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International Journal of Biosciences and Biotechnology

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Published by Universitas Udayana

ISSN : 23033371 EISSN : 26559994 DOI : -

Core Subject : Science, Social,

Biochemistry, Genetics & Molecular Biology Environmental Science

International Journal of Biosciences and Biotechnology provides a unique venue for publishing original researches in biosciences and biotechnology, and ensures that authors could reach the widest possible audience. It publishes both full-length articles and short communications on all aspects of biotechnology and biosciences

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Articles 160 Documents

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THE MOLECULAR DETECTION OF CITRUS VEIN PHLOEM DEGENERATION (CVPD) PATHOGEN (Liberobacter asiaticus) IN Diaphorina citri KUWAYAMA (HOMOPTERA : PSYLLIDAE) AND OTHER INSECTS ASSOCIATED WITH CITRUS PLANT I Nyoman Wijaya; Wayan Adiartayasa; Made Sritamin
International Journal of Biosciences and Biotechnology Vol 5 No 2 (2018)
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University in cooperation with Asia-Oceania Bioscience and Biotechnology Consortium (AOBBC)

The research was coducted at Laboratory of Biotechnology Faculty of Agriculture Udayana University. The purpose of this research were to investigate moleculer detection of CVPD pathogen in its vector, other phytophagous insects on citrus cv, Siam and kemuning (Murraya paniculata L. Jack.). The result of this research showed that adults and nymphs of Diaphorina citri Kuw. could transmit CVPD disease, but the pathogen of CVPD could not be transovarially transmited. As a host of CVPD disease , kemuning could be source of infection. The adult of Toxoptera citricidus Kirkaldy and Maleuterpes dentipes Hell. could not potensial transmited CVPD disease.

CROP IMPROVEMENT THROUGH INDUCING MUTAGENESIS IN VIVO USING COLCHICINE ON COWPEA (Vigna unguiculata L. Walp) I Gede Ketut Susrama; I Gede Putu Wirawan
International Journal of Biosciences and Biotechnology Vol 4 No 2 (2017)
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University in cooperation with Asia-Oceania Bioscience and Biotechnology Consortium (AOBBC)

Colchicine treatments of 0.005% and 0.01% either in distilled water or in hydrogen peroxide 3% were caused temporary leaf shape changes on cowpea. Treatment of colchicine in hydrogen peroxide 3% resulted in green pod color change to purple however treatment of just hydrogen peroxide 3% and colchicine in hydrogen peroxide 3% caused 8.8-10.7% dwarf cowpea. As a preliminary data, we also observed that cowpea crops grown from seeds treated with colchicine in distillate water attacked by bean leaf beetle much earlier compare to the treatment with colchicine in hydrogen peroxide 3%.

UTILIZATION OF RHIZOSPHERE FUNGI TO CONTROL Fusarium oxysporum f.sp. capsici IN VITRO I Made Sudarma; Ni Made Puspawati; Ni Wayan Suniti; I Nyoman Wijaya; I Gusti Ngurah Bagus
International Journal of Biosciences and Biotechnology Vol 2 No 2 (2015)
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University in cooperation with Asia-Oceania Bioscience and Biotechnology Consortium (AOBBC)

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Fusarium caused wilt disease in chilli pepper and destroyed some farmer crops. Results ofpreliminary research has been discovered that the disease caused by Fusarium oxysporum f.sp. capsici.The alternative environmental friendly method is to find antagonist microbes which is located in therhizosphere of healthy pepper plants. This study aims to find out potentially antagonistic fungi tocontrol Fusarium wilt disease on pepper plants. The fungi were isolated by soil dilution method orviable plate count method on Potato Dextrose Agar medium with antibiotic Livoplaxacin (25%, w/v).Rhizosphere fungi from healthy pepper plants had been identified. A total of 63 spesies belong to 4genera included Penicillium (45 species), Aspergillus (6 species), Trichoderma (9 species) andCandida (3 species). The highest percentage of distribution of rhizosphere fungi are P. digitatum(47.63%), P. expansum (19,05%), T. harzianum (9,53%), A. nidulans, A. niger, Penicillium sp.,Candida albicans, and T. vitrens i.e 4,76% respectively. All of rhizosphere fungi colonies were foundto inhibit Fusarium oxysporum f.sp. capsici in vitro. The best inhibition was found in Aspergillusniger at 88.89 ± 2.2% followed by A. nidulans of 85,56 ± 1,6 %, T. harzianum at 84,45 ± 1,58% , andT. virens by 83,33 ± 1,2%, five days after inoculation. All of them have a very high inhibition criteria.

