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Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences
Published by Universitas Brawijaya
ISSN : 20875517     EISSN : 25274376     DOI : -
Core Subject : Agriculture, Social,
Agriculture, Biological Sciences & Forestry Environmental Science
The Journal of Tropical Life Science (JTLS) provides publication of full-length papers, short communication and review articles describing of new finding or theory in living system, cells and molecular level in tropical life science and related areas. The journal publishes articles that report novel findings of wide Tropical Life system phenomenon in the areas of biodiversity, agriculture, fisheries, health, husbandry, forestry and environmental technology. JTLS has 1 volume with 3 issues per year.
Arjuna Subject : -
Articles 986 Documents
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Sequence Analysis of Rifampicin Resistance Determining Region (RRDR) of Mycobacterium tuberculosis Shrestha, Mitesh; Chaudhary, Hemanta Kumari; Chaudhary, Pradip Kumar; Poudel, Bal Hari
Journal of Tropical Life Science Vol 8, No 1 (2018)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (915.368 KB) | DOI: 10.11594/jtls.08.01.15

Abstract

Mycobacterium tuberculosis has become the cause for one of the most dreadful disease which the mankind has ever known i.e. Tuberculosis. The organism holds the ability to infect multiple organs at a time resulting in multiple symptomatic presentations in pathogenic condition while in non-pathogenic condition, it can lay dormant and remain asymptomatic. The research work presented here aimed at sequencing of Rifampicin Resistance Determining Region (RRDR) of the rpoB gene present in phenotypically multidrug resistant M. tuberculosis. The findings showed that the major point of mutations to be present within this region was at codon 516, 526, and 531. Early diagnosis of multidrug resistance in any pathogen has become a pre – requisite for proper treatment and efficient elimination of pathogenic organisms from the host with minimal toxicity. Similarly, understanding the mutation dynamics of target genes also help in novel drug design and discovery.
Allelopathy Potential of Alpinia malaccensis (Burm. F.) Roxb. due to Seeds Germination and Growth of Merremia peltata (L.) Merril Aisah, Siti; Sulistijorini, Sulistijorini; Setyawati, Titiek
Journal of Tropical Life Science Vol 8, No 2 (2018)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (414.447 KB) | DOI: 10.11594/jtls.08.02.05

Abstract

Allelopathy is a natural strategy for the protection or inhibition toward other vegetation through the release of chemicals into the environment. Alpinia malaccensis is thought to be capable of carrying out allelopathic mechanisms, as this species is found to grow well on land invaded by Merremia peltata. Invasive type control with alleopathic mechanism is an alternative to consider as it does not leave a potential residue as a contaminant of soil as it is chemically controlled. The study attempt to investigate the content of A. malaccensis allelochemicals and analyzed the alelopathy potential of A. malaccensis on seed germination and seedling growth of M. peltata. Research method used completely randomized design with 6 replicates for seed treatment and three replicates for seedling treatment. Seeds which have relatively similar size and weight were obtained from the field. Seeds were germinated in petri dishes that have been coated by filter paper, each petri dish contained 6 seeds of M. peltata. The treatment of the seeds germination was performed by giving 3 ml of rhizomes and leaves extracts of A. malaccensis (control, 30 g/L, 60 g/L, 90 g/L, 120 g/L, and 150 g/L). The treatment of the seedlings was performed by giving 30 ml of rhizomes and leaves extracts of A. malaccensis (control, 50 g/L, 100 g/L, 150 g/L, and 200 g/L). The analysis of chemical compounds of fresh rhizomes and leaves showed that A. malaccensis contains alcohol, amide, fatty acid, phenol, ketones, and terpenoids. Rhizomes and leaves extracts 150 g/L of A. malaccensis showed the highest inhibition in germination, dry weight, plumule and radicle length of M. peltata sprout parameters. Rhizomes and leaves extract 200 g/L of A. malaccensis showed the highest inhibition in tendril length, amount of leaves, length and width of M. peltata leaves parameters. To be more efficient, application in the field should use low concentration of extracts that can inhibit the M. peltata. Results of this study are expected to provide information about alternative solutions to suppress the invasion of M. peltata to preserve ecosystems of Bukit Barisan Selatan National Park (BBSNP) in Lampung.
Phytochemical and Antimicrobial Screening of Native Plant Swertia chirayita (Roxb. ex Fleming) Karst from Rasuwa district of Nepal Subedi, Ishwar; Karki, Tika Bahadur
Journal of Tropical Life Science Vol 8, No 2 (2018)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (315.431 KB) | DOI: 10.11594/jtls.08.02.10

