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International Journal of Biosciences and Biotechnology

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Published by Universitas Udayana

ISSN : 23033371 EISSN : 26559994 DOI : -

Core Subject : Science, Social,

Biochemistry, Genetics & Molecular Biology Environmental Science

International Journal of Biosciences and Biotechnology provides a unique venue for publishing original researches in biosciences and biotechnology, and ensures that authors could reach the widest possible audience. It publishes both full-length articles and short communications on all aspects of biotechnology and biosciences

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Articles 5 Documents

Search results for , issue "Vol 4 No 2 (2017)" : 5 Documents clear

ISOLATION AND IDENTIFICATION OF Pseudomonas spp. TO CONTROL Plasmodiophora brassciae, THE PATHOGEN OF CLUBROOT DISEASE ON CABBAGE I Ketut Suada; Anak Agung Ngurah Gede Suwastika
International Journal of Biosciences and Biotechnology Vol 4 No 2 (2017)
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University in cooperation with Asia-Oceania Bioscience and Biotechnology Consortium (AOBBC)

Clubroot is very detrimental disease to cabbage production so as farmers work on various efforts to control it. The use of fungicides not only ineffective but also pollute the environment, therefore biological control system need to be pursued. The use of antagonistic agents such as Pseudomonas has been widely studied and known effective in suppressing various pathogens. Therefore it is worth trying its effectiveness against Plasmodiophora brassicae, a pathogen of cabbage. The purpose of this study was to obtain indigenous Pseudomonas which effectively suppress the pathogens and may also increase plant growth. Microbes were isolated from the cabbage area using the Kings'B medium with multilevel dilution. All isolates were tested for their effectiveness in pots in a Completely Randomized Design with a concentration of 1.5x106 CFU (Colony Forming Unit) per pot. The variables observed were plant growth, number of club roots, and percentage of disease incidence. Fourteen isolates of Pseudomonas were isolated. Three Pseudomonas isolates were found most effective at suppressing clubroot disease and increasing plant growth. The best isolate obtained was Pseudomonas-6, followed Pseudomonas-9, and Pseudomonas-8.

CROP IMPROVEMENT THROUGH INDUCING MUTAGENESIS IN VIVO USING COLCHICINE ON COWPEA (Vigna unguiculata L. Walp) I Gede Ketut Susrama; I Gede Putu Wirawan
International Journal of Biosciences and Biotechnology Vol 4 No 2 (2017)
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University in cooperation with Asia-Oceania Bioscience and Biotechnology Consortium (AOBBC)

Colchicine treatments of 0.005% and 0.01% either in distilled water or in hydrogen peroxide 3% were caused temporary leaf shape changes on cowpea. Treatment of colchicine in hydrogen peroxide 3% resulted in green pod color change to purple however treatment of just hydrogen peroxide 3% and colchicine in hydrogen peroxide 3% caused 8.8-10.7% dwarf cowpea. As a preliminary data, we also observed that cowpea crops grown from seeds treated with colchicine in distillate water attacked by bean leaf beetle much earlier compare to the treatment with colchicine in hydrogen peroxide 3%.

THE QUALITY AND TOTAL MICROBES OF BROILER CHICKEN IN TRADITIONAL MARKETS OF DENPASAR CITY Luh Yuni Surya Antari; I N. Tirta Ariana; G. A. M. Kristina Dewi
International Journal of Biosciences and Biotechnology Vol 4 No 2 (2017)
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University in cooperation with Asia-Oceania Bioscience and Biotechnology Consortium (AOBBC)

The purpose of this study was to determine the quality and total microbes of broiler at different location in the traditional markets of Denpasar City. This study used a completely randomized design (CRD) with four treatments. The treatments used in this study, broiler chicken sold at traditional markets in East (DT), North (DU), West (DB), and South of Denpasar (DS). The data obtained from this study were analyzed by analysis of variance. If there were significant differences (P <0,05) among the treatments, then continued with Duncan's multiple range test. The variables used in this study were carcass weight, cooking loss, drip loss, water holding capacity, water content, protein, fat, ash, and total microbes. The results showed that on the average, the highest carcass weight in DB treatment was 1.482 grams, with other treatments to get results not significantly different (P>0,05). The result of this study for variable drip loss, water content, protein, fat, and ash significantly different (P<0,05), then research result for cooking loss, and water holding capacity, were not significantly different (P>0,05). The research result for total microbies broiler chicken at traditional markets in Denpasar City have a range of 1,84 x 104 to 2,62 x 104 CFU /gram. The results were still normal when compared to SNI 7388: 2009 which states that the maximum amount that the content of TPC 1x106 CFU/gram. From these results, it was concluded that there were similarities quality of broiler chicken (carcass weight, cooking loss, water holding capacity, and total microbes), however, there were differences in the quality of broiler seen from drip loss, water content, protein, fat, and ash of broiler at traditional markets in Denpasar City.

MINERAL FERTLIZER AS AN ALTERNATIVE FERTILIZER IN INCREASING RICE YIELD IN TABANAN REGENCY Indayati Lanya; N. Netera Subadiyasa
International Journal of Biosciences and Biotechnology Vol 4 No 2 (2017)
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University in cooperation with Asia-Oceania Bioscience and Biotechnology Consortium (AOBBC)

Production can be increased through improved harvest index genetic engineering, and the availability of nutrients in the soil. Ciherang rice production tests conducted over two years by toposekuen in Tabanan. First in five Subak, (Jangga, Bulung Daya, Andal Dewa, Lanyah1, and Perean) with 10 fertilizer treatments. The second year, five fertilization treatments in the two Subak (Petung dan Babahan). The study was conducted in randomized complete block design with three replications. Parameters observed included vegetative and generative growth, and yield of paddy field farming. Duncan`s test, with a confidence level of 5% using the Costat program, and input output fertilization. Fertilization is very significant effect on rice production. The first year results, the highest rice production (9.33 tons ha-1) was achieved in a combination of fertilizer treatment (organic + NPK + mineral), can increase production 43.98%. The second year of the highest rice production (11.878 tons ha-1) were achieved on the fertilization of NPM (100 kg urea + 100 kg Phonska ha-1) + organic fertilizer (2.5 tons ha-1) + mineral fertilizer (2.5 tons ha-1), production increased 50.4%. Organic fertilization received the lowest rice production (5,420 to 8,940 tons ha-1), and the highest percentage of empty grain (9.29%). Mineral fertilizers can reduce the use of chemical fertilizers and high-dose organic fertilizers. The increase in yield of paddy field farming compared to control for the treatment of organic fertilizer (Rp 1.011 million ha-1), NP (Rp 6.416 million ha-1), NPK (Rp7.636 million ha-1), and NPM (Rp12.576 million ha -1).

HEXOKINASES IN BREAST CANCER S. Kharitonov; A. Zikiriahodzhaev; M. Ermoshchenkova; A. Sukhot’ko; M. Fedorova; E. Pudova; B. Alekseev; A. Kaprin; A. Kudryavtseva
International Journal of Biosciences and Biotechnology Vol 4 No 2 (2017)
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University in cooperation with Asia-Oceania Bioscience and Biotechnology Consortium (AOBBC)

Hexokinases are one of the key enzymes involved in the process of glycolysis. The level of expression of hexokinases is widely studied in breast cancer as a possible marker of unfavorable prognosis and aggressiveness of tumors. The level of expression of hexokinase may reflect the level of glycolysis activation and, thus, indicate samples with the most altered cellular metabolism.

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