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Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences

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Published by Universitas Brawijaya

ISSN : 20875517 EISSN : 25274376 DOI : -

Core Subject : Agriculture, Social,

Agriculture, Biological Sciences & Forestry Environmental Science

The Journal of Tropical Life Science (JTLS) provides publication of full-length papers, short communication and review articles describing of new finding or theory in living system, cells and molecular level in tropical life science and related areas. The journal publishes articles that report novel findings of wide Tropical Life system phenomenon in the areas of biodiversity, agriculture, fisheries, health, husbandry, forestry and environmental technology. JTLS has 1 volume with 3 issues per year.

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Articles 986 Documents

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The Use of Fish Flour and Palm Sugar Medium to Grow Mataram Indigenous Isolate of Entomopathogenic Bacillus thuringiensis for Controlling Aedes agypti Larvae Suryadi, Bambang Fajar; Hidayat, Hilal Aisyah; Tresnani, Galuh
Journal of Tropical Life Science Vol 9, No 2 (2019)
Publisher : Journal of Tropical Life Science

To grow Mataram indigenous local isolate of entomopathonic Bacillus thuringiensis using 4 combinations of fish flour and palm sugar natural liquid medium. The culture was then tested against 3rd instar Aedes aegypti larvae to gain lethal concentration (LC) value within 72-hour test. Four combinations of fish flour and palm sugar liquid medium (in 1:1; 1:3; 1:5 and 1:7 weight ratio) were made to grow B. thuringiensis isolate Bt-TP2B. Cell, endospore and protein crystal were recorded every 24 hours within 72-hour incubation. Seventy-two-hour-old B. thuringiensis cultures from those media were serially diluted, then tested against 3rd instar B. thuringiensis larvae. Larval death was recorded every 24 hours within 72-hour observation to obtain lethal concentration (LC) values. Results from all medium were compared to those of NYSM standar medium. B. thuringiensis isolate BT-TP2B could grow in fish flour and palm sugar natural medium. The highest toxicity (the lowest LC) was seen on B. thuringiensis grown on fish flour and palm sugar natural medium in 1:1 weight ratio. The LC50 values were 1.98x103 cell/mL (in 24-hour observation) and 1.49x103 cell/mL (in 48-hour and 72-hour observation), while LC90 values were 1.35x103 cell/mL in 24-hour observation and 1.58x103 cell/mL in 48 and 72-hour observations). These values were lower (more toxic) compared to those of NYSM standard medium. Fish flour and palm sugar natural medium could be used to grow entomopathogenic B. thuringiensis. Moreover, this natural medium could increase toxicity of B. thuringiensis against B. thuringiensis3rd instar larvae.Â

Nutritional, Hypoglycemic, and Haematinic Potentiality of Edible Mushroom Pleurotus tuber-regium (Rumph. ex Fr.) Singer Dandapat, Sukumar; Kumar, Manoj; Ranjan, Rakesh; Sinha, Manoranjan Prasad
Journal of Tropical Life Science Vol 9, No 2 (2019)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar

Mycochemical composition, calorific value, the antioxidant activity of Pleurotus tuber- regium was analyzed and impact of P. tuber- regium extract on rat model especially glycemic, vitamins and blood parameters were explored to validate its medicinal importance. Mycochemical screening showed the presence of biochemicals such as phenols, flavonoids, proteins, carbohydrates etc. The extract showed good antioxidant activity (33.62% total antioxidant activity equivalent to 21.30 Âµg ascorbic acid). The extract showed dose -dependent hypoglycaemic activity by significant decreased average blood glucose level at high dose (100.62 Â± 1.04mg/dL) compare to control (124.40 Â± 1.45 mg/dL), haematinic activity by elevation of hemoglobin (14.75 Â± 0.24 g/dL) at a high dose of extract compared to control (11.66 Â± 0.21 g/dL). P. tuber-regium extract elevated vitamin B12 of rats at high dose of extract (449.60 Â± 3.12 pg/mL) compared to control (420.00 Â± 2.86pg/mL). P. tuber-regium extract showed a positive response to hypoglycemic, haematinic, and vitamin level of the body and posses high calorific value. Hence P. tuber-regium can be used as good fodder, medicinal and nutritional supplement.

