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Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences
Published by Universitas Brawijaya
ISSN : 20875517     EISSN : 25274376     DOI : -
Core Subject : Agriculture, Social,
Agriculture, Biological Sciences & Forestry Environmental Science
The Journal of Tropical Life Science (JTLS) provides publication of full-length papers, short communication and review articles describing of new finding or theory in living system, cells and molecular level in tropical life science and related areas. The journal publishes articles that report novel findings of wide Tropical Life system phenomenon in the areas of biodiversity, agriculture, fisheries, health, husbandry, forestry and environmental technology. JTLS has 1 volume with 3 issues per year.
Arjuna Subject : -
Articles 1,001 Documents
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CD4+CD25+ T cells maintain homeostasis by promoting TER-119 cell development and inhibiting T cell activation Muhaimin Rifai
Journal of Tropical Life Science Vol. 4 No. 1 (2014)
Publisher : Journal of Tropical Life Science

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Abstract

CD4+CD25+ regulatory T cells involved in the regulation of self-tolerance and normality of homeostasis. CD122 deficient mice are model animals that have an abnormal immune system characteristically have a high number of activated T cells and TER-119 cell decreased. Here we showed evidence that the transfer of CD4+CD25+ regulatory T cells derived from normal mice to CD122-defficient neonates prevent the development of activated memory T cells and elicit TER-119 differentiation. Bone marrow reconstitution derived from CD122-/- mice to normal mice resulting tolerance to individual that genetically different. Importantly, CD4+CD25+ regulatory T cells derived from normal mice can replace CD4+CD25+ cells derived from CD122-/- mice. The results of this experiment suggest that regulatory T cells from normal mice exert a critical role in maintaining peripheral tolerance and controlling hematopoietic disorder.
Morphological And Biochemical Responses Of Saccharum Spontaneum L. Accessions To Drought Stress Aminatun Munawarti; Taryono Taryono; Endang Semiarti; Sismindari Sismindari
Journal of Tropical Life Science Vol. 4 No. 1 (2014)
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Abstract

Glagah (Saccharum spontaneum L.) has potential value as a crop species and may also be used in sugarcane breeding programs; however, this germplasm has not been extensively used in breeding programs, primarily in relation to improve drought tolerance. The objectives of this experiment were to evaluate the effect of drought stress initiated at vegetative growth stage on growth, leaf proline content and protein pattern of seven glagah accessions (BOT-53, BOT-54, BOT-60, BOT-77, BOT-78, BOT-84, and BOT-88). The plants were propagated from single node stalk segments in polybag in the field under non-stress condition for two months. The two month-old plants were then subjected to drought stress by withholding watering for eight weeks. Untreated control plants were watered every two days. Results indicated that drought stress reduced plant height, stalk diameter and green leaf number. On the other hand, there was a little difference between drought-stressed and control plants in terms of proline content. The protein pattern showed that drought stress caused a change in gene expression in the form of induction or repression of protein expression. A specific protein with a low range of molecular weight (Rf value about 0.647) showed constitutively expressed in accession BOT-53 but drought-inducible expressed in BOT-54. Keywords: Drought stress, glagah, proline, protein pattern, Saccharum spontaneum
Ecotourism and Climates changes: the ecolodge contribution in global warming mitigation Luchman Hakim; Nobukazu Nakagoshi
Journal of Tropical Life Science Vol. 4 No. 1 (2014)
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Abstract

Global attention to the global warming reduction has invite numerous strategy implemented with the objectives is mitigating greenhouse gasses emission which threats to the future of living in biosphere. Essentially, absorbing CO2 from atmosphere and sequestering in terrestrial ecosystem is one of the significant strategy. While in developing countries it is become essential, support for forest conservation, afforestation and effort to increase terrestrial ability to capture and storage carbon is poor. Ecotourism offer potential key to solved such problems by promoting ecolodge as a sustainable tourism accomodations. This paper aims to explore the potential of ecotourism sector to alleviate global warming and establishing framework for ecolodge planning and development in tropical developing countries. This paper highlight the significant of ecolodge attraction and development management to meet proper carbon capture and sequestration mechanism. The attraction management and developing programs ultimately able to increase plants biomass while accommodation able to practicing energy efficient and optimizing reuse and recycle approach. It will become the potential solution for reducing greenhouse gas emissions and create clean development strategy.
Evaluation of Antioxidant Activity of Some Selected Tropical Fruits in South Kalimantan, Indonesia Efrilia Tanjung; Muhammad Hafidz MS; Iskandar Thalib; Eko Suhartono
Journal of Tropical Life Science Vol. 4 No. 3 (2014)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.04.03.09

