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Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences
Published by Universitas Brawijaya
ISSN : 20875517     EISSN : 25274376     DOI : -
Core Subject : Agriculture, Social,
Agriculture, Biological Sciences & Forestry Environmental Science
The Journal of Tropical Life Science (JTLS) provides publication of full-length papers, short communication and review articles describing of new finding or theory in living system, cells and molecular level in tropical life science and related areas. The journal publishes articles that report novel findings of wide Tropical Life system phenomenon in the areas of biodiversity, agriculture, fisheries, health, husbandry, forestry and environmental technology. JTLS has 1 volume with 3 issues per year.
Arjuna Subject : -
Articles 1,001 Documents
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Changes in Organic, Inorganic contents, Carbon Nitrogen ratio in decomposingAvicennia marinaand Rhizophora mucronataleaves on tidal mudflats in Hajambro creek, Indus delta, Pakistan Zafar Farooqui; Pirzada Jamal Siddiqui; Munawwer Rasheed
Journal of Tropical Life Science Vol. 4 No. 1 (2014)
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Abstract

Leaf decomposition of Avicennia marina (Forskål) Vierh in Denkschr and Rhizophora mucronata (Poiret) was studied in situ using litterbags in Hajambro creek, Indus deltA. A single exponential model was presented, which best described the rate of decomposition for both the species. The rate of decomposition was species specific, A. marina leaves decomposed slower than the R. mucronata (p
Assessment of vegetative Phenology with respect to leaf elongation pattern of Avicennia marina and Rhizophora mucronata in Hajambro creek, Indus delta, Pakistan Zafar Farooqui; Pirzada Jamal Siddiqui
Journal of Tropical Life Science Vol. 4 No. 2 (2014)
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Abstract

Leaves emergence and the rate of elongation have been studied in Hajambro creek in Indus deltaic region, Pakistan. Leaves emergence was found higher during the summer months (mostly pre-monsoon period), that was March April, May and June. Leaves of A. marina that were observed from March to July, till the leaves reached their maximum length (about 77 mm), showed a gradual increase in their length, reaching maximum in April. Leaves of Avicennia marina reached their maximum length (about 77 mm) in about 113 days. The leaves showed gradual increase in their length, reaching maximum in April (0.97mm d-1). The per-day increment in length was 0.54 mm d-1. As reaching their maximum length (77mm), the per-day increment in the length gradually slower down. The leaves of Rhizophora mucronata were observed from February to November. The leaves of Rhizophora mucronata showed elongation rate of 0.24 mm d-1 from the day of the first observation till the leaves reached their maximum length (120 mm). The maximum time required by the leaves to reach their maximum length (120 mm) was 236 days. The rates of elongation of leaves are also fast in summer. The rate of elongation of the leaves was found to be highly correlated with the temperature (p
Black Kite Populations Are Suffering Declining Trends In Kurukshetra And Likely To Get Further Depleted: An Analysis Of Causes Tirshem Kumar Kaushik; Rohtash Chand Gupta
Journal of Tropical Life Science Vol. 4 No. 1 (2014)
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Abstract

Black Kite Milvus migrans was seen capturing and captivating everything nearby the offal and garbage sites in Kurukshetra environs until recent past. Today it has been observed that between 2000 and 2010, its depletion is speeding fast, although not noticed in the scientific and social world. The skies in late morning hours are empty. Garbage sites demonstrate the absence of criss-crossing flights of this Kite. The popular sites of the roosting in the shape of Peepal Ficus religiosa, Banyan Ficus benghalensis and Mango Mangifera indica trees have been cut down in the first place. In Kurukshetra, the prime reason for the depletion could be attributed to destruction of foraging grounds (Elimination of garbage dumping sites), cutting down of traditional roosting and nesting sites and construction of colonies in the nearby erstwhile agriculture fields. The contiguous agriculture fields are devoid of any traditional trees like Peepal, Mango and hence no roosting and nesting places. The soaring Kites in the high skies in the forenoon and afternoon sessions were more or less absent. So much so that huge Garbage sites at Panipat Railway compound had no Black Kites. Shockingly, it seems that like Vultures, Black Kites are on their way to elimination in Haryana.
Endophytic Bacteria as Biocontrol Agents of Tomato Bacterial Wilt Disease Arika Purnawati
Journal of Tropical Life Science Vol. 4 No. 1 (2014)
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Abstract

Ralstonia solanacearum is one important pathogen on tomato because it caused wilt disease that can loss yield until 100%. The object of these experiment are to select of endophytic bacteria that was isolated from healthy tomato stems, root and to investigate of them as biocontrol agents for tomato bacterial wilt disease. There are 10 isolates successfully isolated and two isolates are Ps1 and Ps8 can inhibit of R. solanacearum. The result show the two isolates are Ps1 and Ps8 significantly suppress disease incidence until 8.07-9.19%.
The Effect of Calcusol™ to the Plasma Free Radical and Serum Creatinin in Mus Musculus Nephrolithiasis Model A’liyatur Rosyidah; Sri Widyarti; Sri Rahayu
Journal of Tropical Life Science Vol. 3 No. 3 (2013)
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Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.%x.%x.%x

