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INDONESIA
Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences
Published by Universitas Brawijaya
ISSN : 20875517     EISSN : 25274376     DOI : -
Core Subject : Agriculture, Social,
Agriculture, Biological Sciences & Forestry Environmental Science
The Journal of Tropical Life Science (JTLS) provides publication of full-length papers, short communication and review articles describing of new finding or theory in living system, cells and molecular level in tropical life science and related areas. The journal publishes articles that report novel findings of wide Tropical Life system phenomenon in the areas of biodiversity, agriculture, fisheries, health, husbandry, forestry and environmental technology. JTLS has 1 volume with 3 issues per year.
Arjuna Subject : -
Articles 1,001 Documents
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Inhibition of Klebsiella pneumoniae adhesion in mice enterocytes by antibodies of hemagglutinin pili protein with MW 12.8 kDa of Klebsiella pneumoniae Dini Agustina; Sumarno Retoprawiro; Noorhamdani AS
Journal of Tropical Life Science Vol. 4 No. 1 (2014)
Publisher : Journal of Tropical Life Science

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Abstract

Klebsiella pneumoniae as one of the most common cause of VAP is also the second most common cause of community- and hospital-acquired gram negative bloodstream infection. The process of infection caused by direct contact with infectious agents begins with the host cell adhesion process either by pili or by afimbria adhesin (AFA). There were no reported researches on the hemagglutinin pili protein of K.pneumoniae as adhesion factors.This study was conducted in order to determine the hemagglutinin pili protein of K.pneumoniae, polyclonal antibody produced from pili protein immunization, and its ability to inhibit K.pneumoniae adhesion in mice enterocytes. Adhesion inhibition test used HA antibody with the implementation of dose dilutions of 1/100, 1/200, 1/400, 1/800, 1/1600, 1/3200 and 0 (control). While immunocytochemistry test used HA pili protein with the implementation of dose dilutions of 1/10000, 1/20000, 1/40000, 1/80000, 1/160000, 1/320000 and 0 (control). Hemagglutinin pili protein found in K.pneumoniae had MW 12.8 kDa. Pearson correlation analysis of adhesion test showed there were significant correlation between antibody dilution titer with bacterial adhesion (p = 0.032, R = -0.797). Furthermore, Anova analysis of IT showed that there were significant differences between the various dilution titer with antigen-antibody reaction (p=0.000). Antibody of hemagglutinin pili protein with MW 12.8 kDa of K.pneumoniae can inhibit the adhesion of K.pneumoniae to the enterocytes of mice.
The Effect of Different Orthodontic Force on MMP 9 Expression in a Rat Diabetic Model Hafiedz Maulana; Nuzulul Hikmah; Amandia Dewi permana Shita; Nur Permatasari; Sri Widyarti
Journal of Tropical Life Science Vol. 4 No. 2 (2014)
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Abstract

Orthodontic forces produce alveolar bone and periodontal ligament remodeling that cause tooth movement. Diabetes can increase tissue damage and, therefore, contributes to the process of orthodontic tooth movement. The aim of this study was to determine the effect of differently orthodontic forces on the expression of MMP nine in rat diabetic model. Twenty four male Wistar rats were divided into three groups of control and three groups of treatment streptozotocin-induced diabetes with stratified doses. Application of orthodontic appliance was performed with different forces (10, 20 and 30 grF). Orthodontic appliance was performed on both upper incisors. Immunohistochemical (IHC) analysis was used to observe the expression of MMP 9 and HE (Hematoxylen Eosin) staining to observe the number of fibroblasts. Statistical analysis was carried out using t-test and Spearman correlation analysis to determine the difference of MMP nine expression and number of fibroblasts between groups, and determine the correlation of both. The results showed an increased expression of MMP 9 and decreased of fibroblasts number in diabetic rats, along with increasing magnitude of orthodontic forces. The different orthodontic forces given to diabetic rats affect the expression of MMP 9 and the number of fibroblasts, in which the expression of MMP 9 increased along with the increase of orthodontic forces, both at pressure and tension sides.
Effect of Tetrodotoxin from Crude Puffer Fish (Tetraodon fluviatilis) Liver Extract on Intracellular Calcium Level and Apoptosis of HeLa Cell Culture Natanael Untario; Titik Cinthia Dewi; M. Aris Widodo; Pudji Rahaju
Journal of Tropical Life Science Vol. 7 No. 1 (2017)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.07.01.04

