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Elkawnie
Published by Universitas Islam Negeri Ar-Raniry Banda Aceh
ISSN : 24608912     EISSN : 24608920     DOI : -
Core Subject : Science, Engineering,
Biochemistry, Genetics & Molecular Biology Engineering
Elkawnie is a journal of Integration Science and Technology with Islam. It's covering research and technology in the field of study of Architecture, Biology, Chemistry, Environmental Engineering, ICT, Physical Engineering and other science and technology field. In particular, Elkawnie's journal discusses the development of research and technology in contributing to development as part of Muslim scientists in the academic sphere.
Arjuna Subject : Teknik (semua kategori) - Teknik (Lain-Lain)
Articles 460 Documents
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Effect of Temperature on Yield Product and Characteristics of Bio-oil From Pyrolysis of Spirulina platensis Residue Siti Jamilatun; Yeni Elisthatiana; Siti Nurhalizatul Aini; Ilham Mufandi; Arief Budiman
Elkawnie Vol. 6 No. 1 (2020)
Publisher : Faculty of Science and Technology Universitas Islam Negeri Ar-Raniry

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22373/ekw.v6i1.6323

Abstract

Abstract : Dependence on the use of fossil fuels in Indonesia is still quite high, especially crude oil; if no new energy reserves found, it will disrupt long-term energy availability. Biofuel is a renewable energy source derived from biomass, such as the type of microalgae spirulina platensis (SP). Solid residues from SP extraction still contained high levels of protein and carbohydrates. This solid residue can be processed by pyrolysis to produce bio-oil, water phase, charcoal, and gas. Bio-oil and gas products can use as fuel, charcoal can use for pharmaceutical needs, and the water phase as a chemical can use in food and health. The pyrolysis process carried out in a fixed-bed reactor with temperature ranging from 300-600°C. Heating was carried out by electricity through a nickel wire wrapped outside the reactor. Pyrolysis product in the form of gas condensed in the condenser, the condensate formed measured by weight. Char weight measured after the pyrolysis process completed. At the same time, non-condensable gas calculated by gravity from the initial weight difference of SPR minus liquid weight (bio-oil and water phase) and char. SPR samples were analyzed proximate and ultimate, while bio-oil products examined by the GC-MS method. The experimental results showed that the optimum pyrolysis temperature at 500ºC produced by 18.45% of bio-oil, 20% of the water phase, 32.02 of charcoal, and 29.54% of gas by weight. GC-MS results from bio-oil consisted of ketones, aliphatics, nitrogen, alcohol, acids, while PAHs, phenols, and aromatics not found.Abstrak : Ketergantungan penggunaan bahan bakar fosil di Indonesia masih cukup tinggi terutama minyak mentah, jika tidak ditemukan cadangan energi baru maka akan mengganggu ketersediaan energi jangka panjang. Biofuel adalah salah satu sumber energi terbarukan yang berasal dari biomassa seperti jenis mikroalga spirulina platensis (SP). Residu padat dari ekstraksi SP masih mengandung protein dan karbohidrat yang cukup tinggi. Residu padat ini dapat diproses dengan pirolisis untuk menghasilkan bio-minyak, fase air, arang, dan gas. Produk bio-minyak dan gas dapat digunakan untuk bahan bakar, arang dapat digunakan untuk kebutuhan farmasi, dan fase air sebagai bahan kimia dapat digunakan di bidang makanan dan kesehatan. Proses pirolisis dilakukan dalam reaktor fixed-bed dengan suhu 300-600°C. Pemanasan dilakukan dengan listrik melalui kawat nikel yang dibungkus di luar reaktor. Produk pirolisis berupa gas dikondensasi dalam kondensor, kondensat yang terbentuk diukur beratnya. Berat char diukur setelah proses pirolisis selesai, sementara gas yang tidak dapat dikondensasi dihitung beratnya dari perbedaan bobot awal SPR dikurangi bobot cair (bio-oil dan fase air) dan char. Sampel SPR dianalisis proksimat dan ultimat, sedangkan produk bio-minyak dianalisis dengan metode GC-MS. Hasil percobaan menunjukkan bahwa suhu optimum pirolisis adalah 500ºC yang menghasilkan bio-oil, water phase, arang, dan gas berturut-turut adalah 18,45; 20;  32,02 dan 29,54 % berat. Hasil GC-MS dari bio-oil terdiri dari keton, alifatik, nitrogen, alkohol dan asam, sedangkan PAH, fenol dan tidak ditemukan.
Identification and Phylogenetic Analysis of Entomopathogenic Fungal Isolates Using Molecular Approach Syaukani Syaukani; Zulia Ananda; Suhartono Suhartono; Sirtina Sirtina; Oviana Lisa; Alfizar Alfizar; Samingan Samingan
Elkawnie Vol. 6 No. 2 (2020)
Publisher : Faculty of Science and Technology Universitas Islam Negeri Ar-Raniry