Abstract

Calcusol is a traditional medicine (jamu) ade from Tempuyung (Sonchus arvensis) extract and is usually used for cure kidney stone disease. However, there has not been any studies which investigate the its mechanism. The aim of this study is to know the effect of Calcusolâ„¢ to the plasma free radical and serum creatinin of Mus musculus model for renal calcium-oxalate accumulation. This study is carried out by administration of Porang (Amorphophallus muelleri) tuber flour for 3 months with the doses 6 mg/100g BW to induce renal calcium oxalate accumulation and Calcusolâ„¢ treatment for 7 days with the doses of 3.3mg/g BW. Group I was used as a control group. Group II was only given porang (A. muelleri)every day for 3 months. Group III was only given Calcusolâ„¢ for 7 days. Group IV were given porang (A. muelleri) for 3 months then given Calcusolâ„¢ for 7 days. Group V were given porang (A. muelleri) and Calcusolâ„¢ simultaneously for 3 months. Porang and CalcusolTM is administrated orally. Blood was collected from the tail of theanimal for serum creatinin test and plasma free radical test using TBARS method. The data was analyzed using ANOVA followed by Tukey HSD to compare the means employing SPSS 16.0 forwindows. The result of the research shows that the treatment Calcusolâ„¢ on mice model for renal calcium oxalate accumulation. The content of MDA at group I, group II, group III, group IV and group V, respectivelyis 0.81±0.5 mg/ml; 2.63±0.8 mg/ml; 0.56±0.5 mg/ml; 2.09±0.9 mg/ml and 0.17±0.17 mg/ml. And the content of serum creatinine are 0.15±0.04 mg/dL; 0.13±0.03 mg/dL; 0.12±0.08 mg/dL; 0.11±0.016 mg/dL and 0.14±0.015 mg/dL at group I, group II, group III, Group IV, and group V respectively. This indicates that Calcusolâ„¢ decreases plasma free radical production during renal stone formation, while serum creatinin reduced but not significantly changed. Calcusolâ„¢ has an effect to decrease free radical during renalstone formation inmice model for calcium oxalate accumulation and also decrease serum creatinin.
Haplotype Diversity of COI Gene of Hylarana chalconota Species Found at State University of Malang Dian Ratri Wulandari; Ibrohim Ibrohim; Dwi Listyorini
Journal of Tropical Life Science Vol. 4 No. 1 (2014)
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Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.4.1.%x

Abstract

Hylarana chalconota is a cryptic species of frog endemic to Java Island . This species is small with long legs, and brown skin. The Snout-Vent Length (SVL) ranges between 30-40 mm for male and 45-65 mm for female. Reports the existence of this species in State University of Malang, which was not found in 1995. To reveal the haplotype diversity of COI gene in this species, we analyzed Cytochrome-c oxidase subunit-1 (COI) sequences of both samples. The both samples had 604 bp and 574 bp fragment length, respectively. These fragments showed polymorphism; with mutation position in sites 104, 105, and 124. Based on this result, we suggest that the two samples share a different haplotypes, proposed as UM1 and UM2
Classification of Fruit Flies In South Kalimantan Based on Morphology and Molecular Characters Muhammad Indar Pramudi; Retno Dyah Puspitarini; Bambang Tri Rahardjo
Journal of Tropical Life Science Vol. 3 No. 3 (2013)
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Abstract

The result of identification from assemblies trapped by methyl eugenol and collection of fruit affected fruit flies at all location, there are seven specieses of fruitfly in all locations. Based on morphological identification, which analyzed UPGMA by using the CLAD 97 program, shows that the seventh species remain one group that consists of 5 sub groups. On the other hand, according to RAPD result, which analyzed UPGMA based on 20 DNA band characters, shows that the seventh species divided into two groups. The first group consists of B. umbrosa Fabricius, B. occipitalisis Bezzi, B. latifrons Hendell sub groups. The second group consists of Bactrocera carambolae Drew and Hancock, B. papayae Drew and Hancock, and B. albistrigata de Meijere, B. cucurbitae Coquillet sub groups. Sequencing result shows that homology of seventh fruitfly species, is 83 base pairs/bp (C), 101 bp (T), 265 bp (G), 420 bp (A), 432 bp (T), 600 bp (A) (appendixes 7). The length base pair for B. occipitalis, B. cucurbitae, B. albistrigata, B. carambolae, B. papayae, B. latifrons row is 615, 898, 570, 969, and 615 bp. An analysis result of morphology feature and RAPD method show distinction in group division and sub group, but the identification morphological and DNA of the seventh species fruitfly which is found are the similar.Keywords: Fruitfly, UPGMA, Morphoogy, RAPD, DNA
The Effectiveness o f Entomopathogenic Fungi Beauveria bassiana with the Addition of Insect Growth Regulator Lufenuron f or Controlling Bactrocera carambolae Mochammad Syamsul Hadi; Toto Himawan; Luqman Qurata Aini
Journal of Tropical Life Science Vol. 3 No. 3 (2013)
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Abstract

