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International Journal of Biosciences and Biotechnology

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Published by Universitas Udayana

ISSN : 23033371 EISSN : 26559994 DOI : -

Core Subject : Science, Social,

Biochemistry, Genetics & Molecular Biology Environmental Science

International Journal of Biosciences and Biotechnology provides a unique venue for publishing original researches in biosciences and biotechnology, and ensures that authors could reach the widest possible audience. It publishes both full-length articles and short communications on all aspects of biotechnology and biosciences

Arjuna Subject : -

Articles 160 Documents

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PHYTOREMEDIATION OF MERCURY CONTAMINATED SOILS IN A SMALL SCALE ARTISANAL GOLD MINING REGION OF INDONESIA Kokyo Oh; Sachiko Takahi; Sri Wedhastri; Hardita Librasanti Sudarmawan; Retno Rosariastuti; Irfan Dwidya Prijambada
International Journal of Biosciences and Biotechnology Vol 3 No 1 (2015)
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University in cooperation with Asia-Oceania Bioscience and Biotechnology Consortium (AOBBC)

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In the small gold mining regions of Indonesia, the tailings or waste water containing mercury commonly may be released into agricultural lands resultimg soil contamination. Phytoremediation is a low-cost and environmental friendly alternative to traditional techniques such as soil heating, soil removal, and soil washing. In this study, a sweet sorghum combined with the inoculation of a rhizobacteria, Agrobacterium tumefaciens, was tested in a field experiment with mercury contaminated soil from a small scale gold mining. Plant growth, uptake and accumulation of mercury by the sweet sorghum, and effects of the Agrobacterium tumefaciens inoculation on mercury accumulation were investigated. The average of mercury content in the soil was 3.76 mg/kg. The results showed that the sorghum grew well, and the inoculation of Agrobacterium tumefaciens promoted the plant growth, but did not increased the mercury concentration in both root and stem parts of the sorghum. The accumulation of mercury was 6.2?/plant for sorghum without Agrobacterium tumefaciens, and 14.0?/plant for sorghum with Agrobacterium tumefaciens. It was estimated that the phytoremediation efficiency of mercury was 414 and 934 mg/ha for sweet sorghum without Agrobacterium tumefaciens inoculation and with Agrobacterium tumefaciens inoculation, respectively.

ANALYSIS ON RELATIONSHIP AMONG DENDROBIUM spp BALI BASED ON CHARACTERISTICS OF LEAVES ANATOMY Ida Ayu Putri Darmawati; I Nyoman Rai; Rindang Dwiyani; Ida Ayu Astarini
International Journal of Biosciences and Biotechnology Vol 5 No 2 (2018)
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University in cooperation with Asia-Oceania Bioscience and Biotechnology Consortium (AOBBC)

From explorations in several parts of the forest of Bali, 24 species of Dendrobium orchids were identified. Relationship among these species needed to be mapped for further development of orchids. Characteristics of leaves anatomy have been used to determine relations among plants. Characteristics of anatomy that were observed in this study from the paradermal and transversal cut of the leaves were shape and number of epidermis cells, location of stomata, the direction of stomata opening, shape and number of stomata, length of the epidermis and stomata, density of stomata, index of stomata, and thickness of mesophyll. Analysis of relations among species was displayed as a dendogram, using the software Minitab 17 Vis. Analysis of relationship among 24 species of Dendrobium Bali based on the leaves anatomy used grouped average method in squared Euclidean distance with the scale of 0.349 to 0.972. At Euclidean distance of 0.349, two clusters were formed where D. aloifolium and D. subulatum were separated from the other clusters. At 80 % similarity coefficient, seven clusters were formed using similar method, with the 3rd and 5th clusters had the shortest distance (169.35). If species that were clustered together were cross-bred, the success rate was higher; meanwhile, the further away the relation between species, the smaller the success rate of cross breeding. The possibility of getting high-quality genotypes was higher when the cross-breeding was successful.

Content Articles Content Articles
International Journal of Biosciences and Biotechnology Vol 4 No 1 (2016)
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University in cooperation with Asia-Oceania Bioscience and Biotechnology Consortium (AOBBC)

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Content Articles

ASSESSMENT OF LIFE QUALITY OF CERVICAL CANCER PATIENTS DELIVERED BLEOMYCIN ONCOVIN MITOCYN PLATINUM (BOMP) CHEMOTHERAPY REGIMENS IN SANGLAH DENPASAR Rini Noviyani; I Nyoman Gede Budiana; Putu Ayu Indrayathi
International Journal of Biosciences and Biotechnology Vol 2 No 2 (2015)
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University in cooperation with Asia-Oceania Bioscience and Biotechnology Consortium (AOBBC)