Abstract

Swertia chirayita is one of the highly traded medicinal plants of Nepal. This plant was chosen for investigation as folk medicines and then collected from the diverse habitats of Rasuwa district of Nepal and specimens have been checked and identified at National Herbarium and Plant Laboratories (NHPL), Godavari, Lalitpur. The identified plant was subjected to study physiochemical and biological activity. The stem, root and leaves of the plant were dried, made powder and mixed at the ratio of 1 : 1 : 1 and then extracted using methanol solvent by cold and warm method. The percentage yield from the plant was highest in warm methanol with 3.73%, followed by cold methanol with 2.28%. Plant extract showed the presence of phytochemicals like basic alka-loids, coumarin, glycosides, steroids, quinones, flavonoid and terpenoids. The antibacterial activity of the ex-tract showed significant bioactive by inhibiting the growth of selected pathogenic microbial species for the test. The zone of inhibition (ZOI) shown by the extracts was comparable to the standard antibiotics. Similar-ly, proximate composition was also carried out. The antioxidant activity of the sample was found to be 62.54% at 500 µg/mL and 15.32% at 100 µg/mL by radical scavenging method and showed significant antioxi-dants potential. 
α-SMA Expression Increased Over Cell Passages and Decreased by Exogenous TGF-β1, In Vitro Studies on Myofibroblast Derived from Orbital Socket Contracture Shintiya Dewi, Debby; Chairinnisa, Elsa Safira; Sujuti, Hidayat; Lirawati, Diana; Hernowati, Tinny Endang
Journal of Tropical Life Science Vol 8, No 2 (2018)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.08.02.15

Abstract

α-smooth muscle actin (α-SMA), a marker of myofibroblast, induces cytoskeleton reorganization, increases contractility and stimulates cell migration in TGF-β1 induced stress fibers. The aims of the present study were to determine the level of α-SMA expression and morphological cell changes in different passages of myofi-broblasts with varied TGF-β1 concentrations. Myofibroblast cell cultures were derived from fibrotic tissues of fourth degree socket contracture. The α-SMA expression level was measured in myofibroblast cultures pas-sage I, II, and III with and without 10 ng/mL TGF-β1, and in passage III with 2.5; 5; 10; and 20 ng/mL TGF-β1. Results: The levels of α-SMA expression level in passage I to III were I 31.42 ± 3.4; 40.34 ± 8.14 and 56.37 ± 7.57, respectively. Addition of 10 ng/mL TGF-β1 into passage I-III myofibroblast cultures resulted in α-SMA expression level of 31.24 ± 2.93; 36.81 ± 6.09; and 14.29 ± 2.72, respectively. Myoblasts passage III showed the lowest α-SMA expression level following exposure to TGF-β1 10 ng/mL (22.37 ± 12.86) and highest without TGF-β1 (48.34 ± 13.36), however no morphological changes detected. α-SMA expression level increased with cell passages, decreases with addition of TGF-β1 while not affecting morphology of myofibroblast derived from the orbital socket contracture.
ASSESSMENT OF RIPARIAN VEGETATION PATTERN ALONG ALISTA HILLY RIVER BASIN Parmawati, Rita; Rokani, Lutvita Erya; Yakub, Arham; Riarmanto, Arief Kurnia; Pertiwi, Maya; Hidayat, Herman; Kurnianto, Agung Sih
Journal of Tropical Life Science Vol 8, No 3 (2018)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.08.03.05

Abstract

Riparians are undergoing extensive reductions and changes in their natural composition into agricultural and urban area. The relationship between the vegetation distribution pattern and the riparian topography factor has been assumed to typically represent a biome-specific or vegetation-specific constant. This study aim is to analyze the vegetation distribution pattern with topography along Alista river in Dau District, Malang. We surveyed 3 stations that represent a zone of edge gradient between the river and the mainland: levee, mid-flood, and slough. We used the wetland classification (Obligate - OBL; Facultative Wetland-FACW; Facultative Upland (FACU); and Upland (UPL) for each species found from various sources. we observed that facultative upland plants have a broad dominance. Mid-floodplain and slough dominated by typical upland species. Although Levee widely consisted of by upland plants, this area has one distinctive obligate species: Equisetum. The presence of OBL species in the levee and mid-floodplain indicate the area is receiving more frequent floods than other areas.
Antimicrobial Production by an Actinomycetes Isolated from The Termite Nest Krishanti, Ni Putu Ratna Ayu; Zulfina, Deni; Wikantyoso, Bramantyo; Zulfitri, Arpiwi; Yusuf, Sulaeman
Journal of Tropical Life Science Vol 8, No 3 (2018)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (497.087 KB)