The study of the effectiveness of entomopathogenic fungus Beauveria bassiana (Bals) Vuill. with the addition of an insect growth regulator (IGR) lufenuron to control the fruit fly Bactrocera carambolae (Drew and Hancock) (Diptera: Tephritidae) was conducted at the Laboratory of Toxicology and Laboratory of Entomology, Department of Plant Pests and Diseases, Faculty of Agriculture, University of Brawijaya Malang from March 2012 until December 2012. This study examined the effect of lufe-nuron to the development and sporulation of B. bassiana and the effectiveness of the fungus B. bassiana in combination with several concentrations of lufenuron (0.5, 1, 1.5 mL/L) on the mortality of fruit fly larvaB. carambolae. The results showed that the addition of lufenuron at the concentration of 1 mL/L could significantly increase the sporulation of B. bassiana. The addition of B. bassiana at 108 spores/mL combined with lufenuron at the concentration of 1 mL/L applied to compost pupation medium of larva of B. carambolae is significantly able to suppress the formation of the fruit fly pupa and imago of B. carambolae
Study of phytochemical, antioxidant, antimicrobial and anticancer activity of Berberis Aristata. Basanta Lamichhane; Sandeep Adhikari; Pritish Shrestha; Bhupal Govinda Shrestha
Journal of Tropical Life Science Vol. 4 No. 1 (2014)
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Abstract

The present study was undertaken to find the phytochemical presence, antioxidant potential, antimicrobial activity, and anticancer activity of methanol extract of Berberis Aristata. Plant extract showed the presence of antioxidants like alkaloid, saponin, terpenoids, coumarin, flavonoids, tannin, glycoside and steroid. Further, antioxidant assay like DPPH (Diphenyl-2-picrylhydrazyl) assay, hydrogen peroxide assay and reducing power assay was done. DPPH free radical Scavenging Activity was expressed in % inhibition with L Ascorbic acid as standard showing IC50 9.6μg/ml and that of extract was 33.31μg/ml. Hydrogen peroxide radical scavenging activity was comparable to standard, IC50 for L Ascorbic acid is 54.23µg/ml and that of B Aristata is 60.6µg/ml. Similarly, reducing power of plant extract at different concentration was comparable with L-Ascorbic Acid. Antimicrobial screening showed good positive result with Candida Albicans, Salmonella typhii, Pseudomonas aeruginosa and Escherichia coli. Cell viability assay; MTT; showed a significant cytotoxicity to MDA-MB-231 and U-87 MG human cancer cell line compared to NIH/3T3 standard embryonic fibroblast cell lines of mouse.
Toll Like Receptor 4 (Tlr4) And p65 Nuclear Factor Kappa B (Nf-Kb) Expression In Monocyte Cell Of Children With Steroid Resistant Nephrotic Syndrome Tikto Wahyono; Krisni Subandiyah; Loeki Enggar Fitri; Agustina Tri Endharti
Journal of Tropical Life Science Vol. 4 No. 2 (2014)
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This study aimed to prove the difference of TLR4 and subunit p65NF-kB expression between children with steroid-sensitive nephrotic syndrome (SSNS) and those with steroid-resistant nephrotic syndrome (SRNS) and to find out the correlation between expression of TLR4 and p65NF-kB on children with SRNS. Twenty three patients of idiopathic nephrotic syndrome patients (INS) were enrolled in this study and classified into two groups—those with SSNS (11 patients) and SRNS (12 patients). Peripheral Blood Mononuclear Cells (PBMC) isolation was performed, taken from 5 ml patients blood samples, followed by monocyte isolation of PMBC using monocyte culture. Examination of TLR4 and p65 NF-kB expression from monocyte's cells culture was performed using flow cytometry. All data were processed using Statistical Package for the Social Sciences (SPSS 15) for Windows. From 11 patients that were classified into SSNS group, 81.8% having good nutritional status and 18.2% comprising undernourished status compared to 12 patients of the SRNS group, in which 6 accounting for 41.7% having good nutritional status, 50% having undernourished status and 8.3% having malnourishment status. The average age is 7.59 ± 3.52 and six years old is the highest age studied. Based on normality tests using Shapiro-Wilk, variables of TLR4 have p value of 0.620 and p65 NF-kB have p value of 0.027, showing that the data of TLR4 was distributed normally but p65 NF-kB data was not distributed normally. The test on TLR4 data variation using Levene Test showed its significance value of p was 0.318, meaning that the data was homogeneous. Using unpaired t-test, the differences of TLR4 expression between SSNS and SRNS was significant (p=0.012), while using Mann-Whitney test, the differences of p65 NF-kB expression between SSNS and SRNS showed an insignificant correlation (p=0.880). Pearson correlation test between the expression of TLR4 and p65 NF-kB showed no significant correlation (p=0.472), while Spearman correlation test to see the relationship between expression of TLR4 and SRNS produced correlation value of 0.512 and its importance p value was 0.013 (p

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