABSTRACTPumpkin (Cucurbita moschata Duch ex Poiret), a fruit that contains several compounds, has activities to protect the human body from free radicals. Provitamin A is recognized as an antioxidant compound. Antioxidants can protect the body’s damage caused by free radicals. The purpose of this study was to isolate beta carotene compounds and test the purity of beta carotene isolates obtained using DR-UV and infrared spectrophotometry. Pumpkin is extracted using acetone: petroleum ether with a ratio (1: 1). Identification of beta carotene from extracts was carried out using TLC with the mobile phase of ethanol: acetone: n-hexane with a ratio (2: 1: 1) and scan spectrum using a UV-Vis spectrophotometer. Beta carotene was identified as the result of TLC elution on UV 254 and 366 nm which showed 2 spots. Detection of beta carotene spectra was carried out in the range of 350 to 550 nm and obtained beta carotene peaks at wavelength x nm. The extract purification process was carried out using Preparative TLC with the mobile phase of ethanol: acetone: n-hexane with a ratio (2: 1: 1) and obtained isolates cleaned first using chloroform: methanol ratio of 1: 1. The isolates were dried and tested using DR-UV and infrared spectrophotometry. Isolates from Cucurbita moschataDuch ex Poiret extract) contain beta carotene, zeaxanthin.Keywords: pumpkin; beta carotene; isolation

KUALITAS HIDUP PASIEN KANKER PAYUDARA DI RSUP DR. KARIADI SEMARANG MENGGUNAKAN KUISIONER EORTC QLQ-C30 Marwin Marwin; Dyah Aryani Perwitasari; Susan Fitri Candradewi; Bayu Prio Septiantoro; Fredrick Dermawan Purba
Jurnal Ilmu Farmasi dan Farmasi Klinik Vol 18, No 01 (2021): Jurnal Ilmu Farmasi & Farmasi Klinik
Publisher : Universitas Wahid Hasyim Semarang

ABSTRACT Breast cancer is the most common cancer found in women and is the dominant cancer in Indonesia. Cancer treatments such as chemotherapy can affect the quality of life of patients including physical, psychological, and social. The purpose of this study is to measure the quality of life of breast cancer patients. This research used cross sectional study design. The patient quality of life was measured using the EORTC QLQ-C30 questionnaire. The subjects of this study were breast cancer patients in RSUP Dr. Kariadi Semarang meeting the inclusion criteria. The cognitive function domain, social function, emotional function, role function, and physical function were 86,15 ± 17,82; 84,10 ± 21,13; 82,56 ± 17,72; 77,95 ± 29,77; 76,72 ± 22,95, respectively. Whereas in symptom scale group from pain, fatigue, insomnia, appetite loss, financial difficulties, nausea and vomiting, constipation, dyspnea and diarrhea were 32,82 ± 30,33; 32,65 ± 21,14; 31,79 ± 34,58; 31,79 ± 32,51; 30,77 ± 32,44; 23,59 ± 25,67; 17,95 ± 30,9; 4,62 ± 14,28; 4,10 ± 13,83, respectively. For global health status / quality of life obtained 72.18 ± 18.94. The highest score on the functional scale is the cognitive function domain and the lowest score is the physical function.On the scale of symptom the domain that has the highest score is the pain domain and the lowest score is the diarrhea domain. Keywords: quality of life, breast cancer, EORTC QLQ-C30

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Jurnal Ilmu Farmasi dan Farmasi Klinik Jurnal Ilmu Farmasi & Farmasi Klinik Vol 13 No 2 Desember 2016
Publisher : Universitas Wahid Hasyim Semarang

ISSN : 1693-7899 Volume 13 No.2 Desember 2016Jurnal Ilmu Farmasi & Farmasi Klinik Journal of Pharmaceutical Science & Clinical Pharmacy Pemimpin Umum/ Penanggung jawab Dekan Fakultas Farmasi Universitas Wahid Hasyim Pemimpin Redaksi Ketua Program Studi Farmasi Fakultas Farmasi Universitas Wahid Hasyim Redaktur Ahli Dr. Sumantri, M.S., Apt Prof. Dr. SuwijiyoPramono, Apt Prof. Dr. Mustofa, M.Kes, Apt Dr. Mimiek Murukmihadi, SU., Apt Redaktur Pelaksana Devi Nisa Hidayati, S.Farm, M.Sc. Staf Redaksi : Ellya Zulfa, S.Farm., M.Sc., Apt. Dewi Andini Kunti, S.Farm., M.Farm. Kiki Damayanti, S.Farm Sekretaris Redaksi Ririn Lispita Wulandari., S.Farm., M.Si.Med., Apt Distributor Imam Asrofi Alamat Redaksi/Tata Usaha Fakultas Farmasi Universitas Wahid Hasyim Jl. Menoreh Tengah X/22 Sampangan Semarang Telp. 024-8505680, 8505681 Fax. 024-8505680 E-mail :jurnalfarmasiuwh@yahoo.co.id Terbit 2 kali setahun sejak September 2004 ISSN : 1693-7899 Volume 13 No.2 Desember 2016Jurnal Ilmu Farmasi & Farmasi Klinik Journal of Pharmaceutical Science & Clinical Pharmacy DAFTAR ISI Karakterisasi dan Aktivitas Repelen Minyak Atsiri Sereh Wangi (Cymbopogon nardus L), Akar Wangi (Vetiveria zizanoides L.), Nilam (Pogestemon cablin), Cengkeh (Syzgium aromaticum) Asal Kabupaten Garut Terhadap Nyamuk Aedes aegypti Betina Diki Prayugo Wibowo, Ardi Rustamsyah, Yunan Kurniawan..………….................... Ekstrak Daun Kelor (Moringa oleifera Lam.) Sebagai Peluruh Kalsium Batu Ginjal Secara in Vitro Yance Anas, Ali Imron, Sekar Indah Ningtyas…………………................................. 1-6 7-15 Perbandingan Metode Ekstraksi Maserasi dan Sokletasi Terhadap Kadar Flavonoid Total Ekstrak Etanol Daun Kersen (Muntingia calabura) Anita Dwi Puspitasari, Lean Syam Proyogo..…………............................................. Uji Aktivitas Imunomodulator Fermentasi Teh Hitam Jamur Kombucha Terhadap Fagositosis Makrofag Mencit Galur Balb/C Secara in Vitro Amalia Nurul ‘Ulum, Maria Ulfah, Ediati Sasmito…………………........................... Aktivitas Antioksidan dan Uji Iritasi Sediaan Masker Gell Peel-Off Ekstrak Metanol Kulit Buah Rambutan (Nephelium lappaceum L.) Reni Widaya Murti, Nabila Annisa Praditia, Hanuriza Umi Hadifa, Ratna Kurniasih, Fahmi Naqi, Rina Wijayanti………………….............................................................. Formulasi Kombinasi Pemanis Sukrosa dan Aspartam Terhadap Sifat Fisik Tablet Hisap Ekstrak Etanol Daun Pare (Momordica charantina L.) Dewi Andini Kunti Mulangsri, Wahyu Setianingsih, Mufrod…………………......... Hubungan Gaya Kepemimpinan Terhadap Produktivitas Departemen Produksi PT X Yang Dimediasi Oleh Motivasi Kerja (Studi Kasus di Bagian Tablet Departemen Produksi Salah Satu Industri Farmasi di Jawa Tengah) Indra Putra Taufani, Achmad Fudholi, dan Sumarni…………………......................... 16-23 24-31 32-38 39-45 46-52

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