AGROECOSYSTEMS OF LAND SUITABILITY IN SUB-DISTRICT OF SOUTH KUTA FOR COMMODITY DEVELOPMENT HORTIKULTURA FRUITS IN BADUNG DISTRICT, BALI Ni Made Trigunasih; I Nyoman Dibia
International Journal of Biosciences and Biotechnology Vol 6 No 2 (2019)
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University in cooperation with Asia-Oceania Bioscience and Biotechnology Consortium (AOBBC)

This study aims to: (1) to know the suitability of the land for the development of several fruit horticultural commodities in the Sub-district of South Kuta, (2) determine the suitability of land in agroecosystem and its limiting factors, (3) provide ecommendations for land management in the study area. The study used a field survey method with the determination of observation samples carried out in a stratified purposive sampling with land units as the strata. Land quality/characteristics as a determinant of land suitability observed include: annual mean temperature, water availability; rooting media; availability of oxygen; nutrient retention, CEC, BS, pH, and organic matter; nutrients available; erosion; flood hazard; land preparation. Land suitability assessment is done by matching the land quality with the growing requirements for the plants evaluated. The results showed that the actual land suitability for food crops of fruit horticulture (citrus, mango, starfruit, banana, sapodilla, grape, klengkeng, papaya, grain guava, guava, jackfruit, soursop, avocado, srikaya, breadfruit and pineapple) belonged according to conditional marginal suitable (S3), until not appropriate (N) with the quality of the land as a limiting factor are: rooting media (effective depth of soil); land preparation (surface rocks and rock outcrops); erosion hazard; and nutrient availability (especially N-total and P-available). The assumption is that some land quality/characteristics that are as limiting factors can be improved, then the suitability of the potential land is quite suitable (S2) to marginal suitable (S3) with the quality/characteristics of the land as a limiting are: availability of water (long dry months), rooting media (effective depth), land preparation (surface rocks, rock outcrops), and nutrient retention (rather high soil pH). Increased land productivity in the study area can be done by improving land management by providing inputs such as: the addition of sufficient organic material, adition of fertilizer containing elements of N (ZA), and fertilizer containing elements of Potasium (SP36), taking conservation measures in the form of making terraces on sloping lands.

CLONING OF FLOWERING GENES (WJFLC AND WJFT) IN WASABI (JAPANESE HORSERADISH) AND MONITORING OF FLOWERING RESPONSE WITH THEIR EXPRESSION Masayuki Nozue; Hiroyoshi Kubo; Kiyoshi Yoshida
International Journal of Biosciences and Biotechnology Vol 1 No 2 (2013)
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University in cooperation with Asia-Oceania Bioscience and Biotechnology Consortium (AOBBC)

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Wasabi (Wasabia japonica) is a commercially important crop in Japan. We isolated a FLC ortholog (WjFLC)and FT ortholog (WjFT) from wasabi. Predicted amino acid sequence encoded by WjFLC and WjFT showed89% and 85% identities with FLC and FT of Arabidopsis, respectively. The expression of WjFLC was high inOctober and reduced in November when fl ower buds are formed in wasabi. On the other hand, expressionof WjFT was not detected in October and was slightly detected in November. Thereafter, WjFT was highlyexpressed later in February. We examined the best condition for initiation of fl ower bud formation undervarious artifi cial environments by monitoring the fl owering response of wasabi using WjFLC and WjFT.

