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Dentino: Jurnal Kedokteran Gigi
Published by Universitas Lambung Mangkurat
ISSN : 23375310     EISSN : 25274937     DOI : 10.20527
Core Subject : Health,
Dentistry
Dentino [P-ISSN 2337-5310 | E-ISSN 2527-4937] is the journal contains research articles and review of the literature on dentistry which is managed by the Faculty of Dentistry, Lambung Mangkurat University. Dentino published twice a year, every March and September.
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Articles 21 Documents
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Search results for , issue "Vol 4, No 2 (2019)" : 21 Documents clear
TOXICITY TEST OF THE MIXED MOUTHWASH OF MAULI BANANA STEM AND BASIL LEAF AGAINST FIBROBLAST CELL STUDY IN VITRO Martha Vania Saputri; Amy Nindia Carabelly; I Wayan Arya K. Firdaus
Dentino : Jurnal Kedokteran Gigi Vol 4, No 2 (2019)
Publisher : FKG Unlam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/dentino.v4i2.7043

Abstract

Background: Mauli banana stem and basil leaf are herbal plants which can be used as an herbal mouthwash. People in South Kalimantan often use mauli banana stem to accelerate wound healing because of its antibacterial activity. Basil leaf are proven to inhibit bacterial growth. Mauli banana stem has a bitter taste, so to minimize that bitter taste, basil leaf can be used as a mixture. Basil leaf give fresh and aromatic effect. Extract of mauli banana stem is not toxic against fibroblast cell BHK-21 with 25% concentrate, but there is no research about the toxicity of a mixed mouthwash of mauli banana stem and basil leaf against fibroblast cell BHK-21 yet. Purpose: To analyze IC50 after treatment with a mixed mouthwash of mauli banana stem and basil leaf into the fibroblast cell BHK-21. Method: True experimental using the posttest only with control group design which consisted of 10 treatment groups with several concentrations. Those were 31,25 μg/mL, 62,5 μg/mL, 125 μg/mL, 250 μg/mL, 500 μg/mL, 1000 μg/mL, 2000 μg/mL and 4000 μg/mL. The toxicity test used MTT Assay as a method. This research was formed by 2 stages, which were preparation of fibroblast cell BHK-21 and the toxicity test. The cell viability counted with Freshney formula. Result: The result showed that the IC50 was 2183,43 μg/mL. Conclusion: The mixed mouthwash of mauli banana stem and basil leaf is not toxic against fibroblast cell BHK-21 because IC50 is 2183,43 μg/mL.
THE EFFECT OF PROBIOTIC MILK IMMERSION ON BULK-FILL COMPOSITE RESIN DISCOLORATION Al Dina Yanuarita; Isyana Erlita; Buyung Maglenda
Dentino : Jurnal Kedokteran Gigi Vol 4, No 2 (2019)
Publisher : FKG Unlam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/dentino.v4i2.7060

Abstract

Backgrounds: Bulk-fill composite resin is a new type of composite resin that has the ability to absorb polymerization rays up to a depth of 4-5 mm in a single cure because of the modification in its components formation. The main cause of composite resin restorations failure is discoloration. Discoloration may occur due to extrinsic factors such as consuming food or drink which is rich in color and low pH, namely probiotic milk. Consuming probiotic milk for a long time will significantly increase the risk of composite resin discoloration. This significant discoloration eventually causes shorter resistance of restoration in oral environment regarding the decrease in aesthetic value. Objectives: To determine the effect of probiotic milk immersion on bulk-fill composite resin discoloration. Methods: This research was true experimental study with pretest-posttest with control group design that consisted of six groups in total of thirty six samples. Discoloration will be analyzed using digital analysis with the CIELAB method. Results: One-way ANOVA and Post-Hoc Games-Howell tests showed significant differences on bulk-fill composite resin discoloration after immersion in probiotic milk and distilled water (control) for 6, 12 and 18 hours (p<0,05). Only in the immersion groups of 6 hours and 12 hours probiotic milk did not have a significant difference on discoloration (p>0,05). Conclusion: There is an effect of probiotic milk immersion on bulk-fill composite resin discoloration.
DESCRIPTIONS OF CONDYLE HEAD POSITION IN DIGITAL PANORAMIC RADIOGRAPH OF CLICKING AND NONCLICKING PATIENTS AT RSGM UNPAD DENTAL RADIOLOGY INSTALLATION Meiryndra Syaira Putri; Farina Pramanik; Lusi Epsilawati
Dentino : Jurnal Kedokteran Gigi Vol 4, No 2 (2019)
Publisher : FKG Unlam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/dentino.v4i2.7056