Abstract

Actinomycetes are Gram positive bacteria that have been detected in diverse ecological niches. Their member species are known to be a main source of various bioactive compounds. The discovery of Actinomycetes from diverse and unexplored resources has also been linked to increased opportunities to obtain novel bioactive compounds. Insect nest material is being investigated as a new source of novel antimicrobial producing Actinomycetes, which could be harnessed for therapeutic potential. A total of 10 Actinomycetes isolates were collected from the nest of Nasutitermes sp. in Pananjung Pangandaran Nature Reserve. These isolates were evaluated for antimicrobial activity against the challenge bacteria (Eschericia coli, Staphylococcus aureus, Bacillus subtilis, Serratia Marcescens) and fungi (Fomitopsis palustris, Fusarium oxysporum, Trichoderma viridae) by dual culture method. The result revealed that several isolate were active against fungi and bacteria. Isolate Pn-TN2 showed the highest level of antibacterial inhibition and the highest antifungal inhibition with Inhibition Rate value more than 80%. By morphological and 16S rRNA gene sequence analysis strongly suggested that the isolate Pn-TN2 belonged to Streptomyces prasinopilosus. We suggested that termite nest is a potential source of bioactive strains of cultivable Actinomycetes for future biotechnological needs.
Linking Zoopharmacognocy with Ethnomedication, An Evidence Base from Sebangau National Park, Central Kalimantan Indonesia Panda, Adventus; Gunawan, Yohanes Edy
Journal of Tropical Life Science Vol 8, No 3 (2018)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.08.03.15

Abstract

The ability of animal to perform self-medication has been studied decades, as well as their relationship with medication practices by communities. Long-term observation of Orangutans' behaviour (Pongo pygmaeus wurmbii), by communities surround Sebangau National Park, has suggested as their modes to today ethno-medication practice. The study was aimed to study the relationship of Orangutans self-medicate behavior with ethno-medication practice, in Sebangau area. The research was conducted in Punggualas, SNP, from 29 April to 03 October 2017. A number of 13 traditional healers (TH) from Karuing (n = 4), Baun Bango (n = 4), and Jahanjang (n = 5) have been interviewed. All plants are listed and photograph. Plants parts, and their mechanism of utilization were also kept for record. Meanwhile, the behavior followed the Orangutan protocol, with focus on their feeding behavior. All data were analyzed descriptively, while the relationship was analyzed using chi-square and F test. We have recorded a total 131 plants at various life forms, in Baun Bango (n = 59), Jahanjang (n = 41), and Karuing (n = 21). Plants that were found similar among three villages, removed, were only 95 left. We observed one female, showing the ability to perform self-mediaction. It is characterized by selectively choosing young leaves of Mezzetia sp., pulp of the Dyera lowii and Ilex cymosa, and lastly chew the entire leaves of Belang Handipek. It suggests a form of prevention against fatigue conditions, and the combination of these three plants species assume to be relating to fitness. This study shows that there is a relationship between the plant part used by the traditional healer and the orang- utan (x2 = 43,887; n = 115, df = 11, p-value = 0.0000), the relationship between the use of plant parts utilized by the traditional healer and orangutans (x2 = 15, 647; n = 50, df = 8, p-value = 0.0000). Furthermore, there is a relationship between the practice of traditional healer treatment using plant parts and Orang-utan (F1, 113 = 230.158; p-value = 0,000). The study urges to isolate secondary metabolites for further investigation, especially in terms of phyto-pharmacy.
Fecal Calprotectin Level of Breast Milk-Formula vs Formula Feeding in Preterm and Low Birth Weight Neonates with Necrotizing Enterocolitis Arisanti, Ditya; Wibowo, Satrio
Journal of Tropical Life Science Vol 9, No 1 (2019)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (141.384 KB)