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22373/ekw.v6i2.6549

Abstract

Abstract: Entomopathogenic fungi are essential to consider as one of the biological agents to control termite populations. This research aimed to molecularly identify entomopathogenic fungi isolates in termites based on the ITS rDNA region and to determine the relationship of fungi isolates. Identification was performed by DNA extraction, PCR amplification, electrophoresis, purification, and sequencing. Phylogenetic trees were generated using MEGA X.  Molecular identification showed that the ISO1 sample was Penicillium oxalicum, the ISO2 sample was Trichoderma ghanense the ISO3 sample was Aspergillus niger, the ISO4 sample was Aspergillus fumigatus and the ISO5 sample was Aspergillus pseudonomius. The phylogenetic tree showed that the ISO1, ISO2, ISO3, ISO4, and ISO5 samples had the closest relationship with Penicillium oxalicum strain FR6-CGR12, Trichoderma ghanense isolate TM2, Aspergillus niger isolate 77, Aspergillus fumigatus, and Aspergillus pseudonomius strain DTO 267D6, respectively.Abstrak: Kelimpahan jenis fungi entomopatogen adalah hal yang terpenting untuk dipertimbangkan sebagai agen hayati bagi populasi rayap.Penelitian ini bertujuan untuk mengidentifikasi secara molekular isolat fungi entomopatogen pada rayap, berdasarkan daerah ITS rDNA dan mengetahui hubungan kekerabatan dari isolat fungi tersebut. Identifikasi dilakukan dengan cara ekstraksi DNA, amplifikasi menggunakan PCR, elektroforesis, purifikasi dan sekuensing. Selanjutnya kontruksi pohon filogenetik menggunakan aplikasi MEGA X. Berdasarkan uji molekular menunjukkan bahwa sampel ISO1 merupakan Penicillium oxalicum.ISO2 merupakan Trichoderma ghanense.ISO3 merupakan Aspergillus niger. ISO4 merupakan Aspergillus fumigatus.ISO5 merupakan Aspergillus pseudonomius.Konstruksi pohon filogenetik menunjukkan bahwa, sampel ISO1 berkerabat dekat dengan Penicillium oxalicum strain FR6-CGR12. Sampel ISO2 berkerabat dekat dengan Trichoderma ghanense isolat TM2. Sampel ISO3 berkerabat dekat dengan Aspergillus nigerisolat 77. Sampel ISO4 berkerabat dekat dengan Aspergillus fumigatus.Sampel ISO5 berkerabat dekat dengan Aspergillus pseudonomius strain DTO 267D6.
Characteristics and Design of Inong Balee Fort Binding Mortar For Restoration Purposes Muttaqin Hasan; Teuku Budi Aulia; Fido Yurnalis
Elkawnie Vol. 6 No. 2 (2020)
Publisher : Faculty of Science and Technology Universitas Islam Negeri Ar-Raniry