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Cervical cancer disease is one of cancers that attack many women in the world and ranks second afterbreast cancer. Symptoms of cervical cancer may cause degradation quality of patients life.Chemotherapy is one of treatments of cervical cancer patients. Chemotherapy provides therapeuticeffects, however, it can causes a decrease in life quality due to its side effects. BOMP is one ofregimens that can be used as one of the management of cervical cancer. Assessment of quality ofpatients life with cervical cancer are needed to see the changes in the quality of life in patients besidesknowing the functional status changes from time to time. Monitoring the effects of treatment, andcollecting the data on quality of life can be used as initial data for consideration in formulating theappropriate action for the patient, And also assist physicians in selecting an effective and welltolerated drug for patients.The study was conducted in February to June 2014 in Obstetrics Clinic andCempaka Timur Room General Hospital (RSUP) Sanglah in observational design with cross sectionalprospective. The sample selection is done by consecutive sampling. The methods used for datacollection using questionnaires EORTC QLQ with interview techniques before and afterchemotherapy in patients suffering cervical cancer of squamous cell stage IIB - IIIB who had BOMPchemotherapy regimens for 3 series in RSUP Sanglah. Research for quality of life is conducted ingeneral and the 15 domains that affect the quality of life of patients. In this study, 12 patients indicatedthe inclusion criteria. There were significant differences in the value of the quality of life of patientswith cervical cancer squamous cell stage IIB - IIIB before and after BOMP chemotherapy with 0.001p value. BOMP chemotherapy regimens can improved the quality of life of cervical cancer patientsincreasing from 44.833 ± 6.235 to 60.333 ± 9.168. In 15 ratings domain included decrease the qualityof life of pain domain, nausea domain, vomiting domain, decreased appetite domains, fatigue domain,physical function domain, functional role domain, social functioning domain, sleeplessness domain,constipation domain and domain of financial difficulties and on the other hand emotional domainincrease.

forewords forewords forewords
International Journal of Biosciences and Biotechnology Vol 2 No 1 (2014)
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University in cooperation with Asia-Oceania Bioscience and Biotechnology Consortium (AOBBC)

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forewords

IN VITRO CALLUS INDUCTION OF ‘SHIRAZ’ GRAPE (Vitis vinifera L.) USING DIFFERENT MEDIUM AND GROWTH REGULATOR COMBINATION Ni Nyoman Nila Arieswari; Ida Ayu Astarini; Ni Putu Adriani Astiti; Jeremy Pramana
International Journal of Biosciences and Biotechnology Vol 6 No 1 (2018)
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University in cooperation with Asia-Oceania Bioscience and Biotechnology Consortium (AOBBC)

One of grape cultivars widely used as raw material for wine production is ‘Shiraz’ cultivar. Propagation of this cultivar is necessary for the provision of grape as a wine making material in Bali. In vitro culture is an alternative propagation technique than can be employed to produce planting materials in a shorter time. This research aims to determine the most suitable medium and growth regulator combination in inducing grape cv. ‘Shiraz’ callus in vitro. The study was conducted from November 2017 until February 2018 at Plant Tissue Culture Laboratory, Biology Study Program, Udayana University. The explants used were young stem of grape cv. ‘Shiraz’ and the experiment was conducted using factorial design with two factors. The first factor was basal medium used (MS and WPM) and the second factor was IBA concentration (0; 0.5 and 1 mgL-1) and BAP (0, 1 and 2 mgL-1). The results showed that the highest percentage of callus induction (60%) was obtained on WPM medium without growth regulator combination (control). However, the fastest time of callus appear was on MS medium + 2 mgL-1 BAP without IBA, which was 17 days after planting. The texture and color of callus resulted on this research were friable with white, greenish white, greenish yellow and green in color.

GENETIC DIVERSITY OF GUAVA (Psidium Guajava L.) IN BALI INDONESIA BASED ON RAPD MARKER Ni Nyoman Ari Mayadewi; I Nyoman Rai; Rindang Dwiyani; Ida Ayu Astarini
International Journal of Biosciences and Biotechnology Vol 4 No 1 (2016)
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University in cooperation with Asia-Oceania Bioscience and Biotechnology Consortium (AOBBC)

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The aim of this study was to investigate genetic diversity and relationship of guava (local name: jambu biji) genotypes grown in Bali, Indonesia, based on Random Amplified Polymorphic DNA (RAPD) markers. Twelve guava cultivars namely Jambu biji ‘Australia’, ‘Bangkok Merah’, ‘Bangkok Putih’, ‘Cokorde’, ‘Dadu 1’, ‘Dadu 2’, ‘Getas Merah’, ‘Kamboja’, ‘Kristal’, ‘Pipit’, ‘Susu’ and ‘Variegata’ were collected from nine Regencies in Bali using survey and explorative method. Ten decamers RAPD primers were employed to distinguish between the 12 cultivars and determine their genetic relationships. A dendogram was constructed using coefficient dissimilarity analysis based on phylogenetic analysis using parsimony (PAUP). The twelve cultivars were grouped into 2 main clusters and five smaller clusters. Variation between genotypes of guava local will be good sources for future crop improvemen.