Abstract

Cervical cancer is the third most commonly diagnosed cancer and fourth leading cause of women death with 8% of total death caused by cancer in women in 2008. Tetrodotoxin (TTX) is a potent neurotoxin found in inner organs puffer fish, with the specific mechanism of sodium channel blocking, and widely used for research purposes. Previous reports claimed that TTX has the capability of inhibiting the metastatic process of cancer and apoptotic effect. Studies also show that apoptosis is a process involving the increase of intracellular calcium level, yet the connection between TTX and increase of intracellular calcium level, therefore triggering apoptosis, has not been established. This is an experimental study with post test only control group design, carried out by exposing HeLa cell culture to a crude liver extract of a puffer fish species, Tetraodon fluviatilis. Crude puffer fish liver extract is administered into HeLa cell culture well in different concentrations 10-4, 10-2, and 10-1. Intracellular calcium level and apoptosis were then measured after 18 hours of incubation. Measurements of intracellular calcium level were done by using CLSM with Fura-2AM staining, and apoptosis by using flowcytometry with Annexin V/PI.  The result shows that there is a significant difference between samples both in intracellular calcium (p < 0.05) and apoptosis (p < 0,05). Both intracellular calcium and apoptosis levels are proportional to liver fish extract concentration. Pearson’s correlation test shows correlation between treatment and intracellular calcium levels (p = 0.000), between treatment and apoptosis (p = 0.002), but not between intracellular calcium and apoptosis (p = 0.05). These results suggest that TTX induces an increase in intracellular calcium level and apoptosis, but calcium pathway is not the sole cause of the apoptosis. 
Dose Optimization of Calcusolâ„¢ and Calcium Oxalate Monohydrate (COM) on Primary Renal Epithelial Cells Cultures of Mice (Mus musculus) Ahmad Soni; Muhammad Sasmito Djati; Sri Widyarti
Journal of Tropical Life Science Vol. 4 No. 2 (2014)
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Abstract

Kidney stones are one of the urologic diseases that have plagued mankind for centuries. The main constituents of stones in the kidney are calcium oxalate monohydrate (COM) crystals. Nowadays, there are varieties of drugs and treatments that can be made to minimize the grievances due to kidney stone disease. The treatment can be done either by using chemicals or traditional medicine. Calcusolâ„¢ is one of the popular herbal products that have been used by Indonesian people in curing the kidney stone disease. The main constituent that was contained in Calcusolâ„¢ is an extract of the tempuyung leaves (Sonchus arvensis L.), which is expected could cure the kidney stone disease. This study used primary cultured renal epithelial cells of mice to determine the optimal dose of Calcusol and the optimal dose of COM. The primary Kidney epithelial cell were treated with Calcusolâ„¢ and COM at various doses. The analysis of the cell death either by necrosis or apoptosis pathways was analyzed by flow cytometric analysis. The results that were obtained is the percentage of cell death that is then analyzed by using a complete randomized design (CRD) One Way Anova. Based on the results that were obtained, it is known that the optimal dose of Calcusolâ„¢ in vitro were ranging from 75 ppm to 100 ppm, whereas the optimal dose of COM suggested for 500 ppm.
Interaction Of Platelet Activating Factor Acetyl Hydrolase (Paf Ah) Enzyme In Gln281 To Arg281 Mutation Toward Paf And Its Molecular Dynamic Jayarani Fatimah Putri; widodo .; Muhammad Saifur Rohman
Journal of Tropical Life Science Vol. 4 No. 1 (2014)
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Abstract

Platelet Activating Factor Acetyl Hydrolase (PAF AH) or LpPLA2 is key enzyme in myocardial infarction catalyzes the sn-2 acetyl group of Platelet Activating Factor (PAF) into lyso PAF and acetate as non-potent inflammatory molecules. PAF AH plays a critical role in arterial plaque development of Coronary Artery Disease (CAD). A crystal structure of PAF AH complexes with other ligand and effects of amino acid alteration to protein plasma consequence have also been reported. Here we report on the result of molecular docking and Molecular dynamic (MD) simulation carried out for PAF AH wild type (WT)/PAF and mutant Q281R/PAF complexes. Docking result shown that amino acid residues on active site of Q281 PAF AH mutant have not recognized on PAF AH. Eelectrostatics and hydrophobic bonds significantly reduced in Q281R than wild type. In the 7500 ps MD simulation Q281R showed less dynamics than WT but enzymatic machinary of mutant Q281R was not interrupted during MD simulation as well as PAF AH wildtype. These findings clearly indicated the importance effect of mutant Q281R in PAF AH recognition to its substrate
Wild Musa Species Collection of Purwodadi Botanic Garden: Inventory and Its Morpho-taxonomic Review Lia Hapsari
Journal of Tropical Life Science Vol. 4 No. 1 (2014)
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Abstract