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22373/ekw.v6i2.6581

Abstract

Abstract: Inong Balee Fort is one of the Islamic Kingdom of Aceh heritage built in 1599 by Admiral Malahayati but several parts of the fortress wall have currently been damaged and the stone removed. Indonesian Government plans to restore the fort and this makes it necessary to examine the characteristics of the fortress mortar with a focus on the chemical composition and mineralogical elements. Therefore, mortar powder samples obtained from the fort walls were tested through X-Ray Diffraction (XRD) and the results showed the main composition of mortar is CaCO3 and SiO2 from a mixture of lime and sand while the others are P2O5, MgCO3, and Al2O3. Meanwhile, two mortar mixtures including 1 lime: 2 sand and 1 cement: 2 lime: 3 sand were designed for restoration purposes and they were both found by the XRD analysis results to have a diffraction pattern similar to Inong Balee Fort mortar. However, mortar with 1 lime: 2 sand has a very low compressive strength subsequently it does not meet the specifications of the SNI 6882:2014 and ASTM C270-19a while mortar with 1 cement: 2 lime: 3 sand has a compressive strength that meets the specifications. Therefore, a mortar with 1 cement: 2 lime: 3 sand is recommended to be used for the restoration of Inong Balee Fort.Abstrak: Benteng Inong Balee merupakan salah satu peninggalan Kerajaan Islam Aceh yang dibangun pada tahun 1599 oleh Laksamana Malahayati. Saat ini banyak bagian dinding pasangan batu benteng tersebut sudah rusak dan batunya sudah terlepas dari ikatan mortar. Pemerintah Republik Indonesia berencana melakukan restorasi benteng tersebut. Oleh karena itu perlu diteliti karakteristik mortar pengikat dari pasangan batu benteng tersebut, berupa kandungan senyawa kimia dan mineralnya. Metode yang digunakan untuk karakterisasi adalah dengan melakukan pengujian X-Ray Diffraction (XRD) terhadap bubuk sampel mortar yang diambil dari dinding benteng. Hasil pengujian menunjukkan bahwa komposisi utama mortar pengikatnya adalah CaCO3 dan SiO2 yang menunjukkan bahwa mortar tersebut terbuat dari campuran kapur dan pasir. Disamping itu juga terdapat kandungan senyawa P2O5, MgCO3 dan Al2O3. Selanjutnya untuk keperluan restorasi didesain 2 campuran mortar, yaitu mortar dengan campuran 1 kapur : 2 pasir dan mortar dengan campuran 1 semen : 2 kapur : 3 pasir. Hasil analisis XRD menunjukkan bahwa kedua campuran tersebut mempunyai pola diffraksi yang mirip dengan Benteng Inong Balee. Akan tetapi mortar dengan campuran 1 kapur : 2 pasir mempunyai kuat tekan yang sangat rendah sehingga tidak memenuhi spesifikasi Standar SNI 6882:2014 dan ASTM C270-19a, sedangkan mortar dengan campuran 1 semen : 2 kapur : 3 pasir mempunyai kuat tekan yang memenuhi spesifikasi Standar SNI 6882:2014 dan ASTM C270-19a, sehingga mortar ini disarankan digunakan untuk keperluan restorasi Benteng Inong Balee.
Al-Qur’an and Modern Geoscience: Part I Muhammad Zaini; Mulyadi Abdul Wahid; Abd Mujahid Hamdan
Elkawnie Vol. 6 No. 1 (2020)
Publisher : Faculty of Science and Technology Universitas Islam Negeri Ar-Raniry

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22373/ekw.v6i1.6828

Abstract

Abstract : Islamic tradition and science have been going through a long history. The Islamic world centred in the Middle East was once a centre of knowledge that was marked by the birth of prominent Islamic scientists and the development of knowledge and technology. Islamic tradition and science are naturally integrated. For instance, it can be seen from the necessity of Earth science knowledge in Islamic ritual practising. However, the decline of Muslim civilization also had an impact on science, including in the field of geoscience. This paper seeks to explain the relationship between the Tafsir al-Qur’an and geoscience, among of them are about the creation of the earth, the structure of the earth, as well as tectonic and volcanism, wherein al-Qur’an they are delivered in many parts.Abstrak : Tradisi Islam dan sains mengalami sejarah yang panjang. Dunia Islam yang berpusat di Timur Tengah pernah menjadi pusat pengetahuan yang ditandai dengan lahirnya ilmuan Islam terkemuka dan pengembangan pengetahuan dan teknologi. Tradisi Islam dan sains terintegrasi secara alamiah termasuk integrasi antara ilmu kebumian dan peribadatan ummat Islam. Namun, kemunduran Islam juga berdampak pada kemunduran di bidang sains, termasuk pada bidang kebumian. Makalah ini berupaya memaparkan hubungan antara tafsir-tafsir Al-Quran dengan ilmu kebumian, diantaranya penciptaan bumi, struktur bumi, serta tetonika dan vulkanisme, dimana hal tersebut telah disebutkan di beberapa bagian di dalam Al-Quran.
Comparison of Nata Quality From Cassava Peels (Manihot esculenta), Ladyfinger Bananas Peels (Musa acuminata Colla), and Durian Peels (Durio zibethinus) Mashuri Masri; Irhamniah Irhamniah; Ulfa Triyani A Latif; Rusny Rusny
Elkawnie Vol. 6 No. 1 (2020)
Publisher : Faculty of Science and Technology Universitas Islam Negeri Ar-Raniry