Abstract

Background: Clicking is associated with the movement of condyle head and other predisposing factors. Digital panoramic radiograph may identify such condition by describing the position of head of condyle. Objective: To identify the description of condyle head position in digital panoramic radiograph of clicking and non-clicking patients at RSGM Unpad Dental Radiology Installation. Method: This was a descriptive research employing purposive sampling method to collect 31 samples of digital panoramic radiographs from 11 clicking patients and 20 non-clicking patients.  The measurement method in this research is referring to Ikeda’s (2011). Result: The average position of clicking TMJ was 1.45 mm from posterior aspect and the average position of non-clicking TMJ was 1.64 mm from superior aspect. Conclusion: It can be inferred that head of condyle’s position in digital panoramic radiographs of clicking and non-clicking patients at RSGM Unpad may change from normal position to the majority of head of condyle displacement approaching the glenoid fossa. Clicking TMJ is presented with posterior displacement while non-clicking TMJ demonstrates superior displacement.
TOXICITY TEST OF BAY LEAF EXTRACT ON BHK-21 FIBROBLAST CELLS IN VITRO Chintya Dewi Styo Ningrum; Debby Saputera; Rahmad Arifin
Dentino : Jurnal Kedokteran Gigi Vol 4, No 2 (2019)
Publisher : FKG Unlam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/dentino.v4i2.7067

Abstract

Background: Denture stomatitis is an inflammatory reaction in the oral mucosa that support the denture. This lesion is caused by the fungus Candida albicans and can be avoided by always maintain the cleanliness of denture. Bay leaf (Syzygium polyanthum) has flavonoid as the highest content which has antifungal and antioxidant properties so that bay leaf can be used as alternative ingredient for denture cleanser. Toxicity test needs to be done to determine the safety of this material. Objective: to analyze the toxicity of bay leaf extract (Syzygium polyanthum) to BHK-21 fibroblast cells using the MTT assay method. Method: This study is a true experimental study, which using posttest-only with control group design. The group, treated with bay leaf extract, were 25%, 20%, 15%, 10% and 5% concentrations including 2 control groups which were media control and cell control. Absorbance was read using ELISA reader and cell viability was calculated. Results: The percentage of living cells in all groups which treated with bay leaf extract was 100%. The parametric analysis of One Way Annova showed that there was no significant difference. Conclusion: Bay leaf extract (Syzygium polyanthum) does not have any toxic effect to BHK-21 fibroblast cell using the MTT assay method because cell viability in all treatment groups was ≥ 60%.
SURFACE HARDNESS EVALUATION OF BULK-FILL COMPOSITE RESIN AFTER IMMERSION IN PROBIOTIC DRINKS AND CARBONATED DRINKS Rema Rufaidah Qisthi; Isyana Erlita; Debby Saputera
Dentino : Jurnal Kedokteran Gigi Vol 4, No 2 (2019)
Publisher : FKG Unlam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/dentino.v4i2.7044

Abstract

Background: Probiotic drinks and carbonated drinks can affect the surface hardness of bulk-fill composite resin due to its acidic property. The acid may interfere with matrix and filler bonding in composite resin and will result in the dissolution of resin matrix and the decrease in hardness value of bulk-fill composite resin surface. Objective: The purpose of this study was to analyze the differences in bulk-fill composite resin surface hardness after the submergence in probiotic drinks and carbonated drinks. Methods: This study used pure laboratory experimental method (true experimental) with a post-test only and control group design. One Way ANOVA test followed by the Bonferroni Post Hoc test was performed to analyze the significance from each treatment. A total of 27 samples was divided into 3 groups, namely group 1 of bulk-fill composite resin soaked in probiotic drinks, group 2 soaked in carbonated drinks and group 3 soaked in sterile distilled water. The measurement of sample surface hardness was using Vickers Microhardness Tester. Results: The average value of surface hardness in group 1 was 61.41 Kg / mm2, group 2 was 57.62 Kg / mm2 and group 3 was 65.85 Kg / mm2. The results of One Way ANOVA parametric test obtained p = 0,000 (p <0.05) and continued with Bonferroni Post Hoc test which showed significant differences between each group (p<0.05). Conclusion: There is a lower effect of bulk-fill composite resin submergence in probiotic drinks on surface hardness than those immersed in carbonated drinks.
THE EFFECT OF TOPICAL TOMAN (Channa micropeltes) FISH EXTRACT ON THE NUMBER OF NEUTROFIL CELLS IN DIABETES MELITUS WOUND HEALING (In Vivo Study on Male Wistar (Rattus novergicus) Rat’s Back) Annisa Vara Nurdianty; Maharani Laillyza Apriasari; Amy Nindia Carabelly
Dentino : Jurnal Kedokteran Gigi Vol 4, No 2 (2019)
Publisher : FKG Unlam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/dentino.v4i2.7059