THE EXISTENCE OF ENTOMOPATHOGENIC FUNGI ON RICE PLANTS RHIZOSPHERE Noerfitryani Noerfitryani; Hamzah Hamzah
International Journal of Biosciences and Biotechnology Vol 5 No 1 (2017)
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University in cooperation with Asia-Oceania Bioscience and Biotechnology Consortium (AOBBC)

Enthomophatogenic fungus is biological control. This research aimed to identify fungi on semi-organic and inorganic rice plants rhizosphere and proved as entomophatogenic fungi. This research was conducted in Biological control, Plant Pests and Diseases Department, Faculty of Agriculture, Universitas Hasanuddin, Makassar. Isolation of fungi with a modified insect bait method then tested in larvae, calculated mortality of larvae, Tenebrio molitor were infected by fungi were re-isolated and cultured in PDA media. The result showed that the average of T. molitor larvae mortality infected by enthomopathogenic fungi on semi-organic rhizosphere was around 24% and 8% on inorganic rhizosphere. The percentage mortality of larvae on semi-organic rhizosphere were infected by Fusarium sp. was around 20-60 %, Aspergillus sp. 60%-80% and Trichoderma sp. 80%, while in inorganic rhizosphere up to 100% were infected by Aspergillus spp.

APPLICATION OF NATURAL COLOR MIXED AND NaCl AS MORDANT ON WOOD FIBER USING SIMULTANEOUS MORDANTING Ni Wayan Bogoriani
International Journal of Biosciences and Biotechnology Vol 3 No 2 (2016)
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University in cooperation with Asia-Oceania Bioscience and Biotechnology Consortium (AOBBC)

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Wood is one of the few materials that is highly exploited by humans, resulting in increasing usage of low quality wood. High demand and usage on high-quality wood causing heavy reduction of its availability. To increase artistic value of low quality wood, staining on the surface of the wood need to be done. This research aims to apply a mix of natural pigments (Gambier- Piper betle leaves- Areca seeds) and NaCl salt as the mordant on the surface of Albesia wood fiber and test the physical and chemical dye properties with 1% detergent. This study uses a mixture of natural dyes (Gambier- Piper betle leaves- Areca nut) with various concentrations and salt as auxiliaries, carried out with the simultaneous method of mordanting. This study was divided into 7 groups. Differences in mean between groups were tested using One Way ANOVA followed by Post Hoc LSD, which revealed statistically significant test when p value < >0.05. The results showed that there were significant differences of natural dyes mixed applications (Gambier- Piper betle leaves- Areca seeds) in the ratio of 5.0: 3.0: 3.0 to 0.5 g of salt as auxiliaries and 10 minutes of immersion with the simultaneous method of mordanting. Ratio of the dye mixture, on the surface of the wood causes brown color and has the highest absorption average of 0.19 gram and the durability of the dye showed the highest seen from the appearance of color and mix of the missing mass. It can be concluded that the best application is with a mixture of natural dyes and the addition of 0.5 g of NaCl salt on the surface of Albisia wood fiber with the simultaneous method of mordanting and also based on the durability of the dye test is a ratio of 5.0: 3.0: 3.0.

THE WATER EXTRACT OF Saragassum fusiforme IS A POTENTIAL ELICITOR OF INDUCED RESISTANCE IN SOLANUM LYCOPERSICUM BUT NOT IN Nicotiana benthamiana Layth Sbaihat; Daigo Takemoto; Kazuhito Kawakita
International Journal of Biosciences and Biotechnology Vol 2 No 1 (2014)
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University in cooperation with Asia-Oceania Bioscience and Biotechnology Consortium (AOBBC)

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Molecules called elicitors induce plants’ defense responses.Elicitor -??induced resistance rarely leads tocomplete pathogen control, but reduce lesion size and/or number instead. We investigated a novel elicitorextractedfrom the brown sea algae (Saragassum fusiforme), whichis sowed to induce reactive oxygen speciesproduction and protection against powdery mildew and late blight diseasesoftomato (Solanum lycopersicum,cultivar Lady First)plants. On the other hand, the studied elicitor did not induce reactive oxygen speciesproduction in Nicotiana benthamiana plants nor protected them against late blight disease .

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