SCIENCE AND TECHNOLOGICAL ASPECTS IN RELIGIOUS RITUALS IN THE WOMEN FARMERS GROUP OF JEMPIRING KABUPATEN BADUNG, BALI, INDONESIA Ni Wayan Suryathi; Made Antara; Nengah Bawa Atmaja; Wayan Windia
International Journal of Biosciences and Biotechnology Vol 6 No 1 (2018)
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University in cooperation with Asia-Oceania Bioscience and Biotechnology Consortium (AOBBC)

This study aims to analyse the science and technological values in religious rituals in the women farmers group of Jempiring Kabupaten Badung. The data was analysed qualitatively. The results show: science and technological values in religious rituals which are implemented through Tri Hita Karana which are harmonious relationship with God, harmonious relationship with other people, and harmonious relationship with the environment, it is evident from Banten Pejati that is complete with segehan Agung which is given on piodalan at Pura Melanting. Ans it is also followed by pemerastitaan and pelukatan rituals for the group’s members. Daksina in Banten Pejati is the symbol of God’s palace. This symbol provides a scientific-psycho-theological effect that makes the minds of the people moored in the presence of a metaphysical god in the support of physical daksina. This value is also found in the sound of kul-kul, genta, gamelan, kidung and mantra during rituals. A harmonious relationship with other people is also seen from the behavior of good and honest members with a basis of menyame braya.

PHOSPHATASE ACTIVITY AND PHOSPHATE SOLUBILITY BY PHOSPHATE SOLUBILIZING RHIZOBACTERIA IN VOLCANIC SOILS OF PANCASARI, BALI Ketut Dharma Susila; I Made Sudana; Ni Putu Ristiati; I Made Adnyana
International Journal of Biosciences and Biotechnology Vol 4 No 1 (2016)
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University in cooperation with Asia-Oceania Bioscience and Biotechnology Consortium (AOBBC)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (257.082 KB)

Phosphatase in the soil was found as extracellular enzymes produced by soil micro-organisms both in acidic or alkaline conditions. Phosphatase is an enzyme complex that plays an important role in deciding soil-bound phosphate bond organic compounds to form orthophosphate which is available to the plant. Mineralization of organic forms into a inorganic-P determined by the ability of these bacteria to produce phosphatase. Therefore, observations of phosphatase activities are important to know how intensely P mineralization process takes place in the soil. Phosphate solubilizing rhizobacteria (PSR) has been widely known to affect mobilization of insoluble inorganic phosphates become available to plants. Although phosphate solubilizing rhizobacteria is widely available in the soil, their activity is usually not strong enough to compete with other bacteria commonly located in the root zone (rhizosphere). That is why, screening of phosphatase enzyme activity and various phosphate solubilizing ability to obtain better strains of bacteria are still needed. This study was conducted to determine the ability of phosphate solubilizing rhizobacteria, its potential as a biofertilizer inoculant and characterize its capacity to dissolve P-insoluble inorganic and produce phosphatase enzymes in the growing medium. Potential phosphate solubilizing rhizobacteria was tested for their ability in vitro using Pikovskaya media containing insoluble forms of inorganic phosphate as a source P. The results of this study indicate that there are some isolates phosphate solubilizing rhizobacteria that are capable of dissolving the insoluble inorganic-P with different abilities. The highest potential successively displayed by isolates TbPP-4.1; BdPP-2.1; and SBPP-1.3 that significantly has the same ability to soluble inorganic phosphate in the soil. The isolates BdPP-2.1 and TbPP-4.1 are significantly higher than the other isolates for producing phosphatase. Both isolates TbPP-4.1 and BdPP-2.1 have the same pontenty as a biofertilizer based on its capacity to mineralize forms of soil organic phosphorus by phosphatase activity.

POTENTIAL EFFECT OF MACRO ALGA Caulerpa sp. AND Gracilaria sp. EXTRACT LOWERING MALONDIALDEHYDE LEVEL OF WISTAR RATS FED HIGH CHOLESTEROL DIET Ketut Srie Marhaeni Julyasih; I Gede Putu Wirawan
International Journal of Biosciences and Biotechnology Vol 5 No 1 (2017)
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University in cooperation with Asia-Oceania Bioscience and Biotechnology Consortium (AOBBC)