Abstract

Backgrounds: Diabetes mellitus forges the wound healing process to last longer. Toman fish which contains albumin and omega-6 fatty acid is proven to to enhance wound healing process in this systemic condition. Patients who suffer from diabetes mellitus retain high neutrophil number. Omega-6 fatty acids contained in Toman fish can decrease the number of  neutrophil cells. Objectives:  To prove the effect of Toman fish (Channa micropeltes) extract at 20% concentration topically on the number of neutrophil in diabetes mellitus-induced Wistar Rat (Rattus novergicus) injuries on day 4, 8 and 14.  Methods: This research was a true experimental study with posttest-only and control group with complete random design. Thirty six male Wistar rats (Rattus novergicus) was divided evenly for three treatments comprised of negative control given BR2 comfeed, positive control treated with Haruan fish (Channa striata) extraxt at 10% concentration topically and treatment group given with Toman fish (Channa micropeltes) extract at 20% concentration topically. Results: The data was analyzed using One Way ANOVA and was presenting the result of the 4th day (p=0.000), 8th day (p=0.001) and 14th day (p=0.000). Post-hoc Least Significant Difference (LSD) showed that p value was less than 0.05 which means that there was a significance difference in the mean of all treatment groups. Conclusion: Toman fish (Channa micropeltes) extract possesses the capacity in decreasing the number of neutrophil on 4th, 8th and 14th day in diabetic wound healing of Wistar Rat (Rattus novergicus).
THE EFFECTIVENESS OF OMEGA-3 MACKEREL FISH OIL ON THE DENSITY OF TOOTH ENAMEL In Vivo Study of White Rat (Rattus novergicus) Sandy Christiono; Arina Manashika; Erdianto Setya Wardhana
Dentino : Jurnal Kedokteran Gigi Vol 4, No 2 (2019)
Publisher : FKG Unlam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/dentino.v4i2.7058

Abstract

Background: Consuming cariogenic foods can cause calcium demineralization which is characterized by the appearance of dental caries. One effort on the prevention of dental caries is by consuming Mackerel fish oil that contains omega-3 fatty acids, omega-6 fatty acids, and vitamins that are beneficial for the body. Purpose: The study aimed to determine the effectiveness of omega-3 Mackerel fish oil on the density of tooth enamel in white rat (Rattus norvegicus). Method: This study applied an experimental research method with randomized posttest only control group design. Samples were comprised of 20 rats taken from 6 pregnant female rats which later divided into two groups: a treatment group administered orally with omega-3 Mackerel oil as much as 1ml / 200 mg BB and an aquadest control group. The treatment was administered during pregnancy period until the eruption of the teeth. Rats were euthanized to extract the tooth and enamel density was observed using micro-CT. The data were then analyzed with Levene’s homogeneity test and were preceded to independent T-test. Result: The average enamel density of the treatment group was 1155.18340 while the control group was 175.91640. Based on independent t test between the two groups, a significant difference in tooth enamel density of Rattus Noevegicus was obtained (p <0.05). Conclusion: The administration of omega-3 Mackerel fish oil may increase the density of tooth enamel in White Rat (Rattus norvegicus).
PHASE TRANSFORMATION OF CASO4 GRANULES TO CACO3 GRANULES BY DISSOLUTION–PRECIPITATION REACTION WITH 12 HOURS IMMERSION TIME Difa Putri Utami; Decky Joesiana Indrani; Bambang Irawan; Sunarso Sunarso
Dentino : Jurnal Kedokteran Gigi Vol 4, No 2 (2019)
Publisher : FKG Unlam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/dentino.v4i2.7052

Abstract

Background: Synthetic bone graft is a material that resembles human bone phase and is developed due to clinical demand. Calcium carbonte (CaCO3/Calcite) has been used as bone substitution one of the methods to fabricate calcite is phase transformation by dissolution–precipitation reaction.  Previous study did the same method but with lower temperature (<100ºC). Calcium sulfate anhydrate (CaSO4) granules used as precursor is immersed in 0.5 mol/L sodium carbonate (Na2CO3) solution at 100ºC for 12 h.  Objective: This study aims to fabricate CaCO3 granules from CaSO4 granules when the temperature is higher than the previous study. Methods: Fabricate CaCO3 granules using CaSO4 granules as precursor by dissolution-precipitation reaction in Na2CO3 solution with 12 h immersion time with 100ºC temperature. Powder X-ray diffraction patterns and Fourier transform infrared spectra study will be performed to characterize the granules. Results:  CaCO3 granules are fabricated by dissolution-precipitation reaction in Na2CO3 solution with 12 h immersion time when the temperature was 100ºC. Conclusion: CaSO4 granules used as precursor are a potential material to fabricate CaCO3 by using dissolution-precipitation reaction with 12 hours immersion time and 100ºC temperature.
ANTIBACTERIAL ACTIVITIES OF CHITOSAN IN HARUAN FISH SCALES (Channa striata) TO THE GROWTH OF Staphylococcus aureus Dewi Retno Wahyu Widyaningrum; Deby Kania Tri Putri; Irham Taufiqurrahman
Dentino : Jurnal Kedokteran Gigi Vol 4, No 2 (2019)
Publisher : FKG Unlam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/dentino.v4i2.7045