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22373/ekw.v6i1.6870

Abstract

Abstract : Agricultural waste is composed of carbohydrates which can be be used as a growth medium for bacteria Acetobacter xylinum to produce fermented products in the form of nata.  Producing nata from fruit waste such as Cassava peels (Manihot esculenta), Ladyfinger bananas peels (Musa acuminata Colla) and Durian peels (Durio zibethinus) is one of the efforts to reduce environmental pollution. This study aims to compare the quality of nata from Cassava peels (endodermis), Ladyfinger bananas peels (endodermis) and durian peels (endodermis) based on the gel thickness, nata yield and organoleptic test. From the results of the study concluded that Durian peels and Ladyfinger bananas peels could be used as an essential ingredient in making nata. Nata de durio is the best nata product in terms of thickness, nata yield and organoleptic tests. Meanwhile Cassava peels cannot be used as raw material for making nata.Abstrak : Limbah pertanian terdiri dari karbohidrat, yang dapat digunakan sebagai media pertumbuhan bagi bakteri Acetobacter xylinum untuk menghasilkan produk fermentasi dalam bentuk nata. Memproduksi nata dari limbah buah seperti kulit ubi kayu (Manihot esculenta), kulit pisang Ladyfinger (Musa acuminata Colla), dan kulit Durian (Durio zibethinus) adalah salah satu upaya untuk mengurangi pencemaran lingkungan. Penelitian ini bertujuan untuk membandingkan kualitas nata dari kulit ubi kayu (endodermis), kulit pisang emas (endodermis), dan kulit durian (endodermis) berdasarkan ketebalan gel, rendemen nata, dan uji organoleptik. Hasil penelitian menyimpulkan bahwa kulit Durian dan kulit pisang emas dapat digunakan sebagai bahan penting dalam membuat nata. Nata de durio adalah produk nata terbaik dari segi ketebalan, rendeman, dan uji organoleptik, sedangkan kulit ubi kayu tidak dapat digunakan sebagai bahan baku pembuatan nata.
The Effect of Chemical Activation Using Base Solution With Various Concentrations Towards Sarulla Natural Zeolite Saharman Gea; Agus Haryono; Andriayani Andriayani; Junifa Layla Sihombing; Ahmad Nasir Pulungan; Tiamina Nasution; Rahayu Rahayu; Yasir Arafat Hutapea
Elkawnie Vol. 6 No. 1 (2020)
Publisher : Faculty of Science and Technology Universitas Islam Negeri Ar-Raniry