Abstract

In the present study antioxidant and antioxidant activity of some tropical fruits was evaluated. Antioxidants are compounds or molecules that can scavenging and prevent free radicals and reactive oxygen species that can caused a cell damage. Fruit was known as a source of antioxidant. South Kalimantan Indonesia, has a variety of fruit such as mentega, nangka, timun suri and kuranji. Study for evaluating the antioxidant levels and activity of those fruit were never been investigated. Thus, our study aim to measure the antioxidant levels and antioxidant activtity of those selected fruits. Ascorbic acid, lycopene, b-carotene levels and antioxidant activtity of four selected tropical fruits was evaluated using spectrophotometer. The result of this studied suggest that the four selected tropical fruits is potential antioxidant because it contained ascorbic acid, b-carotene, lycopene and had effect of scavenging radical hydroxyl, hydrogen peroxide and chellating ferrous iron.
The Correlation Between IL-1β and IL-10 Levels in Estimating The Risk of Febrile Seizures Andi Gunawan; M Muid; Hidayat Suyudi; Barlianto wisnu; Krisni Subandiyah
Journal of Tropical Life Science Vol. 4 No. 2 (2014)
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Abstract

Febrile seizures are the most common forms of seizures in children with fever. A data from clinical and experimental studies suggest the potential role of immune generate products in their genesis. The balance between proinflammatory (IL-1β) and anti-inflammatory (IL-10) cytokines influences the regulation of infections and plays a role in the pathogenesis of febrile seizures. In existing literature, there is no research on the correlation between IL-1β and IL-10 levels as well as the ratio of IL-1β to IL-10 in estimating the risk of febrile seizures in seizure prone children aged between 3 months and 5 years. In this study, IL-1β, IL-10 levels and ratio of IL-1β to IL-10 in the risk of febrile seizures were investigated, and respondents were divided into three groups: febrile seizures, febrile without seizures and healthy children without histories of febrile seizure. This study has used A cross-sectional design, and each group had 17 co-respondents. IL-1β and IL-10 levels were measured by ELISA. Data were analyzed by SPSS 15. Findings showed there were significant differences between IL-1β and IL-10 as well as in the ratio of IL-1β to IL-10 between febrile seizure patients and healthy children. It was concluded that there were significant correlations between IL-1β and IL-10 levels as well as IL-1β to IL-10 ratios in estimating the risk of febrile seizures.
Pharmacognostic Investigation of Clerodendrum phlomidis Linn. f. Root Dinesh Kumar; Ajay Kumar; Om Prakash
Journal of Tropical Life Science Vol. 4 No. 2 (2014)
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Abstract

The present study was aimed to perform the pharmacognostic evaluation of Clerodendrum phlomidis Linn. f. root in terms of organoleptic, macro-microscopy, fluorescence analysis and physicochemical parameters. The characteristic macroscopic features showed that the root consists of 7-15 cm long, 0.2 -3.0 cm thick pieces which are cylindrical, tough and yellowish-brown externally, with hard fracture and slightly astringent taste. The main microscopic characters of the root shows exfoliating cork, having 10-15 rows of tangentially elongated, thick-walled cells. Cortex consists of round to oval parenchymatous cells, a few containing rhomboidal shaped calcium oxalate crystals. Endodermis consists of 3-4 layers of non-lignified, thick-walled rounded parenchymatous cells followed by a single pericyclic layer. Phloem consists of isodiametric, thin-walled, parenchymatous cells whereas xylem contains lignified pitted vessels. Medullary rays consisting of biseriate layer of lignified and radially elongated parenchymatous cells, is narrower in the xylem region while wider in the phloem region. Further, physicochemical analysis of the root power reports total ash, water soluble ash, sulphated ash as 7.8, 0.9 and 10.3 % w/w respectively. Successive extraction of the root powder with petroleum ether, chloroform, alcohol, water yielded 2.2, 2.4, 12.4 and 9.6 % w/w extracts respectively. Fluorescence study imparted characteristic colours to the root powder when observed under visible, short and long wavelength light. Various pharmacognostic parameters evaluated in this study helps in botanical identification and standardization of Clerodendrum phlomidis L. root part in crude form and provide the authentic data for the researchers and scientists involved in carrying out further research on this plant part.
Selecting Tree Species with High Carbon Stock Potency from Tropical Upland Forest of Bedugul-Bali, Indonesia Arief Priyadi; Sutomo Sutomo; I Dewa Putu Darma; Ida Bagus Ketut Arinasa
Journal of Tropical Life Science Vol. 4 No. 3 (2014)
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Abstract

Vegetation studies to reveal tree diversity and its contribution to carbon stock were conducted in three differ­ent sites of upland forest in Bali, Indonesia. The sites were located approximately 60 km north of the Bali Province capital city of Denpasar in an area named Bedugul. Those three sites were Mt. Mangu (forest area east of Beratan lake), forest area west of Buyan lake and forest area south of Tamblingan lake. There were 44, 29, and 21 tree species of 14, 19, 14 families with Shannon Diversity Index (H) of 2.87, 2.64 and 1.69 respectively. Carbon stock average of above ground tree biomass from these sites were 214.2, 693.0 and 749.1 ton.ha-1 respectively. Tree species with top Summed Dominance Ratio (SDR) in each of those sites were Platea latifolia in Mt. Mangu, Plan­chonella sp. in Buyan, and Tabernaemontana macrocarpa in Tamblingan. Average carbon content of these three species were 493.25, 12,876.26 and 40.35 kg.ha-1 respectively.
Bovine And Human Zona Pellucida 3 Gene Glycans Site Prediction Using In Silico Analysis Nurul Jadid Mubarakati; Aulanni'am Aulanni'am; Sutiman Bambang Sumitro; Gatot Ciptadi
Journal of Tropical Life Science Vol. 4 No. 3 (2014)
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Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.04.03.08

