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Bioeksperimen: Jurnal Penelitian Biologi
Published by Universitas Muhammadiyah Surakarta
ISSN : -     EISSN : -     DOI : -
Core Subject : Education, Agriculture,
Agriculture, Biological Sciences & Forestry Education
Arjuna Subject : -
Articles 484 Documents
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Budidaya Jakaba dan Aplikasi sebagai Insektisida terhadap Larva Ostrinia fulnacaris Guenee Kresensia Aprilia Ani; Ernawati Ernawati; Suryani Suryani; Elvi Adonis; Adolfina Manao; Redemtus Ariyana
Bioeksperimen: Jurnal Penelitian Biologi Vol 9, No 1: March 2023
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/bioeksperimen.v9i1.19826

Abstract

Cover Vol 7, No 2: September 2021 Cover Vol 7 No 2: September 2021
Bioeksperimen: Jurnal Penelitian Biologi Vol 7, No 2: September 2021
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/bioeksperimen.v7i2.16526

Abstract

Phycoremediation of Cadmium using Chlorella vulgaris in Photobioreactor Anca Awal Sembada; Teguh Adhitia Suyadi
Bioeksperimen: Jurnal Penelitian Biologi Vol 9, No 1: March 2023
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/bioeksperimen.v9i1.20316

Abstract

Chlorella vulgaris had the ability to accumulate heavy metals in their bodies, so they could be used as biosorbent in handling heavy metal pollution in waters. The effectiveness of C. vulgaris in the remediation of cadmium (Cd) would be tested in this present study. C. vulgaris were cultured for 14 days in the photobioreactor which was an enclosed chamber that was fully aerated and illuminated with LED lamps. Cadmium with concentrations of 0 (control), 0.05, and 0.1 ppm was mixed with C. vulgaris growth medium. The number of C. vulgaris cells was counted every 3.5 days using a hemocytometer to determine the growth condition. Metal concentrations were also measured on days 0, 7, and 14 using atomic absorption spectrophotometry (AAS). C. vulgaris was able to reduce cadmium levels up to 98%. The decrease in cadmium levels with the highest efficiency occurred at a cadmium concentration of 0.05 ppm.
Exploration of nyamplung rizosphere nitrogen fixing bacteria in mine reclamation Sriwulan Sriwulan; Tita Dewi Fitriani; Nadia Puspita Maya; Ni'matur Rohmah; Eko Purnomo
Bioeksperimen: Jurnal Penelitian Biologi Vol 10, No 1: March 2024
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/bioeksperimen.v10i1.23011

Abstract

Nitrogen fixing bacteria have the ability to utilize free nitrogen as a nitrogen source for their growth. These bacteria are able to make efficient availability of N- in the soil, so that it can be utilized by plants. The aim of this study was to obtain nitrogen fixing bacteria isolates from the rhizosphere of nyamplung plants in limestone reclamation lands. Isolation and testing of nitrogen fixation ability by nitrogen fixing bacteria was carried out using selective Ashby's Mannitol Agar media. The isolation results obtained 5 isolates on Ashby's Mannitol Agar media. The morphological characters of the colonies of the five isolates showed round and irregular shapes, flat and convex elevations with flat edges, white, cloudy white and clear. Microscopic observation showed that the isolates consisted of gram-positive and negative bacteria with bacilli cell shape. The catalase test showed 4 positive isolates and 1 negative. The conclusion of this study was that 5 nitrogen fixing bacteria isolates were obtained from the rhizosphere of the nyamplung limestone land plant. Isolates 1, 3, and 5 showed the character of the genus Clostridium, isolate 2 led to the genus Bacillus, and isolate 4 had the same character as the genus Klabsiella.
Chromium (Cr) Metal Absorbtion Using Symbiosis of Fimbristylis globulosa with Agrobacterium sp. Alfian Chrisna Aji; Novia Citra Paringsih; Dwi Rizaldi Hatmoko; Ilham Mujahidin; Riza Rifai; Afif Iqbal Alviandi; Almas Al Jinan; Mujahidin Mujahidin; Sofyan Anif
Bioeksperimen: Jurnal Penelitian Biologi Vol 9, No 2: September 2023
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/bioeksperimen.v9i2.16213