Indonesia, being part of the center of origin of bananas (Musaceae), has a large number diversity of bananas both wild seeded species and edible seedless cultivated varieties. Inventory of wild Musa species in Purwodadi Botanic Garden has been conducted through compiling data records from PBGs Registration section, field inspection and observation to living collections in the garden, herbarium specimens and literature studies. The results show that total 17 wild Musa accessions has been recorded planted in Purwodadi Botanic Garden since 1990 until 2012; comprises of 8 Musa acuminata sub species, 2 Musa balbisiana forms, 1 Musa ornata, 1 Musa troglodytarum, 1 Musa borneensis and 4 unidentified species Musa spp.; but only 8 living accessions remained in 2012. Morpho-taxonomic review of those 8 wild Musa accessions remained will be discussed in this paper including their geographical distributions. According to its differentiated morphological characteristics observations, it is known that there are three accessions were resembled cultivars and one unidentified species have been determined its species level, so that their registration identity needs to be revised. It is important next to prioritize ex-situ conservation of wild Musa species not yet collected in Purwodadi Botanic Garden especially from Eastern Indonesia.
Excess Fibrin Deposits Decrease Fetal Weight of Pregnant Mice Infected by Plasmodium berghei Desy Andari; Zainabur Rahma; Yuliyanik yuliyanik; Sujarot Dwi Sasmito; Karyono Mintaroem; Loeki Enggar Fitri
Journal of Tropical Life Science Vol. 4 No. 2 (2014)
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Abstract

Low birth weight is commonly attributed to malaria in pregnancy, but the cellular and molecular mechanisms that underlie this poor birth outcome are incompletely understood. A universally described histopathological feature of placental malaria is excessive deposition of fibrin, the end-product of the coagulation cascade. This study was conducted to compare fibrin deposit in pregnant mice that infected by Plasmodium berghei (treatment group) to the normal pregnant mice (control group) and its association with fetal weight. This research is in vivo experimental laboratory study that used 18 pregnant Balb/c mice which divided to the control the group (8 mice) and treatment group (9 mice infected by P.berghei). Placentas were staining with Haematoxylin-Eosin (HE) for fibrin deposits examination whereas fetal weight was performed with Mettler analytical balance AE 50. Fetal weight of the treatment group was lower than those of the control group (t test, p=0,002). Fibrin deposits were increased in the treatment group (t test, p=0,005) and influenced weight of fetuses (Spearman r=-0,586, p=0,014). Weights of fetuses are interfered by fibrin deposits during malaria infection.
The Correlation between Serum Concentration of Vitamin D with Vitamin D Receptor Expression and Disease Activity in Indonesian Patients with Systemic Lupus Erythematosus: Preliminary Study Kusworini Handono; Laksmi Karunia Tanuwijaya; Loeki Enggar Fitri; Handono Kalim
Journal of Tropical Life Science Vol. 4 No. 2 (2014)
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Abstract

The vitamin D role on the immune response of systemic lupus erythematosus (SLE) patient is mediated by vitamin D receptor (VDR). Low level of vitamin D correlated with disease activity in SLE patients, and circulating levels of activated vitamin D (1,25(OH)2D) contribute to VDR protein levels and its function. The objective of this study was to determine the correlation between vitamin D status with expression of VDR in peripheral blood mononuclear cell (PBMC) and the disease activity in SLE patients. The Research Subjects were 15 SLE patients (ACR 1997 criteria) from the Rheumato-Immunology Division, dr. Saiful Anwar Hospital, Malang and 5 healthy controls. Serum vitamin D (25(OH)D3) level was assessed using ELISA method. VDR expression in PBMC was assessed using immunocytochemistry technique. The disease activity was measured by Systemic Lupus Erythematosus Disease Activity Index (SLEDAI) score. This study showed no difference on VDR expression in PBMC between patient and healthy control group, but patient with vitamin D deficiency had lower VDR expression in PBMC than the other group. No difference on SLEDAI score between the group. Vitamin D status correlated positively with VDR expression in PBMC (p < 0,035, r = 0,473). However vitamin D status did not correlate with disease activity scores (p = 0,686).
Organic Agroedu-Tourism Attractions Toward An Interesting Outdoor Environmental Education To Junior High School Students Ayu Raisa Khairun Nisa'; Setijono Samino; Endang Arisoesilaningsih
Journal of Tropical Life Science Vol. 4 No. 2 (2014)
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Abstract