The Role of Coenzymes on Mercury (Hg2+) Bioremediation by Isolates Pseudomonas aeruginosa KHY2 and Klebsiella pneumonia KHY3 Neneng, Liswara; Gunawan, Yohanes Edy
Journal of Tropical Life Science Vol 8, No 1 (2018)
Publisher : Journal of Tropical Life Science

Mercury pollution is dangerous to health. Previous research was found two potential Gram-negative bacteria for mercury bioremediation, from gold mining in Central Kalimantan, Indonesia.Â These isolates were identified as Pseudomonas aeruginosa KHY2 and Klebsiella pneumonia KHY3. Mechanisms of mercury bioremediation had not known yet by these isolates.Â This study purposed to test the role of coenzymes on mercury bioremediation by these isolate and to determine the coenzymes best level of mercury bioremediation. Experimental design was Completely Randomized Design in a laboratory.Â Treatment factors were coenzymes obtained from vitamins B1, B6, B12, with 6 levels of treatments, included 1 control.Â All treatments were done in Luria Broth media that contain 12 ppm of mercury. Mercury was measured by AAS Shimadzu AA-6200. The results showed that coenzymes effect was very significant to improve mercury bioremediation by P. aeruginosa KHY2 and K. pneumonia KHY3.Â Supplementation of vitamin B12 in culture media, more enhance of mercury bioremediation compared with vitamin B1 and B6. These result above, indicated the mechanism of mercury bioremediation in both isolates, were the enzymatic process.

Malondialdehyde (MDA) Ovary and Estradiol Blood Serum Levels of Premenopause White Rat (Rattus norvegicus) after Turmeric Powder (Curcuma longa L.) Treatment Suprihatin, Teguh; Widyarti, Sri; Rifa'i, Muhaimin; Rahayu, Sri
Journal of Tropical Life Science Vol 9, No 3 (2019)
Publisher : Journal of Tropical Life Science

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Premenopause is a physiological condition in a female individual that has entered the aging period, a condition usually characterized by elevated MDA levels and decreased estrogen levels. The objective of this study was to determine the level of ovarian MDA and estradiol serum levels of premenopausal white rat blood after oral turmeric powder treatment. The animals used were 30 female Wistar strains white rat, age 12 months with an average body weight 200-250 g. The animals were divided into 6 groups, namely the negative control group (P0) with 4 ml/day distilled water treatment; positive control group (P1), this group was treated with pure curcumin powder 6.75 mg/kg BW; treatment group 1 (P2), group was treated with turmeric powder 100 mg/kg BW; treatment group 2 (P3); treatment group 3 (P4); and treatment group 4 (P5), these group were treated with turmeric powder at 200 mg/kg BW; 400 mg/kg BW; and 800 mg/kg BW dose respectively. Oral Treatment was administered daily for 27 days. Blood collection was performed on days 0, 14, and 28. The ovarian collection was conducted on day 28. MDA ovarian level was measured using TBA method and blood serum estradiol level was measured using ELISA method. The results exhibited that the positive control group (P1) and the treatment group (P2-P5) showed significantly lower ovarian MDA levels compared with the negative control group (P0). The turmeric powder dose 200 mg/kg BW (P3) can increase estradiol levels by day 14 (3.32 Â± 0.26 Ïg/mL) and at day 28 (4.01 Â± 0.26 Ïg/mL).