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22373/ekw.v6i1.6913

Abstract

Abstract : Material characteristics analysis of Sarulla natural zeolite (SNZ) with base activation has been carried out. The base used was NaOH at various concentrations; 0.2 M, 0.5 M, and 2 M. Base activated catalysts were characterized by X-ray Fluorescence (XRF), X-ray diffractometer (XRD), Fourier-transform Infrared (FTIR) Spectroscopy, Scanning Electron Microscopy (SEM) and gas adsorption analysis using BET method. The procedures applied have caused differences in adsorption and desorption rates as well as the crystallinity values of the catalytic materials. Moreover, the ratio of Si/Al contents has also changed at the lowest concentration. On the other hand, the crystallinity of catalysts has decreased at the highest concentration of base applied. The results are reinforced in FTIR characterization which shows the changes of silanol bonds to become silicate and aluminate. Furthermore, morphological analysis of the catalysts shows that homogenous surface was obtained at low concentration, while rough/lumpy surfaces was obtained at higher concentration.Abstrak : Analisis terhadap karakteristik material dari zeolit alam Sarulla (SNZ) dengan metode aktivasi basa telah dilakukan. Basa yang digunakan ialah NaOH dengan berbagai konsentrasi; 0,2 M; 0,5 M, dan 2 M. Katalis yang telah diaktivasi dengan basa kemudian dikarakterisasi dengan Analisis X-ray Fluorescence (XRF), X-ray diffractometer (XRD), Fourier-transform Infrared (FTIR) Spectroscopy, Scanning Electron Microscopy (SEM) dan Gas Adsorption menggunakan metode BET. Dari hasil penelitian, ditemukan perbedaan pada tingkat adsorpsi dan desorpsi, begitu juga dengan kristalinitas pada material katalis. Lebih jauh, rasio konten Si/Al juga berubah pada basa dengan konsentrasi terendah. Di sisi lain, tingkat kristalinitas dari katalis mengalami penurunan pada basa dengan konsentrasi tertinggi. Hasil ini diperkuat dengan data FTIR yang menunjukkan perubahan ikatan silanol menjadi silikat dan aluminat. Lebih jauh, analisis morfologi pada katalis menunjukkan bahwa permukaan yang homogen didapatkan dari penggunaan basa dengan konsentrasi rendah. Sebaliknya, basa dengan konsentrasi tinggi memberikan bentuk permukaan katalis yang tidak homogen serta kasar.
The Prevention of Ulcers Decubitus with Mobilization and The Usage of Olive Oil on Stroke Patients Siti Cahaya Meliza; Kiking Ritarwa; Nur Asnah Sitohang
Elkawnie Vol. 6 No. 2 (2020)
Publisher : Faculty of Science and Technology Universitas Islam Negeri Ar-Raniry

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22373/ekw.v6i2.6925

Abstract

Abstract: Decubitus ulcers are one of the problems experienced by bed rest patients, such as stroke patients, caused by immobilization. Prevention of decubitus ulcers can be done with mobilization and topical application of olive oil. Olive oil contains saturated fatty acids, unsaturated fatty acids, vitamin E, and phenols which are healthy for the skin. This research aims to identify the effects of mobilization and application of olive oil on the prevention of decubitus ulcers in stroke patients. A control group pretest-posttest quasi-experimental design was used in the research. Convenience (non-probability) sampling was also carried out, resulting in 64 research samples. Mobilization intervention of right-left oblique positions and application of olive oil on the area prone to decubitus ulcers were then executed for seven days. Data analysis using the Wilcoxon signed-rank test showed the effects of mobilization and application of olive oil on the preventions of decubitus ulcers in stroke patients with a value of P= <0,05. Abstrak: Ulkus dekubitus merupakan salah satu masalah yang dialami pasien tirah baring seperti pasien stroke, dengan faktor penyebabnya adalah immobilisasi, tindakan pencegahan ulkus dekubitus dapat dilkukan dengan mobilisasi dan pemberian bahan oles seperti minyak zaitun, minyak zaitun kandungannya terdiri dari asam lemak jenuh, asam lemak tak jenuh, vitamin E dan fenolik yang baik untuk kesehatan kulit. Penelitian ini bertujuan untuk mengidentifikasi pengaruh mobilisasi dan penggunaan minyak zaitun terhadap pencegahan ulkus dekubitus pada pasien stroke. Penelitian menggunakan desain Quasi-experimental dengan pretest- posttest with countrol gruopdesign.Convenience (Non-probability) dilakukan untuk pengambilan sampel, jumlah sampel penelitian 64. Intervensi mobilisasi miring kanan dan kiri kemudian bagian yang akan terjadi ulkus dekubitus diolesi minyak zaitun,intervensi dilakukan selama tujuh hari. Analisa data wilcoson signed -rank test menunjukan adanya pengaruh mobilisasi dan penggunaan minyak zaitun terhadap pencegahan ulkus dekubitus pada pasien stroke dangan nilai P= <0,05. Mobilisasi miring kanan dan kiri penggunaan minyak zaitun dapat mencegah ulkus dekubitus.
Zonation Drives The Abundance of Understory Exotic Plant Species in Ir. Djuanda Forest Park, West Java Tri Cahyanto; Billy Nabil Yuni; Muhammad Efendi
Elkawnie Vol. 7 No. 1 (2021)
Publisher : Faculty of Science and Technology Universitas Islam Negeri Ar-Raniry