Seaweed has potential nutrient content such as carotenoids, vitamins, fatty acids, carbohydrates, minerals, and other essential substances. Carotenoids have important biological functions as an antioxidant, and immunostimulatory which can prevent the disease, anti-inflammatory, anti-stress, anti-aging, and protect the skin from the harmful effects of ultraviolet radiation. Seaweed generally consumed as a vegetable by people in Bali, known as the local name Bulung Boni (Caulerpa spp.) and Bulung Sangu (Gracilaria spp.).. So far there has been no report or results of research on the effects of extract ethanol of Bulung Boni (Caulerpa sp.) and Bulung Sangu (Gracilaria sp.) as an antioxidant that can prevent lipid peroxidation which can be seen in decreased level of MDA in liver tissue or blood plasma. Therefore it is necessary to determine of plasmaMDA level of Wistar rat after fed high cholesterol diet treated with extract ethanol of Caulerpa sp. and Gracillaria sp. This experimental study used completely randomized design. Research using total of 24 Wistar rats divided into six sample groups of equal size, all fed with a diet high in cholesterol especially in negative control. The study consisted of negative control group (standard diet), positive control group (high cholesterol diet), high-cholesterol diet with Caulerpa sp. extract dose of 20 mg and 60 mg/100 g, high cholesterol diet with Gracilaria sp. extract dose of 20 mg and 60 mg/100 g body weight rat per day.The study resulted that rats fed high cholesterol diet with treated extract ethanol Caulerpa sp. and Gracilaria sp. with a dose of 20 mg and 60 mg per 100 g body weight rat / day had plasma MDA level significantly lower (p <0.05) compared with rats fed high cholesterol diet without treated with extract of Caulerpa sp. and Gracilaria sp.

DISTRIBUTION OF CVPDr GENE AMONG SOME CITRUS PLANTS IN BALI I Gede Putu Wirawan
International Journal of Biosciences and Biotechnology Vol 3 No 2 (2016)
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University in cooperation with Asia-Oceania Bioscience and Biotechnology Consortium (AOBBC)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (207.783 KB)

Citrus Vein Phloem Degeneration (CVPD) is the main disease of citrus plant caused by a Gram negativebacteria, Candidatus Liberobacter asiaticus. The disease is also called citrus greening disease or recentlycalled citrus huanglongbing. The CVPDr gene was firstly found in Triphacia trifoliata a citrus relativeplant which considered to be resistant to CVPD disease. The distribution of the gene among the citrusrelative plants in Bali were studied. The result of this study so far, CVPDr gene were found in theseedless lime (Citrus aurantiifolia) and Triphacia trifoliata which are known as a resistant or tolerant toCVPD disease, however, Citrus maxima, Citrus nobilis, Citrus reticulate, and Citrus amblycarpa are allsensitive plants but their harboring the CVPDr gene. This result indicated that CVPDr gene was not givea resistant to the disease in these plants. This results suggested that other/s gene is needed to give aresistant or tolerant in these plants.

IDENTIFICATION OF ARBUSCULAR MYCORRHIZAL FUNGI IN RHIZOSPHERE SOIL OF SEVERAL GRASS SPECIES AND CACAO (Theobroma cacao L.) BASED ON ITS SPORE MORPHOLOGICAL CHARACTERISTICS Ni Kadek Sintya Dewi; I Gede Ketut Susrama; Made Sritamin; Made Adnyana; I Gede Putu Wirawan
International Journal of Biosciences and Biotechnology Vol 2 No 1 (2014)
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University in cooperation with Asia-Oceania Bioscience and Biotechnology Consortium (AOBBC)

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Arbuscular mycorrhizal fungi live in a symbiotic mutualism with plant roots and helps plant absorbnutrients and also able to live in various places. The study aimed to identify arbuscular mycorrhizal fungiin rizhosphere of several grass and cacao through microscopic method based on morphologicalcharacteristics and was conducted from December 2013 to March 2014. The methods used in this studywere spore isolation using wet sieving and root staining techniques. The results showed that arbuscularmycorrhizal fungi spores found in Imperata cylindrica L. were spores of Glomus (Glomales: Glomeaceae)with its vesicular and hyphae structure. The presence of spores and structures in Paspalum notatum wereAcaulospora (Glomales: Acaulosporaceae) and Gigaspora with its arbuscules, vesicules and hyphae structure.Spores and structures found in the Pennisetum purpureum were belong to genus of Glomus with hyphaeand arbuscular structure. Spores and structures of mycorrhizal fungi in Cyperus rotundus are spores of thegenus of Gigaspora (Glomales: Gigasporineae) and spores of Glomus with internal hyphae structure. Whilespores and mycorrhizal structures in Cacao were found two types of spore belong to genus of Glomus withhyphae and vesicular structures.

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