Abstract

Chromium (Cr) metal is a pollutant caused by industrial waste disposal and agricultural activities. An alternative solution to managing chromium pollution in an environmentally friendly manner is phytoremediation using Fimbristylis globulosa combined with Agrobacterium sp. This research aims to measure 1). the ability of Fimbristylis globulosa combined with Agrobacterium sp. to absorb Cr metal; and 2). the value of the Cr metal bioconcentration factor (BCF) absorbed by Fimbristylis globulosa combined with Agrobacterium sp. This research used factorial design 2x5 with randomized design. Chromium metal analysis using atomic absorption spectrophotometer. Data were analyzed using BCF test. The results showed: 1) the highest Cr metal absorption in the treatment of Fimbristylis globulosa-Agrobacterium sp.-Cr metal content of 30 ppm (T2V3) root: 7,987 ppm and shoot: 15,649 ppm; 2) the highest value of Cr metal BCF in the treatment of Fimbristylis globulosa-Agrobacterium sp.-Cr metal content of 30 ppm (T2V3) root is 0.266 and Fimbristylis globulosa-Agrobacterium sp.-Cr metal content 10 ppm (T2V1) shoot is 0.675. Conclusions of this research are: 1) Fimbristylis globulosa combined with Agrobacterium sp. has a higher Cr metal absorption ability than without a combination of both; and 2) Fimbristylis globulosa combined with Agrobacterium sp. has a higher value of the Cr metal BCF than without a combination of both. 
Diversity of Crustaceans Family: Portunidae in East Coast Mangrove Forest Reserve Mendahara Resort Tanjung Jabung Timur Wafiq Nadya; Mahya Ihsan; Tia Wulandari
Bioeksperimen: Jurnal Penelitian Biologi Vol 10, No 1: March 2024
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/bioeksperimen.v10i1.23163

Abstract

Crustacea, the largest subphylum in the Phylum Arthropoda, inhabits fresh and seawater. Their bodies are divided into three segments namely cephalo, thorax, and abdominal, or cephalothorax in some crustacean species and they breathe through gills. Crabs are one of the biota that can function as aquatic bioindicators in addition to making a major contribution to nutrient recycling. In the muddy parts of the mangrove forest, the decapod crustacean species Portunidae plays an important ecological function in the environment. This research aims to categorize the crustaceans of the Portunidae family in the East Coast Mangrove Forest Nature Reserve and calculate their dominance, uniformity, and diversity indices. The diversity, uniformity, and dominance index of the Crustacean Family Portunidae in the East Coast Mangrove Forest Reserve area was calculated in this quantitative descriptive research. Purposive sampling is a sampling approach used in survey methods used for sample collection. The results showed that the two different types of crustaceans collected for this study were Thalamita crenata (4125 individuals) and Scylla olivacea (a total 19 individuals). This research shows that in the East Coast Mangrove Forest Reserve area, Thalamita crenata is the most commonly found crustacean species in the Portunidae family. The overall dominance index is in the medium group, while the overall diversity and uniformity index is in the low group. The results of Principal Component Analysis (PCA) show that the environmental factor with the closest relationship and positive correlation between species diversity and uniformity and environmental parameters is salinity.
Isolation and Molecular Identification of Indole Acetic Acid-Producing Endophytic Bacteria from Daun Dewa Plant (Gynura divaricata (L.) DC) Ratna Dewi Setyowati; Artini Pangastuti; Ari Susilowati
Bioeksperimen: Jurnal Penelitian Biologi Vol 9, No 2: September 2023
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/bioeksperimen.v9i2.23003

Abstract

Indole acetic acid (IAA) is an auxin hormone that can regulate plant growth and development. Bacteria produce IAA through L-tryptophan metabolism. The purpose of this study was to determine the ability of endophytic bacteria isolated from Daun Dewa plants to produce IAA to increase the germination and growth of tomato seedlings. Isolation of endophytic bacteria was carried out by sterilizing plant surfaces. The production of IAA is done by adding L-tryptophan precursors. The results showed that there were six endophytic bacteria, and three isolates of them have the potential to produce IAA, namely: AD1.1, UD1.1, and UD2.1. The highest IAA concentration was produced by isolate AD1.1. Growth in sprouts is significantly influenced by the concentration of IAA; the higher the concentration of IAA, the longer the shoots and the roots. Based on the 16S rRNA gene sequence, these isolates belong to the genus Klebsiella.
Cytotoxic Effects of Plant Bioactive Compounds on T47d Breast Cancer Cells Line: A Review Tio Firmansyah; Utami Saraswati; Kurniadi Ilham; Robby Jannatan; rita maliza
Bioeksperimen: Jurnal Penelitian Biologi Vol 10, No 1: March 2024
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/bioeksperimen.v10i1.23534