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22373/ekw.v7i1.6950

Abstract

Abstract: The understory is an important component in the tropical forests, particularly to contribute to ecosystem services function and playing on succession. However, the study on their existence related to the zonation effect in an ex-situ conservation is still lacking. This study aimed to compare the structure and composition of the understory in the two blocks of Ir. Djuanda Forest Park, Bandung. Data collection was carried out through vegetation analysis using plot methods (sampling plots). A total of 40 sampling plots of 5m x 5m were made in two observation blocks. The Importance Value Index (IVI) for each species was calculated based on their relative density and relative frequency. Seventy-three species of understory from 38 families were found in the observation plots, dominated by Araceae and Moraceae families. The composition of the protected block has higher species richness than the utilization block due to the differences in microclimates conditions. Calliandra calothyrsus, known as an invasive species, has the highest IVI indicating high adaptability to open habitats in the utilization block, while two native species, Plectranthus sp. and Chlorathus elatior, dominate in the protection block. Based on these findings, we showed that forest zonation drives exotic and native species abundance in the ex situ conservation area.Abstrak: Tumbuhan bawah merupakan salah satu komponen penting dalam vegetasi hutan tropis, terutama dalam pelayanan ekosistem dan berperan dalam proses suksesi. Namun, penelitian mengenai keberadaannya dikaitkan dengan pengaruh zonasi di kawasan konservasi secara ex situ masih jarang. Tujuan penelitian ini untuk membandingkan struktur dan komposisi tumbuhan bawah pada dua blok yang berbeda di kawasan Taman Hutan Raya Ir. Djuanda, Bandung. Pengambilan sampel menggunakan analisis vegetasi dengan metode plot (petak contoh). Sebanyak 40 plot kecil berukuran 5mx5m di kedua blok pengamatan. Indeks nilai penting (INP) setiap jenis dihitung berdasarkan kerapatan relatif dan frekuensi relatifnya. Sebanyak 73 jenis dalam 38 suku tumbuhan didata di dalam pengamatan, yang didominasi dari suku Araceae dan Moraceae. Berdasarkan jumlah jenis tumbuhan penyusunnya, blok perlindungan memiliki jenis yang lebih banyak dibandingkan dengan blok pemanfaatan berkaitan dengan berkaitan dengan perbedaan kondisi iklim mikro di kedua blok tersebut. Jenis Calliandra calothyrsus, dikenal sebagai tumbuhan invasif, memiliki nilai INP tertinggi menunjukkan kemampuan adaptasi yang tinggi pada habitat terbuka di blok pemanfaatan, sedangkan tumbuhan asli pegunungan jawa, Plectranthus sp. dan Chloranthus elatior mendominasi pada blok perlindungan. Berdasarkan hasil temuan ini, kita menyarankan bahwa zonasi mempengaruhi kelimpahan tumbuhan eksotik dan asli di kawasan konservasi tumbuhan secara eksitu.
Isolation and Identification of Lactic Acid Bacteria on Boiler Chicken Roisu Eny Mudawaroch; Setiyono Setiyono; Lies Mira Yusiati; Edi Suryanto
Elkawnie Vol. 6 No. 2 (2020)
Publisher : Faculty of Science and Technology Universitas Islam Negeri Ar-Raniry

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22373/ekw.v6i2.7015

Abstract

Abstract: The aims of this research were to isolate lactic acid bacteria from broiler chicken. The main material was a chicken boiler aged 30 days. The identification of LAB was carried out based on isolation and identification of morphological, phenotypic, and biochemical characteristics. Thirteen LAB isolates were obtained and 5 selected LAB isolates were identified based on growth levels, pH, and lactic acid levels. Five selected LAB isolates were identified as Lactobacillus genus which has Gram-positive, negative catalase, non-motility, hetero-fermentative, rod shapes,  rod-colony shapes, and cream colony color.Abstrak: Penelitian ini bertujuan untuk mengisolasi bakteri asam laktat dari ayam broiler. Bahan utamanya adalah ayam boiler berumur 30 hari. Identifikasi BAL dilakukan berdasarkan isolasi dan identifikasi karakteristik morfologi, fenotipik, dan biokimia. Tiga belas isolat BAL diperoleh dan 5 isolat BAL terpilih diidentifikasi berdasarkan tingkat pertumbuhan, pH, dan kadar asam laktat. Lima isolat BAL terpilih diidentifikasi sebagai genus Lactobacillus yang memiliki Gram-positif, katalase negatif, non-motilitas, hetero-fermentatif, bentuk batang, bentuk koloni batang, dan warna koloni krem.
Metarhizium brunneum Petch Fungi is Effective as a Bioinsecticide Against Coptotermes curvignathus Holmgren Termite Pests in the Laboratory Muhammad Sayuthi; Teguh Santoso; Iswadi Iswadi
Elkawnie Vol. 6 No. 2 (2020)
Publisher : Faculty of Science and Technology Universitas Islam Negeri Ar-Raniry