Ethnobotany of MandailingTribe in Batang Gadis National Park Nasution, Aswarina; Chikmawati, Tatik; Walujo, Eko Baroto; Zuhud, Ervizal Amir Muhammad
Journal of Tropical Life Science Vol 8, No 1 (2018)
Publisher : Journal of Tropical Life Science

Â Batang Gadis National Park (BGNP) located in Bukit Barisan Mountains, Sumatera Utara. A Mandailing tribeÂ who lives around the BGNP, has the unique local knowledge, such as processing young stem of rattan (Calamus manan) into pakkat (traditional food) and use rimbang (Solanum torvum) to neutralize toxins. These local knowledge could be lost because it only inherited orally from generation to generation. This study was aimed to reveal ethnobotany knowledge of Mandailing Tribe. The study was conducted in November 2015 in four villages around the BGNP, Sibanggor Jae, Hutabaringin Julu, Pastap Jae, and Botung Villages. Data were collected by interviewing informants in each village as well as the field survey through two approaches, emic and etic. A total of 262 plant species is used by Mandailing Tribe for subsistence and commercial needs. The highest utilization is for foodÂ (106 species), followed by traditional medicines (81 species), firewood (29 species), building materials (35 species), and animal feed (25 species). People also used plant for household appliances, agricultural equipment, art materials, ropes and wrap, and pest control materials. Eme/rice (Oryza sativa) have the highest Index of Cultural Significance (ICS) values. The existence of this species is maintained for its local wisdom. Thus, involvement ofÂ localÂ community will give great contribution to manage and conserve the BGNP area.Â

Glycerol Utilization as Substrate for Ethanol Production in Escherichia coli Recombinant under an Aerobic Condition Pranata, Wahyu Suradi; Suryadarma, Prayoga; Mangunwidjaja, Djumali
Journal of Tropical Life Science Vol 8, No 1 (2018)
Publisher : Journal of Tropical Life Science

The utilization of glycerol as a substrate for ethanol production in E. coli recombinant harboring ethanologenic gens (PDC and ADHB) under aerobic conditions was investigated. This research was conducted by using E. coli BW25113 which compared the growth characteristics on glycerol and glucose as carbon source. E. coli can grow well on both substrates and it consumed glycerol faster than glucose. On glucose, E. coli occurred overflow metabolism that indicated by high acetate accumulation. Meanwhile, on substrate of glycerol the acetate accumulation could be reduced. Using glycerol substrate on E. coli increased piruvate accumulation, comparing with glucose. Therefore, the growth characteristic of glycerol was more effective. In E. coli âˆ†pta/pHfdh/pTadhB-pdc utilized glycerol substrate was able to accumulate pyruvate as intermediate metabolite for produce 2.18 gL-1 ethanol.

Biodiversity of Medicinal Plants at Sambas Botanical Garden, West Kalimantan, Indonesia Sudarmono, Sudarmono
Journal of Tropical Life Science Vol 8, No 2 (2018)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.08.02.04

Botanical garden is one of ex-situ conservation which has function as germ plasm conservation, education/research, and recreation. To conserve plants biodiversity, many local governments in Indonesia propose to cooperate with LIPI to build botanical gardens. Sambas botanical garden with an area Â± 300 ha in West Kalimantan is one of a botanical garden which is under construction. As new exploration site, many biodiversity in this area has not been entirely explored yet including its medicinal plants. Methods used in this study including field survey and interview techniques. The objective of this study is to explore a biodiversity of medicinal plants in that area. This study identified 30 medicinal plants, representing 20 families and 25 genera. It indicated that Sambas botanical garden has many highly potential values of medicinal plants. Therefore, a proper management including medicinal plant for this area is strongly required.Â

The Effect of Human Pellucide Zone 3 Monoclonal Antibody on Expression of Bcl-2 and Bax in Follicle Granulosa Cells of Mice Ovary Natalina, Riny; Nurseta, Tatit; Winarsih, Sri
Journal of Tropical Life Science Vol 8, No 2 (2018)
Publisher : Journal of Tropical Life Science