Abstract

Zona pellucida is one of the protective layer of the egg cell and has a function as an intermediary species-specific fertilization. Glycoproteins of human and bovine zona pellucida is composed of three types, namely ZP1, ZP2 and ZP3. ZP3 gene has amino acid sequence homology with other mammals. Oligosaccharides components of the zona pellucida glycans are composed from units of asparagine residues (N -linked) and serine/threonine (O -linked). The aims of this study was to analyze the DNA sequences of human and bovine and further predicts glycans site on amino acid sequence of human and bovine ZP3. In this study, ZP3 gene fragments have been isolated from bovine and humans were analyzed in Silico. This work were conducted by comparing the data of DNA sequence from human and bovine PCR product using NCBI BLAST. The results showed that there were similarities at amino acid positions number 23-38. bZP3 sequence had three glycans site (Asn-X-Thr/Ser) and one site on hZP3 glycans. One of the sites was conserved between the two species.
37% Phosphoric Acid Induced Stronger Matrix Metalloproteinase-8 Expression of the Dental Pulp than 19% Ethylene Diamine Tetraacetic Acid Nadie Fatimatuzzahro; Tetiana Haniastuti; Juni Handajani
Journal of Tropical Life Science Vol. 4 No. 3 (2014)
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Abstract

Etching agents such as ethylene diamine tetraacetic acid (EDTA) and phosphoric acid which are widely used in adhesive restoration system aimed to increase for retention of restorative materials, may act a chemical irritant that induce inflammation of dental pulp. Inflammation is a body response against irritant and infectious agents. Matrix metalloproteinase-8, the major collagenolytic enzyme, degrades collagen type 1. This enzyme is expressed in low level in normal condition, however, the expression will increase during inflammation. The purpose of the present research was to study the effect of 19% EDTA and 37% phosphoric acid application as an etching agents on the MMP-8 expression of dental pulp. Forty-five male Sprague Dawley rats were divided into 3 groups. Cavity preparation was made on the occlusal surface of maxillary first molar using a round diamond bur. 19% EDTA, 37% phosphoric acid, and distilled water were applied on the surface of the cavity of the teeth in group I, II, and III subsequently. The cavity then filed by glass ionomer cements. The rats were sacrified at 1, 3, 5, 7, and 14 days after the treatment (n=3 for each day). The specimens were then processed histologically. Immunohistochemical (IHC) analysis was performed using rabbit anti rat MMP-8 polyclonal antibody to examine MMP-8 expression and HE (Hematoxylen Eosin) staining to observe the number of macrophages. The results showed 37% phos­phoric acid application induced stronger expression of MMP-8 and higher number of macrophages than 19% EDTA. The strongest expression of MMP-8 seems on 5 days after the treatment where the highest number of macrophages were also found.
Genistein Increase Intracellular Distribution of the High Motility Group Box-1 through p38 Pathway in HeLa culture cells induced by Tumor Necrosis Factor-α Merlita Herbani; Aris Widodo; Hidayat Suyuti
Journal of Tropical Life Science Vol. 4 No. 2 (2014)
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Abstract

Cervical cancer is one kind of many cancer that cause death to women around the world. Many studies had support the statement that inflammation has a strong linkage with cancer development. Several factors like proinflammatory factor can influence tumor cell microenvironment, and induce a faster proliferation. TNF-α is suspected can induce proliferation. While cancer itself can induce inflammation, which is marked by several marker. One of them is HMGB1, released from the cell as active secretory lysosomes or passive diffusion. Genistein has demonstrated growth inhibitory effects of various types of cancer cells. It inhibits tyrosine kinase pathway, which can be activated by TNF-α. One of those pathways that have the link with proliferation is p38. This study tries to reveal about inhibitory effect of genistein toward p38 pathway that had been activated by TNF-α. This research was conducted by exposing cultured HeLa cells with various doses of genistein for 90 minutes, and then exposed to TNF - α 10 ng / mL for 20 minutes. Observations were made with a confocal microscope, by staining the cells with pp38-TRITC and HMGB1 antibody. The intensity was measured and analyzed by Fluoview software. The results suggest that there be significant differences between pp38 intranuclear intensity and HMGB1 extranuclear intensity of each dose of genistein (p = 0.000, ANOVA). pp38 and HMGB1 intensity were increased along with increasing genistein dose, but at high dose there were noted decreasing of pp38 and HMGB1 intensity. At apoptotic dose, pp38 and HMGB1 intensity were increased markedly, showing the effect of apoptosis. In general, increasing doses of genistein increase intranuclear p38 activation and HMGB1 extranuclear translocation. So there were a strong linkage between p38 activation and HMGB1 translocation in this study.

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