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22373/ekw.v6i2.7199

Abstract

Abstract: Nutmeg is a plantation crop and has high economic value. Currently, the cultivation of nutmeg is experiencing many obstacles. One of them is termite pests. So far, the control is carried out using chemical insecticides and hurts the environment. Therefore it is necessary to control other environmentally friendly ways, and one of them is by using the fungi M. brunneum as an ecologically friendly bioinsecticide. The purpose of this study was to analyze the effectiveness of M. brunneum as a bioinsecticide against the termite C. curvignathus as an essential pest in nutmeg plantations. This research using a completely randomized design (CRD). The results showed that the mean percentage viability of M. brunneum conidia at each observation time was classified as useful. The germination of conidia increased to 89.78 % after 72 hours of observation. Conidia density 103/ mL distilled water. The highest mortality rate of C. curvignathus was found at a density of 1010 conidia/ mL of distilled water. The mean time of death for C. curvignathus after application of conidia density treatment of 1010/ mL of distilled water was 2.15 days and the death of C. curvignathus was 3.35 days using conidia density treatment 103/ mL of distilled water. 1 day after application reaches 33.20 % and increases to observation at 5 days after application reaches 100 %. The highest food inhibition occurred in treatment 1010/ mL distilled water (65.81 %) which was significantly different from treatment 105/ mL distilled water (43.23 %), and 103/ mL distilled water (41.61 %) and control (0.00).Abstrak: Pala merupakan tanaman perkebunan dan bernilai ekonomi tinggi. Pada saat ini budidayanya mengalami banyak hambatan. Satu diantaranya adalah serangan hama rayap. Selama ini  pengendalian hama rayap dilakukan dengan menggunakan insektisida kimia dan berpengaruh negatif terhadap lingkungan. Oleh karenanya perlu pengendalian cara lain yang ramah lingkungan dan satu diantaranya dengan menggunakan cendawan M. brunneum sebagai bioinsektisida yang ramah lingkungan. Tujuan dari penelitian ini adalah menganalisis efektivitas M. brunneum sebagai bioinsektisida terhadap hama rayap C. curvignathus. Penelitian ini dilaksanakan di Laboratorium Hama Tanaman dan Laboratorium Dasar Proteksi Tanaman Program Studi Proteksi Tanaman Fakultas Pertanian Universitas Syiah Kuala, sejak bulan Februari hingga September 2018, dengan menggunakan Rancangan Acak Lengkap (RAL). Hasil penelitian menunjukkan bahwa persentase viabilitas rata-rata dari konidia M. brunneum pada setiap waktu pengamatan tergolong efektif. Perkecambahan konidia semakin meningkat hingga mencapai 89,78 % setelah diamati pada jam yang ke 72. Rata-rata waktu kematian rayap C. curvignathus setelah aplikasi perlakuan kerapatan konidia 1010/ mL akuades adalah 2,15 hari dan terjadi kematian rayap C. curvignathus 3.35 hari dengan menggunakan perlakuan kerapatan konidia 103/ mL akuades. Rata-rata mortalitas tertinggi rayap C. curvignathus ditemukan pada perlakuan kerapatan konidia 1010/mL aquades. 1 HSA mencapai 33,20% dan meningkat hingga pengamatan pada 5 HSA mencapai 100%. Daya hambat makan tertinggi terjadi pada perlakuan 1010/ mL aquades (65,81%) yang berbeda nyata dengan perlakuan 105/mL aquades (43,23%) dan 103/ mL aquades (41,61%) serta kontrol (0,00%).

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