Pellucide zone 3 (ZP3) involves in fertilization mechanism. Moreover, antibody of ZP3 can develop to inhibit egg and sperm interaction. This study aims to determine the effect of hZP3 (mab-hZP3) monoclonal antibody on the expression of Bcl-2 and Bax in follicle granulosa cells of the mice ovary. Female mice BALB/c were divided into 12 groups which consisted of control and experimental treatment group. Each group was added with 30% of total mice as error sample (1 mice). Each groups were treated differently: 50 Âµl adjuvant Al(OH)3 in 50 Âµl Tris HCl, 20 Âµg Mab-hZP3, 40 Âµg Mab- hZP3, and 60 Âµg Mab-hZP3. Each group was dissected at day 10, 15 and 20. Measurement of Bcl-2 and Bax was performed with immunohistochemistry. Data was analyzed by Two-Way ANOVA. There was no significant effect of Mab-hZP3 administration in various doses on Bcl-2 (p=0.0825), and Bax (p=0.836). There was no significant effect of administration of Mab-hZP3 in time (p=0.807), neither on Bcl-2 expression (p=0.088) and Bax (p=0.227). The lowest Bcl-2 level was found in dose of 60 Âµg in day 15. There was no significant effect of Mab-hZP3 in various doses and time (p=0.691), neither to Bcl-2 and Bax. Such results obtained due to the specificity of a monoclonal antibody that recognizes specific antigen. Mab-hZP3 is proposed as immunocontraception for women causing no disturbance of folliculogenesis.

Home Garden of Local Community in Pancasila Village for Biodiversity Conservation and Ecotourism Sites Development in Tambora Geopark, Sumbawa Island Hakim, Luchman; Kee, Hong Sun
Journal of Tropical Life Science Vol 8, No 2 (2018)
Publisher : Journal of Tropical Life Science

Home gardens of the villages near the conservation area plays an important role, both in ecologicl and socio-economical aspects. The aim of the research is to identify the plant species diversity in home garden in Pancasila Village, Tambora Geopark in Sumbawa Islands. Floristic survey was conducted at 25 home gardens on local community. An in-depth interviews with informant was generated to generate informationÂ related to species value and home garden management. Data was analyzed disruptively. Result of the study shows that 87 plant species (46 families) was grows in home gardens. These plants consist of 49.56 % of Phanerophytes, 8.90% of Chamaephytes, 25.28% of Â Hemicryptophytes and 6.70%of Cryptophytes. According to phytogeograpical origins, 24.41% of the species exclusively are Asian tropic species. These plant has numerous purposes, including staple food, species and vegetables, fruit trees shading trees and ornamental plants. From all species in home garden, some plant has high frequency, including Nephelium lappaceum (f = 0.52), Bougainvillea spectabilis (f = 0.40), Mangifera indicaÂ (f = 0.36), Musa paradisiaca (f = 0.36) and Coffea sp. (f = 0.32). Most of the species was identified as exotic plant species and therefore attention to such species group is needed to protect the environment and biodiversity of Tambora geopark.

Date Fruit Extract (Phoenix dactylifera, Ajwa) Modulates NK Cells and TNF- Alpha in DMBA-Induced Mammary Cancer Sprague-Dawley Rats Elhemeidy, Rehab Mohammed Mustofa; Lyrawati, Diana; Widjajanto, Edi
Journal of Tropical Life Science Vol 8, No 3 (2018)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.08.03.04

Cellular immune system is one of anti-tumor target development. NK cells, one of major components of antitumor innate immune responses, are frequently decreased functionally during breast cancer progression, whereas TNF-alpha changes from anti-tumor into pro-tumorigenesis and its level increases with advancing cancer. In this study, we investigated the immunomodulatory effects of Ajwa date fruit extract to determine whether it stops the progression of breast cancer. Using rats induced with single dose 20 mg DMBA subcutaneously, we examined whether administration of Ajwa date fruit (Phoenix dactylifera) extract (at 400, 800 mg/kg body weight/d) post-DMBA induction for 30 days modulate NK cells, TNF-alpha, and development of breast cancer. ELISA, FACS, immunohistochemistry, and histologic observation were employed. Compared to positive control group (DMBA-induced only), we showed that the Ajwa date fruit extract normalize the level of circulatory CD161 NK cells and breast tissue TNF-alpha, cell size and proliferation, and improve overall survival rates. Ajwa date fruit extract may be used to modulate NK cells and TNF-alpha against progression of breast cancer. Further studies are required to develop date fruit extract as anti-cancer in human, whether as single or adjunct therapy to standard management of breast-cancer.

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