Abstract

The continuous cell line known as T47D cells originates from the isolation of epithelial cells from the ductal breast tissue of a 54-year-old woman with metastatic breast cancer. Numerous plant species have been employed in alternative anti-breast cancer therapy, showing advancements in recent years. The aim of this systematic review is to assess the potential bioactive compounds from herbal plant extracts and analyze the effects of herbal plants on T47D breast cancer cells. This research employs a systematic approach to gather articles, encompassing related research questions, chosen databases, keyword selection, inclusion and exclusion criteria, as well as outcomes and conclusions regarding the collected articles. From the reviewed articles, several plants exhibit anti-breast cancer activity on T47D cell lines, namely Calamintha incana, Sterculia quadrifida, Psidium guajava, Uncaria nervosa, Syzygium aqueum, Capsicum annum, Bauhinia scandens, Lansium domesticum, and Garcinia cowa due to their content of bioactive compounds such as triterpenoids, alkaloids, phenolics, flavonoids, saponins, and tannins. These bioactive compounds from the plants exert significant influence on cell cycle, proliferation, fatty acid synthesis, and apoptosis of cancer cells.
Diversity, Vegetation Structure, Estimated Biomass and Carbon Stock in The Site of Limboto Lake of Gorontalo Regency Sukirman Rahim; Dewi Wahyuni K Baderan; Bella Saskia Arfa; Melisnawati H Angio; Muhammad Rifqi Hariri; Dwinda Mariska Putri
Bioeksperimen: Jurnal Penelitian Biologi Vol 9, No 2: September 2023
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/bioeksperimen.v9i2.23010

Abstract

The development of Limboto Lake  Site as the pilot area of Gorontalo Geopark is one step  in rescuing the lake. This research aims to analyzed  the vegetation structure, estimate biomass, and assess the carbon stock value of the vegetatio at the Limboto Lake site. This study employed a purposive sampling method. Important Value Index, a measure used to evaluate plant vegetation structure, was calculated based on Realtive Density, Relative Dominance, and Relative Frequency values. Biomass estimation and carbon stock assessment were conducted using allometric equations. The study’s finding showed that the vegetation surrounding Soekarno's landing area exhibited the highest IVI (sawlogs) scores for Swietenia mahagoni tree at 82.86%, Leucaena leucocephala saplings at 79.56%, and Manihot esculenta seedlings at 46.33%. Leucaena leucocephala consistently achieved the highest IVI score within the Limboto Lake area with a score as high as 76.90%, followed by Leucaena leucocephala on the sapling level with a score as high as 70.03%, and Nauclea orientalis on the seedling level with a score as high as 53.06%. Swietenia mahagoni, Samanea saman, and Leucaena leucocephala are three species with stems that predominate at the sawlog level and sapling, and these stems have high carbon absorption values.
Local Knowledge of The Community in Mekarwangi Village on Long-Tailed Macaque (Macaca fascicularis) Canaya Vania Rehansha; Rusdi Hasan
Bioeksperimen: Jurnal Penelitian Biologi Vol 10, No 1: March 2024
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/bioeksperimen.v10i1.23855

Abstract

Local knowlede is a phenomenon that is felt and experienced by the people of a particular area who have local understanding regarding the natural resources found in the surrounding environment. One of this knowledge is about long-tailed macaque (Macaca fascicularis). This research aims to examine local knowledge of the community regarding long-tailed macaque in Mekarwangi Village which borders Taman Hutan Raya Ir. H. Djuanda which is one of the long-tailed macaque conservation sites. The method used in this research is a qualitative approach through observation, semi-structured interviews, and strengthened by literature study. The result obtained were analyzed descriptively. The reseach result show that the community knows about the existence of long-tailed macaque. Community interactions with long-tailed macaque pose a threat to long-tailed macaque, some individu of long-tailed macaque be arrasted because people are disturbed by their existance. The majority of community in Mekarwangi Viilage know the food, habitat, and daily activities of long-tailed macaque from daily observation and some knowledge passed down from previous generations.

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