Abstract

Background: Haruan fish scales (Channa striata) contain chitin which can be used as antibacterial ingredients by processing it into chitosan. Chitosan contains an amino polysaccharide group which can inhibit the growth of Staphylococcus aureus, one of the opportunistic bacteria, which becomes a pathogen if there is an imbalance within oral environment and one of the causes of periodontal disease. Besides, these bacteria are also reported to be resistant to conventional antibacterial agents. Objective: The purpose of this study was to find out and elaborate the difference in antibacterial activity of chitosan from haruan fish scales (Channa striata) at the concentration of 0.5%; 1%; 1.5%; 2%; 2.5%; 3%; 3.5%; 4% on the growth of Staphylococcus aureus. Methods: This study used a randomized pretest-posttest with control group design and One Way ANOVA statistical test was performed and was followed by Post Hoc Dunnet test. The procedure of this study consisted of chitosan fabrication scales from fresh fish by deproteination, demineralization, and deacetylation, followed by an anti-bacterial test with 10 treatment groups namely 0.5%; 1%; 1.5%; 2%; 2.5%; 3%; 3.5%; 4% on the growth of Staphylococcus aureus to obtain Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC). Results: The results of this study presented chitosan at 1.5% concentration as the Minimum Inhibitory Concentration (MIC) and 3.5% concentration as the Minimum Bactericidal Concentration (MBC) on the growth of Staphylococcus aureus. Evaluated from the difference in absorbance, 2.5% chitosan concentration showed superior activity in inhibiting Staphylococcus aureus compared to 0.2% chlorhexidine gluconate. Conclusion: It can be concluded that there are differences in the antibacterial activity of chitosan scales (Channa striata) with a concentration of 0, 5%; 1%; 1.5%; 2%; 2.5%; 3%; 3.5%; 4% on the growth of Staphylococcus aureus.
COMPARISON OF INHIBITORY ACTIVITY OF KELAKAI LEAF EXTRACT AND 0.2% Chlorhexidine Gluconate AGAINST Streptococcus sanguinis ATCC® 10556™ Fitriana Caessar Pertiwi; I Wayan Arya K. Firdaus; Isyana Erlita
Dentino : Jurnal Kedokteran Gigi Vol 4, No 2 (2019)
Publisher : FKG Unlam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/dentino.v4i2.7066

Abstract

Background: Gingivitis is an early periodontal disease that occurs due to host response and dental plaque. Streptococcus sanguinis is pioneer of dental plaque colonization in oral cavity. Gingivitis treatment use 0,2% Chlorhexidine gluconate mouthwash, but long-term use causes side effects, so an alternative herbal mouthwash which has minimal side effects is needed. Kelakai leaf ethanol extract contains antibacterial compound, such as flavonoid, alkaloid, tannin and steroid, which can be herbal-based mouthwash as an alternative of 0,2% Chlorhexidine gluconate. Purpose: It was to compare the inhibitory zone of kelakai leaf ethanol extract and 0,2% Chlorhexidine gluconate against S. sanguinis. Materials and methods: This study was true experimental research and post-test only with control group design, that used 5 treatment groups with 4 replications of kelakai leaf ethanol extract, that were 25%, 50%, 75%, 100% concentrations and 0,2% Chlorhexidine gluconate against S.sanguinis with 20 total samples. All groups incubated for 24 hours at 37oC, then it measured the diameter of inhibition zone using calliper. Result: One Way ANOVA and Post Hoc LSD tests showed significant difference between all groups after given with kelakai leaf ethanol extract and 0,2% Chlorhexidine gluconate. Kelakai leaf ethanol extract 25%, 50%, 75%, 100% concentrations and 0,2%  Chlorhexidine gluconate has an average inhibition zone, which were 9,00 mm, 11,20 mm, 13,67 mm, 16,32 mm, and 18,12 mm against S.sanguinis. Conclusion: Kelakai leaf ethanol extract 25%, 50%, 75% and 100% concentrations has antibacterial activity against S.sanguinis, but not as strong as 0,2% Chlorhexidine gluconate.

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