Abstract

Necrotizing enterocolitis (NE) is inflammatory disease and its prevalence was increase in preterm and low birth weight (LBW) neonates. This study was aimed to investigate the differences of fecal calprotectin level in breast milk-formula vs formula feeding infants in preterm and low birth weight neonates with necrotizing enterocolitis. There are 32 preterm and LBW neonates with NE were divided into two group (breast milk-formula and formula only feeding group). Fecal calprotectin level was measured using ELISA method. This study showed that fecal calprotectin level in breast milk-formula feeding group was insignificantly lower as compared to formula feeding group (independent t-test, p = 0.503). Further analysis showed that fecal calprotectin level was negatively correlated with patient’s outcome (Spearman correlation test, p = 0.03, r = 0.512). We concluded that fecal calprotectin level in breast milk-formula feeding group was insignificantly lower as compared to formula feeding only group in preterm and low birth weight neonates diagnosed with necrotizing enterocolitis. There was negative correlation between fecal calprotectin level and patient’s outcome.
IN VITRO EVALUATION OF FREE RADICAL SCAVENGING, FE2+ AND SNP-INDUCED LIPID PEROXIDATION (RAT BRAIN) ACTIVITIES OF METHANOLIC EXTRACTS FROM THREE (3) NORTHERN NIGERIAN PLANTS LEAF Akinsanmi, Augustina Oduje; Johnson, Omolara Titilayo; Longdet, Ishaya Yohanna; Aguiyi, John Chinyere
Journal of Tropical Life Science Vol 9, No 1 (2019)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.09.01.10

Abstract

Over the years research findings have shown that free radicals are the basis for many diseases. This study sought to investigate the free radical scavenging and inhibition of lipid peroxidation activities of some three Northern Nigerian plant leaf; Boswellia dalzielii Hutch. (Burseraceae) (BDL), Detarium microcarpum Guill. and Perr. (DML), and Daniellia oliveiri (Rolfe) Hutch. & Dalz (DOL). The study investigated both qualitative and quantitative phytochemical screening, free radical scavenging activities of 1, 1-diphenyl?2-picrylhydrazyl DPPH, total antioxidant capacity ABTS, hydroxyl, reducing property and inhibition of lipid peroxidation. All the seven phytochemicals screened were all present in both BDL and DML plant extracts but saponins and glycosides were absent in DOL plant leaf. The result revealed that BDL methanolic extract had the highest total flavonoid and phenolic content while BDL methanolic extract had the highest alkaloid content. DOL methanolic extract had the least content in all the 3 phytochemicals quantified. BDL methanolic extract had the highest free radical scavenging activity in 1, 1-diphenyl?2-picrylhydrazyl (DPPH), Hydroxyl and reducing power assays. While DML methanolic extract, had the highest free radical scavenging ability in 2, 2-azino-bis(3-ethylbenzazoline-6-sulphonic acid (TEAC) assays. The IC50 of BDL extract (12.37 mg/mL) was not significantly different (p > 0.05) from DML extract (10.39 mg/mL) in the Fe2+-induced lipid peroxidation assay. While, the IC50 of DML extract (14.83 mg/mL) was significantly higher (p < 0.05) than the BDL extract (19.92 mg/mL) in the SNP-induced lipid peroxidation. The DOL extract had the least scavenging and peroxidative inhibitory activity in all the assays carried out.  We therefore concluded that the synergistic free radical scavenging activities and inhibition of lipid peroxidation of the three plants studied (especially, BDL and DML), provides a biochemical rationale for their usage as a medicinal plant.
Floristic and Phytoclimatic Study of a Sacred Grove Vegetation of West Midnapore District, West Bengal, India Sen, Uday Kumar; Bhakat, Ram Kumar
Journal of Tropical Life Science Vol 9, No 2 (2019)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.09.02.01

Abstract

Traditional cultural and religious beliefs and practices play a crucial role in the conservation of the environment and biodiversity in the form of the sacred groves. The present study was carried out to explore the plant resources, their biological spectrum, leaf size spectra, the conservation status of a sacred grove, locally known as Narampur Barapir Astana (NBA) in West Midnapore district of West Bengal in India. Floristic list of the study revealed that the vegetation of NBA was diverse and composed of 277 species belonging to 238 genera distributed over 77 families under 36 orders according to APG IV classification. The dominant order and family in terms of species richness were Lamiales 27 (9.75%) and Fabaceae 32 (11.55%) respectively. The biological spectrum shows the study area was classified as “thero-chamae-cryptophytic” type of phytoclimate. As regards the leaf size spectra, mesophyll 56 (20.22%) was found to be high. The study area being a sacred grove is fairly undisturbed. For the long-term conservation of germplasm of the grove some recommendations are suggested.

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