The purpose of this study is to evaluate two organic agroedu-tourism attractions developed to 42 students of eight graders of three junior high schools in the village and one school in the city. Organic agroedu-tourism offers two tour programs namely Organic Garden Tour and Organic Farming Technology. Appreciations of the participants were determined by pre and post test after joining the organic agroedu-tourism. The data were analyzed by using descriptive statistics. This study shows that 78.6 % of the participants prefer the Organic Garden Tour and 21.4% find that the Organic Farming Technology is better. Organic agroedu-tourism can improve cognitive aspect of participants from score 1 turned into 3. Most participants had a high interest in joining the organic egroedu-tourism. Organic agroedu-tourism is also able to meet the expectations of participants that are indicated by an increase in the skills and experience of participants from score 2 to 5. Satisfaction of the participants can also be assessed from the high appreciation average before and after joining Organic agroedu-tourism, which are score 4. The appreciation of the participant is shown by their willingness to invite other people to join. Factors affecting participants satisfaction were family background, socio-economy background, personal perception and knowledge. Based on the result, organic agroedu-tourism shows strength to develop the participants understanding, knowledge and skills in integrated organic farming system, even though not all participants are interested to join all programs provided. Farmer groups need to improve the quality of organic agroedu-tourism, for example, completing the facilities and agreed on tourism attractions, enhancing farmers skills as tour guide, providing organic agroedu-tourism program relevant to the school curriculum, and promoting it to public.
Morphological Variation of Six Pigmented Rice Local Varieties Grown in Organic Rice Field at Sengguruh Village, Kepanjen Subdistrict, Malang District Shinta Shinta; Serafinah Indriyani; Endang Arisoesilaningsih
Journal of Tropical Life Science Vol. 4 No. 2 (2014)
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Abstract

Indonesia is the third richest country for pigmented rice source. Morphological character of pigmented rice in Indonesia is less reported since recent publications and descriptions of pigmented rice are only limited to black rice of Cempo Ireng variety and red rice of Aek Sibundong variety. Pigmented rice varieties in Indonesia are cultivated in an organic way. These varieties are Wojalaka black rice of East Nusa Tenggara (NTT), Manggarai of NTT, Toraja of South Sulawesi, Cempo Ireng of Central Java and red rice of Aek Sibundong (leading variety) and Baubau of Southeast Sulawesi. The objective of research was to compare the morphological variation of root, stem, leaf, panicle, floret and the colour of milk mature grain and mature grain by observing the vegetative and generative parts of six local rice varieties. Research had been conducted from February 2012 to February 2014 in Sengguruh Village, Kepanjen District, Malang Regency. This study type was quasi-experiment with eleven replications. Group Random Design was used. The observation was given upon vegetative, reproductive and maturity phases as groups. Independent variables in this research were six rice varieties, while the dependent variable was morphological variation (root, stem, leaf, panicle, floret, milk mature grain and mature grain). The data of morphological variation were tabulated through MS Excel 2007 for windows. Descriptive data analysis was used to determine the average rate and the deviation standard of each variable. Data were analyzed by ANOVA at a cut off value of &alpha; of 0.05. It was followed by HSD Tukey Test facilitated by SPSS 16.0. The analysis of multivariate data in cluster and biplot was carried out with PAST. The result of the study indicated that there was morphological difference on stem, leaf, panicle, floret, milk mature grain and mature grain. The colour of the stem in Aek Sibundong variety was purple, while that of other varieties was green. Toraja and Manggarai varieties had the highest height with 163-168 cm, followed by Cempo Ireng with 139 cm, Wojalaka and Baubau with 110-112 cm. Aek Sibundong Variety had the lowest height with 99 cm. Aek Sibundong and Wojalaka varieties had 6-7 internodes which were the greatest number of internode, while other varieties only had 4-5 internodes. Some varieties, such as Aek Sibundong, Wojalaka and Baubau had short and small leaf. The leaflet angle of Aek Sibundong and Baubau were 140 and it might be said as upright, while that of Wojalaka was 430 or moderate. Cempo Ireng, Manggarai and Toraja had its 63-880 of the leaflet angle or it was said as flat. Cempo Ireng and Wojalaka were included into Rice Indica Group while Toraja, Manggarai, Cempo Ireng and Baubau belonged to Rice Javanica Group. There were two morphological groups. Group 1 included Toraja, Manggarai and Cempo Ireng, while Group 2 consisted of Aek Sibundong, Baubau and Wojalaka.

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